23 results on '"Yan, Jun"'
Search Results
2. Genome‑wide identification, phylogenetic and expression pattern analysis of GATA family genes in foxtail millet (Setaria italica)
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Lai, Dili, Yao, Xin, Yan, Jun, Gao, Anjing, Yang, Hao, Xiang, Dabing, Ruan, Jingjun, Fan, Yu, and Cheng, Jianping
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- 2022
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3. Genome-wide identification and expression analysis of the bHLH transcription factor family and its response to abiotic stress in foxtail millet (Setaria italica L.)
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Fan, Yu, Lai, Dili, Yang, Hao, Xue, Guoxing, He, Ailing, Chen, Long, Feng, Liang, Ruan, Jingjun, Xiang, Dabing, Yan, Jun, and Cheng, Jianping
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- 2021
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4. Genome-wide identification, expression analysis, and functional study of the GRAS transcription factor family and its response to abiotic stress in sorghum [Sorghum bicolor (L.) Moench]
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Fan, Yu, Yan, Jun, Lai, Dili, Yang, Hao, Xue, Guoxing, He, Ailing, Guo, Tianrong, Chen, Long, Cheng, Xiao-bin, Xiang, Da-bing, Ruan, Jingjun, and Cheng, Jianping
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- 2021
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5. Genome-wide identification and expression analysis of the bHLH transcription factor family and its response to abiotic stress in sorghum [Sorghum bicolor (L.) Moench]
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Fan, Yu, Yang, Hao, Lai, Dili, He, Ailing, Xue, Guoxing, Feng, Liang, Chen, Long, Cheng, Xiao-bin, Ruan, Jingjun, Yan, Jun, and Cheng, Jianping
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- 2021
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6. Genome-wide investigation of the GRAS transcription factor family in foxtail millet (Setaria italica L.)
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Fan, Yu, Wei, Xiaobao, Lai, Dili, Yang, Hao, Feng, Liang, Li, Long, Niu, Kexin, Chen, Long, Xiang, Dabing, Ruan, Jingjun, Yan, Jun, and Cheng, Jianping
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- 2021
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7. The NADPH oxidase in Volvariella volvacea and its differential expression in response to mycelial ageing and mechanical injury
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Yan, Jun-Jie, Tong, Zong-Jun, Liu, Yuan-Yuan, Lin, Zi-Yan, Long, Ying, Han, Xing, Xu, Wei-Nan, Huang, Qian-Hui, Tao, Yong-Xin, and Xie, Bao-Gui
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- 2020
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8. Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses
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Ryu, Moon Young, Cho, Seok Keun, Hong, Yourae, Kim, Jinho, Kim, Jong Hum, Kim, Gu Min, Chen, Yan-Jun, Knoch, Eva, Møller, Birger Lindberg, Kim, Woo Taek, Lyngkjær, Michael Foged, and Yang, Seong Wook
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- 2019
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9. Phylogeny of the plant receptor-like kinase (RLK) gene family and expression analysis of wheat RLK genes in response to biotic and abiotic stresses.
