8 results on '"Man, Suqin"'
Search Results
2. Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples
- Author
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Zheng, Lulu, Fu, Yongfeng, Jiang, Xiran, Man, Suqin, Ran, Wei, Feng, Meng, Liu, Sixiu, Cheng, Xunjia, and Sui, Guodong
- Published
- 2015
- Full Text
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3. Evaluation of the C-Terminal Fragment of Entamoeba histolytica Gal/GalNAc Lectin Intermediate Subunit as a Vaccine Candidate against Amebic Liver Abscess.
- Author
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Min, Xiangyang, Feng, Meng, Guan, Yue, Man, Suqin, Fu, Yongfeng, Cheng, Xunjia, and Tachibana, Hiroshi
- Subjects
ENTAMOEBA histolytica ,LECTINS ,VACCINE effectiveness ,AMEBIC liver abscess ,AMEBIASIS ,IMMUNIZATION - Abstract
Background: Entamoeba histolytica is an intestinal protozoan parasite that causes amoebiasis, including amebic dysentery and liver abscesses. E. histolytica invades host tissues by adhering onto cells and phagocytosing them depending on the adaptation and expression of pathogenic factors, including Gal/GalNAc lectin. We have previously reported that E. histolytica possesses multiple CXXC sequence motifs, with the intermediate subunit of Gal/GalNAc lectin (i.e., Igl) as a key factor affecting the amoeba's pathogenicity. The present work showed the effect of immunization with recombinant Igl on amebic liver abscess formation and the corresponding immunological properties. Methodology/Principal Findings: A prokaryotic expression system was used to prepare the full-length Igl and the N-terminal, middle, and C-terminal fragments (C-Igl) of Igl. Vaccine efficacy was assessed by challenging hamsters with an intrahepatic injection of E. histolytica trophozoites. Hamsters intramuscularly immunized with full-length Igl and C-Igl were found to be 92% and 96% immune to liver abscess formation, respectively. Immune-response evaluation revealed that C-Igl can generate significant humoral immune responses, with high levels of antibodies in sera from immunized hamsters inhibiting 80% of trophozoites adherence to mammalian cells and inducing 80% more complement-mediated lysis of trophozoites compared with the control. C-Igl was further assessed for its cellular response by cytokine-gene qPCR analysis. The productions of IL-4 (8.4-fold) and IL-10 (2-fold) in the spleen cells of immunized hamsters were enhanced after in vitro stimulation. IL-4 expression was also supported by increased programmed cell death 1 ligand 1 gene. Conclusions/Significance: Immunobiochemical characterization strongly suggests the potential of recombinant Igl, especially the C-terminal fragment, as a vaccine candidate against amoebiasis. Moreover, protection through Th2-cell participation enabled effective humoral immunity against amebic liver abscesses. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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4. Negative Regulation of Schistosoma japonicum Egg-Induced Liver Fibrosis by Natural Killer Cells.
