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1. Two-step stacking in capillary zone electrophoresis featuring sweeping and micelle to solvent stacking: I. Organic cations

Author

Quirino, Joselito P.

Subjects
*CAPILLARY electrophoresis, *MICELLES, *ORGANIC solvents, *CATIONS, *HYDRODYNAMICS, *SODIUM dodecyl sulfate, *SULFATES, *SOLUTION (Chemistry), *ADRENERGIC beta blockers, *ANTIDEPRESSANTS, *COMPARATIVE studies
Abstract

Abstract: Two-step stacking of organic cations by sweeping and micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE) is presented. The simple procedure involves hydrodynamic injection of a micellar sodium dodecyl sulfate solution before the sample that is prepared without the micelles. The micelles sweep and transport the cations to the boundary zone between the sample and CZE buffer. The presence of organic solvent in the CZE buffer induces the second stacking step of MSS. The LODs obtained for the four beta blocker and two tricyclic antidepressant test drugs were 20–50 times better compared to typical injection. [ABSTRACT FROM AUTHOR]

Published
2010
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