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Yan, Jun, Su, Peisen, Meng, Xianyong, and Liu, Pingzeng
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GENE families , *GENE expression , *ABIOTIC stress , *PHYLOGENY , *WHEAT , *BRACHYPODIUM - Abstract
Background: The receptor-like kinase (RLK) gene families in plants contains a large number of members. They are membrane proteins with an extracellular receptor domain and participate in biotic and abiotic stress responses. Results: In this study, we identified RLKs in 15 representative plant genomes, including wheat, and classified them into 64 subfamilies by using four types of phylogenetic trees and HMM models. Conserved exon‒intron structures with conserved exon phases in the kinase domain were found in many RLK subfamilies from Physcomitrella patens to Triticum aestivum. Domain distributions of RLKs were also diagrammed. Collinearity events and tandem gene clusters suggested that polyploidization and tandem duplication events contributed to the member expansions of T. aestivum RLKs. Global expression pattern analysis was performed by using public transcriptome data. These analyses were involved in T. aestivum, Aegilops tauschii and Brachypodium distachyon RLKs under biotic and abiotic stresses. We also selected 9 RLKs to validate the transcriptome prediction by using qRT‒PCR under drought treatment and with Fusarium graminearum infection. The expression trends of these 9 wheat RLKs from public transcriptome data were consistent with the results of qRT‒PCR, indicating that they might be stress response genes under drought or F. graminearum treatments. Conclusion: In this study, we identified, classified, evolved, and expressed RLKs in wheat and related plants. Thus, our results will provide insights into the evolutionary history and molecular mechanisms of wheat RLKs. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses
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Woo Taek Kim, Seong Wook Yang, Michael F. Lyngkjær, Moon Young Ryu, Jinho Kim, Seok Keun Cho, Yan-Jun Chen, Gu Min Kim, Yourae Hong, Birger Lindberg Møller, Eva Knoch, and Jong Hum Kim
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0106 biological sciences ,lcsh:QH426-470 ,Ubiquitin-Protein Ligases ,lcsh:Biotechnology ,Arabidopsis ,01 natural sciences ,Host-Parasite Interactions ,03 medical and health sciences ,Ascomycota ,Biotic stress ,Gene Expression Regulation, Plant ,Barley ,lcsh:TP248.13-248.65 ,Genetics ,Plant defense against herbivory ,Amino Acid Sequence ,Ubiquitin proteasome system (UPS) ,Phylogeny ,Plant Proteins ,030304 developmental biology ,Hordeum vulgare ,0303 health sciences ,Expressed sequence tag ,biology ,Abiotic stress ,food and beverages ,Hordeum ,Oryza ,biology.organism_classification ,Droughts ,Ubiquitin ligase ,lcsh:Genetics ,Proteasome ,Seedlings ,biology.protein ,Sequence Alignment ,Genome, Plant ,Research Article ,010606 plant biology & botany ,Biotechnology - Abstract
Background Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role in subjecting proteins to hydrolysis by the UPS. Recently, it has been demonstrated that a specific class of E3 ligases termed the U-box ligases are directly associated with the defense mechanisms against abiotic and biotic stresses in several plants. However, no studies on U-box E3 ligases have been performed in one of the important staple crops, barley. Results In this study, we identified 67 putative U-box E3 ligases from the barley genome and expressed sequence tags (ESTs). Similar to Arabidopsis and rice U-box E3 ligases, most of barley U-box E3 ligases possess evolutionary well-conserved domain organizations. Based on the domain compositions and arrangements, the barley U-box proteins were classified into eight different classes. Along with this new classification, we refined the previously reported classifications of U-box E3 ligase genes in Arabidopsis and rice. Furthermore, we investigated the expression profile of 67 U-box E3 ligase genes in response to drought stress and pathogen infection. We observed that many U-box E3 ligase genes were specifically up-and-down regulated by drought stress or by fungal infection, implying their possible roles of some U-box E3 ligase genes in the stress responses. Conclusion This study reports the classification of U-box E3 ligases in barley and their expression profiles against drought stress and pathogen infection. Therefore, the classification and expression profiling of barley U-box genes can be used as a platform to functionally define the stress-related E3 ligases in barley. Electronic supplementary material The online version of this article (10.1186/s12864-019-5696-z) contains supplementary material, which is available to authorized users.
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- 2019
11. Genome-wide identification, phylogenetic and expression pattern analysis of MADS-box family genes in foxtail millet (Setaria italica).
