10,548 results
Search Results
52. Research Paper The effect of inhalant anesthetic and body temperature on peri-anesthetic serum concentrations of transdermally administered fentanyl in dogs.
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Pettifer, Glenn R. and Hosgood, Giselle
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ANESTHETICS , *PHARMACODYNAMICS , *SERUM , *HYPOTHERMIA , *FENTANYL , *ISOFLURANE , *BLOOD pressure measurement , *LABORATORY dogs - Abstract
To determine whether moderate hypothermia during anesthesia significantly affects the serum concentration of transdermally delivered fentanyl and whether halothane or isoflurane affect these concentrations. Randomized cross-over experimental trial. Six mature, healthy Beagles (three males, three females) weighing 10.6 ± 0.43 kg. A 50-µg hour−1 fentanyl patch was applied 36 hours prior to anesthesia. Anesthesia was induced at time 0 ( t = 0). Each dog received four treatments: isoflurane + normothermia (ISO-NORM), isoflurane + hypothermia (ISO-HYPO), halothane + normothermia (HAL-NORM), and halothane + hypothermia (HAL-HYPO). Dogs were intubated and maintained at 1.5 times MAC. Animals in the hypothermia treatments were cooled to 35 °C during anesthesia. Serum fentanyl analysis was performed at −36, −24, −12, 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 18, and 26 hours. Direct arterial blood pressures and arterial blood gases were monitored. The mean body temperatures (±SEM) during the anesthetic period for the four treatments were: ISO-NORM = 37.7 ± 0.07 °C, ISO-HYPO = 35.8 ± 0.1 °C, HAL-NORM = 37.7 ± 0.06 °C, and HAL-HYPO = 35.8 ± 0.13 °C. The mean (±SEM) serum fentanyl concentrations (SFC) for both hypothermia treatments were significantly lower than baseline concentrations at t = 1 hour and persisted for the duration of anesthesia for the ISO-HYPO treatment but only from t = 1 to 2 hours for the HAL-HYPO treatment. Serum fentanyl concentrations returned to baseline within one hour of the end of anesthesia, regardless of body temperature. There were no significant differences between treatments for systolic or diastolic blood pressure but mean blood pressures were higher during normothermia versus hypothermia during the last hour of anesthesia. Hypothermia during inhalation anesthesia produced a significant reduction in SFC using transdermal administration and was more protracted with isoflurane than halothane anesthesia. While significant reductions in SFC occurred, the SFC were still within the range believed to confer analgesia. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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53. THE FIGHT IN THE DOG: Onfire Design, a branding agency based in Auckland, has built a reputation for its innovative designs and commitment to getting cut-through in the busy FMCG sector.
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REPUTATION ,DOGS ,BRAND name products ,BICYCLE stores ,FAST moving consumer goods ,PAPER towels - Published
- 2024
54. Radiography is less sensitive relative to CT for detecting thoracic radiographic changes in dogs affected by blunt trauma secondary to a motor vehicle accident.
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Dancer SC, Le Roux C, Fosgate GT, and Kirberger RM
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- Animals, Female, Injury Severity Score, Male, Prospective Studies, Radiography, Thoracic veterinary, Sensitivity and Specificity, Thoracic Injuries diagnostic imaging, Tomography, X-Ray Computed veterinary, Wounds, Nonpenetrating diagnostic imaging, Accidents, Traffic, Dogs injuries, Thoracic Injuries veterinary, Wounds, Nonpenetrating veterinary
- Abstract
Thoracic injuries caused by blunt trauma are commonly encountered emergencies in veterinary medicine. However, published studies are lacking that compare radiology to CT in blunt trauma caused by motor vehicle accidents in canine patients. The aim of this prospective diagnostic accuracy, methods comparison study were to estimate the sensitivity (Se) and specificity (Sp) of thoracic radiology relative to CT for detecting lung contusions, pneumothorax, pleural effusion, and rib fractures. The study further aimed to develop a severity scoring system for radiology and CT and to compare the findings between the two modalities. The hypothesis was that radiology would be less sensitive than CT at detecting these injuries and that radiology would underestimate the severity of lung contusions. Fifty-nine patients met the inclusion criteria. Radiology underestimated the presence of lung contusions (Se = 69%, 95% confidence interval) and overestimated the severity of the contusions relative to CT. There was high interobserver variability in evaluating lung contusion severity (coefficient of variation = 91%). Both the three-view thoracic and horizontal beam radiography had poor sensitivities for the detecting pneumothorax (Se = 19% and 63%, respectively) and pleural effusions (Se = 43% and 71%, respectively). Similarly, the sensitivity (56%) of radiographs for the detection of rib fractures was poor relative to CT. Findings from the current study indicated that thoracic radiography had low sensitivity for detecting lesions related to blunt thoracic trauma caused by motor vehicle accidents and supported the use of CT as an additional diagnostic imaging modality in these patients., (© 2019 American College of Veterinary Radiology.)
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- 2019
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55. REGARDING GRAEVSKII AND KEILINA'S PAPER "REDUCED SENSITIVITY TO LETHAL DOSES OF X-RAYS IN ANIMALS PREVIOUSLY GIVEN SUBETHAL DOSES"
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Yarmonenko, S
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- 1958
56. The National Academy of Sciences. Abstracts of Papers Presented at the 1949 Autumn Meeting, Rochester, New York
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- 1949
57. Are your dog's papers in order?
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Dogs ,General interest ,News, opinion and commentary - Abstract
Byline: LES PERREAUX CANINE CONTROL MONTREAL -- City tags dog? Nothing new there, but what if the city licenses the dog's keeper instead? Montreal's wealthy town-within-the-city called Westmount has found [...]
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- 2011
58. First Detection of Lactococcus petauri in Domestic Dogs in Italy.
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Sciuto, Simona, Esposito, Giuseppe, Pastorino, Paolo, Shahin, Khalid, Varello, Katia, Trabunella, Eliana, Milanese, Giulia, Scala, Sonia, Prearo, Marino, Acutis, Pier Luigi, Salerno, Angelo, Zoppi, Simona, and Colussi, Silvia
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DRUG resistance in bacteria ,VIRAL tropism ,URINARY tract infections ,DOGS ,FOOD contamination - Abstract
Simple Summary: Lactococcus petauri has been described for the first time in 2017 in a sugar glider. A few years later, it has emerged as important pathogen for fish and more recently it has been reported also in terrestrial mammals and humans. A lot of these cases were misdiagnosed as Lactococcus garvieae infection due to the limitation of the available common standard diagnostic techniques used which were unable to discriminate between these two species and relatively high similarity of the two pathogens at genetic and phenotypic levels. Till today, there are only two reported cases of lactococcosis in dogs with no previous cases were reported from Italy. This report is the first description of L. petauri infection in two domestics dog cases from Italy. The results of the current report provide an update on the host tropism, and additional knowledge on virulence factors and antimicrobial resistance of L. petauri. Lactococcus garvieae has been considered for a long time the only causal agent of lactococcosis. In recent years, different papers reported the involvement of other two bacterial species: Lactococcus petauri and Lactococcus formosensis. A different host tropism has been described for these species where L. garvieae and L. petauri are predominant species in fish and humans' infections, while L. formosensis in bovine. L. garvieae has been reported as rare infectious agent in dog. This paper represents the first isolation of L. petauri in two domesticated dog cases from urine and skin samples, respectively. The recovered L. petauri has been identified using PCR and sequencing based on Internal Transcribe Spacer (ITS) and phylogenetic analysis showed that it belongs to the L. petauri cluster with a 100% of identity with sequences previously reported from fish isolates while there were differences with L. petauri isolated from urinary tract infection from humans. L. petauri in human infection has been considered not necessarily deriving from the ingestion of contaminated food but rather as an opportunistic pathogen colonization intestinal tract. Differences among virulotypes have been reported for humans and dogs, and a comparison was also made between the virulotyping of L. petauri and L. garvieae in dogs. The antimicrobial pattern showed susceptibility for the election treatment molecules. These data contribute to our understanding of the host trophism of this species which was misclassified for long time and provide new data on its virulence factors and antimicrobial resistance. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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59. A comparison of undergraduate students' physical activity levels in a standard fitness walking class vs. a service-learning dog walking class.
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Sartore-Baldwin, Melanie L. and Das, Bhibha M.
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INTERDISCIPLINARY education ,SERVICE animals ,UNDERGRADUATES ,UNIVERSITIES & colleges ,PHYSICAL education ,DESCRIPTIVE statistics ,MULTIVARIATE analysis ,GAIT in humans ,DOGS ,WALKING ,PEDOMETERS ,PHYSICAL fitness ,SERVICE learning ,ANALYSIS of variance ,STATISTICS ,DATA analysis software ,HEALTH education ,COLLEGE students ,COMPARATIVE studies ,PHYSICAL activity - Abstract
Objective: To investigate the physical activity levels of students enrolled in a standard fitness walking class versus a service-learning dog walking class. Participants: College students (N = 65) from a university in the Southeastern United States (71% female; 85% White; 72% seniors). Methods: Students wore NL-1000 pedometers twice a week for 50 minutes for a full academic year. Service-learning students also completed reflection papers. Pedometer data was analyzed using SPSS and papers were analyzed through inductive coding. Results: Significant differences were found in each quantitative measure collected between classes. Students in the service-learning fitness walking class took more steps, walked further, and obtained more minutes of moderate to vigorous physical activity than the students in the standard fitness walking class. Results from student reflections provided insight into why these differences occurred as well as additional student experiences. Conclusions: Incorporating a service component into a physical activity course can be beneficial. [ABSTRACT FROM AUTHOR]
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- 2024
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60. The Paper Roo: Sherman Edition
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Dogs ,News, opinion and commentary ,Sports and fitness - Abstract
I know I'm not the only one who gets really really excited when there's a dog on campus. Like, really excited. I miss my dog at home probably more than [...]
