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Abstract: Huperzine A (HupA) and Huperzine B (HupB) are natural alkaloids existed in Lycopodium plants. They both have potential clinical application for treating Alzheimer''s Disease (AD). For the purpose of better utilizing the limited plant resources, a quick and low cost method to separate and purify HupA and HupB from Huperzia serrata (Thunb. ex Murray) was established in this paper. Low polarity macroporous resin SP850 was selected from eight kinds of resins during initial purification. Trifluoroacetic acid (TFA) was proved to be the best acid modifier reagent among all acids used in our experiment for improving separation. HupA and HupB were baseline separated on a C18 column by preparative high performance liquid chromatography (Preparative HPLC), the optimal gradient mobile phase system contained methanol increasing from 15% (v/v) to 35% (v/v) and 0.1% (v/v) TFA within the water. The purity of HupA and HupB obtained was 99.1% and 98.6%, respectively, and the total recovery for them was 83.0% and 81.8%, respectively. [Copyright &y& Elsevier]

Abstract: A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high-speed counter-current chromatography (HSCCC) separation. Six alkaloids were separated and purified simultaneously with n-hexane–ethyl acetate–methanol–water (2:5:2:5) on HSCCC in one step. As a result, 4.7mg palmatine, 7.1mg berberine, 0.8mg worenine, 1.5mg epiberberine, 1.9mg coptisine and 0.6mg jatrorrhizine from 20mg Rhizoma coptidis extract were obtained with the purities of 98.5%, 94.1%, 90.4%, 95.5%, 88.4% and 91.1% respectively as determined by high-performance liquid chromatography (HPLC), and they were characterized by MS and 1H NMR. [Copyright &y& Elsevier]