1. Identification, molecular characterization and analysis of the expression pattern of SoxF subgroup genes the Yellow River carp, Cyprinuscarpio.
- Author
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Liang, Tingting, Jia, Yongfang, Zhang, Ruihua, Du, Qiyan, and Chang, Zhongjie
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GENE expression ,NUCLEOTIDE sequencing ,CYPRINUS ,CYPRINIDAE ,DNA analysis - Abstract
Sox7 ,Sox17 andSox18 are the members of the Sry-related high-mobility group box family (SoxF ) of transcription factors.SoxF factors regulate endothelial cell fate as well as development and differentiation of blood cells and lymphatic vessels. There is very less information about the functions of these genes in fish. We obtained the full-length cDNA sequence ofSoxF genes includingSox7 ,Sox17 andSox18 inCyprinus carpio , whereSox7 andSox18 had two copies. The construction of a phylogenetic tree showed that these genes were homologous to the genes in other species. Chromosome synteny analysis indicated that the gene order ofSox7 andSox18 was highly conserved in fish. However, immense change in genomic sequences aroundSox17 had taken place. Numerous putative transcription factor binding sites were identified in the 5′flanking regions of SoxF genes which may be involved in the regulation of the nervous system, vascular epidermal differentiation and embryonic development. The expression levels ofSoxF genes were highest in gastrula, and was abundantly expressed in the adult brain. We investigated the expression levels ofSoxF genes in five specific parts of the brain. The expression levels ofSox7 andSox18 were highest in the mesencephalon, while the expression level ofSox17 was highest in the epencephalon. In carp, the expression patterns ofSoxF genes indicated a potential function of these genes in neurogenesis and in vascular development. These results provide new information for further studies on the potential functions ofSoxF genes in carp. [ABSTRACT FROM AUTHOR]- Published
- 2018
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