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Deficit irrigation is a new strategy to increase water use efficiency of cotton in arid areas, but it is not clear if it interacts with plant density. The objective of this study was to determine the effects of deficit irrigation and plant density as well as their interaction on the growth, yield and fiber quality of irrigated cotton. Two field experiments were conducted at three sites in 2013 and one site from 2014 to 2015 in an arid area of Xinjiang. A randomized complete block design with three replicates was used to determine the effects of 6 irrigation regimes on seedcotton yield in the first experiment, while a split-plot design was used in the second experiment with the main plots assigned to irrigation regime (saturation, regular and deficit) and the subplots to plant density (high, medium and low) to examine cotton yield, fiber quality and water productivity as affected by plant density under deficit irrigation. Averaged across the three sites, drip irrigation ranging from 3650 to 4700 m 3 /ha did not significantly affect cotton yield, but seedcotton yield under 3650 m 3 /ha in S1 was 6.3% lower than that under 4000 m 3 /ha. Thus, it is quite appropriate to regularly drip-irrigate at 4000 m 3 /ha in the experimental area. Deficit irrigation at high plant density also maintained a relatively higher leaf area index (LAI) and net assimilation rate (NAR), particularly at late stages of plant growth and development, than saturation or regular irrigation. Plant density ranging from 18 to 24 plants/m 2 produced more seedcotton than 12 plants/m 2 under regular irrigation. Increasing irrigation to saturation levels had little effects on cotton yield regardless of plant density; saturation irrigation at high plant density even reduced cotton yield compared with regular irrigation at medium plant density. Under deficit irrigation, the high plant density produced 9.1-17% greater yield and 9.3-16.8% higher irrigation water productivity (IWP) than low or medium plant density, and comparable yield to medium or high plant density under regular irrigation. This high yield under deficit irrigation at high plant density was due to increased plant biomass occasioned by high plant population and improved harvest index. Deficit irrigation did not affect fiber quality in 2014, but reduced fiber length and increased fiber micronaire value in 2015. Conclusively, use of high plant density under deficit irrigation can be a promising alternative for water saving without compromising cotton yield under arid conditions. [ABSTRACT FROM AUTHOR]

Fiber quality is a major breeding goal in cotton, but phenotypically direct selection is often hindered. In this study, we identified fiber quality and yield related loci using GWAS based on 2.97 million SNPs obtained from 10.65× resequencing data of 1081 accessions. The results showed that 585 novel fiber loci, including two novel stable SNP peaks associated with fiber length on chromosomes At12 and Dt05 and one novel genome regions linked with fiber strength on chromosome Dt12 were identified. Furthermore, by means of gene expression analysis, GhM_A12G0090 , GhM_D05G1692 , GhM_D12G3135 were identified and GhM_D11G2208 function was identified in Arabidopsis. Additionally, 14 consistent and stable superior haplotypes were identified, and 25 accessions were detected as possessing these 14 superior haplotype in breeding. This study providing fundamental insight relevant to identification of genes associated with fiber quality and yield will enhance future efforts toward improvement of upland cotton. • A total of 909 novel fiber and yield loci were detected via WGRS with 10.65× coverage depth in 1,081 cotton accessions. • Three novel stable SNP peaks associated with FL and FS were found. • GhM_D11G2208 can promote cell elongation based up leaf trichome and dark-grown hypocotyls in Arabidopsis. • 14 consistent and stable superior haplotypes were detected based on 915 SNPs in Dt11 region. [ABSTRACT FROM AUTHOR]

Relay intercropping of garlic with full-season cotton is currently one of the dominant cropping systems in China, but the net benefit is decreasing because the system is labor-intensive. Direct planting of short-season cotton after garlic harvest may increase net revenue through reducing labor and material input. Three field experiments were consecutively conducted in Jinxiang County of China, to determine the effects of plant density and soil fertility on yield, yield components, boll load and leaf senescence in the 1st and 2nd experiments. In the third experiment, we compared the economic benefits of the two cropping systems. Data from the 1st experiment showed that plant density affected yield and yield components, with the optimum plant density being 3.0 plants m −2 for full-season cotton and 9.0 plants m −2 for short-season cotton. In the 2nd experiment, the seedcotton yield of full-season cotton was 9.1% higher under high than medium soil fertility, but there was no yield difference between the two soil fertility levels for short-season cotton. Full-season cotton exhibited larger boll load, earlier leaf senescence and lower boll weight under medium than high fertility. Results of the third experiment showed that seedcotton yield or output value of short-season cotton was 14.5% lower than that of full-season cotton, but the gross return for short-season cotton was 69.2% higher than that for full-season cotton because the short-season cotton required 27.3% less labor and material inputs. The overall results showed that late planted short-season cotton after garlic harvest can be a promising alternative for enhancing the benefits of garlic-cotton production in China. [ABSTRACT FROM AUTHOR]

