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A series of Ag-modified manganese-mullite (SmMn2O5) catalysts with different Ag contents (1, 3, and 6 wt.%) were prepared via a citric acid sol–gel method for catalytic soot oxidation. The catalysts were characterized by powder X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET), Raman spectroscopy, transmission electron microscopy (TEM), high-resolution transmission electron microscopy analysis (HRTEM), X-ray photoelectron spectroscopy (XPS), and H2 temperature-programmed reduction (H2-TPR). The soot oxidation activity of the mullite was significantly promoted by the addition of silver and affected by the loading amount of the metal. Herein, the influences of silver loading on the metal size distribution and its interactions with the mullite were studied. Based on these characterizations, a possible soot oxidation reaction mechanism was proposed for silver-modified SmMn2O5. [ABSTRACT FROM AUTHOR]

Cirrus thinning, as one kind of geoengineering approach, not only cools our planet but also enhances the amount of sunlight reaching the Earth's surface (brightening effect). This study delves into the brightening effect induced by cirrus thinning with a flexible seeding method. The thinning of cirrus clouds alone leads to a considerable globally averaged cooling effect (−2.46 W m−2), along with a notable globally averaged brightening effect (2.19 W m−2). Cirrus thinning also results in substantial reductions in the cloud radiative effects of the lower mixed-phase and liquid clouds. While these reductions counteract the cooling effect from cirrus clouds, they enhance the brightening effect from cirrus clouds. Consequently, the brightening effect caused by cirrus seeding (4.69 W m−2) is considerably stronger than its cooling effect (−1.21 W m−2). Furthermore, due to the more pronounced changes from the mixed-phase and liquid clouds at low and mid-latitudes, the cooling effect is primarily concentrated at high latitudes. In contrast, the brightening effect is stronger over most low- and mid-latitude regions. Overall, cirrus thinning could lead to a notable brightening effect, which can be leveraged to offset the dimming effect (the opposite of the brightening effect) of other geoengineering approaches. [ABSTRACT FROM AUTHOR]

An optical fiber sensor for the simultaneous measurement of microdisplacement and temperature based on balloon-shaped single-mode fibers cascaded with a fiber Bragg grating with two core-offset joints is proposed. The interference between the core mode and cladding mode is caused by the stimulation of the cladding mode by the core-offset joints' structure. The cladding of the core has a distinct refractive index, which causes optical path differences and interference. The balloon-shaped structure realizes mode selection by bending. As the displacement increases, the radius of the balloon-shaped interferometer changes, resulting in a change in the interference fringes of the interferometer, while the Bragg wavelength of the fiber grating remains unchanged. Temperature changes will cause the interference fringes of the interferometer and the Bragg wavelength of the fiber grating to shift. The proposed optical fiber sensor allows for the simultaneous measurement of microdisplacement and temperature. The results of the experiment indicate that the sensitivity of the interferometer to microdisplacement is 0.306 nm/µm in the sensing range of 0 to 200 μm and that the temperature sensitivity is 0.165 nm/°C, respectively. The proposed curvature sensor has the advantages of a compact structure, extensive spectrum of dynamic measurement, high sensitivity, and simple preparation, and has a wide range of potential applications in the fields of structural safety monitoring, aviation industry, and resource exploration. [ABSTRACT FROM AUTHOR]

Simple Summary: This study intended to understand the variation in egg and clutch mass among coexisting bird species at high altitudes. We investigated several life-history or ecological factors that could explain this variation. The results show that both egg and clutch mass were related to body mass across species. Contrary to the hypothesis of a trade-off between egg mass and clutch size, egg mass variation was not explained by clutch size when accounting for allometric effects. Clutch mass was found to be positively associated with parental care and negatively associated with predation rate. When considering clutch size and egg mass together, clutch size was significantly correlated with parental care, predation rate, and lifespan, while egg mass was only associated with development period. These results support the idea that reduced clutch size or mass is linked to a higher risk of predation, reduced parental care, and longer adult lifespan. Our findings suggest that clutch size has a greater impact on these factors compared to egg mass, possibly because smaller clutches represent a more significant reduction in energetic investment. This study has increased our understanding of how different factors influence the size of eggs and clutches in coexisting high-altitude bird species. The variation in egg and clutch mass in sympatric species at high altitudes is poorly understood, and the potential causes of variation are rarely investigated. This study aimed to describe the interspecific variation in avian egg and clutch mass among 22 sympatric bird species at an altitude of 3430 m. Our objective was to reduce potential confounding effects of biotic/abiotic factors and investigated hypotheses concerning allometry, clutch size, parental care, nest predation, and lifespan as possible correlates and explanations for the observed variation. Our findings indicated that both egg and clutch mass evolve with body mass across species. We found that egg mass variation was not explained by clutch size when controlling for allometric effects, which contrasts the "egg mass vs. clutch size trade-off" hypothesis. Additionally, we found that clutch mass was positively associated with parental care (reflected by development period) but negatively associated with predation rate. By substituting egg mass and clutch size into the models, we found that clutch size was significantly correlated with parental care, predation rate, and lifespan, while egg mass was only significantly associated with development period. Overall, these findings support life-history theories suggesting that reduced clutch size or mass is associated with a higher risk of predation, reduced parental care, but longer adult lifespan. Interestingly, our results indicate that clutch size has a greater influence on these factors compared to egg mass. This could be attributed to the fact that smaller clutch sizes result in a more notable decrease in energetic allocation, as they require a reduced effort in terms of offspring production, incubation, and feeding, as opposed to solely reducing egg size. These findings contribute to the growing evidence that life-history and ecological traits correlate with egg and clutch mass variation in sympatric species. However, further research is needed to explore the potential evolutionary causes underlying these patterns. [ABSTRACT FROM AUTHOR]

