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This article explores the possibilities of a cheap one-dollar microscope, the Foldscope, for enhancing out-of-school science education. Developed by Manu Prakash and Jim Cybulski from Stanford University, these origami-type paper microscopes make it possible to provide all students with their own microscopes, due to the low cost. This provides students the opportunity to engage in science outside of the classroom, as amateur sleuths engaged in environmental inquiries, e.g., determining the levels of pollution of local water resources. In this article the authors share two sets of research data: an activity where school students engaged in authentic problem-based learning using the Foldscopes, as well as student teachers' experiences of engaging with Foldscope microscopes. The outcomes of the first research project indicate that affective outcomes and cognitive gains were achieved. Responses in the second research project included five categories: preparation and presentation; potential of the Foldscope; use of slideshow; energy/complements; and limitations. The conclusion reached was that Foldscopes hold possibilities for enhancing STS (science-technology-society) approaches inside and outside the classroom. One recommendation is that such frugal-science approaches are emphasized more in both pre-and in-service teacher education.

The international Eco-Schools programme promotes Education for Sustainable Development (ESD) through introducing and stimulating pro-environmental initiatives by school learners and staff. This enabled learners in the Eco-Clubs at a resource-poor primary school to identify and undertake transformative pro-environmental initiatives in 2011 and 2014 through Self-Directed Education (SDE). An educative approach encouraging critical thinking at the school provided the foundation that made this possible. In discussing and working through their strategies to undertake research and challenge authorities about noncompliance in regard to municipal responsibilities that led to environmental degradation, Eco-Club members liaised freely with teachers and other learners. This process, together with local support for the eco-school initiatives, stimulated widespread interest and generated hope among learners by showing that another way of being is possible.

Manu Prakash, the developer of the foldscope microscope reported on in this paper, stated that it is important to use tools that can support open-ended inquiry in the classroom, without dumbing down those tools. Scientific equipment in the school laboratory is often very expensive and only available to those who can afford it. "Frugal science" is a trend in education that researches, develops and introduces economical and quality scientific resources to developing countries. In South Africa, many underprivileged schools lack quality practical and laboratory resources to perform simple tasks, such as microscopy. Furthermore, the absence of laboratory investigations could lead to learners not enjoying Life Sciences nor developing a more nuanced understanding of the nature (tenets) of science. As part of an indigenous knowledge intervention hosted by the North-West University, teachers were provided with $1 foldscopes (paper microscope) to use in their classrooms. This research reports on the views of Life Sciences learners and teachers on the use of foldscopes in the Life Sciences classroom during a practical lesson. The focus of the research is to illuminate how such problem-based approaches could enhance affective outcomes. This generic qualitative research study has elements of design-based research (DBR) as well as classroom action research (CAR), carried out by participating teachers to investigate the affordances of foldscopes. Data was collected using observations, teacher reflections, learner reflections, photographs and personal interviews. From an affective stance, this qualitative study used Engeström's third-generation Cultural-Historical Activity Theory (CHAT) as a research lens in order to identify factors that promote or inhibit the use of foldscopes in the Life Sciences classroom during a practical lesson.

Artificial neural networks (ANNs) are powerful data-oriented "black-box" algorithms capable of assessing and delineating linear and multifaceted non-linear correlations between the dependent and explanatory variables. Through the years, neural networks have proven to be effective and robust analytical techniques for establishing artificial intelligence-based tools for modelling, estimating, and projecting spatial and temporal variations in water bodies. Accordingly, ANN-based algorithms gained increased attention and have emerged as practical alternatives to traditional approaches for hydro-chemical analysis. ANNs are among the widely used computer systems for modelling surface water quality. Considering their wide recognition, resilience, flexibility, and accuracy, the current study employs a neural network-based methodology to construct a novel water quality index (WQI) model suitable for analysing South African rivers. The feed-forward, back-propagated multilayered perceptron model has three parallel-distributed neuron layers interconnected with seventy weighted links orientated laterally from left to right. First, the input layer includes thirteen neuro-nodes symbolising thirteen explanatory variables, including NH3, Ca, Cl, Chl-a, EC, F, CaCO3, Mg, Mn, NO3, pH, SO4, and turbidity (NTU). Second, the hidden layer consists of eleven neuro-nodes accountable for computational tasks. Lastly, the output layer features one neuron responsible for conveying network outcomes using a single-digit WQI rating extending from zero to one hundred, where zero represents substandard water quality and one hundred denotes exceptional water quality. The AI-based model was developed using water quality data obtained from six monitoring locations within four drainage basins under the management of the Umgeni Water Board in the KwaZulu-Natal Province of South Africa. The dataset comprises 416 samples randomly divided into training, testing, and validation sets using a proportional split of 70:15:15%. The Broyden–Fletcher–Goldfarb–Shanno (BFGS) technique was utilised to conduct backpropagation training and adjust synapse weights. The dependent variables are the WQI scores from the universal water quality index (UWQI) model developed specifically for South African river basins. The ANN demonstrated enhanced efficiency through an overall correlation coefficient (R) of 0.985. Furthermore, the neural network attained R-values of 0.987, 0.992, and 0.977 for the training, testing, and validation intervals. The ANN model achieved a Nash–Sutcliffe efficiency (NSE) value of 0.974 and coefficient of determination (R2) of 0.970. Sensitivity analysis provided additional validation of the preparedness and computational competence of the ANN model. The typical target-to-output error tolerance for the ANN model is 0.242, demonstrating an adequate predictive ability to deliver results comparable with the target UWQI, having the lowest and highest index ratings of 75.995 and 94.420, respectively. Accordingly, the three-layer neural network is scientifically sound, with index values and water quality evaluations corresponding to the UWQI results. The current research project seeks to document the processes used and the outcomes obtained. [ABSTRACT FROM AUTHOR]

