1. Impact of prematurity on LINE-1 promoter methylation.
- Author
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Barbosa Eleutério Dos Santos PV, Araújo Brasil A, Vieira Milone LT, Chalfun G, Alves de Oliveira Saide SC, Santos Salú MD, Genuíno de Oliveira MB, Robaina JR, Lima-Setta F, Rodrigues-Santos G, de Magalhães-Barbosa MC, Ledo Alves da Cunha AJ, and Prata-Barbosa A
- Abstract
Aim: Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature. Materials & methods: Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing. Results: Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns. Conclusion: This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.
- Published
- 2024
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