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Abstract: The interaction of daunorubicin (DNR) with herring sperm DNA in cyclomaltooctaose (γ-cyclodextrin, abbrev. γ-CD) supramolecular system was studied by UV–vis absorption spectroscopy, fluorescence spectroscopy and viscosity method. On the condition of physiological pH, the results indicate that DNR prefers to form the 1:1 inclusion complex with γ-CD. These results are further supported by infrared spectrometry (IR), and X-ray diffraction (XRD). An inclusion constant (K) for the formation of γ-CD–DNR inclusion complex has been evaluated to be 9412.39Lmol−1 from the absorbance changes. It is observed that the interaction mode between γ-CD–DNR and DNA are a mixed binding, which contains partial intercalation and groove binding. The binding ratio of γ-CD–DNR with DNA is n γ-CD–DNR:n DNA =10:1, binding constant is K Θ 298.15K =2.44×105 Lmol−1, enthalpy and entropy work as driven force in this action. [Copyright &y& Elsevier]

Ultraviolet-visible (UV-vis) spectra, fluorescence spectra, electrochemistry, and the thermodynamic method were used to discuss the interaction mode between the inclusion complex of hematoxylin with β-cyclodextrin and herring sperm DNA. On the condition of physiological pH, the result showed that hematoxylin and β-cyclodextrin formed an inclusion complex with binding ratio nhematoxylin:nβ-cyclodextrin = 1:1. The interaction mode between β-cyclodextrin-hematoxylin and DNA was a mixed binding, which contained intercalation and electrostatic mode. The binding ratio between β-cyclodextrin-hematoxylin and DNA was nβ-cyclodextrin -hematoxylin:nDNA = 2:1, binding constant was K⊖298.15K = 5.29 × 104 L·mol-1, and entropy worked as driven force in this action. [ABSTRACT FROM AUTHOR]