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Reactive cysteines often serve as functional sites on proteins and play important regulatory roles in various biological processes. A chemoproteomic method named "isoTOP‐ABPP" has enabled global profiling of cysteine reactivity in native systems and greatly increased our understanding on reactive cysteinome. This elegant method and its derived chemoproteomic technologies are now widely applied in profiling of reactive cysteinome and the associated post‐translational modifications, discovery of covalent ligands for undruggable targets as well as mechanistic investigation of various biological processes. In this review, we will briefly go over the history of isoTOP‐ABPP, summarize recent developments and applications of chemoproteomic technology in reactive cysteinome profiling, and discuss future directions in this field. [ABSTRACT FROM AUTHOR]

Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans. [ABSTRACT FROM AUTHOR]

Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans. [ABSTRACT FROM AUTHOR]