- Published
- 2014
61. The truth about men and dogs: why can't a man be more like a dog? Woof!--he is! In fact, the mutt in your life may be uncannily similar to the guy asleep on the sofa, fetching the paper, or jogging by your side in the park. Vicki Croke bones up on a pet theory
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Croke, Vicki
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Motion picture industry ,Dogs ,Motion pictures ,Dogs in motion pictures - Abstract
CYNTHIA MOSES, A WILDLIFE DOCUMENTARY FILM-maker in Washington, D.C., has dated all kinds of men--quick-tempered artists, uptight lawyers, and self-absorbed businessmen. But the 55-year-old is happy at last, involved in […]
- Published
- 2005
62. Metro Mutts Wins Two Top Honors inWashington DC City Paper's 2013 ReadersPoll
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Pet industry ,Dogs ,General interest - Abstract
India, Aug. 6 -- Metro Mutts was awarded high honors in two pet-enthusiast categories, according to results announced last week by Washington City Paper's Best of DC 2013 Readers Poll.Metro [...]
- Published
- 2013
63. Sensitivity of a Hymenoptera serological immunoglobulin (Ig)E assay for the diagnosis of venom hypersensitivity in dogs.
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Chan, Hilary H., Burrows, Amanda K., Hosgood, Giselle, and Ghubash, Rudayna
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VENOM hypersensitivity ,HYMENOPTERA ,DOGS ,HONEYBEES ,RECEIVER operating characteristic curves ,POLLINATION ,INSECT bites & stings ,DOG walking ,BEES - Abstract
Copyright of Veterinary Dermatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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64. PAPER CHROMATOGRAPHY OF STEROIDS
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Kirk, M
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- 1952
65. WHITE PAPER REVIEWS THE STATE OF GENETIC TESTING IN DOGS.
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GOVERNMENT report writing , *GENETIC testing , *DOGS , *DOG breeds - Abstract
The article reports that the American Kennel Club Canine Health Foundation announced that it has published a review of the current state of genetic testing in dogs to help dog breeders, dog owners, and veterinarians make sound decisions with regard to interpreting and understanding the implications of genetic test results.
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- 2020
66. Vietnam memorial open to all, but dogs given walking papers
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Dogs ,Business ,Business, regional ,General interest - Published
- 2006
67. Rabies control in Ghana: Stakeholders interventions, challenges and opportunities.
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Emikpe, Benjamin Obukowho, Asare, Derrick Adu, Tasiame, William, Segbaya, Sylvester, Takyi, Prince Nana, and Allegye‐Cudjoe, Emmanuel
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PUBLIC health ,HEALTH services accessibility ,MEDICAL care ,DOGS ,VACCINATION coverage - Abstract
Background: Rabies remains a significant public health issue in Ghana, predominantly affecting rural communities with limited access to health care and veterinary services. The disease is primarily transmitted through bites from infected domestic dogs and leads to many deaths worldwide each year. Despite various interventions, Ghana continues to struggle with rabies control and prevention. This narrative paper focuses on rabies control in Ghana, examining stakeholders' interventions, challenges faced, and opportunities available. Methods: The paper reviews existing rabies control measures in Ghana, including dog vaccination efforts and One Health strategies. It also discusses the limitations of these interventions, such as minimal vaccination coverage, weak surveillance, and inadequate intersectoral coordination. Results: Ghana has implemented dog vaccination campaigns, but coverage remains minimal. Additionally, weak disease surveillance, cultural perceptions, and inadequate coordination across sectors have hampered the country's ability to control rabies effectively. Conclusion: Adopting One Health principles, which integrate human, animal, and environmental health efforts, is emphasized as a critical strategy to eliminate rabies in Ghana by 2030. By addressing these challenges and leveraging available opportunities, Ghana can strengthen its rabies control programs and work toward a rabies‐free future. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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68. Application of Ultrasound in Detecting and Removing Migrating Grass Awns in Dogs and Cats: A Systematic Review.
- Author
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Caivano, Domenico, Corda, Francesca, Corda, Andrea, Moretti, Giulia, and Bufalari, Antonello
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WHEATGRASSES ,CAT diseases ,FOREIGN bodies ,ULTRASONIC imaging ,DOG diseases ,DOGS ,FELIDAE - Abstract
Simple Summary: The aim of this systematic review was to summarize the current knowledge on the application of ultrasonography in the identification and removal of grass awns from various anatomic locations in dogs and cats. We selected and analyzed 46 papers on the application of ultrasonography in dogs and cats affected by migrating grass awns. The ultrasonographic appearance of grass awns is characteristic, although their size and location can influence visualization and removal attempt. In some cases, migrating grass awns are not directly visualized by ultrasonography, but lesions caused by their migration can be easily seen. Ultrasonography can be considered a useful diagnostic tool to localize and remove migrating grass awns; however, when the migration occurs in less accessible locations or discrete foreign bodies are present, this diagnostic tool should be considered as a part of a multidisciplinary approach with advanced diagnostic imaging modalities. Migrating grass awns are an important cause of disease in dogs and cats. Plant awns can migrate into several body tissues and cavities because of their fusiform shape and backward-pointing barbs. Their migration causes inflammatory tissue reaction and clinical signs depend upon their localization. Ultrasound has been described as a useful, noninvasive, and readily available tool to identify and guide vegetal foreign bodies removal in animals. The aim of this systematic review was to summarize current knowledge on the application of ultrasonography in the identification and removal of grass awns from various anatomic locations in dogs and cats. We selected and analyzed 46 papers on the application of ultrasonography in dogs and cats affected by migrating grass awns. The ultrasonographic appearance of grass awns is characteristic, although their size and location can influence the visualization and the attempt of removal. In some cases, migrating grass awns are not directly visualized by ultrasonography, but the lesions caused by their migration can be easily seen. Ultrasonography can be considered a useful diagnostic tool to localize and remove migrating grass awns; however, when the migration occurs in less accessible locations or discrete foreign bodies are present, this diagnostic tool should be considered as a part of a multidisciplinary approach with advanced diagnostic imaging modalities. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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69. Man In India Marries Dog As Atonement: He Tells Paper He Had Been Suffering Since He Stoned Two Canines To Death.
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Selvakumar, P.
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DOGS ,HUMAN-animal relationships ,MARRIAGE ,HINDU marriage customs & rites - Abstract
The article reports on the marriage of 33-year-old P. Selvakumar and the sari-draped former stray named Selvi in Tamil Nadu, India. It notes that Selvakumar married the female dog in a traditional Hindu ceremony as an attempt to ward off certain curses for stoning two other dogs to death. It states that the legs and hands of Selvakumar got paralyzed and he lost hearing his one ear since he stoned two dogs to death and hung their bodies from a tree.
- Published
- 2012
70. A low cost, safe, disposable, rapid and self-sustainable paper-based platform for diagnostic testing: lab-on-paper
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David S. Santos, Bruno Veigas, J. Jacob, Pedro V. Baptista, Rodrigo Martins, Mafalda Costa, Jacinto Gomes, Elvira Fortunato, and João Inácio
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Paper ,Materials science ,Microfluidics ,Nanoprobe ,Enzyme-Linked Immunosorbent Assay ,Bioengineering ,Nanotechnology ,02 engineering and technology ,Calorimetry ,01 natural sciences ,Dogs ,Animals ,Humans ,General Materials Science ,Pathology, Molecular ,Electrical and Electronic Engineering ,Leishmaniasis ,biology ,Molecular Diagnostic Testing ,Mechanical Engineering ,010401 analytical chemistry ,Glucose detection ,Collodion ,Nucleic Acid Hybridization ,Reproducibility of Results ,Diagnostic test ,Mycobacterium tuberculosis ,General Chemistry ,Paper based ,021001 nanoscience & nanotechnology ,biology.organism_classification ,0104 chemical sciences ,Glucose ,Mycobacterium tuberculosis complex ,Mechanics of Materials ,Homogeneous ,0210 nano-technology - Abstract
There is a strong interest in the use of biopolymers in the electronic and biomedical industries, mainly towards low-cost applications. The possibility of developing entirely new kinds of products based on cellulose is of current interest, in order to enhance and to add new functionalities to conventional paper-based products. We present our results towards the development of paper-based microfluidics for molecular diagnostic testing. Paper properties were evaluated and compared to nitrocellulose, the most commonly used material in lateral flow and other rapid tests. Focusing on the use of paper as a substrate for microfluidic applications, through an eco-friendly wax-printing technology, we present three main and distinct colorimetric approaches: (i) enzymatic reactions (glucose detection); (ii) immunoassays (antibodies anti-Leishmania detection); (iii) nucleic acid sequence identification (Mycobacterium tuberculosis complex detection). Colorimetric glucose quantification was achieved through enzymatic reactions performed within specific zones of the paper-based device. The colouration achieved increased with growing glucose concentration and was highly homogeneous, covering all the surface of the paper reaction zones in a 3D sensor format. These devices showed a major advantage when compared to the 2D lateral flow glucose sensors, where some carryover of the coloured products usually occurs. The detection of anti-Leishmania antibodies in canine sera was conceptually achieved using a paper-based 96-well enzyme-linked immunosorbent assay format. However, optimization is still needed for this test, regarding the efficiency of the immobilization of antigens on the cellulose fibres. The detection of Mycobacterium tuberculosis nucleic acids integrated with a non-cross-linking gold nanoprobe detection scheme was also achieved in a wax-printed 384-well paper-based microplate, by the hybridization with a species-specific probe. The obtained results with the above-mentioned proof-of-concept sensors are thus promising towards the future development of simple and cost-effective paper-based diagnostic devices.
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71. Estimation of Sexual Dimorphism in a Population of Dogs of the Romanian Mioritic Shepherd Dog Breed.
