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2. Comparative biology and life tables of sorghum aphid Melanaphis sorghi (Theobald) (Hemiptera: Aphididae) from Mexico, at different temperatures
- Author
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Peña-Martínez, Rebeca, Lomeli-Flores, J. Refugio, Bujanos-Muñiz, Rafael, Salas-Monzón, Raquel, Hernández-Torres, Oscar Eduardo, Marín-Jarillo, Antonio, Ibarra, Jorge E., Vanegas-Rico, Juan Manuel, and Muñoz-Viveros, Ana Lilia
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- 2024
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3. A first update on mapping the human genetic architecture of COVID-19
- Author
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COVID-19 Host Genetics Initiative, Pathak, GA, Karjalainen, J, Stevens, C, Neale, BM, Daly, M, Ganna, A, Andrews, SJ, Kanai, M, Cordioli, M, Polimanti, R, Harerimana, N, Pirinen, M, Liao, RG, Chwialkowska, K, Trankiem, A, Balaconis, MK, Nguyen, H, Solomonson, M, Veerapen, K, Wolford, B, Roberts, G, Park, D, Ball, CA, Coignet, M, McCurdy, S, Knight, S, Partha, R, Rhead, B, Zhang, M, Berkowitz, N, Gaddis, M, Noto, K, Ruiz, L, Pavlovic, M, Hong, EL, Rand, K, Girshick, A, Guturu, H, Baltzell, AH, Niemi, MEK, Rahmouni, S, Guntz, J, Beguin, Y, Pigazzini, S, Nkambule, L, Georges, M, Moutschen, M, Misset, B, Darcis, G, Guiot, J, Azarzar, S, Gofflot, S, Claassen, S, Malaise, O, Huynen, P, Meuris, C, Thys, M, Jacques, J, Leonard, P, Frippiat, F, Giot, J-B, Sauvage, A-S, Frenckell, CV, Belhaj, Y, Lambermont, B, Nakanishi, T, Morrison, DR, Mooser, V, Richards, JB, Butler-Laporte, G, Forgetta, V, Li, R, Ghosh, B, Laurent, L, Belisle, A, Henry, D, Abdullah, T, Adeleye, O, Mamlouk, N, Kimchi, N, Afrasiabi, Z, Rezk, N, Vulesevic, B, Bouab, M, Guzman, C, Petitjean, L, Tselios, C, Xue, X, Afilalo, J, Afilalo, M, Oliveira, M, Brenner, B, Brassard, N, Durand, M, Schurr, E, Lepage, P, Ragoussis, J, Auld, D, Chassé, M, Kaufmann, DE, Lathrop, GM, Adra, D, Hayward, C, Glessner, JT, Shaw, DM, Campbell, A, Morris, M, Hakonarson, H, Porteous, DJ, Below, J, Richmond, A, Chang, X, Polikowski, H, Lauren, PE, Chen, H-H, Wanying, Z, Fawns-Ritchie, C, North, K, McCormick, JB, Glessner, JR, Gignoux, CR, Wicks, SJ, Crooks, K, Barnes, KC, Daya, M, Shortt, J, Rafaels, N, Chavan, S, Timmers, PRHJ, Wilson, JF, Tenesa, A, Kerr, SM, D’Mellow, K, Shahin, D, El-Sherbiny, YM, von Hohenstaufen, KA, Sobh, A, Eltoukhy, MM, Nkambul, L, Elhadidy, TA, Abd Elghafar, MS, El-Jawhari, JJ, Mohamed, AAS, Elnagdy, MH, Samir, A, Abdel-Aziz, M, Khafaga, WT, El-Lawaty, WM, Torky, MS, El-shanshory, MR, Yassen, AM, Hegazy, MAF, Okasha, K, Eid, MA, Moahmed, HS, Medina-Gomez, C, Ikram, MA, Uitterlinden, AG, Mägi, R, Milani, L, Metspalu, A, Laisk, T, Läll, K, Lepamets, M, Esko, T, Reimann, E, Naaber, P, Laane, E, Pesukova, J, Peterson, P, Kisand, K, Tabri, J, Allos, R, Hensen, K, Starkopf, J, Ringmets, I, Tamm, A, Kallaste, A, Alavere, H, Metsalu, K, Puusepp, M, Batini, C, Tobin, MD, Venn, LD, Lee, PH, Shrine, N, Williams, AT, Guyatt, AL, John, C, Packer, RJ, Ali, A, Free, RC, Wang, X, Wain, LV, Hollox, EJ, Bee, CE, Adams, EL, Palotie, A, Ripatti, S, Ruotsalainen, S, Kristiansson, K, Koskelainen, S, Perola, M, Donner, K, Kivinen, K, Kaunisto, M, Rivolta, C, Bochud, P-Y, Bibert, S, Boillat, N, Nussle, SG, Albrich, W, Quinodoz, M, Kamdar, D, Suh, N, Neofytos, D, Erard, V, Voide, C, Friolet, R, Vollenweider, P, Pagani, JL, Oddo, M, zu Bentrup, FM, Conen, A, Clerc, O, Marchetti, O, Guillet, A, Guyat-Jacques, C, Foucras, S, Rime, M, Chassot, J, Jaquet, M, Viollet, RM, Lannepoudenx, Y, Portopena, L, Bochud, PY, Desgranges, F, Filippidis, P, Guéry, B, Haefliger, D, Kampouri, EE, Manuel, O, Munting, A, Papadimitriou-Olivgeris, M, Regina, J, Rochat-Stettler, L, Suttels, V, Tadini, E, Tschopp, J, Van Singer, M, Viala, B, Boillat-Blanco, N, Brahier, T, Hügli, O, Meuwly, JY, Pantet, O, Gonseth Nussle, S, Bochud, M, D’Acremont, V, Estoppey Younes, S, Albrich, WC, Cerny, A, O’Mahony, L, von Mering, C, Frischknecht, M, Kleger, G-R., Filipovic, M, Kahlert, CR, Wozniak, H, Negro, TR, Pugin, J, Bouras, K, Knapp, C, Egger, T, Perret, A, Montillier, P, di Bartolomeo, C, Barda, B, de Cid, R, Carreras, A, Moreno, V, Kogevinas, M, Galván-Femenía, I, Blay, N, Farré, X, Sumoy, L, Cortés, B, Mercader, JM, Guindo-Martinez, M, Torrents, D, Garcia-Aymerich, J, Castaño-Vinyals, G, Dobaño, C, Gori, M, Renieri, A, Mari, F, Mondelli, MU, Castelli, F, Vaghi, M, Rusconi, S, Montagnani, F, Bargagli, E, Franchi, F, Mazzei, MA, Cantarini, L, Tacconi, D, Feri, M, Scala, R, Spargi, G, Nencioni, C, Bandini, M, Caldarelli, GP, Canaccini, A, Ognibene, A, D’Arminio Monforte, A, Girardis, M, Antinori, A, Francisci, D, Schiaroli, E, Scotton, PG, Panese, S, Scaggiante, R, Monica, MD, Capasso, M, Fiorentino, G, Castori, M, Aucella, F, Biagio, AD, Masucci, L, Valente, S, Mandalà, M, Zucchi, P, Giannattasio, F, Coviello, DA, Mussini, C, Tavecchia, L, Crotti, L, Rizzi, M, Rovere, MTL, Sarzi-Braga, S, Bussotti, M, Ravaglia, S, Artuso, R, Perrella, A, Romani, D, Bergomi, P, Catena, E, Vincenti, A, Ferri, C, Grassi, D, Pessina, G, Tumbarello, M, Pietro, MD, Sabrina, R, Luchi, S, Furini, S, Dei, S, Benetti, E, Picchiotti, N, Sanarico, M, Ceri, S, Pinoli, P, Raimondi, F, Biscarini, F, Stella, A, Zguro, K, Capitani, K, Tanfoni, M, Fallerini, C, Daga, S, Baldassarri, M, Fava, F, Frullanti, E, Valentino, F, Doddato, G, Giliberti, A, Tita, R, Amitrano, S, Bruttini, M, Croci, S, Meloni, I, Mencarelli, MA, Rizzo, CL, Pinto, AM, Beligni, G, Tommasi, A, Sarno, LD, Palmieri, M, Carriero, ML, Alaverdian, D, Busani, S, Bruno, R, Vecchia, M, Belli, MA, Mantovani, S, Ludovisi, S, Quiros-Roldan, E, Antoni, MD, Zanella, I, Siano, M, Emiliozzi, A, Fabbiani, M, Rossetti, B, Bergantini, L, D’Alessandro, M, Cameli, P, Bennett, D, Anedda, F, Marcantonio, S, Scolletta, S, Guerrini, S, Conticini, E, Frediani, B, Spertilli, C, Donati, A, Guidelli, L, Corridi, M, Croci, L, Piacentini, P, Desanctis, E, Cappelli, S, Verzuri, A, Anemoli, V, Pancrazzi, A, Lorubbio, M, Miraglia, FG, Venturelli, S, Cossarizza, A, Vergori, A, Gabrieli, A, Riva, A, Paciosi, F, Andretta, F, Gatti, F, Parisi, SG, Baratti, S, Piscopo, C, Russo, R, Andolfo, I, Iolascon, A, Carella, M, Merla, G, Squeo, GM, Raggi, P, Marciano, C, Perna, R, Bassetti, M, Sanguinetti, M, Giorli, A, Salerni, L, Parravicini, P, Menatti, E, Trotta, T, Coiro, G, Lena, F, Martinelli, E, Mancarella, S, Gabbi, C, Maggiolo, F, Ripamonti, D, Bachetti, T, Suardi, C, Parati, G, Bottà, G, Domenico, PD, Rancan, I, Bianchi, F, Colombo, R, Barbieri, C, Acquilini, D, Andreucci, E, Segala, FV, Tiseo, G, Falcone, M, Lista, M, Poscente, M, Vivo, OD, Petrocelli, P, Guarnaccia, A, Baroni, S, van Heel, DA, Hunt, KA, Trembath, RC, Huang, QQ, Martin, HC, Mason, D, Trivedi, B, Wright, J, Finer, S, Akhtar, S, Anwar, M, Arciero, E, Ashraf, S, Breen, G, Chung, R, Curtis, CJ, Chowdhury, M, Colligan, G, Deloukas, P, Durham, C, Griffiths, C, Hurles, M, Hussain, S, Islam, K, Khan, A, Lavery, C, Lee, SH, Lerner, R, MacArthur, D, MacLaughlin, B, Martin, H, Miah, S, Newman, B, Safa, N, Tahmasebi, F, Griffiths, CJ, Smith, AV, Boughton, AP, Li, KW, LeFaive, J, Annis, A, Niavarani, A, Aliannejad, R, Sharififard, B, Amirsavadkouhi, A, Naderpour, Z, Tadi, HA, Aleagha, AE, Ahmadi, S, Moghaddam, SBM, Adamsara, A, Saeedi, M, Abdollahi, H, Hosseini, A, Chariyavilaskul, P, Jantarabenjakul, W, Hirankarn, N, Chamnanphon, M, Suttichet, TB, Shotelersuk, V, Pongpanich, M, Phokaew, C, Chetruengchai, W, Putchareon, O, Torvorapanit, P, Puthanakit, T, Suchartlikitwong, P, Nilaratanakul, V, Sodsai, P, Brumpton, BM, Hveem, K, Willer, C, Zhou, W, Rogne, T, Solligard, E, Åsvold, BO, Franke, L, Boezen, M, Deelen, P, Claringbould, A, Lopera, E, Warmerdam, R, Vonk, JM, van Blokland, I, Lanting, P, Ori, APS, Feng, Y-CA, Mercader, J, Weiss, ST, Karlson, EW, Smoller, JW, Murphy, SN, Meigs, JB, Woolley, AE, Green, RC, Perez, EF, Zöllner, S, Wang, J, Beck, A, Sloofman, LG, Ascolillo, S, Sebra, RP, Collins, BL, Levy, T, Buxbaum, JD, Sealfon, SC, Jordan, DM, Thompson, RC, Gettler, K, Chaudhary, K, Belbin, GM, Preuss, M, Hoggart, C, Choi, S, Underwood, SJ, Salib, I, Britvan, B, Keller, K, Tang, L, Peruggia, M, Hiester, LL, Niblo, K, Aksentijevich, A, Labkowsky, A, Karp, A, Zlatopolsky, M, Zyndorf, M, Charney, AW, Beckmann, ND, Schadt, EE, Abul-Husn, NS, Cho, JH, Itan, Y, Kenny, EE, Loos, RJF, Nadkarni, GN, Do, R, O’Reilly, P, Huckins, LM, Ferreira, MAR, Abecasis, GR, Leader, JB, Cantor, MN, Justice, AE, Carey, DJ, Chittoor, G, Josyula, NS, Kosmicki, JA, Horowitz, JE, Baras, A, Gass, MC, Yadav, A, Mirshahi, T, Hottenga, JJ, Bartels, M, de geus, EEJC, Nivard, MMG, Verma, A, Ritchie, MD, Rader, D, Li, B, Verma, SS, Lucas, A, Bradford, Y, Abedalthagafi, M, Alaamery, M, Alshareef, A, Sawaji, M, Massadeh, S, AlMalik, A, Alqahtani, S, Baraka, D, Harthi, FA, Alsolm, E, Safieh, LA, Alowayn, AM, Alqubaishi, F, Mutairi, AA, Mangul, S, Almutairi, M, Aljawini, N, Albesher, N, Arabi, YM, Mahmoud, ES, Khattab, AK, Halawani, RT, Alahmadey, ZZ, Albakri, JK, Felemban, WA, Suliman, BA, Hasanato, R, Al-Awdah, L, Alghamdi, J, AlZahrani, D, AlJohani, S, Al-Afghani, H, AlDhawi, N, AlBardis, H, Alkwai, S, Alswailm, M, Almalki, F, Albeladi, M, Almohammed, I, Barhoush, E, Albader, A, Alotaibi, S, Alghamdi, B, Jung, J, fawzy, MS, Alrashed, M, Zeberg, H, Frithiof, R, Hultström, M, Lipcsey, M, Tardif, N, Rooyackers, O, Grip, J, Maricic, T, Helgeland, Ø, Magnus, P, Trogstad, L-IS, Lee, Y, Harris, JR, Mangino, M, Spector, TD, Emma, D, Moutsianas, L, Caulfield, MJ, Scott, RH, Kousathanas, A, Pasko, D, Walker, S, Stuckey, A, Odhams, CA, Rhodes, D, Fowler, T, Rendon, A, Chan, G, Arumugam, P, Karczewski, KJ, Martin, AR, Wilson, DJ, Spencer, CCA, Crook, DW, Wyllie, DH, O’Connell, AM, Atkinson, EG, Tsuo, K, Baya, N, Turley, P, Gupta, R, Walters, RK, Palmer, DS, Sarma, G, Cheng, N, Lu, W, Churchhouse, C, Goldstein, JI, King, D, Seed, C, Daly, MJ, Finucane, H, Bryant, S, Satterstrom, FK, Band, G, Earle, SG, Lin, S-K, Arning, N, Koelling, N, Armstrong, J, Rudkin, JK, Callier, S, Cusick, C, Soranzo, N, Zhao, JH, Danesh, J, Angelantonio, ED, Butterworth, AS, Sun, YV, Huffman, JE, Cho, K, O’Donnell, CJ, Tsao, P, Gaziano, JM, Peloso, G, Ho, Y-L, Smieszek, SP, Polymeropoulos, C, Polymeropoulos, V, Polymeropoulos, MH, Przychodzen, BP, Fernandez-Cadenas, I, Planas, AM, Perez-Tur, J, Llucià-Carol, L, Cullell, N, Muiño, E, Cárcel-Márquez, J, DeDiego, ML, Iglesias, LL, Soriano, A, Rico, V, Agüero, D, Bedini, JL, Lozano, F, Domingo, C, Robles, V, Ruiz-Jaén, F, Márquez, L, Gomez, J, Coto, E, Albaiceta, GM, García-Clemente, M, Dalmau, D, Arranz, MJ, Dietl, B, Serra-Llovich, A, Soler, P, Colobrán, R, Martín-Nalda, A, Martínez, AP, Bernardo, D, Rojo, S, Fiz-López, A, Arribas, E, de la Cal-Sabater, P, Segura, T, González-Villa, E, Serrano-Heras, G, Martí-Fàbregas, J, Jiménez-Xarrié, E, de Felipe Mimbrera, A, Masjuan, J, García-Madrona, S, Domínguez-Mayoral, A, Villalonga, JM, Menéndez-Valladares, P, Chasman, DI, Sesso, HD, Manson, JE, Buring, JE, Ridker, PM, Franco, G, Davis, L, Lee, S, Priest, J, Sankaran, VG, van Heel, D, Biesecker, L, Kerchberger, VE, Baillie, JK, Pathak, Gita A., Karjalainen, Juha, Stevens, Christine, Neale, Benjamin M., Daly, Mark, Ganna, Andrea, Andrews, Shea J., Kanai, Masahiro, Cordioli, Mattia, Polimanti, Renato, Harerimana, Nadia, Pirinen, Matti, Liao, Rachel G., Chwialkowska, Karolina, Trankiem, Amy, Balaconis, Mary K., Nguyen, Huy, Solomonson, Matthew, Veerapen, Kumar, Wolford, Brooke, Roberts, Genevieve, Park, Danny, Ball, Catherine A., Coignet, Marie, McCurdy, Shannon, Knight, Spencer, Partha, Raghavendran, Rhead, Brooke, Zhang, Miao, Berkowitz, Nathan, Gaddis, Michael, Noto, Keith, Ruiz, Luong, Pavlovic, Milo, Hong, Eurie L., Rand, Kristin, Girshick, Ahna, Guturu, Harendra, Baltzell, Asher Haug, Niemi, Mari E. K., Rahmouni, Souad, Guntz, Julien, Beguin, Yve, Pigazzini, Sara, Nkambule, Lindokuhle, Georges, Michel, Moutschen, Michel, Misset, Benoit, Darcis, Gille, Guiot, Julien, Azarzar, Samira, Gofflot, Stéphanie, Claassen, Sabine, Malaise, Olivier, Huynen, Pascale, Meuris, Christelle, Thys, Marie, Jacques, Jessica, Léonard, Philippe, Frippiat, Frederic, Giot, Jean-Baptiste, Sauvage, Anne-Sophie, Frenckell, Christian Von, Belhaj, Yasmine, Lambermont, Bernard, Nakanishi, Tomoko, Morrison, David R., Mooser, Vincent, Richards, J. Brent, Butler-Laporte, Guillaume, Forgetta, Vincenzo, Li, Rui, Ghosh, Biswarup, Laurent, Laetitia, Belisle, Alexandre, Henry, Danielle, Abdullah, Tala, Adeleye, Olumide, Mamlouk, Noor, Kimchi, Nofar, Afrasiabi, Zaman, Rezk, Nardin, Vulesevic, Branka, Bouab, Meriem, Guzman, Charlotte, Petitjean, Loui, Tselios, Chri, Xue, Xiaoqing, Afilalo, Jonathan, Afilalo, Marc, Oliveira, Maureen, Brenner, Bluma, Brassard, Nathalie, Durand, Madeleine, Schurr, Erwin, Lepage, Pierre, Ragoussis, Jianni, Auld, Daniel, Chassé, Michaël, Kaufmann, Daniel E., Lathrop, G. Mark, Adra, Darin, Hayward, Caroline, Glessner, Joseph T., Shaw, Douglas M., Campbell, Archie, Morris, Marcela, Hakonarson, Hakon, Porteous, David J., Below, Jennifer, Richmond, Anne, Chang, Xiao, Polikowski, Hannah, Lauren, Petty E., Chen, Hung-Hsin, Wanying, Zhu, Fawns-Ritchie, Chloe, North, Kari, McCormick, Joseph B., Glessner, Joseph R., Gignoux, Christopher R., Wicks, Stephen J., Crooks, Kristy, Barnes, Kathleen C., Daya, Michelle, Shortt, Jonathan, Rafaels, Nichola, Chavan, Sameer, Timmers, Paul R. H. J., Wilson, James F., Tenesa, Albert, Kerr, Shona M., D’Mellow, Kenton, Shahin, Doaa, El-Sherbiny, Yasser M., von Hohenstaufen, Kathrin Aprile, Sobh, Ali, Eltoukhy, Madonna M., Nkambul, Lindokuhle, Elhadidy, Tamer A., Abd Elghafar, Mohamed S., El-Jawhari, Jehan J., Mohamed, Attia A. S., Elnagdy, Marwa H., Samir, Amr, Abdel-Aziz, Mahmoud, Khafaga, Walid T., El-Lawaty, Walaa M., Torky, Mohamed S., El-shanshory, Mohamed R., Yassen, Amr M., Hegazy, Mohamed A. F., Okasha, Kamal, Eid, Mohammed A., Moahmed, Hanteera S., Medina-Gomez, Carolina, Ikram, M. Arfan, Uitterlinden, Andre G., Mägi, Reedik, Milani, Lili, Metspalu, Andre, Laisk, Triin, Läll, Kristi, Lepamets, Maarja, Esko, Tõnu, Reimann, Ene, Naaber, Paul, Laane, Edward, Pesukova, Jaana, Peterson, Pärt, Kisand, Kai, Tabri, Jekaterina, Allos, Raili, Hensen, Kati, Starkopf, Joel, Ringmets, Inge, Tamm, Anu, Kallaste, Anne, Alavere, Helene, Metsalu, Kristjan, Puusepp, Mairo, Batini, Chiara, Tobin, Martin D., Venn, Laura D., Lee, Paul H., Shrine, Nick, Williams, Alexander T., Guyatt, Anna L., John, Catherine, Packer, Richard J., Ali, Altaf, Free, Robert C., Wang, Xueyang, Wain, Louise V., Hollox, Edward J., Bee, Catherine E., Adams, Emma L., Palotie, Aarno, Ripatti, Samuli, Ruotsalainen, Sanni, Kristiansson, Kati, Koskelainen, Sami, Perola, Marku, Donner, Kati, Kivinen, Katja, Kaunisto, Mari, Rivolta, Carlo, Bochud, Pierre-Yve, Bibert, Stéphanie, Boillat, Noémie, Nussle, Semira Gonseth, Albrich, Werner, Quinodoz, Mathieu, Kamdar, Dhryata, Suh, Noémie, Neofytos, Dionysio, Erard, Véronique, Voide, Cathy, Friolet, R., Vollenweider, P., Pagani, J. L., Oddo, M., zu Bentrup, F. Meyer, Conen, A., Clerc, O., Marchetti, O., Guillet, A., Guyat-Jacques, C., Foucras, S., Rime, M., Chassot, J., Jaquet, M., Viollet, R. Merlet, Lannepoudenx, Y., Portopena, L., Bochud, P. Y., Desgranges, F., Filippidis, P., Guéry, B., Haefliger, D., Kampouri, E. E., Manuel, O., Munting, A., Papadimitriou-Olivgeris, M., Regina, J., Rochat-Stettler, L., Suttels, V., Tadini, E., Tschopp, J., Van Singer, M., Viala, B., Boillat-Blanco, N., Brahier, T., Hügli, O., Meuwly, J. Y., Pantet, O., Gonseth Nussle, S., Bochud, M., D’Acremont, V., Estoppey Younes, S., Albrich, W. C., Suh, N., Cerny, A., O’Mahony, L., von Mering, C., Frischknecht, M., Kleger, G-R., Filipovic, M., Kahlert, C. R., Wozniak, H., Negro, T. Rochat, Pugin, J., Bouras, K., Knapp, C., Egger, T., Perret, A., Montillier, P., di Bartolomeo, C., Barda, B., de Cid, Rafael, Carreras, Anna, Moreno, Victor, Kogevinas, Manoli, Galván-Femenía, Iván, Blay, Natalia, Farré, Xavier, Sumoy, Lauro, Cortés, Beatriz, Mercader, Josep Maria, Guindo-Martinez, Marta, Torrents, David, Garcia-Aymerich, Judith, Castaño-Vinyals, Gemma, Dobaño, Carlota, Gori, Marco, Renieri, Alessandra, Mari, Francesca, Mondelli, Mario Umberto, Castelli, Francesco, Vaghi, Massimo, Rusconi, Stefano, Montagnani, Francesca, Bargagli, Elena, Franchi, Federico, Mazzei, Maria Antonietta, Cantarini, Luca, Tacconi, Danilo, Feri, Marco, Scala, Raffaele, Spargi, Genni, Nencioni, Cesira, Bandini, Maria, Caldarelli, Gian Piero, Canaccini, Anna, Ognibene, Agostino, D’Arminio Monforte, Antonella, Girardis, Massimo, Antinori, Andrea, Francisci, Daniela, Schiaroli, Elisabetta, Scotton, Pier Giorgio, Panese, Sandro, Scaggiante, Renzo, Monica, Matteo Della, Capasso, Mario, Fiorentino, Giuseppe, Castori, Marco, Aucella, Filippo, Biagio, Antonio Di, Masucci, Luca, Valente, Serafina, Mandalà, Marco, Zucchi, Patrizia, Giannattasio, Ferdinando, Coviello, Domenico A., Mussini, Cristina, Tavecchia, Luisa, Crotti, Lia, Rizzi, Marco, Rovere, Maria Teresa La, Sarzi-Braga, Simona, Bussotti, Maurizio, Ravaglia, Sabrina, Artuso, Rosangela, Perrella, Antonio, Romani, Davide, Bergomi, Paola, Catena, Emanuele, Vincenti, Antonella, Ferri, Claudio, Grassi, Davide, Pessina, Gloria, Tumbarello, Mario, Pietro, Massimo Di, Sabrina, Ravaglia, Luchi, Sauro, Furini, Simone, Dei, Simona, Benetti, Elisa, Picchiotti, Nicola, Sanarico, Maurizio, Ceri, Stefano, Pinoli, Pietro, Raimondi, Francesco, Biscarini, Filippo, Stella, Alessandra, Zguro, Kristina, Capitani, Katia, Tanfoni, Marco, Fallerini, Chiara, Daga, Sergio, Baldassarri, Margherita, Fava, Francesca, Frullanti, Elisa, Valentino, Floriana, Doddato, Gabriella, Giliberti, Annarita, Tita, Rossella, Amitrano, Sara, Bruttini, Mirella, Croci, Susanna, Meloni, Ilaria, Mencarelli, Maria Antonietta, Rizzo, Caterina Lo, Pinto, Anna Maria, Beligni, Giada, Tommasi, Andrea, Sarno, Laura Di, Palmieri, Maria, Carriero, Miriam Lucia, Alaverdian, Diana, Busani, Stefano, Bruno, Raffaele, Vecchia, Marco, Belli, Mary Ann, Mantovani, Stefania, Ludovisi, Serena, Quiros-Roldan, Eugenia, Antoni, Melania Degli, Zanella, Isabella, Siano, Matteo, Emiliozzi, Arianna, Fabbiani, Massimiliano, Rossetti, Barbara, Bergantini, Laura, D’Alessandro, Miriana, Cameli, Paolo, Bennett, David, Anedda, Federico, Marcantonio, Simona, Scolletta, Sabino, Guerrini, Susanna, Conticini, Edoardo, Frediani, Bruno, Spertilli, Chiara, Donati, Alice, Guidelli, Luca, Corridi, Marta, Croci, Leonardo, Piacentini, Paolo, Desanctis, Elena, Cappelli, Silvia, Verzuri, Agnese, Anemoli, Valentina, Pancrazzi, Alessandro, Lorubbio, Maria, Miraglia, Federica Gaia, Venturelli, Sophie, Cossarizza, Andrea, Vergori, Alessandra, Gabrieli, Arianna, Riva, Agostino, Paciosi, Francesco, Andretta, Francesca, Gatti, Francesca, Parisi, Saverio Giuseppe, Baratti, Stefano, Piscopo, Carmelo, Russo, Roberta, Andolfo, Immacolata, Iolascon, Achille, Carella, Massimo, Merla, Giuseppe, Squeo, Gabriella Maria, Raggi, Pamela, Marciano, Carmen, Perna, Rita, Bassetti, Matteo, Sanguinetti, Maurizio, Giorli, Alessia, Salerni, Lorenzo, Parravicini, Pierpaolo, Menatti, Elisabetta, Trotta, Tullio, Coiro, Gabriella, Lena, Fabio, Martinelli, Enrico, Mancarella, Sandro, Gabbi, Chiara, Maggiolo, Franco, Ripamonti, Diego, Bachetti, Tiziana, Suardi, Claudia, Parati, Gianfranco, Bottà, Giordano, Domenico, Paolo Di, Rancan, Ilaria, Bianchi, Francesco, Colombo, Riccardo, Barbieri, Chiara, Acquilini, Donatella, Andreucci, Elena, Segala, Francesco Vladimiro, Tiseo, Giusy, Falcone, Marco, Lista, Mirjam, Poscente, Monica, Vivo, Oreste De, Petrocelli, Paola, Guarnaccia, Alessandra, Baroni, Silvia, van Heel, David A., Hunt, Karen A., Trembath, Richard C., Huang, Qin Qin, Martin, Hilary C., Mason, Dan, Trivedi, Bhavi, Wright, John, Finer, Sarah, Akhtar, Shaheen, Anwar, Mohammad, Arciero, Elena, Ashraf, Samina, Breen, Gerome, Chung, Raymond, Curtis, Charles J., Chowdhury, Maharun, Colligan, Grainne, Deloukas, Pano, Durham, Ceri, Griffiths, Chri, Hurles, Matt, Hussain, Shapna, Islam, Kamrul, Khan, Ahsan, Khan, Amara, Lavery, Cath, Lee, Sang Hyuck, Lerner, Robin, MacArthur, Daniel, MacLaughlin, Bev, Martin, Hilary, Miah, Shefa, Newman, Bill, Safa, Nishat, Tahmasebi, Farah, Griffiths, Christopher J., Smith, Albert V., Boughton, Andrew P., Li, Kevin W., LeFaive, Jonathon, Annis, Aubrey, Niavarani, Ahmadreza, Aliannejad, Rasoul, Sharififard, Bahareh, Amirsavadkouhi, Ali, Naderpour, Zeinab, Tadi, Hengameh Ansari, Aleagha, Afshar Etemadi, Ahmadi, Saeideh, Moghaddam, Seyed Behrooz Mohseni, Adamsara, Alireza, Saeedi, Morteza, Abdollahi, Hamed, Hosseini, Abdolmajid, Chariyavilaskul, Pajaree, Jantarabenjakul, Watsamon, Hirankarn, Nattiya, Chamnanphon, Monpat, Suttichet, Thitima B., Shotelersuk, Vorasuk, Pongpanich, Monnat, Phokaew, Chureerat, Chetruengchai, Wanna, Putchareon, Opa, Torvorapanit, Pattama, Puthanakit, Thanyawee, Suchartlikitwong, Pintip, Nilaratanakul, Voraphoj, Sodsai, Pimpayao, Brumpton, Ben M., Hveem, Kristian, Willer, Cristen, Zhou, Wei, Rogne, Tormod, Solligard, Erik, Åsvold, Bjørn Olav, Franke, Lude, Boezen, Marike, Deelen, Patrick, Claringbould, Annique, Lopera, Esteban, Warmerdam, Robert, Vonk, Judith. M., van Blokland, Irene, Lanting, Pauline, Ori, Anil P. S., Feng, Yen-Chen Anne, Mercader, Josep, Weiss, Scott T., Karlson, Elizabeth W., Smoller, Jordan W., Murphy, Shawn N., Meigs, James B., Woolley, Ann E., Green, Robert C., Perez, Emma F., Zöllner, Sebastian, Wang, Jiongming, Beck, Andrew, Sloofman, Laura G., Ascolillo, Steven, Sebra, Robert P., Collins, Brett L., Levy, Te, Buxbaum, Joseph D., Sealfon, Stuart C., Jordan, Daniel M., Thompson, Ryan C., Gettler, Kyle, Chaudhary, Kumardeep, Belbin, Gillian M., Preuss, Michael, Hoggart, Clive, Choi, Sam, Underwood, Slayton J., Salib, Irene, Britvan, Bari, Keller, Katherine, Tang, Lara, Peruggia, Michael, Hiester, Liam L., Niblo, Kristi, Aksentijevich, Alexandra, Labkowsky, Alexander, Karp, Avromie, Zlatopolsky, Menachem, Zyndorf, Marissa, Charney, Alexander W., Beckmann, Noam D., Schadt, Eric E., Abul-Husn, Noura S., Cho, Judy H., Itan, Yuval, Kenny, Eimear E., Loos, Ruth J. F., Nadkarni, Girish N., Do, Ron, O’Reilly, Paul, Huckins, Laura M., Ferreira, Manuel A. R., Abecasis, Goncalo R., Leader, Joseph B., Cantor, Michael N., Justice, Anne E., Carey, Dave J., Chittoor, Geetha, Josyula, Navya Shilpa, Kosmicki, Jack A., Horowitz, Julie E., Baras, Ari, Gass, Matthew C., Yadav, Ashish, Mirshahi, Tooraj, Hottenga, Jouke Jan, Bartels, Meike, de geus, Eco E. J. C., Nivard, Michel M. G., Verma, Anurag, Ritchie, Marylyn D., Rader, Daniel, Li, Binglan, Verma, Shefali S., Lucas, Anastasia, Bradford, Yuki, Abedalthagafi, Malak, Alaamery, Manal, Alshareef, Abdulraheem, Sawaji, Mona, Massadeh, Salam, AlMalik, Abdulaziz, Alqahtani, Saleh, Baraka, Dona, Harthi, Fawz Al, Alsolm, Ebtehal, Safieh, Leen Abu, Alowayn, Albandary M., Alqubaishi, Fatimah, Mutairi, Amal Al, Mangul, Serghei, Almutairi, Mansour, Aljawini, Nora, Albesher, Nour, Arabi, Yaseen M., Mahmoud, Ebrahim S., Khattab, Amin K., Halawani, Roaa T., Alahmadey, Ziab Z., Albakri, Jehad K., Felemban, Walaa A., Suliman, Bandar A., Hasanato, Rana, Al-Awdah, Laila, Alghamdi, Jahad, AlZahrani, Deema, AlJohani, Sameera, Al-Afghani, Hani, AlDhawi, Nouf, AlBardis, Hadeel, Alkwai, Sarah, Alswailm, Moneera, Almalki, Faisal, Albeladi, Maha, Almohammed, Iman, Barhoush, Eman, Albader, Anoud, Alotaibi, Sara, Alghamdi, Bader, Jung, Junghyun, fawzy, Mohammad S., Alrashed, May, Zeberg, Hugo, Nkambul, Lindo, Frithiof, Robert, Hultström, Michael, Lipcsey, Miklo, Tardif, Nicola, Rooyackers, Olav, Grip, Jonathan, Maricic, Tomislav, Helgeland, Øyvind, Magnus, Per, Trogstad, Lill-Iren S., Lee, Yunsung, Harris, Jennifer R., Mangino, Massimo, Spector, Tim D., Emma, Duncan, Moutsianas, Louka, Caulfield, Mark J., Scott, Richard H., Kousathanas, Athanasio, Pasko, Dorota, Walker, Susan, Stuckey, Alex, Odhams, Christopher A., Rhodes, Daniel, Fowler, Tom, Rendon, Augusto, Chan, Georgia, Arumugam, Prabhu, Karczewski, Konrad J., Martin, Alicia R., Wilson, Daniel J., Spencer, Chris C. A., Crook, Derrick W., Wyllie, David H., O’Connell, Anne Marie, Atkinson, Elizabeth G., Tsuo, Kristin, Baya, Nikola, Turley, Patrick, Gupta, Rahul, Walters, Raymond K., Palmer, Duncan S., Sarma, Gopal, Cheng, Nathan, Lu, Wenhan, Churchhouse, Claire, Goldstein, Jacqueline I., King, Daniel, Seed, Cotton, Daly, Mark J., Finucane, Hilary, Bryant, Sam, Satterstrom, F. Kyle, Band, Gavin, Earle, Sarah G., Lin, Shang-Kuan, Arning, Nicola, Koelling, Nil, Armstrong, Jacob, Rudkin, Justine K., Callier, Shawneequa, Cusick, Caroline, Soranzo, Nicole, Zhao, Jing Hua, Danesh, John, Angelantonio, Emanuele Di, Butterworth, Adam S., Sun, Yan V., Huffman, Jennifer E., Cho, Kelly, O’Donnell, Christopher J., Tsao, Phil, Gaziano, J. Michael, Peloso, Gina, Ho, Yuk-Lam, Smieszek, Sandra P., Polymeropoulos, Christo, Polymeropoulos, Vasilio, Polymeropoulos, Mihael H., Przychodzen, Bartlomiej P., Fernandez-Cadenas, Israel, Planas, Anna M., Perez-Tur, Jordi, Llucià-Carol, Laia, Cullell, Natalia, Muiño, Elena, Cárcel-Márquez, Jara, DeDiego, Marta L., Iglesias, Lara Lloret, Soriano, Alex, Rico, Veronica, Agüero, Daiana, Bedini, Josep L., Lozano, Francisco, Domingo, Carlo, Robles, Veronica, Ruiz-Jaén, Francisca, Márquez, Leonardo, Gomez, Juan, Coto, Eliecer, Albaiceta, Guillermo M., García-Clemente, Marta, Dalmau, David, Arranz, Maria J., Dietl, Beatriz, Serra-Llovich, Alex, Soler, Pere, Colobrán, Roger, Martín-Nalda, Andrea, Martínez, Alba Parra, Bernardo, David, Rojo, Silvia, Fiz-López, Aida, Arribas, Elisa, de la Cal-Sabater, Paloma, Segura, Tomá, González-Villa, Esther, Serrano-Heras, Gemma, Martí-Fàbregas, Joan, Jiménez-Xarrié, Elena, de Felipe Mimbrera, Alicia, Masjuan, Jaime, García-Madrona, Sebastian, Domínguez-Mayoral, Anna, Villalonga, Joan Montaner, Menéndez-Valladares, Paloma, Chasman, Daniel I., Sesso, Howard D., Manson, JoAnn E., Buring, Julie E., Ridker, Paul M., Franco, Giulianini, Davis, Lea, Lee, Sulggi, Priest, Jame, Sankaran, Vijay G., van Heel, David, Biesecker, Le, Kerchberger, V. Eric, Baillie, J. Kenneth, APH - Personalized Medicine, APH - Health Behaviors & Chronic Diseases, Biological Psychology, APH - Mental Health, AMS - Sports, AMS - Ageing & Vitality, APH - Methodology, Mccurdy, Shannon, Mccormick, Joseph B., Macarthur, Daniel, Maclaughlin, Bev, Lefaive, Jonathon, Almalik, Abdulaziz, Alzahrani, Deema, Aljohani, Sameera, Aldhawi, Nouf, Albardis, Hadeel, Fawzy, Mohammad S., Dediego, Marta L., Stem Cell Aging Leukemia and Lymphoma (SALL), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Life Course Epidemiology (LCE), Groningen Research Institute for Asthma and COPD (GRIAC), University of Zurich, COVID-19 Host Genetics Initiative, Barcelona Supercomputing Center, COVID-19 Genetics Initiative, including authors, Institute for Molecular Medicine Finland, and Data Science Genetic Epidemiology Lab
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Informàtica::Aplicacions de la informàtica::Bioinformàtica [Àrees temàtiques de la UPC] ,Quantitative Trait Loci ,MUC5B PROMOTER POLYMORPHISM ,Genome-wide association studies ,COVID-19 (Malaltia) ,UFSP13-7 Evolution in Action: From Genomes to Ecosystems ,COVID-19 (Disease) ,Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA ,SDG 3 - Good Health and Well-being ,Humans ,genetics ,Genetic variation ,Genomes ,Medicinsk genetik ,1000 Multidisciplinary ,Multidisciplinary ,Chromosome Mapping ,COVID-19 ,Human Genetics ,10124 Institute of Molecular Life Sciences ,covid-19 ,3121 General medicine, internal medicine and other clinical medicine ,570 Life sciences ,biology ,Medical Genetics - Abstract
Matters arising from: Mapping the human genetic architecture of COVID-19 Original Article published on 08 July 2021 https://www.nature.com/articles/s41586-021-03767-x The COVID-19 pandemic continues to pose a major public health threat, especially in countries with low vaccination rates. To better understand the biological underpinnings of SARS-CoV-2 infection and COVID-19 severity, we formed the COVID-19 Host Genetics Initiative1. Here we present a genome-wide association study meta-analysis of up to 125,584 cases and over 2.5 million control individuals across 60 studies from 25 countries, adding 11 genome-wide significant loci compared with those previously identified2. Genes at new loci, including SFTPD, MUC5B and ACE2, reveal compelling insights regarding disease susceptibility and severity. Here we present meta-analyses bringing together 60 studies from 25 countries (Fig. 1 and Supplementary Table 1) for three COVID-19-related phenotypes: (1) individuals critically ill with COVID-19 on the basis of requiring respiratory support in hospital or who died as a consequence of the disease (9,376 cases, of which 3,197 are new in this data release, and 1,776,645 control individuals); (2) individuals with moderate or severe COVID-19 defined as those hospitalized due to symptoms associated with the infection (25,027 cases, 11,386 new and 2,836,272 control individuals); and (3) all cases with reported SARS-CoV-2 infection regardless of symptoms (125,584 cases, 76,022 new and 2,575,347 control individuals). Most studies have reported results before the roll out of the COVID-19 vaccination campaign. An overview of the study design is provided in Supplementary Fig. 1. We found a total of 23 genome-wide significant loci (P
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- 2022
4. Biology, Population Fluctuation, and Foliar Consumption Rate of Durrantia arcanella Busk, 1912 (Lepidoptera: Depressariidae), a Defoliator of Oil Palm in the Colombian Caribbean.