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Lai, Dili, Yan, Jun, He, Ailing, Xue, Guoxing, Yang, Hao, Feng, Liang, Wei, Xiaobao, Li, Long, Xiang, Dabing, Ruan, Jingjun, Fan, Yu, and Cheng, Jianping
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FOXTAIL millet , *GENE families , *ARABIDOPSIS thaliana , *ABIOTIC stress , *MONOCOTYLEDONS - Abstract
Foxtail millet (Setaria italica) is rich in nutrients and extremely beneficial to human health. We identified and comprehensively analyzed 89 MADS-box genes in the foxtail millet genome. According to the classification of MADS-box genes in Arabidopsis thaliana and rice, the SiMADS-box genes were divided into M-type (37) and MIKC-type (52). During evolution, the differentiation of MIKC-type MADS-box genes occurred before that of monocotyledons and dicotyledons. The SiMADS-box gene structure has undergone much differentiation, and the number of introns in the MIKC-type subfamily is much greater than that in the M-type subfamily. Analysis of gene duplication events revealed that MIKC-type MADS-box gene segmental duplication accounted for the vast majority of gene duplication events, and MIKC-type MADS-box genes played a major role in the amplification of SiMADS-box genes. Collinearity analysis showed highest collinearity between foxtail millet and maize MADS-box genes. Analysis of tissue-specific expression showed that SiMADS-box genes are highly expressed throughout the grain-filling process. Expression analysis of SiMADS-box genes under eight different abiotic stresses revealed many stress-tolerant genes, with induced expression of SiMADS33 and SiMADS78 under various stresses warranting further attention. Further, some SiMADS-box proteins may interact under external stress. This study provides insights for MADS-box gene mining and molecular breeding of foxtail millet in the future. [ABSTRACT FROM AUTHOR]
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- 2022
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12. Fe induces a dynamic and biased allocation of material flux within terpenoid metabolism controlled by CbNudix in Conyza blinii.
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Zheng, Tianrun, Zhan, Junyi, Wang, Maojia, Sun, Wenjun, Yan, Jun, Shan, Zhi, and Chen, Hui
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SAPONINS ,METABOLIC regulation ,CHINESE medicine ,ABIOTIC stress ,LIQUID chromatography ,GENE expression - Abstract
Background and aims: Conyza blinii is a unique traditional Chinese medicine growing in Sichuan, China, which has soils with an abundant iron content. This Fe-enriched environment contributes to a variety of terpenoids in C. blinii, such as blinin and saponin, which play an important role in the process of resisting abiotic stress. The relationship between Fe and terpenoid metabolism was studied to explore the Fe tolerance mechanism of C. blinii. Methods: In this study, C. blinii was treated with ferrous iron solutions, and the effect of ferrous iron on the synthesis of blinin and saponins was further studied by spectrophotometry and liquid chromatography. Additionally, gene expression was detected by qRT-PCR. Results: Under ferrous treatment, the blinin content of C. blinii increased, while the total saponin content decreased. When the ferrous concentration reached 200 μM, the difference in metabolite production was the largest. Furthermore, it was found that blinin and saponins have synchronous and opposite accumulation trends, characterized by time dependence. The gene expression results of key enzymes in the MVA and MEP pathways showed the same trends. In this process, the expression of CbNudixs played a key role in switching the material flux between MVA and MEP by catalyzing the dephosphorylation of isoprenoid diphosphate. Conclusions: In this study, it was found that under ferrous iron stimulation, the terpenoid content in C. blinii was different, and the metabolic pathways of MVA and MEP were periodically regulated. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings
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Junmei Kang, Tiejun Zhang, Yan-jun Cui, Qiao-li Ma, Qingchuan Yang, Ruicai Long, Xiong Junbo, and Yan Sun
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0301 basic medicine ,Osmotic shock ,Agriculture (General) ,Plant Science ,Biology ,Proteomics ,Photosynthesis ,Biochemistry ,seedling growth ,S1-972 ,03 medical and health sciences ,proteomics ,Food Animals ,PEG ratio ,Botany ,Protein biosynthesis ,salinity stress ,Ecology ,Abiotic stress ,food and beverages ,Metabolism ,biology.organism_classification ,030104 developmental biology ,Seedling ,Animal Science and Zoology ,osmotic stress ,Agronomy and Crop Science ,alfalfa ,Food Science - Abstract
Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L−1 NaCl and 180 g L−1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P
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- 2016
14. Research Progress on Phytopathogenic Fungi and Their Role as Biocontrol Agents.
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Peng, Yan, Li, Shi J., Yan, Jun, Tang, Yong, Cheng, Jian P., Gao, An J., Yao, Xin, Ruan, Jing J., and Xu, Bing L.