- Author
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Dronca, Dorel, Pet, Ioan, Dumitrescu, Gabi, Ștef, Lavinia, Petculescu, Liliana Ciochină, Silvia, Pătruică, Ivancia, Mihaela, Simiz, Eliza, Maftei, Marius, Nicula, Marioara, Marcu, Adela, Cazacu, Mihaela, Erina, Silvia, and Ahmadi, Mirela
- Subjects
SEXUAL dimorphism ,SPAWNING ,STATISTICAL significance ,ROMANIANS ,LENGTH measurement ,DOG breeds ,DOGS - Abstract
Romanian Mioritic Shepherd Dog, was selected from a natural population breed of Romanian Carpathian Mountains. The aim of this study was to analyze the existence and size of sexual dimorphism in a population of 26 males and 23 females of the Mioritic Shepherd Dog breed, for 6 body measurements: ear length, ear width, distance between the ears, distance between the eyes, length hair at withers and metacarpal perimeter. Following the study on the significance of statistical differences between body measurements recorded in 26 males and 23 females, it was concluded that sexual dimorphism is not evident in the population of the Romanian Mioritic Shepherd Dog studied in this paper, except the distance between the ears character. Among the other characters, the differences between the individuals of the two sexes are insignificant (p>0.05). We recommend to the dog breeders to take into account the genetic improvement programs, and also the results presented in this paper. [ABSTRACT FROM AUTHOR]
- Published
- 2022
72. Roan, ticked and clear coat patterns in the canine are associated with three haplotypes near usherin on CFA38
- Author
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Kerstin Lindblad-Toh, Claire M. Wade, Cali E. Willet, Hamutal Mazrier, Lillian Brancalion, Frode Lingaas, and Bianca Haase
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0301 basic medicine ,coat‐color ,Male ,Coat ,usherin ,Genotype ,Locus (genetics) ,Biology ,03 medical and health sciences ,USH2A ,Dogs ,Genetics ,Missense mutation ,Animals ,pigmentation ,coat-color ,Allele ,Genetik ,Hair Color ,Gene ,Alleles ,Genetic Association Studies ,Full Paper ,Haplotype ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Full Papers ,040201 dairy & animal science ,White (mutation) ,030104 developmental biology ,Phenotype ,Haplotypes ,Epistasis ,Animal Science and Zoology ,Female - Abstract
White coat patterning is a feature of many dog breeds and is known to be coded primarily by the gene micropthalmia-associated transcription factor (MITF). This patterning in the coat can be modified by other factors to produce the attractive phenotypes termed ‘ticked’ and ‘roan’ that describe the presence of flecks of color that vary in distribution and intensity within otherwise ‘clear’ white markings. The appearance of the pigment in the white patterning caused by ticking and roaning intensifies in the weeks after birth. We applied genome-wide association to compare English Cocker Spaniels of roan phenotype (N = 34) with parti-color (non-roan) English Cocker Spaniels (N = 9) and identified an associated locus on CFA 38, CFA38:11 057 040 (Praw = 8.9 × 10−10, Pgenome = 2.7 × 10−5). A local case–control association in English Springer Spaniels comparing 11 ticked and six clear dogs identified indicative association with a different haplotype, CFA38:11 122 467G>T (Praw = 1.7 × 10−5) and CFA38:11 124 294A>C (Praw = 1.7 × 10−5). We characterize three haplotypes in Spaniels according to their putative functional variant profiles at CFA38:11 111 286C>T (missense), CFA38:11 131 841–11 143 239DUP.insTTAA (using strongly linked marker CFA38:11 143 243C>T) and CFA38:11 156 425T>C (splice site). In Spaniels, the haplotypes work as an allelic series including alleles (t, recessive clear; T, dominant ticked/parti-color; and TR, incomplete dominant roan) to control the appearance of pigmented spots or flecks in otherwise white areas of the canine coat. In Spaniels the associated haplotypes are t (CCT), T (TCC) and TR (TTT) for SNP markers on CFA38 at 11 111 286C>T, 11 143 243C>T and 11 156 425T>C respectively. It is likely that other alleles exist in this series and together the haplotypes result in a complex range of patterning that is only visible when dogs have white patterning resulting from the epistatic gene Micropthalmia-associated transcription factor (the S-locus).
- Published
- 2021
73. Structures and therapeutic potential of anti-RBD human monoclonal antibodies against SARS-CoV-2
- Author
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Kuan-Ying A. Huang, Daming Zhou, Tiong Kit Tan, Charles Chen, Helen M. E. Duyvesteyn, Yuguang Zhao, Helen M. Ginn, Ling Qin, Pramila Rijal, Lisa Schimanski, Robert Donat, Adam Harding, Javier Gilbert-Jaramillo, William James, Julia A. Tree, Karen Buttigieg, Miles Carroll, Sue Charlton, Chia-En Lien, Meei-Yun Lin, Cheng-Pin Chen, Shu-Hsing Cheng, Xiaorui Chen, Tzou-Yien Lin, Elizabeth E. Fry, Jingshan Ren, Che Ma, Alain R. Townsend, and David I. Stuart
- Subjects
medicine.drug_class ,Medicine (miscellaneous) ,Receptor-binding domain epitope ,Monoclonal antibody ,medicine.disease_cause ,Crystallography, X-Ray ,Binding, Competitive ,Epitope ,Neutralization ,Madin Darby Canine Kidney Cells ,Epitopes ,Dogs ,Protein Domains ,Neutralization Tests ,Cricetinae ,medicine ,Animals ,Humans ,Viral shedding ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Mutation ,Binding Sites ,biology ,SARS-CoV-2 ,Cryoelectron Microscopy ,Antibodies, Monoclonal ,COVID-19 ,Antibody cocktail ,Human monoclonal antibody ,Virology ,Antibodies, Neutralizing ,In vitro ,COVID-19 Drug Treatment ,Antibody-antigen complex ,Spike Glycoprotein, Coronavirus ,biology.protein ,In vitro and in vivo function ,Female ,Angiotensin-Converting Enzyme 2 ,Antibody ,Viral load ,Research Paper - Abstract
Background: Administration of potent anti-receptor-binding domain (RBD) monoclonal antibodies has been shown to curtail viral shedding and reduce hospitalization in patients with SARS-CoV-2 infection. However, the structure-function analysis of potent human anti-RBD monoclonal antibodies and its links to the formulation of antibody cocktails remains largely elusive. Methods: Previously, we isolated a panel of neutralizing anti-RBD monoclonal antibodies from convalescent patients and showed their neutralization efficacy in vitro. Here, we elucidate the mechanism of action of antibodies and dissect antibodies at the epitope level, which leads to a formation of a potent antibody cocktail. Results: We found that representative antibodies which target non-overlapping epitopes are effective against wild type virus and recently emerging variants of concern, whilst being encoded by antibody genes with few somatic mutations. Neutralization is associated with the inhibition of binding of viral RBD to ACE2 and possibly of the subsequent fusion process. Structural analysis of representative antibodies, by cryo-electron microscopy and crystallography, reveals that they have some unique aspects that are of potential value while sharing some features in common with previously reported neutralizing monoclonal antibodies. For instance, one has a common VH 3-53 public variable region yet is unusually resilient to mutation at residue 501 of the RBD. We evaluate the in vivo efficacy of an antibody cocktail consisting of two potent non-competing anti-RBD antibodies in a Syrian hamster model. We demonstrate that the cocktail prevents weight loss, reduces lung viral load and attenuates pulmonary inflammation in hamsters in both prophylactic and therapeutic settings. Although neutralization of one of these antibodies is abrogated by the mutations of variant B.1.351, it is also possible to produce a bi-valent cocktail of antibodies both of which are resilient to variants B.1.1.7, B.1.351 and B.1.617.2. Conclusions: These findings support the up-to-date and rational design of an anti-RBD antibody cocktail as a therapeutic candidate against COVID-19.
- Published
- 2022
74. Seroprevalence of SARS-CoV-2 (COVID-19) exposure in pet cats and dogs in Minnesota, USA
- Author
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Daniel A. Heinrich, Yuying Liang, Shamim Ahmed, Hinh Ly, Da Di, Mythili Dileepan, and Qinfeng Huang
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viruses ,Infectious and parasitic diseases ,RC109-216 ,Antibodies, Viral ,medicine.disease_cause ,Neutralization ,Seroepidemiologic Studies ,skin and connective tissue diseases ,Coronavirus ,0303 health sciences ,CATS ,biology ,seroprevalence ,neutralization antibodies ,virus diseases ,Pets ,sars-cov-2 ,Infectious Diseases ,covid-19 ,Vesicular stomatitis virus ,Spike Glycoprotein, Coronavirus ,dog ,elisa ,Antibody ,Research Article ,Research Paper ,Microbiology (medical) ,Minnesota ,Immunology ,cat ,Enzyme-Linked Immunosorbent Assay ,Sensitivity and Specificity ,Microbiology ,COVID-19 Serological Testing ,feline coronaviruses ,03 medical and health sciences ,Dogs ,Antigen ,medicine ,Animals ,Coronavirus Nucleocapsid Proteins ,Seroprevalence ,Coronavirus, Feline ,030304 developmental biology ,030306 microbiology ,fungi ,Phosphoproteins ,biology.organism_classification ,Antibodies, Neutralizing ,Virology ,zoonoses ,body regions ,Cats ,biology.protein ,Parasitology - Abstract
The COVID-19 pandemic caused by the coronavirus SARS-CoV-2 is continuing to spread globally. SARS-CoV-2 infections of feline and canine species have also been reported. However, it is not entirely clear to what extent natural SARS-CoV-2 infection of pet dogs and cats is in households. We have developed enzyme-linked immunosorbent assays (ELISAs) using recombinant SARS-CoV-2 nucleocapsid (N) protein and the receptor-binding-domain (RBD) of the spike protein, and the SARS-CoV-2 spike-pseudotyped vesicular stomatitis virus (VSV)-based neutralization assay to screen serum samples of 239 pet cats and 510 pet dogs in Minnesota in the early phase of the COVID-19 pandemic from mid-April to early June 2020 for evidence of SARS-CoV-2 exposures. A cutoff value was used to identify the seropositive samples in each experiment. The average seroprevalence of N- and RBD-specific antibodies in pet cats were 8% and 3%, respectively. Among nineteen (19) N-seropositive cat sera, fifteen (15) exhibited neutralizing activity and seven (7) were also RBD-seropositive. The N-based ELISA is also specific and does not cross react with antigens of common feline coronaviruses. In contrast, SARS-CoV-2 antibodies were detected at a very low percentage in pet dogs (~ 1%) and were limited to IgG antibodies against SARS-CoV-2 N protein with no neutralizing activities. Our results demonstrate that SARS-CoV-2 seropositive rates are higher in pet cats than in pet dogs in MN early in the pandemic and that SARS-CoV-2 N-specific IgG antibodies can detect SARS-CoV-2 infections in companion animals with higher levels of specificity and sensitivity than RBD-specific IgG antibodies in ELISA-based assays.