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Tejeda-Rico, German E., Barrios-Trilleras, Carlos E., Diaz-Castro, Roberto J., Florián-Martínez, Leidy V., Contreras-Arias, Leidy J., Padilla-Agudelo, José Luis, and Morales-Rodríguez, Anuar
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LIFE cycles (Biology) , *BIOLOGY , *LEPIDOPTERA , *PHYTOPHAGOUS insects , *POPULATION dynamics , *LARVAE , *OIL palm , *PUPAE , *PALMS - Abstract
Simple Summary: Colombia currently has 595,722 oil palm-cultivated hectares, but production is declining due to phytophagous insects feeding mainly on the leaves; one of them, Durrantia arcanella, is a recurring pest in the northern palm zone of Colombia, for which we do not have all the essential information. Therefore, it was proposed to determine its biology, foliar consumption rate, population fluctuation, and relationship with climatic variables and to identify its main natural enemies in the department of Cesar. The life cycle under laboratory conditions, including adult longevity, was 48.0 ± 10.1 days, the egg stage lasted 8.0 ± 0.7 days, the larva stage lasted 24.2 ± 6.2 days, the pre-pupa stage lasted 1.5 ± 0.5 days, the pupa stage lasted 7.1 ± 0.9 and the adult had a longevity of 7.2 ± 2.0 days. At the end of the larval period, it was determined that they individually consumed 8.2 ± 5.3 cm2 of leaflets. Correlation was found between D. arcanella population dynamics and climatic factors such as temperature and relative humidity, likewise with natural enemies. Durrantia arcanella is a recurring pest insect of oil palm in Colombia. Because the biology and ecology of D. arcanella are unknown, it was proposed to determine the life cycle and foliar consumption under laboratory conditions. Furthermore, through sequential sampling for two and a half years, its population fluctuation and natural enemies were determined in Agustín Codazzi and El Copey (Cesar, Colombia). Also, temperature, precipitation, and relative humidity were registered. The life cycle of D. arcanella lasted 48.0 ± 10.1 days, the egg 8.0 ± 0.7 days, larva 24.2 ± 6.2 days, pre-pupa 1.5 ± 0.5 days, pupa 7.1 ± 0.9 days, and adult 7.2 ± 2.0 days. The larvae consumed 8.2 ± 5.3 cm2 of leaflets. Correlations were found between the population fluctuation in D. arcanella and the temperature in El Copey (ρ = −0.45; p < 0.0043), relative humidity in Codazzi (ρ = 0.33; p < 0.034), and with the natural control in both locations ((ρ = 0, 61; p < 0.000044) and (ρ = 0.42; p < 0.006)). These results suggest monitoring the pest populations in the second semester of the year and show the importance of promoting native natural enemies. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Differential and shared genetic effects on kidney function between diabetic and non-diabetic individuals
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Winkler, Thomas W., Rasheed, Humaira, Teumer, Alexander, Gorski, Mathias, Rowan, Bryce X., Stanzick, Kira J., Thomas, Laurent F., Tin, Adrienne, Hoppmann, Anselm, Chu, Audrey Y., Tayo, Bamidele, Thio, Chris H. L., Cusi, Daniele, Chai, Jin-Fang, Sieber, Karsten B., Horn, Katrin, Li, Man, Scholz, Markus, Cocca, Massimiliano, Wuttke, Matthias, van der Most, Peter J., Yang, Qiong, Ghasemi, Sahar, Nutile, Teresa, Li, Yong, Pontali, Giulia, Günther, Felix, Dehghan, Abbas, Correa, Adolfo, Parsa, Afshin, Feresin, Agnese, de Vries, Aiko P. J., Zonderman, Alan B., Smith, Albert V., Oldehinkel, Albertine J., De Grandi, Alessandro, Rosenkranz, Alexander R., Franke, Andre, Teren, Andrej, Metspalu, Andres, Hicks, Andrew A., Morris, Andrew P., Tönjes, Anke, Morgan, Anna, Podgornaia, Anna I., Peters, Annette, Körner, Antje, Mahajan, Anubha, Campbell, Archie, Freedman, Barry I., Spedicati, Beatrice, Ponte, Belen, Schöttker, Ben, Brumpton, Ben, Banas, Bernhard, Krämer, Bernhard K., Jung, Bettina, Åsvold, Bjørn Olav, Smith, Blair H., Ning, Boting, Penninx, Brenda W. J. H., Vanderwerff, Brett R., Psaty, Bruce M., Kammerer, Candace M., Langefeld, Carl D., Hayward, Caroline, Spracklen, Cassandra N., Robinson-Cohen, Cassianne, Hartman, Catharina A., Lindgren, Cecilia M., Wang, Chaolong, Sabanayagam, Charumathi, Heng, Chew-Kiat, Lanzani, Chiara, Khor, Chiea-Chuen, Cheng, Ching-Yu, Fuchsberger, Christian, Gieger, Christian, Shaffer, Christian M., Schulz, Christina-Alexandra, Willer, Cristen J., Chasman, Daniel I., Gudbjartsson, Daniel F., Ruggiero, Daniela, Toniolo, Daniela, Czamara, Darina, Porteous, David J., Waterworth, Dawn M., Mascalzoni, Deborah, Mook-Kanamori, Dennis O., Reilly, Dermot F., Daw, E. Warwick, Hofer, Edith, Boerwinkle, Eric, Salvi, Erika, Bottinger, Erwin P., Tai, E-Shyong, Catamo, Eulalia, Rizzi, Federica, Guo, Feng, Rivadeneira, Fernando, Guilianini, Franco, Sveinbjornsson, Gardar, Ehret, Georg, Waeber, Gerard, Biino, Ginevra, Girotto, Giorgia, Pistis, Giorgio, Nadkarni, Girish N., Delgado, Graciela E., Montgomery, Grant W., Snieder, Harold, Campbell, Harry, White, Harvey D., Gao, He, Stringham, Heather M., Schmidt, Helena, Li, Hengtong, Brenner, Hermann, Holm, Hilma, Kirsten, Holger, Kramer, Holly, Rudan, Igor, Nolte, Ilja M., Tzoulaki, Ioanna, Olafsson, Isleifur, Martins, Jade, Cook, James P., Wilson, James F., Halbritter, Jan, Felix, Janine F., Divers, Jasmin, Kooner, Jaspal S., Lee, Jeannette Jen-Mai, O’Connell, Jeffrey, Rotter, Jerome I., Liu, Jianjun, Xu, Jie, Thiery, Joachim, Ärnlöv, Johan, Kuusisto, Johanna, Jakobsdottir, Johanna, Tremblay, Johanne, Chambers, John C., Whitfield, John B., Gaziano, John M., Marten, Jonathan, Coresh, Josef, Jonas, Jost B., Mychaleckyj, Josyf C., Christensen, Kaare, Eckardt, Kai-Uwe, Mohlke, Karen L., Endlich, Karlhans, Dittrich, Katalin, Ryan, Kathleen A., Rice, Kenneth M., Taylor, Kent D., Ho, Kevin, Nikus, Kjell, Matsuda, Koichi, Strauch, Konstantin, Miliku, Kozeta, Hveem, Kristian, Lind, Lars, Wallentin, Lars, Yerges-Armstrong, Laura M., Raffield, Laura M., Phillips, Lawrence S., Launer, Lenore J., Lyytikäinen, Leo-Pekka, Lange, Leslie A., Citterio, Lorena, Klaric, Lucija, Ikram, M. Arfan, Ising, Marcus, Kleber, Marcus E., Francescatto, Margherita, Concas, Maria Pina, Ciullo, Marina, Piratsu, Mario, Orho-Melander, Marju, Laakso, Markku, Loeffler, Markus, Perola, Markus, de Borst, Martin H., Gögele, Martin, Bianca, Martina La, Lukas, Mary Ann, Feitosa, Mary F., Biggs, Mary L., Wojczynski, Mary K., Kavousi, Maryam, Kanai, Masahiro, Akiyama, Masato, Yasuda, Masayuki, Nauck, Matthias, Waldenberger, Melanie, Chee, Miao-Li, Chee, Miao-Ling, Boehnke, Michael, Preuss, Michael H., Stumvoll, Michael, Province, Michael A., Evans, Michele K., O’Donoghue, Michelle L., Kubo, Michiaki, Kähönen, Mika, Kastarinen, Mika, Nalls, Mike A., Kuokkanen, Mikko, Ghanbari, Mohsen, Bochud, Murielle, Josyula, Navya Shilpa, Martin, Nicholas G., Tan, Nicholas Y. Q., Palmer, Nicholette D., Pirastu, Nicola, Schupf, Nicole, Verweij, Niek, Hutri-Kähönen, Nina, Mononen, Nina, Bansal, Nisha, Devuyst, Olivier, Melander, Olle, Raitakari, Olli T., Polasek, Ozren, Manunta, Paolo, Gasparini, Paolo, Mishra, Pashupati P., Sulem, Patrick, Magnusson, Patrik K. E., Elliott, Paul, Ridker, Paul M., Hamet, Pavel, Svensson, Per O., Joshi, Peter K., Kovacs, Peter, Pramstaller, Peter P., Rossing, Peter, Vollenweider, Peter, van der Harst, Pim, Dorajoo, Rajkumar, Sim, Ralene Z. H., Burkhardt, Ralph, Tao, Ran, Noordam, Raymond, Mägi, Reedik, Schmidt, Reinhold, de Mutsert, Renée, Rueedi, Rico, van Dam, Rob M., Carroll, Robert J., Gansevoort, Ron T., Loos, Ruth J. F., Felicita, Sala Cinzia, Sedaghat, Sanaz, Padmanabhan, Sandosh, Freitag-Wolf, Sandra, Pendergrass, Sarah A., Graham, Sarah E., Gordon, Scott D., Hwang, Shih-Jen, Kerr, Shona M., Vaccargiu, Simona, Patil, Snehal B., Hallan, Stein, Bakker, Stephan J. L., Lim, Su-Chi, Lucae, Susanne, Vogelezang, Suzanne, Bergmann, Sven, Corre, Tanguy, Ahluwalia, Tarunveer S., Lehtimäki, Terho, Boutin, Thibaud S., Meitinger, Thomas, Wong, Tien-Yin, Bergler, Tobias, Rabelink, Ton J., Esko, Tõnu, Haller, Toomas, Thorsteinsdottir, Unnur, Völker, Uwe, Foo, Valencia Hui Xian, Salomaa, Veikko, Vitart, Veronique, Giedraitis, Vilmantas, Gudnason, Vilmundur, Jaddoe, Vincent W. V., Huang, Wei, Zhang, Weihua, Wei, Wen Bin, Kiess, Wieland, März, Winfried, Koenig, Wolfgang, Lieb, Wolfgang, Gao, Xin, Sim, Xueling, Wang, Ya Xing, Friedlander, Yechiel, Tham, Yih-Chung, Kamatani, Yoichiro, Okada, Yukinori, Milaneschi, Yuri, Yu, Zhi, Hung, Adriana M., Stark, Klaus J., Stefansson, Kari, Böger, Carsten A., Kronenberg, Florian, Köttgen, Anna, Pattaro, Cristian, Heid, Iris M., Psychiatry, APH - Mental Health, Amsterdam Neuroscience - Complex Trait Genetics, Amsterdam Neuroscience - Mood, Anxiety, Psychosis, Stress & Sleep, APH - Digital Health, University of Helsinki, CAMM - Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, Research Programs Unit, Tampere University, Clinical Medicine, TAYS Heart Centre, Department of Clinical Chemistry, Department of Clinical Physiology and Nuclear Medicine, Internal Medicine, Pediatrics, Epidemiology, Radiology & Nuclear Medicine, Erasmus MC other, Home Office, Imperial College Healthcare NHS Trust- BRC Funding, Medical Research Council (MRC), UK DRI Ltd, Interdisciplinary Centre Psychopathology and Emotion regulation (ICPE), Life Course Epidemiology (LCE), Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC), Cardiovascular Centre (CVC), Winkler, Thomas W, Rasheed, Humaira, Teumer, Alexander, Gorski, Mathia, Rowan, Bryce X, Stanzick, Kira J, Thomas, Laurent F, Tin, Adrienne, Hoppmann, Anselm, Chu, Audrey Y, Tayo, Bamidele, Thio, Chris H L, Cusi, Daniele, Chai, Jin-Fang, Sieber, Karsten B, Horn, Katrin, Li, Man, Scholz, Marku, Cocca, Massimiliano, Wuttke, Matthia, van der Most, Peter J, Yang, Qiong, Ghasemi, Sahar, Nutile, Teresa, Li, Yong, Pontali, Giulia, Günther, Felix, Dehghan, Abba, Correa, Adolfo, Parsa, Afshin, Feresin, Agnese, de Vries, Aiko P J, Zonderman, Alan B, Smith, Albert V, Oldehinkel, Albertine J, De Grandi, Alessandro, Rosenkranz, Alexander R, Franke, Andre, Teren, Andrej, Metspalu, Andre, Hicks, Andrew A, Morris, Andrew P, Tönjes, Anke, Morgan, Anna, Podgornaia, Anna I, Peters, Annette, Körner, Antje, Mahajan, Anubha, Campbell, Archie, Freedman, Barry I, Spedicati, Beatrice, Ponte, Belen, Schöttker, Ben, Brumpton, Ben, Banas, Bernhard, Krämer, Bernhard K, Jung, Bettina, Åsvold, Bjørn Olav, Smith, Blair H, Ning, Boting, Penninx, Brenda W J H, Vanderwerff, Brett R, Psaty, Bruce M, Kammerer, Candace M, Langefeld, Carl D, Hayward, Caroline, Spracklen, Cassandra N, Robinson-Cohen, Cassianne, Hartman, Catharina A, Lindgren, Cecilia M, Wang, Chaolong, Sabanayagam, Charumathi, Heng, Chew-Kiat, Lanzani, Chiara, Khor, Chiea-Chuen, Cheng, Ching-Yu, Fuchsberger, Christian, Gieger, Christian, Shaffer, Christian M, Schulz, Christina-Alexandra, Willer, Cristen J, Chasman, Daniel I, Gudbjartsson, Daniel F, Ruggiero, Daniela, Toniolo, Daniela, Czamara, Darina, Porteous, David J, Waterworth, Dawn M, Mascalzoni, Deborah, Mook-Kanamori, Dennis O, Reilly, Dermot F, Daw, E Warwick, Hofer, Edith, Boerwinkle, Eric, Salvi, Erika, Bottinger, Erwin P, Tai, E-Shyong, Catamo, Eulalia, Rizzi, Federica, Guo, Feng, Rivadeneira, Fernando, Guilianini, Franco, Sveinbjornsson, Gardar, Ehret, Georg, Waeber, Gerard, Biino, Ginevra, Girotto, Giorgia, Pistis, Giorgio, Nadkarni, Girish N, Delgado, Graciela E, Montgomery, Grant W, Snieder, Harold, Campbell, Harry, White, Harvey D, Gao, He, Stringham, Heather M, Schmidt, Helena, Li, Hengtong, Brenner, Hermann, Holm, Hilma, Kirsten, Holgen, Kramer, Holly, Rudan, Igor, Nolte, Ilja M, Tzoulaki, Ioanna, Olafsson, Isleifur, Martins, Jade, Cook, James P, Wilson, James F, Halbritter, Jan, Felix, Janine F, Divers, Jasmin, Kooner, Jaspal S, Lee, Jeannette Jen-Mai, O'Connell, Jeffrey, Rotter, Jerome I, Liu, Jianjun, Xu, Jie, Thiery, Joachim, Ärnlöv, Johan, Kuusisto, Johanna, Jakobsdottir, Johanna, Tremblay, Johanne, Chambers, John C, Whitfield, John B, Gaziano, John M, Marten, Jonathan, Coresh, Josef, Jonas, Jost B, Mychaleckyj, Josyf C, Christensen, Kaare, Eckardt, Kai-Uwe, Mohlke, Karen L, Endlich, Karlhan, Dittrich, Katalin, Ryan, Kathleen A, Rice, Kenneth M, Taylor, Kent D, Ho, Kevin, Nikus, Kjell, Matsuda, Koichi, Strauch, Konstantin, Miliku, Kozeta, Hveem, Kristian, Lind, Lar, Wallentin, Lar, Yerges-Armstrong, Laura M, Raffield, Laura M, Phillips, Lawrence S, Launer, Lenore J, Lyytikäinen, Leo-Pekka, Lange, Leslie A, Citterio, Lorena, Klaric, Lucija, Ikram, M Arfan, Ising, Marcu, Kleber, Marcus E, Francescatto, Margherita, Concas, Maria Pina, Ciullo, Marina, Piratsu, Mario, Orho-Melander, Marju, Laakso, Markku, Loeffler, Marku, Perola, Marku, de Borst, Martin H, Gögele, Martin, Bianca, Martina La, Lukas, Mary Ann, Feitosa, Mary F, Biggs, Mary L, Wojczynski, Mary K, Kavousi, Maryam, Kanai, Masahiro, Akiyama, Masato, Yasuda, Masayuki, Nauck, Matthia, Waldenberger, Melanie, Chee, Miao-Li, Chee, Miao-Ling, Boehnke, Michael, Preuss, Michael H, Stumvoll, Michael, Province, Michael A, Evans, Michele K, O'Donoghue, Michelle L, Kubo, Michiaki, Kähönen, Mika, Kastarinen, Mika, Nalls, Mike A, Kuokkanen, Mikko, Ghanbari, Mohsen, Bochud, Murielle, Josyula, Navya Shilpa, Martin, Nicholas G, Tan, Nicholas Y Q, Palmer, Nicholette D, Pirastu, Nicola, Schupf, Nicole, Verweij, Niek, Hutri-Kähönen, Nina, Mononen, Nina, Bansal, Nisha, Devuyst, Olivier, Melander, Olle, Raitakari, Olli T, Polasek, Ozren, Manunta, Paolo, Gasparini, Paolo, Mishra, Pashupati P, Sulem, Patrick, Magnusson, Patrik K E, Elliott, Paul, Ridker, Paul M, Hamet, Pavel, Svensson, Per O, Joshi, Peter K, Kovacs, Peter, Pramstaller, Peter P, Rossing, Peter, Vollenweider, Peter, van der Harst, Pim, Dorajoo, Rajkumar, Sim, Ralene Z H, Burkhardt, Ralph, Tao, Ran, Noordam, Raymond, Mägi, Reedik, Schmidt, Reinhold, de Mutsert, Renée, Rueedi, Rico, van Dam, Rob M, Carroll, Robert J, Gansevoort, Ron T, Loos, Ruth J F, Felicita, Sala Cinzia, Sedaghat, Sanaz, Padmanabhan, Sandosh, Freitag-Wolf, Sandra, Pendergrass, Sarah A, Graham, Sarah E, Gordon, Scott D, Hwang, Shih-Jen, Kerr, Shona M, Vaccargiu, Simona, Patil, Snehal B, Hallan, Stein, Bakker, Stephan J L, Lim, Su-Chi, Lucae, Susanne, Vogelezang, Suzanne, Bergmann, Sven, Corre, Tanguy, Ahluwalia, Tarunveer S, Lehtimäki, Terho, Boutin, Thibaud S, Meitinger, Thoma, Wong, Tien-Yin, Bergler, Tobia, Rabelink, Ton J, Esko, Tõnu, Haller, Tooma, Thorsteinsdottir, Unnur, Völker, Uwe, Foo, Valencia Hui Xian, Salomaa, Veikko, Vitart, Veronique, Giedraitis, Vilmanta, Gudnason, Vilmundur, Jaddoe, Vincent W V, Huang, Wei, Zhang, Weihua, Wei, Wen Bin, Kiess, Wieland, März, Winfried, Koenig, Wolfgang, Lieb, Wolfgang, Gao, Xin, Sim, Xueling, Wang, Ya Xing, Friedlander, Yechiel, Tham, Yih-Chung, Kamatani, Yoichiro, Okada, Yukinori, Milaneschi, Yuri, Yu, Zhi, Stark, Klaus J, Stefansson, Kari, Böger, Carsten A, Hung, Adriana M, Kronenberg, Florian, Köttgen, Anna, Pattaro, Cristian, Heid, Iris M, and Lee Kong Chian School of Medicine (LKCMedicine)
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Life Sciences & Biomedicine - Other Topics ,EXPRESSION ,Diabetic Nephropathies/genetics ,610 Medizin ,LOCI ,Medicine (miscellaneous) ,EFFICIENT ,Lifelines cohort study ,Kidney ,General Biochemistry, Genetics and Molecular Biology ,DISEASE ,QUALITY-CONTROL ,SDG 3 - Good Health and Well-being ,Diabetic Nephropathy ,Diabetes Mellitus ,Humans ,Medicine [Science] ,Diabetic Nephropathies ,GENOME-WIDE ASSOCIATION ,Biology ,DiscovEHR/MyCode study ,METAANALYSIS ,Glomerular Filtration Rate/genetics ,Medicinsk genetik ,ddc:610 ,Science & Technology ,genetic ,effects ,kidney ,diabetic ,JOINT ,Klinisk medicin ,Diabetes Mellitu ,3126 Surgery, anesthesiology, intensive care, radiology ,Multidisciplinary Sciences ,ENVIRONMENT INTERACTION ,Creatinine ,VA Million Veteran Program ,Science & Technology - Other Topics ,3111 Biomedicine ,Clinical Medicine ,SMOKING ,General Agricultural and Biological Sciences ,Life Sciences & Biomedicine ,Medical Genetics ,Human ,Genome-Wide Association Study ,Glomerular Filtration Rate - Abstract
Reduced glomerular filtration rate (GFR) can progress to kidney failure. Risk factors include genetics and diabetes mellitus (DM), but little is known about their interaction. We conducted genome-wide association meta-analyses for estimated GFR based on serum creatinine (eGFR), separately for individuals with or without DM (nDM = 178,691, nnoDM = 1,296,113). Our genome-wide searches identified (i) seven eGFR loci with significant DM/noDM-difference, (ii) four additional novel loci with suggestive difference and (iii) 28 further novel loci (including CUBN) by allowing for potential difference. GWAS on eGFR among DM individuals identified 2 known and 27 potentially responsible loci for diabetic kidney disease. Gene prioritization highlighted 18 genes that may inform reno-protective drug development. We highlight the existence of DM-only and noDM-only effects, which can inform about the target group, if respective genes are advanced as drug targets. Largely shared effects suggest that most drug interventions to alter eGFR should be effective in DM and noDM. Published version The Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) supported the meta-analysis—Project-ID 387509280—SFB1350 (Subproject C6 to I.M.H.). A.M.H., B.R., and R.T. were supported by VACSR&D MVP grant CX001897. This research is based on data from the Million Veteran Program, Office of Research and Development, Veterans Health Administration, and was supported by VACSR&D MVP grant CX001897 (A.M.H.). This publication does not represent the views of the Department of Veteran Affairs or the United States Government. We conducted this research using the UK Biobank resource under the application number 20272.
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- 2022
6. Chronic aryl hydrocarbon receptor activity phenocopies smoking‐induced skeletal muscle impairment
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José A. Morais, Tanja Taivassalo, Russell T. Hepple, Angela Rico de Souza, Sarah K. Burke, Trace Thome, Vijayendran Chandran, Carolyn J. Baglole, Alexander John Willms, R. Thomas Jagoe, Jean Bourbeau, Maria-Eleni Anagnostou, Yana Goddard, Kayla Miguez, Liam F. Fitzgerald, and Terence E. Ryan
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medicine.medical_specialty ,Sarcopenia ,Cachexia ,Neuromuscular junction ,Diseases of the musculoskeletal system ,Mice ,Pulmonary Disease, Chronic Obstructive ,Atrophy ,Physiology (medical) ,Internal medicine ,Smoke ,medicine ,Animals ,Humans ,Orthopedics and Sports Medicine ,Muscle, Skeletal ,Aryl hydrocarbon receptor activity ,biology ,Myogenesis ,business.industry ,Smoking ,QM1-695 ,Skeletal muscle ,medicine.disease ,Aryl hydrocarbon receptor ,Muscle atrophy ,medicine.anatomical_structure ,Endocrinology ,Receptors, Aryl Hydrocarbon ,RC925-935 ,Human anatomy ,biology.protein ,medicine.symptom ,business - Abstract
Background Chronic obstructive pulmonary disease (COPD) patients exhibit skeletal muscle atrophy, denervation, and reduced mitochondrial oxidative capacity. Whilst chronic tobacco smoke exposure is implicated in COPD muscle impairment, the mechanisms involved are ambiguous. The aryl hydrocarbon receptor (AHR) is a ligand‐activated transcription factor that activates detoxifying pathways with numerous exogenous ligands, including tobacco smoke. Whereas transient AHR activation is adaptive, chronic activation can be toxic. On this basis, we tested the hypothesis that chronic smoke‐induced AHR activation causes adverse muscle impact. Methods We used clinical patient muscle samples, and in vitro (C2C12 myotubes) and in vivo models (mouse), to perform gene expression, mitochondrial function, muscle and neuromuscular junction morphology, and genetic manipulations (adeno‐associated virus‐mediated gene transfer). Results Sixteen weeks of tobacco smoke exposure in mice caused muscle atrophy, neuromuscular junction degeneration, and reduced oxidative capacity. Similarly, smoke exposure reprogrammed the muscle transcriptome, with down‐regulation of mitochondrial and neuromuscular junction genes. In mouse and human patient specimens, smoke exposure increased muscle AHR signalling. Mechanistically, experiments in cultured myotubes demonstrated that smoke condensate activated the AHR, caused mitochondrial impairments, and induced an AHR‐dependent myotube atrophy. Finally, to isolate the role of AHR activity, expression of a constitutively active AHR mutant without smoke exposure caused atrophy and mitochondrial impairments in cultured myotubes, and muscle atrophy and neuromuscular junction degeneration in mice. Conclusions These results establish that chronic AHR activity, as occurs in smokers, phenocopies the atrophy, mitochondrial impairment, and neuromuscular junction degeneration caused by chronic tobacco smoke exposure.
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- 2022
7. One maternal lineage leads the expansion of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) in the New and Old Worlds
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Sandra I. Mayorga, Jerson Vanderlei Carús Guedes, Leonardo Rodrigues Barbosa, André Garcia, Tania Novoselsky, Dayanna do Nascimento Machado, Jonas André Arnemann, Gevork Arakelian, Donald R. Hodel, Horacio Silva, Gustavo A. Ugalde, Juan Manuel Vanegas-Rico, Clérison Régis Perini, Sergio Ramos, Stefania Laudonia, Ervandil Corrêa Costa, Manuela Branco, Ivair Valmorbida, Gonzalo Martínez, Eduardo Jiménez-Quiroz, Dayanna do N. Machado, Doutoranda da UFSM, Ervandil C. Costa, Departamento de Defesa Fitossanitária, Jerson V. C. Guedes, Departamento de Defesa Fitossanitária, LEONARDO RODRIGUES BARBOSA, CNPF, Gonzalo Martínez, Instituto Nacional de Investigación Agropecuaria, Sandra I. Mayorga, Servicio Agrícola y Ganadero, Sergio O. Ramos, Instituto Nacional de Tecnología Agropecuaria, Manuela Branco, Universidade de Lisboa, André Garcia, Universidade de Lisboa, Juan Manuel Vanegas-Rico, Facultad de Estudios Superiores Iztacala, UNAM, Eduardo Jiménez-Quiroz, Laboratorio de Análisis y Referencia en Sanidad Forestal, Stefania Laudonia, Università degli Studi di Napoli Federico II, Tania Novoselsky, Tel Aviv University, Donald R. Hodel, University of California, Cooperative Extension, Gevork Arakelian, Entomologist, Los Angeles County Agricultural Commissioner, Horacio Silva, Faculdad de Agronomía Universidad de la República Uruguay, Clérison R. Perini, Departamento de Defesa Fitossanitária, Ivair Valmorbida, Iowa State University, Gustavo A. Ugalde, Iowa State University, Jonas A. Arnemann, Iowa State University., Machado, Dayanna do N., Costa, Ervandil C., Guedes, Jerson V. C., Barbosa, Leonardo R., Martínez, Gonzalo, Mayorga, Sandra I., Ramos, Sergio O., Branco, Manuela, Garcia, André, Vanegas-Rico, Juan Manuel, Jiménez-Quiroz, Eduardo, Laudonia, Stefania, Novoselsky, Tania, Hodel, Donald R., Arakelian, Gevork, Silva, Horacio, Perini, Clérison R., Valmorbida, Ivair, Ugalde, Gustavo A., and Arnemann, Jonas A.
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0106 biological sciences ,Asia ,Thaumastocoris peregrinus ,Molecular biology ,Lineage (evolution) ,Zoology ,lcsh:Medicine ,História da invasão global ,DNA, Mitochondrial ,010603 evolutionary biology ,01 natural sciences ,Article ,Electron Transport Complex IV ,Heteroptera ,First record ,Peregrinus ,Bronze bug ,Animals ,MtDNA ,Percevejo bronzeado ,lcsh:Science ,Eucalyptus ,Multidisciplinary ,Corymbia ,biology ,Biological techniques ,Myrtaceae ,Haplotype ,lcsh:R ,Australia ,Global invasion history ,Leptocybe invasa ,Genetic Variation ,South America ,Praga invasora ,biology.organism_classification ,Praga de Planta ,Hemiptera ,Europe ,Invasive pests ,010602 entomology ,Geography ,Haplotypes ,North America ,Insect Proteins ,lcsh:Q ,PEST analysis - Abstract
The bronze bug, Thaumastocoris peregrinus, an Australian native insect, has become a nearly worldwide invasive pest in the last 16 years and has been causing signifcant damage to eucalypts (Myrtaceae), including Eucalyptus spp. and Corymbia spp. Its rapid expansion leads to new questions about pathways and routes that T. peregrinus used to invade other continents and countries. We used mtDNA to characterize specimens of T. peregrinus collected from 10 countries where this species has become established, including six recently invaded countries: Chile, Israel, Mexico, Paraguay, Portugal, and the United States of America. We then combined our mtDNA data with previous data available from South Africa, Australia, and Europe to construct a world mtDNA network of haplotypes. Haplotype A was the most common present in all specimens of sites sampled in the New World, Europe, and Israel, however from Australia second more frequently. Haplotype D was the most common one from native populations in Australia. Haplotype A difers from the two major haplotypes found in South Africa (D and G), confrming that at least two independent invasions occurred, one from Australia to South Africa, and the other one from Australia to South America (A). In conclusion, Haplotype A has an invasion success over many countries in the World. Additionally, analyzing data from our work and previous reports, it is possible to suggest some invasive routes of T. peregrinus to predict such events and support preventive control measures. Made available in DSpace on 2020-09-12T04:36:44Z (GMT). No. of bitstreams: 1 Leonardo-2020-Machado-et-al-One-maternal-lineage-leads-the-expansion-of-Thaumastocoris-peregrinus.pdf: 3236777 bytes, checksum: 90255ede5fe4812b249279669eee2238 (MD5) Previous issue date: 2020
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- 2020
8. BTK inhibition sensitizes acute lymphoblastic leukemia to asparaginase by suppressing the amino acid response pathway
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Beat Bornhauser, Roland P. Kuiper, Steven M. Kornblau, Britt M.T. Vervoort, Jiangyan Yu, Rico Hagelaar, Fieke W Hoff, Frank N. van Leeuwen, Dorette van Ingen Schenau, Maria Pamela Dobay, Trisha M Tee, Laurens T. van der Meer, Silvia Jenni, Jules P.P. Meijerink, Jean-Pierre Bourquin, Miriam Butler, University of Zurich, and van Leeuwen, Frank N
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Treatment response ,Asparaginase ,1303 Biochemistry ,Lymphoblastic Leukemia ,Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] ,Immunology ,2720 Hematology ,Antineoplastic Agents ,Apoptosis ,610 Medicine & health ,Biochemistry ,1307 Cell Biology ,Mice ,chemistry.chemical_compound ,Piperidines ,Cell Line, Tumor ,Agammaglobulinaemia Tyrosine Kinase ,Tumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14] ,Animals ,Humans ,CRISPR ,Bruton's tyrosine kinase ,Amino Acids ,chemistry.chemical_classification ,2403 Immunology ,biology ,Adenine ,Cell Biology ,Hematology ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Amino acid ,chemistry ,10036 Medical Clinic ,Ibrutinib ,Cancer research ,biology.protein ,Tyrosine kinase ,Signal Transduction - Abstract
Contains fulltext : 241347.pdf (Publisher’s version ) (Closed access) Asparaginase (ASNase) therapy has been a mainstay of acute lymphoblastic leukemia (ALL) protocols for decades and shows promise in the treatment of a variety of other cancers. To improve the efficacy of ASNase treatment, we used a CRISPR/Cas9-based screen to identify actionable signaling intermediates that improve the response to ASNase. Both genetic inactivation of Bruton's tyrosine kinase (BTK) and pharmacological inhibition by the BTK inhibitor ibrutinib strongly synergize with ASNase by inhibiting the amino acid response pathway, a mechanism involving c-Myc-mediated suppression of GCN2 activity. This synthetic lethal interaction was observed in 90% of patient-derived xenografts, regardless of the genomic subtype. Moreover, ibrutinib substantially improved ASNase treatment response in a murine PDX model. Hence, ibrutinib may be used to enhance the clinical efficacy of ASNase in ALL. This trial was registered at www.clinicaltrials.gov as # NCT02884453.
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- 2021
9. Allosteric Modulation of GSK-3β as a New Therapeutic Approach in Limb Girdle Muscular Dystrophy R1 Calpain 3-Related
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Leire Casas-Fraile, Valle Palomo, Garazi Guembelzu, Anabel Rico, Ana Aiastui, Amets Sáenz, Adolfo López de Munain, Andrea Valls, Ana Martínez, Ministerio de Economía y Competitividad (España), European Commission, Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España), Instituto de Salud Carlos III, Asociación Guipuzcoana de Enfermos Neuromusculares, Eusko Jaurlaritza, Rico, Anabel [0000-0002-3002-6688], Palomo, Valle [0000-0002-1473-4086], Martínez, Ana [0000-0002-2707-8110], Casas-Fraile, Leire [0000-0002-7504-6578], Valls, Andrea [0000-0002-9211-4034], López de Munain, Adolfo [0000-0002-9509-4032], Sáenz, Amets [0000-0002-0704-1150], Rico, Anabel, Palomo, Valle, Martínez, Ana, Casas-Fraile, Leire, Valls, Andrea, López de Munain, Adolfo, and Sáenz, Amets
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0301 basic medicine ,Muscle Fibers, Skeletal ,Muscle Proteins ,Quinolones ,Adenosine Triphosphate ,0302 clinical medicine ,CAPN3 ,GSK-3 ,VP0.7 ,Phosphorylation ,Muscular dystrophy ,Biology (General) ,Wnt Signaling Pathway ,Spectroscopy ,biology ,Calpain ,limb-girdle muscular dystrophy ,TOR Serine-Threonine Kinases ,Intracellular Signaling Peptides and Proteins ,Wnt signaling pathway ,General Medicine ,CD56 Antigen ,LGMDR1 ,Computer Science Applications ,Cell biology ,Chemistry ,Hydrazines ,mTOR ,Limb-girdle muscular dystrophy ,Allosteric Site ,Signal Transduction ,QH301-705.5 ,Nerve Tissue Proteins ,Article ,Catalysis ,Inorganic Chemistry ,tideglusib ,03 medical and health sciences ,Wnt ,Thiadiazoles ,medicine ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,QD1-999 ,PI3K/AKT/mTOR pathway ,Glycogen Synthase Kinase 3 beta ,business.industry ,GSK-3β ,Tideglusib ,Organic Chemistry ,Dystrophy ,Fibroblasts ,medicine.disease ,030104 developmental biology ,Gene Expression Regulation ,Muscular Dystrophies, Limb-Girdle ,biology.protein ,business ,Protein Processing, Post-Translational ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery - Abstract
18 p.-8 fig.-1 tab., Limb-girdle muscular dystrophy R1 calpain 3-related (LGMDR1) is an autosomal recessive muscular dystrophy produced by mutations in the CAPN3 gene. It is a rare disease and there is no cure or treatment for the disease while the pathophysiological mechanism by which the absence of calpain 3 provokes the dystrophy in muscles is not clear. However, key proteins implicated in Wnt and mTOR signaling pathways, which regulate muscle homeostasis, showed a considerable reduction in their expression and in their phosphorylation in LGMDR1 patients’ muscles. Finally, the administration of tideglusib and VP0.7, ATP non-competitive inhibitors of glycogen synthase kinase 3β (GSK-3β), restore the expression and phosphorylation of these proteins in LGMDR1 cells, opening the possibility of their use as therapeutic options, This work was financed through the grants received from the Health Research Fund (PI16/01325, PI17/01841 and DTS19/00061) of the Spanish Ministry of Economy and Competitiveness and the European Union (ERDF) and it was in part supported by the Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED: CB06/05/1126 to A.R., G.G., A.V., L.C.-F., A.L.d.M. and A.S. and CB18/05/00040 to A.M. and V.P.), Carlos III Health Institute and by GENE (Association of Neuromuscular diseases of Gipuzkoa). A.R. was supported by the predoctoral fellowship given by the department of Education, Universities and Research of the Basque Government (PRE-2016-1-0382).
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- 2021
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10. SARS-CoV-2 in Transit: Characterization of SARS-CoV-2 Genomes From Venezuelan Migrants in Colombia
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Jayeeta Dutta, Ana S. Gonzalez-Reiche, Angelica Rico, Maria E. Grillet, Carolina Hernández, Liseth Pardo, Emilia Mia Sordillo, Harm van Bakel, Viviana Simon, Zenab Khan, Matthew M. Hernandez, Juan David Ramírez, Marina Muñoz, Sergio Castañeda, Luz H. Patiño, Adriana van de Guchte, Carolina Flórez, Nathalia Ballesteros, Sergio Gomez, Carlos E. Hernandez-Pereira, Alberto Paniz-Mondolfi, and Lourdes A. Delgado-Noguera
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Microbiology (medical) ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,L18F ,Infectious and parasitic diseases ,RC109-216 ,Biology ,Colombia ,Genome ,Genotype ,Humans ,Lineages ,Phylogeny ,Venezuelan-Colombian border ,Infectivity ,Transients and Migrants ,Phylogenetic tree ,SARS-CoV-2 ,COVID-19 ,virus diseases ,General Medicine ,Infectious Diseases ,Evolutionary biology ,Close relationship ,Mutations ,Reference genome - Abstract
Objectives To evaluate the genomic epidemiology of SARS-CoV-2 from Venezuelan migrants living in Colombia. Methods This study sequenced SARS-CoV-2 from 30 clinical specimens collected from Venezuelan migrants. Genomes were compared with the Wuhan reference genome to identify polymorphisms, reconstruct phylogenetic relationships and perform comparative genomic analyses. Geographic, sociodemographic and clinical data were also studied across genotypes. Results This study demonstrated the presence of six distinct SARS-CoV-2 lineages circulating among Venezuelan migrants, as well as a close relationship between SARS-CoV-2 genomic sequences obtained from individuals living in the Venezuelan-Colombian border regions of La Guajira (Colombia) and Zulia (Venezuela). Three clusters (C-1, C-2 and C-3) were well supported by phylogenomic inference, supporting the hypothesis of three potential transmission routes across the Colombian-Venezuelan border. These genomes included point mutations previously associated with increased infectivity. A mutation (L18F) in the N-terminal domain of the spike protein that has been associated with compromised binding of neutralizing antibodies was found in 2 of 30 (6.6%) genomes. A statistically significant association was identified with symptomatology for cluster C2. Conclusion The close phylogenetic relationships between SARS-CoV-2 genomes from Venezuelan migrants and from people living at the Venezuela-Colombian border support the importance of human movements for the spread of COVID-19 and for emerging virus variants.
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- 2021
11. Quantification of the demands of cricket bowling and the relationship to injury risk: a systematic review
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Matthew Constable, Rodrigo Rico Bini, Michael Kingsley, and Daniel Wundersitz
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education.field_of_study ,medicine.medical_specialty ,biology ,Sports medicine ,business.industry ,Research ,Rehabilitation ,Population ,Physical Therapy, Sports Therapy and Rehabilitation ,Workload ,biology.organism_classification ,Quality of evidence ,Physical medicine and rehabilitation ,Cricket ,Medicine ,Injury risk ,Orthopedics and Sports Medicine ,Duration (project management) ,Role playing ,business ,education ,human activities ,RC1200-1245 ,Uncategorized - Abstract
Background Bowling in cricket is a complex sporting movement which, despite being well characterised, still produces a significant number of injuries each year. Fast bowlers are more likely to be injured than any other playing role. Frequency, duration, intensity and volume of bowling, which have been generalised as measurements of workload, are thought to be risk factors for injuries. Injury rates of fast bowlers have not reduced in recent years despite the implementation of various workload monitoring practices. Objective To identify the variables used to quantify frequency, intensity, time and volume of bowling; and evaluate relationships between these variables and injury risk. Methods Six online databases were systematically searched for studies on fast bowling that included terms related to workload. Population characteristics, variables relating to demand and their relationship to standardised definitions of physical activity were extracted from all included studies. Results Bowling workload is typically quantified through measures of frequency, duration, or indirect intensity, with few studies reporting on bowling volume. Conclusions When reported on, volume was often described using imprecise or insufficient measures of intensity. There is a need to develop more appropriate measures of intensity during bowling and improve the quality of evidence to inform on bowling programme management practices.
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- 2021
12. The aryl hydrocarbon receptor promotes differentiation during mouse preimplantational embryo development
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Ana Nacarino-Palma, Pedro M. Fernández-Salguero, Jaime M. Merino, Francisco J. González-Rico, Claudia M. Rejano-Gordillo, and Ana Ordiales-Talavero
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Homeobox protein NANOG ,Genotype ,Regulator ,Embryonic Development ,Biochemistry ,Article ,Mice ,Hippo ,embryo differentiation ,Genetics ,medicine ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,Hippo Signaling Pathway ,Blastocyst ,Transcription factor ,reproductive and urinary physiology ,biology ,aryl hydrocarbon receptor ,Embryogenesis ,preimplantation ,Gene Expression Regulation, Developmental ,Embryo ,Cell Differentiation ,Cell Biology ,respiratory system ,Aryl hydrocarbon receptor ,pluripotency ,Embryo, Mammalian ,Phenotype ,Cell biology ,respiratory tract diseases ,Oxidative Stress ,Protein Transport ,medicine.anatomical_structure ,Receptors, Aryl Hydrocarbon ,Gene Knockdown Techniques ,embryonic structures ,biology.protein ,biological phenomena, cell phenomena, and immunity ,Energy Metabolism ,Glycolysis ,Octamer Transcription Factor-3 ,Developmental Biology - Abstract
Summary Mammalian embryogenesis is a complex process controlled by transcription factors that regulate the balance between pluripotency and differentiation. Transcription factor aryl hydrocarbon receptor (AhR) regulates OCT4/POU5F1 and NANOG, both essential controllers of pluripotency, stemness and early embryo development. Molecular mechanisms controlling OCT4/POU5F1 and NANOG during embryogenesis remain unidentified. We show that AhR regulates pluripotency factors and maintains the metabolic activity required for proper embryo differentiation. AhR-lacking embryos (AhR−/−) showed a pluripotent phenotype characterized by a delayed expression of trophectoderm differentiation markers. Accordingly, central pluripotency factors OCT4/POU5F1 and NANOG were overexpressed in AhR−/− embryos at initial developmental stages. An altered intracellular localization of these factors was observed in the absence of AhR and, importantly, Oct4 had an opposite expression pattern with respect to AhR from the two-cell stage to blastocyst, suggesting a negative regulation of OCT4/POU5F by AhR. We propose that AhR is a regulator of pluripotency and differentiation in early mouse embryogenesis., Highlights • AhR regulates pluripotency factors OCT4 and NANOG during early embryo differentiation • AhR lacking embryos (AhR−/−) show a pluripotent phenotype • Pluripotent phenotype of AhR−/− embryos show enhanced glycolytic metabolism, In this article, Fernández-Salguero and colleagues show that transcription factor AhR regulates pluripotency factors and maintains the metabolic activity required for proper embryo differentiation. AhR-lacking embryos (AhR−/−) showed a pluripotent phenotype characterized by a delayed expression of trophectoderm differentiation markers. Accordingly, central pluripotency factors were overexpressed in AhR−/− embryos at initial developmental stages.
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- 2021
13. Antimicrobial Activity, in silico Molecular Docking, ADMET and DFT Analysis of Secondary Metabolites from Roots of Three Ethiopian Medicinal Plants
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María Trelis, Diego Cortes, Mathewos Anza, Rajalakshmanan Eswaramoorthy, Jesús Zueco, Hortensia Rico, Milkyas Endale, Luz Cardona, and Belén Abarca
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Euphorbia schimperiana ,Stereochemistry ,Ranunculaceae ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,DNA gyrase ,chemistry.chemical_compound ,DFT analysis ,Uvaria scheffleri ,Lupeol ,Original Research ,Clematis burgensis ,biology ,Dihydrochalcone ,molecular docking ,Coumarin ,Antimicrobial ,biology.organism_classification ,Computer Science Applications ,ADMET ,chemistry ,Advances and Applications in Bioinformatics and Chemistry ,Chemistry (miscellaneous) ,Docking (molecular) ,antimicrobial ,Antibacterial activity - Abstract
Mathewos Anza,1 Milkyas Endale,1 Luz Cardona,2 Diego Cortes,3 Rajalakshmanan Eswaramoorthy,1 Jesus Zueco,4 Hortensia Rico,4 Maria Trelis,5 Belen Abarca2 1Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, Adama, Ethiopia; 2Department of Organic Chemistry, Faculty of Chemistry, University of Valencia, Burjassot, Spain; 3Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Burjassot, Spain; 4Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Burjassot, Spain; 5Parasites and Health Research Group, Department of Pharmacy, Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, University of Valencia, Burjassot, SpainCorrespondence: Mathewos Anza; Milkyas EndaleDepartment of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, P.O. Box 1888, Adama, EthiopiaEmail mathewosanza@gmail.com; milkyas.endale@astu.edu.etBackground: Uvaria scheffleri (Annonaceae), Clematis burgensis (Ranunculaceae), and Euphorbia schimperiana (Euphorbiaceae) are medicinal plants traditionally used to treat cough, tuberculosis, asthma, sore throat and skin infections.Methods: Silica gel column chromatographic separation was used to isolate compounds. Crude extract and isolated compounds were evaluated for antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans via the broth dilution method. Docking studies were performed with E. coli DNA-Gyrase B and human DNA topoisomerase IIα by using AutoDock Vina. ADMET were predicted by SwissADME, PreADMET, and OSIRIS Property predictions. The optimized structures and molecular electrostatic potential surface of the isolated compounds were predicted by DFT analysis using B3LYP/6-31G basis levels.Results: Silica gel column chromatographic separation afforded five compounds 1â 5 of which N-methyl-2,3-bis(2-hydroxybenzyl)-1Н-indol ( 1) is reported herein for the first time, along with known C-benzylated dihydrochalcone uvaretin ( 2), bis(2-ethylheptyl) phthalate ( 3), lupeol ( 4) and suberosin derivative ( 5). Dichloromethane roots extract of U. scheffleri showed potent antibacterial activity against S. aureus (MIC = 6.25 μg/mL) compared to gentamicin (MIC=5 μg/mL). In silico, molecular docking analysis of compounds ( 1and 3â 5) showed strong interaction with E. coli DNA gyrase B with a binding energy value ranging from â 6.9 to â 6.0 kcal/mol compared to ciprofloxacin â 7.2 kcal/mol, whereas analysis against human topoisomerase IIα showed binding energy value ranging from â 5.9 to â 5.3 kcal/mol compared to vosaroxin (â 6.2 kcal/mol).Conclusion: The results obtained suggest that N-methyl-2,3-bis(2-hydroxybenzyl)-1Н-indol ( 1) and coumarin ( 5) are potential topoisomerase II α inhibitors and might be used as anticancer agents. The ADMET studies showed the highest drug-likeness properties for studied compounds other than bis(2-ethylheptyl) phthalate ( 3). DFT calculations suggested that studied compounds showed the lowest gap energy and were chemically reactive, and isolated compounds may serve as potential drug candidates that corroborate with the traditional uses of studied plants.Keywords: Uvaria scheffleri, Clematis burgensis, Euphorbia schimperiana, antimicrobial, molecular docking, ADMET, DFT analysis
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- 2021
14. Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines
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Enos Tangke Arung, Nataniel Tandirogang, Rico Ramadhan, Irawan Wijaya Kusuma, Sukemi, Binti Khairunnisa, Kuniyoshi Shimizu, Yong Ung Kim, Maki Nagata, Naomichi Takemoto, Syafrizal, Masako Matsumoto, Swandari Paramita, Yhiya Amen, and Yadi
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biology ,Traditional medicine ,Stingless bee ,QH301-705.5 ,Cytotoxicity ,H. fimbriata ,Honey bee ,Honey ,Propolis ,biology.organism_classification ,medicine.disease_cause ,HeLa ,Bee pollen ,Pollen ,medicine ,Stingless bees ,Original Article ,Cancer cell lines ,Biology (General) ,General Agricultural and Biological Sciences - Abstract
Our effort to find new material for anti cancer from natural resources leads us to focus on stingless bee products such as honey, bee pollen, and propolis. The products were from seven stingless bees named Homotrigona fimbriata, Heterotrigona itama, Heterotrigona bakeri, Tetragonula sarawakensis, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula laeviceps. The stingless bee products were evaluated for their cytotoxicity effect on MCF-7, HeLa and Caco-2 cancer cell lines. This is the first time to be reported that the honey, ethanol extracts of bee pollen and propolis of H. fimbriata displayed more potent cytotoxicity than other stingless bee products. By chromatography and biological activity-guided fractionation, ethanol extract of propolis from H. fimbriata was fractionated and isolated its active compound named mangiferonic acid. Mangiferonic acid showed a cytotoxicity effect with IC50 values 96.76 µM in MCF-7, >110.04 µM in HeLa, and > 110.04 µM in Caco-2, respectively. These results exhibited the potential of ethanol extracts from propolis of H. fimbriata to be further developed for drug and experiments to verify the function are essential.