A continuous loading experiment of a two-dimensional granular system was carried out and the experimental data were obtained by digital image correlation (DIC). The deformation field of the granular system and the changing laws of the deflection angle and coordination number of the granules on force chains with time were obtained. Based on the granule element method, the quantitative calculation of contact force was realized, and the internal force chains of the granular system were identified. The effects of contact force between granules and mechanical parameters on the evolution of force chains in a two-dimensional granular system under line loads were analyzed. The formation, evolution, and reconstruction of force chains in a granular system during loading, as well as the influence of the force chain network evolution on the macroscopic mechanical properties of granules were discussed. The experimental results indicated that the evolution of force chains was directly related to the number, geometric properties, and permutation distribution of granules in direct contact with the external load. [ABSTRACT FROM AUTHOR]

The Split Vehicle Routing Problem with Simultaneous Delivery and Pickup (SVRPSDP) consists of two subproblems, i.e., the Vehicle Routing Problem with Simultaneous Delivery and Pickup (VRPSDP) and the Split Delivery Vehicle Routing Problem (SDVRP). Compared to the subproblems, SVRPSDP is much closer to reality. However, some realistic factors are still ignored in SVRPSDP. For example, the shipments are integrated and cannot be infinitely subdivided. Hence, this paper investigates the Granularity-based Split Vehicle Routing Problem with Simultaneous Delivery and Pickup (GSVRPSDP). The characteristics of GSVRPSDP are that the demands of customers are split into individual shipments and both the volume and weight of each shipment are considered. In order to solve GSVRPSDP efficiently, a Genetic-Simulated hybrid algorithm (GA-SA) is proposed, in which Simulated Annealing (SA) is inserted into the Genetic Algorithm (GA) framework to improve the global search abilities of individuals. The experimental results indicate that GA-SA can achieve lower total costs of routes compared to the traditional meta-algorithms, such as GA, SA and Particle Swarm Optimization (PSO), with a reduction of more than 10%. In the further analysis, the space utilization and capacity utilization of vehicles are calculated, which achieve 86.1% and 88.9%, respectively. These values are much higher than those achieved by GA (71.2% and 74.8%, respectively) and PSO (60.9% and 65.7%, respectively), further confirming the effectiveness of GA-SA. And the superiority of simultaneous delivery and pickup is proved by comparing with separate delivery and pickup. Specifically, the costs of separate delivery and pickup are more than 80% higher than that of simultaneous delivery and pickup. [ABSTRACT FROM AUTHOR]

Although the literature has shown that generalized reciprocity and negative reciprocity as exchange norms can significantly influence employees' outcomes, knowledge about how and when the two types of norms influence employees' well-being is limited. Based on social exchange theory and self-determination theory, we built and investigated a model by conducting a large questionnaire survey with 551 employees and managers. The results of the structural equation model supported our hypotheses. First, generalized reciprocity is positively related to well-being, and negative reciprocity is negatively related to well-being. Both intrinsic motivation and perceived organizational obstruction can meditate roles in the above relationships. Moreover, strength use can enhance the relationship between generalized reciprocity and intrinsic motivation, and it can also weaken the relationship between negative reciprocity and perceived organizational obstruction. Our research represents a significant step towards better understanding the work-related implications of imbalanced reciprocity, highlighting the destructive influence of negative reciprocity on employees' well-being. [ABSTRACT FROM AUTHOR]

Nitraria sibirica is a shrub that can survive in extreme drought environments. The auxin-response factors (ARFs) are a class of transcription factors that are widely involved in plant growth and development, as well as in the regulation of stress resistance. However, the genome-wide identification of the ARF gene family and its responses to environmental stresses, especially drought stress, in N. sibirica has not yet been reported. Here, we identified a total of 12 ARF genes in the genome of N. sibirica, which were distributed over 10 chromosomes and divided into three clades. Intragenome synteny analysis revealed one collinear gene pair in the ARF gene family, i.e., NsARF9a and NsARF9b. Cis-acting element analysis showed that multiple hormones and stress-responsive cis-acting elements were found in the promoters of NsARFs, suggesting that NsARFs may be involved in multiple biological processes. Quantitative real-time PCR (qRT-PCR) showed that many NsARFs had tissue-specific expression patterns, with the highest expression of NsARF16 in the seedlings of N. sibirica. In addition, most of the NsARFs that were upregulated under drought were independent of endogenous ABA biosynthesis, whereas the response of NsARF5 and NsARF7a to drought was disrupted by the ABA-biosynthesis inhibitor fluridone. These studies provide a basis for further research into how NsARFs in N. sibirica respond to hormonal signaling and environmental stresses. [ABSTRACT FROM AUTHOR]

Essentially, the solution to an attribute reduction problem can be viewed as a reduct searching process. Currently, among various searching strategies, meta-heuristic searching has received extensive attention. As a new emerging meta-heuristic approach, the forest optimization algorithm (FOA) is introduced to the problem solving of attribute reduction in this study. To further improve the classification performance of selected attributes in reduct, an ensemble framework is also developed: firstly, multiple reducts are obtained by FOA and data perturbation, and the structure of those multiple reducts is symmetrical, which indicates that no order exists among those reducts; secondly, multiple reducts are used to execute voting classification over testing samples. Finally, comprehensive experiments on over 20 UCI datasets clearly validated the effectiveness of our framework: it is not only beneficial to output reducts with superior classification accuracies and classification stabilities but also suitable for data pre-processing with noise. This improvement work we have performed makes the FOA obtain better benefits in the data processing of life, health, medical and other fields. [ABSTRACT FROM AUTHOR]

Proton exchange membrane (PEM) fuel cells using Pt-based materials as electrocatalysts have achieved a decent performance, represented by the launched Toyota Mirai vehicle. The ideal PEM fuel cells consume stored pure hydrogen and air. However, SO2, as a primary air contaminant, may be fed along with air at the cathode, leading to Pt site deactivation. Therefore, it is important to improve the SO2 tolerance of catalysts for the stability of the oxygen reduction reaction (ORR). In this work, we develop the Pt/C-TiO2 catalyst against SO2 poisoning during ORR. Impressively, the hybrid Pt/C-TiO2 catalyst with 20 mass % TiO2 shows the best ORR and anti-toxic performance: the kinetic current density of ORR is 20.5% higher and the degradation rate after poisoning is 50% lower than Pt/C. The interaction between Pt and TiO2 as well as the abundant hydroxyl groups on the surface of TiO2 are both revealed to account for the accelerated removal of poisonous SO2 on Pt surfaces. [ABSTRACT FROM AUTHOR]

