7,968 results on '"XYLOSE"'
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2. Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes.
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Carbonaro, Miriam, Aulitto, Martina, Mazurkewich, Scott, Fraia, Alessia Di, Contursi, Patrizia, Limauro, Danila, Larsbrink, Johan, and Fiorentino, Gabriella
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GEOBACILLUS stearothermophilus , *ENZYME stability , *HEMICELLULOSE , *MICROBIAL enzymes , *GLYCOSIDASES , *LIGNOCELLULOSE , *XYLOSE , *ETHANOL as fuel - Abstract
The valorization of lignocellulosic biomass, derived from various bio-waste materials, has received considerable attention as a sustainable approach to improve production chains while reducing environmental impact. Microbial enzymes have emerged as key players in the degradation of polysaccharides, offering versatile applications in biotechnology and industry. Among these enzymes, glycoside hydrolases (GHs) play a central role. Xylanases, in particular, are used in a wide range of applications and are essential for the production of xylose, which can be fermented into bioethanol or find use in many other industries. Currently, fungal secretomes dominate as the main reservoir of lignocellulolytic enzymes, but thermophilic microorganisms offer notable advantages in terms of enzyme stability and production efficiency. Here we present the genomic characterization of Geobacillus stearothermophilus GF16 to identify genes encoding putative enzymes involved in lignocellulose degradation. Thermostable GHs secreted by G. stearothermophilus GF16 were investigated and found to be active on different natural polysaccharides and synthetic substrates, revealing an array of inducible GH activities. In particular, the concentrated secretome possesses significant thermostable xylanase and β-xylosidase activities (5 ×103 U/L and 1.7 ×105 U/L, respectively), highlighting its potential for application in biomass valorization. We assessed the hemicellulose hydrolysis capabilities of various agri-food wastes using the concentrated secretome of the strain cultivated on xylan. An impressive 300-fold increase in xylose release compared to a commercially available cocktail was obtained with the secretome, underscoring the remarkable efficacy of this approach. [Display omitted] • Genome characterization of G. stearothermophilus GF16 reveals genes potentially involved in hemicellulose degradation. • The secretome of GF16 grown on a minimal medium enriched with 0.1 % xylan shows inducible xylanolytic properties. • G. stearothermophilus GF16 CAZymes are pivotal in converting agri-food wastes into value-added products, such as xylose. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Saccharification and structural changes in Areca catechu husk fiber.
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Vardhan, Harsh, Sasmal, Soumya, and Mohanty, Kaustubha
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BETEL palm , *XYLANASES , *FOURIER transform infrared spectroscopy , *FIELD emission electron microscopy , *AMORPHOUS substances , *BETEL nut , *XYLANS , *FEEDSTOCK - Abstract
Areca nut husk (ANH) holds promise as a viable biomass source for xylose production. Xylose is a precursor for various biochemicals. However, the recalcitrant nature of ANH makes saccharification more complex. To address this, lime and acid pretreatments were carried out to enhance the susceptibility of biomass to saccharification. Before this, a compositional analysis was conducted to determine the initial constituents of the feedstock. Saccharification was conducted under the following conditions: 2% (wV−1) substrate loading, 100 rpm agitation, and 30 °C hydrolysis temperature for 12 h hydrolysis time at pH 4.5 to 5.0. However, parameters like xylanase enzyme loading were varied to enhance the saccharification of the ANH. The results demonstrated that acid‐treated husk (ATH), lime‐treated husk (LTH), and raw husk (RH) achieved the highest yield (gg−1) of reducing sugar, approximately 90, 83, and 15%, respectively, at an enzyme loading of 15.0 IUg−1. Various analytical techniques, including Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), zeta potential, thermogravimetric analysis (TGA), X‐ray diffraction (XRD), and field emission scanning electron microscopy (FESEM) were used to examine structural changes in the native, pretreated, and saccharified residues of ANH. The analysis revealed that a significant amount of partial crystalline and amorphous cellulose in the ANH biomass was hydrolyzed during the saccharification process. However, saccharification also led to the removal of amorphous substances, disruption of the crystalline structure, and conversion of crystalline regions into amorphous domains. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Production of a bacterial secretome highly efficient for the deconstruction of xylans.
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Topalian, Juliana, Navas, Laura, Ontañon, Ornella, Valacco, Maria Pia, Noseda, Diego Gabriel, Blasco, Martín, Peña, Maria Jesus, Urbanowicz, Breeanna R., and Campos, Eleonora
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SUGARCANE , *PLANT cell walls , *WHEAT bran , *LIGNOCELLULOSE , *POLYSACCHARIDES , *XYLANS , *XYLANASES , *XYLOSE - Abstract
Bacteria within the Paenibacillus genus are known to secrete a diverse array of enzymes capable of breaking down plant cell wall polysaccharides. We studied the extracellular xylanolytic activity of Paenibacillus xylanivorans and examined the complete range of secreted proteins when grown on carbohydrate-based carbon sources of increasing complexity, including wheat bran, sugar cane straw, beechwood xylan and sucrose, as control. Our data showed that the relative abundances of secreted proteins varied depending on the carbon source used. Extracellular enzymatic extracts from wheat bran (WB) or sugar cane straw (SCR) cultures had the highest xylanolytic activity, coincidently with the largest representation of carbohydrate active enzymes (CAZymes). Scaling-up to a benchtop bioreactor using WB resulted in a significant enhancement in productivity and in the overall volumetric extracellular xylanase activity, that was further concentrated by freeze-drying. The enzymatic extract was efficient in the deconstruction of xylans from different sources as well as sugar cane straw pretreated by alkali extrusion (SCRe), resulting in xylobiose and xylose, as primary products. The overall yield of xylose released from SCRe was improved by supplementing the enzymatic extract with a recombinant GH43 β-xylosidase (EcXyl43) and a GH62 α-l-arabinofuranosidase (CsAbf62A), two activities that were under-represented. Overall, we showed that the extracellular enzymatic extract from P. xylanivorans, supplemented with specific enzymatic activities, is an effective approach for targeting xylan within lignocellulosic biomass. [ABSTRACT FROM AUTHOR]
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- 2024
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5. OPTIMIZATION OF CRYSTALLINE XYLOSE PRODUCTION FROM COFFEE CHERRY PROCESSING WASTE.
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Nur Yudiastuti, Silvia Oktavia, Handayani, Wiwik, Novita Sari, Elok Kurnia, Wijaya, Rizza, Brilliantina, Aulia, and Hasanuddin Slamet, Ahmad Haris
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FUNCTIONAL foods ,PRODUCTION engineering ,RESPONSE surfaces (Statistics) ,XYLANASES ,ACADEMIA ,COFFEE processing ,PLANT growing media ,SUBSTRATES (Materials science) ,XYLOSE - Abstract
Copyright of Environmental & Social Management Journal / Revista de Gestão Social e Ambiental is the property of Environmental & Social Management Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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6. Insights into the transglucosylation activity of α-glucosidase from Schwanniomyces occidentalis.
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Merdzo, Zoran, Narmontaite, Egle, Gonzalez-Alfonso, Jose L., Poveda, Ana, Jimenez-Barbero, Jesus, Plou, Francisco J., and Fernández-Lobato, María
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PHENOLS , *XYLOSE , *SUGARS , *YEAST , *RESVERATROL - Abstract
The α-glucosidase from Schwanniomyces occidentalis (GAM1p) was expressed in Komagataella phaffii to about 70 mg/L, and its transferase activity studied in detail. Several isomaltooligosaccharides (IMOS) were formed using 200 g/L maltose. The major production of IMOS (81.3 g/L) was obtained when 98% maltose was hydrolysed, of which 34.8 g/L corresponded to isomaltose, 26.9 g/L to isomaltotriose, and 19.6 g/L to panose. The addition of glucose shifted the IMOS synthesis towards products containing exclusively α(1 → 6)-linkages, increasing the production of isomaltose and isomaltotriose about 2–4 fold, enabling the formation of isomaltotetraose, and inhibiting that of panose to about 12 times. In addition, the potential of this enzyme to glycosylate 12 possible hydroxylated acceptors, including eight sugars and four phenolic compounds, was evaluated. Among them, only sucrose, xylose, and piceid (a monoglucosylated derivative of resveratrol) were glucosylated, and the main synthesised products were purified and characterised by MS and NMR. Theanderose, α(1 → 4)-D-glucosyl-xylose, and a mixture of piceid mono- and diglucoside were obtained with sucrose, xylose, and piceid as acceptors, respectively. Maximum production of theanderose reached 81.7 g/L and that of the glucosyl-xylose 26.5 g/L, whereas 3.4 g/L and only 1 g/L were produced of the piceid mono- and diglucoside respectively. Key points: • Overexpression of a yeast α-glucosidase producing novel molecules. • Yeast enzyme producing the heterooligosaccharides theanderose and glucosyl-xylose. • Glycosylation of the polyphenol piceid by a yeast α-glucosidase. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Development of a Two-Stage Bioprocess for the Production of Bioethanol from the Acid Hydrolysate of Brewer's Spent Grain.
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Vičević, Renata, Božinović, Marko, Zekić, Nikolina, Novak, Mario, Grgić, Dajana Kučić, Šalić, Anita, and Zelić, Bruno
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BREWER'S spent grain , *PENTOSE phosphate pathway , *RENEWABLE energy sources , *KLUYVEROMYCES marxianus , *SUGARCANE , *XYLOSE - Abstract
Bioethanol, an alcohol produced by microbial fermentation, is traditionally produced from sugar-rich plants such as sugar cane, sugar beet and maize. However, there is growing interest in the use of lignocellulose, an abundant and inexpensive renewable energy source, as a potential substitute for the production of biofuels and biochemicals. Yeast Saccharomyces cerevisiae, which is commonly used for ethanol fermentation, cannot cope with lignocellulose due to a lack of lignocellulolytic enzymes and the inefficient functioning of the pentose phosphate pathway. The aim of this research was to isolate yeasts that can efficiently produce bioethanol and valuable byproducts from both glucose and xylose in a two-stage fermentation process using brewer's spent grains. This approach should maximize sugar utilization and improve the economic viability of bioethanol production while contributing to waste valorization and sustainability. Kluyveromyces marxianus and Candida krusei were identified and tested with different initial concentrations of glucose and xylose. The results showed that both yeasts produced bioethanol from glucose but were inefficient with xylose, yielding valuable compounds, such as 2,3-butanediol and glycerol instead. A two-stage fermentation was then carried out with weak acidic hydrolysate from brewer's spent grain. In the first stage, glucose was fermented by S. cerevisiae to produce bioethanol; in the second stage, xylose was fermented by K. marxianus and C. krusei to obtain other valuable products. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Enzymatic Production of Glycerol from Glycolaldehyde and Formaldehyde.