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- 2021
15. The Design and Sensitivity of JUNO's scintillator radiopurity pre-detector OSIRIS
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JUNO Collaboration, Abusleme, Angel, Adam, Thomas, Ahmad, Shakeel, Ahmed, Rizwan, Aiello, Sebastiano, Akram, Muhammad, An, Fengpeng, An, Guangpeng, An, Qi, Andronico, Giuseppe, Anfimov, Nikolay, Antonelli, Vito, Antoshkina, Tatiana, Asavapibhop, Burin, de André, João Pedro Athayde Marcondes, Auguste, Didier, Babic, Andrej, Baldini, Wander, Barresi, Andrea, Basilico, Davide, Baussan, Eric, Bellato, Marco, Bergnoli, Antonio, Birkenfeld, Thilo, Blin, Sylvie, Blum, David, Blyth, Simon, Bolshakova, Anastasia, Bongrand, Mathieu, Bordereau, Clément, Breton, Dominique, Brigatti, Augusto, Brugnera, Riccardo, Bruno, Riccardo, Budano, Antonio, Buscemi, Mario, Busto, Jose, Butorov, Ilya, Cabrera, Anatael, Cai, Hao, Cai, Xiao, Cai, Yanke, Cai, Zhiyan, Cammi, Antonio, Campeny, Agustin, Cao, Chuanya, Cao, Guofu, Cao, Jun, Caruso, Rossella, Cerna, Cédric, Chang, Jinfan, Chang, Yun, Chen, Pingping, Chen, Po-An, Chen, Shaomin, Chen, Xurong, Chen, Yi-Wen, Chen, Yixue, Chen, Yu, Chen, Zhang, Cheng, Jie, Cheng, Yaping, Chetverikov, Alexey, Chiesa, Davide, Chimenti, Pietro, Chukanov, Artem, Claverie, Gérard, Clementi, Catia, Clerbaux, Barbara, Di Lorenzo, Selma Conforti, Corti, Daniele, Costa, Salvatore, Corso, Flavio Dal, Dalager, Olivia, De La Taille, Christophe, Deng, Jiawei, Deng, Zhi, Deng, Ziyan, Depnering, Wilfried, Diaz, Marco, Ding, Xuefeng, Ding, Yayun, Dirgantara, Bayu, Dmitrievsky, Sergey, Dohnal, Tadeas, Dolzhikov, Dmitry, Donchenko, Georgy, Dong, Jianmeng, Doroshkevich, Evgeny, Dracos, Marcos, Druillole, Frédéric, Du, Shuxian, Dusini, Stefano, Dvorak, Martin, Enqvist, Timo, Enzmann, Heike, Fabbri, Andrea, Fajt, Lukas, Fan, Donghua, Fan, Lei, Fang, Can, Fang, Jian, Fang, Wenxing, Fargetta, Marco, Fedoseev, Dmitry, Fekete, Vladko, Feng, Li-Cheng, Feng, Qichun, Ford, Richard, Formozov, Andrey, Fournier, Amélie, Gan, Haonan, Gao, Feng, Garfagnini, Alberto, Genster, Christoph, Giammarchi, Marco, Giaz, Agnese, Giudice, Nunzio, Gonchar, Maxim, Gong, Guanghua, Gong, Hui, Gornushkin, Yuri, Göttel, Alexandre, Grassi, Marco, Grewing, Christian, Gromov, Vasily, Gu, Minghao, Gu, Xiaofei, Gu, Yu, Guan, Mengyun, Guardone, Nunzio, Gul, Maria, Guo, Cong, Guo, Jingyuan, Guo, Wanlei, Guo, Xinheng, Guo, Yuhang, Hackspacher, Paul, Hagner, Caren, Han, Ran, Han, Yang, Hassan, Muhammad Sohaib, He, Miao, He, Wei, Heinz, Tobias, Hellmuth, Patrick, Heng, Yuekun, Herrera, Rafael, Hong, Daojin, Hor, YuenKeung, Hou, Shaojing, Hsiung, Yee, Hu, Bei-Zhen, Hu, Hang, Hu, Jianrun, Hu, Jun, Hu, Shouyang, Hu, Tao, Hu, Zhuojun, Huang, Chunhao, Huang, Guihong, Huang, Hanxiong, Huang, Wenhao, Huang, Xin, Huang, Xingtao, Huang, Yongbo, Hui, Jiaqi, Huo, Lei, Huo, Wenju, Huss, Cédric, Hussain, Safeer, Ioannisian, Ara, Isocrate, Roberto, Jelmini, Beatrice, Jen, Kuo-Lun, Jeria, Ignacio, Ji, Xiaolu, Ji, Xingzhao, Jia, Huihui, Jia, Junji, Jian, Siyu, Jiang, Di, Jiang, Xiaoshan, Jin, Ruyi, Jing, Xiaoping, Jollet, Cécile, Joutsenvaara, Jari, Jungthawan, Sirichok, Kalousis, Leonidas, Kampmann, Philipp, Kang, Li, Karagounis, Michael, Kazarian, Narine, Khan, Waseem, Khosonthongkee, Khanchai, Korablev, Denis, Kouzakov, Konstantin, Krasnoperov, Alexey, Kruth, Andre, Kutovskiy, Nikolay, Kuusiniemi, Pasi, Lachenmaier, Tobias, Landini, Cecilia, Leblanc, Sébastien, Lebrin, Victor, Lefevre, Frederic, Lei, Ruiting, Leitner, Rupert, Leung, Jason, Li, Demin, Li, Fei, Li, Fule, Li, Haitao, Li, Huiling, Li, Jiaqi, Li, Mengzhao, Li, Min, Li, Nan, Li, Qingjiang, Li, Ruhui, Li, Shanfeng, Li, Tao, Li, Weidong, Li, Weiguo, Li, Xiaomei, Li, Xiaonan, Li, Xinglong, Li, Yi, Li, Yufeng, Li, Zhaohan, Li, Zhibing, Li, Ziyuan, Liang, Hao, Liang, Jingjing, Liao, Jiajun, Liebau, Daniel, Limphirat, Ayut, Limpijumnong, Sukit, Lin, Guey-Lin, Lin, Shengxin, Lin, Tao, Ling, Jiajie, Lippi, Ivano, Liu, Fang, Liu, Haidong, Liu, Hongbang, Liu, Hongjuan, Liu, Hongtao, Liu, Hui, Liu, Jianglai, Liu, Jinchang, Liu, Min, Liu, Qian, Liu, Qin, Liu, Runxuan, Liu, Shuangyu, Liu, Shubin, Liu, Shulin, Liu, Xiaowei, Liu, Xiwen, Liu, Yan, Liu, Yunzhe, Lokhov, Alexey, Lombardi, Paolo, Lombardo, Claudio, Loo, Kai, Lu, Chuan, Lu, Haoqi, Lu, Jingbin, Lu, Junguang, Lu, Shuxiang, Lu, Xiaoxu, Lubsandorzhiev, Bayarto, Lubsandorzhiev, Sultim, Ludhova, Livia, Luo, Fengjiao, Luo, Guang, Luo, Pengwei, Luo, Shu, Luo, Wuming, Lyashuk, Vladimir, Ma, Bangzheng, Ma, Qiumei, Ma, Si, Ma, Xiaoyan, Ma, Xubo, Maalmi, Jihane, Malyshkin, Yury, Mantovani, Fabio, Manzali, Francesco, Mao, Xin, Mao, Yajun, Mari, Stefano M., Marini, Filippo, Marium, Sadia, Martellini, Cristina, Martin-Chassard, Gisele, Martini, Agnese, Mayilyan, Davit, Mednieks, Ints, Meng, Yue, Meregaglia, Anselmo, Meroni, Emanuela, Meyhöfer, David, Mezzetto, Mauro, Miller, Jonathan, Miramonti, Lino, Montini, Paolo, Montuschi, Michele, Müller, Axel, Muralidharan, Pavithra, Nastasi, Massimiliano, Naumov, Dmitry V., Naumova, Elena, Navas-Nicolas, Diana, Nemchenok, Igor, NguyenThi, MinhThuan, Ning, Feipeng, Ning, Zhe, Nunokawa, Hiroshi, Oberauer, Lothar, Ochoa-Ricoux, Juan Pedro, Olshevskiy, Alexander, Orestano, Domizia, Ortica, Fausto, Othegraven, Rainer, Pan, Hsiao-Ru, Paoloni, Alessandro, Parkalian, Nina, Parmeggiano, Sergio, Pei, Yatian, Pelliccia, Nicomede, Peng, Anguo, Peng, Haiping, Perrot, Frédéric, Petitjean, Pierre-Alexandre, Petrucci, Fabrizio, Pilarczyk, Oliver, Rico, Luis Felipe Piñeres, Popov, Artyom, Poussot, Pascal, Pratumwan, Wathan, Previtali, Ezio, Qi, Fazhi, Qi, Ming, Qian, Sen, Qian, Xiaohui, Qian, Zhen, Qiao, Hao, Qin, Zhonghua, Qiu, Shoukang, Rajput, Muhammad Usman, Ranucci, Gioacchino, Raper, Neill, Re, Alessandra, Rebber, Henning, Rebii, Abdel, Ren, Bin, Ren, Jie, Ricci, Barbara, Robens, Markus, Roche, Mathieu, Rodphai, Narongkiat, Romani, Aldo, Roskovec, Bedřich, Roth, Christian, Ruan, Xiangdong, Ruan, Xichao, Rujirawat, Saroj, Rybnikov, Arseniy, Sadovsky, Andrey, Saggese, Paolo, Sanfilippo, Simone, Sangka, Anut, Sanguansak, Nuanwan, Sawangwit, Utane, Sawatzki, Julia, Sawy, Fatma, Schever, Michaela, Schwab, Cédric, Schweizer, Konstantin, Selyunin, Alexandr, Serafini, Andrea, Settanta, Giulio, Settimo, Mariangela, Shao, Zhuang, Sharov, Vladislav, Shaydurova, Arina, Shi, Jingyan, Shi, Yanan, Shutov, Vitaly, Sidorenkov, Andrey, Šimkovic, Fedor, Sirignano, Chiara, Siripak, Jaruchit, Sisti, Monica, Slupecki, Maciej, Smirnov, Mikhail, Smirnov, Oleg, Sogo-Bezerra, Thiago, Sokolov, Sergey, Songwadhana, Julanan, Soonthornthum, Boonrucksar, Sotnikov, Albert, Šrámek, Ondřej, Sreethawong, Warintorn, Stahl, Achim, Stanco, Luca, Stankevich, Konstantin, Štefánik, Dušan, Steiger, Hans, Steinmann, Jochen, Sterr, Tobias, Stock, Matthias Raphael, Strati, Virginia, Studenikin, Alexander, Sun, Gongxing, Sun, Shifeng, Sun, Xilei, Sun, Yongjie, Sun, Yongzhao, Suwonjandee, Narumon, Szelezniak, Michal, Tang, Jian, Tang, Qiang, Tang, Quan, Tang, Xiao, Tietzsch, Alexander, Tkachev, Igor, Tmej, Tomas, Treskov, Konstantin, Triossi, Andrea, Troni, Giancarlo, Trzaska, Wladyslaw, Tuve, Cristina, Ushakov, Nikita, Boom, Johannes van den, van Waasen, Stefan, Vanroyen, Guillaume, Vassilopoulos, Nikolaos, Vedin, Vadim, Verde, Giuseppe, Vialkov, Maxim, Viaud, Benoit, Vollbrecht, Cornelius, Volpe, Cristina, Vorobel, Vit, Voronin, Dmitriy, Votano, Lucia, Walker, Pablo, Wang, Caishen, Wang, Chung-Hsiang, Wang, En, Wang, Guoli, Wang, Jian, Wang, Jun, Wang, Kunyu, Wang, Lu, Wang, Meifen, Wang, Meng, Wang, Ruiguang, Wang, Siguang, Wang, Wei, Wang, Wenshuai, Wang, Xi, Wang, Xiangyue, Wang, Yangfu, Wang, Yaoguang, Wang, Yi, Wang, Yifang, Wang, Yuanqing, Wang, Yuman, Wang, Zhe, Wang, Zheng, Wang, Zhimin, Wang, Zongyi, Waqas, Muhammad, Watcharangkool, Apimook, Wei, Lianghong, Wei, Wei, Wei, Wenlu, Wei, Yadong, Wen, Liangjian, Wiebusch, Christopher, Wong, Steven Chan-Fai, Wonsak, Bjoern, Wu, Diru, Wu, Fangliang, Wu, Qun, Wu, Zhi, Wurm, Michael, Wurtz, Jacques, Wysotzki, Christian, Xi, Yufei, Xia, Dongmei, Xie, Yuguang, Xie, Zhangquan, Xing, Zhizhong, Xu, Benda, Xu, Cheng, Xu, Donglian, Xu, Fanrong, Xu, Hangkun, Xu, Jilei, Xu, Jing, Xu, Meihang, Xu, Yin, Xu, Yu, Yan, Baojun, Yan, Taylor, Yan, Wenqi, Yan, Xiongbo, Yan, Yupeng, Yang, Anbo, Yang, Changgen, Yang, Huan, Yang, Jie, Yang, Lei, Yang, Xiaoyu, Yang, Yifan, Yao, Haifeng, Yasin, Zafar, Ye, Jiaxuan, Ye, Mei, Ye, Ziping, Yegin, Ugur, Yermia, Frédéric, Yi, Peihuai, Yin, Na, Yin, Xiangwei, You, Zhengyun, Yu, Boxiang, Yu, Chiye, Yu, Chunxu, Yu, Hongzhao, Yu, Miao, Yu, Xianghui, Yu, Zeyuan, Yu, Zezhong, Yuan, Chengzhuo, Yuan, Ying, Yuan, Zhenxiong, Yuan, Ziyi, Yue, Baobiao, Zafar, Noman, Zambanini, Andre, Zavadskyi, Vitalii, Zeng, Shan, Zeng, Tingxuan, Zeng, Yuda, Zhan, Liang, Zhang, Aiqiang, Zhang, Feiyang, Zhang, Guoqing, Zhang, Haiqiong, Zhang, Honghao, Zhang, Jiawen, Zhang, Jie, Zhang, Jingbo, Zhang, Jinnan, Zhang, Peng, Zhang, Qingmin, Zhang, Shiqi, Zhang, Shu, Zhang, Tao, Zhang, Xiaomei, Zhang, Xuantong, Zhang, Xueyao, Zhang, Yan, Zhang, Yinhong, Zhang, Yiyu, Zhang, Yongpeng, Zhang, Yuanyuan, Zhang, Yumei, Zhang, Zhenyu, Zhang, Zhijian, Zhao, Fengyi, Zhao, Jie, Zhao, Rong, Zhao, Shujun, Zhao, Tianchi, Zheng, Dongqin, Zheng, Hua, Zheng, Minshan, Zheng, Yangheng, Zhong, Weirong, Zhou, Jing, Zhou, Li, Zhou, Nan, Zhou, Shun, Zhou, Tong, Zhou, Xiang, Zhu, Jiang, Zhu, Kangpu, Zhu, Kejun, Zhu, Zhihang, Zhuang, Bo, Zhuang, Honglin, Zong, Liang, Zou, Jiaheng, Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des 2 Infinis Irène Joliot-Curie (IJCLab), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique subatomique et des technologies associées (SUBATECH), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), JUNO, Abusleme, A, Adam, T, Ahmad, S, Ahmed, R, Aiello, S, Akram, M, An, F, An, G, An, Q, Andronico, G, Anfimov, N, Antonelli, V, Antoshkina, T, Asavapibhop, B, de Andre, J, Auguste, D, Babic, A, Baldini, W, Barresi, A, Basilico, D, Baussan, E, Bellato, M, Bergnoli, A, Birkenfeld, T, Blin, S, Blum, D, Blyth, S, Bolshakova, A, Bongrand, M, Bordereau, C, Breton, D, Brigatti, A, Brugnera, R, Bruno, R, Budano, A, Buscemi, M, Busto, J, Butorov, I, Cabrera, A, Cai, H, Cai, X, Cai, Y, Cai, Z, Cammi, A, Campeny, A, Cao, C, Cao, G, Cao, J, Caruso, R, Cerna, C, Chang, J, Chang, Y, Chen, P, Chen, S, Chen, X, Chen, Y, Chen, Z, Cheng, J, Cheng, Y, Chetverikov, A, Chiesa, D, Chimenti, P, Chukanov, A, Claverie, G, Clementi, C, Clerbaux, B, Lorenzo, S, Corti, D, Costa, S, Corso, F, Dalager, O, De La Taille, C, Deng, J, Deng, Z, Depnering, W, Diaz, M, Ding, X, Ding, Y, Dirgantara, B, Dmitrievsky, S, Dohnal, T, Dolzhikov, D, Donchenko, G, Dong, J, Doroshkevich, E, Dracos, M, Druillole, F, Du, S, Dusini, S, Dvorak, M, Enqvist, T, Enzmann, H, Fabbri, A, Fajt, L, Fan, D, Fan, L, Fang, C, Fang, J, Fang, W, Fargetta, M, Fedoseev, D, Fekete, V, Feng, L, Feng, Q, Ford, R, Formozov, A, Fournier, A, Gan, H, Gao, F, Garfagnini, A, Genster, C, Giammarchi, M, Giaz, A, Giudice, N, Gonchar, M, Gong, G, Gong, H, Gornushkin, Y, Gottel, A, Grassi, M, Grewing, C, Gromov, V, Gu, M, Gu, X, Gu, Y, Guan, M, Guardone, N, Gul, M, Guo, C, Guo, J, Guo, W, Guo, X, Guo, Y, Hackspacher, P, Hagner, C, Han, R, Han, Y, Hassan, M, He, M, He, W, Heinz, T, Hellmuth, P, Heng, Y, Herrera, R, Hong, D, Hor, Y, Hou, S, Hsiung, Y, Hu, B, Hu, H, Hu, J, Hu, S, Hu, T, Hu, Z, Huang, C, Huang, G, Huang, H, Huang, W, Huang, X, Huang, Y, Hui, J, Huo, L, Huo, W, Huss, C, Hussain, S, Ioannisian, A, Isocrate, R, Jelmini, B, Jen, K, Jeria, I, Ji, X, Jia, H, Jia, J, Jian, S, Jiang, D, Jiang, X, Jin, R, Jing, X, Jollet, C, Joutsenvaara, J, Jungthawan, S, Kalousis, L, Kampmann, P, Kang, L, Karagounis, M, Kazarian, N, Khan, W, Khosonthongkee, K, Korablev, D, Kouzakov, K, Krasnoperov, A, Kruth, A, Kutovskiy, N, Kuusiniemi, P, Lachenmaier, T, Landini, C, Leblanc, S, Lebrin, V, Lefevre, F, Lei, R, Leitner, R, Leung, J, Li, D, Li, F, Li, H, Li, J, Li, M, Li, N, Li, Q, Li, R, Li, S, Li, T, Li, W, Li, X, Li, Y, Li, Z, Liang, H, Liang, J, Liao, J, Liebau, D, Limphirat, A, Limpijumnong, S, Lin, G, Lin, S, Lin, T, Ling, J, Lippi, I, Liu, F, Liu, H, Liu, J, Liu, M, Liu, Q, Liu, R, Liu, S, Liu, X, Liu, Y, Lokhov, A, Lombardi, P, Lombardo, C, Loo, K, Lu, C, Lu, H, Lu, J, Lu, S, Lu, X, Lubsandorzhiev, B, Lubsandorzhiev, S, Ludhova, L, Luo, F, Luo, G, Luo, P, Luo, S, Luo, W, Lyashuk, V, Ma, B, Ma, Q, Ma, S, Ma, X, Maalmi, J, Malyshkin, Y, Mantovani, F, Manzali, F, Mao, X, Mao, Y, Mari, S, Marini, F, Marium, S, Martellini, C, Martin-Chassard, G, Martini, A, Mayilyan, D, Mednieks, I, Meng, Y, Meregaglia, A, Meroni, E, Meyhofer, D, Mezzetto, M, Miller, J, Miramonti, L, Montini, P, Montuschi, M, Muller, A, Muralidharan, P, Nastasi, M, Naumov, D, Naumova, E, Navas-Nicolas, D, Nemchenok, I, Nguyenthi, M, Ning, F, Ning, Z, Nunokawa, H, Oberauer, L, Ochoa-Ricoux, J, Olshevskiy, A, Orestano, D, Ortica, F, Othegraven, R, Pan, H, Paoloni, A, Parkalian, N, Parmeggiano, S, Pei, Y, Pelliccia, N, Peng, A, Peng, H, Perrot, F, Petitjean, P, Petrucci, F, Pilarczyk, O, Rico, L, Popov, A, Poussot, P, Pratumwan, W, Previtali, E, Qi, F, Qi, M, Qian, S, Qian, X, Qian, Z, Qiao, H, Qin, Z, Qiu, S, Rajput, M, Ranucci, G, Raper, N, Re, A, Rebber, H, Rebii, A, Ren, B, Ren, J, Ricci, B, Robens, M, Roche, M, Rodphai, N, Romani, A, Roskovec, B, Roth, C, Ruan, X, Rujirawat, S, Rybnikov, A, Sadovsky, A, Saggese, P, Sanfilippo, S, Sangka, A, Sanguansak, N, Sawangwit, U, Sawatzki, J, Sawy, F, Schever, M, Schwab, C, Schweizer, K, Selyunin, A, Serafini, A, Settanta, G, Settimo, M, Shao, Z, Sharov, V, Shaydurova, A, Shi, J, Shi, Y, Shutov, V, Sidorenkov, A, Simkovic, F, Sirignano, C, Siripak, J, Sisti, M, Slupecki, M, Smirnov, M, Smirnov, O, Sogo-Bezerra, T, Sokolov, S, Songwadhana, J, Soonthornthum, B, Sotnikov, A, Sramek, O, Sreethawong, W, Stahl, A, Stanco, L, Stankevich, K, Stefanik, D, Steiger, H, Steinmann, J, Sterr, T, Stock, M, Strati, V, Studenikin, A, Sun, G, Sun, S, Sun, X, Sun, Y, Suwonjandee, N, Szelezniak, M, Tang, J, Tang, Q, Tang, X, Tietzsch, A, Tkachev, I, Tmej, T, Treskov, K, Triossi, A, Troni, G, Trzaska, W, Tuve, C, Ushakov, N, van den Boom, J, van Waasen, S, Vanroyen, G, Vassilopoulos, N, Vedin, V, Verde, G, Vialkov, M, Viaud, B, Vollbrecht, C, Volpe, C, Vorobel, V, Voronin, D, Votano, L, Walker, P, Wang, C, Wang, E, Wang, G, Wang, J, Wang, K, Wang, L, Wang, M, Wang, R, Wang, S, Wang, W, Wang, X, Wang, Y, Wang, Z, Waqas, M, Watcharangkool, A, Wei, L, Wei, W, Wei, Y, Wen, L, Wiebusch, C, Wong, S, Wonsak, B, Wu, D, Wu, F, Wu, Q, Wu, Z, Wurm, M, Wurtz, J, Wysotzki, C, Xi, Y, Xia, D, Xie, Y, Xie, Z, Xing, Z, Xu, B, Xu, C, Xu, D, Xu, F, Xu, H, Xu, J, Xu, M, Xu, Y, Yan, B, Yan, T, Yan, W, Yan, X, Yan, Y, Yang, A, Yang, C, Yang, H, Yang, J, Yang, L, Yang, X, Yang, Y, Yao, H, Yasin, Z, Ye, J, Ye, M, Ye, Z, Yegin, U, Yermia, F, Yi, P, Yin, N, Yin, X, You, Z, Yu, B, Yu, C, Yu, H, Yu, M, Yu, X, Yu, Z, Yuan, C, Yuan, Y, Yuan, Z, Yue, B, Zafar, N, Zambanini, A, Zavadskyi, V, Zeng, S, Zeng, T, Zeng, Y, Zhan, L, Zhang, A, Zhang, F, Zhang, G, Zhang, H, Zhang, J, Zhang, P, Zhang, Q, Zhang, S, Zhang, T, Zhang, X, Zhang, Y, Zhang, Z, Zhao, F, Zhao, J, Zhao, R, Zhao, S, Zhao, T, Zheng, D, Zheng, H, Zheng, M, Zheng, Y, Zhong, W, Zhou, J, Zhou, L, Zhou, N, Zhou, S, Zhou, T, Zhou, X, Zhu, J, Zhu, K, Zhu, Z, Zhuang, B, Zhuang, H, Zong, L, Zou, J, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Abusleme, Angel, Adam, Thoma, Ahmad, Shakeel, Ahmed, Rizwan, Aiello, Sebastiano, Akram, Muhammad, An, Fengpeng, An, Guangpeng, An, Qi, Andronico, Giuseppe, Anfimov, Nikolay, Antonelli, Vito, Antoshkina, Tatiana, Asavapibhop, Burin, de André, João Pedro Athayde Marconde, Auguste, Didier, Babic, Andrej, Baldini, Wander, Barresi, Andrea, Basilico, Davide, Baussan, Eric, Bellato, Marco, Bergnoli, Antonio, Birkenfeld, Thilo, Blin, Sylvie, Blum, David, Blyth, Simon, Bolshakova, Anastasia, Bongrand, Mathieu, Bordereau, Clément, Breton, Dominique, Brigatti, Augusto, Brugnera, Riccardo, Bruno, Riccardo, Budano, Antonio, Buscemi, Mario, Busto, Jose, Butorov, Ilya, Cabrera, Anatael, Cai, Hao, Cai, Xiao, Cai, Yanke, Cai, Zhiyan, Cammi, Antonio, Campeny, Agustin, Cao, Chuanya, Cao, Guofu, Cao, Jun, Caruso, Rossella, Cerna, Cédric, Chang, Jinfan, Chang, Yun, Chen, Pingping, Chen, Po-An, Chen, Shaomin, Chen, Xurong, Chen, Yi-Wen, Chen, Yixue, Chen, Yu, Chen, Zhang, Cheng, Jie, Cheng, Yaping, Chetverikov, Alexey, Chiesa, Davide, Chimenti, Pietro, Chukanov, Artem, Claverie, Gérard, Clementi, Catia, Clerbaux, Barbara, Lorenzo, Selma Conforti Di, Corti, Daniele, Costa, Salvatore, Corso, Flavio Dal, Dalager, Olivia, De La Taille, Christophe, Deng, Jiawei, Deng, Zhi, Deng, Ziyan, Depnering, Wilfried, Diaz, Marco, Ding, Xuefeng, Ding, Yayun, Dirgantara, Bayu, Dmitrievsky, Sergey, Dohnal, Tadea, Dolzhikov, Dmitry, Donchenko, Georgy, Dong, Jianmeng, Doroshkevich, Evgeny, Dracos, Marco, Druillole, Frédéric, Du, Shuxian, Dusini, Stefano, Dvorak, Martin, Enqvist, Timo, Enzmann, Heike, Fabbri, Andrea, Fajt, Luka, Fan, Donghua, Fan, Lei, Fang, Can, Fang, Jian, Fang, Wenxing, Fargetta, Marco, Fedoseev, Dmitry, Fekete, Vladko, Feng, Li-Cheng, Feng, Qichun, Ford, Richard, Formozov, Andrey, Fournier, Amélie, Gan, Haonan, Gao, Feng, Garfagnini, Alberto, Genster, Christoph, Giammarchi, Marco, Giaz, Agnese, Giudice, Nunzio, Gonchar, Maxim, Gong, Guanghua, Gong, Hui, Gornushkin, Yuri, Göttel, Alexandre, Grassi, Marco, Grewing, Christian, Gromov, Vasily, Gu, Minghao, Gu, Xiaofei, Gu, Yu, Guan, Mengyun, Guardone, Nunzio, Gul, Maria, Guo, Cong, Guo, Jingyuan, Guo, Wanlei, Guo, Xinheng, Guo, Yuhang, Hackspacher, Paul, Hagner, Caren, Han, Ran, Han, Yang, Hassan, Muhammad Sohaib, He, Miao, He, Wei, Heinz, Tobia, Hellmuth, Patrick, Heng, Yuekun, Herrera, Rafael, Hong, Daojin, Hor, Yuenkeung, Hou, Shaojing, Hsiung, Yee, Hu, Bei-Zhen, Hu, Hang, Hu, Jianrun, Hu, Jun, Hu, Shouyang, Hu, Tao, Hu, Zhuojun, Huang, Chunhao, Huang, Guihong, Huang, Hanxiong, Huang, Wenhao, Huang, Xin, Huang, Xingtao, Huang, Yongbo, Hui, Jiaqi, Huo, Lei, Huo, Wenju, Huss, Cédric, Hussain, Safeer, Ioannisian, Ara, Isocrate, Roberto, Jelmini, Beatrice, Jen, Kuo-Lun, Jeria, Ignacio, Ji, Xiaolu, Ji, Xingzhao, Jia, Huihui, Jia, Junji, Jian, Siyu, Jiang, Di, Jiang, Xiaoshan, Jin, Ruyi, Jing, Xiaoping, Jollet, Cécile, Joutsenvaara, Jari, Jungthawan, Sirichok, Kalousis, Leonida, Kampmann, Philipp, Kang, Li, Karagounis, Michael, Kazarian, Narine, Khan, Waseem, Khosonthongkee, Khanchai, Korablev, Deni, Kouzakov, Konstantin, Krasnoperov, Alexey, Kruth, Andre, Kutovskiy, Nikolay, Kuusiniemi, Pasi, Lachenmaier, Tobia, Landini, Cecilia, Leblanc, Sébastien, Lebrin, Victor, Lefevre, Frederic, Lei, Ruiting, Leitner, Rupert, Leung, Jason, Li, Demin, Li, Fei, Li, Fule, Li, Haitao, Li, Huiling, Li, Jiaqi, Li, Mengzhao, Li, Min, Li, Nan, Li, Qingjiang, Li, Ruhui, Li, Shanfeng, Li, Tao, Li, Weidong, Li, Weiguo, Li, Xiaomei, Li, Xiaonan, Li, Xinglong, Li, Yi, Li, Yufeng, Li, Zhaohan, Li, Zhibing, Li, Ziyuan, Liang, Hao, Liang, Jingjing, Liao, Jiajun, Liebau, Daniel, Limphirat, Ayut, Limpijumnong, Sukit, Lin, Guey-Lin, Lin, Shengxin, Lin, Tao, Ling, Jiajie, Lippi, Ivano, Liu, Fang, Liu, Haidong, Liu, Hongbang, Liu, Hongjuan, Liu, Hongtao, Liu, Hui, Liu, Jianglai, Liu, Jinchang, Liu, Min, Liu, Qian, Liu, Qin, Liu, Runxuan, Liu, Shuangyu, Liu, Shubin, Liu, Shulin, Liu, Xiaowei, Liu, Xiwen, Liu, Yan, Liu, Yunzhe, Lokhov, Alexey, Lombardi, Paolo, Lombardo, Claudio, Loo, Kai, Lu, Chuan, Lu, Haoqi, Lu, Jingbin, Lu, Junguang, Lu, Shuxiang, Lu, Xiaoxu, Lubsandorzhiev, Bayarto, Lubsandorzhiev, Sultim, Ludhova, Livia, Luo, Fengjiao, Luo, Guang, Luo, Pengwei, Luo, Shu, Luo, Wuming, Lyashuk, Vladimir, Ma, Bangzheng, Ma, Qiumei, Ma, Si, Ma, Xiaoyan, Ma, Xubo, Maalmi, Jihane, Malyshkin, Yury, Mantovani, Fabio, Manzali, Francesco, Mao, Xin, Mao, Yajun, Mari, Stefano M., Marini, Filippo, Marium, Sadia, Martellini, Cristina, Martin-Chassard, Gisele, Martini, Agnese, Mayilyan, Davit, Mednieks, Int, Meng, Yue, Meregaglia, Anselmo, Meroni, Emanuela, Meyhöfer, 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Sun, Shifeng, Sun, Xilei, Sun, Yongjie, Sun, Yongzhao, Suwonjandee, Narumon, Szelezniak, Michal, Tang, Jian, Tang, Qiang, Tang, Quan, Tang, Xiao, Tietzsch, Alexander, Tkachev, Igor, Tmej, Toma, Treskov, Konstantin, Triossi, Andrea, Troni, Giancarlo, Trzaska, Wladyslaw, Tuve, Cristina, Ushakov, Nikita, van den Boom, Johanne, van Waasen, Stefan, Vanroyen, Guillaume, Vassilopoulos, Nikolao, Vedin, Vadim, Verde, Giuseppe, Vialkov, Maxim, Viaud, Benoit, Vollbrecht, Corneliu, Volpe, Cristina, Vorobel, Vit, Voronin, Dmitriy, Votano, Lucia, Walker, Pablo, Wang, Caishen, Wang, Chung-Hsiang, Wang, En, Wang, Guoli, Wang, Jian, Wang, Jun, Wang, Kunyu, Wang, Lu, Wang, Meifen, Wang, Meng, Wang, Ruiguang, Wang, Siguang, Wang, Wei, Wang, Wenshuai, Wang, Xi, Wang, Xiangyue, Wang, Yangfu, Wang, Yaoguang, Wang, Yi, Wang, Yifang, Wang, Yuanqing, Wang, Yuman, Wang, Zhe, Wang, Zheng, Wang, Zhimin, Wang, Zongyi, Waqas, Muhammad, Watcharangkool, Apimook, Wei, Lianghong, Wei, Wei, Wei, Wenlu, Wei, Yadong, Wen, Liangjian, Wiebusch, Christopher, Wong, Steven Chan-Fai, Wonsak, Bjoern, Wu, Diru, Wu, Fangliang, Wu, Qun, Wu, Zhi, Wurm, Michael, Wurtz, Jacque, Wysotzki, Christian, Xi, Yufei, Xia, Dongmei, Xie, Yuguang, Xie, Zhangquan, Xing, Zhizhong, Xu, Benda, Xu, Cheng, Xu, Donglian, Xu, Fanrong, Xu, Hangkun, Xu, Jilei, Xu, Jing, Xu, Meihang, Xu, Yin, Xu, Yu, Yan, Baojun, Yan, Taylor, Yan, Wenqi, Yan, Xiongbo, Yan, Yupeng, Yang, Anbo, Yang, Changgen, Yang, Huan, Yang, Jie, Yang, Lei, Yang, Xiaoyu, Yang, Yifan, Yao, Haifeng, Yasin, Zafar, Ye, Jiaxuan, Ye, Mei, Ye, Ziping, Yegin, Ugur, Yermia, Frédéric, Yi, Peihuai, Yin, Na, Yin, Xiangwei, You, Zhengyun, Yu, Boxiang, Yu, Chiye, Yu, Chunxu, Yu, Hongzhao, Yu, Miao, Yu, Xianghui, Yu, Zeyuan, Yu, Zezhong, Yuan, Chengzhuo, Yuan, Ying, Yuan, Zhenxiong, Yuan, Ziyi, Yue, Baobiao, Zafar, Noman, Zambanini, Andre, Zavadskyi, Vitalii, Zeng, Shan, Zeng, Tingxuan, Zeng, Yuda, Zhan, Liang, Zhang, Aiqiang, Zhang, Feiyang, Zhang, Guoqing, Zhang, Haiqiong, Zhang, Honghao, Zhang, Jiawen, Zhang, Jie, Zhang, Jingbo, Zhang, Jinnan, Zhang, Peng, Zhang, Qingmin, Zhang, Shiqi, Zhang, Shu, Zhang, Tao, Zhang, Xiaomei, Zhang, Xuantong, Zhang, Xueyao, Zhang, Yan, Zhang, Yinhong, Zhang, Yiyu, Zhang, Yongpeng, Zhang, Yuanyuan, Zhang, Yumei, Zhang, Zhenyu, Zhang, Zhijian, Zhao, Fengyi, Zhao, Jie, Zhao, Rong, Zhao, Shujun, Zhao, Tianchi, Zheng, Dongqin, Zheng, Hua, Zheng, Minshan, Zheng, Yangheng, Zhong, Weirong, Zhou, Jing, Zhou, Li, Zhou, Nan, Zhou, Shun, Zhou, Tong, Zhou, Xiang, Zhu, Jiang, Zhu, Kangpu, Zhu, Kejun, Zhu, Zhihang, Zhuang, Bo, Zhuang, Honglin, Zong, Liang, Zou, Jiaheng, Abusleme A., Adam T., Ahmad S., Ahmed R., Aiello S., Akram M., An F., An G., An Q., Andronico G., Anfimov N., Antonelli V., Antoshkina T., Asavapibhop B., de Andre J.P.A.M., Auguste D., Babic A., Baldini W., Barresi A., Basilico D., Baussan E., Bellato M., Bergnoli A., Birkenfeld T., Blin S., Blum D., Blyth S., Bolshakova A., Bongrand M., Bordereau C., Breton D., Brigatti A., 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C., Gromov V., Gu M., Gu X., Gu Y., Guan M., Guardone N., Gul M., Guo C., Guo J., Guo W., Guo X., Guo Y., Hackspacher P., Hagner C., Han R., Han Y., Hassan M.S., He M., He W., Heinz T., Hellmuth P., Heng Y., Herrera R., Hong D., Hor Y.K., Hou S., Hsiung Y., Hu B.-Z., Hu H., Hu J., Hu S., Hu T., Hu Z., Huang C., Huang G., Huang H., Huang W., Huang X., Huang Y., Hui J., Huo L., Huo W., Huss C., Hussain S., Ioannisian A., Isocrate R., Jelmini B., Jen K.-L., Jeria I., Ji X., Jia H., Jia J., Jian S., Jiang D., Jiang X., Jin R., Jing X., Jollet C., Joutsenvaara J., Jungthawan S., Kalousis L., Kampmann P., Kang L., Karagounis M., Kazarian N., Khan W., Khosonthongkee K., Korablev D., Kouzakov K., Krasnoperov A., Kruth A., Kutovskiy N., Kuusiniemi P., Lachenmaier T., Landini C., Leblanc S., Lebrin V., Lefevre F., Lei R., Leitner R., Leung J., Li D., Li F., Li H., Li J., Li M., Li N., Li Q., Li R., Li S., Li T., Li W., Li X., Li Y., Li Z., Liang H., Liang J., Liao J., Liebau D., Limphirat A., Limpijumnong S., Lin G.-L., Lin S., Lin T., Ling J., Lippi I., Liu F., Liu H., Liu J., Liu M., Liu Q., Liu R., Liu S., Liu X., Liu Y., Lokhov A., Lombardi P., Lombardo C., Loo K., Lu C., Lu H., Lu J., Lu S., Lu X., Lubsandorzhiev B., Lubsandorzhiev S., Ludhova L., Luo F., Luo G., Luo P., Luo S., Luo W., Lyashuk V., Ma B., Ma Q., Ma S., Ma X., Maalmi J., Malyshkin Y., Mantovani F., Manzali F., Mao X., Mao Y., Mari S.M., Marini F., Marium S., Martellini C., Martin-Chassard G., Martini A., Mayilyan D., Mednieks I., Meng Y., Meregaglia A., Meroni E., Meyhofer D., Mezzetto M., Miller J., Miramonti L., Montini P., Montuschi M., Muller A., Muralidharan P., Nastasi M., Naumov D.V., Naumova E., Navas-Nicolas D., Nemchenok I., NguyenThi M.T., Ning F., Ning Z., Nunokawa H., Oberauer L., Ochoa-Ricoux J.P., Olshevskiy A., Orestano D., Ortica F., Othegraven R., Pan H.-R., Paoloni A., Parkalian N., Parmeggiano S., Pei Y., Pelliccia N., Peng A., Peng H., Perrot F., Petitjean P.-A., Petrucci F., Pilarczyk O., Rico L.F.P., Popov A., Poussot P., Pratumwan W., Previtali E., Qi F., Qi M., Qian S., Qian X., Qian Z., Qiao H., Qin Z., Qiu S., Rajput M.U., Ranucci G., Raper N., Re A., Rebber H., Rebii A., Ren B., Ren J., Ricci B., Robens M., Roche M., Rodphai N., Romani A., Roskovec B., Roth C., Ruan X., Rujirawat S., Rybnikov A., Sadovsky A., Saggese P., Sanfilippo S., Sangka A., Sanguansak N., Sawangwit U., Sawatzki J., Sawy F., Schever M., Schwab C., Schweizer K., Selyunin A., Serafini A., Settanta G., Settimo M., Shao Z., Sharov V., Shaydurova A., Shi J., Shi Y., Shutov V., Sidorenkov A., Simkovic F., Sirignano C., Siripak J., Sisti M., Slupecki M., Smirnov M., Smirnov O., Sogo-Bezerra T., Sokolov S., Songwadhana J., Soonthornthum B., Sotnikov A., Sramek O., Sreethawong W., Stahl A., Stanco L., Stankevich K., Stefanik D., Steiger H., Steinmann J., Sterr T., Stock M.R., Strati V., Studenikin A., Sun G., Sun S., Sun X., Sun Y., Suwonjandee N., Szelezniak M., Tang J., Tang Q., Tang X., Tietzsch A., Tkachev I., Tmej T., Treskov K., Triossi A., Troni G., Trzaska W., Tuve C., Ushakov N., van den Boom J., van Waasen S., Vanroyen G., Vassilopoulos N., Vedin V., Verde G., Vialkov M., Viaud B., Vollbrecht C., Volpe C., Vorobel V., Voronin D., Votano L., Walker P., Wang C., Wang C.-H., Wang E., Wang G., Wang J., Wang K., Wang L., Wang M., Wang R., Wang S., Wang W., Wang X., Wang Y., Wang Z., Waqas M., Watcharangkool A., Wei L., Wei W., Wei Y., Wen L., Wiebusch C., Wong S.C.-F., Wonsak B., Wu D., Wu F., Wu Q., Wu Z., Wurm M., Wurtz J., Wysotzki C., Xi Y., Xia D., Xie Y., Xie Z., Xing Z., Xu B., Xu C., Xu D., Xu F., Xu H., Xu J., Xu M., Xu Y., Yan B., Yan T., Yan W., Yan X., Yan Y., Yang A., Yang C., Yang H., Yang J., Yang L., Yang X., Yang Y., Yao H., Yasin Z., Ye J., Ye M., Ye Z., Yegin U., Yermia F., Yi P., Yin N., Yin X., You Z., Yu B., Yu C., Yu H., Yu M., Yu X., Yu Z., Yuan C., Yuan Y., Yuan Z., Yue B., Zafar N., Zambanini A., Zavadskyi V., Zeng S., Zeng T., Zeng Y., Zhan L., Zhang A., Zhang F., Zhang G., Zhang H., Zhang J., Zhang P., Zhang Q., Zhang S., Zhang T., Zhang X., Zhang Y., Zhang Z., Zhao F., Zhao J., Zhao R., Zhao S., Zhao T., Zheng D., Zheng H., Zheng M., Zheng Y., Zhong W., Zhou J., Zhou L., Zhou N., Zhou S., Zhou T., Zhou X., Zhu J., Zhu K., Zhu Z., Zhuang B., Zhuang H., Zong L., and Zou J.
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Liquid scintillator ,Physics - Instrumentation and Detectors ,Physics and Astronomy (miscellaneous) ,Physics::Instrumentation and Detectors ,scintillation counter: liquid ,measurement methods ,QC770-798 ,Astrophysics ,01 natural sciences ,thorium: nuclide ,design [detector] ,neutrino ,Radioactive purity ,Physics ,Low energy neutrino ,JUNO ,liquid [scintillation counter] ,biology ,Settore FIS/01 - Fisica Sperimentale ,Detector ,Instrumentation and Detectors (physics.ins-det) ,3. Good health ,QB460-466 ,Physics::Space Physics ,nuclide [uranium] ,FOS: Physical sciences ,Scintillator ,nuclide [thorium] ,NO ,Nuclear physics ,PE2_2 ,uranium: nuclide ,Nuclear and particle physics. Atomic energy. Radioactivity ,0103 physical sciences ,ddc:530 ,Sensitivity (control systems) ,[PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det] ,010306 general physics ,JUNO, neutrino physics, liquid scintillator ,Engineering (miscellaneous) ,background: radioactivity ,detector: design ,Measurement method ,010308 nuclear & particles physics ,radioactivity [background] ,biology.organism_classification ,sensitivity ,High Energy Physics::Experiment ,Reactor neutrino ,Osiris - Abstract
The European physical journal / C 81(11), 973 (2021). doi:10.1140/epjc/s10052-021-09544-4, The OSIRIS detector is a subsystem of the liquid scintillator filling chain of the JUNO reactor neutrino experiment. Its purpose is to validate the radiopurity of the scintillator to assure that all components of the JUNO scintillator system work to specifications and only neutrino-grade scintillator is filled into the JUNO Central Detector. The aspired sensitivity level of $10^{���16}$ g/g of $^{238}$U and $^{232}$Th requires a large (���20 m$^3$) detection volume and ultralow background levels. The present paper reports on the design and major components of the OSIRIS detector, the detector simulation as well as the measuring strategies foreseen and the sensitivity levels to U/Th that can be reached in this setup., Published by Springer, Heidelberg
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- 2021
16. Tumor suppressor p53 regulates intestinal type 2 immunity
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Wenwei Hu, John J. Ponessa, Lanjing Zhang, Yuhan Zhao, Juan Liu, Zhaohui Feng, Chun Yuan Chang, Mark C. Siracusa, Jianming Wang, Fan Zhou, Huaying Wang, Xue Yang, Juan M. Inclan-Rico, Xuetian Yue, and Ping Xie
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0301 basic medicine ,Science ,General Physics and Astronomy ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,Host-Parasite Interactions ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Immunity ,Cell Line, Tumor ,Intestine, Small ,Parasitic Diseases ,Transcriptional regulation ,Animals ,Humans ,Nippostrongylus brasiliensis ,Tumour-suppressor proteins ,Mice, Knockout ,Innate immunity ,Regulation of gene expression ,Multidisciplinary ,Innate immune system ,Calcium signalling ,Innate lymphoid cell ,Membrane Proteins ,General Chemistry ,biology.organism_classification ,Immunity, Innate ,Gene regulation ,Cell biology ,Eosinophils ,Intestines ,Mice, Inbred C57BL ,Tritrichomonas ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Goblet Cells ,Nippostrongylus ,Tumor Suppressor Protein p53 ,Tuft cell - Abstract
The role of p53 in tumor suppression has been extensively studied and well-established. However, the role of p53 in parasitic infections and the intestinal type 2 immunity is unclear. Here, we report that p53 is crucial for intestinal type 2 immunity in response to the infection of parasites, such as Tritrichomonas muris and Nippostrongylus brasiliensis. Mechanistically, p53 plays a critical role in the activation of the tuft cell-IL-25-type 2 innate lymphoid cell circuit, partly via transcriptional regulation of Lrmp in tuft cells. Lrmp modulates Ca2+ influx and IL-25 release, which are critical triggers of type 2 innate lymphoid cell response. Our results thus reveal a previously unrecognized function of p53 in regulating intestinal type 2 immunity to protect against parasitic infections, highlighting the role of p53 as a guardian of immune integrity., P53 is a well-known tumour suppressor, however its role in intestinal type 2 immunity is currently unclear. Here authors report that during parasitic infections, p53 triggers tuft cell Ca2+ influx and IL-25 release, and shows a regulatory role for p53 in intestinal type 2 immunity via transcriptional regulation of the Lrmp gene.
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- 2021
17. Genealogical relationship inference to identify areas of intensive poaching of the Orange-fronted Parakeet (Eupsittula canicularis)
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Gabriela Padilla-Jacobo, María Guadalupe Zavala-Páramo, Horacio Cano-Camacho, and Tiberio C. Monterrubio-Rico
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0106 biological sciences ,0301 basic medicine ,Genetic diversity ,Illegal trade ,biology ,Cytochrome b ,Wildlife ,Poaching ,Zoology ,Parakeet ,Population expansion ,010603 evolutionary biology ,01 natural sciences ,Nucleotide diversity ,03 medical and health sciences ,Phylogeography ,030104 developmental biology ,QL1-991 ,biology.animal ,Rarefaction (ecology) ,Animal Science and Zoology ,Gene pool ,Species richness - Abstract
Background The Orange-fronted Parakeet (Eupsittula canicularis) is the Mexican psittacine that is most captured for the illegal pet trade. However, as for most wildlife exploited by illegal trade, the genetic diversity that is extracted from species and areas of intensive poaching is unknown. In this study, we analyzed the genetic diversity of 80 E. canicularis parakeets confiscated from the illegal trade and estimated the level of extraction of genetic diversity by poaching using the mitochondrial DNA sequences of cytochrome b (Cytb). In addition, we analyzed the genealogical and haplotypic relationships of the poached parakeets and sampled wild populations in Mexico, as a strategy for identifying the places of origin of poached parakeets. Results Poached parakeets showed high haplotype diversity (Hd = 0.842) and low nucleotide diversity (Pi = 0.00182). Among 22 haplotypes identified, 18 were found exclusively in 37 individuals, while four were detected in the remaining 43 individuals and shared with the wild populations. A rarefaction and extrapolation curve revealed that 240 poached individuals can include up to 47 haplotypes and suggested that the actual haplotype richness of poached parakeets is higher than our analyses indicate. The geographic locations of the four haplotypes shared between poached and wild parakeets ranged from Michoacan to Sinaloa, Mexico. However, the rare haplotypes detected in poached parakeets were derived from a recent genetic expansion of the species that has occurred between the northwest of Michoacan and the coastal region of Colima, Jalisco and southern Nayarit, Mexico. Conclusions Poached parakeets showed high genetic diversity, suggesting high extraction of the genetic pool of the species in central Mexico. Rarefaction and extrapolation analyses suggest that the actual haplotype richness in poached parakeets is higher than reflected by our analyses. The poached parakeets belong mainly to a very diverse genetic group of the species, and their most likely origin is between northern Michoacan and southern Nayarit, Mexico. We found no evidence that poachers included individuals from Central American international trafficking with individuals from Mexico in the sample.
