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Copper pollution has attracted global environmental concern. Widespread Cu pollution results in excessive Cu accumulation in human. Epidemiological studies and animal experiments revealed that Cu exposure might have reproductive toxicity. Cuproptosis is a recently reported Cu-dependent and programmed cell death pattern. However, the mechanism by which copper exposure might cause cell cuproptosis is largely unknown. We chose trophoblast cells as cell model and found that copper exposure causes trophoblast cell cuproptosis. In mechanism, copper exposure up-regulates lnc-HZ11 expression levels, which increases intracellular Cu2+ levels and causes trophoblast cell cuproptosis. Knockdown of lnc-HZ11 efficiently reduces intracellular Cu2+ levels and alleviate trophoblast cell cuproptosis, which could be further alleviated by co-treatment with DC or TEPA. These results discover novel toxicological effects of copper exposure and also provide potential target for protection trophoblast cells from cuproptosis in the presence of excessive copper exposure. • Cu exposure leads to trophoblast cell cuproptosis. • Cu exposure suppresses Fe-S cluster, lipoylation, and mitochondrial respiration. • Cu exposure up-regulates lnc-HZ11 expression levels. • Lnc-HZ11 induces cuproptosis in copper-exposed trophoblast cells. • Knockdown of lnc-HZ11 alleviates cuproptosis in copper-exposed trophoblast cells. [ABSTRACT FROM AUTHOR]