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Seo, Pil‐Won, Kim, Ji‐Won, and Kim, Jeong‐Sun
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ALCOHOL dehydrogenase , *SUSTAINABLE design , *ESCHERICHIA coli , *FORMALDEHYDE , *XYLOSE - Abstract
Glycerol is a simple and important polyol in both biological and industrial field. To produce glycerol in an eco‐friendly manner, we established a one‐pot cascade reaction by fructose‐6‐phosphate aldolase (FSA) and aldehyde reductase (ALR) from formaldehyde (FALD) and its dimerized form glycolaldehyde (GALD) via L‐glyceraldehyde. For this, we characterized two FSAs at various conditions. Among them, FSA from Gilliamella apicola (GaFSA) has shown the higher L‐glyceraldehyde production activity in the phosphate buffer than the well‐known FSA from Escherichia coli (EcFSA) by more than 2 times. We also characterized two ALRs (xylose reductase from Candida tenuis; CtXR, the annotated DL‐glyceraldehyde reductase from Hypocrea jecorina; HjGLD) that specifically reduce L‐glyceraldehyde into glycerol. Bioproduction of glycerol from FALD and GALD was performed using GaFSA and CtXR, which produced ~8.8 mM glycerol from 25 mM of FALD and GALD (conversion yield of ~35 %). This suggested that a simple process would help in designing a green production of glycerol from a wasteful one‐carbon chemicals. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Molecular Modification Enhances Xylose Uptake by the Sugar Transporter KM_SUT5 of Kluyveromyces marxianus.
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Luo, Xiuyuan, Tao, Xi, Ran, Guangyao, Deng, Yuanzhen, Wang, Huanyuan, Tan, Liyan, and Pang, Zongwen
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KLUYVEROMYCES marxianus , *CARRIER proteins , *MOLECULAR docking , *XYLOSE , *BIOCHEMICAL substrates - Abstract
This research cloned and expressed the sugar transporter gene KM_SUT5 from Kluyveromyces marxianus GX-UN120, which displayed remarkable sugar transportation capabilities, including pentose sugars. To investigate the impact of point mutations on xylose transport capacity, we selected four sites, predicted the suitable amino acid sites by molecular docking, and altered their codons to construct the corresponding mutants, Q74D, Y195K, S460H, and Q464F, respectively. Furthermore, we conducted site-directed truncation on six sites of KM_SUT5p. The molecular modification resulted in significant changes in mutant growth and the D-xylose transport rate. Specifically, the S460H mutant exhibited a higher growth rate and demonstrated excellent performance across 20 g L−1 xylose, achieving the highest xylose accumulation under xylose conditions (49.94 μmol h−1 gDCW-1, DCW mean dry cell weight). Notably, mutant delA554-, in which the transporter protein SUT5 is truncated at position delA554-, significantly increased growth rates in both D-xylose and D-glucose substrates. These findings offer valuable insights into potential modifications of other sugar transporters and contribute to a deeper understanding of the C-terminal function of sugar transporters. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Hydrogen Production from Sugarcane Bagasse Pentose Liquor Fermentation Using Different Food/Microorganism and Carbon/Nitrogen Ratios under Mesophilic and Thermophilic Conditions.
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Mattiello-Francisco, Luísa, Ferreira, Filipe Vasconcelos, Peixoto, Guilherme, Mockaitis, Gustavo, and Zaiat, Marcelo
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HYDROGEN production ,BAGASSE ,SUGARCANE ,CLEAN energy ,FERMENTATION ,LIQUORS ,BUTYRATES - Abstract
Hydrogen is a well-known clean energy carrier with a high energetic yield. Its versatility allows it to be produced in diverse ways, including biologically. Specifically, dark fermentation takes advantage of organic wastes, such as agro-industrial residues, to obtain hydrogen. One of these harmful wastes that is poorly discharged into streams is sugarcane bagasse pentose liquor (SBPL). The present study aimed to investigate hydrogen generation from SBPL fermentation in batch reactors by applying different food/microorganism (2–10 F/M) and carbon/nitrogen (10–200 C/N) ratios under mesophilic and thermophilic conditions. Biohydrogen was produced in all pentose liquor experiments along with other soluble microbial products (SMPs): volatile fatty acids (VFAs) (at least 1.38 g L
−1 and 1.84 g L−1 by the average of C/N and F/M conditions, respectively) and alcohols (at least 0.67 g L−1 and 0.325 g L−1 by the average of C/N and F/M conditions, respectively). Thermophilic pentose liquor reactors (t-PLRs) showed the highest H2 production (H2 maximum: 1.9 ± 0.06 L in 100 C/N) and hydrogen yield (HY) (1.9 ± 0.54 moles of H2 moles of substrate−1 in 2 F/M) when compared to mesophilic ones (m-PLRs). The main VFA produced was acetate (>0.85 g L−1 , considering the average of both nutritional conditions), especially through the butyrate pathway, which was the most common metabolic route of experimental essays. Considering the level of acid dilution used in the pretreatment of bagasse (H2 SO4 (1%), 1.1 atm, 120 °C, 60 min), it is unlikely that toxic compounds such as furan derivatives, phenol-like substances (neither was measured), and acetate (<1.0 g L−1 ) hinder the H2 production in the pentose liquor reactors (PLRs). Sugarcane bagasse pentose liquor fermentation may become a suitable gateway to convert a highly polluting waste into a renewable feedstock through valuable hydrogen production. [ABSTRACT FROM AUTHOR]- Published
- 2024
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11. Exploring hemp seed hull biomass for an integrated C-5 biorefinery: Xylose and activated carbon
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Sreesha Malayil, Luke Loughran, Frederik Mendoza Ulken, and Jagannadh Satyavolu
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Xylose ,C-5 biorefinery ,Methane sulphonic acid ,Delignification ,Activated carbon ,Dilute acid hydrolysis ,Biochemistry ,QD415-436 - Abstract
Large quantities of hemp hulls can be completely utilized for creation of value-added products (cost effective biofuels and biochemicals) through a biorefinery approach. A sustainable approach in making xylose, a low calorie sweetener and high surface area activated carbons (AC) for super capacitors, attracts interest. The AC when leveraged as a co-product from biorefinery process makes it more cost effective and, in this paper, we discuss the production of xylose and AC from hemp seed hull with methane sulphonic acid (MSA) hydrolysis. Xylose recovery with MSA hydrolysis was 25.15 g/L when compared to the traditional sulphuric acid (SA) hydrolysis of 19.96 g/L at the same acid loading of 1.8 %. The scanning electron microscope (SEM) images and Fourier transform infrared (FT-IR) spectra indicate partial delignification along with hemicellulose hydrolysis responsible for high xylose recovery. Post hydrolysis fibers were KOH activated and carbonized to make AC. The MSA hydrolyzed and KOH activated fiber produced pure, fluffier and finer particle AC with a drastic increase in surface area 1 452 m2/g when compared to SA hydrolyzed of 977 m2/g. These results indicate the potential of MSA in dilute acid hydrolysis of biomass for xylose recovery and production of high surface area activated carbon. From a production standpoint this can lead to increased use of sustainable low-cost agricultural biomass for making high surface area AC as components in supercapacitors.
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- 2024
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12. 'We're Just Getting Started'.
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Wheatley, Harlen
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BOURBON whiskey ,ENGLISH-speaking countries ,CARGO ships ,TECHNOLOGICAL innovations ,XYLOSE ,DISTILLERIES - Published
- 2024
13. Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway
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Yali Qiu, Wei Liu, Meiling Wu, Haodong Bao, Xinhua Sun, Qin Dou, Hongying Jia, Weifeng Liu, and Yu Shen
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Saccharomyces cerevisiae ,Xylose ,Ethanol ,NADPH ,Glyceraldehyde-3-phosphate dehydrogenase ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 - Abstract
Full conversion of glucose and xylose from lignocellulosic hydrolysates is required for obtaining a high ethanol yield. However, glucose and xylose share flux in the pentose phosphate pathway (PPP) and glycolysis pathway (EMP), with glucose having a competitive advantage in the shared metabolic pathways. In this work, we knocked down ZWF1 to preclude glucose from entering the PPP. This reduced the [NADPH] level and disturbed growth on both glucose or xylose, confirming that the oxidative PPP, which begins with Zwf1p and ultimately leads to CO2 production, is the primary source of NADPH in both glucose and xylose. Upon glucose depletion, gluconeogenesis is necessary to generate glucose-6-phosphate, the substrate of Zwf1p. We re-established the NADPH regeneration pathway by replacing the endogenous NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene TDH3 with heterogenous NADP + -GAPDH genes GDH, gapB, and GDP1. Among the resulting strains, the strain BZP1 (zwf1Δ, tdh3::GDP1) exhibited a similar xylose consumption rate before glucose depletion, but a 1.6-fold increased xylose consumption rate following glucose depletion compared to the original strain BSGX001, and the ethanol yield for total consumed sugars of BZP1 was 13.5% higher than BSGX001. This suggested that using the EMP instead of PPP to generate NADPH reduces the wasteful metabolic cycle and excess CO2 release from oxidative PPP. Furthermore, we used a copper-repressing promoter to modulate the expression of ZWF1 and optimize the timing of turning off the ZWF1, therefore, to determine the competitive equilibrium between glucose-xylose co-metabolism. This strategy allowed fast growth in the early stage of fermentation and low waste in the following stages of fermentation.
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- 2024
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14. Enhanced reducing sugar production by blending hydrolytic enzymes from Aspergillus fumigatus to improve sugarcane bagasse hydrolysis.