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- 2021
18. Antimicrobial Lessons From a Large Observational Cohort on Intra-abdominal Infections in Intensive Care Units
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Vogelaers, Dirk, Blot, Stijn, Van den Berge, Andries, Montravers, Philippe, Francois, Guy, Labeau, Sonia, Blot, Koen, Deschepper, Mieke, Antonelli, Massimo, Lipman, Jeffrey, Lamrous, Amin, Pereyra, Cecilia, Lipovestky, Fernando, Koulenti, Despoina, De Waele, Jan, Rezende-Neto, Joao, Cardenas, Yenny, Vymazal, Tomas, Fjeldsoee-Nielsen, Hans, Kott, Matthias, Kostoula, Arvaniti, Javeri, Yash, Girardis, Massimo, Einav, Sharon, de Lange, Dylan, Makikado, Luis Daniel Umezawa, Mikstacki, Adam, Paiva, José-Artur, Tomescu, Dana, Gritsan, Alexey, Jovanovic, Bojan, Venkatesan, Kumaresh, Mirkovic, Tomislav, Maseda, Emilio, Dikmen, Yalim, Creagh-Brown, Benedict, Emmerich, Monica, Canale, Mariana, Dietz, Lorena Silvina, Ilutovich, Santiago, Miñope, John Thomas Sanchez, Silva, Ramona Baldomera, Montenegro, Martin Alexis, Martin, Patricio, Saul, Pablo, Chediack, Viviana, Sutton, Giselle, Couce, Rocio, Balasini, Carina, Gonzalez, Susana, Lascar, Florencia Maria, Descotte, Emiliano Jorge, Gumiela, Natalia Soledad, Pino, Carina Alejandra, Cesio, Cristian, Valgolio, Emanuel, Cunto, Eleonora, Dominguez, Cecilia, Nelson, Nydia Funes, Abegao, Esteban Martin, Pozo, Norberto Christian, Bianchi, Luciana, Correger, Enrique, Pastorino, Maria Laura, Miyazaki, Erica Aurora, Grubissich, Nicolas, Garcia, Mariel, Bonetto, Natalia, Quevedo, Noelia Elizabeth, Gomez, Cristina Delia, Queti, Felipe, Estevarena, Luis Gonzalez, Fernandez, Ruben, Santolaya, Ignacio, Grangeat, Sergio Hugo, Doglia, Juan, Zakalik, Graciela, Pellegrini, Carlos, Lloria, Maria Monserrat, Chacon, Mercedes Esteban, Fumale, Mariela, Leguizamon, Mariela, Hidalgo, Irene Beatriz, Tiranti, Roberto Julian, Capponi, Paola, Tita, Agustin, Cardonnet, Luis, Bettini, Lisandro, Ramos, Agñel, Lovesio, Luciano, Miranda, Edith Miriam, Farfan, Angelica Beatriz, Tolosa, Carina, Segura, Lise, Bellocchio, Adelina, Alvarez, Brian, Manzur, Adriana, Lujan, Rodolfo, Fernandez, Natalia, Scarone, Nahuel, Zazu, Alan, Groh, Carina, Fletcher, Jason, Smith, Julie, Azad, Raman, Chavan, Nitin, Wong, Helen, Kol, Mark, Campbell, Lewis, Starr, Therese, Roberts, Brigit, Wibrow, Bradley, Warhurst, Timothy, Chinthamuneedi, Meher, Ferney, Bernal Buitrago, Simon, Marc, De Backer, Daniel, Wittebole, Xavier, De Bels, David, Collin, Vincent, Dams, Karolien, Jorens, Philippe, Dubois, Jasperina, Gunst, Jan, Haentjens, Lionel, De Schryver, Nicolas, Dugernier, Thierry, Rizoli, Sandro, Santillan, Paul, Han, Yi, Biskup, Ewelina, Qu, Changjing, Li, Xinyu, Yu, Tao, Weihua, Lu, Molano-Franco, Daniel, Rojas, José, Oviedo, Juan Mauricio Pardo, Pinilla, Dario, Celis, Edgar, Arias, Mario, Vukovic, Anita, Vudrag, Maja, Belavic, Matija, Zunic, Josip, Kuharic, Janja, Kricka, Irena Bozanic, Filipovic-Grcic, Ina, Tomasevic, Boris, Obraz, Melanija, Bodulica, Bruna, Dohnal, Martin, Malaska, Jan, Kratochvil, Milan, Satinsky, Igor, Schwarz, Peter, Kos, Zdenek, Blahut, Ladislav, Maca, Jan, Protus, Marek, Kieslichová, Eva, Nielsen, Louise Gramstrup, Krogh, Birgitte Marianne, Rivadeneira, Francisco, Morales, Freddy, Mora, José, Orozco, Alexandra Saraguro, MorochoTutillo, Diego Rolando, Vargas, Nelson Remache, Yepez, Estuardo Salgado, Villamagua, Boris, Alsisi, Adel, Fahmy, Abdelraouf, Dupont, Hervé, Lasocki, Sigismond, Paugam-Burtz, Catherine, Foucrier, Arnaud, Nica, Alexandru, Barjon, Geneviève, Mallat, Jihad, Marcotte, Guillaume, Leone, Marc, Duclos, Gary, Burtin, Philippe, Atchade, Enora, Mahjoub, Yazine, Misset, Benoît, Timsit, Jean-François, Dupuis, Claire, Veber, Benoît, Debarre, Matthieu, Collange, Oliver, Pottecher, Julien, Hecketsweiler, Stephane, Fromentin, Mélanie, Tesnière, Antoine, Koch, Christian, Sander, Michael, Eckmann, Christian, Elke, Gunnar, Wrigge, Hermann, Simon, Philipp, Chalkiadaki, Anthoula, Tzanidakis, Charalampos, Pneumatikos, Ioannis, Sertaridou, Eleni, Mastora, Zafiria, Pantazopoulos, Ioannis, Papanikolaou, Metaxia, Papavasilopoulou, Theonymfi, Floros, John, Kolonia, Virginia, Dimopoulos, George, Diakaki, Chryssa, Rallis, Michael, Paridou, Alexandra, Kalogeromitros, Alexandros, Romanou, Vasiliki, Nikolaou, Charikleia, Kounougeri, Katerina, Tsigou, Evdoxia, Psallida, Vasiliki, Karampela, Niki, Mandragos, Konstantinos, Kontoudaki, Eftychia, Pentheroudaki, Alexandra, Farazi-Chongouki, Christos, Karakosta, Agathi, Chouris, Isaac, Radu, Vasiliki, Malliotakis, Polychronis, Kokkini, Sofia, Charalambous, Eliana, Kyritsi, Aikaterini, Koulouras, Vasilios, Papathanakos, Georgios, Nagky, Eva, Lampiri, Clairi, Tsimpoukas, Fotios, Sarakatsanos, Ioannis, Georgakopoulos, Panagiotis, Ravani, Ifigeneia, Prekates, Athanasios, Sakellaridis, Konstantinos, Christopoulos, Christos, Vrettou, Efstratia, Stokkos, Konstantinos, Pentari, Anastasia, Arvaniti, Kostoula, Marmanidou, Kyriaki, Kydona, Christina, Tsoumaropoulos, Georgios, Bitzani, Militisa, Kontou, Paschalina, Voudouris, Antonios, Elli-Nikki, [missing], Flioni, [missing], Antypa, Elli, Chasou, Eleftheria, Anisoglou, Souzana, Papageorgiou, Eirini, Paraforou, Theoniki, Tsioka, Agoritsa, Karathanou, Antigoni, Vakalos, Aristeidis, Shah, Bhagyesh, Thakkar, Chirag, Jain, Nikhilesh, Gurjar, Mohan, Baronia, Arvind, Sathe, Prachee, Kulkarni, Shilpa, Paul, Cherish, Paul, John, Masjedi, Mansoor, Nikandish, Reza, Zand, Farid, Sabetian, Golnar, Mahmoodpoor, Ata, Hashemian, Seyed Mohammadreza, Bala, Miklosh, Flocco, Romeo, Torrente, Sergio, Pota, Vincenzo, Spadaro, Savino, Volta, Carlo, Serafini, Giulia, Boraso, Sabrina, Tiberio, Ivo, Cortegiani, Andrea, Misseri, Giovanni, Barbagallo, Maria, Nicolotti, Davide, Forfori, Francesco, Corradi, Francesco, De Pascale, Gennaro, Pelagalli, Lorella, Brazzi, Luca, Vittone, Ferdinando Giorgio, Russo, Alessandro, Simion, Davide, Cotoia, Antonella, Cinnella, Gilda, Toppin, Patrick, Johnson-Jackson, Roxanne, Hayashi, Yoshiro, Yamamoto, Ryohei, Yasuda, Hideto, Kishihara, Yuki, Shiotsuka, Junji, Sanchez-Hurtado, Luis Alejandro, Tejeda-Huezo, Brigitte, Gorordo, Luis, Ñamendys-Silva, Silvio A., Garcia-Guillen, Francisco J., Martinez, Manuel, Romero-Meja, Erick, Colorado-Dominguez, Ever, van den Oever, Huub, Kalff, Karel Martijn, Vermeijden, Wytze, Cornet, Alexander Daniel, Beck, Oliver, Cimic, Nedim, Dormans, Tom, Bormans, Laura, Bakker, Jan, Van Duijn, Ditty, Bosman, Gerrit, Vos, Piet, Haas, Lenneke, Henein, Akram, Miranda, Ariel M., Malca, Gonzalo Ernesto Gianella, Arroyo-Sanchez, Abel, Misiewska-Kaczur, Agnieszka, Akinyi, Frisch, Czuczwar, Miroslaw, Luczak, Karolina, Sulkowski, Wiktor, Tamowicz, Barbara, Swit, Beata, Baranowski, Bronisław, Smuszkiewicz, Piotr, Trojanowska, Iwona, Rzymski, Stanislaw, Sawinski, Mariusz, Trosiak, Marta, Mikaszewska-Sokolewicz, Malgorzata, Alves, Ricardo, Leal, Dina, Krystopchuk, Andriy, Mendonca, Pedro Muguel Hilario, Pereira, Rui Antunes, de Carvalho, Maria Raquel Lopes Marques, Candeias, Carlos, Molinos, Elena, Ferreira, Amélia, Castro, Guiomar, Pereira, José-Manuel, Santos, Lurdes, Ferreira, Alcina, Pascoalinho, Dulce, Ribeiro, Rosa, Domingos, Guilherme, Gomes, Pedro, Nora, David, Costa, Rui Pedro, Santos, Anabela, Alsheikhly, Ahmed Subhy, Popescu, Mihai, Grigoras, Ioana, Patrascanu, Emilia, Zabolotskikh, Igor, Musaeva, Tatiana, Gaigolnik, Denis, Kulabukhov, Vladimir, Belskiy, Vladislav, Zubareva, Nadezhda, Tribulev, Maxim, Abdelsalam, Ahmed, Aldarsani, Ayman, Al-Khalid, Muhammad, Almekhlafi, Ghaleb, Mandourah, Yasser, Doklestic, Krstina, Velickovic, Jelena, Velickovic, Dejan, Jankovic, Radmilo, Skoric-Jokic, Svetlana, Radovanovic, Dragana, Richards, Guy, Alli, Ahmad, Del Carmen Cordoba Nielfa, Maria, Iniesta, Rafael Sánchez, Martínez, Adela Benítez-Cano, Bernedo, Carlos Garcia, Gil, Santiago Alberto Picos, Nuvials, Xavier, Rello, Jordi, Garcia, Joseba Gonzalez, Peña, Jose Manuel Garcia, Jimenez, Roberto, Herrera, Luis, Barrachina, Laura Galarza, Monzon, Ignacio Catalan, Redondo, Francisco Javier, Villazala, Ruben, Zapata, Diego Fernando Matallana, Lopez, Isabel Maria Villa, Moreno-Gonzalez, Gabriel, Lopez-Delgado, Juan Carlos, Marin, Jorge Solera, Sanchez-Zamora, Purificacion, Vidal, Montserrat Vallverdú, González, Jesús Flores, Salinas, Irene, Hermosa, Cecilia, Martinez-Sagasti, Fernando, Domingo-Marín, Sara, Victorino, Johanna Abril, Garcia-Alvarez, Raquel, Calleja, Pablo López-Arcas, de la Torre-Prados, Maria-Victoria, Vidal-Cortes, Pablo, Del Río-Carbajo, Lorena, Izura, Javier, Minguez, Victoria, Alvarez, Josep Trenado, Prous, Anna Parera, Paz, Daniel, Roche-Campo, Ferran, Aguilar, Gerardo, Belda, Javier, Rico-Feijoo, Jesus, Aldecoa, Cesat, Zalba-Etayo, Begoña, Lang, Martin, Dullenkopf, Alexander, Trongtrakul, Konlawij, Chtsomkasem, Anusang, Akbaş, Türkay, Unal, Mustafa Necmettin, Ozcelik, Menekse, Gumus, Ayca, Ramazanoglu, Atilla, Memis, Dilek, Mehmet, Inal, Urkmez, Seval, Ozgultekin, Asu, Demirkiran, Oktay, Aslan, Nesrin Ahu, Kizilaslan, Deniz, Kahveci, Ferda, Ünlü, Nurdan, Ozkan, Zeynep, Kaye, Callum, Jansen, Jan, O’Neill, Orla, Nutt, Christopher, Jha, Rajeev, Hooker, Nicolas, Grecu, Irina, Petridou, Christina, Shyamsundar, Murali, McNamee, Lia, Trinder, John, Hagan, Samantha, Kelly, Catriona, Silversides, Jonathon, Groba, Casiano Barrera, Boyd, Owen, Bhowmick, Kaushik, Humphreys, Sally, Summers, Charlotte, Polgarova, Petra, Margarson, Michael, Dickens, Justin, Pearson, Suzanne, Chinery, Elaine, Hemmings, Noel, O’Kane, Sinead, Austin, Pauline, Cole, Stephen, Plowright, Catherine, Box, Roberta, Wright, Christopher, Young, Lorna, Creagh-Brown, Ben, Montague, Laura, Parker, Robert, Morton, Ben, Ostermann, Marlies, Bilinska, Julia, Rose, Bernd Oliver, Reece-Anthony, Rosie, Ryan, Christine, Hamilton, Mark, Hopkins, Philip, Wendon, Julia, Brescia, Giovanni, Ijaz, Nazia, Wood, James, George, Michelle, Toth-Tarsoly, Piroska, Yates, Bryan, Armstrong, Maureen, Scott, Carmen, Boyd, Christine, Szakmany, Tamas, Rees, David, Pulak, Paul, Coggon, Mandy, Saha, Bhaskar, Kent, Linda, Gibson, Bethan, Camsooksai, Julie, Reschreiter, Henrik, Morgan, Pat, Sangaralingham, Sivatharshini, Lowe, Alastair, Vondras, Petr, Jamadarkhana, Sunil, Cruz, Carina, Bhandary, Rakesh, Hersey, Peter, Furneval, Julie, Innes, Richard, Doble, Patricia, Attwood, Ben, Parsons, Penny, Page, Valerie, Zhao, Xiaobei, Dalton, Julian, Hegazy, Mohammed, Awad, Yasser, Naylor, Douglas, Naylor, Amanda, Lee, Sarah, Brevard, Sidney, Davis, Noelle, for the Abdominal Sepsis Study (‘AbSeS’) Group on behalf of the Trials Group of the European Society of Intensive Care Medicine, [missing], Vogelaers D., Blot S., Van den Berge A., Montravers P., Francois G., Labeau S., Blot K., Deschepper M., Antonelli M., Lipman J., Lamrous A., Pereyra C., Lipovestky F., Koulenti D., De Waele J., Rezende-Neto J., Cardenas Y., Vymazal T., Fjeldsoee-Nielsen H., Kott M., Kostoula A., Javeri Y., Girardis M., Einav S., de Lange D., Makikado L.D.U., Mikstacki A., Paiva J.-A., Tomescu D., Gritsan A., Jovanovic B., Venkatesan K., Mirkovic T., Maseda E., Dikmen Y., Creagh-Brown B., Emmerich M., Canale M., Dietz L.S., Ilutovich S., Minope J.T.S., Silva R.B., Montenegro M.A., Martin P., Saul P., Chediack V., Sutton G., Couce R., Balasini C., Gonzalez S., Lascar F.M., Descotte E.J., Gumiela N.S., Pino C.A., Cesio C., Valgolio E., Cunto E., Dominguez C., Nelson N.F., Abegao E.M., Pozo N.C., Bianchi L., Correger E., Pastorino M.L., Miyazaki E.A., Grubissich N., Garcia M., Bonetto N., Quevedo N.E., Gomez C.D., Queti F., Estevarena L.G., Fernandez R., Santolaya I., Grangeat S.H., Doglia J., Zakalik G., Pellegrini C., Lloria M.M., Chacon M.E., Fumale M., Leguizamon M., Hidalgo I.B., Tiranti R.J., Capponi P., Tita A., Cardonnet L., Bettini L., Ramos A., Lovesio L., Miranda E.M., Farfan A.B., Tolosa C., Segura L., Bellocchio A., Alvarez B., Manzur A., Lujan R., Fernandez N., Scarone N., Zazu A., Groh C., Fletcher J., Smith J., Azad R., Chavan N., Wong H., Kol M., Campbell L., Starr T., Roberts B., Wibrow B., Warhurst T., Chinthamuneedi M., Ferney B.B., Simon M., De Backer D., Wittebole X., De Bels D., Collin V., Dams K., Jorens P., Dubois J., Gunst J., Haentjens L., De Schryver N., Dugernier T., Rizoli S., Santillan P., Han Y., Biskup E., Qu C., Li X., Yu T., Weihua L., Molano-Franco D., Rojas J., Oviedo J.M.P., Pinilla D., Celis E., Arias M., Vukovic A., Vudrag M., Belavic M., Zunic J., Kuharic J., Kricka I.B., Filipovic-Grcic I., Tomasevic B., Obraz M., Bodulica B., Dohnal M., Malaska J., Kratochvil M., Satinsky I., Schwarz P., Kos Z., Blahut L., Maca J., Protus M., Kieslichova E., Nielsen L.G., Krogh B.M., Rivadeneira F., Morales F., Mora J., Orozco A.S., MorochoTutillo D.R., Vargas N.R., Yepez E.S., Villamagua B., Alsisi A., Fahmy A., Dupont H., Lasocki S., Paugam-Burtz C., Foucrier A., Nica A., Barjon G., Mallat J., Marcotte G., Leone M., Duclos G., Burtin P., Atchade E., Mahjoub Y., Misset B., Timsit J.-F., Dupuis C., Veber B., Debarre M., Collange O., Pottecher J., Hecketsweiler S., Fromentin M., Tesniere A., Koch C., Sander M., Eckmann C., Elke G., Wrigge H., Simon P., Chalkiadaki A., Tzanidakis C., Pneumatikos I., Sertaridou E., Mastora Z., Pantazopoulos I., Papanikolaou M., Papavasilopoulou T., Floros J., Kolonia V., Dimopoulos G., Diakaki C., Rallis M., Paridou A., Kalogeromitros A., Romanou V., Nikolaou C., Kounougeri K., Tsigou E., Psallida V., Karampela N., Mandragos K., Kontoudaki E., Pentheroudaki A., Farazi-Chongouki C., Karakosta A., Chouris I., Radu V., Malliotakis P., Kokkini S., Charalambous E., Kyritsi A., Koulouras V., Papathanakos G., Nagky E., Lampiri C., Tsimpoukas F., Sarakatsanos I., Georgakopoulos P., Ravani I., Prekates A., Sakellaridis K., Christopoulos C., Vrettou E., Stokkos K., Pentari A., Arvaniti K., Marmanidou K., Kydona C., Tsoumaropoulos G., Bitzani M., Kontou P., Voudouris A., Elli-Nikki, Flioni, Antypa E., Chasou E., Anisoglou S., Papageorgiou E., Paraforou T., Tsioka A., Karathanou A., Vakalos A., Shah B., Thakkar C., Jain N., Gurjar M., Baronia A., Sathe P., Kulkarni S., Paul C., Paul J., Masjedi M., Nikandish R., Zand F., Sabetian G., Mahmoodpoor A., Hashemian S.M., Bala M., Flocco R., Torrente S., Pota V., Spadaro S., Volta C., Serafini G., Boraso S., Tiberio I., Cortegiani A., Misseri G., Barbagallo M., Nicolotti D., Forfori F., Corradi F., De Pascale G., Pelagalli L., Brazzi L., Vittone F.G., Russo A., Simion D., Cotoia A., Cinnella G., Toppin P., Johnson-Jackson R., Hayashi Y., Yamamoto R., Yasuda H., Kishihara Y., Shiotsuka J., Sanchez-Hurtado L.A., Tejeda-Huezo B., Gorordo L., Namendys-Silva S.A., Garcia-Guillen F.J., Martinez M., Romero-Meja E., Colorado-Dominguez E., van den Oever H., Kalff K.M., Vermeijden W., Cornet A.D., Beck O., Cimic N., Dormans T., Bormans L., Bakker J., Van Duijn D., Bosman G., Vos P., Haas L., Henein A., Miranda A.M., Malca G.E.G., Arroyo-Sanchez A., Misiewska-Kaczur A., Akinyi F., Czuczwar M., Luczak K., Sulkowski W., Tamowicz B., Swit B., Baranowski B., Smuszkiewicz P., Trojanowska I., Rzymski S., Sawinski M., Trosiak M., Mikaszewska-Sokolewicz M., Alves R., Leal D., Krystopchuk A., Mendonca P.M.H., Pereira R.A., de Carvalho M.R.L.M., Candeias C., Molinos E., Ferreira A., Castro G., Pereira J.-M., Santos L., Pascoalinho D., Ribeiro R., Domingos G., Gomes P., Nora D., Costa R.P., Santos A., Alsheikhly A.S., Popescu M., Grigoras I., Patrascanu E., Zabolotskikh I., Musaeva T., Gaigolnik D., Kulabukhov V., Belskiy V., Zubareva N., Tribulev M., Abdelsalam A., Aldarsani A., Al-Khalid M., Almekhlafi G., Mandourah Y., Doklestic K., Velickovic J., Velickovic D., Jankovic R., Skoric-Jokic S., Radovanovic D., Richards G., Alli A., Del Carmen Cordoba Nielfa M., Iniesta R.S., Martinez A.B.-C., Bernedo C.G., Gil S.A.P., Nuvials X., Rello J., Garcia J.G., Pena J.M.G., Jimenez R., Herrera L., Barrachina L.G., Monzon I.C., Redondo F.J., Villazala R., Zapata D.F.M., Lopez I.M.V., Moreno-Gonzalez G., Lopez-Delgado J.C., Marin J.S., Sanchez-Zamora P., Vidal M.V., Gonzalez J.F., Salinas I., Hermosa C., Martinez-Sagasti F., Domingo-Marin S., Victorino J.A., Garcia-Alvarez R., Calleja P.L.-A., de la Torre-Prados M.-V., Vidal-Cortes P., Del Rio-Carbajo L., Izura J., Minguez V., Alvarez J.T., Prous A.P., Paz D., Roche-Campo F., Aguilar G., Belda J., Rico-Feijoo J., Aldecoa C., Zalba-Etayo B., Lang M., Dullenkopf A., Trongtrakul K., Chtsomkasem A., Akbas T., Unal M.N., Ozcelik M., Gumus A., Ramazanoglu A., Memis D., Mehmet I., Urkmez S., Ozgultekin A., Demirkiran O., Aslan N.A., Kizilaslan D., Kahveci F., Unlu N., Ozkan Z., Kaye C., Jansen J., O'Neill O., Nutt C., Jha R., Hooker N., Grecu I., Petridou C., Shyamsundar M., McNamee L., Trinder J., Hagan S., Kelly C., Silversides J., Groba C.B., Boyd O., Bhowmick K., Humphreys S., Summers C., Polgarova P., Margarson M., Dickens J., Pearson S., Chinery E., Hemmings N., O'Kane S., Austin P., Cole S., Plowright C., Box R., Wright C., Young L., Montague L., Parker R., Morton B., Ostermann M., Bilinska J., Rose B.O., Reece-Anthony R., Ryan C., Hamilton M., Hopkins P., Wendon J., Brescia G., Ijaz N., Wood J., George M., Toth-Tarsoly P., Yates B., Armstrong M., Scott C., Boyd C., Szakmany T., Rees D., Pulak P., Coggon M., Saha B., Kent L., Gibson B., Camsooksai J., Reschreiter H., Morgan P., Sangaralingham S., Lowe A., Vondras P., Jamadarkhana S., Cruz C., Bhandary R., Hersey P., Furneval J., Innes R., Doble P., Attwood B., Parsons P., Page V., Zhao X., Dalton J., Hegazy M., Awad Y., Naylor D., Naylor A., Lee S., Brevard S., Davis N., UCL - SSS/IREC/MEDA - Pôle de médecine aiguë, UCL - (SLuc) Service de soins intensifs, Vogelaers, D., Blot, S., Van den Berge, A., Montravers, P., Francois, G., Labeau, S., Blot, K., Deschepper, M., Antonelli, M., Lipman, J., Lamrous, A., Pereyra, C., Lipovestky, F., Koulenti, D., De Waele, J., Rezende-Neto, J., Cardenas, Y., Vymazal, T., Fjeldsoee-Nielsen, H., Kott, M., Kostoula, A., Javeri, Y., Girardis, M., Einav, S., de Lange, D., Makikado, L. D. U., Mikstacki, A., Paiva, J. -A., Tomescu, D., Gritsan, A., Jovanovic, B., Venkatesan, K., Mirkovic, T., Maseda, E., Dikmen, Y., Creagh-Brown, B., Emmerich, M., Canale, M., Dietz, L. S., Ilutovich, S., Minope, J. T. S., Silva, R. B., Montenegro, M. A., Martin, P., Saul, P., Chediack, V., Sutton, G., Couce, R., Balasini, C., Gonzalez, S., Lascar, F. M., Descotte, E. J., Gumiela, N. S., Pino, C. A., Cesio, C., Valgolio, E., Cunto, E., Dominguez, C., Nelson, N. F., Abegao, E. M., Pozo, N. C., Bianchi, L., Correger, E., Pastorino, M. L., Miyazaki, E. A., Grubissich, N., Garcia, M., Bonetto, N., Quevedo, N. E., Gomez, C. D., Queti, F., Estevarena, L. G., Fernandez, R., Santolaya, I., Grangeat, S. H., Doglia, J., Zakalik, G., Pellegrini, C., Lloria, M. M., Chacon, M. E., Fumale, M., Leguizamon, M., Hidalgo, I. B., Tiranti, R. J., Capponi, P., Tita, A., Cardonnet, L., Bettini, L., Ramos, A., Lovesio, L., Miranda, E. M., Farfan, A. B., Tolosa, C., Segura, L., Bellocchio, A., Alvarez, B., Manzur, A., Lujan, R., Fernandez, N., Scarone, N., Zazu, A., Groh, C., Fletcher, J., Smith, J., Azad, R., Chavan, N., Wong, H., Kol, M., Campbell, L., Starr, T., Roberts, B., Wibrow, B., Warhurst, T., Chinthamuneedi, M., Ferney, B. B., Simon, M., De Backer, D., Wittebole, X., De Bels, D., Collin, V., Dams, K., Jorens, P., Dubois, J., Gunst, J., Haentjens, L., De Schryver, N., Dugernier, T., Rizoli, S., Santillan, P., Han, Y., Biskup, E., Qu, C., Li, X., Yu, T., Weihua, L., Molano-Franco, D., Rojas, J., Oviedo, J. M. P., Pinilla, D., Celis, E., Arias, M., Vukovic, A., Vudrag, M., Belavic, M., Zunic, J., Kuharic, J., Kricka, I. B., Filipovic-Grcic, I., Tomasevic, B., Obraz, M., Bodulica, B., Dohnal, M., Malaska, J., Kratochvil, M., Satinsky, I., Schwarz, P., Kos, Z., Blahut, L., Maca, J., Protus, M., Kieslichova, E., Nielsen, L. G., Krogh, B. M., Rivadeneira, F., Morales, F., Mora, J., Orozco, A. S., Morochotutillo, D. R., Vargas, N. R., Yepez, E. S., Villamagua, B., Alsisi, A., Fahmy, A., Dupont, H., Lasocki, S., Paugam-Burtz, C., Foucrier, A., Nica, A., Barjon, G., Mallat, J., Marcotte, G., Leone, M., Duclos, G., Burtin, P., Atchade, E., Mahjoub, Y., Misset, B., Timsit, J. -F., Dupuis, C., Veber, B., Debarre, M., Collange, O., Pottecher, J., Hecketsweiler, S., Fromentin, M., Tesniere, A., Koch, C., Sander, M., Eckmann, C., Elke, G., Wrigge, H., Simon, P., Chalkiadaki, A., Tzanidakis, C., Pneumatikos, I., Sertaridou, E., Mastora, Z., Pantazopoulos, I., Papanikolaou, M., Papavasilopoulou, T., Floros, J., Kolonia, V., Dimopoulos, G., Diakaki, C., Rallis, M., Paridou, A., Kalogeromitros, A., Romanou, V., Nikolaou, C., Kounougeri, K., Tsigou, E., Psallida, V., Karampela, N., Mandragos, K., Kontoudaki, E., Pentheroudaki, A., Farazi-Chongouki, C., Karakosta, A., Chouris, I., Radu, V., Malliotakis, P., Kokkini, S., Charalambous, E., Kyritsi, A., Koulouras, V., Papathanakos, G., Nagky, E., Lampiri, C., Tsimpoukas, F., Sarakatsanos, I., Georgakopoulos, P., Ravani, I., Prekates, A., Sakellaridis, K., Christopoulos, C., Vrettou, E., Stokkos, K., Pentari, A., Arvaniti, K., Marmanidou, K., Kydona, C., Tsoumaropoulos, G., Bitzani, M., Kontou, P., Voudouris, A., Elli-Nikki, Flioni, Antypa, E., Chasou, E., Anisoglou, S., Papageorgiou, E., Paraforou, T., Tsioka, A., Karathanou, A., Vakalos, A., Shah, B., Thakkar, C., Jain, N., Gurjar, M., Baronia, A., Sathe, P., Kulkarni, S., Paul, C., Paul, J., Masjedi, M., Nikandish, R., Zand, F., Sabetian, G., Mahmoodpoor, A., Hashemian, S. M., Bala, M., Flocco, R., Torrente, S., Pota, V., Spadaro, S., Volta, C., Serafini, G., Boraso, S., Tiberio, I., Cortegiani, A., Misseri, G., Barbagallo, M., Nicolotti, D., Forfori, F., Corradi, F., De Pascale, G., Pelagalli, L., Brazzi, L., Vittone, F. G., Russo, A., Simion, D., Cotoia, A., Cinnella, G., Toppin, P., Johnson-Jackson, R., Hayashi, Y., Yamamoto, R., Yasuda, H., Kishihara, Y., Shiotsuka, J., Sanchez-Hurtado, L. A., Tejeda-Huezo, B., Gorordo, L., Namendys-Silva, S. A., Garcia-Guillen, F. J., Martinez, M., Romero-Meja, E., Colorado-Dominguez, E., van den Oever, H., Kalff, K. M., Vermeijden, W., Cornet, A. D., Beck, O., Cimic, N., Dormans, T., Bormans, L., Bakker, J., Van Duijn, D., Bosman, G., Vos, P., Haas, L., Henein, A., Miranda, A. M., Malca, G. E. G., Arroyo-Sanchez, A., Misiewska-Kaczur, A., Akinyi, F., Czuczwar, M., Luczak, K., Sulkowski, W., Tamowicz, B., Swit, B., Baranowski, B., Smuszkiewicz, P., Trojanowska, I., Rzymski, S., Sawinski, M., Trosiak, M., Mikaszewska-Sokolewicz, M., Alves, R., Leal, D., Krystopchuk, A., Mendonca, P. M. H., Pereira, R. A., de Carvalho, M. R. L. M., Candeias, C., Molinos, E., Ferreira, A., Castro, G., Pereira, J. -M., Santos, L., Pascoalinho, D., Ribeiro, R., Domingos, G., Gomes, P., Nora, D., Costa, R. P., Santos, A., Alsheikhly, A. S., Popescu, M., Grigoras, I., Patrascanu, E., Zabolotskikh, I., Musaeva, T., Gaigolnik, D., Kulabukhov, V., Belskiy, V., Zubareva, N., Tribulev, M., Abdelsalam, A., Aldarsani, A., Al-Khalid, M., Almekhlafi, G., Mandourah, Y., Doklestic, K., Velickovic, J., Velickovic, D., Jankovic, R., Skoric-Jokic, S., Radovanovic, D., Richards, G., Alli, A., Del Carmen Cordoba Nielfa, M., Iniesta, R. S., Martinez, A. B. -C., Bernedo, C. G., Gil, S. A. P., Nuvials, X., Rello, J., Garcia, J. G., Pena, J. M. G., Jimenez, R., Herrera, L., Barrachina, L. G., Monzon, I. C., Redondo, F. J., Villazala, R., Zapata, D. F. M., Lopez, I. M. V., Moreno-Gonzalez, G., Lopez-Delgado, J. C., Marin, J. S., Sanchez-Zamora, P., Vidal, M. V., Gonzalez, J. F., Salinas, I., Hermosa, C., Martinez-Sagasti, F., Domingo-Marin, S., Victorino, J. A., Garcia-Alvarez, R., Calleja, P. L. -A., de la Torre-Prados, M. -V., Vidal-Cortes, P., Del Rio-Carbajo, L., Izura, J., Minguez, V., Alvarez, J. T., Prous, A. P., Paz, D., Roche-Campo, F., Aguilar, G., Belda, J., Rico-Feijoo, J., Aldecoa, C., Zalba-Etayo, B., Lang, M., Dullenkopf, A., Trongtrakul, K., Chtsomkasem, A., Akbas, T., Unal, M. N., Ozcelik, M., Gumus, A., Ramazanoglu, A., Memis, D., Mehmet, I., Urkmez, S., Ozgultekin, A., Demirkiran, O., Aslan, N. A., Kizilaslan, D., Kahveci, F., Unlu, N., Ozkan, Z., Kaye, C., Jansen, J., O'Neill, O., Nutt, C., Jha, R., Hooker, N., Grecu, I., Petridou, C., Shyamsundar, M., Mcnamee, L., Trinder, J., Hagan, S., Kelly, C., Silversides, J., Groba, C. B., Boyd, O., Bhowmick, K., Humphreys, S., Summers, C., Polgarova, P., Margarson, M., Dickens, J., Pearson, S., Chinery, E., Hemmings, N., O'Kane, S., Austin, P., Cole, S., Plowright, C., Box, R., Wright, C., Young, L., Montague, L., Parker, R., Morton, B., Ostermann, M., Bilinska, J., Rose, B. O., Reece-Anthony, R., Ryan, C., Hamilton, M., Hopkins, P., Wendon, J., Brescia, G., Ijaz, N., Wood, J., George, M., Toth-Tarsoly, P., Yates, B., Armstrong, M., Scott, C., Boyd, C., Szakmany, T., Rees, D., Pulak, P., Coggon, M., Saha, B., Kent, L., Gibson, B., Camsooksai, J., Reschreiter, H., Morgan, P., Sangaralingham, S., Lowe, A., Vondras, P., Jamadarkhana, S., Cruz, C., Bhandary, R., Hersey, P., Furneval, J., Innes, R., Doble, P., Attwood, B., Parsons, P., Page, V., Zhao, X., Dalton, J., Hegazy, M., Awad, Y., Naylor, D., Naylor, A., Lee, S., Brevard, S., and Davis, N.
- Subjects
Drug Resistance ,medicine.disease_cause ,Severity of Illness Index ,law.invention ,0302 clinical medicine ,ENTEROBACTERIACEAE ,law ,Drug Resistance, Multiple, Bacterial ,Medicine and Health Sciences ,Pharmacology (medical) ,Cross Infection ,biology ,Bacterial ,Antimicrobial ,Intensive care unit ,Anti-Bacterial Agents ,Community-Acquired Infections ,Europe ,Intensive Care Units ,Critical Illness ,Humans ,Intraabdominal Infections ,Microbial Sensitivity Tests ,Peritonitis ,Sepsis ,ESCHERICHIA-COLI ,030220 oncology & carcinogenesis ,KLEBSIELLA-PNEUMONIAE ,BLOOD-STREAM INFECTIONS ,PYELONEPHRITIS ,Multiple ,medicine.medical_specialty ,Enterococcus faecalis ,NO ,03 medical and health sciences ,Intra‑abdominal Infections ,Antibiotic resistance ,FOOD ,Intensive care ,Internal medicine ,medicine ,FLUOROQUINOLONE RESISTANCE ,Pseudomonas aeruginosa ,business.industry ,Septic shock ,MORTALITY ,biology.organism_classification ,medicine.disease ,RISK-FACTORS ,business ,030217 neurology & neurosurgery ,Enterococcus faecium - Abstract
Severe intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by disease-specific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. In this study, we report on resistance profiles of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Enterococcus faecium in distinct European geographic regions based on an observational cohort study on intra-abdominal infections in intensive care unit (ICU) patients. Resistance against aminopenicillins, fluoroquinolones, and third-generation cephalosporins in E. coli, K. pneumoniae and P. aeruginosa is problematic, as is carbapenem-resistance in the latter pathogen. For E. coli and K. pneumoniae, resistance is mainly an issue in Central Europe, Eastern and South-East Europe, and Southern Europe, while resistance in P. aeruginosa is additionally problematic in Western Europe. Vancomycin-resistance in E. faecalis is of lesser concern but requires vigilance in E. faecium in Central and Eastern and South-East Europe. In the subcohort of patients with secondary peritonitis presenting with either sepsis or septic shock, the appropriateness of empiric antimicrobial therapy was not associated with mortality. In contrast, failure of source control was strongly associated with mortality. The relevance of these new insights for future recommendations regarding empiric antimicrobial therapy in intra-abdominal infections is discussed.
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- 2021
19. Synthesis, structure and midkine binding of chondroitin sulfate oligosaccharide analogues
- Author
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Myriam Torres-Rico, Susana Maza, José L. de Paz, Pedro M. Nieto, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Torres-Rico, Myriam, Paz, José L. de, Nieto, Pedro M., Torres-Rico, Myriam [0000-0002-5222-2778], Paz, José L. de [0000-0002-8555-2271], and Nieto, Pedro M. [0000-0002-4074-9011]
- Subjects
Midkine ,chemistry.chemical_classification ,Binding Sites ,Magnetic Resonance Spectroscopy ,biology ,Chemistry ,Stereochemistry ,Chondroitin Sulfates ,Organic Chemistry ,Oligosaccharides ,Oligosaccharide ,Glucuronic acid ,Biochemistry ,Molecular dynamics ,chemistry.chemical_compound ,Glucose ,Saturation transfer ,Carbohydrate Conformation ,biology.protein ,Humans ,Carboxylate ,Chondroitin sulfate ,Physical and Theoretical Chemistry ,Fluorescence anisotropy - Abstract
The preparation of chondroitin sulfate (CS) oligosaccharide mimetics, more easily synthesized than natural sequences, is a highly interesting task because these compounds pave the way for modulation of the biological processes in which CS is involved. Herein, we report the synthesis of CS type E analogues which present easily accessible glucose units instead of glucuronic acid (GlcA) moieties. NMR experiments and molecular dynamics simulations showed that the 3D structure of these compounds is similar to the structure of the natural CS-E oligosaccharides. In addition, fluorescence polarization (FP) and saturation transfer difference NMR (STD-NMR) experiments revealed that the synthesized CS-like derivatives were able to interact with midkine, a model heparin-binding growth factor, suggesting that the presence of the GlcA carboxylate groups is not essential for the binding. Overall, our results indicate that the synthesized glucose-containing oligosaccharides can be considered as functional and structural CS mimetics
- Published
- 2021
20. In Vitro Bedaquiline and Clofazimine Susceptibility Testing in Mycobacterium abscessus
- Author
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Bettina Schulthess, Fatma Nur Akdoğan Kittana, Rico Hömke, Peter Sander, University of Zurich, and Schulthess, Bettina
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Pharmacology ,Infectious Diseases ,3004 Pharmacology ,Susceptibility ,10179 Institute of Medical Microbiology ,570 Life sciences ,biology ,2736 Pharmacology (medical) ,Pharmacology (medical) ,610 Medicine & health ,2725 Infectious Diseases - Abstract
Bedaquiline and clofazimine are increasingly used to treat infections with Mycobacterium abscessus . We determined distributions of MICs by broth microdilution for bedaquiline and clofazimine for 61 M. abscessus clinical isolates using different media and incubation times. We show that incubation time and growth media critically influence the MIC. Our data will aid in defining future clinical breakpoints for in vitro susceptibility testing for bedaquiline and clofazimine in M. abscessus .
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- 2022
21. Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities
- Author
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Chanpen Chanchao, Preecha Phuwapraisirisan, Phanthiwa Khongkarat, and Rico Ramadhan
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Antioxidant ,partitioned extract ,medicine.medical_treatment ,naringenin ,mimosa ,Plant Science ,01 natural sciences ,Mimosa pigra ,0404 agricultural biotechnology ,Anti tyrosinase ,medicine ,biology ,Traditional medicine ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,bee pollen ,biology.organism_classification ,040401 food science ,0104 chemical sciences ,Bioguided fractionation ,QL1-991 ,Insect Science ,Bee pollen ,chromatography ,Zoology - Abstract
Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).
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- 2021
22. Case Series of Laboratory-Associated Zika Virus Disease, United States, 2016–2019
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Catherine M. Brown, Betsy A. Schroeder, Cherie Drenzek, Lea Heberlein-Larson, Marc Fischer, Glen R. Gallagher, Matthew Osborne, Krystal L Mason, Shawna Stuck, Julie Gabel, Jennifer Fiddner, Susan L. Hills, Danielle Stanek, Kayleigh Sandhu, Andrea Morrison, and Edhelene Rico
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Microbiology (medical) ,Zika virus disease ,Epidemiology ,viruses ,030231 tropical medicine ,Infectious and parasitic diseases ,RC109-216 ,Case Series of Laboratory-Associated Zika Virus Disease, United States, 2016–2019 ,Zika virus ,mosquito-borne diseases ,03 medical and health sciences ,Biosafety ,0302 clinical medicine ,flavivirus ,vector-borne diseases ,medicine ,needlestick ,Humans ,030212 general & internal medicine ,occupational ,Laboratory research ,biology ,business.industry ,Transmission (medicine) ,Zika Virus Infection ,Research ,Diagnostic test ,Zika Virus ,biology.organism_classification ,medicine.disease ,Virology ,United States ,zoonoses ,Flavivirus ,Infectious Diseases ,Synopsis ,Medicine ,Americas ,business ,Laboratories ,laboratory - Abstract
Zika virus diagnostic testing and laboratory research increased considerably when Zika virus began spreading through the Americas in 2015, increasing the risk for potential Zika virus exposure of laboratory workers and biomedical researchers. We report 4 cases of laboratory-associated Zika virus disease in the United States during 2016-2019. Of these, 2 were associated with needlestick injuries; for the other 2 cases, the route of transmission was undetermined. In laboratories in which work with Zika virus is performed, good laboratory biosafety practices must be implemented and practiced to reduce the risk for infection among laboratory personnel.
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- 2021
23. Characterization of the secretory profile and exosomes of limbal stem cells in the canine species
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Viviana Fernández, José Becerra, Antonio J. Villatoro, Cristina Alcoholado, Gustavo Rico, María del Carmen Martín-Astorga, [Villatoro,AJ, Alcoholado,C, Martín-Astorga,MC, Rico,G, Becerra,J] Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Málaga, IBIMA, Málaga, Spain. [Villatoro,AJ, Fernández,V] Instituto de Immunología Clínica y Terapia Celular (IMMUNESTEM), Málaga, Spain. [Alcoholado,C] Biomedicine Research Institute of Malaga (IBIMA), Campus Universitario Teatinos, Málaga, Spain. [Alcoholado,C, Rico,G, Becerra,J] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Malaga, Spain. [Rico,G, Becerra,J] Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain., and GR was supported by the RD16/0011/ 0022 grant of the Spanish Network on Cell Therapy (TerCel). AV and VF were supported by the Contract Aljama Gestión SL-University of Malaga (8.06/5.02.5013). MM was supported by the UMA18-FEDERJA-133 grant of the Operational programme FEDER Andalucía 2014-2020. CA was supported by the Promotion of Youth Employment and implementation of the Youth Guarantee in R&D&I, 2018 (PEJ2018-004785-A) grant of the Ministry of Science, Innovation and University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. VI National R&D&I Plan 2008– 2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, Instituto de Salud Carlos III with assistance from the European Regional Development Fund and Regional Government of Andalusia (Junta de Andalucia, PAIDI (BIO217), provided support in the form of materials, but did not have any additional role in the study design, data Cover Letter collection and analysis, decision to publish, or preparation of the manuscript.
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0301 basic medicine ,Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation [Medical Subject Headings] ,Proteomics ,Vascular Endothelial Growth Factor A ,MTS assay ,Cell ,Cell Culture Techniques ,Stem cells ,Exosomes ,Biochemistry ,Exosomas ,Cornea ,0302 clinical medicine ,Animal Cells ,Organisms::Eukaryota::Animals [Medical Subject Headings] ,Medicine and Health Sciences ,Enzyme assays ,Colorimetric assays ,Stem Cell Niche ,Bioassays and physiological analysis ,Cells, Cultured ,Chemokine CCL2 ,Corneal epithelium ,Connective Tissue Cells ,education.field_of_study ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Carnivora::Canidae::Dogs [Medical Subject Headings] ,Multidisciplinary ,Ecology ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-10 [Medical Subject Headings] ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines, CXC::Interleukin-8 [Medical Subject Headings] ,Stem Cells ,Cell Differentiation ,Cell biology ,Interleukin-10 ,medicine.anatomical_structure ,Connective Tissue ,Medicine ,Ecological Niches ,Stem cell ,Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Cytoplasmic Vesicles::Transport Vesicles::Exosomes [Medical Subject Headings] ,Cellular Structures and Organelles ,Anatomy ,Cellular Types ,Chemicals and Drugs::Biological Factors::Biological Markers::Biomarkers, Pharmacological [Medical Subject Headings] ,Research Article ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Angiogenic Proteins::Vascular Endothelial Growth Factors::Vascular Endothelial Growth Factor A [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Culture Techniques [Medical Subject Headings] ,Células madre ,Corneal diseases ,Science ,Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics [Medical Subject Headings] ,Ocular Anatomy ,Population ,Epithelium, corneal ,Biology ,Limbus Corneae ,03 medical and health sciences ,Dogs ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines, CC::Chemokine CCL20 [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media [Medical Subject Headings] ,Ocular System ,medicine ,Animals ,Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea::Limbus Corneae [Medical Subject Headings] ,Vesicles ,education ,Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings] ,Perros ,Cell Proliferation ,Proteomic Profile ,Ecology and Environmental Sciences ,Interleukin-8 ,Biology and Life Sciences ,Proteins ,Cell Biology ,Fibroblasts ,Microvesicles ,In vitro ,Fibroblastos ,Culture Media ,Enfermedades de la cornea ,Research and analysis methods ,Cytoskeletal Proteins ,030104 developmental biology ,Biological Tissue ,Anatomy::Cells::Stem Cells [Medical Subject Headings] ,Anatomy::Cells::Cells, Cultured [Medical Subject Headings] ,Biochemical analysis ,030221 ophthalmology & optometry ,Biomarkers ,Epitelio corneal - Abstract
Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of the corneal epithelium. Their deficiency is responsible for the conjunctivization of the cornea that is seen in different ocular pathologies, both in humans and in the canine species. The canine species represents an interesting preclinical animal model in ocular surface pathologies. However, the role of LSCs in physiological and pathological conditions in canine species is not well understood. Our objective was to characterize for the first time the soluble factors and the proteomic profile of the secretome and exosomes of canine LSCs (cLSCs). In addition, given the important role that fibroblasts play in the repair of the ocular surface, we evaluated the influence of the secretome and exosomes of cLSCs on their proliferation in vitro. Our results demonstrated a secretory profile of cLSCs with high concentrations of MCP-1, IL-8, VEGF-A, and IL-10, as well as significant production of exosomes. Regarding the proteomic profile, 646 total proteins in the secretome and 356 in exosomes were involved in different biological processes. Functionally, the cLSC secretome showed an inhibitory effect on the proliferation of fibroblasts in vitro, which the exosomes did not. These results open the door to new studies on the possible use of the cLSC secretome or some of its components to treat certain pathologies of the ocular surface in canine species.
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- 2020
24. Defining intraspecific conservation units in the endemic Cuban Rock Iguanas (Cyclura nubila nubila)
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L. Grisell Diaz-Ramirez, Vicente Berovides-Álvarez, Susette Castañeda-Rico, Kyle J. Shaney, Ella Vázquez-Domínguez, and Sayra Espindola
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0106 biological sciences ,0301 basic medicine ,Conservation of Natural Resources ,Evolution ,Science ,Population genetics ,Subspecies ,Biology ,010603 evolutionary biology ,01 natural sciences ,Article ,Evolutionary genetics ,03 medical and health sciences ,Genetic drift ,Effective population size ,Animals ,Ecosystem ,Genetic diversity ,Multidisciplinary ,Ecology ,Conservation biology ,Cuba ,Cyclura nubila ,biology.organism_classification ,Phylogeography ,030104 developmental biology ,Genetics, Population ,Iguanas ,Medicine ,Cyclura ,Microsatellite Repeats - Abstract
Defining conservation units is an important step in species management and requires interpretation of the genetic diversity and ecological function of the taxon being considered. We used the endemic Cuban Rock Iguanas (Cyclura nubila nubila) as a model to highlight this challenge and examined patterns of its intraspecific genetic diversity across Cuba. We evaluated nuclear (microsatellite loci) and mitochondrial diversity across eight populations from the island and its off-shore cays, and applied the population genetics results for assignment of Management Unit (MU) status and Evolutionary Significant Units (ESUs) based on phylogeographic and time of divergence information. We identified at least six distinct Cuban Rock Iguana MUs, encompassing demographically isolated and genetically differentiated populations across Cuba, most with low effective population size, declining populations, and with high risk of inbreeding and genetic drift. Hence, each MU should be considered of urgent conservation priority. Given the key ecological seed dispersal role of C. n. nubila, the disappearance of any MU could trigger the loss of local ecological functional diversity and major negative impacts on their ecosystems. Two divergent ESUs were also identified, exhibiting an historical east–west geographic separation on Cuba. Based on a Caribbean phylogeographic assessment, our findings strengthen the conclusion that all geographically and evolutionarily differentiated Cyclura species and subspecies across the archipelago warrant ESU distinction.
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- 2020
25. Effect of multiple agricultural stressors on freshwater ecosystems: The role of community structure, trophic status, and biodiversity-functioning relationships on ecosystem responses
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Talles Bruno Oliveira dos Anjos, Andreu Rico, Susana Romo, Francesco Polazzo, Marco Vighi, and Alba Arenas-Sánchez
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Environmental Engineering ,Ecology ,Community structure ,Biodiversity ,Fresh Water ,Q Science (General) ,Biology ,Pollution ,Freshwater ecosystem ,Environmental Chemistry ,Ecosystem ,Species richness ,Ecosystem respiration ,Eutrophication ,Waste Management and Disposal ,Trophic level - Abstract
The toxicity and widespread use of agricultural pesticides threaten freshwater biodiversity, but their long-term effects under different nutrient concentrations are poorly understood. We evaluated the single or combined effects of two pesticides (chlorpyrifos and diuron) under different nutrient regimes (mesotrophic and eutrophic) on community structure and ecosystem functions in replicated pond mesocosms. The individual application of nutrients and pesticides affected community composition and species richness. Ecosystem functioning was generally less sensitive to chemical stress than community structure, while eutrophication fostered the dominance of species that are more resilient to pesticides. Stressor interactions were significant at different time points, with late stressor interactions affecting the recovery of community composition. We also found that the correlation between biodiversity and relevant ecosystem functions, such as primary productivity and total ecosystem respiration, can be shifted from positive to negative under particular stress conditions. Our study demonstrates that nutrients enrichment is a key factor influencing the resilience of freshwater ecosystems to multiple stressors and that functional redundancy allows maintaining constant levels of functioning even under high toxic stress pressure.
