1. Observations on the Quantitative Determination of Indoleacetic Acid.
- Author
-
Gordon, S. A. and Paleg, L. G.
- Subjects
INDOLEACETIC acid ,PLANT extracts ,ACETIC acid ,ORGANIC compounds ,ORGANIC solvents ,SOLUTION (Chemistry) ,PLANT growth promoting substances ,CHROMATOGRAPHIC analysis - Abstract
The Fe-HClO
4 reaction for the assay of IAA has been extended to organic solvents, resulting in increased sensitivity and utility of the reaction. Direct colorimetric determinations of IAA in plant preparations are demonstrated to he inaccurate. However, by coupling the colorimetric assay with chromatography and elution. it is shown that IAA may be resolved from mixtures and quantitatively estimated. The method gives a less equivocal measure of IAA in plant extracts and enzymatic preparations than does direct colorimetry. [ABSTRACT FROM AUTHOR]- Published
- 1957
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