There is a rapid spread of antibiotic resistance in the environment. However, the impact of antibiotic resistance in drinking water is relatively underexplored. Thus, this study aimed to quantify antibiotic resistance genes (ARGs) and antibiotic residues in two drinking water production facilities (NW-E and NW-C) in North West Province, South Africa and link these parameters to bacterial communities. Physicochemical and ARG levels were determined using standard procedures. Residues (antibiotics and fluconazole) and ARGs were quantified using ultra-high performance liquid chromatography (UHPLC) chemical analysis and real-time PCR, respectively. Bacterial community compositions were determined by high-throughput 16S rRNA sequencing. Data were analysed using redundancy analysis and pairwise correlation. Although some physicochemical levels were higher in treated than in raw water, drinking water in NW-E and NW-C was safe for human consumption using the South African Water Quality Guideline (SAWQG). ARGs were detected in raw and treated water. In NW-E, the concentrations of ARGs (sul1, intl1, EBC, FOX, ACC and DHA) were higher in treated water than in raw water. Regarding antimicrobial agents, antibiotic and fluconazole concentrations were higher in raw than in treated water. However, in NW-C, trimethoprim concentrations were higher in raw than in treated water. Redundancy analysis showed that bacterial communities were not significantly correlated (Monte Carlo simulations, p-value >0.05) with environmental factors. However, pairwise correlation showed significant differences (p-value <0.05) for Armatimonas, CL500-29 marine group, Clade III, Dickeya and Zymomonas genera with environmental factors. The presence of ARGs and antibiotic residues in the current study indicated that antibiotic resistance is not only a clinical phenomenon but also in environmental settings, particularly in drinking water niches. Consumption of NW-E and NW-C treated water may facilitate the spread of antibiotic resistance among consumers. Thus, regulating and monitoring ARGs and antibiotic residues in drinking water production facilities should be regarded as paramount. [ABSTRACT FROM AUTHOR]

This study reviews worldwide wetland park research from 1996 to 2022. A bibliometric analysis is conducted on 591 wetland park studies indexed in the Web of Science and Scopus databases. The study utilizes CiteSpace and VOSviewer tools to visualize and explore influential research focuses, themes, directions, and countries. The citation burst indicates that from 1996 to 2022, research on wetland parks transited from exploring basic community structures to complex ecosystem service assessments and the formulation of management strategies. Furthermore, over the past three years, wetland park research has seen a significant surge in studies investigating water quality, ecosystem services, and spatiotemporal analysis. Notably, the three most frequent keywords in research on wetland parks were China, South Africa, and biodiversity. These keywords reflect regions that enhance biodiversity via wetland parks. The spectral-clustering algorithm identifies carbon sequestration as a research focus, highlighting the vital role of wetlands in the carbon cycle. Most authors work in developed countries' institutions, but some are from developing countries like China, South Africa, and India. The findings suggest that economic development is crucial in wetland park construction and significantly influences related research. Developed countries may offer more PhD positions to developing countries' researchers in the field and raise their awareness about wetland conservation. Given the holistic requirements of wetlands, this research recommends that educators should adopt an interdisciplinary approach in the future when nurturing wetland staff. Additionally, the study maps out the primary areas of interest in wetland park research, including environmental science, ecological economics, forestry, wetlands, tourism, and management. New artificial intelligence and digital technologies should be developed for wetland park research. This study fills a research gap: quantitative and visualized knowledge-mapping and bibliometrics on wetland parks are scarce. Additionally, no previous study has explored the relationship between wetland park research and the economic development of countries. [ABSTRACT FROM AUTHOR]