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- 2022
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26. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
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Lara Gibellini, Sussan Nourshargh, Susanna Cardell, Wlodzimierz Maslinski, Mar Felipo-Benavent, Florian Mair, Hans-Martin Jäck, Lilly Lopez, Klaus Warnatz, John Trowsdale, Diana Ordonez, Marcus Eich, William Hwang, Anne Cooke, Dirk Mielenz, Alberto Orfao, Winfried F. Pickl, Vladimir Benes, Alice Yue, T. Vincent Shankey, Maria Tsoumakidou, Virginia Litwin, Gelo Victoriano Dela Cruz, Andrea Cavani, Sara De Biasi, Larissa Nogueira Almeida, Jonathan J M Landry, Claudia Haftmann, Charlotte Esser, Ana Cumano, Anneke Wilharm, Francesco Dieli, Rudi Beyaert, Alessio Mazzoni, Burkhard Ludewig, Carlo Pucillo, Dirk H. Busch, Joe Trotter, Stipan Jonjić, Marc Veldhoen, Josef Spidlen, Aja M. Rieger, Dieter Adam, Srijit Khan, Todd A. Fehniger, Giuseppe Matarese, Maximilien Evrard, Christian Maueröder, Steffen Schmitt, Kristin A. Hogquist, Barry Moran, Raghavendra Palankar, Markus Feuerer, S Schmid, Susann Rahmig, Amy E. Lovett-Racke, James V. Watson, Megan K. Levings, Susanne Melzer, Dinko Pavlinic, Christopher M. Harpur, Christina Stehle, A. Graham Pockley, Toshinori Nakayama, Attila Tárnok, Juhao Yang, Michael Lohoff, Paulo Vieira, Francisco Sala-de-Oyanguren, Christian Kurts, Anastasia Gangaev, Alfonso Blanco, Hans Scherer, Regine J. Dress, Bruno Silva-Santos, Kiyoshi Takeda, Bimba F. Hoyer, Ilenia Cammarata, Daryl Grummitt, Isabel Panse, Günnur Deniz, Bianka Baying, Friederike Ebner, Esther Schimisky, Leo Hansmann, Thomas Kamradt, Edwin van der Pol, Daniel Scott-Algara, Anna Iannone, Giorgia Alvisi, Sebastian R. Schulz, Francesco Liotta, Irmgard Förster, Beatriz Jávega, Hans-Peter Rahn, Caetano Reis e Sousa, Livius Penter, Xuetao Cao, David P. Sester, Keisuke Goda, Peter Wurst, Iain B. McInnes, Ricardo T. Gazzinelli, Federica Piancone, Gerald Willimsky, Yotam Raz, Pärt Peterson, Wolfgang Fritzsche, Yvonne Samstag, Martin Büscher, Thomas Schüler, Susanne Hartmann, Robert J. Wilkinson, Anna E. S. Brooks, Steven L. C. Ketelaars, Catherine Sautès-Fridman, Anna Rubartelli, Petra Bacher, Katja Kobow, Marco A. Cassatella, Andrea Hauser, Henrik E. Mei, Kilian Schober, Silvia Della Bella, Graham Anderson, Michael D. Ward, Garth Cameron, Sebastian Lunemann, Katharina Kriegsmann, Katarzyna M. Sitnik, Brice Gaudilliere, Chantip Dang-Heine, Marcello Pinti, Paul Klenerman, Frank A. Schildberg, Joana Barros-Martins, Laura G. Rico, Hanlin Zhang, Christian Münz, Thomas Dörner, Jakob Zimmermann, Andrea M. Cooper, Jonni S. Moore, Andreas Diefenbach, Yanling Liu, Wolfgang Bauer, Tobit Steinmetz, Katharina Pracht, Leonard Tan, Peter K. Jani, Alan M. Stall, Petra Hoffmann, Christine S. Falk, Jasmin Knopf, Simon Fillatreau, Hans-Dieter Volk, Luis E. Muñoz, David L. Haviland, William W. Agace, Jonathan Rebhahn, Ljiljana Cvetkovic, Mohamed Trebak, Jordi Petriz, Mario Clerici, Diether J. Recktenwald, Anders Ståhlberg, Tristan Holland, Helen M. McGuire, Sa A. Wang, Christian Kukat, Thomas Kroneis, Laura Cook, Wan Ting Kong, Xin M. Wang, Britta Engelhardt, Pierre Coulie, Genny Del Zotto, Sally A. Quataert, Kata Filkor, Gabriele Multhoff, Bartek Rajwa, Federica Calzetti, Hans Minderman, Cosima T. Baldari, Jens Geginat, Hervé Luche, Gert Van Isterdael, Linda Schadt, Sophia Urbanczyk, Giovanna Borsellino, Liping Yu, Dale I. Godfrey, Achille Anselmo, Rachael C. Walker, Andreas Grützkau, David W. Hedley, Birgit Sawitzki, Silvia Piconese, Maria Yazdanbakhsh, Burkhard Becher, Ramon Bellmas Sanz, Michael Delacher, Hyun-Dong Chang, Immanuel Andrä, Hans-Gustaf Ljunggren, José-Enrique O'Connor, Ahad Khalilnezhad, Sharon Sanderson, Federico Colombo, Götz R. A. Ehrhardt, Inga Sandrock, Enrico Lugli, Christian Bogdan, James B. Wing, Susann Müller, Tomohiro Kurosaki, Derek Davies, Ester B. M. Remmerswaal, Kylie M. Quinn, Christopher A. Hunter, Andreas Radbruch, Timothy P. Bushnell, Anna Erdei, Sabine Adam-Klages, Pascale Eede, Van Duc Dang, Rieke Winkelmann, Thomas Korn, Gemma A. Foulds, Dirk Baumjohann, Matthias Schiemann, Manfred Kopf, Jan Kisielow, Lisa Richter, Jochen Huehn, Gloria Martrus, Alexander Scheffold, Jessica G. Borger, Sidonia B G Eckle, John Bellamy Foster, Anna Katharina Simon, Alicia Wong, Mübeccel Akdis, Gisa Tiegs, Toralf Kaiser, James McCluskey, Anna Vittoria Mattioli, Aaron J. Marshall, Hui-Fern Koay, Eva Orlowski-Oliver, Anja E. Hauser, J. Paul Robinson, Jay K. Kolls, Luca Battistini, Mairi McGrath, Jane L. Grogan, Natalio Garbi, Timothy Tree, Kingston H. G. Mills, Stefan H. E. Kaufmann, Wolfgang Schuh, Ryan R. Brinkman, Tim R. Mosmann, Vincenzo Barnaba, Andreas Dolf, Lorenzo Cosmi, Bo Huang, Andreia C. Lino, Baerbel Keller, René A. W. van Lier, Alexandra J. Corbett, Paul S. Frenette, Pleun Hombrink, Helena Radbruch, Sofie Van Gassen, Olivier Lantz, Lorenzo Moretta, Désirée Kunkel, Kirsten A. Ward-Hartstonge, Armin Saalmüller, Leslie Y. T. Leung, Salvador Vento-Asturias, Paola Lanuti, Alicia Martínez-Romero, Sarah Warth, Zhiyong Poon, Diana Dudziak, Andrea Cossarizza, Kovit Pattanapanyasat, Konrad von Volkmann, Jessica P. Houston, Agnès Lehuen, Andrew Filby, Pratip K. Chattopadhyay, Stefano Casola, Annika Wiedemann, Hannes Stockinger, Jürgen Ruland, Arturo Zychlinsky, Claudia Waskow, Katrin Neumann, Ari Waisman, Lucienne Chatenoud, Sudipto Bari, Kamran Ghoreschi, David W. Galbraith, Yvan Saeys, Hamida Hammad, Andrea Gori, Miguel López-Botet, Gabriel Núñez, Sabine Ivison, Michael Hundemer, Dorothea Reimer, Mark C. Dessing, Günter J. Hämmerling, Rudolf A. Manz, Tomas Kalina, Jonas Hahn, Holden T. Maecker, Hendy Kristyanto, Martin S. Davey, Henning Ulrich, Michael L. Dustin, Takashi Saito, Yousuke Takahama, Milena Nasi, Johanna Huber, Jürgen Wienands, Paolo Dellabona, Andreas Schlitzer, Michael D. Leipold, Kerstin H. Mair, Christian Peth, Immo Prinz, Chiara Romagnani, José M. González-Navajas, Josephine Schlosser, Marina Saresella, Matthias Edinger, Dirk Brenner, Nicole Baumgarth, Rikard Holmdahl, Fang-Ping Huang, Guadalupe Herrera, Malte Paulsen, Gergely Toldi, Luka Cicin-Sain, Reiner Schulte, Christina E. Zielinski, Thomas Winkler, Christoph Goettlinger, Philip E. Boulais, Jennie H M Yang, Antonio Celada, Heike Kunze-Schumacher, Julia Tornack, Florian Ingelfinger, Jenny Mjösberg, Andy Riddell, Leonie Wegener, Thomas Höfer, Christoph Hess, James P. Di Santo, Anna E. Oja, J. Kühne, Willem van de Veen, Mary Bebawy, Alberto Mantovani, Bart Everts, Giovanna Lombardi, Laura Maggi, Anouk von Borstel, Pia Kvistborg, Elisabetta Traggiai, A Ochel, Nima Aghaeepour, Charles-Antoine Dutertre, Matthieu Allez, Thomas Höllt, Wenjun Ouyang, Regina Stark, Maries van den Broek, Shimon Sakaguchi, Paul K. Wallace, Silvano Sozzani, Francesca LaRosa, Annette Oxenius, Malgorzata J. Podolska, Ivana Marventano, Wilhelm Gerner, Oliver F. Wirz, Britta Frehse, Gevitha Ravichandran, Martin Herrmann, Carl S. Goodyear, Gary Warnes, Helen Ferry, Stefan Frischbutter, Tim R. Radstake, Salomé LeibundGut-Landmann, Yi Zhao, Axel Schulz, Angela Santoni, Pablo Engel, Daniela C. Hernández, Andreas Acs, Cristiano Scottà, Francesco Annunziato, Thomas Weisenburger, Wolfgang Beisker, Sue Chow, Fritz Melchers, Daniel E. Speiser, Immanuel Kwok, Florent Ginhoux, Dominic A. Boardman, Natalie Stanley, Carsten Watzl, Marie Follo, Erik Lubberts, Andreas Krueger, Susanne Ziegler, Göran K. Hansson, David Voehringer, Antonia Niedobitek, Eleni Christakou, Lai Guan Ng, Sabine Baumgart, Nicholas A Gherardin, Antonio Cosma, Orla Maguire, Jolene Bradford, Daniel Schraivogel, Linda Quatrini, Stephen D. Miller, Rheumatology, Università degli Studi di Modena e Reggio Emilia (UNIMORE), Deutsches Rheuma-ForschungsZentrum (DRFZ), Deutsches Rheuma-ForschungsZentrum, Swiss Institute of Allergy and Asthma Research (SIAF), Universität Zürich [Zürich] = University of Zurich (UZH), Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine, ex- Institut Universitaire Hématologie-IUH) (IRSL), Université de Paris (UP), Ecotaxie, microenvironnement et développement lymphocytaire (EMily (UMR_S_1160 / U1160)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Department of Internal Medicine, Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI)-DENOTHE Center, Institute of Clinical Molecular Biology, Kiel University, Department of Life Sciences [Siena, Italy], Università degli Studi di Siena = University of Siena (UNISI), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP), Dulbecco Telethon Institute/Department of Biology, Caprotec Bioanalytics GmbH, International Occultation Timing Association European Section (IOTA ES), International Occultation Timing Association European Section, European Molecular Biology Laboratory [Heidelberg] (EMBL), VIB-UGent Center for Inflammation Research [Gand, Belgique] (IRC), VIB [Belgium], Fondazione Santa Lucia (IRCCS), Department of Immunology, Chinese Academy of Medical Sciences, FIRC Institute of Molecular Oncology Foundation, IFOM, Istituto FIRC di Oncologia Molecolare (IFOM), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Physiopatology and Transplantation, University of Milan (DEPT), University of Milan, Monash University [Clayton], Institut des Maladies Emergentes et des Thérapies Innovantes (IMETI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institute of Cellular Pathology, Université Catholique de Louvain = Catholic University of Louvain (UCL), Lymphopoïèse (Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Experimental Immunology Unit, Dept. of Oncology, DIBIT San Raffaele Scientific Institute, Immunité Innée - Innate Immunity, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Department of Biopharmacy [Bruxelles, Belgium] (Institute for Medical Immunology IMI), Université libre de Bruxelles (ULB), Charité Hospital, Humboldt-Universität zu Berlin, Agency for science, technology and research [Singapore] (A*STAR), Laboratory of Molecular Immunology and the Howard Hughes Institute, Rockefeller University [New York], Kennedy Institute of Rheumatology [Oxford, UK], Imperial College London, Theodor Kocher Institute, University of Bern, Leibniz Research Institute for Environmental Medicine [Düsseldorf, Germany] ( IUF), Université Lumière - Lyon 2 (UL2), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), University of Edinburgh, Integrative Biology Program [Milano], Istituto Nazionale Genetica Molecolare [Milano] (INGM), Singapore Immunology Network (SIgN), Biomedical Sciences Institute (BMSI), Universitat de Barcelona (UB), Rheumatologie, Cell Biology, Department of medicine [Stockholm], Karolinska Institutet [Stockholm]-Karolinska University Hospital [Stockholm], Department for Internal Medicine 3, Institute for Clinical Immunology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Delft University of Technology (TU Delft), Medical Inflammation Research, Karolinska Institutet [Stockholm], Department of Photonics Engineering [Lyngby], Technical University of Denmark [Lyngby] (DTU), Dpt of Experimental Immunology [Braunschweig], Helmholtz Centre for Infection Research (HZI), Department of Internal Medicine V, Universität Heidelberg [Heidelberg], Department of Histology and Embryology, University of Rijeka, Freiburg University Medical Center, Nuffield Dept of Clinical Medicine, University of Oxford [Oxford]-NIHR Biomedical Research Centre, Institute of Integrative Biology, Molecular Biomedicine, Berlin Institute of Health (BIH), Laboratory for Lymphocyte Differentiation, RIKEN Research Center, Institutes of Molecular Medicine and Experimental Immunology, University of Bonn, Immunité et cancer (U932), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Department of Surgery [Vancouver, BC, Canada] (Child and Family Research Institute), University of British Columbia (UBC)-Child and Family Research Institute [Vancouver, BC, Canada], College of Food Science and Technology [Shangai], Shanghai Ocean University, Institute for Medical Microbiology and Hygiene, University of Marburg, King‘s College London, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Centre d'Immunophénomique (CIPHE), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Brustzentrum Kantonsspital St. Gallen, Immunotechnology Section, Vaccine Research Center, National Institutes of Health [Bethesda] (NIH)-National Institute of Allergy and Infectious Diseases, Heinrich Pette Institute [Hamburg], Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Department of Immunology and Cell Biology, Mario Negri Institute, Laboratory of Molecular Medicine and Biotechnology, Don C. Gnocchi ONLUS Foundation, Institute of Translational Medicine, Klinik für Dermatologie, Venerologie und Allergologie, School of Biochemistry and Immunology, Department of Medicine Huddinge, Karolinska Institutet [Stockholm]-Karolinska University Hospital [Stockholm]-Lipid Laboratory, Università di Genova, Dipartimento di Medicina Sperimentale, Department of Environmental Microbiology, Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research (UFZ), Department of Radiation Oncology [Munich], Ludwig-Maximilians-Universität München (LMU), Centre de Recherche Publique- Santé, Université du Luxembourg (Uni.lu), William Harvey Research Institute, Barts and the London Medical School, University of Michigan [Ann Arbor], University of Michigan System, Centro de Investigacion del Cancer (CSIC), Universitario de Salamanca, Molecular Pathology [Tartu, Estonia], University of Tartu, Hannover Medical School [Hannover] (MHH), Centre d'Immunologie de Marseille - Luminy (CIML), Monash Biomedicine Discovery Institute, Cytometry Laboratories and School of Veterinary Medicine, Purdue University [West Lafayette], Data Mining and Modelling for Biomedicine [Ghent, Belgium], VIB Center for Inflammation Research [Ghent, Belgium], Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, RIKEN Research Center for Allergy and Immunology, Osaka University [Osaka], Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), Institute of Medical Immunology [Berlin, Germany], FACS and Array Core Facility, Johannes Gutenberg - Universität Mainz (JGU), Otto-von-Guericke University [Magdeburg] (OVGU), SUPA School of Physics and Astronomy [University of St Andrews], University of St Andrews [Scotland]-Scottish Universities Physics Alliance (SUPA), Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), General Pathology and Immunology (GPI), University of Brescia, Université de Lausanne (UNIL), Terry Fox Laboratory, BC Cancer Agency (BCCRC)-British Columbia Cancer Agency Research Centre, Department of Molecular Immunology, Medizinische Universität Wien = Medical University of Vienna, Dept. Pediatric Cardiology, Universität Leipzig [Leipzig], Universitaetsklinikum Hamburg-Eppendorf = University Medical Center Hamburg-Eppendorf [Hamburg] (UKE), Center for Cardiovascular Sciences, Albany Medical College, Dept Pathol, Div Immunol, University of Cambridge [UK] (CAM), Department of Information Technology [Gent], Universiteit Gent, Department of Plant Systems Biology, Department of Plant Biotechnology and Genetics, Universiteit Gent = Ghent University [Belgium] (UGENT), Division of Molecular Immunology, Institute for Immunology, Department of Geological Sciences, University of Oregon [Eugene], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, University of Colorado [Colorado Springs] (UCCS), FACS laboratory, Cancer Research, London, Cancer Research UK, Regeneration in Hematopoiesis and Animal Models of Hematopoiesis, Faculty of Medicine, Dresden University of Technology, Barbara Davis Center for Childhood Diabetes (BDC), University of Colorado Anschutz [Aurora], School of Computer and Electronic Information [Guangxi University], Guangxi University [Nanning], School of Materials Science and Engineering, Nanyang Technological University [Singapour], Max Planck Institute for Infection Biology (MPIIB), Max-Planck-Gesellschaft, Work in the laboratory of Dieter Adam is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Projektnummer 125440785 – SFB 877, Project B2.Petra Hoffmann, Andrea Hauser, and Matthias Edinger thank BD Biosciences®, San José, CA, USA, and SKAN AG, Bale, Switzerland for fruitful cooperation during the development, construction, and installation of the GMP‐compliant cell sorting equipment and the Bavarian Immune Therapy Network (BayImmuNet) for financial support.Edwin van der Pol and Paola Lanuti acknowledge Aleksandra Gąsecka M.D. for excellent experimental support and Dr. Rienk Nieuwland for textual suggestions. This work was supported by the Netherlands Organisation for Scientific Research – Domain Applied and Engineering Sciences (NWO‐TTW), research program VENI 15924.Jessica G Borger, Kylie M Quinn, Mairi McGrath, and Regina Stark thank Francesco Siracusa and Patrick Maschmeyer for providing data.Larissa Nogueira Almeida was supported by DFG research grant MA 2273/14‐1. Rudolf A. Manz was supported by the Excellence Cluster 'Inflammation at Interfaces' (EXC 306/2).Susanne Hartmann and Friederike Ebner were supported by the German Research Foundation (GRK 2046).Hans Minderman was supported by NIH R50CA211108.This work was funded by the Deutsche Forschungsgemeinschaft through the grant TRR130 (project P11 and C03) to Thomas H. Winkler.Ramon Bellmàs Sanz, Jenny Kühne, and Christine S. Falk thank Jana Keil and Kerstin Daemen for excellent technical support. The work was funded by the Germany Research Foundation CRC738/B3 (CSF).The work by the Mei laboratory was supported by German Research Foundation Grant ME 3644/5‐1 and TRR130 TP24, the German Rheumatism Research Centre Berlin, European Union Innovative Medicines Initiative ‐ Joint Undertaking ‐ RTCure Grant Agreement 777357, the Else Kröner‐Fresenius‐Foundation, German Federal Ministry of Education and Research e:Med sysINFLAME Program Grant 01ZX1306B and KMU‐innovativ 'InnoCyt', and the Leibniz Science Campus for Chronic Inflammation (http://www.chronische-entzuendung.org).Axel Ronald Schulz, Antonio Cosma, Sabine Baumgart, Brice Gaudilliere, Helen M. McGuire, and Henrik E. Mei thank Michael D. Leipold for critically reading the manuscript.Christian Kukat acknowledges support from the ISAC SRL Emerging Leaders program.John Trowsdale received funding from the European Research Council under the European Union's Horizon 2020 research and innovation program (Grant Agreement 695551)., European Project: 7728036(1978), Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE), Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Università degli Studi di Firenze = University of Florence (UniFI)-DENOTHE Center, Università degli Studi di Milano = University of Milan (UNIMI), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Humboldt University Of Berlin, Leibniz Research Institute for Environmental Medicine [Düsseldorf, Germany] (IUF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Universität Heidelberg [Heidelberg] = Heidelberg University, Universitäts Klinikum Freiburg = University Medical Center Freiburg (Uniklinik), University of Oxford-NIHR Biomedical Research Centre, Universität Bonn = University of Bonn, Università degli Studi di Firenze = University of Florence (UniFI), Università degli studi di Genova = University of Genoa (UniGe), Universidad de Salamanca, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Otto-von-Guericke-Universität Magdeburg = Otto-von-Guericke University [Magdeburg] (OVGU), Université de Lausanne = University of Lausanne (UNIL), Universität Leipzig, Universiteit Gent = Ghent University (UGENT), HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany., Cossarizza, A., Chang, H. -D., Radbruch, A., Acs, A., Adam, D., Adam-Klages, S., Agace, W. W., Aghaeepour, N., Akdis, M., Allez, M., Almeida, L. N., Alvisi, G., Anderson, G., Andra, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, C. T., Bari, S., Barnaba, V., Barros-Martins, J., Battistini, L., Bauer, W., Baumgart, S., Baumgarth, N., Baumjohann, D., Baying, B., Bebawy, M., Becher, B., Beisker, W., Benes, V., Beyaert, R., Blanco, A., Boardman, D. A., Bogdan, C., Borger, J. G., Borsellino, G., Boulais, P. E., Bradford, J. A., Brenner, D., Brinkman, R. R., Brooks, A. E. S., Busch, D. H., Buscher, M., Bushnell, T. P., Calzetti, F., Cameron, G., Cammarata, I., Cao, X., Cardell, S. L., Casola, S., Cassatella, M. A., Cavani, A., Celada, A., Chatenoud, L., Chattopadhyay, P. K., Chow, S., Christakou, E., Cicin-Sain, L., Clerici, M., Colombo, F. S., Cook, L., Cooke, A., Cooper, A. M., Corbett, A. J., Cosma, A., Cosmi, L., Coulie, P. G., Cumano, A., Cvetkovic, L., Dang, V. D., Dang-Heine, C., Davey, M. S., Davies, D., De Biasi, S., Del Zotto, G., Dela Cruz, G. V., Delacher, M., Della Bella, S., Dellabona, P., Deniz, G., Dessing, M., Di Santo, J. P., Diefenbach, A., Dieli, F., Dolf, A., Dorner, T., Dress, R. J., Dudziak, D., Dustin, M., Dutertre, C. -A., Ebner, F., Eckle, S. B. G., Edinger, M., Eede, P., Ehrhardt, G. R. A., Eich, M., Engel, P., Engelhardt, B., Erdei, A., Esser, C., Everts, B., Evrard, M., Falk, C. S., Fehniger, T. A., Felipo-Benavent, M., Ferry, H., Feuerer, M., Filby, A., Filkor, K., Fillatreau, S., Follo, M., Forster, I., Foster, J., Foulds, G. A., Frehse, B., Frenette, P. S., Frischbutter, S., Fritzsche, W., Galbraith, D. W., Gangaev, A., Garbi, N., Gaudilliere, B., Gazzinelli, R. T., Geginat, J., Gerner, W., Gherardin, N. A., Ghoreschi, K., Gibellini, L., Ginhoux, F., Goda, K., Godfrey, D. I., Goettlinger, C., Gonzalez-Navajas, J. M., Goodyear, C. S., Gori, A., Grogan, J. L., Grummitt, D., Grutzkau, A., Haftmann, C., Hahn, J., Hammad, H., Hammerling, G., Hansmann, L., Hansson, G., Harpur, C. M., Hartmann, S., Hauser, A., Hauser, A. E., Haviland, D. L., Hedley, D., Hernandez, D. C., Herrera, G., Herrmann, M., Hess, C., Hofer, T., Hoffmann, P., Hogquist, K., Holland, T., Hollt, T., Holmdahl, R., Hombrink, P., Houston, J. P., Hoyer, B. F., Huang, B., Huang, F. -P., Huber, J. E., Huehn, J., Hundemer, M., Hunter, C. A., Hwang, W. Y. K., Iannone, A., Ingelfinger, F., Ivison, S. M., Jack, H. -M., Jani, P. K., Javega, B., Jonjic, S., Kaiser, T., Kalina, T., Kamradt, T., Kaufmann, S. H. E., Keller, B., Ketelaars, S. L. C., Khalilnezhad, A., Khan, S., Kisielow, J., Klenerman, P., Knopf, J., Koay, H. -F., Kobow, K., Kolls, J. K., Kong, W. T., Kopf, M., Korn, T., Kriegsmann, K., Kristyanto, H., Kroneis, T., Krueger, A., Kuhne, J., Kukat, C., Kunkel, D., Kunze-Schumacher, H., Kurosaki, T., Kurts, C., Kvistborg, P., Kwok, I., Landry, J., Lantz, O., Lanuti, P., Larosa, F., Lehuen, A., LeibundGut-Landmann, S., Leipold, M. D., Leung, L. Y. T., Levings, M. K., Lino, A. C., Liotta, F., Litwin, V., Liu, Y., Ljunggren, H. -G., Lohoff, M., Lombardi, G., Lopez, L., Lopez-Botet, M., Lovett-Racke, A. E., Lubberts, E., Luche, H., Ludewig, B., Lugli, E., Lunemann, S., Maecker, H. T., Maggi, L., Maguire, O., Mair, F., Mair, K. H., Mantovani, A., Manz, R. A., Marshall, A. J., Martinez-Romero, A., Martrus, G., Marventano, I., Maslinski, W., Matarese, G., Mattioli, A. V., Maueroder, C., Mazzoni, A., Mccluskey, J., Mcgrath, M., Mcguire, H. M., Mcinnes, I. B., Mei, H. E., Melchers, F., Melzer, S., Mielenz, D., Miller, S. D., Mills, K. H. G., Minderman, H., Mjosberg, J., Moore, J., Moran, B., Moretta, L., Mosmann, T. R., Muller, S., Multhoff, G., Munoz, L. E., Munz, C., Nakayama, T., Nasi, M., Neumann, K., Ng, L. G., Niedobitek, A., Nourshargh, S., Nunez, G., O'Connor, J. -E., Ochel, A., Oja, A., Ordonez, D., Orfao, A., Orlowski-Oliver, E., Ouyang, W., Oxenius, A., Palankar, R., Panse, I., Pattanapanyasat, K., Paulsen, M., Pavlinic, D., Penter, L., Peterson, P., Peth, C., Petriz, J., Piancone, F., Pickl, W. F., Piconese, S., Pinti, M., Pockley, A. G., Podolska, M. J., Poon, Z., Pracht, K., Prinz, I., Pucillo, C. E. M., Quataert, S. A., Quatrini, L., Quinn, K. M., Radbruch, H., Radstake, T. R. D. J., Rahmig, S., Rahn, H. -P., Rajwa, B., Ravichandran, G., Raz, Y., Rebhahn, J. A., Recktenwald, D., Reimer, D., Reis e Sousa, C., Remmerswaal, E. B. M., Richter, L., Rico, L. G., Riddell, A., Rieger, A. M., Robinson, J. P., Romagnani, C., Rubartelli, A., Ruland, J., Saalmuller, A., Saeys, Y., Saito, T., Sakaguchi, S., Sala-de-Oyanguren, F., Samstag, Y., Sanderson, S., Sandrock, I., Santoni, A., Sanz, R. B., Saresella, M., Sautes-Fridman, C., Sawitzki, B., Schadt, L., Scheffold, A., Scherer, H. U., Schiemann, M., Schildberg, F. A., Schimisky, E., Schlitzer, A., Schlosser, J., Schmid, S., Schmitt, S., Schober, K., Schraivogel, D., Schuh, W., Schuler, T., Schulte, R., Schulz, A. R., Schulz, S. R., Scotta, C., Scott-Algara, D., Sester, D. P., Shankey, T. V., Silva-Santos, B., Simon, A. K., Sitnik, K. M., Sozzani, S., Speiser, D. E., Spidlen, J., Stahlberg, A., Stall, A. M., Stanley, N., Stark, R., Stehle, C., Steinmetz, T., Stockinger, H., Takahama, Y., Takeda, K., Tan, L., Tarnok, A., Tiegs, G., Toldi, G., Tornack, J., Traggiai, E., Trebak, M., Tree, T. I. M., Trotter, J., Trowsdale, J., Tsoumakidou, M., Ulrich, H., Urbanczyk, S., van de Veen, W., van den Broek, M., van der Pol, E., Van Gassen, S., Van Isterdael, G., van Lier, R. A. W., Veldhoen, M., Vento-Asturias, S., Vieira, P., Voehringer, D., Volk, H. -D., von Borstel, A., von Volkmann, K., Waisman, A., Walker, R. V., Wallace, P. K., Wang, S. A., Wang, X. M., Ward, M. D., Ward-Hartstonge, K. A., Warnatz, K., Warnes, G., Warth, S., Waskow, C., Watson, J. V., Watzl, C., Wegener, L., Weisenburger, T., Wiedemann, A., Wienands, J., Wilharm, A., Wilkinson, R. J., Willimsky, G., Wing, J. B., Winkelmann, R., Winkler, T. H., Wirz, O. F., Wong, A., Wurst, P., Yang, J. H. M., Yang, J., Yazdanbakhsh, M., Yu, L., Yue, A., Zhang, H., Zhao, Y., Ziegler, S. M., Zielinski, C., Zimmermann, J., Zychlinsky, A., UCL - SSS/DDUV - Institut de Duve, UCL - SSS/DDUV/GECE - Génétique cellulaire, Netherlands Organization for Scientific Research, German Research Foundation, European Commission, European Research Council, Repositório da Universidade de Lisboa, CCA - Imaging and biomarkers, Experimental Immunology, AII - Infectious diseases, AII - Inflammatory diseases, Biomedical Engineering and Physics, ACS - Atherosclerosis & ischemic syndromes, and Landsteiner Laboratory
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0301 basic medicine ,Consensus ,Immunology ,Consensu ,Cell Separation ,Biology ,Article ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Guidelines ,Allergy and Immunology ,medicine ,Cell separation ,Immunology and Allergy ,Humans ,guidelines ,flow cytometry ,immunology ,medicine.diagnostic_test ,BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences ,Cell sorting ,Flow Cytometry ,Cell selection ,Data science ,3. Good health ,030104 developmental biology ,Phenotype ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti ,030215 immunology ,Human - Abstract
All authors: Andrea Cossarizza Hyun‐Dong Chang Andreas Radbruch Andreas Acs Dieter Adam Sabine Adam‐Klages William W. Agace Nima Aghaeepour Mübeccel Akdis Matthieu Allez Larissa Nogueira Almeida Giorgia Alvisi Graham Anderson Immanuel Andrä Francesco Annunziato Achille Anselmo Petra Bacher Cosima T. Baldari Sudipto Bari Vincenzo Barnaba Joana Barros‐Martins Luca Battistini Wolfgang Bauer Sabine Baumgart Nicole Baumgarth Dirk Baumjohann Bianka Baying Mary Bebawy Burkhard Becher Wolfgang Beisker Vladimir Benes Rudi Beyaert Alfonso Blanco Dominic A. Boardman Christian Bogdan Jessica G. Borger Giovanna Borsellino Philip E. Boulais Jolene A. Bradford Dirk Brenner Ryan R. Brinkman Anna E. S. Brooks Dirk H. Busch Martin Büscher Timothy P. Bushnell Federica Calzetti Garth Cameron Ilenia Cammarata Xuetao Cao Susanna L. Cardell Stefano Casola Marco A. Cassatella Andrea Cavani Antonio Celada Lucienne Chatenoud Pratip K. Chattopadhyay Sue Chow Eleni Christakou Luka Čičin‐Šain Mario Clerici Federico S. Colombo Laura Cook Anne Cooke Andrea M. Cooper Alexandra J. Corbett Antonio Cosma Lorenzo Cosmi Pierre G. Coulie Ana Cumano Ljiljana Cvetkovic Van Duc Dang Chantip Dang‐Heine Martin S. Davey Derek Davies Sara De Biasi Genny Del Zotto Gelo Victoriano Dela Cruz Michael Delacher Silvia Della Bella Paolo Dellabona Günnur Deniz Mark Dessing James P. Di Santo Andreas Diefenbach Francesco Dieli Andreas Dolf Thomas Dörner Regine J. Dress Diana Dudziak Michael Dustin Charles‐Antoine Dutertre Friederike Ebner Sidonia B. G. Eckle Matthias Edinger Pascale Eede Götz R.A. Ehrhardt Marcus Eich Pablo Engel Britta Engelhardt Anna Erdei Charlotte Esser Bart Everts Maximilien Evrard Christine S. Falk Todd A. Fehniger Mar Felipo‐Benavent Helen Ferry Markus Feuerer Andrew Filby Kata Filkor Simon Fillatreau Marie Follo Irmgard Förster John Foster Gemma A. Foulds Britta Frehse Paul S. Frenette Stefan Frischbutter Wolfgang Fritzsche David W. Galbraith Anastasia Gangaev Natalio Garbi Brice Gaudilliere Ricardo T. Gazzinelli Jens Geginat Wilhelm Gerner Nicholas A. Gherardin Kamran Ghoreschi Lara Gibellini Florent Ginhoux Keisuke Goda Dale I. Godfrey Christoph Goettlinger Jose M. González‐Navajas Carl S. Goodyear Andrea Gori Jane L. Grogan Daryl Grummitt Andreas Grützkau Claudia Haftmann Jonas Hahn Hamida Hammad Günter Hämmerling Leo Hansmann Goran Hansson Christopher M. Harpur Susanne Hartmann Andrea Hauser Anja E. Hauser David L. Haviland David Hedley Daniela C. Hernández Guadalupe Herrera Martin Herrmann Christoph Hess Thomas Höfer Petra Hoffmann Kristin Hogquist Tristan Holland Thomas Höllt Rikard Holmdahl Pleun Hombrink Jessica P. Houston Bimba F. Hoyer Bo Huang Fang‐Ping Huang Johanna E. Huber Jochen Huehn Michael Hundemer Christopher A. Hunter William Y. K. Hwang Anna Iannone Florian Ingelfinger Sabine M Ivison Hans‐Martin Jäck Peter K. Jani Beatriz Jávega Stipan Jonjic Toralf Kaiser Tomas Kalina Thomas Kamradt Stefan H. E. Kaufmann Baerbel Keller Steven L. C. Ketelaars Ahad Khalilnezhad Srijit Khan Jan Kisielow Paul Klenerman Jasmin Knopf Hui‐Fern Koay Katja Kobow Jay K. Kolls Wan Ting Kong Manfred Kopf Thomas Korn Katharina Kriegsmann Hendy Kristyanto Thomas Kroneis Andreas Krueger Jenny Kühne Christian Kukat Désirée Kunkel Heike Kunze‐Schumacher Tomohiro Kurosaki Christian Kurts Pia Kvistborg Immanuel Kwok Jonathan Landry Olivier Lantz Paola Lanuti Francesca LaRosa Agnès Lehuen Salomé LeibundGut‐Landmann Michael D. Leipold Leslie Y.T. Leung Megan K. Levings Andreia C. Lino Francesco Liotta Virginia Litwin Yanling Liu Hans‐Gustaf Ljunggren Michael Lohoff Giovanna Lombardi Lilly Lopez Miguel López‐Botet Amy E. Lovett‐Racke Erik Lubberts Herve Luche Burkhard Ludewig Enrico Lugli Sebastian Lunemann Holden T. Maecker Laura Maggi Orla Maguire Florian Mair Kerstin H. Mair Alberto Mantovani Rudolf A. Manz Aaron J. Marshall Alicia Martínez‐Romero Glòria Martrus Ivana Marventano Wlodzimierz Maslinski Giuseppe Matarese Anna Vittoria Mattioli Christian Maueröder Alessio Mazzoni James McCluskey Mairi McGrath Helen M. McGuire Iain B. McInnes Henrik E. Mei Fritz Melchers Susanne Melzer Dirk Mielenz Stephen D. Miller Kingston H.G. Mills Hans Minderman Jenny Mjösberg Jonni Moore Barry Moran Lorenzo Moretta Tim R. Mosmann Susann Müller Gabriele Multhoff Luis Enrique Muñoz Christian Münz Toshinori Nakayama Milena Nasi Katrin Neumann Lai Guan Ng Antonia Niedobitek Sussan Nourshargh Gabriel Núñez José‐Enrique O'Connor Aaron Ochel Anna Oja Diana Ordonez Alberto Orfao Eva Orlowski‐Oliver Wenjun Ouyang Annette Oxenius Raghavendra Palankar Isabel Panse Kovit Pattanapanyasat Malte Paulsen Dinko Pavlinic Livius Penter Pärt Peterson Christian Peth Jordi Petriz Federica Piancone Winfried F. Pickl Silvia Piconese Marcello Pinti A. Graham Pockley Malgorzata Justyna Podolska Zhiyong Poon Katharina Pracht Immo Prinz Carlo E. M. Pucillo Sally A. Quataert Linda Quatrini Kylie M. Quinn Helena Radbruch Tim R. D. J. Radstake Susann Rahmig Hans‐Peter Rahn Bartek Rajwa Gevitha Ravichandran Yotam Raz Jonathan A. Rebhahn Diether Recktenwald Dorothea Reimer Caetano Reis e Sousa Ester B.M. Remmerswaal Lisa Richter Laura G. Rico Andy Riddell Aja M. Rieger J. Paul Robinson Chiara Romagnani Anna Rubartelli Jürgen Ruland Armin Saalmüller Yvan Saeys Takashi Saito Shimon Sakaguchi Francisco Sala‐de‐Oyanguren Yvonne Samstag Sharon Sanderson Inga Sandrock Angela Santoni Ramon Bellmàs Sanz Marina Saresella Catherine Sautes‐Fridman Birgit Sawitzki Linda Schadt Alexander Scheffold Hans U. Scherer Matthias Schiemann Frank A. Schildberg Esther Schimisky Andreas Schlitzer Josephine Schlosser Stephan Schmid Steffen Schmitt Kilian Schober Daniel Schraivogel Wolfgang Schuh Thomas Schüler Reiner Schulte Axel Ronald Schulz Sebastian R. Schulz Cristiano Scottá Daniel Scott‐Algara David P. Sester T. Vincent Shankey Bruno Silva‐Santos Anna Katharina Simon Katarzyna M. Sitnik Silvano Sozzani Daniel E. Speiser Josef Spidlen Anders Stahlberg Alan M. Stall Natalie Stanley Regina Stark Christina Stehle Tobit Steinmetz Hannes Stockinger Yousuke Takahama Kiyoshi Takeda Leonard Tan Attila Tárnok Gisa Tiegs Gergely Toldi Julia Tornack Elisabetta Traggiai Mohamed Trebak Timothy I.M. Tree Joe Trotter John Trowsdale Maria Tsoumakidou Henning Ulrich Sophia Urbanczyk Willem van de Veen Maries van den Broek Edwin van der Pol Sofie Van Gassen Gert Van Isterdael René A.W. van Lier Marc Veldhoen Salvador Vento‐Asturias Paulo Vieira David Voehringer Hans‐Dieter Volk Anouk von Borstel Konrad von Volkmann Ari Waisman Rachael V. Walker Paul K. Wallace Sa A. Wang Xin M. Wang Michael D. Ward Kirsten A Ward‐Hartstonge Klaus Warnatz Gary Warnes Sarah Warth Claudia Waskow James V. Watson Carsten Watzl Leonie Wegener Thomas Weisenburger Annika Wiedemann Jürgen Wienands Anneke Wilharm Robert John Wilkinson Gerald Willimsky James B. Wing Rieke Winkelmann Thomas H. Winkler Oliver F. Wirz Alicia Wong Peter Wurst Jennie H. M. Yang Juhao Yang Maria Yazdanbakhsh Liping Yu Alice Yue Hanlin Zhang Yi Zhao Susanne Maria Ziegler Christina Zielinski Jakob Zimmermann Arturo Zychlinsky., These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion., This work was supported by the Netherlands Organisation for Scientific Research – Domain Applied and Engineering Sciences (NWO-TTW), research program VENI 15924. This work was funded by the Deutsche Forschungsgemeinschaft. European Union Innovative Medicines Initiative - Joint Undertaking - RTCure Grant Agreement 777357 and innovation program (Grant Agreement 695551).
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- 2019
27. Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome
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Georges Blattner, Fang Zhang, Winston Vetharoy, Marianna Romito, Adrian J. Thrasher, Elizabeth Rivers, Dariusz Ladon, Marta Zinicola, Diego Leon-Rico, Rajeev Rai, Giandomenico Turchiano, Giorgia Santilli, Geoffroy Andrieux, and Alessia Cavazza
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0301 basic medicine ,Male ,Myeloid ,Wiskott–Aldrich syndrome ,medicine.medical_treatment ,Genetic enhancement ,T-Lymphocytes ,General Physics and Astronomy ,02 engineering and technology ,Hematopoietic stem cell transplantation ,Stem-cell biotechnology ,Mice ,Genome editing ,hemic and lymphatic diseases ,Myeloid Cells ,lcsh:Science ,Gene Editing ,Multidisciplinary ,Molecular medicine ,Hematopoietic Stem Cell Transplantation ,High-Throughput Nucleotide Sequencing ,021001 nanoscience & nanotechnology ,3. Good health ,Wiskott-Aldrich Syndrome ,Haematopoiesis ,medicine.anatomical_structure ,Primary immunodeficiency disorders ,Female ,Stem cell ,0210 nano-technology ,Wiskott-Aldrich Syndrome Protein ,Blood Platelets ,Science ,macromolecular substances ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Article ,03 medical and health sciences ,medicine ,Animals ,Humans ,Cell Lineage ,Progenitor cell ,Codon ,Mutagenicity Tests ,Macrophages ,General Chemistry ,Genetic Therapy ,medicine.disease ,Hematopoietic Stem Cells ,Targeted gene repair ,030104 developmental biology ,HEK293 Cells ,Genetic Loci ,Genetic engineering ,Cancer research ,lcsh:Q ,CRISPR-Cas Systems - Abstract
Wiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency with severe platelet abnormalities and complex immunodeficiency. Although clinical gene therapy approaches using lentiviral vectors have produced encouraging results, full immune and platelet reconstitution is not always achieved. Here we show that a CRISPR/Cas9-based genome editing strategy allows the precise correction of WAS mutations in up to 60% of human hematopoietic stem and progenitor cells (HSPCs), without impairing cell viability and differentiation potential. Delivery of the editing reagents to WAS HSPCs led to full rescue of WASp expression and correction of functional defects in myeloid and lymphoid cells. Primary and secondary transplantation of corrected WAS HSPCs into immunodeficient mice showed persistence of edited cells for up to 26 weeks and efficient targeting of long-term repopulating stem cells. Finally, no major genotoxicity was associated with the gene editing process, paving the way for an alternative, yet highly efficient and safe therapy., In recent years, hematopoietic stem cells gene editing has emerged as a promising tool to treat blood disorders. Here the authors develop a CRISPR/Cas9-based genome editing strategy that allows the precise correction of Wiskott-Aldrich Syndrome in vitro and in vivo with high efficiency.
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- 2020
28. Ocorrência de ectoparasitas no roedor Oligoryzomys microtis em regiões ao norte do Departamento de La Paz, Bolívia
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A. Rico, J. Martinez, Susana G. Revollo-Cadima, Jorge Salazar-Bravo, I.V. Sanchez-Roman, M.S.C. Graça, P.A.S. Basto, E.I. Alandia, and M.I. Moya
- Subjects
Oligoryzomys ,General Veterinary ,biology ,040301 veterinary sciences ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,biology.organism_classification ,040201 dairy & animal science ,SF1-1100 ,roedores ,Animal culture ,0403 veterinary science ,Humanities ,ectoparasitas - Abstract
RESUMO O objetivo deste trabalho foi mapear e descrever a ocorrência de roedores e seus ectoparasitas nas regiões norte do departamento de La Paz, Bolívia. De abril a maio e julho a agosto de 2017, 80 indivíduos da espécie Oligoryzomys microtis foram capturados em armadilhas vivas. Uma amostra aleatória de 36 indivíduos foi analisada quanto à presença de ectoparasitas, encontrando um total de 458 ectoparasitas. Os roedores foram capturados em dois tipos de ambientes: floresta secundária e áreas cultivadas, sendo a área cultivada a área com maior captação de roedores. Dos ectoparasitas, Laelaps sp. foi o gênero com maior abundância e Mysolaelaps sp. o mais prevalente; o gênero Polygenis sp. é aparentemente descrito pela primeira vez na selva amazônica, exigindo estudos adicionais para entender melhor os patógenos que são transmitidos no parasitismo desta pulga em roedores devido à sua importância no ecossistema e para a saúde pública.
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- 2020
29. Comparison of genomic diversity and structure of sable antelope (Hippotragus niger) in zoos, conservation centers, and private ranches in North America
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Budhan S. Pukazhenthi, Gina M. Ferrie, Pavel Dobrynin, Holly J Haefele, Gaik Tamazian, Cody W. Edwards, David E. Wildt, Susette Castañeda-Rico, Katherine R. Murphy, Jesús E. Maldonado, Klaus-Peter Koepfli, and Rebecca M. Gooley
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0106 biological sciences ,0301 basic medicine ,ex situ management ,Hippotragus ,sable antelope ,Population ,lcsh:Evolution ,inbreeding ,Metapopulation ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,Genetic drift ,lcsh:QH359-425 ,Genetics ,education ,Sable antelope ,Ecology, Evolution, Behavior and Systematics ,Genetic diversity ,education.field_of_study ,biology ,Ecology ,metapopulation ,Original Articles ,genetic diversity ,biology.organism_classification ,030104 developmental biology ,Habitat ,Original Article ,genetic drift ,General Agricultural and Biological Sciences ,Inbreeding - Abstract
As we enter the sixth mass extinction, many species that are no longer self‐sustaining in their natural habitat will require ex situ management. Zoos have finite resources for ex situ management, and there is a need for holistic conservation programs between the public and private sector. Ex situ populations of sable antelope, Hippotragus niger, have existed in zoos and privately owned ranches in North America since the 1910s. Unknown founder representation and relatedness has made the genetic management of this species challenging within zoos, while populations on privately owned ranches are managed independently and retain minimal‐to‐no pedigree history. Consequences of such challenges include an increased risk of inbreeding and a loss of genetic diversity. Here, we developed and applied a customized targeted sequence capture panel based on 5,000 genomewide single‐nucleotide polymorphisms to investigate the genomic diversity present in these uniquely managed populations. We genotyped 111 sable antelope: 23 from zoos, 43 from a single conservation center, and 45 from ranches. We found significantly higher genetic diversity and significantly lower inbreeding in herds housed in zoos and conservation centers, when compared to those in privately owned ranches, likely due to genetic‐based breeding recommendations implemented in the former populations. Genetic clustering was strong among all three populations, possibly as a result of genetic drift. We propose that the North American ex situ population of sable antelope would benefit from a metapopulation management system, to halt genetic drift, reduce the occurrence of inbreeding, and enable sustainable population sizes to be managed ex situ.
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- 2020
30. Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter
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José Guillermo Rico-Ruiz, Pedro Martínez-Hernández, Luis Herrera-Estrella, José Fabricio López-Hernández, Lenin Yong-Villalobos, Alfredo Herrera-Estrella, Nohemí Carreras-Villaseñor, Damar Lizbeth López-Arredondo, and Ricardo A. Chávez Montes
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Phosphite ,Genes, Fungal ,lcsh:QR1-502 ,Bioengineering ,Applied Microbiology and Biotechnology ,lcsh:Microbiology ,03 medical and health sciences ,Bacterial Proteins ,Phosphite oxidoreductase (PTXD) ,Cloning, Molecular ,Promoter Regions, Genetic ,Gene ,Trichoderma reesei ,030304 developmental biology ,Trichoderma ,0303 health sciences ,Reporter gene ,biology ,030306 microbiology ,Growth marker ,Research ,Promoter ,biology.organism_classification ,Recombinant Proteins ,ccg6 promoter ,DNA binding site ,Biochemistry ,Heterologous expression ,Target protein ,Contamination control ,Oxidoreductases ,Biotechnology - Abstract
Background Trichoderma species are among the most effective cell factories to produce recombinant proteins, whose productivity relies on the molecular toolkit and promoters available for the expression of the target protein. Although inducible promoter systems have been developed for producing recombinant proteins in Trichoderma, constitutive promoters are often a desirable alternative. Constitutive promoters are simple to use, do not require external stimuli or chemical inducers to be activated, and lead to purer enzyme preparations. Moreover, most of the promoters for homologous and heterologous expression reported in Trichoderma have been commonly evaluated by directly assessing production of industrial enzymes, requiring optimization of laborious protocols. Results Here we report the identification of Pccg6, a novel Trichoderma atroviride constitutive promoter, that has similar transcriptional strength as that of the commonly used pki1 promoter. Pccg6 displayed conserved arrangements of transcription factor binding sites between promoter sequences of Trichoderma ccg6 orthologues genes, potentially involved in their regulatory properties. The predicted ccg6-encoded protein potentially belongs to the SPE1/SPI1 protein family and shares high identity with CCG6 orthologue sequences from other fungal species including Trichoderma reesei, Trichoderma virens, Trichoderma asperellum, and to a lesser extent to that of Neurospora crassa. We also report the use of the Pccg6 promoter to drive the expression of PTXD, a phosphite oxidoreductase of bacterial origin, which allowed T. atroviride to utilize phosphite as a sole source of phosphorus. We propose ptxD as a growth reporter gene that allows real-time comparison of the functionality of different promoters by monitoring growth of Trichoderma transgenic lines and enzymatic activity of PTXD. Finally, we show that constitutive expression of ptxD provided T. atroviride a competitive advantage to outgrow bacterial contaminants when supplied with phosphite as a sole source of phosphorus. Conclusions A new constitutive promoter, ccg6, for expression of homologous and heterologous proteins has been identified and tested in T. atroviride to express PTXD, which resulted in an effective and visible phenotype to evaluate transcriptional activity of sequence promoters. Use of PTXD as a growth marker holds great potential for assessing activity of other promoters and for biotechnological applications as a contamination control system.
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- 2020
31. Antidiabetes and Antioxidant agents from Clausena excavata root as medicinal plant of Myanmar
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Alfinda Novi Kristanti, Nanik Siti Aminah, Yoshiaki Takaya, Hnin Thanda Aung, a Tin Myo Thant, and Rico Ramadhan
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Antioxidant ,antioxidant ,DPPH ,medicine.medical_treatment ,Clausena excavata ,medicine.disease_cause ,dpph ,01 natural sciences ,antidiabetes ,chemistry.chemical_compound ,Materials Chemistry ,medicine ,IC50 ,QD1-999 ,Acarbose ,Traditional medicine ,biology ,010405 organic chemistry ,Chemistry ,dentatin ,General Chemistry ,heptaphylline ,biology.organism_classification ,Ascorbic acid ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,α-glucosidase ,Maltase ,clausena excavata ,Oxidative stress ,medicine.drug - Abstract
All around the world, patients with diabetes and the prevalence of its disease are currently growing. Due to these side effects of oral hypoglycemic agents and oxidative stress in complicating diabetes, there is growing interest in drugs, which possess dual function as both type II diabetes mellitus treatment and oxidative stress treatment. The objective of this research is to search effective antidiabetes and antioxidant bioactive compounds from the Myanmar medicinal plantClausena excavata. The root part ofC. excavatawas successfully extracted with 95% ethanol and followed by column chromatographic separation technique. The structure of isolated pure compounds was elucidated by using methods of spectroscopic such as UV-Vis, IR, NMR and HRFABMS spectrometry. The α-glucosidase inhibition assay was performed against baker’s yeast and rat intestine (sucrose and maltase) α-glucosidases. The activity of isolated compounds’ antioxidant was measured by using DPPH assay. Among the tested enzymes, the two isolated compounds, which were dentatin (1) and heptaphylline (2), exhibited highest inhibitory on maltase enzymes with IC50values 6.75 and 11.46 μM; as positive control, acarbose (IC50, 2.35 μM) was utilized. Moreover, scavenging activity was found to be present upon seeing the result of antioxidant activity investigation of (1) and (2) (IC50values 2.66 and 1.55 mM), where ascorbic acid (IC500.012 mM) was used as standard. Both compounds showed their antidiabetic and antioxidant activity with different fashion, especially exhibited strongest activity against on maltase α-glucosidase.
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- 2019
32. Hypogammaglobulinemia and Infections in Patients With Multiple Sclerosis Treated With Rituximab
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Marine Perriguey, Jan-Patrick Stellmann, Adil Maarouf, Pierre Durozard, Sarah Demortiere, Jean Pelletier, Audrey Rico, Clemence Boutiere, Bertrand Audoin, Pôle de Neurosciences Cliniques, Service de Neurologie, APHM, Hôpital de la Timone, Aix-Marseille University, Marseille, France, Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Exploration Métabolique par Résonance Magnétique [Hôpital de la Timone - APHM] (CEMEREM), Hôpital de la Timone [CHU - APHM] (TIMONE)-Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), and APHM, Hôpital de la Timone, Département de Neuroradiologie
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Adult ,Male ,medicine.medical_specialty ,Infections ,Gastroenterology ,Article ,Immunoglobulin G ,Hypogammaglobulinemia ,Multiple Sclerosis, Relapsing-Remitting ,Agammaglobulinemia ,Internal medicine ,medicine ,Humans ,Immunologic Factors ,In patient ,Prospective Studies ,Expanded Disability Status Scale ,biology ,Proportional hazards model ,business.industry ,Multiple sclerosis ,Hazard ratio ,[SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy ,Middle Aged ,medicine.disease ,Neurology ,biology.protein ,Female ,Rituximab ,Neurology (clinical) ,business ,medicine.drug - Abstract
Background and ObjectivesTo determine the frequency of hypogammaglobulinemia and infections in patients with multiple sclerosis (PwMS) receiving rituximab (RTX).MethodsThis prospective observational study included all consecutive PwMS receiving RTX at the university hospital of Marseille, France, between 2015 and 2020. Patient visits occurred at least every 6 months.ResultsWe included 188 patients (151 with relapsing-remitting MS; the mean age was 43.4 years [SD 12.9], median disease duration 10 years [range 0–36], median Expanded Disability Status Scale 5 [range 0–8], median follow-up 3.5 years [range 1–5.8], and median number of RTX infusions 5 [range 1–9]). Overall, 317 symptomatic infections and 13 severe infections occurred in 133 of 188 (70.7%) and 11 of 188 (5.9%) patients, respectively. After 4 years, 24.4% of patients (95% CI 18.0–33.1) were free of any infection and 92.0% (95% CI 87.1–97.1) had not experienced a severe infection. At RTX onset, the immunoglobulin G (IgG) level was abnormal in 32 of 188 (17%) patients. After RTX, IgG level was p = 0.03). The risk of reduced IgG level p = 0.01).DiscussionIn PwMS receiving RTX, reduced IgG level was frequent and interacted with the risk of infection.