Incorporating two organic ligands with different functionalities into a titanium-oxo cluster entity simultaneously can endow the material with their respective properties and provide synergistic performance enhancement, which is of great significance for enriching the structure and properties of titanium-oxo clusters (TOCs). However, the synthesis of such TOCs is highly challenging. In this work, we successfully synthesized a TBC4A-functionalized TOC, [Ti2(TBC4A)2(MeO)2] (Ti2; MeOH = methanol, TBC4A = tert-butylcalix[4]arene). By adjusting the solvent system, we successfully introduced 1,10-phenanthroline (Phen) and prepared TBC4A and Phen co-protected [Ti2(TBC4A)2(Phen)2] (Ti2-Phen). Moreover, when Phen was replaced with bulky 4,7-diphenyl-1,10-phenanthroline (Bphen), [Ti2(TBC4A)2(Bphen)2] (Ti2-Bphen), which is isostructural with Ti2-Phen, was obtained, demonstrating the generality of the synthetic method. Remarkably, Ti2-Phen demonstrates good stability and stronger light absorption, as well as superior photoelectric performance compared to Ti2. Density functional theory (DFT) calculations reveal that there exists ligand-to-core charge transfer (LCCT) in Ti2, while an unusual ligand-to-ligand charge transfer (LLCT) is present in Ti2-Phen, accompanied by partial LCCT. Therefore, the superior light absorption and photoelectric properties of Ti2-Phen are attributed to the existence of the unusual LLCT phenomenon. This study not only deeply explores the influence of Phen on the performance of the material but also provides a reference for the preparation of materials with excellent photoelectric performance. [ABSTRACT FROM AUTHOR]

With the overuse and misuse of antimicrobial drugs, antibacterial resistance is becoming a critical global health problem. New antibacterial agents are effective measures for overcoming the crisis of drug resistance. In this paper, a novel set of ciprofloxacin-indole/acetophenone hybrids was designed, synthesized, and structurally elucidated with the help of NMR and high-resolution mass spectrometry. The in vitro antibacterial activities of these hybrids against gram-positive and gram-negative pathogens, including four multidrug-resistant clinical isolates, were evaluated and compared with those of the parent drug ciprofloxacin (CIP). All the target compounds (MIC = 0.0625–32 μg/mL) exhibited excellent inhibitory activity against the strains tested. Among them, 3a (MIC = 0.25–8 μg/mL) showed comparable or slightly less potent activity than CIP. The most active hybrid, 8b (MIC = 0.0626–1 μg/mL), showed equal or higher activity than CIP. Moreover, compound 8b showed superior bactericidal capability to CIP, with undetectably low resistance frequencies. Furthermore, molecular docking studies conducted showed that 8b and CIP had a similar binding mode to DNA gyrase (Staphylocouccus aureus). Thus, hybrids 3a and 8b could act as a platform for further investigations. [ABSTRACT FROM AUTHOR]

Climate change has caused a widespread deduction in terrestrial water storage (TWS), leading to ocean water mass gains and sea level rises. A better understanding of how the land–sea water mass has been redistributed can help with the scientific response to climate change. However, there are few studies investigating the roles of the different physical processes involved in low-frequency land–sea water mass redistribution on a global scale. To address this issue, in this study, a comprehensive investigation was carried out with respect to the globally distributed key factors causing low-frequency ocean mass anomalies during the period 2004–2021. Global water mass redistribution data, derived from GRACE/GRACE-FO satellite gravity and surface wind and sea-surface temperature data from ERA5 reanalysis, were employed, and the empirical orthogonal function, maximum covariance analysis, and sea-level equation approaches were used. The results show that the long-term trend and decadal-like fluctuation are two major components of the low-frequency land–sea water mass redistribution. The wind-forcing dynamic processes significantly drive the anomalies near the North Indian Ocean, North Atlantic Ocean, South Pacific Ocean, and some marginal seas, where variance explanations range from 30% to 97%. After removing the ocean dynamics, the residual ocean mass anomaly is mostly explained by sea-level fingerprints (SLFs), especially in the open ocean. The 25th, 50th, and 75th percentiles of the SLF-explained variances in all ocean grids are 59%, 72%, and 82%, respectively. Some non-negligible noise, located in seismic zones, was also found, suggesting the misestimation of seafloor deformation resulting from earthquakes in the GRACE/GRACE-FO data processing. These findings may improve our understanding of the long-term anomalies in regional and global sea levels. [ABSTRACT FROM AUTHOR]

The spatial distribution and temporal variation of land surface sensible heat (SH) flux on the Tibetan Plateau (TP) for the period of 1981–2018 were studied using the simulation results from the Noah-MP land surface model. The simulated SH fluxes were also compared with the simulation results from the SEBS model and the results derived from 80 meteorological stations. It is found that, much larger annual mean SH fluxes occurred on the western and central TP compared with the eastern TP. Meanwhile, the inter-annual variations of SH fluxes on the central and western TP were larger than that on the eastern TP. The SEBS simulation showed much larger inter-annual variations than did the Noah-MP simulation across most of the TP. There was a trend of decrease in SH flux from the mid-1980s to the beginning of the 21st century in the Noah-MP simulations. Both Noah-MP and SEBS showed an increasing SH flux trend after this period of decrease. The increasing trend appeared on the eastern TP later than on the western and central TP. In the Noah-MP simulation, the western and central TP showed larger values of temperature difference between the ground surface and air (Ts–Ta) than did the eastern TP. Both mean Ts–Ta and wind speed decreased from the mid-1980s to approximately 2000, and then increased slightly. However, the Ts–Ta transition occurred later than that of wind speed. Changes in mean ground surface temperature (Ts) were the main cause of the decreasing and increasing trends in SH flux on the TP. Meanwhile, changes in wind speed contributed substantially to the decreasing trend in SH flux before 1998. [ABSTRACT FROM AUTHOR]

