6 results on '"Kida, Kouji"'
Search Results
2. Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein.
- Author
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Fukuma, Aiko, Fukushi, Shuetsu, Yoshikawa, Tomoki, Tani, Hideki, Taniguchi, Satoshi, Kurosu, Takeshi, Egawa, Kazutaka, Suda, Yuto, Singh, Harpal, Nomachi, Taro, Gokuden, Mutsuyo, Ando, Katsuyuki, Kida, Kouji, Kan, Miki, Kato, Nobuyuki, Yoshikawa, Akira, Kitamoto, Hiroaki, Sato, Yuko, Suzuki, Tadaki, and Hasegawa, Hideki
- Subjects
FEVER ,THROMBOCYTOPENIA ,ANTIGENS ,MONOCLONAL antibodies ,NUCLEOCAPSIDS ,PROTEINS - Abstract
Background: Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease with a high case fatality rate, and is caused by the SFTS virus (SFTSV). SFTS is endemic to China, South Korea, and Japan. The viral RNA level in sera of patients with SFTS is known to be strongly associated with outcomes. Virological SFTS diagnosis with high sensitivity and specificity are required in disease endemic areas. Methodology/Principal Findings: We generated novel monoclonal antibodies (MAbs) against the SFTSV nucleocapsid (N) protein and developed a sandwich antigen (Ag)-capture enzyme-linked immunosorbent assay (ELISA) for the detection of N protein of SFTSV using MAb and polyclonal antibody as capture and detection antibodies, respectively. The Ag-capture system was capable of detecting at least 350–1220 TCID
50 /100 μl/well from the culture supernatants of various SFTSV strains. The efficacy of the Ag-capture ELISA in SFTS diagnosis was evaluated using serum samples collected from patients suspected of having SFTS in Japan. All 24 serum samples (100%) containing high copy numbers of viral RNA (>105 copies/ml) showed a positive reaction in the Ag-capture ELISA, whereas 12 out of 15 serum samples (80%) containing low copy numbers of viral RNA (<105 copies/ml) showed a negative reaction in the Ag-capture ELISA. Among these Ag-capture ELISA-negative 12 samples, 9 (75%) were positive for IgG antibodies against SFTSV. Conclusions: The newly developed Ag-capture ELISA is useful for SFTS diagnosis in acute phase patients with high levels of viremia. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
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3. Japanese spotted fever with post-infectious encephalitis.
- Author
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Wada T, Mori H, Kida K, and Shindo K
- Abstract
Japanese spotted fever (JSF) is a rickettsial disease caused by Rickettsia japonica . To the best of our knowledge, there have only been five reported cases of JSF involving the central nervous system. A 74-year-old man was admitted after 1 week of fever and maculopapular rash. JSF was definitively diagnosed by PCR; however, the patient showed mental disturbance and abnormal behavior. After intravenous immunoglobulin, his mental state and behavior improved. The findings of cerebrospinal fluid analysis, electroencephalography, and
99 m TcHM-PAO single photon computed emission tomography suggested post-infectious encephalitis. JSF causes post-infectious encephalitis and early treatment is recommended., Competing Interests: The authors declare that there is no conflict of interest., (© 2022 The Authors.)- Published
- 2022
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4. A Case of Cat-to-Human Transmission of Severe Fever with Thrombocytopenia Syndrome Virus.
- Author
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Kida K, Matsuoka Y, Shimoda T, Matsuoka H, Yamada H, Saito T, Imataki O, Kadowaki N, Noguchi K, Maeda K, Mochizuki Y, and Kishimoto T
- Subjects
- Adult, Animals, Bunyaviridae Infections transmission, Bunyaviridae Infections veterinary, Cat Diseases virology, Cats, Female, Humans, Male, Occupational Diseases virology, Phlebovirus, Veterinarians, Bunyaviridae Infections diagnosis, Cat Diseases transmission, Occupational Diseases diagnosis
- Published
- 2019
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5. Extremely Low Genomic Diversity of Rickettsia japonica Distributed in Japan.
