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Main observation and conclusion: Accurate determination of acetaminophen concentration is essential for studying the metabolic status of acetaminophen in clinical practice. In this study, nickel phosphide was used for electrochemical detection of acetaminophen for the first time. An electrochemical acetaminophen sensor based on Ni2P nanosheets was successfully constructed and the sensor showed many convincing properties: (a) a good linear range (0.5 μmol/L—4.5 mmol/L); (b) a moderate sensitivity (131.1 μA·mmol–1·L·cm–2); (c) a low detection limit (0.107 μmol/L). In addition, the sensor also showed excellent selectivity, robust stability and reliable repeatability. Further experiments demonstrate that the prepared sensor can be used for quantitative detection of acetaminophen in commercial medical drugs. [ABSTRACT FROM AUTHOR]

Colorectal cancer (CRC) remains a significant contributor to the global mortality rate, and a single biomarker cannot meet the specificity required for CRC screening. To this end, we developed a multiplexed, pump-free surface-enhanced Raman scattering (SERS) microfluidic chip (LoC-SERS) using a one-step recognition release mechanism; the aptamer-functionalized novel Au nanocrown array (AuNCA) was used as the detection element embedded in the detection zone of the platform for rapid and specific detection of protein markers in multiple samples simultaneously. Here, the corresponding aptamer specifically captured the protein marker, causing the complementary strand of the aptamer carrying the Raman signal molecule to be shed, reducing the SERS signal. Based on this platform, sensitive and specific detection of the target can be accomplished within 15 min with detection limits of 0.031 pg/mL (hnRNP A1) and 0.057 pg/mL (S100P). Meanwhile, the platform was consistent with ELISA results when used to test clinical. By substituting different aptamers, this platform can provide a new solution for the rapid and sensitive detection of protein markers, which has promising applications in future disease detection. [Display omitted] • A POCT platform was developed for rapid and sensitive detection of protein markers. • The prepared AuNCA has good SERS signal amplification effect. • The platform can complete the detection of a target in less than 15 min. • The platform has a promising application in clinical testing. [ABSTRACT FROM AUTHOR]

In this communication, for the first time, an easy preparation method of nickel–iron (Ni–Fe) hybrid nanocubes for glucose oxidation, which is highly efficient in alkaline media, is described. As an impressive redox probe for glucose, the Ni–Fe hybrid nanocubes exhibit many remarkable sensing performances, for example, low-response time (7 s), broad detection range (from 10 μM to 20.5 mM), and low detection limit (0.64 μM, S/N = 3). The selectivity, reliability and stability of the Ni–Fe hybrid nanocubes for glucose oxidation are also investigated. The glucose sensor based on Ni–Fe hybrid nanocubes shows a superior sensing performance to that of Ni-based or Fe-based electrochemical sensors. Furthermore, the Ni–Fe hybrid nanocubes were used to detect glucose in real samples of human blood serum. All the findings demonstrate that the glucose sensor based on Ni–Fe hybrid nanocubes is successful for glucose detection and shows promise for future applications in medical diagnostics, the food industry and biological processes. [ABSTRACT FROM AUTHOR]

Surface-enhanced Raman scattering (SERS) based on the double-antibody sandwich format is reported for the determination of carcinoembryonic antigen. Ordered gold nanobowl arrays were fabricated and conjugated with anticarcinoembryonic as capturing substrates, and gold nanoshells, adsorbed with 4-mercaptobenzonic acid, were modified with anticarcinoembryonic antigen as labeling tags. After the carcinoembryonic antigen was captured on ordered gold nanobowl arrays, the labeling tags were bonded to the captured carcinoembryonic antigen. The interaction of SERS substrates (ordered gold nanobowl arrays) and SERS labels (gold nanoshells) showed high sensitivity and a low detection limit for carcinoembryonic antigen. The linear dynamic range of SERS for carcinoembryonic antigen was from 5 pg/mL to 100 ng/mL with a linear relationship between carcinoembryonic antigen concentration and SERS intensity. The detection limit was 1.73 pg/mL. SERS detection may be used for other cancer biomarkers and provides potential for the clinical diagnosis of cancer biomarkers. [ABSTRACT FROM PUBLISHER]

A sensor for the determination of guanine (G) and hydrogen peroxide (H 2 O 2) is developed based on Ni–Fe Prussian blue analogues hollow nanocubes (Ni–Fe PBA HNCs) for the first time. As a remarkable redox probe towards G and H 2 O 2 oxidation, Ni–Fe PBA HNCs exhibit a series of predominant sensing performances as follows: lower limit of detection, broader linear range and higher selectivity due to the homogeneous hollow structure, high specific surface and the enhanced electron transfer ability of Ni–Fe PBA HNCs. As a G sensor, it exhibits a wide linear range (0.05–4.0 mM) and a low detection limit of 0.0104 μM (S/N = 3). As a H 2 O 2 sensor, the Ni–Fe PBA HNCs show superior sensing performances with a low detection limit of 0.291 μM (S/N = 3) and a wide detection range of 0.1–20 mM. By cause of these advantages, the real-time detection of G and H 2 O 2 in human saliva are triumphantly accomplishment, indicating the applicability of Ni–Fe PBA HNCs. Image 1 • A sensor for the determination of guanine and hydrogen peroxide is developed based on Ni-Fe Prussian blue analogues hollow nanocubes for the first time. • As a remarkable redox probe towards guanine and hydrogen peroxide oxidation, the Ni-Fe hollow nanocubes exhibit a series of predominant sensing performances. • The real-time detection of H 2 O 2 in human saliva is triumphantly accomplishment, indicating the applicability of Ni-Fe hollow nanocubes. [ABSTRACT FROM AUTHOR]