Using bulk formulas, two-year platform (fastened to the seabed) hourly observations from 2016 to 2017 in the East China Sea (121.6° E, 32.4° N) are used to investigate the role of the tide-induced surface elevation in changing the fixed observational height and modifying the momentum and air-sea turbulent heat fluxes. The semidiurnal tide-dominated elevation anomalies ranging from −3.6 to 3.9 m change the fixed platform observational height. This change causes hourly differences in the wind stress and latent and sensible heat fluxes between estimates with and without considering surface elevation, with values ranging from −1.5 × 10−3 Nm−2, −10.2 Wm−2, and −3.6 Wm−2 to 2.2 × 10−3 Nm−2, 8.4 Wm−2, and 4.6 Wm−2, respectively. More significant differences occur during spring tides. The differences show weak dependence on the temperature, indicating weak seasonal variations. The mean (maximum) difference percentage relative to the mean magnitude is approximately 3.5% (7%), 1.5% (3%), and 1.5% (3%) for the wind stress and latent and sensible heat fluxes, respectively. The boundary layer stability (BLS) can convert from near-neutral conditions to stable and unstable states in response to tide-induced changes in the observational height, with a probability of occurrence of 2%. Wind anomalies play dominant roles in determining the hourly anomalies of the latent heat flux, regardless of the state of the BLS. Extreme cases, including the cold air outbreak in 2016, tropical cyclones Meranti in 2016, and Ampil in 2018, are also examined. This study will facilitate future observation-reanalysis comparisons in the studied coastal region where ocean–atmosphere-land interactive processes are significant. [ABSTRACT FROM AUTHOR]

Lytic polysaccharide monooxygenases (LPMOs) have the potential to improve recalcitrant polysaccharide hydrolysis by the oxidizing cleavage of glycosidic bond. Streptomyces species are major chitin decomposers in soil ecological environments and encode multiple lpmo genes. In this study, we demonstrated that transcription of the lpmo gene, Sclpmo10G, in the Streptomyces coelicolor A3(2) (ScA3(2)) strain is strongly induced by chitin. The ScLPMO10G protein was further expressed in Escherichia coli and characterized in vitro. The ScLPMO10G protein showed oxidation activity towards chitin. Chitinase synergy experiments demonstrated that the addition of ScLPMO10G resulted in a substantial in vitro increase in the reducing sugar levels. Moreover, in vivo the LPMO-overexpressing strain ScΔLPMO10G(+) showed stronger chitin-degrading ability than the wild-type, leading to a 2.97-fold increase in reducing sugar level following chitin degradation. The total chitinase activity of ScΔLPMO10G(+) was 1.5-fold higher than that of ScA3(2). In summary, ScLPMO10G may play a role in chitin biodegradation in S. coelicolor, which could have potential applications in biorefineries. [ABSTRACT FROM AUTHOR]

In the theory of cosmology, de Sitter space is the symmetrical model of accelerated expansions of the universe. It is derived from the solution of the Einstein field equation, which has a positive cosmological constant. In this paper, we define the evolutes and focal surfaces of timelike Sabban curves in de Sitter space. We find that de Sitter focal surfaces can be regarded as caustics and de Sitter evolutes corresponding to the locus of the polar vectors of osculating de Sitter subspaces. By using singularity theory, we classify the singularities of the de Sitter focal surfaces and de Sitter evolutes and show that there is a close relationship between a new geometric invariant and the types of singularities. Moreover, the Legendrian dual relationships between the hyperbolic tangent indicatrix of timelike Sabban curves and the focal surfaces are given. Finally, we provide an example to illustrate our main results. [ABSTRACT FROM AUTHOR]

Simple Summary: Corn stover pellets are of poor quality (regarding physical and mechanical properties), and wet soybean dregs are viscous, which may improve the pelleting process. Furthermore, the soybean dregs have high nutritional value, which may increase the quality of feed. The relaxed density, dimensional stability coefficient and hardness of feed pellets were measured and analyzed in this study to clarify the influence mechanism of wet-fermented soybean dregs on the pelleting of corn stover. The results showed that fermented soybean dregs had a significant effect on corn stover pelleting. The comprehensive analysis showed that the addition of 5–10% fermented soybean dregs to corn stover improved the relaxed density, dimensional stability coefficient, and hardness of feed pellets by 10.76–23.51%, 7.32–15.74%, and 33.39–454.47%, respectively. The results could provide guidance on the research and development of high-quality forming technology and instruments using the mixture of corn stover and fermented soybean dregs and offer a new route for the utilization of high-moisture materials. Pelleting experiments were carried out in this study to clarify the influence mechanism of wet-fermented soybean dregs on the forming of corn stover. The effects of addition of water or wet fermented soybean dregs on the forming quality of corn stover were comparatively studied under different corn stover particle sizes and compression displacement. The fermented soybean dregs significantly affected the relaxed density, dimensional stability coefficient, and hardness of feed pellets. The relaxed density, dimensional stability coefficient, and hardness of feed pellets increased first and then decreased with the increase of fermented soybean dregs. The forming quality of corn stover added with fermented soybean dregs was higher than that of corn stover added with the same amount of water. The mechanism allowed soybean dregs to strengthen the bonding between corn stover particles and thus improved the quality of feed pellets. A certain amount of water was favorable for corn stover pelleting, but excessive water may decrease the quality of pellets. The comprehensive analysis showed that the addition of 5–10% fermented soybean dregs to the corn stover improved the relaxed density, dimensional stability coefficient, and hardness of feed pellets by 10.76–23.51%, 7.32–15.74%, and 33.39–454.47%, respectively. [ABSTRACT FROM AUTHOR]

To explore the drying characteristics of soybean dregs and a nondestructive moisture content test method, in this study, soybean dregs were dried with hot air (80 °C), the moisture content was measured using the drying method, water status was analyzed using low-field nuclear magnetic resonance (LF-NMR) and the moisture content prediction models were built and validated. The results revealed that the moisture contents of the soybean dregs were 0.57 and 0.01 g/g(w.b.), respectively, after drying for 5 and 7 h. The effective moisture diffusivity increased with the decrease in moisture content; it ranged from 5.27 × 10−9 to 6.96 × 10−8 m2·s−1. Soybean dregs contained bound water (T21), immobilized water (T22) and free water (T23 and T23'). With the proceeding of drying, all of the relaxation peaks shifted left until a new peak (T23') appeared; then, the structure of soybean dregs changed, and the relaxation peaks reformed, and the peak shifted left again. The peak area may predict the moisture content of soybean dregs, and the gray values of images predict the moisture contents mainly composed of free water or immobilized water. The results may provide a reference for drying of soybean dregs and a new moisture detection method. [ABSTRACT FROM AUTHOR]