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Saroj, Paramjeet, P, Manasa, and Narasimhulu, Korrapati
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FOURIER transform infrared spectroscopy ,HYDROLASES ,RESPONSE surfaces (Statistics) ,ASPERGILLUS fumigatus ,BIOMASS production ,CELLULASE ,LIGNIN structure ,XYLANASES - Abstract
Biomass pretreatment for the production of second-generation (2G) ethanol and biochemical products is a challenging process. The present study investigated the synergistic efficiency of purified carboxymethyl cellulase (CMCase), β-glucosidase, and xylanase from Aspergillus fumigatus JCM 10253 in the hydrolysis of alkaline-pretreated sugarcane bagasse (SCB). The saccharification of pretreated SCB was optimised using a combination of CMCase and β-glucosidase (C + β; 1:1) and addition of xylanase (C + β + xyl; 1:1:1). Independent and dependent variables influencing enzymatic hydrolysis were investigated using response surface methodology (RSM). Hydrolysis using purified CMCase and β-glucosidase achieved yields of 18.72 mg/mL glucose and 6.98 mg/mL xylose. Incorporation of xylanase in saccharification increased the titres of glucose (22.83 mg/mL) and xylose (9.54 mg/mL). Furthermore, characterisation of SCB biomass by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy respectively confirmed efficient structural disintegration and revealed the degree of crystallinity and spectral characteristics. Therefore, depolymerisation of lignin to produce high-value chemicals is essential for sustainable and competitive biorefinery development. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Optimizing lactic acid production for agricultural wastes revalorization via sugars hydrothermal conversion with zirconia-based catalysts: response surface methodology.
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Piovano, Federico A., Aspromonte, Soledad G., Gross, Martin S., Bergamini, Carina, and Boix, Alicia V.
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The hydrothermal conversion of a complex sugars solution comprising glucose, arabinose, and xylose with zirconia-based catalysts to produce lactic acid (LA) was studied. Catalysts were synthesized by a simple template-assisted sol–gel method. Alternative template removal procedures, such as refluxing ethanol extraction, were compared to the conventional direct calcination. The catalyst obtained after ethanol extraction (Z-Ex) reported the highest specific surface area (323 m
2 g−1 ), total pore volume (0.21 cm3 g−1 ) and density of acid sites (1.111 µmol m−2 ), with a significant contribution of strong acid sites. On the other hand, template removal by thermal treatment transformed the hydrous zirconia into crystalline tetragonal phase, reducing the specific surface area and removing most of the acid sites. Catalytic tests were carried out in a stirred batch reactor, resulting Z-Ex the most selective towards LA production. A four-factor experimental design was performed for Z-Ex catalyst by varying temperature, reaction time, initial pressure, and catalyst/reactants ratio. Results were analyzed using the response surface methodology (RSM) to maximize the LA production. A molar yield of 61.2% towards LA and complete sugars conversion were reached at optimized operating conditions: 189 °C, 208 min, 0.5 g catalyst g−1 reactants, and 10 bar N2 . Additionally, fitting models for main by-products molar yields (furans, glyceraldehyde, glycolic, formic, acetic, and levulinic acids) were obtained with good accuracy prediction. All responses were jointly studied to understand the reaction mechanisms involved in generation of intermediaries and undesired degradation products. [ABSTRACT FROM AUTHOR]- Published
- 2024
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16. Deficiency of β-xylosidase activity in Aspergillus luchuensis mut. kawachii IFO 4308.
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Zhu, Enkang, Hiramatsu, Kentaro, Inoue, Taiga, Mori, Kazuki, Tashiro, Kosuke, Fujita, Kiyotaka, Karashima, Takefumi, Takashita, Hideharu, Okutsu, Kayu, Yoshizaki, Yumiko, Takamine, Kazunori, Tamaki, Hisanori, and Futagami, Taiki
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ASPERGILLUS , *XYLOSE , *GENETIC mutation , *BARLEY , *GENOMES - Abstract
In this study, we investigated a deleterious mutation in the β-xylosidase gene, xylA (AkxylA), in Aspergillus luchuensis mut. kawachii IFO 4308 by constructing an AkxylA disruptant and complementation strains of AkxylA and xylA derived from A. luchuensis RIB2604 (AlxylA), which does not harbor the mutation in xylA. Only the AlxylA complementation strain exhibited significantly higher growth and substantial β-xylosidase activity in medium containing xylan, accompanied by an increase in XylA expression. This resulted in lower xylobiose and higher xylose concentrations in the mash of barley shochu. These findings suggest that the mutation in xylA affects xylose levels during the fermentation process. Because the mutation in xylA was identified not only in the genome of strain IFO 4308 but also the genomes of other industrial strains of A. luchuensis and A. luchuensis mut. kawachii , these findings enhance our understanding of the genetic factors that affect the fermentation characteristics. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Engineering of Saccharomyces cerevisiae for enhanced metabolic robustness and L-lactic acid production from lignocellulosic biomass.
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Choi, Bohyun, Tafur Rangel, Albert, Kerkhoven, Eduard J., and Nygård, Yvonne
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LACTIC acid , *LIGNOCELLULOSE , *SACCHAROMYCES cerevisiae , *POLYLACTIC acid , *PYRUVATE kinase , *BIOMASS - Abstract
Metabolic engineering for high productivity and increased robustness is needed to enable sustainable biomanufacturing of lactic acid from lignocellulosic biomass. Lactic acid is an important commodity chemical used for instance as a monomer for production of polylactic acid, a biodegradable polymer. Here, rational and model-based optimization was used to engineer a diploid, xylose fermenting Saccharomyces cerevisiae strain to produce L-lactic acid. The metabolic flux was steered towards lactic acid through the introduction of multiple lactate dehydrogenase encoding genes while deleting ERF2 , GPD1 , and CYB2. A production of 93 g/L of lactic acid with a yield of 0.84 g/g was achieved using xylose as the carbon source. To increase xylose utilization and reduce acetic acid synthesis, PHO13 and ALD6 were also deleted from the strain. Finally, CDC19 encoding a pyruvate kinase was overexpressed, resulting in a yield of 0.75 g lactic acid/g sugars consumed, when the substrate used was a synthetic lignocellulosic hydrolysate medium, containing hexoses, pentoses and inhibitors such as acetate and furfural. Notably, modeling also provided leads for understanding the influence of oxygen in lactic acid production. High lactic acid production from xylose, at oxygen-limitation could be explained by a reduced flux through the oxidative phosphorylation pathway. On the contrast, higher oxygen levels were beneficial for lactic acid production with the synthetic hydrolysate medium, likely as higher ATP concentrations are needed for tolerating the inhibitors therein. The work highlights the potential of S. cerevisiae for industrial production of lactic acid from lignocellulosic biomass. [Display omitted] • A xylose fermenting S. cerevisiae strain was engineered to produce L-lactic acid. • Rational and model-guided design was applied to increase lactic acid production. • 93 g/L of lactic acid at a yield of 0.84 g/g was produced from xylose. • A yield of 0.75 g/g was achieved in a synthetic lignocellulosic hydrolysate. • Modeling was used to understand the influence of oxygen in lactic acid production. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Enzymatic production of xylose esters using degummed soybean oil fatty acids following a hydroesterification strategy.
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Miranda, Felipe Cardoso, Oliveira, Kaíque Souza Gonçalves Cordeiro, Tardioli, Paulo Waldir, Fernandez-Lafuente, Roberto, and Guimarães, José Renato
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LIPASES , *SOY oil , *FATTY acid esters , *XYLOSE , *ESTERS , *FATTY acids - Abstract
The enzymatic synthesis of xylose fatty acid esters was carried out in two steps: hydrolysis of degummed soybean oil (DSO) and esterification of the produced and purified free fatty acids (FFA) with xylose. Different lipases immobilized on a hydrophobic support were evaluated in the hydrolysis of DSO and an experimental design was used to optimize the reaction. Eversa® Transform 2.0 (EV-Purolite) yielded 93% conversion after 6 h using biocatalyst load of 6.53 wt%, water/oil mass ratio of 5.5 and temperature of 48.4 °C. High operational stability for EV-Purolite was found using a sequential batch strategy and the possibility of concentrating glycerol. In the esterification stage, lipase from Thermomyces lanuginosus (TLL-Purolite) and lipase B from Candida antarctica showed better performance than lipase from porcine pancreas and EV-Purolite. About 17% of FFAs (0.85 molecules of FAA per xylose molecule) was consumed using an enzyme load of 5 wt% of TLL-Purolite and FFA/xylose molar ratio of 5. Sequential esterification batches showed low operational stability of TTL-Purolite due to the desorption of TLL from the support. The final mixture of the reaction medium containing xylose fatty acid esters showed emulsifying properties similar to those of commercial surfactant. [Display omitted] • Xylose fatty acid esters production by degummed soybean oil hydroesterification. • Hydrolysis catalyzed by immobilized Eversa produced 92% of FFAs after 6 h. • Reuse of immobilized Eversa for five consecutive 3 h-batches of hydrolysis. • Modification of 85% xylose as monoester using immobilized TLL. • Xylose esters showed emulsifying properties similar to commercial surfactants. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9.
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Wang, Siqi, Liu, Yujie, Guo, Hongfu, Meng, Yan, Xiong, Weini, Liu, Ruihua, and Yang, Chao
- Abstract
Microbial production of polyhydroxyalkanoate (PHA) is greatly restricted by high production cost arising from high‐temperature sterilization and expensive carbon sources. In this study, a low‐cost PHA production platform was established from Halomonas cupida J9. First, a marker‐less genome‐editing system was developed in H. cupida J9. Subsequently, H. cupida J9 was engineered to efficiently utilize xylose for PHA biosynthesis by introducing a new xylose metabolism module and blocking xylonate production. The engineered strain J9UΔxylD‐P8xylA has the highest PHA yield (2.81 g/L) obtained by Halomonas with xylose as the sole carbon source so far. This is the first report on the production of short‐ and medium‐chain‐length (SCL‐co‐MCL) PHA from xylose by Halomonas. Interestingly, J9UΔxylD‐P8xylA was capable of efficiently utilizing glucose and xylose as co‐carbon sources for PHA production. Furthermore, fed‐batch fermentation of J9UΔxylD‐P8xylA coupled to a glucose/xylose co‐feeding strategy reached up to 12.57 g/L PHA in a 5‐L bioreactor under open and unsterile condition. Utilization of corn straw hydrolysate as the carbon source by J9UΔxylD‐P8xylA reached 7.0 g/L cell dry weight (CDW) and 2.45 g/L PHA in an open fermentation. In summary, unsterile production in combination with inexpensive feedstock highlights the potential of the engineered strain for the low‐cost production of PHA from lignocellulose‐rich agriculture waste. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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20. Spathaspora marinasilvae sp. nov., a xylose‐fermenting yeast isolated from galleries of passalid beetles and rotting wood in the Amazonian rainforest biome.
