1. MiR-601 Promotes Cell Proliferation of Human Glioblastoma Cells by Suppressing TINP1 Expression.
- Author
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Lei Chen, Shu Zhu, He Wang, Xingyi Pang, and Xuepeng Wang
- Subjects
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IN vitro studies , *REVERSE transcriptase polymerase chain reaction , *STAINS & staining (Microscopy) , *WESTERN immunoblotting , *ONE-way analysis of variance , *COLONY-forming units assay , *MICRORNA , *GLIOMAS , *GENE expression , *BIOINFORMATICS , *T-test (Statistics) , *CELL proliferation , *FLUORESCENT antibody technique , *DESCRIPTIVE statistics , *CELL lines , *TUMOR markers , *COLORIMETRY , *POLYMERASE chain reaction , *DATA analysis software - Abstract
Objective • It has been well documented that microRNAs (miRNAs) play essential roles in cancer initiation and development. In this study, we aimed to provide a better understanding of the mechanism of cell proliferation in glioblastoma (GBM). Methods • Levels of miR-601 expression were detected in GBM tissues and cells. The effects of miR-601 dysregulation on GBM cell proliferation by mean transit time (brain tissue blood flow) (MTT) assays, colony formation, anchorage-independent growth assay, bromodeoxyuridine (BrdU) labeling and immunofluorescence assay. Bioinformatics analysis, luciferase reporter system and Western blot assays were used to predict and confirm the target gene miR-601. Results • MiR-601 levels were identified as significantly up-regulated in GBM primary tumors and cell lines. Ectopic expression of miR-601 suppressed cell proliferation of GBM. Moreover, miR-601 showed its function by suppressing potential target TINP1 and TINP1 suppression reversed the effects of miR-601-in in U87MG GBM cells. Conclusion • Taken together, our results indicate that miR-601 promotes proliferation of GBM via inhibition of TINP1. [ABSTRACT FROM AUTHOR]
- Published
- 2022