River water quality modelling relies on a good dataset for model input, calibration and validation to develop a reliable model. Determination of data requirements, and data sourcing towards this purpose in South Africa, are not elementary. Data availability is key throughout the modelling processes, making the sourcing of good data equally imperative. This paper provides starting considerations, and compiles a list of available data sources applicable to river water quality modelling in South Africa. It also provides a background on the included data sources and a general overview of the extent of the databases. [ABSTRACT FROM AUTHOR]

The Vaal Dam catchment, which is the source of potable water for Gauteng province, is characterized by diverse human activities, and the dam encounters significant nutrient input from multiple sources within its catchment. As a result, there has been a rise in Harmful Algal Blooms (HABs) within the reservoir of the dam. In this study, we employed time series analysis on nutrient data to explore the relationship between HABs, using chlorophyll-a (Chl−a) as a proxy, and nutrient levels. Additionally, Chl−a data extracted from Landsat-8 satellite images was utilized to visualize the spatial distribution of HABs in the reservoir. Our findings revealed that HAB productivity in the Vaal Dam is influenced by the levels of total phosphorus (TP) and organic nitrogen (KJEL_N), which exhibited a positive correlation with chlorophyll-a (Chl−a) concentration. Long-term analysis of Chl−a in-situ data (1986–2022) collected at a specific point within the reservoir showed an average concentration of 11.25 μg/L. However, on certain stochastic dates, Chl−a concentration spiked to very high values, reaching a maximum of 452.8 μg/L, coinciding with elevated records of TP and KJEL_N concentrations on those dates, indicating their effect on productivity levels. The decadal time series and trend analysis demonstrated an increasing trend in Chl−a productivity over the studied period, rising from 4.75 μg/L in the first decade (1990–2000) to 10.51 μg/L in the second decade (2000–2010), and reaching 16.7 μg/L in the last decade (2010–2020). The rising averages of the decadal values were associated with increasing decadal averages of its driving factors, TP from 0.1043 to 0.1096 to 0.1119 mg/L for the three decades, respectively, and KJEL_N from 0.80 mg/L in the first decade to 1.14 mg/L in the last decade. Satellite data analysis during the last decade revealed that the spatial dynamics of HABs are influenced by the dam's geometry and the levels of discharge from its two feeding rivers, with higher concentrations observed in meandering areas of the reservoir and within zones of restricted water circulation. [ABSTRACT FROM AUTHOR]

Rivers in Africa have experienced dire pollution as a result of the poor management of wastewater effluent emanating from water resource recovery facilities (WRRFs). An integrated wastewater resource recovery model was developed and applied to identify ideal wastewater resource recovery technologies that can be used to recover valuable resources from a mixture of wastewater effluents in a case study in the Burgersfort WRRF in the Limpopo province, South Africa. This novel model incorporates the process of biological nutrient removal (BNR) with an extension of conventional methods of resource recovery applicable to wastewater. The assessment of results of effluent quality from 2016 to 2022 revealed that ammonia, chemical oxygen demand, total coliform, fecal coliform, and Escherichia coli levels were critically non-compliant with the permissible effluent guidelines, indicating a stable upward trend in terms of concentrations, and scored a very bad wastewater quality index rating. All variables assessed showed a significant loading, except for orthophosphates, and significant correlations were observed among the variables. The results of the integrated wastewater resource recovery model revealed a high probability of reclaiming recoverable resources such as nutrients, sludge, bioplastics, biofuel, metals, and water from wastewater, which have economic, environmental, and social benefits, thereby improving the effluent quality of a WRRF. [ABSTRACT FROM AUTHOR]

While natural floods play a crucial role in shaping the composition of aquatic communities, the most rivers worldwide are regulated or dammed for anthropogenic purposes, resulting in alterations to the biological and chemical composition of these ecosystems. Studies have demonstrated various negative effects of river regulation on aquatic invertebrate communities globally. However, there is a scarcity of research in Africa, despite its vulnerability to anthropogenic impacts. This study aimed to compare aquatic invertebrate communities in the Phongolo River, an impacted regulated river, and the Usuthu River, a less impacted unregulated river, in South Africa. It further aimed to ascertain whether Lake Nyamithi, a naturally saline lake receiving water from both of the aforementioned systems, exhibited a stronger similarity to one of the two rivers in terms of its aquatic invertebrate composition. Aquatic invertebrate and water samples were collected from 2012 to 2018 over several surveys. The Usuthu River demonstrated a diverse and sensitive aquatic invertebrate community, emphasising its high conservation value. The Phongolo River demonstrated effects of anthropogenic impact, with taxa more resilient to changes in water quality and flow compared to the Usuthu River. Mismanagement and excessive water use may lead to the loss of any remaining sensitive aquatic invertebrate communities in this river. The presence of invasive molluscan in the Phongolo River and Lake Nyamithi also poses a threat to the native aquatic invertebrate communities. These invasive species are currently absent from the Usuthu River although other invasive species, such as the Australian redclaw crayfish, are found in both river systems. Lake Nyamithi displayed a unique aquatic invertebrate community, distinct from both rivers and their floodplains. This study provides important baseline information on the Usuthu River's aquatic invertebrates and emphasises the need to maintain adequate water flow in rivers and floodplains to protect biodiversity and sensitive species. [ABSTRACT FROM AUTHOR]