- Author
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Barros, Katharina O., Batista, Thiago M., Soares, Rafaela C. C., Lopes, Mariana R., Alvarenga, Flávia B. M., Souza, Gisele F. L., Abegg, Maxwel A., Santos, Ana Raquel O., Góes‐Neto, Aristóteles, Hilário, Heron O., Moreira, Rennan G., Franco, Glória R., Lachance, Marc‐André, and Rosa, Carlos A.
- Abstract
Four yeast isolates were obtained from rotting wood and galleries of passalid beetles collected in different sites of the Brazilian Amazonian Rainforest in Brazil. This yeast produces unconjugated allantoid asci each with a single elongated ascospore with curved ends. Sequence analysis of the internal transcribed spacer‐5.8 S region and the D1/D2 domains of the large subunit ribosomal RNA (rRNA) gene showed that the isolates represent a novel species of the genus Spathaspora. The novel species is phylogenetically related to a subclade containing Spathaspora arborariae and Spathaspora suhii. Phylogenomic analysis based on 1884 single‐copy orthologs for a set of Spathaspora species whose whole genome sequences are available confirmed that the novel species represented by strain UFMG‐CM‐Y285 is phylogenetically close to Sp. arborariae. The name Spathaspora marinasilvae sp. nov. is proposed to accommodate the novel species. The holotype of Sp. marinasilvae is CBS 13467 T (MycoBank 852799). The novel species was able to accumulate xylitol and produce ethanol from d‐xylose, a trait of biotechnological interest common to several species of the genus Spathaspora. Take‐away: The novel species Spathaspora marinasilvae was isolated, identified, and formally described.The genome sequence of Spathaspora marinasilvae is reported.Spathaspora marinasilvae assimilated and fermented d‐xylose with ethanol production and accumulation of xylitol. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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21. Use of xylosidase 3C from Segatella baroniae to discriminate xylan non-reducing terminus substitution characteristics.
- Author
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St John, Franz J., Bynum, Loreen, Tauscheck, Dante A., and Crooks, Casey
- Subjects
- *
FUNGAL enzymes , *BACTERIAL enzymes , *GLUCURONIC acid , *XYLANS , *XYLOSE , *PREVOTELLA - Abstract
Objective: New characterized carbohydrate-active enzymes are needed for use as tools to discriminate complex carbohydrate structural features. Fungal glycoside hydrolase family 3 (GH3) β-xylosidases have been shown to be useful for the structural elucidation of glucuronic acid (GlcA) and arabinofuranose (Araf) substituted oligoxylosides. A homolog of these GH3 fungal enzymes from the bacterium Segatella baroniae (basonym Prevotella bryantii), Xyl3C, has been previously characterized, but those studies did not address important functional specificity features. In an interest to utilize this enzyme for laboratory methods intended to discriminate the structure of the non-reducing terminus of substituted xylooligosaccharides, we have further characterized this GH3 xylosidase. Results: In addition to verification of basic functional characteristics of this xylosidase we have determined its mode of action as it relates to non-reducing end xylose release from GlcA and Araf substituted oligoxylosides. Xyl3C cleaves xylose from the non-reducing terminus of β-1,4-xylan until occurrence of a penultimate substituted xylose. If this substitution is O2 linked, then Xyl3C removes the non-reducing xylose to leave the substituted xylose as the new non-reducing terminus. However, if the substitution is O3 linked, Xyl3C does not hydrolyze, thus leaving the substitution one-xylose (penultimate) from the non-reducing terminus. Hence, Xyl3C enables discrimination between O2 and O3 linked substitutions on the xylose penultimate to the non-reducing end. These findings are contrasted using a homologous enzyme also from S. baroniae, Xyl3B, which is found to yield a penultimate substituted nonreducing terminus regardless of which GlcA or Araf substitution exists. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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22. Construction and characterization of a promoter library with varying strengths to enhance acetoin production from xylose in Serratia marcescens.
- Author
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Liu, Jie, Liu, Di, Sun, Tingting, Fan, Tai‐Ping, and Cai, Yujie
- Subjects
- *
XYLOSE , *SERRATIA marcescens , *ACETOIN , *CARRIER proteins , *METABOLITES , *ENTEROBACTERIACEAE , *MONOCARBOXYLATE transporters - Abstract
Serratia marcescens is utilized as a significant enterobacteria in the production of various high‐value secondary metabolites. Acetoin serves as a crucial foundational compound of development and finds application in a broad range of fields. Furthermore, S. marcescens HBQA‐7 is capable of utilizing xylose as its exclusive carbon source for acetoin production. The objective of this study was to utilize a constitutive promoter screening strategy to enhance both xylose utilization and acetoin production in S. marcescens HBQA‐7. By utilizing RNA‐seq, we identified the endogenous constitutive promoter P6 that is the most robust, which facilitated the overexpression of the sugar transporter protein GlfL445I, α‐acetyl lactate synthase, and α‐acetyl lactate decarboxylase, respectively. The resultant recombinant strains exhibited enhanced xylose utilization rates and acetoin yields. Subsequently, a recombinant plasmid, denoted as pBBR1MCS‐P6‐glfL445IalsSalsD, was constructed, simultaneously expressing the aforementioned three genes. The resulting recombinant strain, designated as S3, demonstrated a 1.89‐fold boost in xylose consumption rate compared with the original strain during shake flask fermentation. resulting in the accumulation of 7.14 g/L acetoin in the final fermentation medium. Subsequently, in a 5 L fermenter setup, the acetoin yield reached 48.75 g/L, corresponding to a xylose‐to‐acetoin conversion yield of 0.375 g/g. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
23. Composition Analysis and Environmental Factors Influencing Biomass Quality: a Comparative Study of Montana-Grown Biomasses.
- Author
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Scheffel, Aidan J., Johnsrude, Lauren M., Allen, Brett L., and Wettstein, Stephanie G.
- Subjects
- *
SUSTAINABILITY , *FACTOR analysis , *BIOMASS , *CORN stover , *ARABINOSE , *ENERGY crops , *CHEMICAL yield , *SWITCHGRASS - Abstract
In order to obtain high yields of chemicals and fuels from biomass, feedstocks need to be selected that contain high amounts of glucose, xylose, or lignin, depending on the end product. The first objective of this study was to evaluate the biomass composition of 20 samples from 11 broadleaf and grass crop species grown at two distinct sites in Montana. Another objective was to investigate the influence of growing location and seed type to determine which cultivars may be best for renewable chemical production in the northern Great Plains. There was a significant effect due to the growing location as the biomass samples from the site that had higher precipitation exhibited a significantly higher acid-insoluble lignin (23.8%) content compared to the biomass samples from the lower precipitation site (20.0%). Additionally, the 357 napus had a significantly higher amount of glucose (1.8% more) and acid-soluble lignin (0.2% more) than compared to the 940 napus both grown at the Froid site that may be attributed to genetic modifications. In terms of sugar content for potential upgrading, the grasses had significantly more glucose (36.1% total), xylose (24.3%), and arabinose (2.3%) than the Brassicaceae biomasses, but the lignin content was not significantly different. Switchgrass, in particular, had the highest total sugar content of close to 70 wt%. Understanding the impact of growing location on biomass composition is an important consideration for optimizing biomass utilization strategies and developing sustainable bioenergy production systems. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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24. Effects of Zn2+ and Mn2+ on the photo-fermentative performance of HY01 in biohydrogen production from xylose fermentation.
- Author
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Zhou, Yuheng, Deng, Hui, Wang, Xiaohui, Huang, Xubo, and Hu, Yuntao
- Subjects
XYLOSE ,FERMENTATION ,HYDROGEN production ,PHOTOSYNTHETIC bacteria ,ENERGY transfer ,ZINC ions - Abstract
The addition of specific ions has a significant effect on the characteristics and kinetics of hydrogen production by photofermentation (PFHP). In this article, a new basic research investigated by us find that adjusting the addition ratio of Zn
2+ and Mn2+ ions in the R. sphaeroides HY01 system can effectively improve their biohydrogen production from xylose. The largest biohydrogen yield can be obtained as high as 219.2 mL/g xylose at concentration levels of 12 mg/L Zn2+ and 8 mg/L Mn2+ . The shortest lag time is 5.76 h, along with most of the organic energy in the substrate transferred to H2 . To evaluate the effect of Zn2+ and Mn2+ , the Gompertz model with appropriately modification is applied on the photosynthetic bacteria R. sphaeroides HY01, and the corresponding constants are obtained by fitting the model, which is of great guiding significance for the optimization of fermentation conditions. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
25. Untargeted metabolomics coupled with genomics in the study of sucrose and xylose metabolism in Pectobacterium betavasculorum.