CRISPR/Cas9-based cytosine base editors (CBEs) and adenine base editors (ABEs) can efficiently mediate C-to-T/G-to-A and A-to-G/T-to-C substitutions, respectively; however, achieving base transversions (C-to-G/C-to-A and A-to-T/A-to-C) is challenging and has been rarely studied in plants. Here, we constructed new plant C-to-G base editors (CGBEs) and new A-to-Y (T/C) base editors and explored their base editing characteristics in rice. First, we fused the highly active cytidine deaminase evoFENRY and the PAM-relaxed Cas9-nickase variant Cas9n-NG with rice and human uracil DNA N-glycosylase (rUNG and hUNG), respectively, to construct CGBE-rUNG and CGBE-hUNG vector tools. The analysis of five NG-PAM target sites showed that these CGBEs achieved C-to-G conversions with monoallelic editing efficiencies of up to 27.3% in T0 rice, with major byproducts being insertion/deletion mutations. Moreover, for the A-to-Y (C or T) editing test, we fused the highly active adenosine deaminase TadA8e and the Cas9-nickase variant SpGn (with NG-PAM) with Escherichia coli endonuclease V (EndoV) and human alkyladenine DNA glycosylase (hAAG), respectively, to generate ABE8e-EndoV and ABE8e-hAAG vectors. An assessment of five NG-PAM target sites showed that these two vectors could efficiently produce A-to-G substitutions in a narrow editing window; however, no A-to-Y editing was detected. Interestingly, the ABE8e-EndoV also generated precise small fragment deletions in the editing window from the 5′-deaminated A base to the SpGn cleavage site, suggesting its potential value in producing predictable small-fragment deletion mutations. Overall, we objectively evaluated the editing performance of CGBEs in rice, explored the possibility of A-to-Y editing, and developed a new ABE8e-EndoV tool, thus providing a valuable reference for improving and enriching base editing tools in plants. [ABSTRACT FROM AUTHOR]

Simple Summary: Breast cancer is a highly heterogeneous disease on the molecular level. The molecular subtype will be altered during metastasis and affect patient outcomes and treatments. Breast cancer is prone to lymph node metastasis. Our study aimed to investigate whether subtype conversion occurs during lymph node metastasis and its underlying mechanism. We confirmed that hormone receptors were down-regulated, while HER2 was up-regulated during lymph node metastasis, and macrophages played a significant role in the process, probably via MNX1. Targeting macrophages or MNX1 may provide new avenues for endocrine therapy and targeted treatment of breast cancer patients with lymph node metastasis. Background: The progression of tumors from less aggressive subtypes to more aggressive states during metastasis poses challenges for treatment strategies. Previous studies have revealed the molecular subtype conversion between primary and metastatic tumors in breast cancer (BC). However, the subtype conversion during lymph node metastasis (LNM) and the underlying mechanism remains unclear. Methods: We compared clinical subtypes in paired primary tumors and positive lymph nodes (PLNs) in BC patients and further validated them in the mouse model. Bioinformatics analysis and macrophage-conditioned medium treatment were performed to investigate the role of macrophages in subtype conversion. Results: During LNM, hormone receptors (HRs) were down-regulated, while HER2 was up-regulated, leading to the transformation of luminal A tumors towards luminal B tumors and from luminal B subtype towards HER2-enriched (HER2-E) subtype. The mouse model demonstrated the elevated levels of HER2 in PLN while retaining luminal characteristics. Among the various cells in the tumor microenvironment (TME), macrophages were the most clinically relevant in terms of prognosis. The treatment of a macrophage-conditioned medium further confirmed the downregulation of HR expression and upregulation of HER2 expression, inducing tamoxifen resistance. Through bioinformatics analysis, MNX1 was identified as a potential transcription factor governing the expression of HR and HER2. Conclusion: Our study revealed the HER2-E subtype conversion during LNM in BC. Macrophages were the crucial cell type in TME, inducing the downregulation of HR and upregulation of HER2, probably via MNX1. Targeting macrophages or MNX1 may provide new avenues for endocrine therapy and targeted treatment of BC patients with LNM. [ABSTRACT FROM AUTHOR]

We report a unique constant phase element (CPE) behavior ( 1 Z = Q 0 (j ω) α ) of the electrolyte–graphene interface with both Q 0 and α showing dependence on the gate voltage. The frequency response of the electrolyte–graphene interface was studied using electrochemical impedance spectroscopy (EIS). The result suggests that (1) the electrolyte–graphene interface should be characterized as a CPE (α < 1), rather than an ideal capacitor; and (2) both Q 0 and α show ambipolar dependence on the applied voltage. We speculate that the CPE behavior of the electrolyte–graphene interface arises from the charged impurities on the substrate and the defects in the graphene lattice, which could introduce inhomogeneity of local density of states (DOS). The low density of states of graphene makes α sensitive to these local DOS near the Dirac point, and thus showing dependence on the gate voltage. Measurement of the electrolyte–graphene interface capacitance based on multi-frequency capacitance-voltage (CV) profiling was demonstrated, and the extraction of the carrier mobility was performed. The study could lead to a more accurate understanding of the capacitive behavior of the electrolyte–graphene interface, which is instructive for the design and analysis of devices involving the electrolyte–graphene interface for nanoelectronics and bioelectronics applications. [ABSTRACT FROM AUTHOR]

