1. Study on the Interaction between the Inclusion Complex of Hematoxylin with β-Cyclodextrin and DNA.
- Author
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Xu, Dongling, Wang, Xingming, Fei, Dan, and Ding, Lisheng
- Subjects
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CYCLODEXTRINS , *PLANT extracts , *COMPLEX compounds , *OPTICAL spectroscopy , *FLUORESCENCE spectroscopy , *ELECTROCHEMISTRY , *THERMODYNAMICS , *BINDING sites , *BIOCOMPLEXITY - Abstract
Ultraviolet-visible (UV-vis) spectra, fluorescence spectra, electrochemistry, and the thermodynamic method were used to discuss the interaction mode between the inclusion complex of hematoxylin with β-cyclodextrin and herring sperm DNA. On the condition of physiological pH, the result showed that hematoxylin and β-cyclodextrin formed an inclusion complex with binding ratio nhematoxylin:nβ-cyclodextrin = 1:1. The interaction mode between β-cyclodextrin-hematoxylin and DNA was a mixed binding, which contained intercalation and electrostatic mode. The binding ratio between β-cyclodextrin-hematoxylin and DNA was nβ-cyclodextrin -hematoxylin:nDNA = 2:1, binding constant was K⊖298.15K = 5.29 × 104 L·mol-1, and entropy worked as driven force in this action. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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