- Author
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Smoktunowicz, Magdalena, Wawrzyniak, Renata, Jonca, Joanna, Waleron, Małgorzata, and Waleron, Krzysztof
- Subjects
XYLOSE ,SUCROSE ,ERWINIA ,AMINO acid metabolism ,METABOLOMICS ,PLANT cell walls ,GENOMICS - Abstract
Introduction: Pectobacterium betavasculorum is a member of the Pectobacerium genus that inhabits a variety of niches and is found in all climates. Bacteria from the Pectobacterium genus can cause soft rot disease on various plants due to the secretion of plant cell wall degrading enzymes (PCWDEs). The species P. betavasculorum is responsible for the vascular necrosis of sugar beet and soft rot of many vegetables. It also infects sunflowers and artichokes. The main sugar present in sugar beet is sucrose while xylose is one of the main sugars in artichoke and sunflower. Methods: In our work, we applied metabolomic studies coupled with genomics to investigate the metabolism of P. betavasculorum in the presence of xylose and sucrose as the only carbon source. The ability of the strains to use various sugars as the only carbon source were confirmed by the polypyridyl complex of Ru(II) method in 96-well plates. Results: Our studies provided information on the metabolic pathways active during the degradation of those substrates. It was observed that different metabolic pathways are upregulated in the presence of xylose in comparison to sucrose. Discussion: The presence of xylose enhances extracellular metabolism of sugars and glycerol as well as stimulates EPS and IPS synthesis. In contrast, in the presence of sucrose the intensive extracellular metabolism of amines and amino acids is promoted. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
26. Enhancement mechanism of glycation with l‐arabinose and xylose on texture properties of silver carp mince gel.
- Author
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Liu, Junya, Zhu, Xueshen, and Shi, Wenzheng
- Subjects
- *
SILVER carp , *XYLOSE , *IMMOBILIZED proteins , *NUCLEAR magnetic resonance , *PROTEIN structure , *WATER distribution - Abstract
BACKGROUND: Glycation is a green processing technology. Based on our previous studies, glycation with l‐arabinose and xylose was beneficial to enhance the texture properties of silver carp mince (SCM) gels. However, the possible enhancement mechanism remained unclear. Therefore, in this study, SCM gels with different types of reducing sugar (glucose, l‐arabinose, and xylose) were prepared based on our previous study. The possible mechanism of texture enhancement of SCM gels was analyzed by investigating the changes in water distribution, protein structures, and microstructure in the gel system. RESULTS: The glycation of l‐arabinose and xylose enhanced the hardness, cohesiveness, chewiness, and resilience of SCM gels. Hardness increased from 1883.04 (control group) to 3624.54 (l‐arabinose group) and 4348.18 (xylose group). Low‐field nuclear magnetic resonance (LF‐NMR) showed that glycation promoted the tight binding of immobilized water to proteins. Raman spectroscopic analysis showed that glycation increased the surface hydrophobicity and promoted the formation of disulfide bonds. Scanning electron microscopy (SEM) showed that glycation promoted the formation of uniform and dense three‐dimensional network structure in SCM gels. CONCLUSION: In summary, glycation enhanced the binding ability of immobilized water to proteins, improved the surface hydrophobicity, promoted the formation of disulfide bonds, and led to a more uniform and dense gel network structure of proteins, thus enhancing the texture properties of SCM gels. This research provided a theoretical basis for a better understanding of the mechanism of the effect of glycation on the quality of gel products and also provided technical support for the application of l‐arabinose and xylose in new functional gel foods. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
27. Fermentation of Sugar by Thermotolerant Hansenula polymorpha Yeast for Ethanol Production.
- Author
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Karim, Adnan Asad, Martínez-Cartas, Mª Lourdes, and Cuevas-Aranda, Manuel
- Subjects
ETHANOL ,FERMENTATION ,YEAST ,FRUCTOSE ,SUGAR ,RECOMBINANT proteins - Abstract
Hansenula polymorpha is a non-conventional and thermo-tolerant yeast that is well-known for its use in the industrial production of recombinant proteins. However, research to evaluate this yeast's potential for the high-temperature fermentation of sugar to produce alcohols for biofuel applications is limited. The present work investigated a wild-type H. polymorpha strain (DSM 70277) for the production of ethanol at a temperature of 40 °C under limited oxygen presence by using a batch fermentation reactor. Fermentation experiments were performed using three types of sugar (glucose, fructose, xylose) as substrates with two initial inoculum concentrations (1.1 g·L
−1 and 5.0 g·L−1 ). The maximum specific growth rates of H. polymorpha yeast were 0.121–0.159 h−1 for fructose, 0.140–0.175 h−1 for glucose, and 0.003–0.009 h−1 for xylose. The biomass volumetric productivity was 0.270–0.473 g·L−1 h−1 (fructose), 0.185–0.483 g·L−1 h−1 (glucose), and 0.001–0.069 g·L−1 h−1 (xylose). The overall yield of ethanol from glucose (0.470 g·g−1 ) was higher than that from fructose (0.434 g·g−1 ) and xylose (0.071 g·g−1 ). The H. polymorpha yeast exhibited different behavior and efficacy regarding the use of glucose, fructose, and xylose as substrates for producing ethanol. The present knowledge could be applied to improve the fermentation process for valorization of waste biomass to produce bioethanol. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
28. Glucose and Xylose Utilization by Bacillus Cereus AS1 During Anaerobic Fermentation.
- Author
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Sibiya, Nhlamulo G., Chirwa, Evans M. N., Daramola, Michael, and Brink, Hendrik G.
- Subjects
BACILLUS cereus ,FERMENTATION ,ANAEROBIC digestion ,XYLOSE ,GLUCOSE - Abstract
The aim was to determine the growth potential of Bacillus cereus using glucose and xylose as the substrates. In this study a bacterial isolate was identified by 16s rRNA gene sequencing. Bacillus.cereus can metabolise glucose and xylose as the sole carbon sources during anaerobic fermentation. The biomass growth profile showed a lag of about 8 h, followed by an increase in the biomass. The growth profile was better on glucose than xylose. The maximum biomass concentration achieved was 3.64 g/L and 2.62 g/L using glucose and xylose, respectively. The higher the initial substrate concentration the higher the growth of the bacteria. The growth of B. cereus was accompanied by a decrease in the ORP. Its ability to metabolise xylose proves that it has the potential to convert biomass-derived feed for possible applications as a bio-fertilizer. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
29. Characterization of Grape Pomace Extract Microcapsules: The Influence of Carbohydrate Co-Coating on the Stabilization of Goat Whey Protein as a Primary Coating.
- Author
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Perković, Gabriela, Martinović, Josipa, Šelo, Gordana, Bucić-Kojić, Ana, Planinić, Mirela, and Ambrus, Rita
- Subjects
WHEY proteins ,MALTODEXTRIN ,GRAPES ,CARBOHYDRATES ,GOAT milk ,SPRAY drying ,PHENOLS ,SURFACE coatings - Abstract
Both grape pomace and whey are waste products from the food industry that are rich in valuable ingredients. The utilization of these two by-products is becoming increasingly possible as consumer awareness of upcycling increases. The biological activities of grape pomace extract (GPE) are diverse and depend on its bioavailability, which is influenced by processes in the digestive system. In this work, goat whey protein (GW) was used as the primary coating to protect the phenolic compounds of GPE during the spray drying process. In addition, trehalose (T), sucrose (S), xylose (X), and maltodextrin (MD) were added to the goat whey proteins as co-coatings and protein stabilizers. All spray drying experiments resulted in microcapsules (MC) with a high encapsulation efficiency (77.6–95.5%) and yield (91.5–99.0%) and almost 100% recovery of phenolic compounds during the release test. For o-coumaric acid, the GW-coated microcapsules (MC) showed a bioavailability index of up to 731.23%. A semi-crystalline structure and hydrophilicity were characteristics of the MC coated with 10% T, S, X, or 5% MD. GW alone or in combination with T, S, MD, or X proved to be a promising carrier for polyphenols from grape pomace extract and ensured good bioavailability of these natural antioxidants. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
30. Selective production of methylindan and tetralin with xylose or hemicellulose.
- Author
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Zou, Zhufan, Yu, Zhenjie, Guan, Weixiang, Liu, Yanfang, Yao, Yumin, Han, Yang, Li, Guangyi, Wang, Aiqin, Cong, Yu, Liang, Xinmiao, Zhang, Tao, and Li, Ning
- Subjects
HEMICELLULOSE ,XYLOSE ,JET fuel ,BIMETALLIC catalysts ,AGRICULTURAL wastes ,FUEL additives - Abstract
Indan and tetralin are widely used as fuel additives and the intermediates in the manufacture of thermal-stable jet fuel, many chemicals, medicines, and shockproof agents for rubber industry. Herein, we disclose a two-step route to selectively produce 5-methyl-2,3-dihydro-1H-indene (abbreviated as methylindan) and tetralin with xylose or the hemicelluloses from agricultural or forestry waste. Firstly, cyclopentanone (CPO) was selectively formed with ~60% carbon yield by the direct hydrogenolysis of xylose or hemicelluloses on a non-noble bimetallic Cu-La/SBA-15 catalyst. Subsequently, methylindan and tetralin were selectively produced with CPO via a cascade self-aldol condensation/rearrangement/aromatization reaction catalyzed by a commercial H-ZSM-5 zeolite. When we used cyclohexanone (another lignocellulosic cycloketone) in the second step, the main product switched to dimethyltetralin. This work gives insights into the selective production of bicyclic aromatics with lignocellulose. Indan and tetralin are used as fuel additives and intermediates in the manufacture of many products, including thermal-stable jet fuel. Here the authors report a route for the synthesis of methylindan and tetralin by the hydrogenolysis of xylose or hemicellulose over a non-noble metal catalyst, followed by further reaction over zeolite H-ZSM-5. Reviewer recognition: [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
31. Structural Analysis of Xylose Isomerase from Streptomyces avermitilis.
- Author
-
Nam, Ki Hyun
- Subjects
HIGH-fructose corn syrup ,STREPTOMYCES ,HEMICELLULOSE ,XYLOSE ,ALDOSES ,PROTEIN engineering ,ISOMERASES - Abstract
Xylose isomerase (XI, also known as glucose isomerase) is an oxidoreductase that interconverts aldoses and ketoses. XI catalyzes the reversible isomerization of D-glucose and D-xylose into D-fructose and D-xylulose, respectively. The molecular function of XI is widely applied in producing high-fructose corn syrup (HFCS) in the food industry and bioethanol from hemicellulose in the biofuel industry. The structural information of XI from diverse strains is important for understanding molecular properties that can provide insights into protein engineering to improve enzyme efficiency. To extend the knowledge of the structural information on XI, the crystal structure of XI from Streptomyces avermitilis (SavXI) was determined at a 2.81 Å resolution. SavXI containing TIM barrel and extended α-helix domains formed the tetrameric assembly. The two metal-binding sites and their coordinating residues showed diverse conformations, providing the structural flexibility of the active site of SavXI. The structural comparison of SavXI and XI homologs exhibited unique metal-binding sites and conformations of the C-terminal α-helix domain. These structural results extend our knowledge of the molecular flexibility and mechanism of the XI family. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