- Published
- 2021
75. Case Report: A case study of positive doping control by animal-to-human drug transfer after an athlete administered medicine in spray format, containing clostebol acetate, to a pet dog.
- Author
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Pokrywka, Andrzej, Sitkowski, Dariusz, Surała, Olga, Gheddar, Laurie, and Kintz, Pascal
- Subjects
HAIR analysis ,DOPING agents (Chemistry) ,DOGS ,SUBSTANCE abuse ,FUR - Abstract
The presence of a doping substance in an athlete's biological sample may not be only related to intentional pharmacological support. The unintended use of a prohibited substance may be due various reasons. This paper describes the case of a Polish canoeist preparing for the 2024 Summer Olympics in Paris who presented a positive doping test result, as a consequence of administering medication to her injured dog. The athlete used a Trofodermin cutaneous spray (containing clostebol acetate) for pet treatment, which resulted in human transfer during close contact and subsequent detection by doping authorities. To bolster the athlete's defense, it was essential to substantiate the scenario of an unconscious violation of anti-doping rules with scientific evidence. Hence, the decision was made to analyze and compare samples of the athlete's hair and her dog's fur. This investigation confirmed that clostebol absorption occurred through the skin of the hands, transfer during sleeping with the dog on the same bedding and/or inhalation (during the application of the medication, which was dispensed to the animal's paws). This defense was accepted by the Court of Arbitration for the Sport Anti-Doping Division, which subsequently found that the athlete committed an anti-doping rule violation, but under circumstances that amounted to a "no fault" scenario. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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76. Talking Dogs: The Paradoxes Inherent in the Cultural Phenomenon of Soundboard Use by Dogs.
- Author
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Włodarczyk, Justyna, Harrison, Jack, Kruszona-Barełkowska, Sara L., and Wynne, Clive D. L.
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NONVERBAL communication ,DOG training ,TRAINING manuals ,HUMAN voice ,PARADOX ,DOGS ,SERVICE animals - Abstract
Simple Summary: Dogs that press buttons to communicate with people have attracted a lot of attention on social media recently. This paper explores how communicating with dogs has a long history—particularly as entertainment but also as science—and notes several paradoxes in the presentation of modern button-pressing dogs. These include how these animals are presented as spontaneously expressing their thoughts when they need months of training; how they appear to offer direct access to their thoughts, yet their button presses usually make little sense without someone interpreting them; and how a human skill—language—is needed for dogs to express their canine mental states. We conclude that although well intentioned and playful, this approach to communicating with dogs runs the risk of replacing the ways that dogs truly communicate with people, such as barking and whining, with an infantile form of human communication. In recent years, dogs that appear to communicate with people by pressing buttons on soundboards that replay pre-recorded English words have become very popular on social media online. We explore how these dogs belong to a historical tradition that dates back at least to the Middle Ages and peaked in the early twentieth century. Through analyses of short videos, books, and training manuals, we identify several paradoxes inherent in this phenomenon. These include how the dogs appear to provide unmediated access to their thoughts, and yet, their button presses are typically incoherent and require interpretation. They also require months of training to "spontaneously" express themselves. There is also an anthropomorphism and -centrism in claiming that a human skill—language—is required for a dog to express mental states that it already possesses. This approach to communicating with dogs quiets canine forms of expression such as barking, whining, bodily postures, and odors and replaces them with endearing but infantile human voices. We suggest that, while this endeavor may be well intentioned and often playful, it runs the risk of skewing people's perception of dogs towards fur-clad infants rather than adult members of a different species and of making people less attentive to canine nonverbal communication. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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77. Animal‐assisted services for adults with acquired neurogenic communication disorders: A scoping review.
- Author
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McSween, Marie‐Pier, Day, Tasman, Hill, Jessica, and Wallace, Sarah J.
- Subjects
- *
TREATMENT of communicative disorders , *MEDICAL information storage & retrieval systems , *COMMUNICATIVE competence , *PET therapy , *RESEARCH funding , *ACADEMIC medical centers , *CINAHL database , *TREATMENT effectiveness , *DOGS , *APHASIA , *EMOTIONS , *SYSTEMATIC reviews , *MEDLINE , *APRAXIA , *MOTIVATION (Psychology) , *MEDICAL databases , *SOCIAL skills , *ONLINE information services , *DATA analysis software , *AFFECT (Psychology) , *PATIENT satisfaction , *SPEECH therapy , *PSYCHOLOGY information storage & retrieval systems , *PATIENT participation , *EVALUATION , *ADULTS - Abstract
Background: There is increasing interest in the incorporation of animal‐assisted services (AAS) in therapy for adults with acquired neurogenic communication disorders. AAS have the potential to enhance speech and language therapy engagement and outcomes. However, a greater understanding of the nature and potential benefits of these interventions is needed. Aims: To describe the existing evidence for the incorporation of AAS in therapy with adults with acquired neurogenic communication disorders and to identify areas for future research. Methods & Procedures: A scoping review was conducted and reported in alignment with the Preferred Reporting Items for Systematic Reviews and Meta‐analyses checklist extension for scoping reviews (PRISMA‐ScR). Seven databases (PubMed, Embase, CINAHL, PsycINFO, Cochrane, Scopus, Web of Science) and grey literature (Google) were searched. Two reviewers independently screened titles, abstracts and full texts against eligibility criteria using Covidence software. The Template for Intervention Description and Replication (TIDieR) checklist guided extraction of intervention data. Main Contributions: A total of 17 studies with adults with aphasia, apraxia of speech and cognitive–communication disorders were included. While terminology varied, most interventions met the definition of animal‐assisted therapy or animal‐assisted activity and used therapy dogs. Across studies, a range of outcomes were targeted, and positive benefits were reported for participant mood, emotions, motivation and satisfaction. There were mixed, but mostly positive, benefits on social behaviour, communication and participation. Conclusions & Implications: AAS has been incorporated in therapy for adults with acquired neurogenic communication disorders, predominately with people with dementia. Across studies, communication impairments and AAS interventions were insufficiently or inconsistently described. Improved reporting would assist understanding of the potential benefits of AAS as an adjunct therapy. A quality appraisal of existing studies, and meta‐analysis of findings, is needed to draw conclusions about the effectiveness of AAS as a complementary therapy for people with acquired neurogenic communication disorders. WHAT THIS PAPER ADDS: What is already known on this subject: There is increasing interest and research in AAS as an adjunct to traditional speech and language therapy. Several clinical populations have been shown to benefit from the incorporation of AAS as a complementary therapy approach, including adults with acquired neurogenic communication disorders. To date there has not been a comprehensive review of literature in the area. What this paper adds to the existing knowledge: This review aimed to describe what is known about AAS as an adjunct intervention for adults with acquired neurogenic communication disorders. A total of 17 studies were identified, the majority conducted with people living with dementia. Overall, consistent positive benefits of AAS were reported on participant mood, positive emotions, motivation, and satisfaction. There were mixed, but mostly positive, benefits of AAS on social behaviour, including both verbal and non‐verbal communication outcomes, and participation. What are the practical and clinical implications of this work?: These results suggest a potential therapeutic benefit for the inclusion of AAS into traditional modalities used by speech–language therapists with people with acquired neurogenic communication disorders. However, clinicians should view these findings with caution. Studies were often insufficiently or inconsistently reported, and a quality appraisal and meta‐analysis of existing studies would be needed to draw clear conclusions on the effectiveness of AAS as a complementary approach for people with acquired neurogenic communication disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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78. Dis/Ability: A Discussion on Creating More Accessible Employment for Assistance Dog Instructors with Disabilities.
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Warda, Tiamat
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- *
WORKING dogs , *DOGS , *WELL-being , *EMPATHY , *EMPLOYMENT - Abstract
Disabled individuals can and do lead successful careers as assistance dog instructors. However, the international percentage of professional disabled instructors is significantly lower than their able-bodied colleagues. This paper takes an initial step in questioning the benefits and challenges of creating more accessible and inclusive career paths for disabled assistance dog instructors, hopefully acting as a springboard for future research, and initiating conversation within the assistance dog sector itself, as well. Some disabled individuals may have a disability, or combinations of more than one, which could challenge the welfare and wellbeing of the dogs they educate. Others may have a greater aptitude for educating assistance dogs and offering deeper empathy at times. This paper was inspired by the author's six years working as a guide dog instructor. It questions what the implications of a disabled trainer's career might be for the disabled individual, assistance dog, employer, and client. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
79. Testicular tumors in dogs.
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Efendić, M., Vnuk, D., Zagradišnik, L. Medven, Hohšteter, M., Kuleš, J., Musulin, A., and Maćešić, N.
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ANIMAL health ,DOGS ,BIOLOGICAL research ,TUMORS ,TESTOSTERONE - Abstract
Copyright of Veterinarska Stanica is the property of Croatian Veterinary Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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80. Two sides to every story: Reappraising the early history of liver transplantation at Addenbrooke's Hospital, Cambridge.
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Featherstone, Peter J
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LIVER transplantation ,LIVER failure ,DOG diseases ,LIVER diseases ,SURVIVAL rate - Abstract
Over the course of the past six decades, liver transplantation has evolved to become the treatment of choice for chronic end-stage liver disease and some cases of acute hepatic failure. Currently, more than 34,000 liver transplants are conducted worldwide per annum, and overall one year survival rates exceed 90%. However, the early years of human liver transplantation were beset by failure. Eyewitness reports from this period make for tragic, yet compelling reading. Volume 12 of The History of Anaesthesia Society Proceedings contains one such account, written by Dr Elizabeth Gibbs. This outlined the experience of single-handedly anaesthetising the recipient of the first liver transplant to be undertaken at Addenbrooke's Hospital, Cambridge, UK, in June 1967. Despite the best efforts of the team, the patient died from uncontrollable haemorrhage 19 hours after the start of the procedure. While this tragic outcome might have been expected in the early days of liver transplantation, Gibbs included an unsettling postscript in her paper. This stated that all of the patient's records had apparently been 'lost', and the date of the first Cambridge liver transplant was publicly reported as 2 May 1968, some 11 months after the events described. This article outlines the results of research aimed at critically appraising this postscript. In doing so a number of unexpected discoveries were made. These highlight some important lessons for medical historians and demonstrate that if you look hard enough, there really are two sides to every story. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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81. Classic Papers Revisited: An Early Study of Cardioprotection by Volatile Anesthetics: A Behind-the-scenes Look.
