1. Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma.
- Author
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Shih YH, Chang KW, Chen MY, Yu CC, Lin DJ, Hsia SM, Huang HL, and Shieh TM
- Subjects
- Animals, Blotting, Western, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell pathology, Cell Proliferation, Cell Transformation, Neoplastic genetics, Disease Models, Animal, Gene Expression Regulation, Neoplastic, Immunohistochemistry, In Vitro Techniques, Mice, Mice, Nude, Mouth Neoplasms genetics, Mouth Neoplasms pathology, Neoplasm Transplantation, Protein-Lysine 6-Oxidase metabolism, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Tumor Cells, Cultured, Carcinoma, Squamous Cell enzymology, Copper pharmacology, Mouth Neoplasms enzymology, Neovascularization, Pathologic genetics, Protein-Lysine 6-Oxidase genetics
- Abstract
Background: Lysyl oxidase (LOX) is a copper-dependent enzyme that cross-links collagen and elastin in the extracellular matrix. LOX overexpressed in various tumors. The manner in which LOX affects tumor growth remains controversial., Methods: Chemical treatment and gene transfection were used to induce LOX overexpression or inhibition in cell lines SAS and SVEC4-10. LOX mRNA, protein, and activity were confirmed before tube formation assay and tumorigenesis. The microvessels in the tumor section were detected by immunostaining CD31-positive endothelial cells., Results: LOX overexpression and copper induction of LOX activity increased SVEC4-10 tube formation. LOX silencing and β-aminopropionitrile inhibition of LOX activity had opposite effects. LOX overexpression increased proliferation and proliferating cell nuclear antigen expression. High LOX expression clones increased tumor size in a tumorigenesis model. The microvascular numbers were higher in LOX overexpression tumors than in control tumors., Conclusion: LOX can induce cell proliferation and angiogenesis in oral squamous cell carcinoma., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2013
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