The quality of surface water has a significant impact on human health and the entire ecological system. Sewer spillages from the surrounding informal settlements discharging into the river, carrying high concentrations of fecal coliforms, are one of the major causes of extreme pollution in the rivers of South Africa. These informal settlements are common in many developing countries, and they are usually located near waterways to compensate for basic demands for water, sanitation, and recreational space, where municipal infrastructure lags behind urban growth. One major problem has been poor sanitation and poor waste disposal practices in the informal settlements, which has led to the contamination of water resources. This study aims to assess the extent to which poor sanitation in informal settlements impacts the water quality of South African rivers, given the rapid rise in population and unemployment rate. The study also highlights health and environmental issues in the local regions caused by poor sanitation. Contamination of water bodies is associated with serious health problems and fatalities. Therefore, there is a need for frequent monitoring and management of waste products discharged into the neighboring aquatic environments. [ABSTRACT FROM AUTHOR]

This study focuses on the evaluation of trace metals as well as microbial contamination of groundwater. Groundwater samples were collected from 17 boreholes. The microbial quality was tested using membrane filtration method. Higher levels of contamination for both E. coli and total coliform was recorded in the wet season. Majority of the boreholes had nitrate levels above the regulatory guideline value of the World health Organisation and the South African National Standards. The water type was established by Piper plot which showed the predominance of a magnesium bicarbonate water type, with alkaline earth metals dominating the alkali metals, as well as the weaker acids (bicarbonates) dominating the stronger ones (Sulphates and chlorides). Most of the trace metals detected were in compliance with the regulatory standard except for aluminium (0.41–0.88 mg/L). The hazard quotient and Hazard indice exceeded 1 mostly for children in both season which implies a possible non-carcinogenic health risk is associated with the continuous consumption of the water resource. The estimations of carcinogenic risk (CRing) for Cr and Pb exceeded the carcinogenic indices of 10−6 and 10−4 which could pose adverse effects on human health for both children and adults. Therefore, it is recommended that measures should be implemented to reduce the risk. [ABSTRACT FROM AUTHOR]

This study focused on assessing the extent of pollution in both flowing and non-flowing surface water within the Matjhabeng mining area of South Africa, with particular emphasis on the substantial impact of gold mine tailings. A comprehensive analysis of physical water-quality attributes, including potentially toxic elements (PTEs), and relevant pollution risk indices was undertaken. To comprehensively elucidate the potential risks to aquatic organisms and human health, a risk assessment framework predicated upon the source–pathway–receptor model was developed. Principal Component Analysis (PCA) was employed as a multivariate statistical tool to discern the potential origins of PTE contamination within the environment. The results substantiate pronounced pollution manifestations within the surface water milieu of the Matjhabeng mining area. Specifically, concentrations of critical PTEs, such as arsenic, cobalt, copper, iron, selenium, and zinc, exhibited transgressions of the regulatory thresholds stipulated by both the South African Department of Water Affairs and Forestry (DWAF) and the Canadian Council of the Ministers of the Environment (CCME). Additionally, concentrations of the aforementioned elements exceeded the stipulated DWAF guidelines for irrigation water usage. Pollution indices, encompassing the Single-Factor Pollution Index and the Nemerow Integrated Pollution Index, discerned moderate contamination stemming from As, while remarkably elevated pollution levels were identified for selenium. PCA elucidated 94.5% of the aggregate variance, revealing cobalt, copper, nickel, and zinc as coalescing within PC1, indicative of a common anthropogenic provenance that is conceivably linked to historical gold mine tailings. PC2 exhibited an aggregation of chromium, iron, and lead, reaffirming this shared anthropogenic etiology. The third PCA component was characterized by selenium, followed by arsenic and magnesium in the fourth. The resultant PTE contamination underscores a profound ecological and public health risk, impacting both the aquatic ecosystems and the local community within the precincts of the Matjhabeng Local Municipality (MLM) area, with consequential amplification of susceptibilities to deleterious health consequences. Urgent and concerted interventions are imperative to ameliorate the emergent decline in surface-water quality within the MLM locale. The adoption of nature-based remediation paradigms holds promise for efficaciously elevating water quality, ameliorating community health, and underpinning the long-term economic viability of the region. [ABSTRACT FROM AUTHOR]