Neuromorphic photonics devices based on phase change materials (PCMs) and silicon photonics technology have emerged as promising solutions for addressing the limitations of traditional spiking neural networks in terms of scalability, response delay, and energy consumption. In this review, we provide a comprehensive analysis of various PCMs used in neuromorphic devices, comparing their optical properties and discussing their applications. We explore materials such as GST (Ge2Sb2Te5), GeTe-Sb2Te3, GSST (Ge2Sb2Se4Te1), Sb2S3/Sb2Se3, Sc0.2Sb2Te3 (SST), and In2Se3, highlighting their advantages and challenges in terms of erasure power consumption, response rate, material lifetime, and on-chip insertion loss. By investigating the integration of different PCMs with silicon-based optoelectronics, this review aims to identify potential breakthroughs in computational performance and scalability of photonic spiking neural networks. Further research and development are essential to optimize these materials and overcome their limitations, paving the way for more efficient and high-performance photonic neuromorphic devices in artificial intelligence and high-performance computing applications. [ABSTRACT FROM AUTHOR]

Cervical cancer causes malignant tumors in females and threatens the physical and mental health of women. Current research shows that persistent infection of high-risk HPV is the main cause of cervical cancer. However, not all cervical cancer is caused by HPV infection, which may also be related to other factors, such as nutritional status and lifestyle. This study focuses on the effect of alcohol consumption on the methylation status of cervical cancer from the perspective of methyl donors. We established a mouse tumor-bearing model with cervical cancer SiHa cells, and at the same time, we cultured SiHa cells in vitro. Different concentrations of ethanol were administered to the model mice and SiHa cells. Then, we detected the levels of the methyl-donor folate and methionine and their metabolite homocysteine levels in mice serum, tumor tissues, and SiHa cells. Furthermore, we determined the expression of the members of the DNA methyltransferase family (DNMT1, DNMT3a, and DNMT3b) in tumor tissue by immunohistochemistry. qRT-PCR and Western blotting analysis were used to detect the mRNA and protein levels of members of the DNA methyltransferase family in cervical cancer SiHa cells. Our results show that the levels of the methyl donor (folate and methionine) decreased with the increase of ethanol concentration (p < 0.05), and the homocysteine level increased significantly (p < 0.05). In SiHa cells, the mRNA and protein levels of the DNMT family members and their receptors were significantly higher than those in the control group (p < 0.05). Collectively, these results suggest that ethanol could influence DNMT expression by inducing methyl donor consumption, thereby causing cervical cancer cells to exhibit genome-wide hypomethylation. [ABSTRACT FROM AUTHOR]

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the GPX gene family and its responses to environmental stresses, especially salt stress, in Nitraria sibirica, which is a shrub that can survive in saline environments, has not yet been reported. Here, we first report the genome-wide analysis of the GPX gene family in N. sibirica, leading to a total of seven NsGPX genes that are distributed on six of the twelve chromosomes. Phylogenetic analysis showed that NsGPX genes were grouped into four major groups (Group I-IV). Three types of cis-acting elements were identified in the NsGPX promoters, mainly related to hormones and stress response. The quantitative real-time PCR (qRT-PCR) analysis indicated that NsGPX1 and NsGPX3 were significantly up-regulated in stem and leaf, while NsGPX7 transcriptionally in root in response to salt stress. The current study identified a total seven NsGPX genes in N. sibirica via genome-wide analysis, and discovered that NsGPXs may play an important role in response to salt stress. Taken together, our findings provide a basis for further functional studies of NsGPX genes, especially in regarding to the resistance to salt stress of this halophyte plant N. sibirica, eventually aid in the discovery of new methods to restore overtly saline soil. [ABSTRACT FROM AUTHOR]

In this paper, the influence of calcium on coal gangue and fly ash geopolymer is explored, and the problem of low utilization of unburned coal gangue is analyzed and solved. The experiment took uncalcined coal gangue and fly ash as raw materials, and a regression model was developed with the response surface methodology. The independent variables were the CG content, alkali activator concentration, and Ca(OH)2 to NaOH ratio (CH/SH). The response target value was the coal gangue and fly-ash geopolymer compressive strength. The compressive strength tests and the regression model obtained by the response surface methodology showed that the coal gangue and fly ash geopolymer prepared with the content of uncalcined coal gangue is 30%, alkali activator content of 15%, and the value of CH/SH is 1.727 had a dense structure and better performance. The microscopic results demonstrated that the uncalcined coal gangue structure is destroyed under an alkali activator's action, and a dense microstructure is formed based on C(N)-A-S-H and C-S-H gel, which provides a reasonable basis for the preparation of geopolymers from the uncalcined coal gangue. [ABSTRACT FROM AUTHOR]

Simple Summary: The black-necked crane is the only crane species that breeds on the Qinghai–Tibetan plateau, and habitat destruction and loss are the major threats to it. The Shaluli Mountains, located in a biological hotspot, is an important breeding site for the central population of the black-necked crane, but its population size and distribution, as well as shifts in distribution under climatic change, remain unknown. Therefore, we conducted a field survey and modeled the breeding black-necked crane habitat with a species distribution model in the region. The results showed that the number of breeding black-necked cranes in the area was about 200, and they were mainly distributed in a gentle meadow and wetland along the lake. Climate change will increase the potentially suitable habitat area, with a trend of northwestward expansion and upward migration, as well as an increase in habitat suitability and connectivity. In addition, more conservation gaps will arise, but the conservation role of existing protected areas will not decline. Therefore, we suggest strengthening the long-term monitoring of black-necked cranes and wetland resources in this area. Climate change is affecting biodiversity by altering the geographical distribution range of species, and this effect is amplified in climate-sensitive areas. Studying the geographic distribution of flagship species in response to climate change is important for the long-term conservation of species and the maintenance of regional biodiversity. Therefore, we collected field survey records from 2016 to 2020 and conducted field surveys of black-necked cranes in the Shaluli Mountains (SLLMs) in May–June and August–October 2021; 103 breeding records were acquired totally, and the geographical distribution range under the current and four future climate scenarios was modeled with the MaxEnt model to predict the impact of climate change on its distribution and habitat quality. The results showed that 152 black-necked cranes were surveyed in seven counties of SLLMs in total; the estimated number of black-necked cranes in the entire SLLMs was about 200. The currently suitable habitat area is 27,122 km2, mainly distributed in gentle meadows and wetland habitats along the lake where the Annual Mean Temperature is −1 °C and the Mean Diurnal Range (16 °C) and Precipitation Seasonality (105) are comparatively large. Furthermore, the breeding range would expand to varying degrees under future climate scenarios and showed a migration trend toward the northwest and higher elevation. Besides, as time goes by, the habitat for black-necked cranes in SLLMs would become more homogeneous and more suitable. The conservation effectiveness of the existing reserve network would keep stable with climate change, although there are large conservation gaps between protected areas, and these gaps will gradually expand over time. Overall, this study provides a preliminary understanding of the population and distribution and predicts the future distribution of black-necked cranes in the SLLMs. It also demonstrates the importance of SLLMs for protecting the central population of black-necked cranes and maintaining regional biodiversity. Therefore, we recommend long-term monitoring and conservation of the black-necked crane population and wetland resources in the region. [ABSTRACT FROM AUTHOR]