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- 2021
33. Strongyloides stercoralis and cytomegalovirus coinfection in a patient with a transplanted kidney
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Mohammad Amin Fallahzadeh, Nina T. Rico, Huang He, Elham Vahhab, Dina Abdelwahab Elhamahmi, and Uriel Sandkovsky
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Case Studies ,biology ,business.industry ,Congenital cytomegalovirus infection ,medicine ,Transplanted kidney ,Coinfection ,General Medicine ,medicine.disease ,business ,biology.organism_classification ,Virology ,Strongyloides stercoralis - Abstract
Cytomegalovirus is a major opportunistic infection after transplantation with significant morbidity and mortality for solid organ transplant recipients. Unrecognized infection with Strongyloides stercoralis may result in significant morbidity and mortality in immunocompromised patients. Coinfection with multiple pathogens is possible, leading to diagnostic delays, and may make treatment more challenging. We report a case of coinfection with S. stercoralis and cytomegalovirus in a kidney transplant patient that resulted in pneumonitis, gastritis, and cholecystitis.
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- 2021
34. Untargeted Metabolome- and Transcriptome-Wide Association Study Suggests Causal Genes Modulating Metabolite Concentrations in Urine
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Anneke Brümmer, Reyhan Sönmez Flitman, Rico Rueedi, Sven Bergmann, Bita Khalili, and Zoltán Kutalik
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Magnetic Resonance Spectroscopy ,Metabolite ,Genome-wide association study ,Computational biology ,Biology ,Biochemistry ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Metabolomics ,Alms1 ,Hps1 ,N-acetylated compounds ,Nat8 ,Pyroxd2 ,genome-wide association study ,metabolomics ,transcriptomics ,trimethylamine ,ALMS1 ,HPS1 ,NAT8 ,PYROXD2 ,Gene expression ,Metabolome ,Humans ,Gene ,030304 developmental biology ,0303 health sciences ,Mendelian Randomization Analysis ,General Chemistry ,Body Fluids ,3. Good health ,chemistry ,030217 neurology & neurosurgery - Abstract
Gene products can affect the concentrations of small molecules (aka "metabolites"), and conversely, some metabolites can modulate the concentrations of gene transcripts. While many specific instances of this interplay have been revealed, a global approach to systematically uncover human gene-metabolite interactions is still lacking. We performed a metabolome- and transcriptome-wide association study to identify genes influencing the human metabolome using untargeted metabolome features, extracted from 1 H nuclear magnetic resonance spectroscopy (NMR) of urine samples, and gene expression levels, quantified from RNA-Seq of lymphoblastoid cell lines (LCL) from 555 healthy individuals. We identified 20 study-wide significant associations corresponding to 15 genes, of which 5 associations (with 2 genes) were confirmed with follow-up NMR data. Using metabomatching, we identified the metabolites corresponding to metabolome features associated with the genes, namely, N-acetylated compounds with ALMS1 and trimethylamine (TMA) with HPS1. Finally, Mendelian randomization analysis supported a potential causal link between the expression of genes in both the ALMS1- and HPS1-loci and their associated metabolite concentrations. In the case of HPS1, we additionally observed that TMA concentration likely exhibits a reverse causal effect on HPS1 expression levels, indicating a negative feedback loop. Our study highlights how the integration of metabolomics, gene expression, and genetic data can pinpoint causal genes modulating metabolite concentrations.
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- 2021
35. Molecular evidence and ecological niche modeling reveal an extensive hybrid zone among three Bursera species (section Bullockia)
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Antonio González Rodríguez, Yessica Rico, Andrés Lira Noriega, and Eduardo Quintero Melecio
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Atmospheric Science ,Leaves ,Heredity ,Chloroplasts ,Introgression ,Plant Science ,Geographical locations ,Macroecology ,Data Management ,Climatology ,Multidisciplinary ,biology ,Ecology ,Bursera ,Plant Anatomy ,Phylogenetic Analysis ,Phylogenetics ,Genetic Mapping ,Medicine ,Ecological Niches ,Research Article ,Computer and Information Sciences ,Evolutionary Processes ,Science ,Hybrid zone ,Genetics ,Evolutionary Systematics ,Endemism ,Paleoclimatology ,Hybridization ,Mexico ,Ecosystem ,Hybrid ,Taxonomy ,Ecological niche ,Evolutionary Biology ,Base Sequence ,Ecology and Environmental Sciences ,Biology and Life Sciences ,Paleontology ,biology.organism_classification ,Environmental niche modelling ,Haplotypes ,North America ,Earth Sciences ,Hybridization, Genetic ,People and places - Abstract
The genusBursera, includes ~100 shrub and trees species in tropical dry forests with its center of diversification and endemism in Mexico. Morphologically intermediate individuals have commonly been observed in MexicanBurserain areas where closely related species coexist. These individuals are assumed to result from interspecific hybridization, but no molecular evidence has supported their hybrid origins. This study aimed to investigate the existence of interspecific hybridization among three MexicanBurseraspecies (Bullockiasection:B.cuneata,B.palmeriandB.bipinnata) from nine populations based on DNA sequences (three nuclear and four chloroplast regions) and ecological niche modeling for three past and two future scenario projections. Results from the only two polymorphic nuclear regions (PEPC, ETS) supported the hybrid origin of morphologically intermediate individuals and revealed thatB.cuneataandB.bipinnataare the parental species that are genetically closer to the putative hybrids. Ecological niche modeling accurately predicted the occurrence of putative hybrid populations and showed a potential hybrid zone extending in a larger area (74,000 km2) than previously thought. Paleo-reconstructions showed a potential hybrid zone existing from the Last Glacial Maximum (~ 21 kya) that has increased since the late Holocene to the present. Future ecological niche projections show an increment of suitability of the potential hybrid zone for 2050 and 2070 relative to the present. Hybrid zone changes responded mostly to an increase in elevational ranges. Our study provides the first insight of an extensive hybrid zone among three MexicanBurseraspecies based on molecular data and ecological niche modeling.
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- 2021
36. Postprocedural Troponin Elevation and Mortality After Transcatheter Aortic Valve Implantation
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Erik W. Holy, Robert Manka, Barbara E. Stähli, Florin Stöckli, Rico Brütsch, Matthias Schindler, Jonathan M Michel, Christian Templin, Paul R. Vogt, Philipp Jakob, Frank Ruschitzka, Markus Kasel, University of Zurich, and Stähli, Barbara E
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medicine.medical_specialty ,Transcatheter aortic ,610 Medicine & health ,risk stratification ,2705 Cardiology and Cardiovascular Medicine ,Transcatheter Aortic Valve Replacement ,Risk Factors ,Catheter-Based Coronary and Valvular Interventions ,Internal medicine ,Medicine ,Humans ,Diseases of the circulatory (Cardiovascular) system ,Myocardial infarction ,Mortality prediction ,transcatheter aortic valve implantation ,Original Research ,biology ,business.industry ,aortic stenosis ,Aortic Valve Stenosis ,medicine.disease ,Troponin ,Interventional Cardiology ,Treatment Outcome ,myocardial infarction ,Aortic Valve ,RC666-701 ,Risk stratification ,10209 Clinic for Cardiology ,biology.protein ,Cardiology ,Mortality/Survival ,Cardiology and Cardiovascular Medicine ,business - Abstract
Background This study sought to investigate the role of postprocedural troponin elevations in mortality prediction after transcatheter aortic valve implantation and to define the threshold at which clinically relevant postprocedure myocardial injury determines mortality. Methods and Results A total of 1333 consecutive patients with transcatheter aortic valve implantation with available postprocedural high‐sensitivity cardiac troponin T measurements were included in the analysis. The threshold at which postprocedure myocardial injury determines long‐term mortality was identified using restricted cubic spline analysis. A >18.3‐fold increase of troponin above the upper reference limit was identified as threshold for relevant postprocedure myocardial injury. Associations remained significant in a landmark analysis between 30 days and 2 years (hazard ratio [HR], 1.61, [95% CI, 1.13–2.28]; P =0.01), after adjusting for known confounders (adjusted HR, 1.90 [95% CI, 1.40–2.57]; P P P P =0.003). Conclusions This study determined a troponin threshold for the identification of patients at increased mortality risk after transcatheter aortic valve implantation. The proposed definition of postprocedure myocardial injury advances risk stratification in patients with transcatheter aortic valve implantation and may assist in postprocedural patient management.
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- 2021
37. First report on the swim bladder index, proximate composition, and fatty acid analysis of swim bladder from cultured Totoaba macdonaldi fed compound aquafeeds
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Garrett M. Goto, Laura D. Gray, Adam M. Sachs, Miguel Castellanos-Rico, Mayra L. González-Félix, Steven D. Gaines, and Martin Perez-Velazquez
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chemistry.chemical_classification ,Fatty acid ,SH1-691 ,Aquatic Science ,Biology ,Proximate composition ,Soy protein concentrate ,Swim bladder quality ,chemistry ,Swim bladder ,Aquaculture. Fisheries. Angling ,Animal Science and Zoology ,Food science ,Aquaculture diets ,Totoaba - Abstract
Totoaba (Totoaba macdonaldi) is a large species of croaker, endemic to the upper Gulf of California in Mexico, which has been under extreme poaching pressure due to its highly valuable swim bladder, or buche. For more than 20 years, totoaba aquaculture has been developing as one, among many, conservation strategies in the ongoing management of the species. Competing with illegally poached buche requires farming high quality product. This study provides the first report of the influence of alternative aquaculture feeds – fishmeal versus soy protein concentrate (SPC) – on key cultured totoaba traits that affect the market value of its swim bladder. Since the price of farmed buche is key to the successful displacement of illegally poached product, options for replacing expensive fishmeal are key to conservation success. A variety of characteristics related to buche size and quality were affected by diet composition, but partial inclusion of soy protein as a substitute for fishmeal can produce buche of comparable quality to product grown from more expensive fishmeal diets. These findings highlight a path for diet changes to produce high value totoaba swim bladders from aquaculture at substantially lower costs.
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- 2021
38. Bovine apo-lactoferrin affects the secretion of proteases in Mannheimia haemolytica A2
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Gerardo Ramírez-Rico, Mireya de la Garza, Christian Avalos-Gómez, and Moises Martinez-Castillo
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Proteases ,Protease ,biology ,Chemistry ,Lactoferrin ,medicine.medical_treatment ,Virulence ,zymography ,biology.organism_classification ,Virulence factor ,Microbiology ,lactoferrin ,biology.protein ,medicine ,General Materials Science ,Secretion ,proteases ,Bacterial outer membrane ,Mannheimia haemolytica ,Bacteria ,Research Articles - Abstract
Mannheimia haemolytica serotype A2 is the main bacterial causative agent of ovine mannheimiosis, a disease that leads to substantial economic losses for livestock farmers. Several virulence factors allow M. haemolytica to colonize the lungs and establish infection. Virulence factors can be directly secreted into the environment by bacteria but are also released through outer membrane vesicles (OMVs). In addition, due to the abuse of antibiotics in the treatment of this disease, multidrug-resistant bacterial strains of M. haemolytica have emerged. One therapeutic alternative to antibiotics or an adjuvant to be used in combination with antibiotics could be lactoferrin (Lf), a multifunctional cationic glycoprotein of the mammalian innate immune system to which no bacterial resistance has been reported. The aim of this work was to determine the effect of bovine iron-free Lf (apo-BLf) on the production and secretion of proteases into culture supernatant (CS) and on their release in OMVs. Zymography assays showed that addition of sub-MIC concentrations of apo-BLf to M. haemolytica cultures inhibited protease secretion without affecting culture growth. Biochemical characterization revealed that these proteases were mainly cysteine- and metalloproteases. The secretion of a 100 kDa metalloprotease was inhibited by sub-MIC concentrations of apo-BLf since this protease was present in the cytoplasm and OMVs but not in CS proteins, as corroborated by Western blotting. On the other hand, proteases produced by M. haemolytica caused cleavage of apo-BLf. However, when Lf is cleaved, peptides known as lactoferricins, which are more bactericidal than natural Lf, can be produced. M. haemolytica A2 protease-mediated degradation of host tissue proteins could be an important virulence factor during the infectious process of pneumonia in ovines. The mechanism of M. haemolytica protease secretion could be inhibited by treatment with apo-BLf in animals.
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- 2021
39. Pregnancy, Birth, Infant, and Early Childhood Neurodevelopmental Outcomes among a Cohort of Women with Symptoms of Zika Virus Disease during Pregnancy in Three Surveillance Sites, Project Vigilancia de Embarazadas con Zika (VEZ), Colombia, 2016–2018
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Kayla N. Anderson, Johana Osorio, Jacob Hojnacki, Veronica K. Burkel, Sarah Reagan-Steiner, Angelica Rico, Maritza Gonzalez, Evelene Steward-Clark, Diana Valencia, Shana Godfred-Cato, Jonathan Daniels, Marcela Mercado-Reyes, Julu Bhatnagar, Sarah C. Tinker, Julie Villanueva, Romeo R. Galang, Christina M Winfield, Suzanne M. Gilboa, Jessica N Ricaldi, Mónica Benavides, Cynthia A. Moore, Marcela Daza, Lissethe Pardo, Miranda J Delahoy, Jarad Schiffer, Jennifer Dolan Thomas, Sherif R. Zaki, Martha Lucia Ospina Martinez, May Bibiana Osorio, Christina L Sancken, Van T. Tong, and Margaret A. Honein
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Zika virus disease ,medicine.medical_specialty ,Microcephaly ,Zika virus ,pregnancy ,Colombia ,birth defects ,neurodevelopment ,Article ,medicine ,Early childhood ,Fetus ,Pregnancy ,General Immunology and Microbiology ,biology ,Obstetrics ,business.industry ,Medical record ,Public Health, Environmental and Occupational Health ,biology.organism_classification ,medicine.disease ,Infectious Diseases ,Cohort ,business - Abstract
Project Vigilancia de Embarazadas con Zika (VEZ), an intensified surveillance of pregnant women with symptoms of the Zika virus disease (ZVD) in Colombia, aimed to evaluate the relationship between symptoms of ZVD during pregnancy and adverse pregnancy, birth, and infant outcomes and early childhood neurodevelopmental outcomes. During May–November 2016, pregnant women in three Colombian cities who were reported with symptoms of ZVD to the national surveillance system, or with symptoms of ZVD visiting participating clinics, were enrolled in Project VEZ. Data from maternal and pediatric (up to two years of age) medical records were abstracted. Available maternal specimens were tested for the presence of the Zika virus ribonucleic acid and/or anti-Zika virus immunoglobulin antibodies. Of 1213 enrolled pregnant women with symptoms of ZVD, 1180 had a known pregnancy outcome. Results of the Zika virus laboratory testing were available for 569 (48.2%) pregnancies with a known pregnancy outcome though testing timing varied and was often distal to the timing of symptoms; 254 (21.5% of the whole cohort; 44.6% of those with testing results) were confirmed or presumptive positive for the Zika virus infection. Of pregnancies with a known outcome, 50 (4.2%) fetuses/infants had Zika-associated brain or eye defects, which included microcephaly at birth. Early childhood adverse neurodevelopmental outcomes were more common among those with Zika-associated birth defects than among those without and more common among those with laboratory evidence of a Zika virus infection compared with the full cohort. The proportion of fetuses/infants with any Zika-associated brain or eye defect was consistent with the proportion seen in other studies. Enhancements to Colombia’s existing national surveillance enabled the assessment of adverse outcomes associated with ZVD in pregnancy.
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- 2021
40. Differential Regulation of the Asthmatic Phenotype by the Aryl Hydrocarbon Receptor
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Hussein Traboulsi, Angela Rico de Souza, Benoit Allard, Zahraa Haidar, Mark Sorin, Vanessa Moarbes, Elizabeth D. Fixman, James G. Martin, David H. Eidelman, and Carolyn J. Baglole
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Physiology ,Inflammation ,Immune system ,neutrophils ,Physiology (medical) ,medicine ,Eosinophilia ,QP1-981 ,Transcription factor ,lungs ,Original Research ,Asthma ,biology ,business.industry ,aryl hydrocarbon receptor ,respiratory system ,medicine.disease ,Aryl hydrocarbon receptor ,respiratory tract diseases ,Ovalbumin ,inflammation ,chlorine ,Immunology ,biology.protein ,medicine.symptom ,occupational asthma ,business ,Occupational asthma ,allergic asthma - Abstract
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that regulates the metabolism of xenobiotics. There is growing evidence that the AhR is implicated in physiological processes such proliferation, differentiation, and immune responses. Recently, a role of the AhR in regulating allergic asthma has been suggested, but whether the AhR also regulates other type of asthma, particularly occupational/irritant-induced asthma, remains unknown. Using AhR-deficient (Ahr−/−) mice, we compared the function of the AhR in the response to ovalbumin (OVA; allergic asthma) vs. chlorine (Cl2; irritant-induced asthma) exposure. Lung inflammation and airway hyperresponsiveness were assessed 24h after exposure to Cl2 or OVA challenge in Ahr−/− and heterozygous (Ahr+/−) mice. After OVA challenge, absence of AhR was associated with significantly enhanced eosinophilia and lymphocyte influx into the airways of Ahr−/− mice. There were also increased levels of interleukin-4 (IL-4) and IL-5 in the airways. However, OVA-induced airway hyperresponsiveness was not affected. In the irritant-induced asthma model caused by exposure to Cl2, the AhR did not regulate the inflammatory response. However, absence of AhR reduced Cl2-induced airway hyperresponsiveness. Collectively, these results support a differential role for the AhR in regulating asthma outcomes in response to diverse etiological agents.
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- 2021
41. Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
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Darío García de Viedma, Laura Pérez-Lago, Carla Rico-Luna, Ana Candela, Pilar Catalán, Álvaro Otero-Sobrino, Patricia Muñoz, Andrea Molero-Salinas, Carmen Losada, and Sergio Buenestado-Serrano
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Microbiology (medical) ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Physiology ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,RT-PCR ,Observation ,Genome, Viral ,Biology ,Microbiology ,reverse transcriptase PCR ,Genetics ,Humans ,Diagnostic Errors ,Whole genome sequencing ,General Immunology and Microbiology ,Ecology ,Whole Genome Sequencing ,Reverse Transcriptase Polymerase Chain Reaction ,SARS-CoV-2 ,VOC ,COVID-19 ,Cell Biology ,QR1-502 ,Reverse transcription polymerase chain reaction ,Infectious Diseases ,Real-time polymerase chain reaction ,whole-genome sequencing ,COVID-19 Nucleic Acid Testing ,Spike Glycoprotein, Coronavirus ,WGS - Abstract
Reverse transcription-PCRs (RT-PCRs) targeting SARS-CoV-2 variant of concern (VOC) mutations have been developed to simplify their tracking. We evaluated an assay targeting E484K/N501Y to identify B.1.351/P1. Whole-genome sequencing (WGS) confirmed only 72 (59.02%) of 122 consecutive RT-PCR P.1/B.1.351 candidates. Prescreening RT-PCRs must target a wider set of mutations, updated from WGS data from emerging variants.
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- 2021
42. Levels of Salmonella enterica and Listeria monocytogenes in Alternative Irrigation Water Vary Based on Water Source on the Eastern Shore of Maryland
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Sultana Solaiman, Chanelle L Acheamfour, Adam Vanore, Fawzy M. Hashem, Rianna Murray, Megan E. Gerdes, Samantha Gartley, Rachel E. Rosenberg Goldstein, Manan Sharma, Shirley A. Micallef, Kalmia E. Kniel, Seongyun Kim, Shani Craighead, Derek Foust, Eric B. May, Eric T. Handy, Cheryl East, Anthony Bui, Hillary A. Craddock, Maryam Taabodi, Rhodel Bradshaw, Prachi Kulkarni, Sarah M. Allard, Joseph Haymaker, Amy R. Sapkota, Koriante Rogers, Brienna L. Anderson-Coughlin, Mary Theresa Callahan, Salina Parveen, and Rico Duncan
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Microbiology (medical) ,Veterinary medicine ,Salmonella ,Irrigation ,Agricultural Irrigation ,Physiology ,mid-Atlantic ,Fresh Water ,medicine.disease_cause ,Microbiology ,irrigation ,Water scarcity ,Most probable number ,Genetics ,medicine ,reclaimed water ,filtration ,General Immunology and Microbiology ,Ecology ,Brackish water ,biology ,Maryland ,Salmonella enterica ,Water ,Cell Biology ,biology.organism_classification ,Listeria monocytogenes ,Reclaimed water ,QR1-502 ,Infectious Diseases ,Environmental science ,modified Moore swab ,Water Microbiology ,Groundwater ,Research Article - Abstract
Irrigation water sources have been shown to harbor foodborne pathogens and could contribute to the outbreak of foodborne illness related to consumption of contaminated produce. Determining the probability of and the degree to which these irrigation water sources contain these pathogens is paramount. The purpose of this study was to determine the prevalence of Salmonella enterica and Listeria monocytogenes in alternative irrigation water sources. Water samples (n = 188) were collected over 2 years (2016 to 2018) from 2 reclaimed water plants, 3 nontidal freshwater rivers, and 1 tidal brackish river on Maryland’s Eastern Shore (ESM). Samples were collected by filtration using modified Moore swabs (MMS) and analyzed by culture methods. Pathogen levels were quantified using a modified most probable number (MPN) procedure with three different volumes (10 liters, 1 liter, and 0.1 liter). Overall, 65% (122/188) and 40% (76/188) of water samples were positive for S. enterica and L. monocytogenes, respectively. For both pathogens, MPN values ranged from 0.015 to 11 MPN/liter. Pathogen levels (MPN/liter) were significantly (P
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- 2021
43. Genetic insights into biological mechanisms governing human ovarian ageing
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Unnur Styrkarsdottir, Lynda M. Rose, Rehannah Borup, Anne B. Newman, Georgia Chenevix-Trench, Chikashi Terao, Jeremy A. Daniel, Christa Meisinger, Albert V. Smith, Emil Peter Thrane Hertz, Raymond Noordam, Wei He, Jennifer A. Smith, Mikael Eriksson, Konstantin Strauch, Daniel I. Chasman, Nicholas J. Timpson, Melissa A. Troester, Claudia Langenberg, Montserrat Garcia-Closas, Mohammad Arfan Ikram, Sara Lindström, Gad Rennert, Pascal Guénel, Kristina W. Olsen, Pierre Fontanillas, Ozren Polasek, Daniel F. Gudbjartsson, Frank B. Hu, Archie Campbell, Celine M. Vachon, Sheila Ulivi, Robin N Beaumont, Robert Karlsson, Lenore J. Launer, Renée de Mutsert, Annette Peters, David Schlessinger, Stefania Bandinelli, Rico Rueedi, Joop S.E. Laven, Pascal Timshel, Joanne M. Murabito, Kuang Lin, Jazib Hussain, Dennis O. Mook-Kanamori, Manjeet K. Bolla, Catherine E. Aiken, Javier Martin Gonzalez, Simon S. Cross, Immaculata De Vivo, Paul M. Ridker, Christopher A. Haiman, Gerardo Heiss, Jessica Tyrrell, Paul R. H. J. Timmers, Hironori Abe, Mike A. Nalls, Luigi Ferrucci, Natalia Perjakova, Jouke J. Hottenga, Robin G. Walters, Reedik Mägi, Niclas Håkansson, Miriam Dwek, Barbara McKnight, Sandra Turon, Stasa Stankovic, Linda Broer, Stephen J. Chanock, Martina La Bianca, Jenny Chang-Claude, Loic Le Marchand, Hamdi Mbarek, Doris Stöckl, Andrew F. Olshan, Graham G. Giles, James F. Wilson, Micaella Joaquim, Amruta Shrikhande, Eva Hoffmann, Stefania Benonisdottir, Ana Martínez-Marchal, Anthony J. Swerdlow, David Karasik, Nicholas J. Wareham, Peter A. Fasching, Jane L. Tarry-Adkins, Charles Kooperberg, Peter Vollenweider, Douglas F. Easton, Paula Aguilera, Jessica D. Faul, Patrik K. E. Magnusson, Emmanouil Saloustros, Alpa V. Patel, Ellen W. Demerath, Qin Wang, Aditya Sankar, Christopher G. Scott, Iffat Rahman, Sharon L.R. Kardia, Peter K. Joshi, Caterina Barbieri, Claus Yding Andersen, Tõnu Esko, Massimo Mezzavilla, Nicholas G. Martin, Rebecca D. Jackson, Alison D. Murray, Marina Ciullo, Nicholas Bowker, Anna Murray, Patrick Deelen, Zoltán Kutalik, Alicja Wolk, Manuela Gago-Dominguez, Eleonora Porcu, Laura Crisponi, Michela Traglia, Katharina E. Schraut, Antonietta Robino, Chunyan He, Bruce H. R. Wolffenbuttel, Henry Völzke, Daniela Ruggiero, John R. B. Perry, Lude Franke, Igor Rudan, Angela Cox, Unnur Þorsteinsdottir, Christian Gieger, David R. Weir, Jodie N. Painter, Martha S. Linet, Massimo Mangino, Melissa C. Southey, Petr Solc, Tim D. Spector, Christiana Kartsonaki, Momoko Horikoshi, Meir J. Stampfer, Eulalia Catamo, Mònica Ferrer-Roda, Ko Willems van Dijk, Daniela Toniolo, Caroline Hayward, Lili Milani, Chloé Sarnowski, Jian'an Luan, Behrooz Z. Alizadeh, Jenny A. Visser, Stig E. Bojesen, Genevieve Lachance, Ulrike Peters, Antonella Mulas, John J. Spinelli, Elnaz Naderi, Andrew R. Wood, Paul D.P. Pharoah, Elinor J. Sawyer, Annique Claringbould, Saleh Shekari, David G. Hunter, Marie Louise Grøndahl, Vilmundur Gudnason, Nora Franceschini, Dale P. Sandler, Dale R. Nyholt, Jacques E. Rossouw, Amber N. Wilcox, Thomas U. Ahearn, Hedy S. Rennert, Olivier B. Bakker, Jingmei Li, Francesco Cucca, Eric Boerwinkle, Matthias W. Beckmann, Cristina Menni, Minouk J. Schoemaker, Esther M. John, Tune H. Pers, Andrés J. López-Contreras, Tanguy Corre, Jonathan Marten, Alice M. Arnold, N. Charlotte Onland-Moret, Lucie Knoblochova, Anna Pujol, Kathryn L. Lunetta, Marjanka K. Schmidt, Teresa Nutile, Serena Sanna, Gonneke Willemsen, Roger L. Milne, Kristan J. Aronson, Frits R. Rosendaal, Murielle Bochud, Ken K. Ong, Susan M. Ring, Nancy L. Pedersen, Blair H. Smith, Ivana Kolcic, Annelie Augustinsson, Jose E. Castelao, Alexander Teumer, Felix R. Day, Sven Bergmann, Timothy M. Frayling, Lauren R. Teras, George Davey Smith, Thomas Meitinger, Alison M. Dunning, Ignasi Roig, Dorret I. Boomsma, Harald Grallert, Toshiko Tanaka, Katherine S. Ruth, Julie E. Buring, Marek Zygmunt, Uwe Völker, Irene L. Andrulis, Håkan Olsson, Harry Campbell, Cari M. Kitahara, Annika Lindblom, Yvonne T. van der Schouw, Cinzia Sala, Debbie A Lawlor, Joe Dennis, Yongmei Liu, Yan Huang, Stephen Burgess, Brumat Marco, Veronique Vitart, Kari Stefansson, Susan E. Ozanne, Kamila Czene, Simin Liu, John L. Hopper, Joyce B. J. van Meurs, Satoshi H. Namekawa, Miya Kudo Høffding, Fergus J. Couch, Ajuna Azad, Eco J. C. de Geus, Liming Li, Grant W. Montgomery, Peter Kraft, André G. Uitterlinden, Arto Mannermaa, Heiko Becher, Allison W. Kurian, Vallari Shukla, Zhengming Chen, Per Hall, Jennifer A. Brody, Rossella Sorice, Wei Zhao, Andres Metspalu, Sarah E. Medland, Tricia Lindstrom, Clarice R. Weinberg, Bruce M. Psaty, Thérèse Truong, Anna Marie Mulligan, Deborah J. Thompson, Patrick Sulem, Internal Medicine, Epidemiology, Obstetrics & Gynecology, Hoffmann, Eva R [0000-0002-2588-0652], Murray, Anna [0000-0002-2351-2522], Roig, Ignasi [0000-0003-0313-3581], Perry, John RB [0000-0001-6483-3771], Apollo - University of Cambridge Repository, Life Course Epidemiology (LCE), Real World Studies in PharmacoEpidemiology, -Genetics, -Economics and -Therapy (PEGET), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Center for Liver, Digestive and Metabolic Diseases (CLDM), Stem Cell Aging Leukemia and Lymphoma (SALL), Biological Psychology, APH - Mental Health, APH - Methodology, APH - Personalized Medicine, and APH - Health Behaviors & Chronic Diseases
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Gerontology ,Aging ,Far East ,Endocrinology, Diabetes and Metabolism ,Menopause, Premature ,Genome-wide association study ,VARIANTS ,Primary Ovarian Insufficiency ,Inbred C57BL ,Bioinformatics ,DISEASE ,Healthy Aging ,genetics of ovarian aging ,Fragile X Mental Retardation Protein ,Mice ,Endocrinology ,Medicine ,EARLY MENOPAUSE ,media_common ,RISK ,Multidisciplinary ,Asia, Eastern ,Reproduction ,Longevity ,Middle Aged ,Europe ,MENDELIAN RANDOMIZATION ,Medical genetics ,Female ,ICEP ,Menopause ,Adult ,Alleles ,Animals ,Bone and Bones ,Checkpoint Kinase 1 ,Checkpoint Kinase 2 ,Diabetes Mellitus, Type 2 ,Diet ,Fertility ,Genetic Predisposition to Disease ,Genome-Wide Association Study ,Humans ,Mice, Inbred C57BL ,Ovary ,Uterus ,Type 2 ,medicine.medical_specialty ,media_common.quotation_subject ,MEDLINE ,Biology ,Premature ovarian insufficiency ,Article ,genome-wide meta-analysis ,SDG 3 - Good Health and Well-being ,GERMLINE ,Diabetes Mellitus ,Genetic predisposition ,Ovarian reserve ,Premature ,business.industry ,Human genetics ,CHROMOSOME SYNAPSIS ,DNA-DAMAGE ,Ageing ,EXPRESSION ANALYSIS ,business ,MEIOTIC CELL-CYCLE - Abstract
Reproductive longevity is essential for fertility and influences healthy ageing in women1,2, but insights into its underlying biological mechanisms and treatments to preserve it are limited. Here we identify 290 genetic determinants of ovarian ageing, assessed using normal variation in age at natural menopause (ANM) in about 200,000 women of European ancestry. These common alleles were associated with clinical extremes of ANM; women in the top 1% of genetic susceptibility have an equivalent risk of premature ovarian insufficiency to those carrying monogenic FMR1 premutations3. The identified loci implicate a broad range of DNA damage response (DDR) processes and include loss-of-function variants in key DDR-associated genes. Integration with experimental models demonstrates that these DDR processes act across the life-course to shape the ovarian reserve and its rate of depletion. Furthermore, we demonstrate that experimental manipulation of DDR pathways highlighted by human genetics increases fertility and extends reproductive life in mice. Causal inference analyses using the identified genetic variants indicate that extending reproductive life in women improves bone health and reduces risk of type 2 diabetes, but increases the risk of hormone-sensitive cancers. These findings provide insight into the mechanisms that govern ovarian ageing, when they act, and how they might be targeted by therapeutic approaches to extend fertility and prevent disease. Hundreds of genetic loci associated with age at menopause, combined with experimental evidence in mice, highlight mechanisms of reproductive ageing across the lifespan.
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- 2021
44. Pumilio2 Promotes Growth of Mature Neurons
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Michael A. Kiebler, Bastian Popper, Barbara Saccà, Max Harner, Elisa-Charlott Schöneweiss, Rico Schieweck, Daniela Rieger, and Christin Illig
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neuronal growth ,Male ,QH301-705.5 ,medicine.medical_treatment ,Eukaryotic Initiation Factor-4E ,Neurogenesis ,Basic fibroblast growth factor ,Medizin ,Gene Expression ,Biology ,Microscopy, Atomic Force ,Catalysis ,Article ,Inorganic Chemistry ,Transcriptome ,chemistry.chemical_compound ,Mice ,medicine ,Animals ,RNA, Messenger ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Spectroscopy ,Neurons ,atomic force microscopy ,Cell growth ,Growth factor ,Organic Chemistry ,EIF4E ,Pumilio2 ,RNA-Binding Proteins ,Translation (biology) ,General Medicine ,Computer Science Applications ,Cell biology ,Mice, Inbred C57BL ,Chemistry ,chemistry ,eIF4E ,Cancer cell ,Female ,Fibroblast Growth Factor 2 ,Protein Binding - Abstract
RNA-binding proteins (RBPs) are essential regulators controlling both the cellular transcriptome and translatome. These processes enable cellular plasticity, an important prerequisite for growth. Cellular growth is a complex, tightly controlled process. Using cancer cells as model, we looked for RBPs displaying strong expression in published transcriptome datasets. Interestingly, we found the Pumilio (Pum) protein family to be highly expressed in all these cells. Moreover, we observed that Pum2 is regulated by basic fibroblast growth factor (bFGF). bFGF selectively enhances protein levels of Pum2 and the eukaryotic initiation factor 4E (eIF4E). Exploiting atomic force microscopy and in vitro pulldown assays, we show that Pum2 selects for eIF4E mRNA binding. Loss of Pum2 reduces eIF4E translation. Accordingly, depletion of Pum2 led to decreased soma size and dendritic branching of mature neurons, which was accompanied by a reduction in essential growth factors. In conclusion, we identify Pum2 as an important growth factor for mature neurons. Consequently, it is tempting to speculate that Pum2 may promote cancer growth.
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- 2021
45. Functional Characterization of 21 Rare Allelic CYP1A2 Variants Identified in a Population of 4773 Japanese Individuals by Assessing Phenacetin O-Deethylation
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Akifumi Oda, Tomoki Nakayoshi, Shuki Yamazaki, Akiko Ueda, Shu Tadaka, Daisuke Saigusa, Eiji Hishinuma, Sakae Saito, Masahiro Hiratsuka, Evelyn Marie Gutiérrez Rico, Masaki Kumondai, Kengo Kinoshita, Yuya Nakanishi, and Noriyasu Hirasawa
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0301 basic medicine ,Population ,Medicine (miscellaneous) ,Biology ,030226 pharmacology & pharmacy ,Article ,03 medical and health sciences ,0302 clinical medicine ,Genetic variation ,medicine ,Allele ,education ,Genetics ,education.field_of_study ,CYP1A2 ,Phenotype ,drug metabolism ,030104 developmental biology ,Docking (molecular) ,Phenacetin ,genetic variation ,phenacetin ,Medicine ,cytochrome P450 1A2 ,Drug metabolism ,medicine.drug - Abstract
Cytochrome P450 1A2 (CYP1A2), which accounts for approximately 13% of the total hepatic cytochrome content, catalyzes the metabolic reactions of approximately 9% of frequently used drugs, including theophylline and olanzapine. Substantial inter-individual differences in enzymatic activity have been observed among patients, which could be caused by genetic polymorphisms. Therefore, we functionally characterized 21 novel CYP1A2 variants identified in 4773 Japanese individuals by determining the kinetic parameters of phenacetin O-deethylation. Our results showed that most of the evaluated variants exhibited decreased or no enzymatic activity, which may be attributed to potential structural alterations. Notably, the Leu98Gln, Gly233Arg, Ser380del Gly454Asp, and Arg457Trp variants did not exhibit quantifiable enzymatic activity. Additionally, three-dimensional (3D) docking analyses were performed to further understand the underlying mechanisms behind variant pharmacokinetics. Our data further suggest that despite mutations occurring on the protein surface, accumulating interactions could result in the impairment of protein function through the destabilization of binding regions and changes in protein folding. Therefore, our findings provide additional information regarding rare CYP1A2 genetic variants and how their underlying effects could clarify discrepancies noted in previous phenotypical studies. This would allow the improvement of personalized therapeutics and highlight the importance of identifying and characterizing rare variants.
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- 2021
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46. Comparative Analysis of the Filling Capacity of Simulated Lateral Canals of Two Bioactive Root Canal Sealers Using Warm Gutta-Percha Technique
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Jesús Mena-Álvarez, Cristina Rico-Romano, Juliette Theophanie Sevrain, and Mario Zorita-García
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Technology ,Materials science ,QH301-705.5 ,Root canal ,QC1-999 ,warm obturation ,Dentistry ,Linear measurement ,root canal sealers ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine ,General Materials Science ,Biology (General) ,Instrumentation ,QD1-999 ,simulated lateral canals ,Fluid Flow and Transfer Processes ,biology ,business.industry ,Process Chemistry and Technology ,Physics ,General Engineering ,030206 dentistry ,Gutta-percha ,biology.organism_classification ,Engineering (General). Civil engineering (General) ,Computer Science Applications ,Apex (geometry) ,Chemistry ,medicine.anatomical_structure ,extrusion ,Coronal plane ,bioceramics ,TA1-2040 ,business - Abstract
The aim of this study was to evaluate ex vivo the sealing achieved at simulated lateral canals (SLC) and the quality of filling according to their position in the root canal after using the same filling technique. SLC were created at three levels in 55 teeth and divided into two groups depending on the root canal sealer used (1: BioRoot® RCS, 2: GuttaFlow® bioseal). They filled them with the continuous wave technique and submitted to a diaphanization technique. The samples were analyzed using a magnifying lens (20×), pictures were taken, which proceeded to linear measurement with the ImageJ® program and used a filling score system with five grades (0 to 4, 0 and 1 not acceptable, 2 to 4 acceptable), BioRoot® RCS has got a greater proportion than GuttaFlow® bioseal for SLC filled acceptably at 10 mm from the apex (p <, 0.05). The highest proportion of SLC filled acceptably was found in the middle third (6 mm) (p <, 0.05), followed by the apical third (3 mm) and the coronal third (10 mm). The difference between apical and coronal third could be significant, BioRoot® RCS has been better than GuttaFlow® bioseal for filling SLC in the coronal third of the teeth. Studies on the characteristics of these cements are missing to explain these differences.
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- 2021
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47. Deep Phenotyping and Genetic Characterization of a Cohort of 70 Individuals With 5p Minus Syndrome
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Julián Nevado, Cristina Bel-Fenellós, Ana Karen Sandoval-Talamantes, Adolfo Hernández, Chantal Biencinto-López, María Luisa Martínez-Fernández, Pilar Barrúz, Fernando Santos-Simarro, María Ángeles Mori-Álvarez, Elena Mansilla, Fé Amalia García-Santiago, Isabel Valcorba, Belén Sáenz-Rico, María Luisa Martínez-Frías, and Pablo Lapunzina
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0301 basic medicine ,Microcephaly ,Cri du chat ,Cri du Chat Syndrome ,QH426-470 ,030105 genetics & heredity ,Biology ,Intellectual disabilities ,03 medical and health sciences ,Neurodevelopmental disorder ,Behavior problems ,medicine ,Genetics ,SNP ,Global developmental delay ,Genetics (clinical) ,5p-minus syndrome ,Original Research ,Genetic heterogeneity ,medicine.disease ,behavior problems ,030104 developmental biology ,Cohort ,Subtelomeric deletion ,subtelomeric deletion ,Molecular Medicine ,intellectual disabilities - Abstract
Chromosome-5p minus syndrome (5p-Sd, OMIM #123450) formerly known as Cri du Chat syndrome results from the loss of genetic material at the distal region of the short arm of chromosome 5. It is a neurodevelopmental disorder of genetic cause. So far, about 400 patients have been reported worldwide. Individuals affected by this syndrome have large phenotypic heterogeneity. However, a specific phenotype has emerged including global developmental delay, microcephaly, delayed speech, some dysmorphic features, and a characteristic and monochromatic high-pitch voice, resembling a cat’s cry. We here describe a cohort of 70 patients with clinical features of 5p- Sd characterized by means of deep phenotyping, SNP arrays, and other genetic approaches. Individuals have a great clinical and molecular heterogeneity, which can be partially explained by the existence of additional significant genomic rearrangements in around 39% of cases. Thus, our data showed significant statistical differences between subpopulations (simple 5p deletions versus 5p deletions plus additional rearrangements) of the cohort. We also determined significant “functional” differences between male and female individuals.