A survey of the quality of water flowing from micro-system types to the ocean, along the subtropical east coast of South Africa, showed a wide variation in the concentrations of total nitrogen, phosphorus and phytoplankton biomass in the different systems located, in many cases, only a short distance from each other along the coastline. The origins of the high phytoplankton growth indicate pollutants caused by the land-use in this highly populated coastal region. The main agricultural activities in the area are sugarcane, permanent orchards, and forestry. The levels of N and P in the water varied from ‘good’ to ‘poor’, i.e., TN 0.15–3.99 mg·L−1, TP 0.02–0.33 mg·L−1 and chlorophyll-a from 0 to almost 45 µg·L−1. Rapid coastal population densification appears to have been the cause of the pollution levels measured for total nitrogen, phosphorus, and phytoplankton biomass. Most of the micro-systems with a total modified peri-catchment above 80% were enriched by both TN and TP. While the hypothesis tested was that the main cause was residential development (e.g., septic tank effluent), it was not possible to show any statistical significance to support such a specific conclusion. Although these systems are small individually, the great number along the coastline warrants recognition as important sources of freshwater inflow and nutrients to the marine environment. [ABSTRACT FROM AUTHOR]

With water quality deteriorating rapidly at a global scale, river sections suited to serve as reference sites are being increasingly lost. It thus becomes critical to develop rapid methods to confirm that previously monitored sites continue meet the requirements of reliable reference sites. In the absence of pristine sites, 9 near-natural sites, as defined by the Kleynhans (1996) classification, were used as reference sites for the Luvuvhu River catchment to compare the quality of physico-chemical factors against a biological metric. Baetid mayfly community structure at a site was chosen as an index of water quality, since this family is common in all types of freshwaters, highly diverse and adapted to unpolluted running water. Baetid larvae were sampled monthly from stones-in-current biotopes across 9 sites for over 1 year, between December 2016 and January 2018. A Spearman’s correlation test was used to evaluate the relationship between physicochemical factors and identify redundant variables. Water quality standards were measured against the national water quality guidelines for aquatic ecosystems. We used a generalized linear model to determine the effect of physico-chemical variables on baetid species, and canonical correspondence analysis to show the relationships between baetid species, sites, and physico-chemical variables. A total of 3 039 individuals belonging to 12 mayfly species were recorded. Our findings indicated that while the physico-chemical factors were highly variable, they were within favourable ranges to reflect reference site conditions. While water temperature was the most important driver of baetid community structure in general, as it negatively affected their abundances, a subset of species (Pseudoponnota sp., Pseudocloeon sp., Acanthiops varius and Demoulinia crassi) showed clear responses to changes in TDS and stream width. We conclude that specific baetid species show good potential as biological indicators of reference sites and chronic water temperature stress, making assessment of reference sites easier. [ABSTRACT FROM AUTHOR]

The present study aims at evaluating the quality of shallow groundwater (SGW) and its suitability for irrigation purpose in the most urbanised part of Johannesburg city, South Africa. The SGW samples were collected in three consecutive years and analysed for 20 selected physicochemical parameters, and heavy metals. The results were compared with the South African water quality, and Food and Agricultural Organization irrigation water quality guidelines, and standard indices derived from laboratory outputs. The results of the study show that all physiochemical parameters and heavy metals were within the limits set by both guidelines for irrigation purposes, except for potassium (3.58 mg·dm-3) and manganese levels (3.152 mg·dm-3). The calculated irrigation parameter values of sodium adsorption ratio (SAR), sodium percentage (Na%), residual sodium carbonate (RSC), magnesium hazard (MH), Kelly's ratio (KR) and permeability index (PI) were within the permissible range of irrigation water quality standards. The findings of this study provide helpful information for decision-makers such as utilisation of the studied groundwater for irrigation uses. [ABSTRACT FROM AUTHOR]

The operationalization of water resource protection initiatives for surface water resource quality and equitable water quality allocation is critical for sustainable socio-economic development. This paper assessed Blesbokspruit River Catchment's water quality status, using the South African Water Quality standards and Water Quality Index (WQI). Protection levels for quality, and waste discharge for point sources were set and evaluated using the total maximum daily loads (TMDLs) and chemical mass balance (CMB) techniques, respectively. The study found that the water quality results for the analysed physico-chemical parameters (Na+, Ca2+, Mg2+, Cl−, F−, pH, EC, SO42−) of the data collected from 2015 to 2022 were within the limits of the water quality standards, except for NO3− and PO42−. The water quality from the study area was categorized as acceptable for drinking purposes with the WQI of 54.80. The application of the TMDL approach resulted in the 77.96 mS/m for electrical conductivity (EC), 9.92 mg/L for phosphate (PO42−), and 15.16 mg/L for nitrate NO3− being set as the protection levels for the catchment. The CMB was found to be a useful tool for the evaluation of point source discharges into water resources. The study recommends the application of TMDL and CMB techniques in water resource protection practice. [ABSTRACT FROM AUTHOR]