Shading facilities are important technology to enable the adjustment of the indoor light and heat environment, and the control logic of the technology relies on data collected by sensors. The sensor position is generally arranged on the work surface, which is only suitable for single rooms with fixed locations. For open-plan offices or other large offices, more study of detailed designs for the sensor position is required. Therefore, various sensor locations for different spaces will be investigated. Based on existing research, the UDI2000 lux [50%] and UDI450–2000 lux [50%] are the key indices for measuring sensor location. The Entropy Weight method is used to determine the weight of each index, and the ideal point method (TOPSIS method) is used to select the best sensor location. Based on the results, recommendations are provided for different space scales, window-to-wall ratios, and building orientations of offices for shading control sensor location. [ABSTRACT FROM AUTHOR]

Urbanization can induce environmental changes such as the urban heat island effect, which in turn influence the terrestrial ecosystem. However, the effect of urbanization on the phenology of subtropical vegetation remains relatively unexplored. This study analyzed the changing trend of vegetation photosynthetic phenology in Dongting Lake basin, China, and its response to urbanization using nighttime light and chlorophyll fluorescence datasets. Our results indicated the start of the growing season (SOS) of vegetation in the study area was significantly advanced by 0.70 days per year, whereas the end of the growing season (EOS) was delayed by 0.24 days per year during 2000–2017. We found that urbanization promoted the SOS advance and EOS delay. With increasing urbanization intensity, the sensitivity of SOS to urbanization firstly increased then decreased, while the sensitivity of EOS to urbanization decreased with urbanization intensity. The climate sensitivity of vegetation phenology varied with urbanization intensity; urbanization induced an earlier SOS by increasing preseason minimum temperatures and a later EOS by increasing preseason precipitation. These findings improve our understanding of the vegetation phenology response to urbanization in subtropical regions and highlight the need to integrate human activities into future vegetation phenology models. [ABSTRACT FROM AUTHOR]

The ferroelectric domain surface charge dynamics after a cubic-to-tetragonal phase transition on the BaTiO3 single crystal (001) surface was directly measured through scanning probe microscopy. The captured surface potential distribution shows significant changes: the domain structures formed rapidly, but the surface potential on polarized c domain was unstable and reversed its sign after lengthy lapse; the high broad potential barrier burst at the corrugated a-c domain wall and continued to dissipate thereafter. The generation of polarization charges and the migration of surface screening charges in the surrounding environment take the main responsibility in the experiment. Furthermore, the a-c domain wall suffers large topological defects and polarity variation, resulting in domain wall broadening and stress changes. Thus, the a-c domain wall has excess energy and polarization change is inclined to assemble on it. The potential barrier decay with time after exposing to the surrounding environment also gave proof of the surface screening charge migration at surface. Thus, both domain and domain wall characteristics should be taken into account in ferroelectric application. [ABSTRACT FROM AUTHOR]

The IN738LC Ni-based superalloy strengthened by the coherent γ′-Ni3(Al,Ti) intermetallic compound is one of the most employed blade materials in gas turbine engines and IN738LC thin wall components without macro-cracks were fabricated by pulsed plasma arc additive manufacturing (PPAAM), which is more competitive when considering convenience and cost in comparison with other high-energy beam additive manufacturing technologies. The as-fabricated sample exhibited epitaxial growth columnar dendrites along the building direction with discrepant secondary arm spacing due to heat accumulation. A lot of fine γ′ particles with an average size of 81 nm and MC carbides were observed in the interdendritic region. Elemental segregation and γ–γ′ eutectic reaction were analyzed in detail and some MC carbides were confirmed in the reaction L + MC→γ + γ′. After standard heat treatment, bimodal distribution of γ′ phases, including coarse γ′ particles (385 nm, 42 vol.%) and fine γ′ particles (42 nm, 25 vol.%), was observed. The mechanism of microstructural evolution, phase formation, as well as cracking mechanisms were discussed. Microhardness and tensile tests were carried out to investigate the mechanical performance. The results show that both the as-fabricated and heat-treated samples exhibited a higher tensile strength but a slightly lower ductility compared with cast parts. [ABSTRACT FROM AUTHOR]

The coseismic slip on the main fault related to the 2017 Iran-Iraq Mw7.3 earthquake has been investigated by previous studies using DInSAR (differential interferometric synthetic aperture radar) ground deformation measurements. However, DInSAR observation is not sensitive to the ground deformation in the along-track (AT) direction. Therefore, only the one-dimensional (1D) DInSAR coseismic deformation field measurements, derived in the LOS (line-of-sight) direction of radar, was applied in source parameters estimation. To further improve the accuracy of the fault slip inversion, the 3D (three-dimensional) coseismic deformation fields were reconstructed in the first place, by a combined use of the DInSAR and MAI (multiple aperture InSAR) measurements. Subsequently, the LOS and 3D deformation data sets were used as the constraint respectively, to perform a two-step inversion scheme to find an optimal geometry and slip distribution on the main fault. The comparative analysis indicated that the 3D coseismic deformation data sets improved the inversion-accuracy by 30%. Besides, the fault invention results revealed a deep dislocation on a NNW trending fault (the strike is 352.63°) extending about 60 km, along the fault dips 14.76° to the ENE. The estimated seismic moment is 8.44 × 1019 Nm (Mw7.3), which is close to the solution provided by USGS (United States Geological Survey). The slip distributed at the depth between 12 and 18 km, and the peak slip of 6.53 m appears at the depth of 14.5 km left near the epicenter. Considering the geological structure in the earthquake region and fault source-parameters, it comes to a preliminary conclusion that the ZMFF (the Zagros Mountain Front fault) should be responsible for the earthquake. In general, this paper demonstrated the superiority of using the 3D coseismic deformation fields on source parameters estimation. [ABSTRACT FROM AUTHOR]