- Author
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Warltier, David C.
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- *
CORONARY heart disease prevention , *ANIMALS , *CARDIOTONIC agents , *CORONARY disease , *DOGS , *HALOTHANE , *ISOFLURANE , *INHALATION anesthetics - Abstract
Recovery of Contractile Function of Stunned Myocardium in Chronically Instrumented Dogs Is Enhanced by Halothane or Isoflurane. By Warltier DC, al-Wathiqui MH, Kampine JP, and Schmeling WT. ANESTHESIOLOGY 1988; 69:552-65. Reprinted with permission.Following brief periods (5-15 min) of total coronary artery occlusion and subsequent reperfusion, despite an absence of tissue necrosis, a decrement in contractile function of the postischemic myocardium may nevertheless be present for prolonged periods. This has been termed "stunned" myocardium to differentiate the condition from ischemia or infarction. Because the influence of volatile anesthetics on the recovery of postischemic, reperfused myocardium has yet to be studied, the purpose of this investigation was to compare the effects of halothane and isoflurane on systemic and regional hemodynamics following a brief coronary artery occlusion and reperfusion. Nine groups comprising 79 experiments were completed in 42 chronically instrumented dogs. In awake, unsedated dogs a 15-min coronary artery occlusion resulted in paradoxical systolic lengthening in the ischemic zone. Following reperfusion active systolic shortening slowly returned toward control levels but remained approximately 50% depressed from control at 5 h. In contrast, dogs anesthetized with halothane or isoflurane (2% inspired concentration) demonstrated complete recovery of function 3-5 h following reperfusion. Because the anesthetics directly depressed contractile function, additional experiments were conducted in which a 15-minute coronary artery occlusion was produced during volatile anesthesia; however, each animal was allowed to emerge from the anesthetized state at the onset of reperfusion. Similar results were obtained in these experiments, demonstrating total recovery of contractile function within 3-5 h following reperfusion. Thus, despite comparable degrees of contractile dysfunction during coronary artery occlusion in awake and anesthetized dogs, the present results demonstrate that halothane and isoflurane produce marked improvement in the recovery of segment function following a transient ischemic episode. Therefore, volatile anesthetics may attenuate postischemic left ventricular dysfunction occurring intraoperatively and enhance recovery of regional wall motion abnormalities during reperfusion. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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82. Clinical evaluation of urinary liver-type fatty acid-binding protein for the diagnosis of renal diseases in dogs
- Author
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Naohito Nishii, Keiichi Ohata, Tsuyoshi Oikawa, Takeshi Sugaya, Yui Kobatake, Yumiko Nagamori, and Satoshi Takashima
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medicine.medical_specialty ,Urinary system ,Urine ,urinary liver-type fatty acid-binding protein ,medicine.disease_cause ,Fatty Acid-Binding Proteins ,Gastroenterology ,chemistry.chemical_compound ,Dogs ,renal disease ,Internal medicine ,Internal Medicine ,Medicine ,Animals ,Dog Diseases ,Kidney ,Creatinine ,General Veterinary ,biology ,Full Paper ,Urine specific gravity ,business.industry ,medicine.anatomical_structure ,Alanine transaminase ,chemistry ,Liver ,dog ,biology.protein ,Biomarker (medicine) ,lipids (amino acids, peptides, and proteins) ,Kidney Diseases ,business ,Oxidative stress ,Biomarkers - Abstract
Liver-type fatty acid-binding protein (L-FABP) is a biomarker for the early detection of renal diseases in humans. L-FABP is a cytotoxic oxidation product secreted from the proximal tubules under ischemic and oxidative stress conditions. First, L-FABP gene expression in the kidney and liver was evaluated. Next, the urinary L-FABP concentrations in dogs with or without renal diseases were measured using a novel enzyme-linked immunosorbent assay kit. Urinary L-FABP was normalized relative to urinary creatinine (uCre) concentrations (µg/g uCre). Finally, the relationships between urinary L-FABP and renal biomarkers used in canine medicine or serum alanine transaminase (ALT) as an indicator of liver damage were examined. Serum and urine samples from 94 client-owned dogs including 23 dogs with renal diseases and 71 dogs without renal diseases were used for analysis. Relative L-FABP gene expression was confirmed both in the liver and kidney. Dogs with renal diseases had a significantly higher urinary L-FABP than those without, and its predictive cutoff value was 26 µg/g uCre. Urinary L-FABP was significantly correlated with serum creatinine (r=0.4674, P
- Published
- 2021
83. Morphometric evaluation of canine hepatocellular carcinoma using computed tomography: a promising tool for predicting malignancy
- Author
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Hiroshi Ohta, Rommaneeya Leela-Arporn, Noboru Sasaki, Genya Shimbo, and Mitsuyoshi Takiguchi
- Subjects
Multivariate analysis ,Carcinoma, Hepatocellular ,Canine Hepatocellular Carcinoma ,hepatic tumor ,Malignancy ,Sensitivity and Specificity ,Lesion ,Dogs ,medicine ,Internal Medicine ,Animals ,Dog Diseases ,Retrospective Studies ,Univariate analysis ,General Veterinary ,Full Paper ,business.industry ,Liver Neoplasms ,Area under the curve ,Odds ratio ,differentiation ,medicine.disease ,Confidence interval ,dog ,medicine.symptom ,business ,Nuclear medicine ,Tomography, X-Ray Computed ,morphometry - Abstract
The size of canine focal liver lesions (FLLs) is known to be one of the predicting criteria for malignancy. However, there are discrepancies for the measurement of maximum lesion size, resulting in contradicting results among studies and incidences of false positive outcomes. Thus far, the morphometric changes of FLLs for distinguishing malignancy from benignancy remains undocumented. This study aimed to investigate morphometric characteristics of FLLs using computed tomography (CT). CT images of 40 dogs with histopathological confirmation of 49 liver lesions, including 39 hepatocellular carcinomas and 10 nodular hyperplasias were retrospectively reviewed. The morphometric parameters including size (long and short axis diameters measured on transverse image), shape (measured by long to short axis (L/S) ratio), volume, and surface appearance of a liver lesion were evaluated using univariate and stepwise multivariate analyses, respectively. The results of univariate analysis showed that long and short axis diameters, L/S ratio, volume, and surface appearance of a lesion were significantly different between hepatocellular carcinomas and nodular hyperplasias. Multivariate analysis revealed that short axis diameter (>3.30 cm; odds ratio (OR): 36.1, 95% confidence interval (CI): 3.36-387.05, P=0.0031) and L/S ratio (>1.23; OR: 18.1, 95% CI: 1.61-205.12, P=0.0191) were independent predictors of malignancy, with the area under the curve of 0.9154. These results suggest that the combination of short axis diameter and L/S ratio is a promising tool for predicting liver malignancy with outstanding discriminating ability.
- Published
- 2021
84. Density assessment and reporting for Phlebotomus perniciosus and other sand fly species in periurban residential estates in Spain
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Pedro Pérez-Cutillas, J. Risueño, Tatiana Spitzova, P. F. Sánchez-López, Laura Murcia, L. J. Bernal, L. Del Rio, J.D. García-Martínez, Juana Ortiz, Carlos Martínez-Carrasco, Clara Serna Muñoz, P. De la Rúa, E. Goyena, Moisés Gonzálvez, R. Ruiz de Ybáñez, Francisco Collantes, S. Elshanat, and Eduardo Berriatua
- Subjects
Mediterranean climate ,Male ,Arthropods and Medical Entomology - Original Paper ,Density ,Distribution ,Environment ,Dogs ,Cave ,Canine leishmaniasis ,medicine ,Animals ,Phlebotomus ,Leishmania infantum ,Leishmaniasis ,Leishmania ,geography ,geography.geographical_feature_category ,General Veterinary ,biology ,Ecology ,General Medicine ,Vegetation ,medicine.disease ,biology.organism_classification ,Residential ,Insect Vectors ,Infectious Diseases ,Spain ,Insect Science ,Vector (epidemiology) ,Parasitology ,Female ,Psychodidae - Abstract
Green periurban residential areas in Mediterranean countries have flourished in the last decades and become foci for leishmaniasis. To remedy the absence of information on vector ecology in these environments, we examined phlebotomine sand fly distribution in 29 sites in Murcia City over a 3-year period, including the plots of 20 detached houses and nine non-urbanized sites nearby. We collected 5,066 specimens from five species using “sticky” interception and light attraction traps. The relative frequency of the main Leishmania infantum vector Phlebotomus perniciosus in these traps was 32% and 63%, respectively. Sand fly density was widely variable spatially and temporally and greatest in non-urbanized sites, particularly in caves and abandoned buildings close to domestic animal holdings. Phlebotomus perniciosus density in house plots was positively correlated with those in non-urbanized sites, greatest in larger properties with extensive vegetation and non-permanently lived, but not associated to dog presence or a history of canine leishmaniasis. Within house plots, sand fly density was highest in traps closest to walls. Furthermore, the study provides a guideline for insect density assessment and reporting and is envisioned as a building block towards the development of a pan-European database for robust investigation of environmental determinants of sand fly distribution.
