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• Foundations of experimental geothermic studies were significantly improved. • Advanced technique was applied to study the Volga-Ural oil and gas basin. • Field experiment included 7 cycles of temperature logging during 186 days of well shut-in. • Unique data on temperature and its gradient behavior after drilling were obtained. • A significant increase in heat flow with depth was established. • The necessity for new, special experimental estimations of heat flow is demonstrated. • Thermal core profiling is needed for estimation the detailed variations of heat flow. • One more prove that heat flow data obtained with common technique are unreliable. The paper describes the results of experimental geothermal studies of the formation surrounding Savitskaya-300 well (depth 3555 m) drilled in the Volga-Ural oil and gas basin (Orenburg region, Russia). The work was aimed, firstly, at obtaining geothermal data for basin modeling and, secondly, at improving the instrumental and methodological foundations of experimental studies. Continuous core profiling of the rock thermal conductivity, volumetric heat capacity, and thermal anisotropy was performed on all 2886 recovered core samples with the total length of 329.1 m. Additional measurements were conducted at fluid saturation and elevated temperatures. For non-cored intervals, the rock thermal properties were determined from well-logging data and via measurements on rock cuttings. The studied formation is characterized by high thermal heterogeneity at both the level of strata (horizons) and individual samples. Significant thermal anisotropy up to 1.50–1.66 in the lower part of the well has been observed. To obtain data on vertical variations of equilibrium temperature and its gradient, temperature logging was performed 7 times within the 2 to 186 days of well shut-in. New data on the recovery of temperature and its gradient after drilling are important for the practice of applied and fundamental geothermal surveys. A significant increase in heat flow with depth was established based on the results of temperature gradient and rock thermal conductivity determination for 34 depth intervals. The observed general vertical variation in the whole depth range of the well up to ∼3.3 km depth can be attributed to paleoclimatic effect in the Late Pleistocene-Holocene, while the sharp increase in heat flow at ∼3.3 km within Fransian sediments is possibly due to advective heat transfer by fluid filtration. The apparent heat flow was established to be 84.3 mW⋅m−2 in the lower section of the well, below 3.3 km. The value may be close to the steady-state heat flow value, which is 84% higher than the previous published average data for the studied area. [ABSTRACT FROM AUTHOR]