32. Recognition of a Single β-D-Xylopyranose Molecule by Xylanase GH11 from Thermoanaerobacterium saccharolyticum.
- Author
-
Nam, Ki Hyun
- Subjects
SINGLE molecules ,XYLANS ,XYLANASES ,HYDROGEN bonding interactions ,MOLECULAR recognition ,HYDROXYL group - Abstract
The endo-β-1,4-xylanase glycosyl hydrolase (GH11) decomposes the backbone of xylan into xylooligosaccharides or xylose. These enzymes are important for industrial applications in the production of biofuel, feed, food, and value-added materials. β-D-xylopyranose (XYP, also known as β-D-xylose) is the fundamental unit of the substrate xylan, and understanding its recognition is fundamental for the initial steps of GH11's molecular mechanism. However, little is known about the recognition of a single XYP molecule by GH11. In this study, the crystal structures of GH11 from Thermoanaerobacterium saccharolyticum (TsaGH11) complexed with an XYP molecule were determined at a resolution of 1.7–1.9 Å. The XYP molecule binds to subsite −2 of the substrate-binding cleft. The XYP molecule is mainly stabilized by a π–π interaction with the conserved Trp36 residue. The O2 and O3 atoms of XYP are stabilized by hydrogen bond interactions with the hydroxyl groups of Tyr96 and Tyr192. The conformation of the thumb domain of TsaGH11 does not play a critical role in XYP binding, and XYP binding induces a shift in the thumb domain of TsaGH11 toward the XYP molecule. A structural comparison of TsaGH11 with other GH11 xylanases revealed that the XYP molecule forms π–π stacking with the center between the phenyl and indoline ring of Trp36, whereas the XYP molecule unit from xylobiose or xylotetraose forms π–π stacking with the indoline of Trp36, which indicates that the binding modes of the substrate and XYP differ. These structural results provide a greater understanding of the recognition of XYP by the GH11 family. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
33. Short-term Meyerozyma caribbica Y67 adaptation in sugarcane trash hemicellulosic hydrolysate for xylitol production.
- Author
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Pramasari, Dwi Ajias, Oktaviani, Maulida, Fahrezi, M. Zuvan Maulana, Thontowi, Ahmad, Kanti, Atit, and Hermiati, Euis
- Subjects
- *
XYLOSE , *XYLITOL , *SUGARCANE , *MALEIC acid , *SUGAR alcohols , *WASTE management , *LIGNOCELLULOSE , *YEAST - Abstract
Xylitol is a common sugar replacement used throughout food and pharmaceutical applications as well as a component in a wide range of chemical products. Xylitol could be produced from lignocellulosic biomass through acid or enzymatic hydrolysis, continued by fermentation of the xylose-rich hydrolysate using yeasts. To obtain better results during the fermentation of acid hydrolysate, the yeast should have a good tolerance to acidic environments. For improving the tolerance of Meyerozyma caribbica Y67 to low pH conditions, we suggested a short-term adaptation technique in this work to enhance xylitol production on sugarcane trash hemicellulosic hydrolysate (STHH). This study aims to evaluate the effectiveness of short-term adaptation techniques and various pH in simple shake flasks for Meyerozyma caribbica Y67 yeast for obtaining the higher xylitol. The hydrolysate was prepared by dilute acid hydrolysis using 1.8% (v/v) maleic acid assisted by a microwave digester (1:10 solid/liquid ratio, 180 °C, 5 min, 195 rpm). Yeast was cultivated in YPX broth (pH 5.0) for 24 h to obtain inoculum cells and consecutively transferred to the subsequent YPX broth with variations in pH adjustment (4; 3.5; 3; 2.5) and each was adapted for 72 h. Adapted cells were used as inoculum in fermentations using STHH for 72-h at the same pH as that they were adapted to. Results of the study show that the adapted yeast at pH 2.5 produced the highest xylitol concentration, (0.48 g/L) with a yield (Yp/s) of 0.05 g/g xylose in 72 h compare to other adaptation conditions. Therefore, the short-term adaptation of yeast cells in STHH was demonstrated to be an effective method for enhancing yeast tolerance to the low pH of the acid hydrolysate as well as its fermentative performance. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
34. Preliminary study on hydrolysis of sugarcane trash hemicellulose by inorganic salt catalyst for xylose production.
- Author
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Hermiati, Euis, Pramasari, Dwi Ajias, and Lianawati, Adetya
- Subjects
- *
HEMICELLULOSE , *XYLOSE , *CATALYSTS , *ACID catalysts , *WASTE management , *HYDROLYSIS , *OXALIC acid , *SUGARCANE - Abstract
Sugarcane trash is abundant underutilized biomass that has high hemicellulose content as a source of xylose. Inorganic salts, such as Al2(SO4)3 and FeCl3 could act as acids and hydrolyze biomass components, especially hemicellulose. The objectives of this study were to explore the use of different amounts of the two catalysts in the hydrolysis of sugarcane trash hemicellulose using two heating methods, an autoclave or microwave digester, with water or 1% oxalic acid as a medium. The amount of catalyst was 50 and 100 µmol/ g biomass, while the heat treatment was at 121 °C for 30 min in the autoclave or 180 °C for 5 min in the microwave digester. Results of the study show that the use of microwave digester with a catalyst of 100 µmol /g biomass in an acid medium resulted in the highest biomass solubilization (40.2-41.2%). The highest reducing sugar concentration (28.1-29.8 g/L) was obtained using an autoclave or microwave digester with an acid medium and FeCl3 catalyst (100 µmol /g biomass). However, if water medium is used, the highest solubilization and reducing sugar concentrations (24.1% and 15.6 g/L, respectively) are those from the process with Al2(SO4)3 catalyst (100 µmol /g biomass). The addition of catalysts to an acid medium does not increase or even decrease xylose concentration. Meanwhile, the addition of Al2(SO4)3 catalyst (100 µmol /g biomass) could increase xylose concentration from 0.1 to 2.4 g/L and xylooligosaccharide concentration from 1.4 to 6.3 g/L when the hydrolysis is conducted in water medium using a microwave digester. Hydrolysis using Al2(SO4)3 catalyst in a water medium is more promising as an alternative to acid hydrolysis in xylose production than using FeCl3 catalyst. The higher amount of catalyst resulted in higher biomass solubilization, reducing sugar and xylose concentrations in the hydrolysate. Processes using microwave digester give better results of hydrolysis, including xylose recovery. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
35. Bioethanol production by immobilized co-culture of Saccharomyces cerevisiae and Scheffersomyces stipitis in a novel continuous 3D printing microbioreactor
- Author
-
PEDRO HENRIQUE F. RODRIGUES, ELIZABETH G. DA SILVA, ALEX S. BORGES, GABRIEL LUIS CASTIGLIONI, CARLOS ALBERTO G. SUAREZ, and INTI DORACI C. MONTANO
- Subjects
hemicellulose ,xylose ,xylulose ,biocatalyst ,ethanol ,continuous fermentation ,Science - Abstract
Abstract Biorefineries require low-cost production processes, low waste generation and equipment that can be used not only for a single process, but for the manufacture of several products. In this context, in this research a continuous 3D printing microbioreactor coupled to an Arduino-controlled automatic feeding system was developed for the intensification of the ethanol production process from xylose/xylulose (3:1), using a new biocatalyst containing the co-culture of Scheffersomyces stipitis and Saccharomyces cerevisiae (50/50). Initially, batch fermentations of monocultures of S. cerevisiae and S. stipitis and co-culture were carried out. Subsequently, the immobilized co-culture was used as a biocatalyst in continuous fermentations using the developed microreactor. Fermentations carried out in the microbioreactor presented a 2-fold increase in the ethanol concentration and a 3-fold increase in productivity when compared to monocultures. The microbioreactor developed proved to be efficient and can be extended for other bioproducts production. This approach proved to be a promising alternative for the use of the hemicellulose fraction of biomasses without the need to use modified strains.
- Published
- 2024
- Full Text
- View/download PDF
36. Studies on effective catalytic conversion of xylose to furfural using green sulfonated carbon catalysts: Process optimization by Taguchi approach
- Author
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Adeyinka Sikiru Yusuff and Yanlong Gu
- Subjects
Xylose ,Carbon-based acid catalyst ,Furfural ,Dehydration ,Optimization ,Sulfonation ,Chemistry ,QD1-999 - Abstract
Herein, furfural was synthesized via dehydration of xylose with γ-valerolactone (GVL) in the presence of sulfonated carbon catalysts prepared from eucalyptus activated carbon (EAC) and sulfonating agents (H2SO4 and TsOH). Influences of pure and mixed sulfonating agents on physicochemical attributes of the obtained sulfonated carbon catalysts were explored using different characterization techniques (BET, FTIR, NH3-TPD, CHNS, XRD, TGA, SEM and total acid density). The process input variables (temperature, solvent/substrate ratio, time and catalyst loading) influencing the dehydration process were optimized using Taguchi design approach. The best H2SO4 (H)/TsOH (T) molar ratio for the mixed acid sulfonated EAC (EAC-H-T) material to catalyze xylose conversion to furfural was 3:2. The total acid density (0.79 ± 0.08 mmol/g), specific surface area (711.9 m2/g) and sulfur concentration (9.77%) of the EAC-3H-2T catalyst were higher compared to other sulfonated EAC materials. Taguchi optimization approach revealed that highest furfural yield (74.61 ± 0.05 %) was achieved at the optimum conditions of 180 °C dehydration temperature, 1.5 wt% catalyst loading, 3.0 h dehydration time and 3 mL/g GVL/xylose ratio. 1H- and 13C NMR analyses conducted on isolated product obtained under optimum conditions confirmed the formation of furfural. In addition, the EAC-3H-2T catalyst exhibited sustained activity after it was reused for six times.