- Published
- 2021
85. Comparative evaluation of three different formulas for predicting the parturition date of German Shepherds following somatic cell nuclear transfer
- Author
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Young-Bum Son, Yeon Woo Jeong, Yeon Ik Jeong, Mohammad Shamim Hossein, Woo Suk Hwang, Per Olof Olsson, and Gyeongmin Kim
- Subjects
General Veterinary ,Full Paper ,business.industry ,parturition date ,Parturition ,German Shepherd ,Gestational Age ,ultrasonography ,Chorion ,Biology ,Ultrasonography, Prenatal ,Comparative evaluation ,Theriogenoloy ,Andrology ,Dogs ,Fetus ,Pregnancy ,Somatic cell nuclear transfer ,Animals ,Female ,Ultrasonography ,dog cloning ,business - Abstract
Several studies have reported methods to estimate the parturition date of dogs using ultrasonographic measurements. However, these prediction models were mainly determined using ultrasonographic measurements of naturally pregnant small- and medium-sized dogs, and no such studies have been performed using dogs carrying cloned fetuses produced via somatic cell nuclear transfer. The present study evaluated the abilities of three reference formulas (Luvoni and Grioni, Milani et al., and Groppetti et al.), all of which were developed using data from naturally occurring pregnancies, to accurately predict the parturition date in surrogates carrying cloned German Shepherd (GS) fetuses. All three formulas were based on the use of inner chorionic cavity diameter (ICC) measurements, obtained via ultrasonography. For evaluation, a total of 54 ICC measurements were collected from 14 pregnant bitches carrying cloned GS fetuses. We found that the clinical accuracy of the breed-specific Groppetti et al. formula was highest among those of the three formulas tested, with 87% and 100% of the estimated parturition dates (calculated based on the ICC measurements) being within 1 and 2 days, respectively, of the actual delivery date. By contrast, the Luvoni and Grioni formula showed relatively low accuracy, and the Milani et al. formula showed higher accuracy than that reported previously for natural pregnancies.
- Published
- 2021
86. Age-dependent Radiation Dose Rates from Canine Sn-117m Treatments
- Author
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Matthew G. Arno and Chad Smith
- Subjects
dogs ,Epidemiology ,Health, Toxicology and Mutagenesis ,Age dependent ,Electrons ,Radiation Dosage ,Effective dose (radiation) ,symbols.namesake ,Internal conversion ,dose assessment ,Animals ,Radiology, Nuclear Medicine and imaging ,Monte Carlo ,Physics ,Auger effect ,business.industry ,Radiation dose ,Gamma ray ,Gamma Rays ,Total dose ,Papers ,symbols ,Nuclear medicine ,business ,Dose rate ,radiation protection - Abstract
Tin-117 m (Sn-117m) is used to treat dogs with osteoarthritic joints by radiosynoviorthesis. The decay process for Sn-117m is internal conversion wherein IC electrons and auger electrons provide the therapeutic effect. Additionally, the most prominent gamma emission is 158.6 keV. The effective dose rate received by a person interacting at close distances with a treated dog is needed to determine the person's total dose and thus regulatory compliance. Simple measurement of the dose rate at a given distance does not provide an accurate measurement of the effective dose to a person due to the non-uniform nature of the radiation field at close distances. MNCP models of the interactions of five ages of humans at three distances were created to determine the effective dose rates using the methodology from NRC Regulatory Guide 8.40. Ratios of the effective dose rate to the person to the measured dose rate at 1 m from the same source were calculated.
- Published
- 2021
87. Complications and outcomes associated with unilateral thyroidectomy in dogs with naturally occurring thyroid tumors: 156 cases (2003-2015).
- Author
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Reagan, Jennifer K., Selmic, Laura E., Fallon, Caroline, Sutton, Blake, Lafferty, Mary, Ben-Aderet, Daniel, Culp, William T. N., Liptak, Julius M., Duffy, Daniel, Simons, Micha, Boston, Sarah, and Lana, Sue
- Subjects
- *
THYROIDECTOMY , *DOGS , *ELECTRONIC paper , *HOSPITAL admission & discharge , *ASPIRATION pneumonia - Abstract
OBJECTIVE: To describe complications and outcomes of dogs undergoing unilateral thyroidectomy for the treatment of thyroid tumors. ANIMALS: 156 dogs undergoing unilateral thyroidectomy for a naturally occurring thyroid tumor. PROCEDURES: Dogs that underwent a unilateral thyroidectomy in 2003 through 2015 were included in a multi-institutional retrospective study. For each dog, information gathered through evaluation of electronic and paper records included perioperative complications, short-term outcome (survival to discharge from the hospital vs nonsurvival), and long-term outcome (survival time). RESULTS: In the perioperative period, complications occurred in 31 of the 156 (19.9%) dogs; hemorrhage was the most common intraoperative complication (12 [7.7%] dogs). Five of 156 (3.2%) dogs received a blood transfusion; these 5 dogs were among the 12 dogs that had hemorrhage listed as an intraoperative complication. Immediately after surgery, the most common complication was aspiration pneumonia (5 [3.2%] dogs). One hundred fifty-three of 156 (98.1%) dogs that underwent unilateral thyroidectomy survived to discharge from the hospital. One hundred-thirteen dogs were lost to follow-up; from the available data, the median survival time was 911 days (95% confidence interval, 704 to 1,466 days). CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that unilateral thyroidectomy in dogs with a naturally occurring thyroid tumor was associated with a perioperative mortality rate of 1.9% and a complication rate of 19.9% and that hemorrhage and aspiration pneumonia were the most common complications. Long-term survival of dogs undergoing unilateral thyroidectomy for the treatment of thyroid tumors was not uncommon. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
88. Do passive range of motion exercises lead to quicker postsurgical recovery of canine IVDD?
- Author
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Wallace, Alexander
- Subjects
RANGE of motion of joints ,SURGICAL decompression ,TREADMILL exercise ,MAST cell tumors ,INTERVERTEBRAL disk hernias ,HOME rehabilitation ,ELECTRIC stimulation ,INTERVERTEBRAL disk diseases ,SPINAL cord injuries - Abstract
This document provides a summary of several studies investigating the effects of rehabilitation on dogs recovering from intervertebral disc disease (IVDD) surgery. The studies examined the use of basic and intense rehabilitation techniques, including passive range of motion exercises, cold pack application, and various therapies. The findings suggest that basic rehabilitation alone did not significantly impact recovery time or rate. However, the combination of intense and basic rehabilitation was associated with a higher recovery rate. It is important to note that there is limited research specifically on basic rehabilitation techniques for postoperative IVDD patients, and further studies are needed to explore this area. The article discusses the effectiveness of different rehabilitation techniques for dogs recovering from intervertebral disc disease (IVDD) surgery. The study found that none of the papers reviewed reported increased rates of adverse effects. One study showed a lower complication rate in dogs that received a post-hospitalization rehabilitation program. However, there is contradictory evidence on the effectiveness of specific rehabilitation techniques. The article suggests that a multimodal approach to rehabilitation may be important for treating dogs with IVDD. The evidence is weak due to the lack of isolated studies on specific rehabilitation techniques. [Extracted from the article]
- Published
- 2023
- Full Text
- View/download PDF
89. Dynamic computed tomography evaluation of the nasopharynx in normal Beagle dogs
- Author
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Daji Noh, Youngwon Lee, Hojung Choi, Kija Lee, and Sooyoung Choi
- Subjects
Palate, Hard ,Computed tomography ,Beagle ,Bony labyrinth ,Dogs ,stomatognathic system ,Nasopharynx ,medicine ,Internal Medicine ,Animals ,Pterygoid hamulus ,collapsibility ,dynamic computed tomography ,General Veterinary ,medicine.diagnostic_test ,Soft palate ,Full Paper ,business.industry ,Skull ,Foramen lacerum ,Anatomy ,medicine.anatomical_structure ,dog ,Hard palate ,Dynamic ct ,Palate, Soft ,business ,Tomography, X-Ray Computed - Abstract
Pharyngeal collapsibility has been used as diagnostic criteria in dogs, whereas the normal range and quantitative method have not been studied. Dynamic and static computed tomography (CT) was performed in 23 normal Beagle dogs to quantify the nasopharyngeal collapsibility at different locations. Using dynamic CT, maximum and minimum nasopharyngeal cross-sectional areas (CSAs) were measured at the level of the cranial end of the soft palate, pterygoid hamulus, foramen lacerum, bony labyrinth, and caudal end of the soft palate. The ratio of all maximum and minimum CSA to nasopharyngeal CSA at the level of the caudal hard palate (rCSAmax and rCSAmin) and the nasopharyngeal collapsibility were calculated. The differences of rCSAmax, rCSAmin, and nasopharyngeal collapsibility were analyzed at various locations. The nasopharyngeal collapsibility at the level of foramen lacerum, bony labyrinth, and caudal end of soft palate were higher than the others. At the level of the caudal end of the soft palate, rCSAmin was lower than that of the foramen lacerum and bony labyrinth, whereas rCSAmax at foramen lacerum was higher than that of the caudal end of the soft palate. These results indicated that the nasopharynx at the level of foramen lacerum and caudal end of the soft palate were considered notable locations for evaluating collapsibility. Dynamic CT could show the nasopharyngeal dynamic profile and will be an adequate modality for evaluating nasopharynx. Our results will be helpful for further comparative studies in dogs with and without nasopharyngeal collapse.
- Published
- 2021
90. Molecular characterization of Leishmania species from stray dogs and human patients in Saudi Arabia
- Author
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Magda A. Ali, Abdullah D. Alanazi, Abdulazi S Alouffi S Alouffi, Abdulsadah A. Rahi, Marcos Antônio Bezerra-Santos, Jairo Alfonso Mendoza-Roldan, Fábio André Brayner, Hend H. A. M. Abdullah, Domenico Otranto, and Mohamed S Alyousif
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Leishmania tropica ,Patients ,030231 tropical medicine ,education ,Saudi Arabia ,Leishmaniasis, Cutaneous ,Polymerase Chain Reaction ,03 medical and health sciences ,0302 clinical medicine ,Medical microbiology ,Dogs ,Cutaneous leishmaniasis ,parasitic diseases ,medicine ,Animals ,Humans ,Leishmania major ,Leishmania species ,nPCR ,General Veterinary ,biology ,General Medicine ,medicine.disease ,biology.organism_classification ,Leishmania ,Virology ,030104 developmental biology ,Infectious Diseases ,Protozoology - Original Paper ,Insect Science ,kDNA ,Parasitology ,Nested polymerase chain reaction - Abstract
Leishmania major and Leishmania tropica cause cutaneous leishmaniasis in humans and dogs in several parts of the world, with a large number of cases recorded in the Middle East. However, when they occur in sympatry, the role of each species of Leishmania in the epidemiology of cutaneous leishmaniasis (CL) is not clear. To assess the frequency and to identify the species of Leishmania that infect humans and stray dogs in Riyadh and Al-Qaseem (Saudi Arabia), 311 stray dogs and 27 human patients who were suspected for Leishmania infection were examined for CL by a nested polymerase chain reaction (nPCR). Seven (25.9%) out of 27 human patients scored positive for Leishmania spp. (i.e., L. major in five patients from Riyadh and L. tropica in two patients from Al-Qaseem). Out of 311 dogs, five (1.6%) were infected by L. tropica. Data herein presented demonstrate the occurrence of L. tropica in dogs and humans in Saudi Arabia, as well as the occurrence of L. major in humans.
