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Conventionallymanaged citrus orchards can bemodified to incorporate advanced horticultural practices such as higher plant density and efficient water and fertilizer application known as open hydroponics system (OHS) to increase productivity under Huanglongbing (HLB) endemic conditions. A field studywas conducted from2013 to 2018 to evaluate the effect of an OHS on "Ray Ruby" grapefruit (RR) production under HLB-endemic conditions. We tested a combination of different rootstocks [Sour orange (RR/SO) and US-897 (RR/897)], tree planting densities [standard (STD, 358 trees per ha) and high density staggered (HDS, 953 trees per ha)], fertilization methods (dry granular--dry and fertigation--fert), and irrigation systems (double driplines--DD and microsprinkler--MS), arranged in five treatments: RR/SO_STD_dry_MS, RR/SO_HDS_fert_DD, RR/897_HDS_fert_MS, RR/897_HDS_fert_DD, and RR/SO_HDS_fert_MS. All trees were infected by Candidatus Liberibacter asiaticus five years after planting. Trunk diameter and canopy volume increased over time and were higher under RR/SO_STD_dry_MS compared to other treatments. Total fruit number increased in 2016/17 compared to other seasons; however, 65% of fruit were classified as small (<100 mm). Fruit produced under RR/897_HDS_fert_DD had the highest amount (79%) of adequate size fruit (100-117 mm) compared to other treatments. Fruit yield was similar for both rootstocks planted at HDS using DD andMS fertigation, and 67% higher than the standard treatment (RR/SO_STD_dry_MS). Soluble solid contents (SSC), titratable acidity, and SSC-to-titratable acidity ratio were not affected by the treatments. HDS planting resulted in higher fruit yield, irrespective of rootstock and irrigation system, representing an important advance in grapefruit production. Overall, our results demonstrated that production of grapefruit in high-density using OHS can be used by citrus growers who aim to make the best water and fertilizer management under HLB-endemic conditions. [ABSTRACT FROM AUTHOR]

Since the arrival of Huanglongbing (HLB) disease in Florida, several management approaches, includingmodification of orchard architecture design and nutritional therapy, have been explored. High-density plantings anticipate early economic returns from HLB-affected orchards. With no cure available for HLB, balanced nutrient application through soil and foliar spraying canmitigate the disease.A2-year study was conducted to investigate the effects of three grapefruit (Citrus paradisi) planting densities [single-row (300 and 440 trees per ha), and doublerow high-density (975 trees per ha)], two controlled-release fertilizer (CRF) blends, and foliarapplied micronutrients (FAM) (a blend of B, Mn, and Zn at 0, 1.5, 3, and 6 times the recommended rates) on grapefruit growth and fruit yield, physiological parameters, and foliar nutrient concentrations in an HLB-affected orchard. All the trees tested positive for HLB based on real-time quantitative polymerase chain reaction (qPCR) test. The highest planting density resulted in the lowest trunk diameter and canopy volume. Despite lower yield per tree in 2019- 20, 975 trees per ha planting induced the greatest fruit and solid yields per ha. Also, the fruit produced from 975 trees per ha planting tended to be acidic with the deposition ofmore soluble solids. Use of CRF with higher micronutrients increased canopy volume with the expense of reduced fruit number in 2019-20.FAMdid not affect cycle threshold (Ct) value and tree growth parameters. Fruit yield, photosynthesis rate, and stomatal conductance (gS) decreased, and all leaf nutrient concentrations except B increased in 2019-20 with all FAM rates tested. In conclusion, our study showed that high-density planting optimizes yield under HLB-endemic conditions. In addition, supplemental soil and foliar micronutrient application do not enhance yield of HLB-affected trees over a 2-year timeframe, warranting further research for confirmation of results. [ABSTRACT FROM AUTHOR]

Huanglongbing (HLB), or citrus greening disease, affects practically all fruitbearing trees in commercial citrus orchards in Florida with no cure identified yet. Highdensity plantings and enhanced nutritional programs such as application of controlledrelease fertilizer (CRF) with higher micronutrient levels can mitigate disease symptoms and extend the tree life span of sweet oranges (Citrus sinensis). The objective of this study was to evaluate the effects of tree planting density and application of CRF blends differing in N to K ratio and micronutrient content on grapefruit (Citrus paradisi) plant health, canopy volume, fruit yield, and fruit quality in an HLB-affected orchard. A study was conducted in Florida for two growing seasons (2017-18 and 2018-19) to evaluate the response of 'Ray Ruby' grapefruit on Kuharske citrange (Citrus sinensis 3 Poncirus trifoliata) to three planting densities (300, 440, and 975 trees per ha) and two CRF blends [12 nitrogen (N)-1.31 phosphorus (P)-7.47 potassium (K) and 16N-1.31P-16.6K] with different nutrient sources and composition. According to quantitative real-time polymerase chain reaction testing, all sampled trees tested positive for Candidatus Liberibacter asiaticus, the pathogen associated with HLB. Trees planted at 975 trees per ha had 33% lower canopy volume per tree but 160% greater fruit yield per hectare and 190% higher yield of solids compared with 300 trees per ha. Fruit produced in high-density planting (975 trees per ha) was 18% more acidic with higher soluble solid compared with low-density planting (300 trees per ha). The use of a CRF blend with higher amounts of micronutrients along with lower Kincreased canopy volume in both seasons and resulted in 24% and 29% reduction in fruit yield per hectare and yield of solids, respectively, in 2017-18. Our results indicate that high-density plantings increase fruit yield per area, and regardless of the N to K ratio, the use of CRF blends supplemented with micronutrients may not increase fruit yield in HLB-affected grapefruit. [ABSTRACT FROM AUTHOR]