1. Oligoethylenimine-grafted chitosan as enhanced T1 contrast agent for in vivo targeted tumor MRI.
- Author
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Tong X, Liu M, Zhang K, Cao Y, Dong J, Jiang B, Lu B, Zheng H, Zhang H, and Pei R
- Subjects
- Animals, Aziridines chemistry, Cell Line, Tumor, Contrast Media chemical synthesis, Folic Acid chemistry, Gadolinium DTPA chemistry, Image Enhancement methods, Mice, Mice, Nude, Molecular Probe Techniques, Neoplasms, Experimental pathology, Reproducibility of Results, Sensitivity and Specificity, Chitosan chemistry, Folate Receptors, GPI-Anchored metabolism, Folic Acid pharmacokinetics, Gadolinium DTPA pharmacokinetics, Magnetic Resonance Imaging methods, Neoplasms, Experimental diagnostic imaging, Neoplasms, Experimental metabolism
- Abstract
Purpose: To synthesize and characterize an effective macromolecular magnetic resonance imaging (MRI) contrast agent based on oligoethylenimine-grafted chitosan with targeting capability., Materials and Methods: In this study we synthesized and characterized oligoethylenimine-grafted chitosan copolymers, followed by conjugating with Gd-DTPA and folic acid. The toxicity was evaluated by WST assay, and in vitro MRI studies were performed in comparison with Gd-DTPA. Finally, the contrast enhancement of the new macromolecular MRI contrast agent was then evaluated in the mice bearing KB xenografts., Results: Compared to Gd-DTPA (4.3 mM(-1) s(-1) ), this macromolecular contrast agent (mCA) exhibited much higher T1 relaxivity (14.4 mM(-1) s(-1) ), up to 3.3 times higher. Meanwhile, the WST assay illustrated that the viability of KB cells remained at 90% even when the Gd concentration was 1 mM. During the in vivo study, the image contrast produced by FA-mCA was higher than one produced by mCA, up to 2.5 times higher., Conclusion: Our results showed this macromolecular contrast agent has potential for developing sensitive and biocompatible MRI probe with targeting capability. J. Magn. Reson. Imaging 2016;44:23-29., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
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