- Published
- 2024
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- View/download PDF
37. Modification of Pt/SiO2 with Mg(OH)2 Improves Xylose to Xylulose Isomerization
- Author
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Li, Wenxuan, Chen, Ming, Song, Yuanbo, Jin, Mengyu, Bi, Dongsu, Zhang, Yalei, and Shen, Zheng
- Published
- 2024
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38. Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli
- Author
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Ping Li, Mengjiao Wang, Haiyan Di, Qihang Du, Yipeng Zhang, Xiaoxu Tan, Ping Xu, Chao Gao, Tianyi Jiang, Chuanjuan Lü, and Cuiqing Ma
- Subjects
Corn cob hydrolysate ,1,2,4-butanetriol ,Xylose ,Metabolic engineering ,Escherichia coli ,Microbiology ,QR1-502 - Abstract
Abstract Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production.
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- 2024
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39. Engineering the substrate preference of glucose oxidase for the enzymatic oxidation of xylose.
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Wang, Yue, Cao, Xueting, Jiang, Shanshan, Gao, Liwei, Han, Xiaolong, Qu, Jingyao, Jiang, Xukai, Liu, Guodong, and Qu, Yinbo
- Subjects
- *
XYLOSE , *GLUCOSE oxidase , *MOLECULAR dynamics , *LIGNOCELLULOSE , *OXIDATION , *ASPERGILLUS niger , *HYDROXYL group - Abstract
Glucose oxidase (GOx) catalyzes the oxidation of D -glucose to D -glucono-1,5-lactone and has a wide range of applications in various industries. However, the strict substrate specificity of GOx hampers its application in the conversion of other abundant sugars such as D -xylose. In this study, the substrate preference of GOx from Aspergillus niger (AnGOx) was engineered using a semi-rational design approach. The mutant T110V/F414L exhibited a 5.7-fold increase in D -xylose oxidation activity compared to that of the wild-type enzyme, which was attributed to its enhanced affinity for the substrate. Molecular dynamics simulations indicated that the T110V and F414L mutations may mitigate the non-productive binding of D -xylose at the entrance of the substrate-binding pocket, and therefore, are beneficial for providing access of its C1 hydroxyl group to the catalytic residues. Moreover, the mutant simultaneously oxidized D -xylose and D -glucose in the corncob hydrolysate to the corresponding aldonic acids when coupled with catalase. These findings provide new insights into substrate recognition by GOx and offer a new method for the utilization of D -xylose from lignocellulosic feedstocks. [ABSTRACT FROM AUTHOR]
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- 2024
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40. A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production.
- Author
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Kham, Nang Nwet Noon, Phovisay, Somsay, Unban, Kridsada, Kanpiengjai, Apinun, Saenjum, Chalermpong, Lumyong, Saisamorn, Shetty, Kalidas, and Khanongnuch, Chartchai
- Subjects
- *
YEAST extract , *XYLANS , *YEAST , *SEQUENCE analysis , *XYLOSE , *TANNINS - Abstract
This study aims to utilize the microbial resources found within Laphet-so, a traditional fermented tea in Myanmar. A total of 18 isolates of thermotolerant yeasts were obtained from eight samples of Laphet-so collected from southern Shan state, Myanmar. All isolates demonstrated the tannin tolerance, and six isolates were resistant to 5% (w/v) tannin concentration. All 18 isolates were capable of carboxy-methyl cellulose (CMC) degrading, but only the isolate DK showed ethanol production at 45 °C noticed by gas formation. This ethanol producing yeast was identified to be Cyberlindnera rhodanensis based on the sequence analysis of the D1/D2 domain on rRNA gene. C. rhodanensis DK produced 1.70 ± 0.01 U of thermostable extracellular β-glucosidase when cultured at 37 °C for 24 h using 0.5% (w/v) CMC as a carbon source. The best two carbon sources for extracellular β-glucosidase production were found to be either xylose or xylan, with β-glucosidase activity of 3.07–3.08 U/mL when the yeast was cultivated in the yeast malt extract (YM) broth containing either 1% (w/v) xylose or xylan as a sole carbon source at 37 °C for 48 h. The optimal medium compositions for enzyme production predicted by Plackett–Burman design and central composite design (CCD) was composed of yeast extract 5.83 g/L, peptone 10.81 g/L and xylose 20.20 g/L, resulting in a production of 7.96 U/mL, while the medium composed (g/L) of yeast extract 5.79, peptone 13.68 and xylan 20.16 gave 9.45 ± 0.03 U/mL for 48 h cultivation at 37 °C. Crude β-glucosidase exhibited a remarkable stability of 100%, 88% and 75% stable for 3 h at 35, 45 and 55 °C, respectively. [ABSTRACT FROM AUTHOR]
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- 2024
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41. Metabolic and Bioprocess Engineering of Clostridium tyrobutyricum for Butyl Butyrate Production on Xylose and Shrimp Shell Waste.
- Author
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Wang, Hao, Chen, Yingli, Yang, Zhihan, Deng, Haijun, Liu, Yiran, Wei, Ping, Zhu, Zhengming, and Jiang, Ling
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BUTYRIC acid ,BIOCHEMICAL engineering ,AMINO acid metabolism ,BUTYRATES ,SHORT-chain fatty acids ,XYLOSE - Abstract
Microbial conversion of agri-food waste to valuable compounds offers a sustainable route to develop the bioeconomy and contribute to sustainable biorefinery. Clostridium tyrobutyricum displays a series of native traits suitable for high productivity conversion of agri-food waste, which make it a promising host for the production of various compounds, such as the short-chain fatty acids and their derivative esters products. In this study, a butanol synthetic pathway was constructed in C. tyrobutyricum, and then efficient butyl butyrate production through in situ esterification was achieved by the supplementation of lipase into the fermentation. The butyryl-CoA/acyl-CoA transferase (cat1) was overexpressed to balance the ratio between precursors butyrate and butanol. Then, a suitable fermentation medium for butyl butyrate production was obtained with xylose as the sole carbon source and shrimp shell waste as the sole nitrogen source. Ultimately, 5.9 g/L of butyl butyrate with a selectivity of 100%, and a productivity of 0.03 g/L·h was achieved under xylose and shrimp shell waste with batch fermentation in a 5 L bioreactor. Transcriptome analyses exhibited an increase in the expression of genes related to the xylose metabolism, nitrogen metabolism, and amino acid metabolism and transport, which reveal the mechanism for the synergistic utilization of xylose and shrimp shell waste. This study presents a novel approach for utilizing xylose and shrimp shell waste to produce butyl butyrate by using an anaerobic fermentative platform based on C. tyrobutyricum. This innovative fermentation medium could save the cost of nitrogen sources (~97%) and open up possibilities for converting agri-food waste into other high-value products. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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42. Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose.
- Author
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Dvořák, Pavel, Burýšková, Barbora, Popelářová, Barbora, Ebert, Birgitta E., Botka, Tibor, Bujdoš, Dalimil, Sánchez-Pascuala, Alberto, Schöttler, Hannah, Hayen, Heiko, de Lorenzo, Víctor, Blank, Lars M., and Benešík, Martin
- Subjects
PSEUDOMONAS putida ,XYLOSE ,BIOLOGICAL evolution ,PENTOSE phosphate pathway ,BACTERIAL adaptation ,GENETIC engineering ,GLYCOLYSIS ,PHYSIOLOGICAL adaptation - Abstract
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by rational metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous xylose isomerase pathway remained unresolved. Here, we elucidate the xylose metabolism and establish a foundation for further engineering followed by ALE. First, native glycolysis is derepressed by deleting the local transcriptional regulator gene hexR. We then enhance the pentose phosphate pathway by implanting exogenous transketolase and transaldolase into two lag-shortened strains and allow ALE to finetune the rewired metabolism. Subsequent multilevel analysis and reverse engineering provide detailed insights into the parallel paths of bacterial adaptation to the non-native carbon source, highlighting the enhanced expression of transaldolase and xylose isomerase along with derepressed glycolysis as key events during the process. Pseudomonas putida is becoming a host of choice for the valorization of lignocellulosic substrates. Here, the authors provide insight into the adaptation of this bacterium to the non-native substrate D-xylose, enabled by metabolic engineering and adaptive laboratory evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Promising sulfonated carbon-based zirconia catalyst for renewable furfural production.
- Author
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Ogundowo, Oluwafadeyinmi and Ibrahim, Hussameldin
- Abstract
Catalytic conversion of xylose and waste biomass to furfural is important in augmenting the existing supply of fossil-fuel-derived fuels and chemicals. Here, thermal treatment was used to successfully generate a sulfonated carbonaceous zirconia catalyst comprising bio renewable glucose, p-toluene sulfonic acid, and zirconium isopropoxide. This solid acid catalyst was used for heterogeneous catalytic hydrolysis of biomass and xylose dehydration to furfural. Using N
2 -physisorption, NH3 -temperature-programmed desorption (NH3 -TPD), X-ray diffraction (XRD), Fourier transform infrared (FTIR), and elemental analysis, the acidity, porosity, presence, and influence of the sulfonic acid group and zirconium were studied. The performance of the catalyst for xylose dehydration and lignocellulosic biomass transformation to furfural was examined. This heterogeneous catalyst demonstrated good catalytic activity in the manufacture of furfural, resulting in a 40% increase in furfural yield from flax straw biomass at 190 °C after 120 min. This heterogeneous catalyst also allowed for xylose dehydration to furfural without the formation of degradation products, and a recyclability investigation revealed that the catalyst was relatively stable, with only a 5% loss in furfural yield. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
44. Efficient photocatalytic conversion of xylose to co-produce xylonic acid and CO via a dual S-scheme heterojunction photocatalyst between carbon nitride and CuInS2 quantum dot-sensitized ZnIn2S4.