- Author
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Akter A, Ooka T, Gotoh Y, Yamamoto S, Fujita H, Terasoma F, Kida K, Taira M, Nakadouzono F, Gokuden M, Hirano M, Miyashiro M, Inari K, Shimazu Y, Tabara K, Toyoda A, Yoshimura D, Itoh T, Kitano T, Sato MP, Katsura K, Mondal SI, Ogura Y, Ando S, and Hayashi T
- Subjects
- Gene Expression Profiling, Humans, Japan, Phylogeny, Rickettsia classification, Rickettsia Infections genetics, Sequence Analysis, DNA, Genetic Variation, Genome, Bacterial, Rickettsia genetics, Rickettsia Infections microbiology
- Abstract
Rickettsiae are obligate intracellular bacteria that have small genomes as a result of reductive evolution. Many Rickettsia species of the spotted fever group (SFG) cause tick-borne diseases known as "spotted fevers". The life cycle of SFG rickettsiae is closely associated with that of the tick, which is generally thought to act as a bacterial vector and reservoir that maintains the bacterium through transstadial and transovarial transmission. Each SFG member is thought to have adapted to a specific tick species, thus restricting the bacterial distribution to a relatively limited geographic region. These unique features of SFG rickettsiae allow investigation of how the genomes of such biologically and ecologically specialized bacteria evolve after genome reduction and the types of population structures that are generated. Here, we performed a nationwide, high-resolution phylogenetic analysis of Rickettsia japonica, an etiological agent of Japanese spotted fever that is distributed in Japan and Korea. The comparison of complete or nearly complete sequences obtained from 31 R. japonica strains isolated from various sources in Japan over the past 30 years demonstrated an extremely low level of genomic diversity. In particular, only 34 single nucleotide polymorphisms were identified among the 27 strains of the major lineage containing all clinical isolates and tick isolates from the three tick species. Our data provide novel insights into the biology and genome evolution of R. japonica, including the possibilities of recent clonal expansion and a long generation time in nature due to the long dormant phase associated with tick life cycles., (© The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
- Published
- 2017
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6. Sensitive and specific PCR systems for detection of both Chinese and Japanese severe fever with thrombocytopenia syndrome virus strains and prediction of patient survival based on viral load.
- Author
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Yoshikawa T, Fukushi S, Tani H, Fukuma A, Taniguchi S, Toda S, Shimazu Y, Yano K, Morimitsu T, Ando K, Yoshikawa A, Kan M, Kato N, Motoya T, Kuzuguchi T, Nishino Y, Osako H, Yumisashi T, Kida K, Suzuki F, Takimoto H, Kitamoto H, Maeda K, Takahashi T, Yamagishi T, Oishi K, Morikawa S, Saijo M, and Shimojima M
- Subjects
- Blood virology, Humans, Japan, Phlebovirus genetics, Prognosis, RNA, Viral blood, Retrospective Studies, Bunyaviridae Infections diagnosis, Bunyaviridae Infections virology, Molecular Diagnostic Techniques methods, Phlebovirus isolation & purification, Real-Time Polymerase Chain Reaction methods, Reverse Transcriptase Polymerase Chain Reaction methods, Viral Load methods
- Abstract
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high case fatality risk and is caused by the SFTS virus (SFTSV). A retrospective study conducted after the first identification of an SFTS patient in Japan revealed that SFTS is endemic to the region, and the virus exists indigenously in Japan. Since the nucleotide sequence of Japanese SFTSV strains contains considerable differences compared with that of Chinese strains, there is an urgent need to establish a sensitive and specific method capable of detecting the Chinese and Japanese strains of SFTSV. A conventional one-step reverse transcription-PCR (RT-PCR) (cvPCR) method and a quantitative one-step RT-PCR (qPCR) method were developed to detect the SFTSV genome. Both cvPCR and qPCR detected a Chinese SFTSV strain. Forty-one of 108 Japanese patients suspected of having SFTS showed a positive reaction by cvPCR. The results from the samples of 108 Japanese patients determined by the qPCR method were in almost complete agreement with those determined by cvPCR. The analyses of the viral copy number level in the patient blood samples at the acute phase determined by qPCR in association with the patient outcome confirmed that the SFTSV RNA load in the blood of the nonsurviving patients was significantly higher than that of the surviving patients. Therefore, the cvPCR and qPCR methods developed in this study can provide a powerful means for diagnosing SFTS. In addition, the detection of the SFTSV genome level by qPCR in the blood of the patients at the acute phase may serve as an indicator to predict the outcome of SFTS., (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
- Published
- 2014
- Full Text
- View/download PDF
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