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Your search keyword '"Tsoumani, Marina"' showing total 2 results
2 results on '"Tsoumani, Marina"'

1. Mast cell activation test in the diagnosis of allergic disease and anaphylaxis.

Author

Bahri R, Custovic A, Korosec P, Tsoumani M, Barron M, Wu J, Sayers R, Weimann A, Ruiz-Garcia M, Patel N, Robb A, Shamji MH, Fontanella S, Silar M, Mills ENC, Simpson A, Turner PJ, and Bulfone-Paus S

Subjects
Adolescent, Adult, Allergens immunology, Arachis immunology, Basophil Degranulation Test, Cell Degranulation, Cells, Cultured, Child, Cohort Studies, Female, Humans, Immunoglobulin E metabolism, Male, Young Adult, Anaphylaxis diagnosis, Immunologic Tests methods, Mast Cells physiology, Peanut Hypersensitivity diagnosis
Abstract

Background: Food allergy is an increasing public health issue and the most common cause of life-threatening anaphylactic reactions. Conventional allergy tests assess for the presence of allergen-specific IgE, significantly overestimating the rate of true clinical allergy and resulting in overdiagnosis and adverse effect on health-related quality of life., Objective: To undertake initial validation and assessment of a novel diagnostic tool, we used the mast cell activation test (MAT)., Methods: Primary human blood-derived mast cells (MCs) were generated from peripheral blood precursors, sensitized with patients' sera, and then incubated with allergen. MC degranulation was assessed by means of flow cytometry and mediator release. We compared the diagnostic performance of MATs with that of existing diagnostic tools to assess in a cohort of peanut-sensitized subjects undergoing double-blind, placebo-controlled challenge., Results: Human blood-derived MCs sensitized with sera from patients with peanut, grass pollen, and Hymenoptera (wasp venom) allergy demonstrated allergen-specific and dose-dependent degranulation, as determined based on both expression of surface activation markers (CD63 and CD107a) and functional assays (prostaglandin D 2 and β-hexosaminidase release). In this cohort of peanut-sensitized subjects, the MAT was found to have superior discrimination performance compared with other testing modalities, including component-resolved diagnostics and basophil activation tests. Using functional principle component analysis, we identified 5 clusters or patterns of reactivity in the resulting dose-response curves, which at preliminary analysis corresponded to the reaction phenotypes seen at challenge., Conclusion: The MAT is a robust tool that can confer superior diagnostic performance compared with existing allergy diagnostics and might be useful to explore differences in effector cell function between basophils and MCs during allergic reactions., (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2018
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2. Mast cell activation test in the diagnosis of allergic disease and anaphylaxis

Author

Bahri, Rajia, Custovic, Adnan, Korošec, Peter, Tsoumani, Marina, Barron, Martin, Wu, Jiakai, Sayers, Rebekah, Weimann, Alf, Ruiz-Garcia, Monica, Patel, Nandinee, and Šilar, Mira

Subjects
food hypersensitivity, immunologic tests, BAT, udc:616-097, mast cells, mast cell activation test, anafilaksija, alergija in imunologija -- diagnostika, allergy and immunology -- diagnosis, basophil activation test, preobčutljivost za hrano, test aktivacije bazofilcev, test aktivacije mastocitov, anaphylaxis, imunološki testi, mastociti
Abstract

Background. Food allergy is an increasing public health issue and the commonest cause of life-threatening anaphylactic reactions. Conventional allergy tests assess for the presence of allergen-specific IgE, significantly overestimating the rate of true clinical allergy resulting in over-diagnosis and adverse impact on health-related quality of life. Objective. To undertake initial validation and assessment of a novel diagnostic tool, the mast cell activation test (MAT). Methods. Primary human mast cells (hMCs) were generated from peripheral blood precursors, and sensitized using patient sera and then incubated with allergen. Mast cell degranulation was assessed by flow cytometry and mediator release. We compared the diagnostic performance of MAT to existing diagnostic tools to assess in a cohort of peanut-sensitized individuals undergoing double-blind, placebo-controlled challenge. Results. hMCs sensitized with sera from peanut, grass pollen and hymenoptera- (wasp venom) allergic patients demonstrated allergen-specific and dose-dependent degranulation by both expression of surface activation markers (CD63 and CD107a) and functional assays (prostaglandins D2 and ß-hexosaminidase release). In this cohort of peanut-sensitized individuals, MAT was found to have superior discrimination performance compared to other testing modalities including component-resolved diagnostics and basophil activation test. Using functional principle component analysis, we identified 5 clusters or patterns of reactivity in the resulting dose-response curves, which at preliminary analysis corresponded to the reaction phenotypes seen at challenge. Conclusion. MAT is a robust tool which may confer superior diagnostic performance compared to existing allergy diagnostics, and may be useful to explore differences in effector cell function between basophils and mast cells during allergic reactions.

Published
2020

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