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PHYTOPATHOGENIC fungi ,AGRICULTURAL pests ,BIOLOGICAL pest control agents ,SUSTAINABLE agriculture ,BIOLOGICAL weed control ,FUNGAL viruses ,PLANT parasites - Abstract
Phytopathogenic fungi decrease crop yield and quality and cause huge losses in agricultural production. To prevent the occurrence of crop diseases and insect pests, farmers have to use many synthetic chemical pesticides. The extensive use of these pesticides has resulted in a series of environmental and ecological problems, such as the increase in resistant weed populations, soil compaction, and water pollution, which seriously affect the sustainable development of agriculture. This review discusses the main advances in research on plant-pathogenic fungi in terms of their pathogenic factors such as cell wall-degrading enzymes, toxins, growth regulators, effector proteins, and fungal viruses, as well as their application as biocontrol agents for plant pests, diseases, and weeds. Finally, further studies on plant-pathogenic fungal resources with better biocontrol effects can help find new beneficial microbial resources that can control diseases. [ABSTRACT FROM AUTHOR]
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- 2021
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15. Expression patterns of members of the ethylene signaling–related gene families in response to dehydration stresses in cassava
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Hou Rui Shi, Xiao Guan, Xi Yan Zhang, Tian Yan Yun, Yin Dong Zhang, Jingyi Wang, Cheng Liang Li, Ren Jun Feng, Yan Jun Chen, Jiang Hui Xie, Ming Peng, Heng Zhang, Li Fang Lu, Peng He, and Meng Yun Ren
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0106 biological sciences ,0301 basic medicine ,Leaves ,Manihot ,Time Factors ,lcsh:Medicine ,Gene Expression ,Plant Science ,Disaccharides ,01 natural sciences ,Biochemistry ,Plant Roots ,chemistry.chemical_compound ,Plant Resistance to Abiotic Stress ,Gene expression ,Plant Hormones ,lcsh:Science ,Phylogeny ,Plant Proteins ,Abiotic component ,Multidisciplinary ,Ecology ,Dehydration ,Organic Compounds ,Plant Biochemistry ,Plant Anatomy ,Plant physiology ,food and beverages ,Plants ,Chemistry ,Experimental Organism Systems ,Plant Physiology ,Physical Sciences ,Research Article ,Signal Transduction ,Proline ,Arabidopsis Thaliana ,Drought tolerance ,Carbohydrates ,Brassica ,Biology ,Research and Analysis Methods ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Ethylene ,Model Organisms ,Plant and Algal Models ,Stress, Physiological ,Plant-Environment Interactions ,Botany ,Osmotic Shock ,DNA-binding proteins ,Genetics ,Gene Regulation ,Plant Defenses ,Computer Simulation ,Gene ,Cassava ,Abiotic stress ,Plant Ecology ,Gene Expression Profiling ,lcsh:R ,fungi ,Organic Chemistry ,Ecology and Environmental Sciences ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Trehalose ,Cell Biology ,Plant Pathology ,Ethylenes ,Hormones ,Regulatory Proteins ,Gene expression profiling ,Plant Leaves ,030104 developmental biology ,chemistry ,lcsh:Q ,Shrubs ,010606 plant biology & botany ,Transcription Factors - Abstract
Drought is the one of the most important environment stresses that restricts crop yield worldwide. Cassava (Manihot esculenta Crantz) is an important food and energy crop that has many desirable traits such as drought, heat and low nutrients tolerance. However, the mechanisms underlying drought tolerance in cassava are unclear. Ethylene signaling pathway, from the upstream receptors to the downstream transcription factors, plays important roles in environmental stress responses during plant growth and development. In this study, we used bioinformatics approaches to identify and characterize candidate Manihot esculenta ethylene receptor genes and transcription factor genes. Using computational methods, we localized these genes on cassava chromosomes, constructed phylogenetic trees and identified stress-responsive cis-elements within their 5' upstream regions. Additionally, we measured the trehalose and proline contents in cassava fresh leaves after drought, osmotic, and salt stress treatments, and then it was found that the regulation patterns of contents of proline and trehalose in response to various dehydration stresses were differential, or even the opposite, which shows that plant may take different coping strategies to deal with different stresses, when stresses come. Furthermore, expression profiles of these genes in different organs and tissues under non-stress and abiotic stress were investigated through quantitative real-time PCR (qRT-PCR) analyses in cassava. Expression profiles exhibited clear differences among different tissues under non-stress and various dehydration stress conditions. We found that the leaf and tuberous root tissues had the greatest and least responses, respectively, to drought stress through the ethylene signaling pathway in cassava. Moreover, tuber and root tissues had the greatest and least reponses to osmotic and salt stresses through ethylene signaling in cassava, respectively. These results show that these plant tissues had differential expression levels of genes involved in ethylene signaling in response to the stresses tested. Moreover, after several gene duplication events, the spatiotemporally differential expression pattern of homologous genes in response to abiotic and biotic stresses may imply their functional diversity as a mechanism for adapting to the environment. Our data provide a framework for further research on the molecular mechanisms of cassava resistance to drought stress and provide a foundation for breeding drought-resistant new cultivars.