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- 2021
48. Genome-wide association analyses of risk tolerance and risky behaviors in over 1 million individuals identify hundreds of loci and shared genetic influences
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Karlsson Linnér, Richard, Biroli, Pietro, Kong, Edward, Meddens, S. Fleur W., Wedow, Robbee, Fontana, Mark Alan, Lebreton, Maël, Tino, Stephen P., Abdellaoui, Abdel, Hammerschlag, Anke R., Nivard, Michel G., Okbay, Aysu, Rietveld, Cornelius A., Timshel, Pascal N., Trzaskowski, Maciej, Vlaming, Ronald de, Zünd, Christian L., Bao, Yanchun, Buzdugan, Laura, Caplin, Ann H., Chen, Chia-Yen, Eibich, Peter, Fontanillas, Pierre, Gonzalez, Juan R., Joshi, Peter K., Karhunen, Ville, Kleinman, Aaron, Levin, Remy Z., Lill, Christina M., Meddens, Gerardus A., Muntané, Gerard, Sanchez-Roige, Sandra, Rooij, Frank J. van, Taskesen, Erdogan, Yang, Wu, Zhang, Futao, Agee, Michelle, Alipanahi, Babak, Bell, Robert K., Bryc, Katarzyna, Elson, Sarah L., Furlotte, Nicholas A., Huber, Karen E., Litterman, Nadia K., Mccreight, Jennifer C., Mcintyre, Matthew H., Mountain, Joanna L., Northover, Carrie A. M., Pitts, Steven J., Sathirapongsasuti, J. Fah, Sazonova, Olga V., Shelton, Janie F., Shringarpure, Suyash, Tian, Chao, Tung, Joyce Y., Vacic, Vladimir, Wilson, Catherine H., Agbessi, Mawussé, Ahsan, Habibul, Alves, Isabel, Andiappan, Anand, Awadalla, Philip, Battle, Alexis, Beutner, Frank, Jan Bonder, Marc, Boomsma, Dorret I., Christiansen, Mark, Claringbould, Annique, Deelen, Patrick, Esko, Tõnu, Favé, Marie-Julie, Franke, Lude, Frayling, Timothy, Gharib, Sina A., Gibson, Gregory, Heijmans, Bastiaan, Hemani, Gibran, Jansen, Rick, Kähönen, Mika, Kalnapenkis, Anette, Kasela, Silva, Kettunen, Johannes, Kim, Yungil, Kirsten, Holger, Kovacs, Peter, Krohn, Knut, Kronberg-Guzman, Jaanika, Kukushkina, Viktorija, Kutalik, Zoltan, Lee, Bernett, Lehtimäki, Terho, Loeffler, Markus, Marigorta, Urko M., Metspalu, Andres, Milani, Lili, Montgomery, Grant W., Müller-Nurasyid, Martina, Nauck, Matthias, Penninx, Brenda, Perola, Markus, Pervjakova, Natalia, Pierce, Brandon, Powell, Joseph, Prokisch, Holger, Psaty, Bruce M., Raitakari, Olli, Ring, Susan, Ripatti, Samuli, Rotzchke, Olaf, Rüeger, Sina, Saha, Ashis, Scholz, Markus, Schramm, Katharina, Seppälä, Ilkka, Stumvoll, Michael, Sullivan, Patrick, Hoen, Peter-Bram t, Teumer, Alexander, Thiery, Joachim, Tong, Lin, Tönjes, Anke, Dongen, Jenny van, Meurs, Joyce van, Verlouw, Joost, Visscher, Peter M., Völker, Uwe, Võsa, Urmo, Westra, Harm-Jan, Yaghootkar, Hanieh, Yang, Jian, Zeng, Biao, Beauchamp, Jonathan P., Lee, James J., Pers, Tune H., Turley, Patrick, Chen, Guo-Bo, Emilsson, Valur, Oskarsson, Sven, Pickrell, Joseph K., Thom, Kevin, Timshel, Pascal, de Vlaming, Ronald, Ahluwalia, Tarunveer S., Bacelis, Jonas, Baumbach, Clemens, Bjornsdottir, Gyda, Brandsma, Johannes H., Concas, Maria Pina, Derringer, Jaime, Galesloot, Tessel E., Girotto, Giorgia, Gupta, Richa, Hall, Leanne M., Harris, Sarah E., Hofer, Edith, Horikoshi, Momoko, Huffman, Jennifer E., Kaasik, Kadri, Kalafati, Ioanna P., Karlsson, Robert, Kong, Augustine, Lahti, Jari, Lee, Sven J. van der, de Leeuw, Christiaan, Lind, Penelope A., Lindgren, Karl-Oskar, Liu, Tian, Mangino, Massimo, Marten, Jonathan, Mihailov, Evelin, Miller, Michael B., Most, Peter J. van der, Oldmeadow, Christopher, Payton, Antony, Peyrot, Wouter J., Qian, Yong, Rueedi, Rico, Salvi, Erika, Schmidt, Börge, Schraut, Katharina E., Shi, Jianxin, Smith, Albert V., Poot, Raymond A., Pourcain, Beate St, Thorleifsson, Gudmar, Verweij, Niek, Vuckovic, Dragana, Wellmann, Juergen, Yang, Jingyun, Zhao, Wei, Zhu, Zhihong, Alizadeh, Behrooz Z., Amin, Najaf, Bakshi, Andrew, Baumeister, Sebastian E., Biino, Ginevra, Bønnelykke, Klaus, Boyle, Patricia A., Campbell, Harry, Cappuccio, Francesco P., Davies, Gail, De Neve, Jan-Emmanuel, Deloukas, Panos, Demuth, Ilja, Ding, Jun, Eisele, Lewin, Eklund, Niina, Evans, David M., Faul, Jessica D., Feitosa, Mary F., Forstner, Andreas J., Gandin, Ilaria, Gunnarsson, Bjarni, Halldórsson, Bjarni V., Harris, Tamara B., Heath, Andrew C., Hocking, Lynne J., Holliday, Elizabeth G., Homuth, Georg, Horan, Michael A., Hottenga, Jouke-Jan, de Jager, Philip L., Jugessur, Astanand, Kaakinen, Marika A., Kanoni, Stavroula, Keltigangas-Järvinen, Liisa, Kiemeney, Lambertus A. L. M., Kolcic, Ivana, Koskinen, Seppo, Kraja, Aldi T., Kroh, Martin, Latvala, Antti, Launer, Lenore J., Lebreton, Maël P., Levinson, Douglas F., Lichtenstein, Paul, Lichtner, Peter, Liewald, David C. M., Loukola, Anu, Madden, Pamela A., Mägi, Reedik, Mäki-Opas, Tomi, Marioni, Riccardo E., Marques-Vidal, Pedro, Mcmahon, George, Meisinger, Christa, Meitinger, Thomas, Milaneschi, Yusplitri, Myhre, Ronny, Nelson, Christopher P., Nyholt, Dale R., Ollier, William E. R., Palotie, Aarno, Paternoster, Lavinia, Pedersen, Nancy L., Petrovic, Katja E., Porteous, David J., Räikkönen, Katri, Ring, Susan M., Robino, Antonietta, Rostapshova, Olga, Rudan, Igor, Rustichini, Aldo, Salomaa, Veikko, Sanders, Alan R., Sarin, Antti-Pekka, Schmidt, Helena, Scott, Rodney J., Smith, Blair H., Smith, Jennifer A., Staessen, Jan A., Steinhagen-Thiessen, Elisabeth, Strauch, Konstantin, Terracciano, Antonio, Tobin, Martin D., Ulivi, Sheila, Vaccargiu, Simona, Quaye, Lydia, Venturini, Cristina, Vinkhuyzen, Anna A. E., Völzke, Henry, Vonk, Judith M., Vozzi, Diego, Waage, Johannes, Ware, Erin B., Willemsen, Gonneke, Attia, John R., Bennett, David A., Berger, Klaus, Bertram, Lars, Bisgaard, Hans, Borecki, Ingrid B, Bültmann, Ute, Chabris, Christopher F., Cucca, Francesco, Cusi, Daniele, Deary, Ian J., Dedoussis, George V., Duijn, Cornelia M. van, Eriksson, Johan G., Franke, Barbara, Gasparini, Paolo, Gejman, Pablo V., Gieger, Christian, Grabe, Hans-Jörgen, Gratten, Jacob, Groenen, Patrick J. F., Gudnason, Vilmundur, Harst, Pim van der, Hayward, Caroline, Hinds, David A., Hoffmann, Wolfgang, Hyppönen, Elina, Iacono, William G., Jacobsson, Bo, Järvelin, Marjo-Riitta, Jöckel, Karl-Heinz, Kaprio, Jaakko, Kardia, Sharon L. R., Lehrer, Steven F., Magnusson, Patrik K. E., Martin, Nicholas G., Mcgue, Matt, Pendleton, Neil, Pirastu, Nicola, Pirastu, Mario, Polasek, Ozren, Posthuma, Danielle, Power, Christine, Province, Michael A., Samani, Nilesh J., Schlessinger, David, Schmidt, Reinhold, Sørensen, Thorkild I. A., Spector, Tim D., Stefansson, Kari, Thorsteinsdottir, Unnur, Thurik, A. Roy, Timpson, Nicholas J., Tiemeier, Henning, Uitterlinden, André G., Vitart, Veronique, Vollenweider, Peter, Weir, David R., Wilson, James F., Wright, Alan F., Conley, Dalton C., Krueger, Robert F., Smith, George Davey, Hofman, Albert, Laibson, David I., Medland, Sarah E., Meyer, Michelle N., Johannesson, Magnus, Koellinger, Philipp D., Cesarini, David, Benjamin, Daniel J., Auton, Adam, Boardman, Jason D., Clark, David W., Conlin, Andrew, Dolan, Conor C., Fischbacher, Urs, Harris, Kathleen Mullan, Hasler, Gregor, Ikram, Mohammad A., Jain, Sonia, Kessler, Ronald C., Kooyman, Maarten, Mackillop, James, Männikkö, Minna, Morcillo-Suarez, Carlos, Mcqueen, Matthew B., Schmidt, Klaus M., Smart, Melissa C., Sutter, Matthias, White, Jon, Wit, Harriet de, Fehr, Ernst, Kumari, Meena, Laibson, David, Navarro, Arcadi, Palmer, Abraham A., Schunk, Daniel, Stein, Murray B., Svento, Rauli, Timmers, Paul R. H. J., Ursano, Robert J., Wagner, Gert G., Adult Psychiatry, APH - Mental Health, Psychiatry, Amsterdam Neuroscience - Complex Trait Genetics, Human genetics, Amsterdam Reproduction & Development (AR&D), APH - Digital Health, 23and Me Research Team, eQTLgen Consortium, International Cannabis Consortium, Economics, Biological Psychology, Complex Trait Genetics, APH - Methodology, Amsterdam Neuroscience - Mood, Anxiety, Psychosis, Stress & Sleep, APH - Health Behaviors & Chronic Diseases, APH - Personalized Medicine, Tinbergen Institute, 23andme Research Team, Psychiatric Genomics Consortium, Social Science Genetic Association Consortium, Linner Richard Karlsson, Biroli P, Kong Edward, Meddens Fleur W., Wedow Robbee, Fontana Mark Alan, Lebreton Mael, Tino Stephen P., Abdellaoui Abdel, Hammerschlag Anke R., Nivard Michel G., Okbay Aysu, Rietveld Cornelius A., Timshel Pascal N., Trzaskowski Maciej, de Vlaming Ronald, Zund Christian L., Bao Yanchun, Buzdugan Laura, Caplin Ann H., Chen Chia-Yen, Eibich Peter, Fontanillas Pierre, Gonzalez Juan R., Joshi Peter K., Karhunen Ville, Kleinman Aaron, Levin Remy Z., Lill Christina M., Meddens Gerardus A., Muntane Gerard, Sanchez-Roige Sandra, van Rooij Frank J., Taskesen Erdogan, Wu Yang, Zhang Futao, Agee Michelle, Alipanahi Babak, Bell Robert K., Bryc Katarzyna, Elson Sarah L., Furlotte Nicholas A., Huber Karen E., Litterman Nadia K., McCreight Jennifer C., McIntyre Matthew H., Mountain Joanna L., Northover Carrie A. M., Pitts Steven J., Sathirapongsasuti J. Fah, Sazonova Olga V., Shelton Janie F., Shringarpure Suyash, Tian Chao, Tung Joyce Y., Vacic Vladimir, Wilson Catherine H., Agbessi Mawusse, Ahsan Habibul, Alves Isabel, Andiappan Anand, Awadalla Philip, Battle Alexi, Beutner Frank, Bonder Marc Jan, Boomsma Dorret I., Christiansen Mark, Claringbould Annique, Deelen Patrick, Esko Tonu, Fave Marie-Julie, Franke Lude, Frayling Timothy, Gharib Sina A., Gibson Gregory, Heijmans Bastiaan, Hemani Gibran, Jansen Rick, Kahonen Mika, Kalnapenkis Anette, Kasela Silva, Kettunen Johanne, Kim Yungil, Kirsten Holger, Kovacs Peter, Krohn Knut, Kronberg-Guzman Jaanika, Kukushkina Viktorija, Kutalik Zoltan, Lee Bernett, Lehtimaki Terho, Loeffler Marku, Marigorta Urko M., Metspalu Andre, Milani Lili, Montgomery Grant W., Mueller-Nurasyid Martina, Nauck Matthia, Penninx Brenda, Perola Marku, Pervjakova Natalia, Pierce Brandon, Powell Joseph, Prokisch Holger, Psaty Bruce M., Raitakari Olli, Ring Susan, Ripatti Samuli, Rotzchke Olaf, Rueger Sina, Saha Ashi, Scholz Marku, Schramm Katharina, Seppala Ilkka, Stumvoll Michael, Sullivan Patrick, Hoen Peter-Bramt, Teumer Alexander, Thiery Joachim, Tong Lin, Tonjes Anke, van Dongen Jenny, van Meurs Joyce, Verlouw Joost, Visscher Peter M., Voelker Uwe, Vosa Urmo, Westra Harm-Jan, Yaghootkar Hanieh, Yang Jian, Zeng Biao, Lee James J., Pers Tune H., Turley Patrick, Chen Guo-Bo, Emilsson Valur, Oskarsson Sven, Pickrell Joseph K., Thom Kevin, Timshel Pascal, Ahluwalia Tarunveer S., Bacelis Jona, Baumbach Clemen, Bjornsdottir Gyda, Brandsma Johannes H., Concas Maria Pina, Derringer Jaime, Galesloot Tessel E., Girotto Giorgia, Gupta Richa, Hall Leanne M., Harris Sarah E., Hofer Edith, Horikoshi Momoko, Huffman Jennifer E., Kaasik Kadri, Kalafati Ioanna P., Kong Augustine, Lahti Jari, van der Lee Sven J., de Leeuw Christiaan, Lind Penelope A., Lindgren Karl-Oskar, Liu Tian, Mangino Massimo, Marten Jonathan, Mihailov Evelin, Miller Michael B., van der Most Peter J., Oldmeadow Christopher, Payton Antony, Peyrot Wouter J., Qian Yong, Rueedi Rico, Salvi Erika, Schmidt Boerge, Schraut Katharina E., Shi Jianxin, Smith Albert V., Poot Raymond A., St Pourcain Beate, Thorleifsson Gudmar, Verweij Niek, Vuckovic Dragana, Wellmann Juergen, Yang Jingyun, Zhao Wei, Zhu Zhihong, Alizadeh Behrooz Z., Amin Najaf, Bakshi Andrew, Baumeister Sebastian E., Biino Ginevra, Bonnelykke Klau, Boyle Patricia A., Campbell Harry, Cappuccio Francesco P., Davies Gail, De Neve Jan-Emmanuel, Deloukas Pano, Demuth Ilja, Ding Jun, Eisele Lewin, Eklund Niina, Evans David M., Faul Jessica D., Feitosa Mary F., Forstner Andreas J., Gandin Ilaria, Gunnarsson Bjarni, Halldorsson Bjarni V., Harris Tamara B., Heath Andrew C., Hocking Lynne J., Holliday Elizabeth G., Homuth Georg, Horan Michael A., Hottenga Jouke-Jan, de Jager Philip L., Jugessur Astanand, Kaakinen Marika A., Kanoni Stavroula, Keltigangas-Jarvinen Liisa, Kiemeney Lambertus A. L. M., Kolcic Ivana, Koskinen Seppo, Kraja Aldi T., Kroh Martin, Latvala Antti, Launer Lenore J., Lebreton Mael P., Levinson Douglas F., Lichtenstein Paul, Lichtner Peter, Liewald David C. M., Loukola Anu, Madden Pamela A., Magi Reedik, Maki-Opas Tomi, Marioni Riccardo E., Marques-Vidal Pedro, McMahon George, Meisinger Christa, Meitinger Thoma, Milaneschi Yusplitri, Myhre Ronny, Nelson Christopher P., Nyholt Dale R., Ollier William E. R., Palotie Aarno, Paternoster Lavinia, Pedersen Nancy L., Petrovic Katja E., Porteous David J., Raikkonen Katri, Ring Susan M., Robino Antonietta, Rostapshova Olga, Rudan Igor, Rustichini Aldo, Salomaa Veikko, Sanders Alan R., Sarin Antti-Pekka, Schmidt Helena, Scott Rodney J., Smith Blair H., Smith Jennifer A., Staessen Jan A., Steinhagen-Thiessen Elisabeth, Strauch Konstantin, Terracciano Antonio, Tobin Martin D., Ulivi Sheila, Vaccargiu Simona, Quaye Lydia, Venturini Cristina, Vinkhuyzen Anna A. E., Voelzke Henry, Vonk Judith M., Vozzi Diego, Waage Johanne, Ware Erin B., Willemsen Gonneke, Attia John R., Bennett David A., Berger Klau, Bertram Lar, Bisgaard Han, Borecki Ingrid B., Bultmann Ute, Chabris Christopher F., Cucca Francesco, Cusi Daniele, Deary J., Dedoussis George V., van Duijn Cornelia M., Eriksson Johan G., Franke Barbara, Gasparini Paolo, Gejman Pablo V., Gieger Christian, Grabe Hans-Joergen, Gratten Jacob, Gudnason Vilmundur, van der Harst Pim, Hayward Caroline, Hinds David A., Hoffmann Wolfgang, Hypponen Elina, Iacono William G., Jacobsson Bo, Jarvelin Marjo-Riitta, Jockel Karl-Heinz, Kaprio Jaakko, Kardia Sharon L. R., Lehrer Steven F., Magnusson Patrik K. E., Martin Nicholas G., McGue Matt, Pendleton Neil, Pirastu Nicola, Pirastu Mario, Polasek Ozren, Posthuma Danielle, Power Christine, Province Michael A., Samani Nilesh J., Schlessinger David, Schmidt Reinhold, Sorensen Thorkild I. A., Spector Tim D., Stefansson Kari, Thorsteinsdottir Unnur, Thurik A. Roy, Timpson Nicholas J., Tiemeier Henning, Uitterlinden Andre G., Vitart Veronique, Vollenweider Peter, Weir David R., Wilson James F., Wright Alan F., Conley Dalton C., Krueger Robert F., Smith George Davey, Laibson David I., Medland Sarah E., Johannesson Magnu, Koellinger Philipp D., Cesarini David, Benjamin Daniel J., Auton Adam, Boardman Jason D., Clark David W., Conlin Andrew, Dolan Conor C., Fischbacher Ur, Groenen Patrick J. F., Harris Kathleen Mullan, Hasler Gregor, Hofman Albert, Ikram Mohammad A., Jain Sonia, Karlsson Robert, Kessler Ronald C., Kooyman Maarten, MacKillop Jame, Mannikko Minna, Morcillo-Suarez Carlo, McQueen Matthew B., Schmidt Klaus M., Smart Melissa C., Sutter Matthia, White Jon, de Wit Harriet, Fehr Ernst, Kumari Meena, Laibson David, Meyer Michelle N., Navarro Arcadi, Palmer Abraham A., Schunk Daniel, Stein Murray B., Svento Rauli, Timmers Paul R. H. J., Ursano Robert J., Wagner Gert G., Beauchamp Jonathan P., Karlsson Linnér, Richard, Biroli, Pietro, Kong, Edward, Meddens, S. Fleur W., Wedow, Robbee, Fontana, Mark Alan, Lebreton, Maël, Tino, Stephen P., Abdellaoui, Abdel, Hammerschlag, Anke R., Nivard, Michel G., Okbay, Aysu, Rietveld, Cornelius A., Timshel, Pascal N., Trzaskowski, Maciej, Vlaming, Ronald de, Zünd, Christian L., Bao, Yanchun, Buzdugan, Laura, Caplin, Ann H., Chen, Chia-Yen, Eibich, Peter, Fontanillas, Pierre, Gonzalez, Juan R., Joshi, Peter K., Karhunen, Ville, Kleinman, Aaron, Levin, Remy Z., Lill, Christina M., Meddens, Gerardus A., Muntané, Gerard, Sanchez-Roige, Sandra, Rooij, Frank J. van, Taskesen, Erdogan, Wu, Yang, Zhang, Futao, Agee, Michelle, Alipanahi, Babak, Bell, Robert K., Bryc, Katarzyna, Elson, Sarah L., Furlotte, Nicholas A., Huber, Karen E., Litterman, Nadia K., Mccreight, Jennifer C., Mcintyre, Matthew H., Mountain, Joanna L., Northover, Carrie A. M., Pitts, Steven J., Sathirapongsasuti, J. Fah, Sazonova, Olga V., Shelton, Janie F., Shringarpure, Suyash, Tian, Chao, Tung, Joyce Y., Vacic, Vladimir, Wilson, Catherine H., Agbessi, Mawussé, Ahsan, Habibul, Alves, Isabel, Andiappan, Anand, Awadalla, Philip, Battle, Alexi, Beutner, Frank, Jan Bonder, Marc, Boomsma, Dorret I., Christiansen, Mark, Claringbould, Annique, Deelen, Patrick, Esko, Tõnu, Favé, Marie-Julie, Franke, Lude, Frayling, Timothy, Gharib, Sina A., Gibson, Gregory, Heijmans, Bastiaan, Hemani, Gibran, Jansen, Rick, Kähönen, Mika, Kalnapenkis, Anette, Kasela, Silva, Kettunen, Johanne, Kim, Yungil, Kirsten, Holger, Kovacs, Peter, Krohn, Knut, Kronberg-Guzman, Jaanika, Kukushkina, Viktorija, Kutalik, Zoltan, Lee, Bernett, Lehtimäki, Terho, Loeffler, Marku, Marigorta, Urko M., Metspalu, Andre, Milani, Lili, Montgomery, Grant W., Müller-Nurasyid, Martina, Nauck, Matthia, Penninx, Brenda, Perola, Marku, Pervjakova, Natalia, Pierce, Brandon, Powell, Joseph, Prokisch, Holger, Psaty, Bruce M., Raitakari, Olli, Ring, Susan, Ripatti, Samuli, Rotzchke, Olaf, Rüeger, Sina, Saha, Ashi, Scholz, Marku, Schramm, Katharina, Seppälä, Ilkka, Stumvoll, Michael, Sullivan, Patrick, Hoen, Peter-Bram t, Teumer, Alexander, Thiery, Joachim, Tong, Lin, Tönjes, Anke, Dongen, Jenny van, Meurs, Joyce van, Verlouw, Joost, Visscher, Peter M., Völker, Uwe, Võsa, Urmo, Westra, Harm-Jan, Yaghootkar, Hanieh, Yang, Jian, Zeng, Biao, Beauchamp, Jonathan P., Lee, James J., Pers, Tune H., Turley, Patrick, Chen, Guo-Bo, Emilsson, Valur, Oskarsson, Sven, Pickrell, Joseph K., Thom, Kevin, Timshel, Pascal, de Vlaming, Ronald, Ahluwalia, Tarunveer S., Bacelis, Jona, Baumbach, Clemen, Bjornsdottir, Gyda, Brandsma, Johannes H., Concas, Maria Pina, Derringer, Jaime, Galesloot, Tessel E., Girotto, Giorgia, Gupta, Richa, Hall, Leanne M., Harris, Sarah E., Hofer, Edith, Horikoshi, Momoko, Huffman, Jennifer E., Kaasik, Kadri, Kalafati, Ioanna P., Karlsson, Robert, Kong, Augustine, Lahti, Jari, Lee, Sven J. van der, de Leeuw, Christiaan, Lind, Penelope A., Lindgren, Karl-Oskar, Liu, Tian, Mangino, Massimo, Marten, Jonathan, Mihailov, Evelin, Miller, Michael B., Most, Peter J. van der, Oldmeadow, Christopher, Payton, Antony, Peyrot, Wouter J., Qian, Yong, Rueedi, Rico, Salvi, Erika, Schmidt, Börge, Schraut, Katharina E., Shi, Jianxin, Smith, Albert V., Poot, Raymond A., Pourcain, Beate St, Thorleifsson, Gudmar, Verweij, Niek, Vuckovic, Dragana, Wellmann, Juergen, Yang, Jingyun, Zhao, Wei, Zhu, Zhihong, Alizadeh, Behrooz Z., Amin, Najaf, Bakshi, Andrew, Baumeister, Sebastian E., Biino, Ginevra, Bønnelykke, Klau, Boyle, Patricia A., Campbell, Harry, Cappuccio, Francesco P., Davies, Gail, De Neve, Jan-Emmanuel, Deloukas, Pano, Demuth, Ilja, Ding, Jun, Eisele, Lewin, Eklund, Niina, Evans, David M., Faul, Jessica D., Feitosa, Mary F., Forstner, Andreas J., Gandin, Ilaria, Gunnarsson, Bjarni, Halldórsson, Bjarni V., Harris, Tamara B., Heath, Andrew C., Hocking, Lynne J., Holliday, Elizabeth G., Homuth, Georg, Horan, Michael A., Hottenga, Jouke-Jan, de Jager, Philip L., Jugessur, Astanand, Kaakinen, Marika A., Kanoni, Stavroula, Keltigangas-Järvinen, Liisa, Kiemeney, Lambertus A. L. M., Kolcic, Ivana, Koskinen, Seppo, Kraja, Aldi T., Kroh, Martin, Latvala, Antti, Launer, Lenore J., Lebreton, Maël P., Levinson, Douglas F., Lichtenstein, Paul, Lichtner, Peter, Liewald, David C. M., Loukola, Anu, Madden, Pamela A., Mägi, Reedik, Mäki-Opas, Tomi, Marioni, Riccardo E., Marques-Vidal, Pedro, Mcmahon, George, Meisinger, Christa, Meitinger, Thoma, Milaneschi, Yusplitri, Myhre, Ronny, Nelson, Christopher P., Nyholt, Dale R., Ollier, William E. R., Palotie, Aarno, Paternoster, Lavinia, Pedersen, Nancy L., Petrovic, Katja E., Porteous, David J., Räikkönen, Katri, Ring, Susan M., Robino, Antonietta, Rostapshova, Olga, Rudan, Igor, Rustichini, Aldo, Salomaa, Veikko, Sanders, Alan R., Sarin, Antti-Pekka, Schmidt, Helena, Scott, Rodney J., Smith, Blair H., Smith, Jennifer A., Staessen, Jan A., Steinhagen-Thiessen, Elisabeth, Strauch, Konstantin, Terracciano, Antonio, Tobin, Martin D., Ulivi, Sheila, Vaccargiu, Simona, Quaye, Lydia, Venturini, Cristina, Vinkhuyzen, Anna A. E., Völzke, Henry, Vonk, Judith M., Vozzi, Diego, Waage, Johanne, Ware, Erin B., Willemsen, Gonneke, Attia, John R., Bennett, David A., Berger, Klau, Bertram, Lar, Bisgaard, Han, Borecki, Ingrid B, Bültmann, Ute, Chabris, Christopher F., Cucca, Francesco, Cusi, Daniele, Deary, Ian J., Dedoussis, George V., Duijn, Cornelia M. van, Eriksson, Johan G., Franke, Barbara, Gasparini, Paolo, Gejman, Pablo V., Gieger, Christian, Grabe, Hans-Jörgen, Gratten, Jacob, Groenen, Patrick J. F., Gudnason, Vilmundur, Harst, Pim van der, Hayward, Caroline, Hinds, David A., Hoffmann, Wolfgang, Hyppönen, Elina, Iacono, William G., Jacobsson, Bo, Järvelin, Marjo-Riitta, Jöckel, Karl-Heinz, Kaprio, Jaakko, Kardia, Sharon L. R., Lehrer, Steven F., Magnusson, Patrik K. E., Martin, Nicholas G., Mcgue, Matt, Pendleton, Neil, Pirastu, Nicola, Pirastu, Mario, Polasek, Ozren, Posthuma, Danielle, Power, Christine, Province, Michael A., Samani, Nilesh J., Schlessinger, David, Schmidt, Reinhold, Sørensen, Thorkild I. A., Spector, Tim D., Stefansson, Kari, Thorsteinsdottir, Unnur, Thurik, A. Roy, Timpson, Nicholas J., Tiemeier, Henning, Uitterlinden, André G., Vitart, Veronique, Vollenweider, Peter, Weir, David R., Wilson, James F., Wright, Alan F., Conley, Dalton C., Krueger, Robert F., Smith, George Davey, Hofman, Albert, Laibson, David I., Medland, Sarah E., Meyer, Michelle N., Johannesson, Magnu, Koellinger, Philipp D., Cesarini, David, Benjamin, Daniel J., Auton, Adam, Boardman, Jason D., Clark, David W., Conlin, Andrew, Dolan, Conor C., Fischbacher, Ur, Harris, Kathleen Mullan, Hasler, Gregor, Ikram, Mohammad A., Jain, Sonia, Kessler, Ronald C., Kooyman, Maarten, Mackillop, Jame, Männikkö, Minna, Morcillo-Suarez, Carlo, Mcqueen, Matthew B., Schmidt, Klaus M., Smart, Melissa C., Sutter, Matthia, White, Jon, Wit, Harriet de, Fehr, Ernst, Kumari, Meena, Laibson, David, Navarro, Arcadi, Palmer, Abraham A., Schunk, Daniel, Stein, Murray B., Svento, Rauli, Timmers, Paul R. H. J., Ursano, Robert J., Wagner, Gert G., Applied Economics, Hematology, Epidemiology, Urology, Department of Marketing Management, Internal Medicine, Medical Informatics, Cell biology, Erasmus MC other, Molecular Genetics, Econometrics, Child and Adolescent Psychiatry / Psychology, Radiology & Nuclear Medicine, Epidemiologie, RS: CARIM - R3.02 - Hypertension and target organ damage, RS: Carim - V02 Hypertension and target organ damage, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Groningen Research Institute for Asthma and COPD (GRIAC), Public Health Research (PHR), Cardiovascular Centre (CVC), Life Course Epidemiology (LCE), Stem Cell Aging Leukemia and Lymphoma (SALL), Real World Studies in PharmacoEpidemiology, -Genetics, -Economics and -Therapy (PEGET), Linnér, Richard Karlsson, Biroli , Pietro, Meddens, S Fleur W, and Beauchamp, Jonathan P
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Netherlands Twin Register (NTR) ,Male ,LD ,Medizin ,Poison control ,Genome-wide association study ,DETERMINANTS ,Cathie Marsh Institute ,PREFERENCES ,Q1 ,Genome-wide association studies ,HV ,0302 clinical medicine ,Genetics, Behavioral/methods ,GWAS ,Genetics & Heredity ,Genetics ,0303 health sciences ,PERSONALITY ,Genetic Predisposition to Disease/genetics ,Behavior/physiology ,Polymorphism, Single Nucleotide/genetics ,3. Good health ,Manchester Institute for Collaborative Research on Ageing ,Meta-analysis ,Urological cancers Radboud Institute for Health Sciences [Radboudumc 15] ,Genetic Loci/genetics ,Behavioural genetics ,Genome-Wide Association Study/methods ,Medical genetics ,Female ,Life Sciences & Biomedicine ,Single Nucleotide/genetics ,medicine.medical_specialty ,ResearchInstitutes_Networks_Beacons/MICRA ,Genotype ,Single-nucleotide polymorphism ,Genetics, Behavioral ,Biology ,Polymorphism, Single Nucleotide ,Article ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,SDG 3 - Good Health and Well-being ,ddc:330 ,medicine ,GENDER-DIFFERENCES ,SNP ,Humans ,Genetic Predisposition to Disease ,Polymorphism ,behavioural genetics ,Behavioral/methods ,030304 developmental biology ,Genetic association ,Behavior ,Science & Technology ,Neurodevelopmental disorders Donders Center for Medical Neuroscience [Radboudumc 7] ,Data Science ,Case-control study ,risk tolerance, GWAS, genetic architechture ,Genetic Loci ,Case-Control Studies ,genome-wide association studies ,ResearchInstitutes_Networks_Beacons/cathie_marsh_institute ,Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] ,030217 neurology & neurosurgery ,Genome-Wide Association Study - Abstract
Humans vary substantially in their willingness to take risks. In a combined sample of over 1 million individuals, we conducted genome-wide association studies (GWAS) of general risk tolerance, adventurousness, and risky behaviors in the driving, drinking, smoking, and sexual domains. Across all GWAS, we identified hundreds of associated loci, including 99 loci associated with general risk tolerance. We report evidence of substantial shared genetic influences across risk tolerance and the risky behaviors: 46 of the 99 general risk tolerance loci contain a lead SNP for at least one of our other GWAS, and general risk tolerance is genetically correlated ([Formula: see text] ~ 0.25 to 0.50) with a range of risky behaviors. Bioinformatics analyses imply that genes near SNPs associated with general risk tolerance are highly expressed in brain tissues and point to a role for glutamatergic and GABAergic neurotransmission. We found no evidence of enrichment for genes previously hypothesized to relate to risk tolerance. This research was carried out under the auspices of the Social Science Genetic Association Consortium. The research was also conducted using the UK Biobank Resource under application number 11425. The study was supported by funding from the Ragnar Söderberg Foundation (E9/11 and E42/15); the Swedish Research Council (421-2013-1061); the Jan Wallander and Tom Hedelius Foundation; an ERC Consolidator Grant to Philipp Koellinger (647648 EdGe); the Pershing Square Fund of the Foundations of Human Behavior; the Open Philanthropy Project; the National Institute on Aging, National Institutes of Health through grants P01-AG005842, P01-AG005842-20S2, P30-AG012810, and T32-AG000186-23 to the National Bureau of Economic Research and R01-AG042568-02 to the University of Southern California; the government of Canada through Genome Canada and the Ontario Genomics Institute (OGI-152); and the Social Sciences and Humanities Research Council of Canada. We thank the International Cannabis Consortium, the eQTLgen Consortium, and the Psychiatric Genomics Consortium for sharing summary statistics from the GWAS of lifetime cannabis use, eQTL summary statistics, and summary statistics from the GWAS of ADHD, respectively. A full list of acknowledgments is provided in the Supplementary Note.
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- 2019
49. Hospital discharges in urban sanitation systems: Long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome
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Margaux Gaschet, José Lourenço, Laure Wiest, Elena Buelow, Christophe Dagot, Marie-Cécile Ploy, Sean Kennedy, Andreu Rico, Kennedy, Sean, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), IMDEA Water Institute, Universidad de Alcalá - University of Alcalá (UAH), University of Oxford, Pôle Biomics (C2RT), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris] (IP)-Institut Pasteur [Paris] (IP), TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), E. Buelow has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement RESOLVE 707999-standard EF., A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University (IJCI-2017-33,465)., J. Lourenço is supported by a Lectureship from the Department of Zoology, University of Oxford., 16S rRNA sequence Data collection and analyses performed by the IBEST Genomics Resources Core at the University of Idaho were supported in part by NIH COBRE grant P30GM103324., The authors thank the SIPIBEL Consortium and the SIPIBEL field observatory on the hospital’s effluents and urban wastewater treatment plants for displaying data and measurements., University of Oxford [Oxford], Institut Pasteur [Paris]-Institut Pasteur [Paris], Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
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Sanitation ,010501 environmental sciences ,Wastewater ,Antimicrobial resistance ,01 natural sciences ,AMR ,lcsh:Environmental technology. Sanitary engineering ,HWW ,Waste Management and Disposal ,Water Science and Technology ,0303 health sciences ,WW signatures ,Full Paper ,Ecological Modeling ,Microbiota ,ARG ,Resistome (ARGs and MGEs) ,ARB ,WW ,Pollution ,6. Clean water ,WW, Wastewater ,Hospital Wastewater ,Urban Wastewater ,GE Environmental Sciences ,Exposome ,Biology ,lcsh:TD1-1066 ,03 medical and health sciences ,Hospital and urban wastewater eco-exposome ,Environmental health ,Antibiotic-Resistant Gene ,ARB, Antibiotic-Resistant Bacteria ,Mobile Genetic Element ,030304 developmental biology ,0105 earth and related environmental sciences ,Hospital and urban wastewater ,HWW, Hospital Wastewater ,MGE, Mobile Genetic Element ,MGE ,UWW ,Antibiotic-Resistant Bacteria ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,AMR, Antimicrobial Resistance ,Resistome ,[SDE.BE] Environmental Sciences/Biodiversity and Ecology ,Long term monitoring ,UWW, Urban Wastewater ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,ARG, Antibiotic-Resistant Gene - Abstract
Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW., Graphical abstract Image 1, Highlights • Hospital and urban wastewater resistome and microbiota display specific signatures stable over time. • Hospital and urban wastewater have a distinct eco-exposome that is associated with their respective resistome and microbiota. • Mixing hospital with urban wastewater hides the " hospital signature” due to the dilution rate.
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- 2020
50. Considerations and consequences of allowing DNA sequence data as types of fungal taxa
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Harry Andersson, Annemieke Verbeken, László Nagy, E. S. Popov, L. B. Kalinina, Robert W. Barreto, Philippe Clerc, Alice Cruz Lima da Gerlach, Martin Grube, Xingzhong Liu, Jan Holec, Leticia Pacheco, Ursula Eberhardt, Blanca Imelda Arguello Sosa, Sigvard Svensson, Dania García Sánchez, Dmitry Ageev, Julia Pawłowska, Dennis E. Desjardin, Sara R. Noumeur, James C. Lendemer, Martin Kukwa, Viktor Papp, Isabel Salcedo, Maria Martha Dios, Richard W. Kerrigan, Reinhard Agerer, Jean-Michel Bellanger, Curtis R. Björk, Uwe Braun, François Valade, Víctor J. Rico, Ondřej Koukol, Ingvar Kärnefelt, Barbara Schulz, Attila Koszka, Gro Gulden, E. F. Malysheva, P. Brandon Matheny, Anton Shiryaev, Gerardo Mata, Mehdi Mehrabi, Taiga Kasuya, Tor Tønsberg, Ivana Kušan, Sergey Volobuev, Hans-Otto Baral, Esteri Ohenoja, Martin Kirchmair, Holger Thüs, Marian Jagers, Tuomo Niemelä, Begoña Aguirre-Hudson, J. Jennifer Luangsa-ard, Måns Svensson, Geir Mathiassen, Anna Rosling, Roy Watling, Meiriele da Silva, Eske De Crop, Ursula Peintner, Claudio Angelini, Mascha Hoffmeister, Vincent Demoulin, Miguel Ángel Ribes Ripoll, Paul Diederich, Takayuki Aoki, Nicolás Niveiro, Jiří Kout, Asunción Morte, Damien Ertz, Peter R. Johnston, Sergio P. Gorjón, Huzefa A. Raja, Machiel E. Noordeloos, Stellan Sunhede, László Lőkös, Cécile Gueidan, Gérald Gruhn, Bart Buyck, Roy E. Halling, Thomas Læssøe, Neven Matočec, Dan Mahoney, David Boertmann, Carlos G. Boluda, Vera Evenson, Ferenc Pál-Fám, Martin Westberg, Katriina Bendiksen, Jukka Vauras, Jacques Fournier, Martina Réblová, Gabriel Moreno, Yuri K. Novozhilov, Aída M. Vasco-Palacios, Leif Tibell, Deborah Jean Lodge, Miquel À. Pérez-De-Gregorio Capella, Rafael F. Castañeda-Ruiz, Olinto Liparini Pereira, Karl-Henrik Larsson, Michael Loizides, Edit Farkas, Mika Bendiksby, Tanja Böhning, Kadri Pärtel, Lucia Muggia, Brigitte Capoen, Raphaël Herve, Paul S. Dyer, Alberto Altés García, João Luís Baptista-Ferreira, Bella Grishkan, Paul Pirot, Karl Soop, Anna Bérešová-Guttová, Donald H. Pfister, A. Martyn Ainsworth, Uwe Lindemann, Alain Favre, Elisandro Ricardo Drechsler-Santos, André De Kesel, Mónica A.G. Otálora, Klaus Høiland, Ellen Larsson, Jens H. Petersen, Meike Piepenbring, Florent Boittin, James K. Mitchell, Zdeněk Palice, Franck Richard, Masoomeh Ghobad-Nejhad, Nils Hallenberg, Henry J. Beker, Gilles Corriol, Ronald H. Petersen, Melissa Palacio, Ana Esperanza Franco Molano, Mikael Jeppson, Gerardo Lucio Robledo, Egil Bendiksen, V. M. Kotkova, Håkon Holien, Marjo Dam, Pier Luigi Nimis, Yasmina Marin-Felix, Fernando Esteve-Raventós, Ave Suija, André Aptroot, Frank Dämmrich, Mitko Karadelev, Karen W. Hughes, Gladstone Alves da Silva, Emanuele Campo, Reinhard Berndt, Alona Yu. Biketova, Anders Nordin, Juan Manuel Velasco Santos, Josef Hafellner, Marco Thines, Bálint Dima, Grit Walther, Rodham E. Tulloss, Michael J. Richardson, Thomas W. Kuyper, Vladimír Kunca, Ann Bell, Adrien Taudière, Marc Stadler, Tania Raymundo, Per Vetlesen, Guillermo Muñoz González, Seppo Huhtinen, Irmgard Greilhuber, Øyvind Weholt, María Prieto Álvaro, Teun Boekhout, Dagmar Triebel, Mikhail P. Zhurbenko, Elena Voronina, Zdenko Tkalčec, Christian Lechat, Krzysztof Świerkosz, Joaquina María García-Martín, Johannes Z. Groenewald, Rubén Martínez-Gil, Pierre-Arthur Moreau, Evi Weber, Jan Borovička, Anna G. Fedosova, A Fraiture, Ewald Langer, Olga Morozova, Günter Saar, Carlos Lado, Vicent Calatayud, Juan Carlos Zamora, Ibai Olariaga, Francesco Bellù, Paolo Franchi, AnnaElise Jansen, Simón Fos, Matthias Lutz, Veera Tuovinen, István Nagy, Boris Assyov, J. Vladimir Sandoval-Sierra, Andrei Tsurykau, Alfredo Vizzini, Ivona Kautmanová, Mario Filippa, Beatrice Senn-Irlet, Sigisfredo Garnica, Josiane Santana Monteiro, Luis A. Parra, Svengunnar Ryman, Alan M. Fryday, Stip Helleman, Pedro W. Crous, Ruben De Lange, Alexander Ordynets, Giuliana Furci, Guilhermina Marques, Håkan Lindström, Joost A. Stalpers, Luis Quijada, Carlos A. Salvador Montoya, Marina Temina, Ruvishika S. Jayawardena, Miguel Ulloa Sosa, Joseph F. Ammirati, Heikki Kotiranta, Andreas Frisch, Martin Kříž, Teuvo Ahti, Tommy Knutsson, Tatyana Yu. Svetasheva, Luis Rubio Casas, Maria Alice Neves, Arne Thell, Soili Stenroos, Lajos Benedek, Sten Svantesson, Tine Grebenc, Patrícia Oliveira Fiuza, Tor Erik Brandrud, Flávia Rodrigues Barbosa, Annarosa Bernicchia, T. K. Arun Kumar, Massimo Candusso, Menno W. Boomsluiter, Wolfgang von Brackel, Petr Zehnálek, Hana Ševčíková, Toby Spribille, Vit Hubka, Trond Schumacher, Olivier Raspé, Tatiana Baptista Gibertoni, Esteve Llop, Åsa Kruys, Christoffer Bugge Harder, Klaus Siepe, Arne Aronsen, Andrew N. Miller, Laura Noemí Levin, Edgardo Albertó, Israel Pérez-Vargas, Hermann Voglmayr, Genevieve Gates, Bárbara De Madrignac Bonzi, Pradeep K. Divakar, Franz Berger, Natalia A. Ramírez, Per M. Jørgensen, Roland Moberg, Guy Marson, Gábor M. Kovács, Gérard Trichies, Sergio M. Salcedo Martínez, Juan Pablo Esquivel, Lynn Delgat, Juan de Dios Reyes García, Heidi Tamm, Vera Malysheva, Jan-Olof Tedebrand, Thomas Stjernegaard Jeppesen, Nico Dam, Régis Courtecuisse, Ireneia Melo, Pablo P. Daniëls, Péter Finy, Pamela Rodriguez-Flakus, Brian A. Perry, Brian Douglas, Ana M. Millanes Romero, Hans Josef Schroers, Pieter P. G. van den Boom, Slavomír Adamčík, Serena Lee, Marek Halama, Carlos Urcelay, Margarita Hernández-Restrepo, Philippe Callac, Oleg N. Shchepin, Vladimír Antonín, Gintaras Kantvilas, Else C. Vellinga, Ditte Bandini, Gernot Friebes, Roland Kirschner, Dániel G. Knapp, Boris Ivančević, Orlando Fabian Popoff, Clovis Douanla-Meli, Marcin Piątek, Alica Košuthová, Yury A. Rebriev, Helmut Mayrhofer, Alain Gardiennet, Karen Hansen, Kerry Knudsen, Otto Miettinen, Raquel Pino-Bodas, Shaun R. Pennycook, Beatriz Ortiz-Santana, Tatiana Bulyonkova, Jie Chen, Thomas Edison E. dela Cruz, Miroslav Kolařík, Witoon Purahong, Nicolas Van Vooren, Irwin M. Brodo, Esteban Benjamin Sir, Katerina Rusevska, Gerhard Rambold, Christian Printzen, Tim Baroni, Gary Laursen, Csaba Locsmándi, Javier Angel Etayo Salazar, Cristina Rodriguez-Caycedo, Irja Saar, Nadezhda V. Psurtseva, Takashi Shirouzu, Chayanard Phukhamsakda, Adam Flakus, Viacheslav Spirin, Sergi Santamaria, Matteo Garbelotto, Alan Orange, Mats Wedin, Andrew S. Methven, Huang Zhang, Guillaume Eyssartier, Michel Hairaud, Hatira Taskin, Luís Fernando Pascholati Gusmão, Carlos Manuel Pérez del Amo, Martin Bemmann, Ana Rosa Burgaz, Linas Kudzma, Didier Argaud, M. Catherine Aime, Alain Henriot, Walter M. Jaklitsch, Raúl Tena Lahoz, Violeta Atienza, Jorinde Nuytinck, Anna Kiyashko, Patinjareveettil Manimohan, József Geml, Cathy L. Cripps, Viktor Kučera, Francisco Kuhar, Kanad Das, Michael A. Castellano, Giovanni Consiglio, Ana Crespo, Armin Mešić, Leena Myllys, Einar Timdal, Ricardo Valenzuela Garza, Harold H. Burdsall, Enrico Bizio, Mohammad Sohrabi, Eugene Yurchenko, Linda Davies, Jacob Heilmann-Clausen, Patrice Lainé, Matteo Domenico Carbone, Aurelia Paz, Joaquim Carbó, Henning Knudsen, Thorsten Lumbsch, Caroline Hobart, Göran Thor, Bita Asgari, Matthias Lüderitz, Sanja Tibell, Ulf Arup, Geert Schmidt-Stohn, Urmas Kõljalg, Stefan Ekman, Regulo Carlos Llarena Hernandez, László Albert, Santiago Sánchez-Ramírez, Sergio Pérez-Ortega, Anna Ronikier, Isaac Garrido Benavent, Ricardo Galán Márquez, Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Yeast Research, Westerdijk Fungal Biodiversity Institute - Evolutionary Phytopathology, Uppsala University, National Central University, Universidad Rey Juan Carlos [Madrid] (URJC), Avenida Padre Claret 7, Partenaires INRAE, Naturalis Biodiversity Center [Leiden], Evolutionary Biology Centre, Slovak Academy of Sciences (SAS), Finnish Museum of Natural History (LUOMUS), University of Helsinki, Purdue University, Royal Botanic Gardens, Hungarian Mycological Society, Universidad Nacional de San Martin (UNSAM), Universidad de Alcalá - University of Alcalá (UAH), SIGNATEC Ltd., Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), University of Washington [Seattle], Jardín Botánico Nacional Dr. Rafael Ma. Moscoso, Moravian Museum, National Agriculture and Food Research Organization (NARO), ABL Herbarium, Auteur indépendant, Instituto Tecnológico de Ciudad Victoria, Torødveien 54, Lund University [Lund], Agricultural Research,Education and Extension Organization (ARREO), Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (BAS), Universitat Politècnica de València (UPV), Panoramastr 47, Universidade de Lisboa (ULISBOA), Blaihofstr. 42, State University of New York (SUNY), Universidade Federal de Vicosa (UFV), Royal Holloway, University of London, Meise Botanic Garden, 45 Gurney Road, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Naturmusem of Bolzano, Kleingemünderstraße 111, Norwegian University of Science and Technology (NTNU), Norwegian Institute for Nature Research (NINA), University of Oslo (UiO), Szent István University, University of Salzburg, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Via A. Guidotti 39, Institute of Biochemistry, Società Veneziana di Scienze Naturali, University of British Columbia (UBC), Westerdijk Fungal Biodiversity Insitute [Utrecht] (WI), Royal Netherlands Academy of Arts and Sciences (KNAW), Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam [Amsterdam] (UvA), Aarhus University [Aarhus], Christian-Albrechts University of Kiel, Conservatoire et Jardin Botaniques de Genève, T.v.Lohuizenstraat 34, Institute of Geology, Czech Academy of Sciences [Prague] (CAS), Martin-Luther-University Halle-Wittenberg, Canadian Museum of Nature, Russian Academy of Sciences [Moscow] (RAS), Fungal & Decay Diagnostics, Muséum national d'Histoire naturelle (MNHN), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Fundación CEAM, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Associazione Micologica Bresadola - Emilia Romagna, Via Ottone Primo 90, Via Don Luigi Sturzo, Instituto de Investigaciones Fundamentales en Agricultura Tropical 'Alejandro de Humboldt', United States Department of Agriculture (USDA), Mae Fah Luang University [Thaïlande] (MFU), Via C. Ronzani 61, Conservatoire Botanique National de Midi-Pyrénées (CBNMP), Université de Lille, Montana State University (MSU), Universidade Federal de Pernambuco [Recife] (UFPE), Hooischelf 13, Bavarian Natural History Collections, Botanical Survey of India, Centre for Environmental Policy, Imperial College London, Universiteit Gent = Ghent University [Belgium] (UGENT), Carlos Zamora, Juan, Svensson, Mån, Kirschner, Roland, Olariaga, Ibai, Ryman, Svengunnar, Alberto Parra, Lui, Geml, József, Rosling, Anna, Adamčík, Slavomír, Ahti, Teuvo, Catherine Aime, M., Martyn Ainsworth, A., Albert, László, Albertó, Edgardo, Altés García, Alberto, Ageev, Dmitry, Agerer, Reinhard, Aguirre-Hudson, Begoña, Ammirati, Joe, Andersson, Harry, Angelini, Claudio, Antonín, Vladimír, Aoki, Takayuki, Aptroot, André, Argaud, Didier, Imelda Arguello Sosa, Blanca, Aronsen, Arne, Arup, Ulf, Asgari, Bita, Assyov, Bori, Atienza, Violeta, Bandini, Ditte, Luís Baptista-Ferreira, João, Baral, Hans-Otto, Baroni, Tim, Weingart Barreto, Robert, Beker, Henry, Bell, Ann, Bellanger, Jean-Michel, Bellù, Francesco, Bemmann, Martin, Bendiksby, Mika, Bendiksen, Egil, Bendiksen, Katriina, Benedek, Lajo, Bérešová-Guttová, Anna, Berger, Franz, Berndt, Reinhard, Bernicchia, Annarosa, Biketova, Alona Yu., Bizio, Enrico, Bjork, Curti, Boekhout, Teun, Boertmann, David, Böhning, Tanja, Boittin, Florent, Boluda, Carlos G., Boomsluiter, Menno W., Borovička, Jan, Erik Brandrud, Tor, Braun, Uwe, Brodo, Irwin, Bulyonkova, Tatiana, H. Burdsall Jr., Harold, Buyck, Bart, Rosa Burgaz, Ana, Calatayud, Vicent, Callac, Philippe, Campo, Emanuele, Candusso, Massimo, Capoen, Brigitte, Carbó, Joaquim, Carbone, Matteo, Castañeda-Ruiz, Rafael F., Castellano, Michael A., Chen, Jie, Clerc, Philippe, Consiglio, Giovanni, Corriol, Gille, Courtecuisse, Régi, Crespo, Ana, Cripps, Cathy, Crous, Pedro W., Alves da Silva, Gladstone, da Silva, Meiriele, Dam, Marjo, Dam, Nico, Dämmrich, Frank, Das, Kanad, Davies, Linda, De Crop, Eske, De Kesel, Andre, De Lange, Ruben, De Madrignac Bonzi, Bárbara, dela Cruz, Thomas Edison E., Delgat, Lynn, Demoulin, Vincent, Desjardin, Dennis E., Diederich, Paul, Dima, Bálint, Martha Dios, Maria, Kumar Divakar, Pradeep, Douanla-Meli, Clovi, Douglas, Brian, Ricardo Drechsler-Santos, Elisandro, Dyer, Paul S., Eberhardt, Ursula, Ertz, Damien, Esteve-Raventós, Fernando, Angel Etayo Salazar, Javier, Evenson, Vera, Eyssartier, Guillaume, Farkas, Edit, Favre, Alain, Fedosova, Anna G., Filippa, Mario, Finy, Péter, Flakus, Adam, Fos, Simón, Fournier, Jacque, Fraiture, André, Franchi, Paolo, Esperanza Franco Molano, Ana, Friebes, Gernot, Frisch, Andrea, Fryday, Alan, Furci, Giuliana, Galán Márquez, Ricardo, Garbelotto, Matteo, María García-Martín, Joaquina, García Otálora, Mónica A., García Sánchez, Dania, Gardiennet, Alain, Garnica, Sigisfredo, Garrido Benavent, Isaac, Gates, Genevieve, da Cruz Lima Gerlach, Alice, Ghobad-Nejhad, Masoomeh, Gibertoni, Tatiana B., Grebenc, Tine, Greilhuber, Irmgard, Grishkan, Bella, Groenewald, Johannes Z., Grube, Martin, Gruhn, Gérald, Gueidan, Cécile, Gulden, Gro, FP Gusmão, Lui, Hafellner, Josef, Hairaud, Michel, Halama, Marek, Hallenberg, Nil, Halling, Roy E., Hansen, Karen, Bugge Harder, Christoffer, Heilmann-Clausen, Jacob, Helleman, Stip, Henriot, Alain, Hernandez-Restrepo, Margarita, Herve, Raphaël, Hobart, Caroline, Hoffmeister, Mascha, Høiland, Klau, Holec, Jan, Holien, Håkon, Hughes, Karen, Hubka, Vit, Huhtinen, Seppo, Ivančević, Bori, Jagers, Marian, Jaklitsch, Walter, Jansen, Annaelise, Jayawardena, Ruvishika S., Stjernegaard Jeppesen, Thoma, Jeppson, Mikael, Johnston, Peter, Magnus Jørgensen, Per, Kärnefelt, Ingvar, Kalinina, Liudmila B., Kantvilas, Gintara, Karadelev, Mitko, Kasuya, Taiga, Kautmanová, Ivona, Kerrigan, Richard W., Kirchmair, Martin, Kiyashko, Anna, Knapp, Dániel G., Knudsen, Henning, Knudsen, Kerry, Knutsson, Tommy, Kolařík, Miroslav, Kõljalg, Urma, Košuthová, Alica, Koszka, Attila, Kotiranta, Heikki, Kotkova, Vera, Koukol, Ondřej, Kout, Jiří, Kovács, Gábor M., Kříž, Martin, Kruys, Åsa, Kučera, Viktor, Kudzma, Lina, Kuhar, Francisco, Kukwa, Martin, Arun Kumar, T. K., Kunca, Vladimír, Kušan, Ivana, Kuyper, Thomas W., Lado, Carlo, Læssøe, Thoma, Lainé, Patrice, Langer, Ewald, Larsson, Ellen, Larsson, Karl-Henrik, Laursen, Gary, Lechat, Christian, Lee, Serena, Lendemer, James C., Levin, Laura, Lindemann, Uwe, Lindström, Håkan, Liu, Xingzhong, Carlos Llarena Hernandez, Regulo, Llop, Esteve, Locsmándi, Csaba, Jean Lodge, Deborah, Loizides, Michael, Lőkös, László, Luangsa-ard, Jennifer, Lüderitz, Matthia, Lumbsch, Thorsten, Lutz, Matthia, Mahoney, Dan, Malysheva, Ekaterina, Malysheva, Vera, Manimohan, Patinjareveettil, Marin-Felix, Yasmina, Marques, Guilhermina, Martínez-Gil, Rubén, Marson, Guy, Mata, Gerardo, Brandon Matheny, P., Harald Mathiassen, Geir, Matočec, Neven, Mayrhofer, Helmut, Mehrabi, Mehdi, Melo, Ireneia, Mešić, Armin, Methven, Andrew S., Miettinen, Otto, Millanes Romero, Ana M., Miller, Andrew N., Mitchell, James K., Moberg, Roland, Moreau, Pierre-Arthur, Moreno, Gabriel, Morozova, Olga, Morte, Asunción, Muggia, Lucia, Muñoz González, Guillermo, Myllys, Leena, Nagy, István, Nagy, László G., Alice Neves, Maria, Niemelä, Tuomo, Nimis, Pierluigi, Niveiro, Nicola, Noordeloos, Machiel E., Nordin, Ander, Raouia Noumeur, Sara, Novozhilov, Yuri, Nuytinck, Jorinde, Ohenoja, Esteri, Oliveira Fiuza, Patricia, Orange, Alan, Ordynets, Alexander, Ortiz-Santana, Beatriz, Pacheco, Leticia, Pál-Fám, Ferenc, Palacio, Melissa, Palice, Zdeněk, Papp, Viktor, Pärtel, Kadri, Pawlowska, Julia, Paz, Aurelia, Peintner, Ursula, Pennycook, Shaun, Liparini Pereira, Olinto, Pérez Daniëls, Pablo, Pérez-De-Gregorio Capella, Miquel À., Manuel Pérez del Amo, Carlo, Pérez Gorjón, Sergio, Pérez-Ortega, Sergio, Pérez-Vargas, Israel, Perry, Brian A., Petersen, Jens H., Petersen, Ronald H., Pfister, Donald H., Phukhamsakda, Chayanard, Piątek, Marcin, Piepenbring, Meike, Pino-Bodas, Raquel, Pablo Pinzón Esquivel, Juan, Pirot, Paul, Popov, Eugene S., Popoff, Orlando, Prieto Álvaro, María, Printzen, Christian, Psurtseva, Nadezhda, Purahong, Witoon, Quijada, Lui, Rambold, Gerhard, Ramírez, Natalia A., Raja, Huzefa, Raspé, Olivier, Raymundo, Tania, Réblová, Martina, Rebriev, Yury A., de Dios Reyes García, Juan, Ángel Ribes Ripoll, Miguel, Richard, Franck, Richardson, Mike J., Rico, Víctor J., Lucio Robledo, Gerardo, Rodrigues Barbosa, Flavia, Rodriguez-Caycedo, Cristina, Rodriguez-Flakus, Pamela, Ronikier, Anna, Rubio Casas, Lui, Rusevska, Katerina, Saar, Günter, Saar, Irja, Salcedo, Isabel, Salcedo Martínez, Sergio M., Salvador Montoya, Carlos A., Sánchez-Ramírez, Santiago, Vladimir Sandoval-Sierra, J., Santamaria, Sergi, Santana Monteiro, Josiane, Josef Schroers, Han, Schulz, Barbara, Schmidt-Stohn, Geert, Schumacher, Trond, Senn-Irlet, Beatrice, Ševčíková, Hana, Shchepin, Oleg, Shirouzu, Takashi, Shiryaev, Anton, Siepe, Klau, Sir, Esteban B., Sohrabi, Mohammad, Soop, Karl, Spirin, Viacheslav, Spribille, Toby, Stadler, Marc, Stalpers, Joost, Stenroos, Soili, Suija, Ave, Sunhede, Stellan, Svantesson, Sten, Svensson, Sigvard, Svetasheva, Tatyana Yu., Świerkosz, Krzysztof, Tamm, Heidi, Taskin, Hatira, Taudière, Adrien, Tedebrand, Jan-Olof, Tena Lahoz, Raúl, Temina, Marina, Thell, Arne, Thines, Marco, Thor, Göran, Thüs, Holger, Tibell, Leif, Tibell, Sanja, Timdal, Einar, Tkalčec, Zdenko, Tønsberg, Tor, Trichies, Gérard, Triebel, Dagmar, Tsurykau, Andrei, Tulloss, Rodham E., Tuovinen, Veera, Ulloa Sosa, Miguel, Urcelay, Carlo, Valade, Françoi, Valenzuela Garza, Ricardo, van den Boom, Pieter, Van Vooren, Nicola, Vasco-Palacios, Aida M., Vauras, Jukka, Manuel Velasco Santos, Juan, Vellinga, Else, Verbeken, Annemieke, Vetlesen, Per, Vizzini, Alfredo, Voglmayr, Hermann, Volobuev, Sergey, von Brackel, Wolfgang, Voronina, Elena, Walther, Grit, Watling, Roy, Weber, Evi, Wedin, Mat, Weholt, Øyvind, Westberg, Martin, Yurchenko, Eugene, Zehnálek, Petr, Zhang, Huang, Zhurbenko, Mikhail P., Ekman, Stefan, Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Purdue University [West Lafayette], Universidade de Lisboa = University of Lisbon (ULISBOA), Universidade Federal de Viçosa = Federal University of Viçosa (UFV), Meise Botanic Garden [Belgium] (Plantentuin), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Westerdijk Fungal Biodiversity Institute [Utrecht] (WI), Conservatoire et Jardin Botaniques de Genève (CJBG), Staatlichen Naturwissenschaftlichen Sammlungen Bayerns (SNSB), Universiteit Gent = Ghent University (UGENT), Finnish Museum of Natural History, Plant Biology, Tuula Niskanen / Principal Investigator, Botany, Doctoral Programme in Wildlife Biology, IMT Lille Douai, Institut Catholique Lille, Univ. Artois, IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - EA 4483, Naturalis Biodiversity Center, and Evolutionary and Population Biology (IBED, FNWI)