- Published
- 2021
91. Artists and Their Pets
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Stahl, Joan
- Published
- 1996
92. Quantitative mass spectrometry imaging of drugs and metabolites
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Bryn Flinders, Tiffany Porta Siegel, Darya Hadavi, Brent Viehmann, Rob J. Vreeken, Ron M. A. Heeren, Lieke Lamont, RS: M4I - Imaging Mass Spectrometry (IMS), and Imaging Mass Spectrometry (IMS)
- Subjects
Accuracy and precision ,MSI comparison ,01 natural sciences ,Biochemistry ,Mass spectrometry imaging ,Mass Spectrometry ,Analytical Chemistry ,MRM based drug imaging ,03 medical and health sciences ,Dogs ,Absolute quantification ,Pharmacokinetics ,Limit of Detection ,Animals ,Desorption electrospray ionization ,030304 developmental biology ,Detection limit ,0303 health sciences ,Chromatography ,Chemistry ,010401 analytical chemistry ,Selected reaction monitoring ,0104 chemical sciences ,Triple quadrupole mass spectrometer ,Liver ,Pharmaceutical Preparations ,Mimetic tissue model ,Large animal ,Research Paper - Abstract
Mass spectrometry imaging (MSI) provides insight into the molecular distribution of a broad range of compounds and, therefore, is frequently applied in the pharmaceutical industry. Pharmacokinetic and toxicological studies deploy MSI to localize potential drugs and their metabolites in biological tissues but currently require other analytical tools to quantify these pharmaceutical compounds in the same tissues. Quantitative mass spectrometry imaging (Q-MSI) is a field with challenges due to the high biological variability in samples combined with the limited sample cleanup and separation strategies available prior to MSI. In consequence, more selectivity in MSI instruments is required. This can be provided by multiple reaction monitoring (MRM) which uses specific precursor ion-product ion transitions. This targeted approach is in particular suitable for pharmaceutical compounds because their molecular identity is known prior to analysis. In this work, we compared different analytical platforms to assess the performance of MRM detection compared to other MS instruments/MS modes used in a Q-MSI workflow for two drug candidates (A and B). Limit of detection (LOD), linearity, and precision and accuracy of high and low quality control (QC) samples were compared between MS instruments/modes. MRM mode on a triple quadrupole mass spectrometer (QqQ) provided the best overall performance with the following results for compounds A and B: LOD 35.5 and 2.5 μg/g tissue, R2 0.97 and 0.98 linearity, relative standard deviation QC R2 0.86–0.98 and 0.86–0.98 linearity, relative standard deviation QC Graphical abstract
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- 2021
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93. What’s the point? Domestic dogs’ sensitivity to the accuracy of human informants
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Angie M. Johnston, Sarah MacKay Marton, Julia Espinosa, Madeline Pelgrim, Emma C. Tecwyn, and Daphna Buchsbaum
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0106 biological sciences ,Comparative cognition ,Applied psychology ,Behavioural sciences ,Experimental and Cognitive Psychology ,Trust ,010603 evolutionary biology ,01 natural sciences ,Dogs ,Social cognition ,Animals ,Humans ,0501 psychology and cognitive sciences ,050102 behavioral science & comparative psychology ,Ecology, Evolution, Behavior and Systematics ,Probability ,Original Paper ,Point (typography) ,Gestures ,Psychological research ,05 social sciences ,Recognition, Psychology ,Social learning ,Social Learning ,Test (assessment) ,Psychology ,Gesture ,Canine cognition - Abstract
Dogs excel at understanding human social-communicative gestures like points and can distinguish between human informants who vary in characteristics such as knowledge or familiarity. This study explores if dogs, like human children, can use human social informants’ past accuracy when deciding whom to trust. Experiment 1 tested whether dogs would behave differently in the presence of an accurate (vs. inaccurate) informant. Dogs followed an accurate informant’s point significantly above chance. Further, when presented with an inaccurate point, dogs were more likely to ignore it and choose the correct location. Experiment 2 tested whether dogs could use informant past accuracy to selectively follow the point of the previously accurate informant. In test trials when informants simultaneously pointed at different locations (only one of which contained a treat), dogs chose the accurate informant at chance levels. Experiment 3 controlled for non-social task demands (e.g. understanding of hidden baiting and occlusion events) that may have influenced Experiment 2 performance. In test trials, dogs chose to follow the accurate (vs. inaccurate) informant. This suggests that like children, dogs may be able to use informants’ past accuracy when choosing between information sources. Supplementary Information The online version contains supplementary material available at 10.1007/s10071-021-01493-5.
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- 2021
94. Satisfaction and satisfaction affecting problem behavior in different types of adopted dogs
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Francesca Bertomoro, Omar Bonetti, and Simona Normando
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Animal behavior ,Behavioral complaint ,Galgo ,Online questionnaire survey ,Sighthound ,Problem Behavior ,General Veterinary ,Demographics ,Full Paper ,Separation (statistics) ,Human-Animal Bond ,Ethology ,Personal Satisfaction ,animal behavior ,behavioral complaint ,Dogs ,online questionnaire survey ,Surveys and Questionnaires ,galgo ,Animals ,sighthound ,Psychology ,Clinical psychology - Abstract
Many dogs are relinquished worldwide, so it is important to enhance adoptions' success. We aimed at investigating factors associated with owners' satisfaction with adopted dogs, both in general and focusing on galgos. Data on 392 dogs (191 galgos) were gathered using an online survey, investigating dogs' and owners' demographics, satisfaction with the adopted dog and post-adoption behavior. Satisfaction was affected by different variables in galgos' owners as compared to non-sighthound non-podenco dogs' ones, with only the presence of disobedience on walks negatively affecting satisfaction in both samples. Depending on dogs' type, the presence of some behavioral problems was associated with decreased satisfaction with the dog (e.g., destructiveness for galgos, or separation problems for non-sighthound non-podenco dogs), whereas that of others increased it (e.g., not being interested in social interactions with dogs for galgos, and shadowing for non-sighthound non-podenco dogs). The variables most often being predictors of the behaviors influencing satisfaction were dog type, with being a galgo as a negative predictor, and dog's age, with being older as a negative predictor. Further studies on dog adopters' satisfaction are needed.
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- 2021
95. Efficacy and safety of allogenic canine adipose tissue-derived mesenchymal stem cell therapy for insulin-dependent diabetes mellitus in four dogs: A pilot study
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Woo-Jin Song, Ju-Hyun An, Jung-Hwa Lee, Jin-Ok Ahn, Sung-Yong Rhew, Hyung-Kyu Chae, Qiang Li, Hwa-Young Youn, and Su-Min Park
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medicine.medical_specialty ,endocrine system diseases ,040301 veterinary sciences ,Seoul ,medicine.medical_treatment ,Adipose tissue ,Pilot Projects ,Mesenchymal Stem Cell Transplantation ,Gastroenterology ,0403 veterinary science ,03 medical and health sciences ,chemistry.chemical_compound ,Dogs ,Polyuria ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,Medicine ,hyperlipidemia ,Animals ,Dog Diseases ,mesenchymal stem cell ,030304 developmental biology ,0303 health sciences ,General Veterinary ,Full Paper ,business.industry ,C-peptide ,Insulin ,Mesenchymal Stem Cells ,04 agricultural and veterinary sciences ,medicine.disease ,Transplantation ,Fructosamine ,Diabetes Mellitus, Type 1 ,chemistry ,Adipose Tissue ,diabetes mellitus ,dog ,medicine.symptom ,business ,Polydipsia - Abstract
Mesenchymal stem cells (MSCs) possess regenerative and immunomodulatory properties and can control the immune dysregulation that leads to β-cell destruction. Stem-cell transplantation could thus manage insulin-dependent diabetes mellitus (IDDM) in dogs. In this pilot study, we aimed to assess canine adipose tissue-derived MSCs (cAT-MSCs) transplantation as a treatment for canine diabetes mellitus. This study included four dogs with over a year of insulin treatment for IDDM, following diagnosis at the Veterinary Medicine Teaching Hospital of Seoul National University. Allogenic cAT-MSCs were infused intravenously three or five times monthly to dogs with IDDM. Blood and urine samples were obtained monthly. General clinical symptoms, including changes in body weight, vitality, appetite, and water intake were assessed. Three of the four owners observed improvement of vitality after stem cell treatment. Two of the four dogs showed improvement in appetite and body weight, polyuria, and polydipsia. C-peptide has increased by about 5-15% in three of the cases, and fructosamine and HbA1c levels have improved in two of the cases. Hyperlipidemia was resolved in two of the dogs, and there was no concurrent bacterial cystitis in any of the dogs. C-peptide secretion and lipid metabolism are associated with diabetic complications. Improvement in these parameters following the treatment suggests that cAT-MSC transplantation in dogs with IDDM might help to improve their insulin secretory capacity and prevent diabetic complications.
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- 2021
96. Every dog has its day: An in-depth analysis of the creative ability of visual generative AI.
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HEDBLOM, MARIA M.