Eutrophication-driven harmful algal blooms (HABs) can have secondary effects on larval fishes that rely on estuaries as nurseries. However, few studies worldwide have quantified these effects despite the global rise in eutrophication. This study presents a novel approach using biochemical body condition analyses to evaluate the impact of HABs on the growth and body condition of the larvae of an estuarine resident fish. Recurrent phytoplankton blooms of Heterosigma akashiwo occur in the warm-temperate Sundays Estuary on the southeast coast of South Africa. The response in body condition and assemblage structure on larval estuarine roundherring (Gilchristella aestuaria) was measured in conjunction with bloom conditions, water quality and zooplanktonic prey and predators. Larvae and early juveniles were sampled during varying intensity levels, duration and frequency of hypereutrophic blooms. This study demonstrated that extensive HABs could significantly impact larval roundherring, G. aestuaria , by decreasing larval nutritional condition and limiting their growth, resulting in poor grow-out into the juvenile phase. Poor condition and growth may likely affect recruitment success to adult populations, and since G. aestuaria is an important forage fish and zooplanktivore, poor recruitment will hold consequences for estuarine food webs. [ABSTRACT FROM AUTHOR]

There are growing concerns on contribution of vegetation dynamics to atmospheric turbidity and quality of regional water towers. The study sought to determine the trends in the MODIS/TERRA-derived normalized difference vegetation index (NDVI) and aerosol optical depth (AOD) for Lesotho Highland over 2000–2020. The predictive relationship between the two variables was also examined using regression analysis. Irrespective of yearly AOD patterns, the AOD showed biphasic patterns peaking between mid-winter to early spring (July–October) (highest) and autumn (Feb–April) (next highest), and lowest in the summer (Nov–January). The monthly NDVI was largest in January–March (summer–early fall) with smaller values in winter and spring. This seasonality can be related to the peak of anthropogenic biomass combustion during the winter and strong winds during the spring and early summer. The AOD relationship with NDVI showed quadratic patterns peaking and plunging with changes in season. About 30–80% (R2 = 0.3–0.8%) changes in annual AOD from 2000 to 2020 were explainable by the dynamics of NDVI indicating that increased NDVI contributes to about a 50% decrease in AOD in the Lesotho Highlands. However, an outlier trend was observed in 2007 (R2 = 13%). Incidences of high AOD in months of high NDVI may be indicative of traveling aerosols, i.e., aerosols from non-local sources/activity. On the other hand, high AOD in months of low NDVI implicates local aerosol sources. Trend relationship studies on vegetation loss and AOD in mountain areas of other regions could improve knowledge of contaminant dynamics and risk implications for downstream populations. [ABSTRACT FROM AUTHOR]

Water loss due to onsite leakage is a problem in the effective management of potable water mostly in semi-arid and arid countries of the world whose quantity of freshwater is depleted, and recharge is highly variable. This study assessed the quality and quantity of water loss in an academic institution due to onsite leakage resulting from various drivers such as leaks, corrosion, pressure, ageing infrastructure, and student attitude. An observational approach was used for water loss analysis. Samples were collected from each point from which water is lost and analyzed for physicochemical parameters, indicator microbes, and trace metals using standard procedures. It was observed that a high amount of water is lost in the study area which was partly influenced by high water pressure, the corrosion of taps and faucets, tap faults, and leaks. A total water loss of 9013.56 L/day in three selected residences of the institution was recorded. The wasted water was found to be of good quality with no harmful contamination. The Metal Pollution Index (MPI) showed that the water poses no threat from trace metals with a maximum MPI value of 7.76. The sampled water quality complied to the South African National Standard and World Health Organization standards for drinking water. The hazard quotient and hazard index both showed a level less than one, implying no possible non-carcinogenic risk associated with the consumption of the water. The wasted water from the institution's residences is therefore of a very high quality and needs to be conserved for better usage. [ABSTRACT FROM AUTHOR]