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- 2017
16. Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance.
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Sun, Shi-Chao, Xiong, Xian-Peng, Zhang, Xiao-Li, Feng, Hong-Jie, Zhu, Qian-Hao, Sun, Jie, and Li, Yan-Jun
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ABIOTIC stress ,DROUGHT tolerance ,GENE families ,COTTON ,GENE expression profiling ,SOIL enzymology ,FLOWERING of plants - Abstract
Background: The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress. Results: We performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis-elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 (including Gh4CL7) were up-regulated under the polyethylene glycol (PEG) simulated drought stress conditions. Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7-silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H
2 O2 ). Arabidopsis lines overexpressing Gh4CL7, however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H2 O2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7-silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a ~ 20% reduction and a ~ 10% increase in lignin content, respectively. The expression levels of genes related to lignin biosynthesis, including PAL, CCoAOMT, COMT, CCR and CAD, were lower in Gh4CL7-silencing plants than in controls. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton. Conclusion: We characterized the 4CL gene family in upland cotton and revealed a role of Gh4CL7 in lignin biosynthesis and drought tolerance. [ABSTRACT FROM AUTHOR]- Published
- 2020
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17. The miR165/166 Mediated Regulatory Module Plays Critical Roles in ABA Homeostasis and Response in Arabidopsis thaliana.
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Yan, Jun, Zhao, Chunzhao, Zhou, Jianping, Yang, Yu, Wang, Pengcheng, Zhu, Xiaohong, Tang, Guiliang, Bressan, Ray A., and Zhu, Jian-Kang
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HOMEOSTASIS , *PLANT growth , *GERMINATION , *PLANT development , *ABIOTIC stress - Abstract
The function of miR165/166 in plant growth and development has been extensively studied, however, its roles in abiotic stress responses remain largely unknown. Here, we report that reduction in the expression of miR165/166 conferred a drought and cold resistance phenotype and hypersensitivity to ABA during seed germination and post-germination seedling development. We further show that the ABA hypersensitive phenotype is associated with a changed transcript abundance of ABA-responsive genes and a higher expression level of ABI4, which can be directly regulated by a miR165/166 target. Additionally, we found that reduction in miR165/166 expression leads to elevated ABA levels, which occurs at least partially through the increased expression of BG1, a gene that is directly regulated by a miR165/166 target. Taken together, our results uncover a novel role for miR165/166 in the regulation of ABA and abiotic stress responses and control of ABA homeostasis. [ABSTRACT FROM AUTHOR]
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- 2016
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18. Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat.