- Author
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Hou, Xin, Yu, Fazhi, Man, Suqin, Huang, Dake, Zhang, Yuxia, Liu, Miao, Ren, Cuiping, and Shen, Jijia
- Subjects
HEPATIC fibrosis ,KILLER cells ,SCHISTOSOMA japonicum ,LIVER cells ,DEVELOPING countries - Abstract
The role of natural killer (NK) cells in infection-induced liver fibrosis remains obscure. In this study, we elucidated the effect of NK cells on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. Liver fibrosis was induced by infecting C57BL/6 mice with 18–20 cercariae of S. japonicum. Anti-ASGM1 antibody was used to deplete NK cells. Toll-like receptor 3 ligand, polyinosinic-polycytidylic acid (poly I∶C) was used to enhance the activation of NK cells. Results showed that NK cells were accumulated and activated after S. japonicum infection, as evidenced by the elevation of CD69 expression and IFN-γ production. Depletion of NK cells markedly enhanced S. japonicum egg-induced liver fibrosis. Administration of poly I∶C further activated NK cells to produce IFN-γ and attenuated S. japonicum egg-induced liver fibrosis. The observed protective effect of poly I∶C on liver fibrosis was diminished through depletion of NK cells. Disruption of IFN-γ gene enhanced liver fibrosis and partially abolished the suppression of liver fibrosis by poly I∶C. Moreover, expression of retinoic acid early inducible 1 (RAE 1), the NKG2D ligand, was detectable at high levels on activated hepatic stellate cells derived from S. japonicum-infected mice, which made them more susceptible to hepatic NK cell killing. In conclusion, our findings suggest that the activated NK cells in the liver after S. japonicum infection negatively regulate egg-induced liver fibrosis via producing IFN-γ, and killing activated stellate cells. Author Summary: Schistosomiasis continues to be a major public health problem in the developing world. Parasite egg-induced liver fibrosis is the principal cause of morbidity and mortality in human infected with schistosoma. Thus, elucidating the mechanisms that restrict tissue fibrosis may lead to more effective strategies for immunological intervention in this and a variety of chronic diseases. NK cells have been demonstrated to play an important role in suppressing carbon tetrachloride (CCl
4 )-induced liver fibrosis. However, little is known about the role of NK cells in an infection-based model of fibrosis. In the current study, we determined, for the first time, the role of NK cells in S. japonicum egg-induced liver fibrosis. Our findings suggest that the activated NK cells in the liver after S. japonicum infection negatively regulate egg-induced liver fibrosis via producing IFN-γ, and killing activated stellate cells. These results further our understanding of the innate immune cells that regulate the development of S. japonicum-induced fibrosis and aid in the development of potential strategies to enhance immunity against this and other chronic inflammatory diseases of the liver where fibrosis is a common feature. [ABSTRACT FROM AUTHOR]- Published
- 2012
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5. Phylogeographic analysis of severe fever with thrombocytopenia syndrome virus from Zhoushan Islands, China: implication for transmission across the ocean.
- Author
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Fu, Yongfeng, Li, Shibo, Zhang, Zhao, Man, Suqin, Li, Xueping, Zhang, Wenhong, Zhang, Chiyu, and Cheng, Xunjia
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- 2016
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6. Polyinosinic–polycytidylic acid attenuates hepatic fibrosis in C57BL/6 mice with Schistosoma japonicum infection
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Hou, Xin, Yu, Fazhi, Man, Suqin, Huang, Dake, Zhang, Yuxia, Liu, Miao, Ren, Cuiping, and Shen, Jijia
- Subjects
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LIVER diseases , *SCHISTOSOMA japonicum , *LABORATORY mice , *INOSINE , *MORTALITY , *GRANULOMA - Abstract
Abstract: The development of hepatic fibrosis is the principal cause of morbidity and mortality in human beings infected with schistosoma. In this study, we investigated the effect of polyinosinic–polycytidylic acid (poly I:C) on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. S. japonicum cercariae infected mice were injected with poly I:C at the onset of egg granuloma formation (early phase poly I:C treatment) or after the formation of liver fibrosis (late phase poly I:C treatment). Our results showed that both early and late phase poly I:C treatment significantly reduced collagen deposition and hepatic stellate cell activation in the liver. Poly I:C is one of the most effective adjuvants for Th1 type responses, and its protective effect on liver fibrosis was accompanied by increased IFN-α, IFN-β, IFN-γ, IL-12, TNF-α, and IL-10 mRNA expression, and decreased IL-4 and IL-5 mRNA expression. Moreover, poly I:C injection also enhanced the mRNA expression of natural killer group 2 member D (NKG2D) and tumor necrosis factor related apoptosis-inducing ligand (TRAIL). Therefore, it is indicated that poly I:C can significantly attenuate S. japonicum egg-induced hepatic fibrosis, which may be partly dependent on the increased Th1 response and decreased Th2 response. [Copyright &y& Elsevier]
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- 2012
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7. Evaluation of a Major Surface Antigen of Babesia microti Merozoites as a Vaccine Candidate against Babesia Infection.