- Subjects
0106 biological sciences ,0301 basic medicine ,POSITIVE SELECTION ,Biologisk systematik ,VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 ,Speciation ,[SDV]Life Sciences [q-bio] ,IMC11 ,nomenclature ,speciation ,taxonomy ,typification ,voucherless fungi ,Biodiversity ,voucherless fung ,VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Systematisk botanikk: 493 ,Biological Systematics ,Matematikk og Naturvitenskap: 400::Basale biofag: 470 [VDP] ,01 natural sciences ,Voucherless fungi ,purl.org/becyt/ford/1 [https] ,Typification ,Environmental DNA ,CY3-LABELED OLIGONUCLEOTIDE PROBES ,Nomenclature ,ta119 ,GENE TREES ,1184 Genetics, developmental biology, physiology ,Soil Biology ,FRESH-WATER FUNGI ,PE&RC ,Agricultural and Biological Sciences (miscellaneous) ,INTERNAL TRANSCRIBED SPACER ,FUNGAL PHYLOGENY ,[SDE]Environmental Sciences ,Taxonomy (biology) ,RIBOSOMAL-RNA ,INTEGRATIVE TAXONOMY ,Biology ,VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 ,010603 evolutionary biology ,OPERATIONAL TAXONOMIC UNITS ,DNA sequencing ,Article ,SPECIES DELIMITATION ,03 medical and health sciences ,IMC11 nomenclature ,Internal transcribed spacer ,purl.org/becyt/ford/1.6 [https] ,Ecology, Evolution, Behavior and Systematics ,Bodembiologie ,Taxonomy ,VDP::Mathematics and natural science: 400::Zoology and botany: 480::Systematic botany: 493 ,Biology and Life Sciences ,IN-SITU HYBRIDIZATION ,CY3-LABELED ,Laboratorium voor Phytopathologie ,voucherless ,030104 developmental biology ,Taxon ,Evolutionary biology ,Laboratory of Phytopathology ,ta1181 ,BIODIVERSITY ,fungi ,OLIGONUCLEOTIDE PROBES - Abstract
Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to amend the International Code of Nomenclature for algae, fungi, and plants (ICN), allowing DNA sequences alone (of any region and extent) to serve as types of taxon names for voucherless fungi (mainly putative taxa from environmental DNA sequences), have been submitted to be voted on at the 11th International Mycological Congress (Puerto Rico, July 2018). We consider various genetic processes affecting the distribution of alleles among taxa and find that alleles may not consistently and uniquely represent the species within which they are contained. Should the proposals be accepted, the meaning of nomenclatural types would change in a fundamental way from physical objects as sources of data to the data themselves. Such changes are conducive to irreproducible science, the potential typification on artefactual data, and massive creation of names with low information content, ultimately causing nomenclatural instability and unnecessary work for future researchers that would stall future explorations of fungal diversity. We conclude that the acceptance of DNA sequences alone as types of names of taxa, under the terms used in the current proposals, is unnecessary and would not solve the problem of naming putative taxa known only from DNA sequences in a scientifically defensible way. As an alternative, we highlight the use of formulas for naming putative taxa (candidate taxa) that do not require any modification of the ICN. Publisher’s Note A first version of this text was prepared by the first eight authors and the last one, given here. The other listed co-authors in the article PDF support the content, and their actual contributions varied from only support to additions that substantially improved the content. The full details of all co-authors, with their affiliations, are included in Supplementary Table 1 after p.175 of the article for reasons of clarity and space. Slavomír Adamčík Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 845 23 Bratislava, Slovakia Teuvo Ahti Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland M. Catherine Aime Purdue University, 915 W. State St., West Lafayette, Indiana 47907, U.S.A. A. Martyn Ainsworth Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, United Kingdom László Albert Hungarian Mycological Society, 1087 Könyves Kálmán krt. 40, Budapest, Hungary Edgardo Albertó Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de San Martin-Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina Alberto Altés García Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, 28805 Alcalá de Henares, Madrid, Spain Dmitry Ageev SIGNATEC Ltd., 630090, Novosibirsk, Akademgorodok (Novosibirsk Scientific Center), Inzhenernaya str., 22, Russia Reinhard Agerer Ludwig-Maximilians-Universität München, Menzinger Str. 67, 80638 München, Germany Begona Aguirre-Hudson Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, United Kingdom Joe Ammirati University of Washington, Seattle, Washington 98195-1800, U.S.A. Harry Andersson Eichhahnweg 29a, 38108 Braunschweig, Germany Claudio Angelini Jardín Botánico Nacional Dr. Rafael Ma. Moscoso, Apartado 21-9, Santo Domingo, Dominican Republic Vladimír Antonín Moravian Museum, Zeny trh 6, 659 37 Brno, Czech Republic Takayuki Aoki Genetic Resources Center, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan André Aptroot ABL Herbarium, G.v.d.Veenstraat 107, 3762 XK Soest, The Netherlands Didier Argaud 40 rue du Justemont, 57290 Fameck, France Blanca Imelda Arguello Sosa Instituto Tecnológico de Ciudad Victoria, Tecnológico Nacional de México, Ciudad Victoria, Tamaulipas, Mexico Arne Aronsen Torødveien 54, 3135 Torød, Norway Ulf Arup Biological Museum, Lund University, Box 117, 221 00 Lund, Sweden Bita Asgari Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran Boris Assyov Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str., 1113 Sofia, Bulgaria Violeta Atienza Facultad de Ciencias Biológicas, Universitat de València, C/Dr Moliner 50, 46100, Burjasot, Valencia, Spain Ditte Bandini Panoramastr 47, 69257 Wiesenbach, Germany João Luís Baptista-Ferreira Instituto de Biossistemas e Ciências Integrativas, Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal Hans-Otto Baral Blaihofstr. 42, 72074 Tübingen, Germany Tim Baroni The State University of New York, 340 Bowers Hall, P.O. Box 2000, Cortland, New York 13045, U.S.A. Robert Weingart Barreto Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil Henry Beker (1) Royal Holloway, University of London, United Kingdom; (2) Botanic Garden Meise, Nieuwelaan 38, 1860 Meise, Belgium Ann Bell 45 Gurney Road, Lower Hutt, New Zealand Jean-Michel Bellanger CEFE UMR5175, CNRS, Université de Montpellier, Université Paul-Valéry Montpellier, EPHE, INSERM, 1919 Route de Mende, 34293 Montpellier Cédex 5, France Francesco Bellù Naturmusem of Bolzano, CP 104, 39100, Bolzano, Italy Martin Bemmann Kleingemünderstraße 111, 69118 Heidelberg, Germany Mika Bendiksby NTNU, University Museum, Norwegian University of Science and Technology, 7491 Trondheim, Norway Egil Bendiksen Norwegian Institute for Nature Research, Gaustadalleen 21, 0349 Oslo, Norway Katriina Bendiksen Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway Lajos Benedek Szent Istvan University, Hungary Anna Bérešová-Guttová Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 845 23 Bratislava, Slovakia Franz Berger University of Salzburg, Salzburg, Austria Reinhard Berndt Herbaria Z+ZT, ETH Zürich, CHN D37, Universitätstr. 16, 8092 Zürich, Switzerland Annarosa Bernicchia Via A. Guidotti 39, 40134 Bologna, Italy Alona Yu. Biketova Institute of Biochemistry, BRC-HAS, 6726 Szeged, Temesvari krt. 62, 6726 Szeged, Hungary Enrico Bizio Società Veneziana di Micologia, Società Veneziana di Scienze Naturali, Fontego dei Turchi, Santa Croce 1730, 30135 Venice, Italy Curtis Bjork UBC Herbarium, Beaty Biodiversity Museum, University of British Columbia, Canada Teun Boekhout (1) Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands; (2) Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands David Boertmann Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark Tanja Böhning AG Geobotanik Schleswig-Holstein & Hamburg, c/o University of Kiel, Olshausenstraße 75, 24098 Kiel, Germany Florent Boittin Ascomycete.org, 36 rue de la Garde, 69005 Lyon, France Carlos G. Boluda Conservatoire et Jardin botaniques de la Ville de Genève, 1292 Genève, Switzerland Menno W. Boomsluiter T.v.Lohuizenstraat 34, 8172xl, Vaassen, The Netherlands Jan Borovička Institute of Geology, Czech Academy of Sciences, Rozvojova 269, 165 00 Prague 6, Czech Republic Tor Erik Brandrud Norwegian Institute for Nature Research, Gaustadalleen 21, 0349 Oslo, Norway Uwe Braun Martin-Luther-Universität, Institut für Biologie, Bereich Geobotanik, und Botanischer Garten, Herbarium, Neuwerk 21, 06099 Halle, Germany Irwin Brodo Canadian Museum of Nature, 240 McLeod Street, Ottawa, Ontario, Canada Tatiana Bulyonkova A.P. Ershov Institute of Informatics Systems, Russian Academy of Sciences, Siberian Branch, 6 Acad. Lavrentjev pr., Novosibirsk 630090, Russia Harold H. Burdsall Jr. Fungal & Decay Diagnostics, LLC, 9350 Union Valley Road, Black Earth, Wisconsin 53515, U.S.A. Bart Buyck Muséum National d’Histoire Naturelle, CP 39, ISYEB, UMR 7205 CNRS MNHN UPMC EPHE, 12 rue Buffon, 75005 Paris, France Ana Rosa Burgaz Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, 28040 Madrid, Spain Vicent Calatayud Fundación CEAM, c/ Charles R. Darwin, 14, Parque Tecnológico, 46980 Paterna, Valencia, Spain Philippe Callac INRA, MycSA, CS 20032, 33882 Villenave d’Ornon, France Emanuele Campo Associazione Micologica Bresadola, Via Alessandro Volta 46, 38123 Trento, Italy Massimo Candusso Via Ottone Primo 90, 17021, Alassio, Savona, Italy Brigitte Capoen Queffioec, rue de Saint Gonval, 22710 Penvenan, France Joaquim Carbó Roser, 60, 17257 Torroella de Montgrí, Girona, Spain Matteo Carbone Via Don Luigi Sturzo 173 16148 Genova, Italy Rafael F. Castañeda-Ruiz Instituto de Investigaciones Fundamentales en Agricultura, Tropical ‘Alejandro de Humboldt’, OSDE, Grupo Agrícola, Calle 1 Esq. 2, Santiago de Las Vegas, C. Habana 17200, Cuba Michael A. Castellano USDA, Forest Service, Northern Research Station, Corvallis, Oregon 97330, U.S.A. Jie Chen Mae Fah Luang University, Chang Wat Chiang Rai 57100, Thailand Philippe Clerc Conservatoire et Jardin botaniques de la Ville de Genève, 1292 Genève, Switzerland Giovanni Consiglio Via C. Ronzani 61, 40033 Casalecchio Bologna, Italy Gilles Corriol National Botanical Conservatory for Pyrenees and Midi-Pyrénées Region of France and BBF Herbarium, Vallon de Salut. B.P. 315. 65203 Bagnères-de-Bigorre, France Régis Courtecuisse Université Lille, Fac. Pharma. Lille, EA4483 IMPECS, 59000 Lille, France Ana Crespo Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain Cathy Cripps Plant Sciences & Plant Pathology, 119 Plant Biosciences Building, Montana State University, Bozeman, Montana 59717, U.S.A. Pedro W. Crous Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands Gladstone Alves da Silva Universidade Federal de Pernambuco, Centro de Biociências, Avenida da Engenharia, S/N, Cidade Universitária, Recife, Pernambuco, Brazil Meiriele da Silva Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil Marjo Dam Hooischelf 13, 6581 SL Malden, The Netherlands Nico Dam Hooischelf 13, 6581 SL Malden, The Netherlands Frank Dämmrich The Bavarian Natural History Collections (SNSB Munich), Menzinger Strasse 71, 80638, München, Germany Kanad Das Botanical Survey of India, Cryptogamic Unit, P.O. Botanic Garden, Howrah 711103, W.B., India Linda Davies Centre for Environmental Policy, Imperial College London, SW7 2AZ, United Kingdom Eske De Crop Ghent University K.L. Ledeganckstraat 35, 9000 Ghent, Belgium Andre De Kesel Botanic Garden Meise, Nieuwelaan 38, 1860 Meise, Belgium Ruben De Lange Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium Bárbara De Madrignac Bonzi Instituto de Botánica del Nordeste, Universidad Nacional de Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas, Sargento Cabral 2131, CC 209, Corrientes Capital, Argentina Thomas Edison E. dela Cruz University of Santo Tomas, Espana 1008 Manila, Philippines Lynn Delgat Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium Vincent Demoulin Institut de Botanique, B.22, Université de Liège, 4000 Liège I, Belgium Dennis E. Desjardin HD Thiers Herbarium (SFSU), San Francisco State University, 1600 Holloway Ave, San Francisco, California 94132, U.S.A. Paul Diederich Musée national d’histoire naturelle, 25 rue Münster, 2160 Luxembourg, Luxembourg Bálint Dima (1) Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary; (2) Viikki Plant Science Centre, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland Maria Martha Dios Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Catamarca, Av Belgrano 300, 4700 San Fernando del Valle de Catamarca, Argentina Pradeep Kumar Divakar Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain Clovis Douanla-Meli Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for National and International Plant Health, Messeweg 11-12, 38104 Braunschweig, Germany Brian Douglas Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, United Kingdom Elisandro Ricardo Drechsler-Santos Universidade Federal de Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Trindade, Florianópolis, Santa Catarina CEP 88040-900, Brazil Paul S. Dyer School of Life Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom Ursula Eberhardt Abt. Botanik, Staatliches Museum für Naturkunde Stuttgart, Rosenstein 1, 70191 Stuttgart, Germany Damien Ertz Botanic Garden Meise, Nieuwelaan 38, 1860 Meise, Belgium Fernando Esteve-Raventós Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, 28805 Alcalá de Henares, Madrid, Spain Javier Angel Etayo Salazar Navarro Villoslada 16, 3º dcha., 31003 Pamplona, Navarra, Spain Vera Evenson Sam Mitchel Herbarium of Fungi, Denver Botanic Gardens, 1007 York Street, Denver, Colorado 80206, U.S.A. Guillaume Eyssartier Muséum national d’histoire naturelle, Jardin des plantes, 57 rue Cuvier, 75005 Paris, France Edit Farkas Institute of Ecology and Botany, MTA Centre for Ecological Research, 2163 Vácrátót, Hungary Alain Favre Fédération Mycologique et Botanique Dauphiné Savoie, Le Prieuré, 144 Place de l’Eglise, 74320 Sevrier, France Anna G. Fedosova Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Mario Filippa Regione Monsarinero 36, 14041 Agliano Terme, Italy Péter Finy 8000 Székesfehérvár, Zsombolyai u. 56, Hungary Adam Flakus W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland Simón Fos Facultad de Ciencias Biológicas, Universitat de València, C/Dr Moliner 50, 46100, Burjasot, Valencia, Spain Jacques Fournier Las Muros, F. 09420 Rimont, France André Fraiture Botanic Garden Meise, Nieuwelaan 38, 1860 Meise, Belgium Paolo Franchi Associazione Micologica Bresadola, Via Alessandro Volta 46, 38123 Trento, Italy Ana Esperanza Franco Molano Escuela de Microbiología, Universidad de Antioquia, AA1226, Fundación Biodiversa Colombia, Medellín, Colombia Gernot Friebes Centre of Natural History, Botany & Mycology, Universalmuseum Joanneum, Weinzöttlstraße 16, 8045 Graz, Austria Andreas Frisch NTNU, University Museum, Norwegian University of Science and Technology, 7491 Trondheim, Norway Alan Fryday Michigan State University, East Lansing, Michigan 48824, U.S.A. Giuliana Furci The Fungi Foundation, Paseo Bulnes 79 of. 112A, Santiago, Chile Ricardo Galán Márquez Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, 28805 Alcalá de Henares, Madrid, Spain Matteo Garbelotto University of California, 130 Mulford Hall #3114 Berkeley, California 94720, U.S.A. Joaquina Maria Garcia-Martin Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014, Madrid, Spain Mónica A. García Otálora Herbaria Z+ZT, ETH Zürich, CHN D37, Universitätstr. 16, 8092 Zürich, Switzerland Dania García Sánchez Universitat Rovira i Virgili, C/ Sant Llorenç 21, 43201 Reus, Tarragona, Spain Alain Gardiennet 14 rue Roulette, 21260 Véronnes, France Sigisfredo Garnica Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Isla Teja Campus, Casilla 567, Valdivia, Chile Isaac Garrido Benavent Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014, Madrid, Spain Genevieve Gates Tasmanian Institute of Agriculture, Private Bag 54, Hobart, Tasmania 7001, Australia Alice da Cruz Lima Gerlach Conservatoire et Jardin Botaniques de la ville de Genève, Genève, Switzerland Masoomeh Ghobad-Nejhad Iranian Research Organization for Science and Technology, P.O. Box 15815-3538, Tehran 15819, Iran Tatiana B. Gibertoni Universidade Federal de Pernambuco, Centro de Biociências, Avenida da Engenharia, S/N, Cidade Universitária, Recife, Pernambuco, Brazil Tine Grebenc Slovenian Forestry Institute, Vecna pot 2, 100 Ljubljana, Slovenia Irmgard Greilhuber University of Vienna, Rennweg 14, 1030 Vienna, Austria Bella Grishkan Institute of Evolution, University of Haifa, Aba Khoushi Ave. 199, Mt. Carmel, Haifa 3498838, Israel Johannes Z. Groenewald Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands Martin Grube Institute of Biology, University of Graz, Holteiasse 6, 8010 Graz, Austria Gérald Gruhn Office National des Forêts, 2 Avenue de Saint-Mandé, 75570 Paris Cedex 12, France Cécile Gueidan CSIRO — Australian National Herbarium, Clunies Ross Street, Canberra ACT 2601, Australia Gro Gulden Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway Luis FP Gusmão Universidade Estadual de Feira de Santana, Av. Transnordestina, s/n, Bairro Novo Horizonte, CEP:44036-900, Feira de Santana, Bahia, Brazil Josef Hafellner Institute of Biology, University of Graz, Holteiasse 6, 8010 Graz, Austria Michel Hairaud 2 Impasse des Marronniers, 79360 Poivendre de Marigny, France Marek Halama Museum of Natural History, Wrocław University, ul. H. Sienkiewicza 5, 50-335 Wrocław, Poland Nils Hallenberg University of Gothenburg, Box 461, 40530 Göteborg, Sweden Roy E. Halling Institute of Systematic Botany, New York Botanical Garden, 2900 Southern Blvd, Bronx, New York 10458-5126, U.S.A. Karen Hansen Swedish Museum of Natural History, P.O. Box 50007, 104 05 Stockholm, Sweden Christoffer Bugge Harder Texas Tech University, Box 42122, Lubbock, Texas 79409, U.S.A. Jacob Heilmann-Clausen Natural History Museum of Denmark, Universitetsparken 15, 2100 København, Denmark Stip Helleman Sweelinck 78, 5831KT Boxmeer, The Netherlands Alain Henriot Mycological Society of France, 20 rue Rottembourg, 12th arrondissement, Paris, France Margarita Hernandez-Restrepo Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands Raphaël Herve 24 rue des Fougères, 86550 Mignaloux-Beauvoir, France Caroline Hobart 84 Stafford Road, Sheffield, South Yorkshire S2 2SF, United Kingdom Mascha Hoffmeister Julius Kühn-Institut, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany Klaus Høiland University of Oslo, P.O.Box 1066, Blindern, 0316 Oslo, Norway Jan Holec National Museum, Herbarium PRM, Cirkusová 1740, 193 00 Praha 9, Czech Republic Håkon Holien Faculty of Bioscience and Aquaculture, NORD University, P.O. Box 2501, 7729 Steinkjer, Norway Karen Hughes University of Tennessee, Knoxville, Tennessee 37996, U.S.A. Vit Hubka Faculty of Science, Charles University, Benátská 2, 128 01 Praha 2, Czech Republic Seppo Huhtinen Herbarium TUR, Biodiversity Unit, University of Turku, 20014 Turku, Finland Boris Ivančević Natural History Museum, Njegoševa 51, P.O. Box 401, 11000 Belgrade, Serbia Marian Jagers Reelaan 13, 7522 LR Enschede, The Netherlands Walter Jaklitsch Institute of Forest Entomology, Forest Pathology and Forest Protection, University of Natural Resources and Life Sciences Vienna, Vienna, Austria AnnaElise Jansen Stationsstraat 10, 6701 AM Wageningen, the Netherlands Ruvishika S. Jayawardena Mae Fah Luang University, Chang Wat Chiang Rai 57100, Thailand Thomas Stjernegaard Jeppesen Global Biodiversity Information Facility, Universitetsparken 15, 2100 København Ø, Denmark Mikael Jeppson Lilla Håjumsgatan 4, 46135 Trollhättan, Sweden Peter Johnston Manaaki Whenua Landcare Research, Private Bag 92170, Auckland 1072, New Zealand Per Magnus Jørgensen University of Bergen, Allégaten 41, P.O. Box 7800, 5020 Bergen, Norway Ingvar Kärnefelt Biological Museum, Lund University, Box 117, 221 00 Lund, Sweden Liudmila B. Kalinina Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Gintaras Kantvilas Tasmanian Herbarium (HO), Tasmanian Museum and Art Gallery, P.O. Box 5058, UTAS LP.O., Sandy Bay, Tasmania 7005, Australia Mitko Karadelev Institute of Biology, Faculty of Natural Science and Mathematics, Ss. Cyril and Methodius University, Arhimedova 5, 1000 Skopje, Republic of Macedonia Taiga Kasuya Faculty of Risk and Crisis Management, Chiba Institute of Science, 3 Shiomi-cho, Choshi, Chiba 288-0025, Japan Ivona Kautmanová Natural History Museum, Slovak National Museum, Bratislava, Slovakia Richard W. Kerrigan RWK Research, Kittanning, Pennsylvania 16201, U.S.A. Martin Kirchmair Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25, 6020 Innsbruck, Austria Anna Kiyashko Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Dániel G. Knapp Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary Henning Knudsen Natural History Museum of Denmark, Universitetsparken 15, 2100 København, Denmark Kerry Knudsen Faculty of Environmental Sciences, University of Life Sciences at Prague, Prague, Czech Republic Tommy Knutsson Nedra Västerstad 111, 380 62 Mörbylånga, Sweden Miroslav Kolařík Institute of Microbiology ASCR, Videnska 1083, 142 20 Prague 4, Czech Republic Urmas Kõljalg Institute of Ecology and Earth Sciences, 40 Lai Street, Tartu 51005, Estonia Alica Košuthová Swedish Museum of Natural History, P.O. Box 50007, 104 05 Stockholm, Sweden Attila Koszka Faculty of Agricultural and Environmental Sciences, Kaposvar University, 7400 Kaposvar, Hungary Heikki Kotiranta Finnish Environment Institute, P.O. Box 140, 00251 Helsinki, Finland Vera Kotkova Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Ondřej Koukol Faculty of Science, Charles University, Benátská 2, 128 01 Praha 2, Czech Republic Jiří Kout University of West Bohemia, Faculty of Education, Klatovska 51, 306 19 Pilsen, Czech Republic Gábor M. Kovács Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary Martin Kříž Faculty of Science, Charles University, Benátská 2, 128 01 Praha 2, Czech Republic Åsa Kruys Museum of Evolution, Uppsala University, Norbyvägen 16, 75236 Uppsala, Sweden Viktor Kučera Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 845 23 Bratislava, Slovakia Linas Kudzma 37 Maple Ave. Annandale, New Jersey 08801, U.S.A. Francisco Kuhar Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Córdoba, Casilla de Correo 495, 5000 Córdoba, Argentina Martin Kukwa Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland T. K. Arun Kumar The Zamorin’s Guruvayurappan College, Kozhikode, Kerala 673014, India Vladimír Kunca Technical University in Zvolen, Ul. T. G. Masaryka 24, 960 53 Zvolen,Slovakia Ivana Kušan Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia Thomas W. Kuyper Wageningen University & Research, Droevendaalsesteeg 4, 6708 PB Wageningen, The Netherlands Carlos Lado Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014, Madrid, Spain Thomas Læssøe Natural History Museum of Denmark, Universitetsparken 15, 2100 København, Denmark Patrice Lainé 123 rue Saint Antoine, 75004, Paris, France Ewald Langer University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Ellen Larsson University of Gothenburg, Box 461, 40530 Göteborg, Sweden Karl-Henrik Larsson Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway Gary Laursen Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska 99775-7000, U.S.A. Christian Lechat Ascofrance, 64 route de Chizé, 79360 Villiers en Bois, France Serena Lee Herbarium Singapore Botanic Gardens, National Parks Board, Singapore James C. Lendemer (1) Institute of Systematic Botany, New York Botanical Garden, 2900 Southern Blvd, Bronx, New York 10458-5126, U.S.A.; (2) Graduate Center, City University of New York, 365 5th Ave, New York, New York 10016, U.S.A. Laura Levin University of Buenos Aires, Junin 956, 1113, Buenos Aires, Argentina Uwe Lindemann Landesmuseum für Naturkunde, Münster, Germany Håkan Lindström Östansjö 150, 840 64 Kälarne, Sweden Xingzhong Liu Institute of Microbiology, Chinese Academy of Sciences, No 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, China Regulo Carlos Llarena Hernandez Facultad de Ciencias Biológicas y Agropecuarias, Peñuela, Universidad Veracruzana, Amatlán de los Reyes, Ver., Mexico Esteve Llop Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain Csaba Locsmándi Hungarian Natural History Museum, 1087 Budapest, Hungary Deborah Jean Lodge USDA Forest Service, NRS, P.O. Box 1377, Luquillo, Puerto Rico 00773-1377, U.S.A. Michael Loizides P.O. Box 58499, 3734 Limassol, Cyprus László Lőkös Hungarian Natural History Museum, 1087 Budapest, Hungary Jennifer Luangsa-ard National Center for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, 113 Thailand Science Park, Phaholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand Matthias Lüderitz AG Geobotanik Schleswig-Holstein & Hamburg, c/o University of Kiel, Olshausenstraße 75, 24098 Kiel, Germany Thorsten Lumbsch Field Museum, 1400 S. Lake Shore Dr., Chicago, Illinois 60605, U.S.A. Matthias Lutz Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 5, 72076 Tübingen, Germany Dan Mahoney Callaghan Innovation, 69 Gracefield Road, Lower Hutt 5010, New Zealand Ekaterina Malysheva Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Vera Malysheva Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Patinjareveettil Manimohan University of Calicut, Kerala, 673 635, India Yasmina Marin-Felix Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands Guilhermina Marques Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes and Alto Douro, Laboratory of Mycology and Soil Microbiology, 5000-801 Vila Real, Portugal Rubén Martínez-Gil Ascomycete.org, 36 rue de la Garde, 69005 Lyon, France Guy Marson Musée national d’histoire naturelle, 25 rue Münster, 2160 Luxembourg, Luxembourg Gerardo Mata Instituto de Ecología, A.C., Carretera antigua a Coatepec 351, El Haya, Xalapa 91070, Veracruz, Mexico P. Brandon Matheny University of Tennessee, Knoxville, Tennessee 37996, U.S.A. Geir Harald Mathiassen Tromsø University Museum, University of Tromsø — The Arctic University of Norway, 9037 Tromsø, Norway Neven Matočec Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia Helmut Mayrhofer Institute of Biology, University of Graz, Holteiasse 6, 8010 Graz, Austria Mehdi Mehrabi Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran Ireneia Melo Botanical Garden, National Museum of Natural History and Science, University of Lisbon, Portugal Armin Mešić Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia Andrew S. Methven Savannah State University, Savannah, Georgia 31404, U.S.A Otto Miettinen Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland Ana M. Millanes Romero Universidad Rey Juan Carlos, 28933 Móstoles, Madrid, Spain Andrew N. Miller Illinois Natural History Survey, University of Illinois Urbana-Champaign, 1816 South Oak Street, Champaign, Illinois 61820-6970, U.S.A. James K. Mitchell Harvard University, 22 Divinity Avenue, Cambridge, Massachusetts 02138, U.S.A. Roland Moberg Museum of Evolution, Uppsala University, Norbyvägen 16, 75236 Uppsala, Sweden Pierre-Arthur Moreau Université Lille, Fac. Pharma. Lille, EA4483 IMPECS, 59000 Lille, France Gabriel Moreno Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, 28805 Alcalá de Henares, Madrid, Spain Olga Morozova Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Asunción Morte Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain Lucia Muggia University of Trieste, via L. Giorgieri 10, 34127 Trieste, Italy Guillermo Muñoz González Avda Valvanera N.32, 5D, 26500 Calahorra, La Rioja, Spain Leena Myllys Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland István Nagy Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary László G. Nagy Institute of Biochemistry, BRC-HAS, 6726 Szeged, Temesvari krt. 62, 6726 Szeged, Hungary Maria Alice Neves Universidade Federal de Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Trindade, Florianópolis, Santa Catarina CEP 88040-900, Brazil Tuomo Niemelä Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland Pier Luigi Nimis University of Trieste, via L. Giorgieri 10, 34127 Trieste, Italy Nicolas Niveiro Instituto de Botánica del Nordeste, Universidad Nacional de Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas, Sargento Cabral 2131, CC 209, Corrientes Capital, Argentina Machiel E. Noordeloos Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA, Leiden, The Netherlands Anders Nordin Museum of Evolution, Uppsala University, Norbyvägen 16, 75236 Uppsala, Sweden Sara Raouia Noumeur Faculty of Natural and Life Sciences, University of Batna 2, 05000 Batna, Algeria Yuri Novozhilov Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Jorinde Nuytinck Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, The Netherlands Esteri Ohenoja Botanical Museum, University of Oulu, Finland Patricia Oliveira Fiuza Universidade Estadual de Feira de Santana, Av. Transnordestina, s/n, Bairro Novo Horizonte, CEP:44036-900, Feira de Santana, Bahia, Brazil Alan Orange National Museum of Wales, Cardiff CF10 3NP, United Kingdom Alexander Ordynets University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Beatriz Ortiz-Santana USDA Forest Service, Northern Research Station, One Gifford Pinchot Dr, Madison, Wisconsin 53726, U.S.A. Leticia Pacheco Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 México, D. F., Mexico Ferenc Pál-Fám Faculty of Agricultural and Environmental Sciences, Kaposvar University, 7400 Kaposvar, Hungary Melissa Palacio Universidade Federal do Rio Grande do Sul, Brazil Zdeněk Palice Faculty of Science, Charles University, Benátská 2, 128 01 Praha 2, Czech Republic Viktor Papp Szent Istvan University, 1118 Budapest, Menesi st. 44, Hungary Kadri Pärtelv Institute of Ecology and Earth Sciences, 40 Lai Street, Tartu 51005, Estonia Julia Pawlowska Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland Aurelia Paz Urb. La Llosa, 219, 39509 Villanueva de la Peña, Mazcuerras, Cantabria, Spain Ursula Peintner Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25, 6020 Innsbruck, Austria Shaun Pennycook Manaaki Whenua Landcare Research, Private Bag 92170, Auckland 1072, New Zealand Olinto Liparini Pereira Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil Pablo Pérez Daniëls University of Córdoba, 14071, Córdoba, Spain Miquel À. Pérez-De-Gregorio Capella C/ Pau Casals, 6, 1º, 1ª, 17001, Girona, Spain Carlos Manuel Pérez del Amo C/ Luis de Ulloa, 1, 7º I, 26004 Logroño, Navarra, Spain Sergio Pérez Gorjón Universidad de Salamanca, Avda. Licenciado Mendez Nieto s/n, 37007 Salamanca, Spain Sergio Pérez-Ortega Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014, Madrid, Spain Israel Pérez-Vargas Facultad de Farmacia, Universidad de La Laguna, c/ Astrofísico Sánchez s/n 38071 La Laguna, Tenerife, Canary Islands, Spain Brian A. Perry California State University East Bay, Hayward, California 94542, U.S.A. Jens H. Petersen Nøruplundvej 2, 8400 Ebeltoft, Denmark Ronald H. Petersen University of Tennessee, Knoxville, Tennessee 37996, U.S.A. Donald H. Pfister Harvard University, 22 Divinity Avenue, Cambridge MA 02138, U.S.A. Chayanard Phukhamsakda Mae Fah Luang University, Chang Wat Chiang Rai 57100, Thailand Marcin Piątek W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland Meike Piepenbring Faculty of Biosciences, Goethe University Frankfurt am Main, Germany Raquel Pino-Bodas Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014, Madrid, Spain Juan Pablo Pinzón Esquivel Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, Apdo. Postal: 4-116 Itzimná,C.P: 97100, Merida, Yucatan, Mexico Paul Pirot Rue des Peupliers 10, 6840 Neufchâteau, Belgium Eugene S. Popov Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Orlando Popoff Instituto de Botánica del Nordeste, Universidad Nacional de Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas, Sargento Cabral 2131, CC 209, Corrientes Capital, Argentina María Prieto Álvaro Universidad Rey Juan Carlos, 28933 Móstoles, Madrid, Spain Christian Printzen Senckenberg Forschungsinstitut und Naturmuseum Frankfurt, Abteilung Botanik und Molekulare Evolutionsforschung, Herbarium Senckenbergianum (FR), Senckenberganlage 25, 60325 Frankfurt am Main, Germany Nadezhda Psurtseva Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Witoon Purahong Helmholtz Centre for Environmental Research GmbH — UFZ, Theodor-Lieser-Straße 4, 06120 Halle, Germany Luis Quijada Harvard University, 22 Divinity Avenue, Cambridge, Massachusetts 02138, U.S.A. Gerhard Rambold University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany Natalia A. Ramírez Instituto de Botánica del Nordeste, Universidad Nacional de Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas, Sargento Cabral 2131, CC 209, Corrientes Capital, Argentina Huzefa Raja University of North Carolina at Greensboro, 435 Sullivan Science Building, PO Box 26170, Greensboro North Carolina 27402-6170, U.S.A. Olivier Raspé Botanic Garden Meise, Nieuwelaan 38, 1860 Meise, Belgium Tania Raymundo Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Miguel Hidalgo, Santo Tomás, 11340 Ciudad de México, Mexico Martina Réblová Institute of Botany, Czech Academy of Sciences, Pruhonice, Czech Republic Yury A. Rebriev Southern Scientific Center, Russian Academy of Sciences, 41 Chehova str., Rostov-on-Don, 344006, Russia Juan de Dios Reyes García Paseo Virgen de Linarejos 6 2 D, Linares, Jaen, Spain Miguel Ángel Ribes Ripoll Avda. Pablo Neruda 120 F, 2°D, 28018 Madrid, Spain Franck Richard CEFE UMR5175, CNRS, Université de Montpellier, Université Paul-Valéry Montpellier, EPHE, INSERM, 1919 Route de Mende, 34293 Montpellier Cédex 5, France Mike J. Richardson Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh, EH3 5LR, United Kingdom Víctor J. Rico Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain Gerardo Lucio Robledo Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Córdoba, Casilla de Correo 495, 5000 Córdoba, Argentina Flavia Rodrigues Barbosa Universidade Federal de Mato Grosso, Av. Alexandre Ferronato, 1200, Setor Industrial, Sinop, Mato Grosso, Brazil Cristina Rodriguez-Caycedo UT Southwestern Medical Center, Dallas, Texas 75390, U.S.A. Pamela Rodriguez-Flakus W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland Anna Ronikier W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland Luis Rubio Casas Ul. Andrieja Sacharowa 1/1, 30-806 Kraków, Poland Katerina Rusevska Institute of Biology, Faculty of Natural Science and Mathematics, Ss. Cyril and Methodius University, Arhimedova 5, 1000 Skopje, Republic of Macedonia Günter Saar Dammenmühle 7, 77933 Lahr-Sulz, Germany Irja Saar Institute of Ecology and Earth Sciences, 40 Lai Street, Tartu 51005, Estonia Isabel Salcedo University of the Basque Country (UPV/EHU), Apdo 644, 48080 Bilbao, Spain Sergio M. Salcedo Martínez Facultad de Ciencias Biológicas, UANL. Ave. Pedro de Alba s/n esq. Manuel Barragán Cd. Universitaria, San Nicolás de los Garza Nuevo León, CP. 66451, Mexico Carlos A. Salvador Montoya Instituto de Botánica del Nordeste, Universidad Nacional de Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas, Sargento Cabral 2131, CC 209, Corrientes Capital, Argentina Santiago Sánchez-Ramírez University of Toronto, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada J. Vladimir Sandoval-Sierra Health Science Centre, University of Tennessee, U.S.A. Sergi Santamaria Facultat de Biociències, Edifici C, Despatx C1/331, Campus de la UAB, 08193 Bellaterra, (Cerdanyola del Vallès), Barcelona, Spain Josiane Santana Monteiro Botany Coordination, Museu Paraense Emílio Goeldi, 66077-830, Belém, Pará, Brazil Hans Josef Schroers Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia Barbara Schulz Institute of Microbiology, Technische Universität Braunschweig, Germany Geert Schmidt-Stohn Burgstr. 25, 29553 Bienenbüttel, Germany Trond Schumacher University of Oslo, P.O.Box 1066, Blindern, 0316 Oslo, Norway Beatrice Senn-Irlet RU Biodiversity and Conservation Biology, Swiss Federal Institute for Forest, Snow and Landscape Research WSL, Zürcherstr. 111, 8903 Birmensdorf, Switzerland Hana Ševčíková Moravian Museum, Zeny trh 6, 659 37 Brno, Czech Republic Oleg Shchepin Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Takashi Shirouzu Graduate School of Bioresources, Mie University, 1577 Kurima-machiya, Tsu, Mie 514-8507, Japan Anton Shiryaev Institute of Plant & Animal Ecology Ural Branch of the Russian Academy of Sciences, 620144 Ekaterinburg, 8 March str., 202/3, Russia Klaus Siepe Geeste 133, 46342 Velen, Germany Esteban B. Sir Fundación Miguel Lillo, Consejo Nacional de Investigaciones Científicas y Técnicas, Miguel Lillo 251, San Miguel de Tucumán 4000, Tucumán, Argentina Mohammad Sohrabi Iranian Research Organization for Science and Technology, P.O. Box 33535111, Tehran, Iran Karl Soop Swedish Museum of Natural History, P.O. Box 50007, 104 05 Stockholm, Sweden Viacheslav Spirin Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland Toby Spribille University of Alberta, Edmonton, Alberta T6G 2R3, Canada Marc Stadler Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany Joost Stalpers Torenlaan 43, 3742CR Baarn, The Netherlands Soili Stenroos Finnish Museum of Natural History, P.O. Box 7, 00014 University of Helsinki, Finland Ave Suija Institute of Ecology and Earth Sciences, 40 Lai Street, Tartu 51005, Estonia Stellan Sunhede Hökaskog Sandbacken 1, 533 92 Lundsbrunn, Sweden Sten Svantesson University of Gothenburg, Box 461, 40530 Göteborg, Sweden Sigvard Svensson Biological Museum, Lund University, Box 117, 221 00 Lund, Sweden Tatyana Yu. Svetasheva (1) Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia; (2) Department of Technologies of Living Systems, Tula State Lev Tolstoy Pedagogical University, Lenin ave. 125, Tula, 300026, Russia Krzysztof Świerkosz Museum of Natural History, Wrocław University, ul. H. Sienkiewicza 5, 50-335 Wrocław, Poland Heidi Tamm Institute of Ecology and Earth Sciences, 40 Lai Street, Tartu 51005, Estonia Hatira Taskin Faculty of Agriculture, University of Çukurova, 01330 Adana, Turkey Adrien Taudière CEFE UMR5175, CNRS, Université de Montpellier, Université Paul-Valéry Montpellier, EPHE, INSERM, 1919 Route de Mende, 34293 Montpellier Cédex 5, France Jan-Olof Tedebrand Sundsvall Mycological Society, Medelpad, Sweden Raúl Tena Lahoz C/Arreñales del Portillo B 21 1°D, 44003 Teruel, Spain Marina Temina Institute of Evolution, University of Haifa, Aba Khoushi Ave. 199, Mt. Carmel, Haifa 3498838, Israel Arne Thell Biological Museum, Lund University, Box 117, 221 00 Lund, Sweden Marco Thines Faculty of Biosciences, Goethe University Frankfurt am Main, Germany Göran Thor Swedish University of Agricultural Sciences, P. O. Box 7044, 750 07 Uppsala, Sweden Holger Thüs State Museum of Natural History Stuttgart, Rosenstein 1, 70191 Stuttgart, Germany Leif Tibell Evolutionary Biology Centre, Norbyvägen 18D, 75236 Uppsala, Sweden Sanja Tibell Evolutionary Biology Centre, Norbyvägen 18D, 75236 Uppsala, Sweden Einar Timdal Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway Zdenko Tkalčec Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia Tor Tønsberg University of Bergen, Allégaten 41, P.O. Box 7800, 5020 Bergen, Norway Gérard Trichies 5, impasse des Écoles S7700 Neufchef, France Dagmar Triebel Botanische Staatssammlung München, Menzinger Straße 67, 80638 München, Germany Andrei Tsurykau (1) F. Skorina Gomel State University, Sovetskaja Str. 104, 246019 Gomel, Belarus; (2) Institute of Natural Sciences, Samara National Research University, Moskovskoye shosse 34, 443086 Samara, Russia Rodham E. Tulloss Herbarium Amanitarum Rooseveltensis, P. O. Box 57, Roosevelt, New Jersey 08555-0057, U.S.A. Veera Tuovinen University of Alberta, Edmonton, Alberta T6G 2R3, Canada Miguel Ulloa Sosa Universidad Nacional Autónoma de México, Tercer Circuito/Sin Número, Ciudad Universitaria, Ciudad de México, C. P. 04510, Mexico Carlos Urcelay Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Córdoba, Casilla de Correo 495, 5000 Córdoba, Argentina François Valade 11 rue haras, le boqueteau, 91240 Saint-Michel-sur-Orge, France Ricardo Valenzuela Garza Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Miguel Hidalgo, Santo Tomás, 11340 Ciudad de México, Mexico Pieter van den Boom Arafura 16, 5691 JA Son, The Netherlands Nicolas Van Vooren Ascomycete.org, 36 rue de la Garde, 69005 Lyon, France Aida M. Vasco-Palacios Escuela de Microbiología, Universidad de Antioquia, AA1226, Fundación Biodiversa Colombia, Medellín, Colombia Jukka Vauras Herbarium TUR, Biodiversity Unit, University of Turku, 20014 Turku, Finland Juan Manuel Velasco Santos 3 C/Pontevedra, 18, 1º C, 37003 Salamanca, Spain Else Vellinga 861 Keeler Avenue, Berkeley, California 94708, U.S.A. Annemieke Verbeken Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium Per Vetlesen Norges Sopp- og Nyttevekstforbund, Schweigaards gate 34F, 0191 Oslo, Norway Alfredo Vizzini University of Torino, Viale P.A. Mattioli 25, 10125, Torino, Italy Hermann Voglmayr University of Vienna, Rennweg 14, 1030 Vienna, Austria Sergey Volobuev Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia Wolfgang von Brackel Kirchenweg 2, 91341 Röttenbach, Germany Elena Voronina Lomonosov Moscow State University, Biology Faculty, Moscow, Russia Grit Walther Arvid-Harnack-Str. 4, 07743 Jena, Germany Roy Watling Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh, EH3 5LR, United Kingdom Evi Weber Blaihofstr. 42, 72074 Tübingen, Germany Mats Wedin Swedish Museum of Natural History, P.O. Box 50007, 104 05 Stockholm, Sweden Øyvind Weholt Nord University, Nesna, 8700 Nesna, Norway Martin Westberg Museum of Evolution, Uppsala University, Norbyvägen 16, 75236 Uppsala, Sweden Eugene Yurchenko Polessky State University, Dnyaprouskai flatylii str. 23, 225710, Pinsk, Belarus Petr Zehnálek Faculty of Science, Charles University, Benátská 2, 128 01 Praha 2, Czech Republic Huang Zhang Kunming University of Science & Technology, Kunming, China Mikhail P. Zhurbenko Komarov Botanical Institute of the Russian Academy of Sciences, 2 Prof. Popov Street, St. Petersburg, 197376, Russia The following institutions are also supporting the present text (institutional support means that committees from the mentioned) Asociación Micológica Carlos Spegazzini (Argentina) Austrian Mycological Society (Austria) Croatian Mycological Society (Croatia) Committee of the Czech Scientific Society for Mycology (Czech Republic) The Netherlands Mycological Society (The Netherlands) Sociedad Española de Liquenología (Portugal/Spain) Iberian Mycological Society (which is under constitution, Portugal/Spain) Federación de Asociaciones Micológicas Andaluzas (FAMA) (Spain) Asociación Botánica y Micológica de Jaén (Spain) Asociación Micológica Hispalense Muscaria (Spain) Societat Micològica Valenciana (Spain)
- Published
- 2018
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