Abstract: As the most important cultural practices for cotton production, the single effects of plant density and [nitrogen (N) and potassium (K)] fertilization on yield and yield components are well documented but their combined effects on Bt cotton are poorly understood. Using a split–split plot design with four replications, we conducted a two-year field experiment in two fields, one with lower fertility and the other with higher fertility, in the Yellow River Valley of China. The aim was to evaluate both the individual and combined effects of plant density and nitrogen and potassium fertilization on yield, yield components and uptake of major nutrients. The main plots were assigned to plant density (4.5 and 7.5plants/m2), while nitrogen (0 and 240kgN/ha) and potassium fertilization (0 and 150kgK/ha) were assigned to the sub- and sub–subplots. Lint yield was improved with high plant density (7.5plants/m2) in the lower fertility field, particularly without N and K application, but not in the higher fertility field. Nitrogen or K application also increased lint yield, and a combination of high plant density, N and K application further improved lint yield in the lower fertility field, while only K application increased lint yield in the higher fertility field. Lint percentage was not affected by any of the variables studied. Thus, the yield increase due to plant density, fertilization or their combinations was attributed to increases in boll number or boll weight. The ratio of seed cotton to stalk (RSS) was linearly correlated with harvest index, and thus can be a simple indicator of dry matter allocation to reproductive structures. Increased yield due to plant density and fertilization was mainly attributed to the enhanced biological yield in the lower fertility field, while the yield increase due to K fertilization was mainly due to increased RSS in the higher fertility field. The plants used approximately equal N and P to produce 100kg lint in both fields, but the uptake of K to produce 100kg lint in the higher fertility field was about 21% more than that in the lower fertility field. Ratios of N:P:K were 1:0.159:0.604 in the lower fertility field and 1:0.159:0.734 in higher fertility field. This study suggests that K fertilization was extremely important for maintaining high yield, although luxury consumption occurred in the higher fertility field; N was applied more than required in the highly fertile field, and increased plant density would be beneficial to cotton yield in the lower fertility field. [Copyright &y& Elsevier]

Manual removal of the main-stem growth tip is traditionally used to break the apical dominance of cotton (Gossypium hirsutum L.). Chemical topping with plant growth regulators also effectively inhibits apical dominance. However, the effect of chemical topping on yield increases and whether plant density or ecological conditions affect its efficacy are unclear. Therefore, a three-year field experiment with a split-plot design was conducted to determine the effects of plant topping, plant density, and their interactions on cotton yield and related physiological and agronomical parameters at three sites with different ecological conditions in China. In each site, the main plots were assigned low, moderate, or high plant density and the subplots were assigned no topping, manual topping, or chemical topping. Growth, yield, yield components, earliness, and late-season leaf photosynthesis as well as labor and material inputs were examined each year. Compared with no topping, both chemical and manual topping greatly reduced plant height at all sites. Manual topping increased seed cotton yield and earliness in all tested plant densities and sites. However, plant density but not ecological condition greatly mediated the effect of chemical topping on yield. At low plant density, the yields with chemical topping were 4–6% lower than those with no topping and 5.5–10.8% lower than those with manual topping at the three sites. Although yields with chemical topping were comparable with those of manual topping at moderate and high plant densities, they were 8.6–12.8% higher at moderate density and 13.8–16.4% higher at high plant density than those with no topping across years and sites. Averaged across the sites, chemical topping reduced biological yield by 12.7% at low plant density. Although biological yield decreased slightly, chemical and manual topping increased the harvest index by 12.4% and 13.3% at moderate density and by 15.6% and 17.4% at high density, respectively. In comparison with no topping, the reduction in seed cotton yield with chemical topping at low plant density was attributed to insufficient biological yield, whereas the increase in yield at moderate and high plant densities was mainly due to greater partitioning of assimilates to reproductive tissues. Compared with manual topping, chemical topping produced 23.2% lower net returns as a result of lower seed cotton yield at low plant density but produced 8.1% and 20.9% higher net returns at moderate and high plant densities, respectively, because of savings in labor inputs and comparable seed cotton yields. In addition, chemical topping increased the earliness percentage compared with that of no topping. Overall, this study demonstrates that chemical topping is a promising alternative to traditional manual topping under moderate or high cotton plant density. • Manual topping increased cotton yield regardless of plant densities and ecological conditions relative to non-topping. • Chemical topping decreased yield at low plant density relative to non-topping because of greater biomass reduction. • Chemical topping increased cotton yield at moderate and high plant densities via greater assimilates partitioning to fruits. • Chemical topping could replace manual topping at moderate and high plant densities without ecological dependence. [ABSTRACT FROM AUTHOR]