Freshwater ecosystems are facing degradation from human activities in the form of alterations of flow regimes, changes in land-use, and intensive water abstraction, in turn generating adverse effects on biodiversity and ecological functionality. Efficient assessment of river/stream ecosystem response to such anthropogenic changes is urgently needed. Many human activities that have an effect on water quality and river health are increasing along the Mohlapitsi River, which used to be one of the rivers with good water quality in South Africa. The aim of the study was to assess spatial variation in macroinvertebrate assemblages in relation to water quality of the Mohlapitsi River. A combination of canonical correspondence analysis and univariate analyses were used to examine the macroinvertebrate assemblages at six different sites of the Mohlapitsi River. The spatial difference in macroinvertebrate abundance was mostly related to changes in water quality. The number of taxa and the diversity of macroinvertebrates remained relatively high across the river as compared to other rivers in the Olifants River System. The midstream and downstream sites were mostly dominated by macroinvertebrates tolerant to disturbance/pollution. The most abundant family was the Thiaridae (about 60%) and mainly occurred in the midstream and downstream sites of the river (more disturbed sites). The study showed a higher evenness index in the upstream than the midstream and downstream sites which is an indication of good water quality at the upstream sites of the river. The results suggest that the river is being subjected to a moderate environmental pollution, which requires continuous assessment and monitoring to determine causes and initiate remedial measures. [ABSTRACT FROM AUTHOR]

The continuous monitoring of soil health and irrigation water quality influences the crop yield and the quality of agricultural produce. In this study, various physicochemical parameters were measured to monitor the soil profile, irrigation water and maize quality harvested from the Buhle farm located in Howick in the KwaZulu-Natal Province of South Africa to ensure steady high quality food supply for the consumer. The maize crop was sampled from the maize stalk, the corresponding soil samples were collected from the upper surface of the soil (0-15 cm surface layer) using the soil auger and the irrigation water sample was taken from the irrigation tanks using polyethylene sample bottles. The physicochemical parameters considered for irrigation water were pH, electrical conductivity, alkalinity and chloride due to their ability to affect water quality which consequently affect crop growth and quality. The soil physicochemical parameters considered were moisture content, pH, electrical conductivity, texture, total nitrogen and nutrients (protein, fat, fibre, starch, total mineral matter and elements). These parameters determine the soil quality, water content, the ratio of absorbed and lost energy, concentration of ions and elements present which in turn affect or promote the yield and quality of crops. Maize was analysed for nutritional content and medicinal health-promoting compounds to assess the influence of soil and irrigation water on the maize quality and consequently the health of the consumers. The concentrations of total nitrogen (N), phosphorus (P) and potassium (K) in soil, which were translated into high soil fertility were 2700, 19 and 222 mg kg-1, respectively. The results obtained were within the required specification for high quality maize production. The levels of sodium, sodium adsorption ratio and electrical conductivity in the irrigation water were 0.05 mg L-1, 2 and 1.81 µS m-1, respectively, indicating safe water of low salinity. Maize was high in starch (58.6%) while fibre, protein and fat contents in the maize were 23.4, 9.01 and 4.55%, respectively, indicating suitability for consumption. Furthermore, the total anthocyanin, total flavonoids and total phenolic acid content of the maize were 8.5, 49.5 and 100 mg L-1, respectively. Overall, this study showed the presence of health-promoting compounds in the maize crop which is associated with its high quality for consumption. The validity of the analysis methods was tested using certified reference materials. The concentrations of the reference materials were not statistically different from the certified values, attesting to the validity of the analysis methods. [ABSTRACT FROM AUTHOR]

Interest in greywater reuse is increasing in South Africa, because of the potential to supplement scarce freshwater resources in the face of increasing demand and aridity. This paper aims to inform the water saving-risk trade-off associated with residential untreated greywater use, through a statistical analysis of greywater quality results as sourced from prior South African studies. Greywater sources included in this review were the bathroom, kitchen, laundry, mixed and general residential sources. Variability in terms of each of the reported physical, chemical and microbiological constituents by source and between result sets was noted. Statistically significant differences were evident between the pH, conductivity and phosphorus values of certain sources. A risk assessment undertaken for each of the constituents revealed further variability. The constituent with the highest number of high-risk samples was total dissolved solids. The relatively high risk and negative consequences in greywater practices in terms of public health, the environment, and infrastructure, given this variability, provide insight into the trade-off with potential water savings. It is recommended that a more nuanced view of the potential potable savings associated with greywater reuse and also improved risk management is required by the user. [ABSTRACT FROM AUTHOR]

• Mallomonas corymbosa, M. kenyana and M. porte-ferreae were found in the Vaal River, South Africa. • Cell morphology and geographical distribution of each species are presented. • Water quality of the Vaal river at the time of sampling is presented. • The finding of M. corymbosa represents the first record of this species in Africa. • M. kenyana, a tropical/subtropical species, were found in temperate regions in South Africa. This paper reports on the presence of three Mallomonas species (Phylum Ochrophyta) in the Vaal River, South Africa. Scanning electron microscope observations of entire cells with intact scales and bristles allowed accurate identifications of M. corymbosa, M. kenyana and M. portae-ferreae. Descriptions and dimensions of each of the three species are given, their geographical distribution is discussed and physico-chemistry of the water at the time of their finding is presented. M. corymbosa was recorded for the first time in Africa, expanding its known geographical distribution to an additional continent. The climatic preferences of the tropical and subtropical species, M. kenyana were extended to temperate regions in South Africa. These species were present under eutrophic to hypertrophic conditions, a water temperature of 25.4 °C, neutral to slightly alkaline water (pH 7.25) and a conductivity value of 440 µS/cm. [ABSTRACT FROM AUTHOR]

