The aim of the study was to investigate the influence of physical conditions such as aeration rate (1, 3, 5 L/min) as well as chemical conditions including sodium nitrite (NaNO2), urea (CH4N2O) and ammonium chloride (NH4Cl) on the biomass productivity and fucoxanthin concentration of A. capitellata. The optimum cultures were cultivated in f/2 medium using sodium nitrate (NaNO3) in 2 L bubbling bottle photobioreactors under the light intensity of 100 µE/ m2s with aeration rate of 2 L/min. All the bottles were then incubated at 22.0±2°C, under the light intensities of 300 µE/m2s with three different airflow rates of 1, 3, 5 L/min for 16 days. And then, culture medium was prepared with three different nitrogen sources to achieve higher biomass productivity. During the production of A. capitellata, the maximum specific growth rate of 0.166 day-1, which conformed to the doubling time of 4.166 day, was achieved at the light intensity of 300 µE/m2s with an aeration rate of 1 L/min when sodium nitrate was used. Chlorophyll-a and fucoxanthin contents were also at the highest level in the same light intensity. Dry biomass amount reached the maximum level of 0.66±0.17 g/L in case of NaNO2. In this study, it was defined that the airflow rate of 1 L/min, the light intensity of 300 µE/m2s and sodium nitrate (NaNO3) were the optimum values not only for the growth of A. capitellata cells but also for the production of biomass and a higher fucoxanthin concentration.