Abstract: Cotton is usually managed with a normal planting production system (NPPS) that involves planting in mid-April at a moderate plant density (4.5plants/m2) in the Yellow River Valley of China, but drought or cold stress in spring often delays cotton planting, and results in reduced yield and maturity at this plant density. Two experiments were conducted for 4 consecutive years, to test if yield and fiber quality can be maintained or improved by increased plant density for relatively late-planted cotton. Results in the first experiment in 2001 and 2002 showed that average lint yields were not significantly affected by plant density (3.0, 4.5, 6.0 or 7.5plants/m2) or by planting date (mid-April or early May), but significant interactions between planting date and plant density on lint yield were detected in both years. Normal planted cotton at a plant density of 3.0–4.5plants/m2, and late-planted cotton at 7.5plants/m2 produced higher lint yield than other planting date and density combinations. Experiment 2 compared NPPS with a late planting production system (LPPS) which involves planting in early May at 7.5plants/m2 over 2 years. The NPPS and LPPS had similar lint yields in both years. Cotton plants in both systems produced approximately 75% of total lint in the first two harvests, indicating no significantly delayed earliness in LPPS relative to NPPS. Fiber from late-season bolls exhibited reduced strength and micronaire in both systems, but there were no significant differences in fiber properties for early- and mid-season fiber between the two systems. In terms of green leaf area index and leaf chlorophyll content, leaf senescence or premature senescence of cotton plants was considerably alleviated by either altered source to sink balance or the uptake of K in LPPS compared to NPPS. It was concluded that the LPPS, a relatively late-planted cotton production system with increased plant density under intensive field management, might be a potential alternative for growing cotton. [Copyright &y& Elsevier]

• Non-mulched short-season cotton produced less fiber but greater returns than mulched full-season cotton as it consumed less inputs. • Non-mulched short-season cotton delayed leaf senescence due to reduced boll load and produced comparable cotton yields to the mulched. • Non-mulched short-season cotton reduced the residual film pollution, thus offering an alternative to mulched full-season cotton. The early planting of full-season cotton (Gossypium hirsutum L.) at a moderate plant density under plastic mulching is a traditional means of growing cotton in the Yellow River valley of China. However, it is labor intensive, consumes a large amount of materials, and also results in residual plastic pollution in the soil. While our previous studies indicated that late planted short-season cotton under plastic mulching greatly reduces labor and material inputs and increases net revenue, the residual plastic pollution remains an issue. It is unclear if short-season cotton can be managed without yield loss in the absence of plastic mulching. A field experiment was therefore conducted using a random complete block design with four treatments, including the early planting of full-season cotton with (FCM) and without plastic mulching (FC), and the late planting of short-season cotton with (SCM) and without plastic mulching (SC), in the Yellow River valley from 2017 to 2019. The yield, yield components, input and output values, earliness, dynamics of flowering and boll setting, and late-season leaf senescence in cotton were examined. The results showed that early planted full-season cotton under mulching (FCM) produced a 21.1 and 20.4 % higher seedcotton yield and boll density, respectively, and improved earliness over the non-mulched treatment, while the boll weight did not differ significantly. Short-season cotton without plastic mulching produced comparable seedcotton yields to that with mulching (SCM), and there was no significant difference in average boll weight, boll density, or earliness between the mulching and non-mulching treatments. However, SC had a 7.0 and 12.1 % smaller boll density and boll weight, respectively, than FCM and thus produced a 17.9 % lower seedcotton yield. Therefore, SC produced 14.9 and 14.4 % greater net returns than SCM and FCM, respectively, because it consumed less labor and material inputs. Short-season cotton without plastic mulching delayed leaf senescence due to reduced boll load and thus increased boll weight in the late season compared with SCM. In addition, SC exhibited improved earliness as a result of a higher flowering rate, faster fruiting, and more concentrated boll opening than FCM. Short-season cotton without plastic mulching also avoided the residual film pollution in the soil. The overall results demonstrated that the late planting of short-season cotton without mulching is a promising alternative for sustainable cotton production in the Yellow River valley region of China as well as other cotton growing areas with similar ecology. [ABSTRACT FROM AUTHOR]