With the development of deep learning, researchers design deep network structures in order to extract rich high-level semantic information. Nowadays, most popular algorithms are designed based on the complexity of visible image features. However, compared with visible image features, infrared image features are more homogeneous, and the application of deep networks is prone to extracting redundant features. Therefore, it is important to prune the network layers where redundant features are extracted. Therefore, this paper proposes a pruning method for deep convolutional network based on heat map generation metrics. The 'network layer performance evaluation metrics' are obtained from the number of pixel activations in the heat map. The network layer with the lowest 'network layer performance evaluation metrics' is pruned. To address the problem that the simultaneous deletion of multiple structures may result in incorrect pruning, the Alternating training and self-pruning strategy is proposed. Using a cyclic process of pruning each model once and retraining the pruned model to reduce the incorrect pruning of network layers. The experimental results show that proposed method in this paper improved the performance of CSPDarknet, Darknet and Resnet. [ABSTRACT FROM AUTHOR]

Symmetry-based channel digital twin is a great technology which can reproduce the communication channel of real scenes for performance evaluation of the wireless sensor network (WSN) inside tower buildings, based on the ray tracing (RT) method and machine learning (ML) theories, a cluster-based channel model is proposed in this paper. Meanwhile, an improved k-means method, which considers the weight of different dimensions in the multipath component distance (MCD) is presented for clustering, which has better clustering performance over the sparsity-based algorithm and traditional k-means algorithm. Moreover, the channel parameters such as cluster delay and cluster power are also investigated. On this basis, the communication performance of WSN, i.e., bit error rate (BER) and channel capacity are derived and analyzed. The simulation and analysis results show that the cluster model based on the RT method can get approximately equivalent channel impulse response (CIR), and the BER of proposed model is consistent with the simulated one. These results can provide reference for the node layout and optimization of WSN inside tower buildings. [ABSTRACT FROM AUTHOR]

F-box genes play an important role in the growth and development of plants, but there are few studies on its role in a plant's response to abiotic stresses. In order to further study the functions of F-box genes in tomato (Solanum lycopersicum, Sl), a total of 139 F-box genes were identified in the whole genome of tomato using bioinformatics methods, and the basic information, transcript structure, conserved motif, cis-elements, chromosomal location, gene evolution, phylogenetic relationship, expression patterns and the expression under cold stress, drought stress, jasmonic acid (JA) treatment and salicylic acid (SA) treatment were analyzed. The results showed that SlFBX genes were distributed on 12 chromosomes of tomato and were prone to TD (tandem duplication) at the ends of chromosomes. WGD (whole genome duplication), TD, PD (proximal duplication) and TRD (transposed duplication) modes seem play an important role in the expansion and evolution of tomato SlFBX genes. The most recent divergence occurred 1.3042 million years ago, between SlFBX89 and SlFBX103. The cis-elements in SlFBX genes' promoter regions were mainly responded to phytohormone and abiotic stress. Expression analysis based on transcriptome data and qRT-PCR (Real-time quantitative PCR) analysis of SlFBX genes showed that most SlFBX genes were differentially expressed under abiotic stress. SlFBX24 was significantly up-regulated at 12 h under cold stress. This study reported the SlFBX gene family of tomato for the first time, providing a theoretical basis for the detailed study of SlFBX genes in the future, especially the function of SlFBX genes under abiotic stress. [ABSTRACT FROM AUTHOR]

Due to the unique adsorption and desorption characteristics of coal, coal reservoir permeability changes dynamically during coalbed methane (CBM) development. Coal reservoirs can be classified using a permeability dynamic characterization in different production stages. In the single-phase water flow stage, four demarcating pressures are defined based on the damage from the effective stress on reservoir permeability. Coal reservoirs are classified into vulnerable, alleviative, and invulnerable reservoirs. In the gas desorption stage, two demarcating pressures are used to quantitatively characterize the recovery properties of permeability based on the recovery effect of the matrix shrinkage on permeability, namely the rebound pressure (the pressure corresponding to the lowest permeability) and recovery pressure (the pressure when permeability returns to initial permeability). Coal reservoirs are further classified into recoverable and unrecoverable reservoirs. The physical properties and influencing factors of these demarcating pressures are analyzed. Twenty-six wells from the Shizhuangnan Block in the southern Qinshui Basin of China were examined as a case study, showing that there is a significant correspondence between coal reservoir types and CBM well gas production. This study is helpful for identifying geological conditions of coal reservoirs as well as the productivity potential of CBM wells. [ABSTRACT FROM AUTHOR]

Solar energy is the most clean renewable energy source and has good prospects for future sustainable development. Installation of solar photovoltaic (PV) systems on building rooftops has been the most widely applied method for using solar energy resources. In this study, we developed an approach to simulate the monthly and annual solar radiation on rooftops at an hourly time step to estimate the solar PV potential, based on rooftop feature retrieval from remote sensing images. The rooftop features included 2D rooftop outlines and 3D rooftop parameters retrieved from high-resolution remote sensing image data (obtained from Google Maps) and digital surface model (DSM, generated from the Pleiades satellite), respectively. We developed the building features calculation method for five rooftop types: flat rooftops, shed rooftops, hipped rooftops, gable rooftops and mansard rooftops. The parameters of the PV modules derived from the building features were then combined with solar radiation data to evaluate solar photovoltaic potential. The proposed method was applied in the Chao Yang District of Beijing, China. The results were that the number of rooftops available for PV systems was 743, the available rooftop area was 678,805 m2, and the annual PV electricity potential was 63.78 GWh/year in the study area, which has great solar PV potential. The method to perform precise calculation of specific rooftop solar PV potential developed in this study will guide the formulation of energy policy for solar PV in the future. [ABSTRACT FROM AUTHOR]