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Dong, Yan, Liu, Jindong, Zhang, Yan, Geng, Hongwei, Rasheed, Awais, Xiao, Yonggui, Cao, Shuanghe, Fu, Luping, Yan, Jun, Wen, Weie, Zhang, Yong, Jing, Ruilian, Xia, Xianchun, and He, Zhonghu
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COMPOSITION of wheat ,WHEAT varieties ,WHEAT breeding ,GENOMES ,CARBOHYDRATES ,ABIOTIC stress ,PHENOTYPES - Abstract
Water soluble carbohydrates (WSC) in stems play an important role in buffering grain yield in wheat against biotic and abiotic stresses; however, knowledge of genes controlling WSC is very limited. We conducted a genome-wide association study (GWAS) using a high-density 90K SNP array to better understand the genetic basis underlying WSC, and to explore marker-based breeding approaches. WSC was evaluated in an association panel comprising 166 Chinese bread wheat cultivars planted in four environments. Fifty two marker-trait associations (MTAs) distributed across 23 loci were identified for phenotypic best linear unbiased estimates (BLUEs), and 11 MTAs were identified in two or more environments. Liner regression showed a clear dependence of WSC BLUE scores on numbers of favorable (increasing WSC content) and unfavorable alleles (decreasing WSC), indicating that genotypes with higher numbers of favorable or lower numbers of unfavorable alleles had higher WSC content. In silico analysis of flanking sequences of trait-associated SNPs revealed eight candidate genes related to WSC content grouped into two categories based on the type of encoding proteins, namely, defense response proteins and proteins triggered by environmental stresses. The identified SNPs and candidate genes related to WSC provide opportunities for breeding higher WSC wheat cultivars. [ABSTRACT FROM AUTHOR]
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- 2016
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19. Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
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Hou, Yueh-Ju, Zhu, Yingfang, Wang, Pengcheng, Zhao, Yang, Xie, Shaojun, Batelli, Giorgia, Wang, Bangshing, Duan, Cheng-Guo, Wang, Xingang, Xing, Lu, Lei, Mingguang, Yan, Jun, Zhu, Xiaohong, and Zhu, Jian-Kang
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ABSCISIC acid ,PHOSPHOPROTEIN phosphatases ,PLANT growth regulation ,ABIOTIC stress ,CELLULAR signal transduction - Abstract
The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and F1-elated protein inase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that ype ne rotein hosphatase 1 (TOPP1) and its regulatory protein, nhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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20. Genetic dissection of winter barley seedling response to salt and osmotic stress.
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Xue, Wentao, Yan, Jun, Jiang, Yan, Zhan, Zhijie, Zhao, Gang, Tondelli, Alessandro, Cattivelli, Luigi, and Cheng, Jianping
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PSYCHOLOGICAL stress , *ABIOTIC stress , *ROOT growth , *PLANT genetics , *SALINITY - Abstract
Seedling establishment is a vulnerable stage in the crop life cycle which can be affected by different abiotic stresses. Drought and salinity are major environmental constraints worldwide, but few studies have genetically compared these two stresses on the same genetic material. In this study, the dynamic response of barley seedling growth to different levels of salt stress and PEG6000-mediated osmotic stress was evaluated in a European winter barley cultivar collection, in parallel with non-stress conditions. Salt and osmotic stress experiments produced different root-response curves. A final set of 56 phenotypic traits was subjected to genome-wide association mapping with 4885 gene-based SNP markers: 28 quantitative trait loci (QTL) were identified; 10 loci were found to be involved in saline conditions, whereas 20 loci were detected under osmotic stress. Four loci on chromosomes 1H, 5H and 6H were detected under more than one growth condition. One co-localized QTL was involved in both root and shoot growth only under salt stress. A set of potential candidate genes with putative pleiotropic effects on seedling growth under different conditions is proposed, based on their physical proximity to the QTL peak markers. To conclude, we found that a QTL controlling seedling growth under one abiotic stress can respond to another stress. QTL promoting faster growth under non-stress conditions were also identified under salt and/or osmotic stresses. These loci might be exploited in breeding programs to overcome environmental stresses at the initial seedling stage. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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21. Jasmonic Acid Signaling Pathway in Plants.