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GENERATIVE artificial intelligence ,CREATIVE ability ,DOGS ,ART theory ,ARTIFICIAL intelligence - Abstract
The recent remarkable success of generative AI models to create text and images has already started altering our perspective of intelligence and the "uniqueness" of humanity in this world. Simultaneously, arguments on why AI will never exceed human intelligence are ever-present as seen in Landgrebe and Smith (2022). To address whether machines may rule the world after all, this paper zooms in on one of the aspects of intelligence Landgrebe and Smith (2022) neglected to consider: creativity. Using Rhodes four Ps of creativity as a starting point, this paper evaluates the creative ability in visual generative AI models with respect to the state of the art in creativity theory. The most part of the reflective evaluation is performed through a case study in generating illustrations of dogs using the generative AI tool Midjourney. [ABSTRACT FROM AUTHOR]
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- 2024
97. You Are Not Welcome! A Media Analysis of Risk Factors, Prevalence and Management of Free-Roaming Dogs in Iran.
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Amiraslani, Farshad
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HUMAN-animal relationships ,FACTOR analysis ,RISK assessment ,DOMESTICATION of animals ,ANIMAL behavior ,DOGS ,URBAN agriculture - Abstract
Simple Summary: An increase in the number of free-roaming dogs and the related consequences (e.g., dog biting) has been observed across Iranian cities. So far, no clear scientific reasons for this increase were demonstrated, and a few existing domestic studies have only investigated the behaviours and frequencies of these animals in the cities. Based on online news articles, the on-the-ground reasons, challenges and dog-controlling activities mentioned by key urban and health authorities are examined in this paper. A scientific link is established between increased urbanisation, its features and its implications for dogs and humans. History has witnessed a long-term relationship between humans and animals. Historical documents and modern findings prove that humans' needs to use animals for companions or services are commonplace in many parts of the world, leading to the domestication of certain animals. Yet, modern societies have degraded many natural habitats for wildlife, confining them to small patches of landscapes or urban areas. Whether a domesticated/free-roaming animal or a wild species, their close contact with humans can create cumbersome situations for both species. This paper explores a link between online media content and on-the-ground efforts to manage free-roaming dogs as a rare case study. As indicated by news articles, the municipal costs of managing free-roaming dogs in Iranian cities have increased, and this can potentially derail the control of such dogs in the long run. This paper lays out pivotal factors for recent increasing human–animal encounters, which have led to many challenges (e.g., rabies) across cities in Iran. We show that some urban features (e.g., topography) can influence the presence and behaviours of free-roaming animals in the cities. The findings of this paper can be related to other developing countries where the plague of rabies is rising. [ABSTRACT FROM AUTHOR]
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- 2023
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98. An Assessment of Scientific Evidence Relating to the Effect of Early Experience on the Risk of Human-Directed Aggression by Adult Dogs.
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Baslington-Davies, Ann, Howell, Helen, Hogue, Todd E., and Mills, Daniel S.
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ANIMAL aggression ,HUMAN-animal relationships ,SCIENTIFIC literature ,DOGS ,AGRICULTURE ,TECHNICAL reports ,SEX ratio - Abstract
Simple Summary: Dogs that bite or show aggression towards humans are a worldwide problem. Whilst many dog–human relationships are valuable and bring much happiness, when the relationship breaks down and results in an aggressive encounter, the consequences are, at best, unpleasant for all those involved. It is commonly believed that the early experiences of dogs may contribute to their behaviour as adults. We reviewed and synthesised the scientific evidence surrounding this belief in order to establish what it tells us about the importance of early life experiences with regard to minimising the possibility of dogs developing aggressive responses to humans as adult animals. We found evidence in support of the potential importance of the following risk factors: the source of the animal, the age at which it was rehomed, the reason for the acquisition, the experience level of the owner, the animal's socialisation experiences, the consistent husbandry and management practices, the training, the sex ratio of the litter and the history of dogs that display aggression in the pedigree. Human-directed aggression by domestic dogs is a major worldwide public health problem. The causes of aggression are complex, and research in this area often has to balance ecological validity with pragmatic controls; accordingly, it often does not meet the thresholds for quality typically used in reviews applying a classical "evidence-based" approach. Here, we propose a method of literature assessment that makes the "best use" of available evidence to identify and synthesise evidence relating to the most likely risk factors reported in the scientific literature. We used a systematic review process to initially identify relevant literature relating to potential early life experience (i.e., in the first six months of life) risk factors in the dog for human-directed aggression in the adult animal. Fourteen papers met our initial screening process and were subsequently analysed in detail, with data extracted and effect sizes calculated where possible. This highlighted the potential importance of the source of the animal, the age at which it was rehomed, the reason for the acquisition, the experience level of the owner, the animal's socialisation experiences, the consistent husbandry and management practices, the training, the sex ratio of the litter and the history of dogs that display aggression in the pedigree as risk factors. Taken together, it seems that early experiences which limit the ability to develop effective coping strategies and routines may be particularly important. We provide guidance for the future standardised reporting of risk related to human-directed aggression by dogs to allow greater synthesis of the literature in the future. [ABSTRACT FROM AUTHOR]
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- 2023
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99. Deep learning–based MR‐to‐CT synthesis: The influence of varying gradient echo–based MR images as input channels
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René M. Castelein, Matteo Maspero, Max A. Viergever, Peter R. Seevinck, Cornelis A. T. van den Berg, Marijn van Stralen, Harrie Weinans, Mateusz C. Florkow, Frank Zijlstra, Koen Willemsen, and Linda G W Kerkmeijer
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Mean squared error ,gradient echo ,Full Papers—Computer Processing and Modeling ,Dogs ,Similarity (network science) ,Robustness (computer science) ,Hounsfield scale ,Journal Article ,Image Processing, Computer-Assisted ,Animals ,Humans ,Radiology, Nuclear Medicine and imaging ,Mathematics ,MR contrasts ,Artificial neural network ,Full Paper ,business.industry ,Deep learning ,deep learning ,Ranging ,Pattern recognition ,Repeatability ,Magnetic Resonance Imaging ,Radiology Nuclear Medicine and imaging ,synthetic CT ,Artificial intelligence ,Neural Networks, Computer ,business ,Tomography, X-Ray Computed ,Rare cancers Radboud Institute for Health Sciences [Radboudumc 9] - Abstract
Contains fulltext : 219835.pdf (Publisher’s version ) (Open Access) PURPOSE: To study the influence of gradient echo-based contrasts as input channels to a 3D patch-based neural network trained for synthetic CT (sCT) generation in canine and human populations. METHODS: Magnetic resonance images and CT scans of human and canine pelvic regions were acquired and paired using nonrigid registration. Magnitude MR images and Dixon reconstructed water, fat, in-phase and opposed-phase images were obtained from a single T1 -weighted multi-echo gradient-echo acquisition. From this set, 6 input configurations were defined, each containing 1 to 4 MR images regarded as input channels. For each configuration, a UNet-derived deep learning model was trained for synthetic CT generation. Reconstructed Hounsfield unit maps were evaluated with peak SNR, mean absolute error, and mean error. Dice similarity coefficient and surface distance maps assessed the geometric fidelity of bones. Repeatability was estimated by replicating the training up to 10 times. RESULTS: Seventeen canines and 23 human subjects were included in the study. Performance and repeatability of single-channel models were dependent on the TE-related water-fat interference with variations of up to 17% in mean absolute error, and variations of up to 28% specifically in bones. Repeatability, Dice similarity coefficient, and mean absolute error were statistically significantly better in multichannel models with mean absolute error ranging from 33 to 40 Hounsfield units in humans and from 35 to 47 Hounsfield units in canines. CONCLUSION: Significant differences in performance and robustness of deep learning models for synthetic CT generation were observed depending on the input. In-phase images outperformed opposed-phase images, and Dixon reconstructed multichannel inputs outperformed single-channel inputs.
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- 2019
100. Characterization of a novel nicotinamide adenine dinucleotide-cytochrome b5 reductase mutation associated with canine hereditary methemoglobinemia
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Reeko Sato, Yayoi Otsuka-Yamasaki, Haruka Shino, Osamu Inanami, and Masahiro Yamasaki
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Nonsynonymous substitution ,040301 veterinary sciences ,CYB5R3 ,Reductase ,Nicotinamide adenine dinucleotide ,Methemoglobinemia ,Cat Diseases ,Biochemistry ,0403 veterinary science ,03 medical and health sciences ,chemistry.chemical_compound ,Dogs ,medicine ,Missense mutation ,Animals ,Dog Diseases ,Cytochrome b5 reductase ,030304 developmental biology ,0303 health sciences ,General Veterinary ,Nicotinamide ,Full Paper ,Chemistry ,missense mutation ,nicotinamide adenine dinucleotide-cytochrome b5 reductase ,04 agricultural and veterinary sciences ,hereditary methemoglobinemia ,medicine.disease ,NAD ,Molecular biology ,Cytochromes b5 ,Mutation ,dog ,Cats ,Cytochrome-B(5) Reductase - Abstract
Hereditary methemoglobinemia associated with nicotinamide adenine dinucleotide-cytochrome b5 reductase (b5R) deficiency is a rare autosomal recessive disorder in animals. Recently, nonsynonymous b5R gene (CYB5R3) variants have been reported to be associated with canine and feline hereditary methemoglobinemia. However, the underlying molecular mechanisms of canine and feline methemoglobinemia caused by these nonsynonymous variants have not yet been reported. Previously, we reported a Pomeranian dog family with hereditary methemoglobinemia, carrying CYB5R3 mutation of an A>C transition at codon 194 in exon 7, replacing an isoleucine residue with leucine (p.Ile194Leu). In this study, we investigated the enzymatic and structural properties of the soluble form of wild-type and Ile194Leu canine b5Rs to characterize the effects of this missense mutation. Our results showed that the kinetic properties of the mutant enzyme were not affected by this amino acid substitution. The secondary structure of the wild-type and Ile194Leu b5Rs detected by circular dichroism showed a similar pattern. However, the mutant enzyme exhibited decreased heat stability and increased susceptibility to trypsin hydrolysis. Moreover, the thermostability and unfolding measurements indicated that the mutant enzyme was more sensitive to temperature-dependent denaturation than the wild-type b5R. We concluded from these results that unstable mutant enzyme properties with normal enzymatic activity would be associated with hereditary methemoglobinemia in the Pomeranian dog family.
- Published
- 2021
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