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1. Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. I: Assessment of different separation concepts.

Author

Marek, Wojciech, Muca, Renata, Woś, Sylwia, Piątkowski, Wojciech, and Antos, Dorota

Subjects
*MONOCLONAL antibodies, *IMMUNOGLOBULIN G, *HAMSTERS as laboratory animals, *CHEMICAL purification, *SEPARATION (Technology)
Abstract

Highlights: [•] Monoclonal anybody IgG1 was isolated out of a CHO derived supernatant. [•] Different purification techniques were used based on IEC, HIC and ATPE. [•] The best performance was achieved by integrating IEC and HIC. [•] The on-column dilution was used for feed loading in the integrated process. [ABSTRACT FROM AUTHOR]

Published
2013
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