- Author
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Liu, Kangning, Zhang, Junqiang, Ma, Jiliang, and Sun, Runcang
- Subjects
- *
HETEROJUNCTIONS , *QUANTUM dots , *NITRIDES , *XYLOSE , *LIGHT absorption - Abstract
Heterojunction photocatalysts receive significant interest due to their high performance and easy fabrication. An S-scheme heterojunction is developed on the basis of conventional type II heterojunctions, which can further promote charge separation and migration. In this work, a photocatalyst with a dual S-scheme heterojunction (denoted as C-s-ZIS/CN) is developed by the hybridization of CuInS2 quantum dot-sensitized ZnIn2S4 nanosheets and g-C3N4 nanosheets. CuInS2 quantum dot sensitization efficiently enhanced light absorption. Meanwhile, the unique charge migration pathway in the dual S-scheme heterojunction accelerated the separation and transfer of photogenerated carriers. The photocatalyst was innovatively employed in the simultaneous photocatalytic production of xylonic acid and CO. Under 10 W LED light irradiation (790 mW cm−2), the CO evolution rate reached a high of 228.94 μmol g−1 h−1. When a xenon lamp was employed, the CO evolution rate increased to 964.27 μmol g−1 h−1. Moreover, the optimal xylonic acid yield achieved was up to 58.56%. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
45. Efficient photocatalytic conversion of xylose to co-produce xylonic acid and CO via a dual S-scheme heterojunction photocatalyst between carbon nitride and CuInS2 quantum dot-sensitized ZnIn2S4.
- Author
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Liu, Kangning, Zhang, Junqiang, Ma, Jiliang, and Sun, Runcang
- Subjects
HETEROJUNCTIONS ,QUANTUM dots ,NITRIDES ,XYLOSE ,LIGHT absorption - Abstract
Heterojunction photocatalysts receive significant interest due to their high performance and easy fabrication. An S-scheme heterojunction is developed on the basis of conventional type II heterojunctions, which can further promote charge separation and migration. In this work, a photocatalyst with a dual S-scheme heterojunction (denoted as C-s-ZIS/CN) is developed by the hybridization of CuInS
2 quantum dot-sensitized ZnIn2 S4 nanosheets and g-C3 N4 nanosheets. CuInS2 quantum dot sensitization efficiently enhanced light absorption. Meanwhile, the unique charge migration pathway in the dual S-scheme heterojunction accelerated the separation and transfer of photogenerated carriers. The photocatalyst was innovatively employed in the simultaneous photocatalytic production of xylonic acid and CO. Under 10 W LED light irradiation (790 mW cm−2 ), the CO evolution rate reached a high of 228.94 μmol g−1 h−1 . When a xenon lamp was employed, the CO evolution rate increased to 964.27 μmol g−1 h−1 . Moreover, the optimal xylonic acid yield achieved was up to 58.56%. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
46. High-Efficient Hydrogenolysis of Xylose to Polyols Over Ni-W/CeO2 Catalysts.
- Author
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Zhu, Peng, Han, Chunyang, and Xia, Haian
- Subjects
- *
POLYOLS , *HYDROGENOLYSIS , *XYLOSE , *GLYCOLS , *ETHYLENE glycol , *CATALYSTS , *XYLANS , *TRANSMISSION electron microscopy - Abstract
Catalytic hydrogenolysis of xylose to ethylene glycol (EG) and 1,2-propanediol (1,2-PG) is an important way for the high-value valorization of biomass. Herein, Ni-W/CeO2 catalysts prepared by incipient-wetness impregnation method were used for the hydrogenolysis of xylose to EG and 1,2-PG. Several characterizations such as X-ray diffraction (XRD), Transmission electron microscopy (TEM) and X-ray photoelectronic spectroscopy (XPS) were used to analyze the physicochemical properties of the catalysts. The effects of different Ni/W ratios and reaction conditions on the hydrogenolysis performance of the catalyst were investigated. The doping of W promoted the selective cleavage of C–C and C-O bonds of xylose, thus improved the yield of EG 1,2-PG. The doping of W can form interactions with Ni and increase the oxygen vacancy concentration of CeO2, which have a significant impact on the catalyst activity. When the Ni: W ratio is around 1:1, the catalyst has the best activity, and the total yield of diols can reach about 43.2%.. When xylan was used as the substrate, the total yield of diols can reach to 58.7%. The possible reaction mechanism of xylose hydrogenolysis was also discussed. This work developed a new catalyst system for the directional conversion of xylose to EG and 1,2-PG, which provided a new idea for the design of catalyst to converting lignocellulose, especially carbohydrate into high-value chemicals. Ni-W/CeO2 with different Ni/W ratio are efficient catalysts for hydrogenolysis of xylose to EG and 1,2-PG. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. Production and Characterization of bacterial cellulose from.
- Author
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DOĞAN, Nazime MERCAN, TOP, Burak, BOZBEYOĞLU, Naime Nur, BULUT, Duygu TAKANOĞLU, KARABULUT, Orhan, and UĞUZDOĞAN, Erdal
- Subjects
- *
SCANNING electron microscopes , *ELECTRIC conductivity , *XYLOSE , *CELLULOSE , *SURFACE area , *LACTOSE , *ARABINOSE - Abstract
In this study, bacterial cellulose (BC) was obtained from Komagataeibacter xylinus S4 and characterized in detail. The effects of a various of carbon sources and media, different pH conditions, incubation temperatures, Surface area/Volume ratios, and incubation durations were determined for BC production. Considering the carbon types, the amount of BC production from high to low was realized as sucrose, fructose, mannitol, xylose, arabinose, and lactose. The highest BC amount (1.303 g/L) was achieved by combining M1A05P5 broth, 30 °C, 1.06 cm-1 Surface area/Volume ratio, pH 3.5 and 21 days. According to scanning electron microscope (SEM) analysis, the cellulose fibril diameters were 34.87-45.97 nm at pH 3.5 and 29.71-102.3 nm at pH 6.5 in M1A05P5. Also, TGA analysis exhibited that the weight loss of BC in the removal of water step initialized between 50 °C and 150 °C and the degradation step initialized between 215 °C and 228 °C. Finally, the electrical conductivity values of the BC samples were determined on the 27-137 °C temperature scale. It was observed that the conductivity was temperature dependent, and the conductivity increased exponentially as the temperature increased. In conclusion, the cellulose from K. xylinus S4 typically showed a semiconducting behavior. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
48. Xylose, Glucose and Fructose Extraction from Different Herbaceous Crops in a Green Biorefinery Demonstration Platform: a Comparative Study.
- Author
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Lopez Fetzer, Damian E., Andrade, Thalles A., and Ambye-Jensen, Morten
- Subjects
XYLOSE ,FRUCTOSE ,HERBACEOUS plants ,EXTRACTION (Chemistry) ,FERMENTATION - Abstract
The extraction of xylose, glucose, fructose, and other sugars in different brown juice (BJ) biomasses in a green biorefinery may be used on a large scale for fermentation and biobased production. A comparative study of different BJ obtained in a biorefinery demonstration platform was conducted employing white clover, red clover, grass-clover mixture, alfalfa, festulolium, and ryegrass as feedstock. In the green biorefinery, the biomass was submitted to mechanica wetl fractionation in which a green juice (GJ) and a fiber cake fraction were separated. The protein was extracted from the GJ by using heat precipitation at 85 ˚C and, sent to a decanter centrifugation, where a leaf protein concentrate was separated from the BJ. Total sugar content varied between 29.10 g/L to 10.82 g/L, in BJ from the different feedstocks. Glucose constitutes the carbohydrate with the highest concentration in BJ of festulolium, ryegrass, red clover, and grass-clover feedstock. Among all the remaining carbohydrates in the BJ, xylose is the major sugar in BJ from alfalfa and white clover. However, the highest xylose concentration was in red clover with 7.04 g/L. Ryegrass showed a higher concentration of fructose with 9.17 g/L. In general, fructose was extracted in all the BJ biomasses with quantities up to 4.73 g/L, except alfalfa with 1.81 g/L. Ryegrass and festulolium exhibited a majority concentration with 5.19 and 8.43 g/L of sucrose. Maltose and galactose presented low sugar concentrations and arabinose had the lower concentrations in BJ biomasses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
49. On the succinic acid production from xylose by growing and resting cells of Actinobacillus succinogenes: a comparison.
- Author
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Escanciano, Itziar A., Ladero, Miguel, and Santos, Victoria E.
- Abstract
Succinic acid is a key platform chemical in modern biochemical manufacturing. Optimization of the production of succinic acid production in terms of product titers and economy of resources is of vital importance. In this work, we have studied the succinic acid production by using both produced using growing and resting cells of Actinobacillus succinogenes. First, the process catalyzed by resting cells was enabled by the development of a two-step inoculum strategy which was crucial for the bacterial adaptation to the carbon source. The process was performed in the absence of nitrogen source, disabling cell duplication, and restricting endogenous respiration. While the product yields were almost identical when using growing cells (0.44 g‧g
−1 ) and cells in resting state (0.43 g‧g−1 ); very interestingly, the by-product formation was dramatically reduced when operating with resting cells. Next, the format of resting cells was studied; biofilms were found to be more active than cells in suspension, in terms of specific activity, but the lower cellular concentration in biofilms affected negatively the acid final titers. Finally, a complete and simplified kinetic model was proposed and successfully fitted to the relevant retrieved data of biomass, substrate, products, and by-products both in production with growing and resting cells. These results pave the way for the optimization of succinic acid production processes with reduced nitrogen source consumption, promoting a higher selectivity to the target acid, which facilitates the subsequent downstream separation operations. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
50. Studying the Effect of Torrefaction on the Carbohydrate Components of Birch and Pine Wood.
- Author
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Pushkin, S. A., Grachev, A. N., Makarov, A. A., Khaziakhmedova, R. M., Bashkirov, V. N., and Zabelkin, S. A.
- Abstract
The results of studies of the carbohydrate components of birch and pine wood samples torrefied in the temperature range of 200–300°C are presented. An analysis of the monosaccharide composition of carbohydrates hydrolyzed by trifluoroacetic acid has been performed. Resulting from torrefaction, the level of all the monosaccharides except for glucose exhibits a decrease. Already at 200°C, the amount of xylose extracted with the use of trifluoroacetic acid decreases by approximately two times as to compare to the reference sample. Increasing the torrefaction temperature to 250°C results in a decrease in the level of extractable xylose to 1% of the dry weight of initial material. The difference between changes in the monosaccharide composition of pine and birch cell walls is represented by the dynamics of the level of extracted glucose. Owing to torrefaction, a decrease in the amount of carbohydrate components occurs and, consequently, a decrease in the biodegradability of wood is observed, which should be taken into account in the course of composite materials development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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