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Ruan, Jingjun, Zhou, Yuexia, Zhou, Meiliang, Yan, Jun, Khurshid, Muhammad, Weng, Wenfeng, Cheng, Jianping, and Zhang, Kaixuan
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JASMONIC acid ,PLANT growth ,PLANT development ,CELLULAR signal transduction ,ABIOTIC stress - Abstract
Jasmonic acid (JA) and its precursors and dervatives, referred as jasmonates (JAs) are important molecules in the regulation of many physiological processes in plant growth and development, and especially the mediation of plant responses to biotic and abiotic stresses. JAs biosynthesis, perception, transport, signal transduction and action have been extensively investigated. In this review, we will discuss the initiation of JA signaling with a focus on environmental signal perception and transduction, JA biosynthesis and metabolism, transport of signaling molecules (local transmission, vascular bundle transmission, and airborne transportation), and biological function (JA signal receptors, regulated transcription factors, and biological processes involved). [ABSTRACT FROM AUTHOR]
- Published
- 2019
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22. Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses
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Moon Young Ryu, Seok Keun Cho, Yourae Hong, Jinho Kim, Jong Hum Kim, Gu Min Kim, Yan-Jun Chen, Eva Knoch, Birger Lindberg Møller, Woo Taek Kim, Michael Foged Lyngkjær, and Seong Wook Yang
- Subjects
Barley ,Hordeum vulgare ,Ubiquitin proteasome system (UPS) ,Biotic stress ,Abiotic stress ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role in subjecting proteins to hydrolysis by the UPS. Recently, it has been demonstrated that a specific class of E3 ligases termed the U-box ligases are directly associated with the defense mechanisms against abiotic and biotic stresses in several plants. However, no studies on U-box E3 ligases have been performed in one of the important staple crops, barley. Results In this study, we identified 67 putative U-box E3 ligases from the barley genome and expressed sequence tags (ESTs). Similar to Arabidopsis and rice U-box E3 ligases, most of barley U-box E3 ligases possess evolutionary well-conserved domain organizations. Based on the domain compositions and arrangements, the barley U-box proteins were classified into eight different classes. Along with this new classification, we refined the previously reported classifications of U-box E3 ligase genes in Arabidopsis and rice. Furthermore, we investigated the expression profile of 67 U-box E3 ligase genes in response to drought stress and pathogen infection. We observed that many U-box E3 ligase genes were specifically up-and-down regulated by drought stress or by fungal infection, implying their possible roles of some U-box E3 ligase genes in the stress responses. Conclusion This study reports the classification of U-box E3 ligases in barley and their expression profiles against drought stress and pathogen infection. Therefore, the classification and expression profiling of barley U-box genes can be used as a platform to functionally define the stress-related E3 ligases in barley.
- Published
- 2019
- Full Text
- View/download PDF
23. Genome-wide identification and expression analysis of the superoxide dismutase (SOD) gene family in Salvia miltiorrhiza.
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Han, Li-min, Hua, Wen-ping, Cao, Xiao-yan, Yan, Jun-an, Chen, Chen, and Wang, Zhe-zhi
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SALVIA miltiorrhiza , *SUPEROXIDE dismutase , *GENE families , *CEREBROVASCULAR disease , *ABIOTIC stress - Abstract
• Three SmCSDs , two SmFSDs , and three SmMSDs were identified from Salvia miltiorrhiza. • SmSODs showed diverse response to chilling, salinity, copper, drought, and hormones. • The 31 types of potential TFs regulating the SmSODs were predicted and analyzed. Adverse environmental conditions, such as salinity, cold, drought, heavy metals, and pathogens affect the yield and quality of Salvia miltiorrhiza , a well-known medicinal plant used for the treatment of cardiovascular and cerebrovascular diseases. Superoxide dismutase (SOD), a key enzyme of antioxidant system in plants, plays a vital role in protecting plants against various biotic and abiotic stresses via scavenging the reactive oxygen species produced by organisms. However, little is known about the SOD gene family in S. miltiorrhiza. In this study, eight SOD genes, including three Cu/Zn-SODs , two Fe-SODs and three Mn-SODs , were identified in the S. miltiorrhiza genome. Their gene structures, promoters, protein features, phylogenetic relationships, and expression profiles were comprehensively investigated. Gene structure analysis implied that most SmSODs have different introns/exons distrbution patterns. Many cis -elements related to different stress responses or plant hormones were found in the promoter of each SmSOD. Expression profile analysis indicated that SmSODs exhibited diverse responses to cold, salt, drought, heavy metal, and plant hormones. Additionally, 31 types of TFs regulating SmSODs were predicted and analyzed. These findings provided valuable information for further researches on the functions and applications of SmSODs in S. miltiorrhiza growth and adaptation to stress. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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