- Author
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Man S, Fu Y, Guan Y, Feng M, Qiao K, Li X, Gao H, and Cheng X
- Abstract
Babesia species are tick-borne intraerythrocytic protozoa that cause babesiosis in humans worldwide. No vaccine has yet proven effective against Babesia infection. Surface antigens of merozoites are involved in the invasion of erythrocytes by Babesia . Surface antigens may be presented by both babesial sporozoites and merozoites and provide a general target for antibody-mediated inhibition of erythrocyte invasion. Here we evaluated a major surface antigen of B. microti merozoites, BMSA, as a potential vaccine to prevent babesiosis. Our data indicated that bmsa is transcribed during different phases, including ring form, amoeboid form, and merozoites, and that its expression is significantly increased in mature merozoites. The protein was found to be located in the membrane of B. microti and in the cytoplasm of infected erythrocytes. The immune response induced by BMSA had a significant inhibitory effect on parasite invasion of the host erythrocytes (83.3% inhibition of invasion) and parasite growth in vivo . The levels of parasitemia significantly decreased after BMSA vaccination when mice were infected with babesia parasite. Importantly, protective immunity was significantly related to the upregulation of the Th17 cytokine interleukin-17, the Th1 cytokine interleukin-12p70 and the Th2 cytokines, such as interleukin-4, -6, and -10. Ingenuity Pathway Analysis indicated that interleukin-17 facilitated the secretion of Th2 cytokines, such as interleukin-10, -4, and -6, thereby inducing a predominately Th2 protective immune response and promoting the expression a high level of special IgG1 against Babesia infection. Further, an anti-BMSA monoclonal antibody successfully protected NOD/SCID mice from a challenge with B. microti . Taken together, our results indicated that BMSA induces a protective immune response against Babesia infection and may serve as a potential vaccine.
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- 2017
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8. Artemether Exhibits Amoebicidal Activity against Acanthamoeba castellanii through Inhibition of the Serine Biosynthesis Pathway.
- Author
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Deng Y, Ran W, Man S, Li X, Gao H, Tang W, Tachibana H, and Cheng X
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- Acanthamoeba Keratitis drug therapy, Acanthamoeba Keratitis metabolism, Acanthamoeba Keratitis parasitology, Acanthamoeba castellanii metabolism, Amebiasis drug therapy, Amebiasis metabolism, Amebiasis parasitology, Animals, Apoptosis drug effects, Artemether, Cell Proliferation drug effects, Encephalitis drug therapy, Encephalitis metabolism, Encephalitis parasitology, Phosphoglycerate Dehydrogenase metabolism, Proteomics methods, Transaminases metabolism, Trophozoites parasitology, Acanthamoeba castellanii drug effects, Amebicides pharmacology, Antimalarials pharmacology, Artemisinins pharmacology, Biosynthetic Pathways drug effects, Serine metabolism
- Abstract
Acanthamoeba sp. parasites are the causative agents of Acanthamoeba keratitis, fatal granulomatous amoebic encephalitis, and cutaneous infections. However, there are currently no effective drugs for these organisms. Here, we evaluated the activity of the antimalarial agent artemether against Acanthamoeba castellanii trophozoites and identified potential targets of this agent through a proteomic approach. Artemether exhibited in vitro amoebicidal activity in a time- and dose-dependent manner and induced ultrastructural modification and cell apoptosis. The iTRAQ quantitative proteomic analysis identified 707 proteins that were differentially expressed after artemether treatment. We focused on phosphoglycerate dehydrogenase and phosphoserine aminotransferase in the serine biosynthesis pathway because of their importance to the growth and proliferation of protozoan and cancer cells. The expression of these proteins in Acanthamoeba was validated using quantitative real-time PCR and Western blotting after artemether treatment. The changes in the expression levels of phosphoserine aminotransferase were consistent with those of phosphoglycerate dehydrogenase. Therefore, the downregulation of phosphoserine aminotransferase may be due to the downregulation of phosphoglycerate dehydrogenase. Furthermore, exogenous serine might antagonize the activity of artemether against Acanthamoeba trophozoites. These results indicate that the serine biosynthesis pathway is important to amoeba survival and that targeting these enzymes would improve the treatment of Acanthamoeba infections. Artemether may be used as a phosphoglycerate dehydrogenase inhibitor to control or block Acanthamoeba infections., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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