The Hartbeespoort Dam is a discharge point of a catchment that is characterized by urbanization, mining, industrial, and agricultural activities. These activities coupled with fluxes of heavily polluted wastewater from informal settlements, wastewater treatment works, as well as runoff from golf courses have led to the development of recurring harmful algal blooms (HABs). The predominant cyanobacteria scum that is largely covering the Dam water is toxic to fish and poses serious public health risks. Phosphorus is the limiting nutrient in terrestrial aquatic systems and excess concentration in the waters usually results in eutrophication. The productivity level in Hartbeespoort Dam is also a function of total phosphorous (TP) level, showing a positive correlation with chlorophyll-a, an index for phytoplankton which are predominantly HABs in this Dam. Analysis of long-term in situ water quality data (1980–2020) show that TP is not the only driver, changes in surface water temperatures also affect the productivity level, especially, when TP levels are below a threshold of approximately 0.4 mg/L. Chlorophyll-a was retrieved from current generation high resolution satellite (Landsat and Sentinel) at 5-year interval. Standard band ratio-based ocean color model applied to satellite data produced an accuracy of R2 = 0.86 and RMSE of 5.56 µg/L. Time series analysis of in situ and satellite data show similar trends including capturing the effect of biocontrol on productivity levels between the late 1980s and the early 1990s, after which productivity increased with an increased flux of TP. Since 2015, the average annual surface temperature in the Dam has decreased leading to the decline in productivity level despite increasing levels of TP. The spatial dynamics of the HABs is a function of the discharges levels of the various rivers draining into the Dam as well as its geometry. Relatively higher concentrations are observed near river discharges and in areas of restricted water circulation. [ABSTRACT FROM AUTHOR]

The impact of treated municipal wastewater (TMW) irrigation on soil and grapevines was assessed under field conditions in vineyards in the Coastal region of South Africa. Grapevines were irrigated using TMW from the City of Cape Town uninterruptedly over a period of 11 years. Grapevines were either rain fed, irrigated with TMW via a single dripper line, or received twice the volume via double dripper lines. The quality of the TMW used for vineyard irrigation was acceptable, and below the minimum criteria stipulated by the General Authorisations to irrigate up to 500 m³ per day in terms of pH, ECw and SAR. Mean Na+ concentration in the TMW exceeded the critical value of 100 mg/ℓ for irrigating grapevines in South Africa. The Cl- levels in the TMW were well below the threshold value of 700 mg/ℓ at which toxicity in grapevines might occur. Consistently high P concentrations measured in the TMW could lead to the formation of algal blooms in water storage facilities and bio-fouling of irrigation equipment. The low N content in the TMW could not supply the annual N requirement of grapevines. The annual amount of P applied via the single dripper lines was slightly below grapevine requirements, whereas double the TMW irrigation applied excessive amounts of P. Amounts of K+ applied via TMW irrigation was in excess of annual grapevine requirements, which could affect wine quality negatively. The amount of Ca2+ and Mg2+ applied via the TMW also exceeded annual grapevine requirements. [ABSTRACT FROM AUTHOR]

Excessive discharge of poorly treated effluent has impacted global water resource systems intensely. The declining state of wastewater treatment plants (WWTPs) is a significant source of pollution in water resources. There is evidence of water resource quality deterioration in natural environments caused by effluent discharges. We assessed the impact of wastewater treatment effluent on the quality of the Crocodile River. For spatial distribution, we collected data from three WWTPs discharging effluent into the Crocodile River and from three points situated downstream of each WWTP. Physicochemical and microbiological parameters such as pH, electrical conductivity, chemical oxygen demand, phosphates, nitrates, ammonia, and Escherichia coli were analysed using standard methods of the American Public Health Association. The water quality index was also calculated to give an overall indication of pollution within the catchment. The results show that WWTPs were not complying with the effluent standards set out in their water use licence. The WWTP effluent had a negative impact on downstream water quality, with the water quality index indicating low quality of discharged effluent. It is recommended that a regular and consistent water resource quality monitoring programme be implemented, particularly in areas where effluent discharges are prevalent. In many African nations, water pollution is a serious problem that may be traced to a variety of sources. Surface water pollution has adverse effects on aquatic ecosystems and reduces the availability of clean water. In most semi-arid to dry southern African regions (e.g. South Africa), water scarcity is a significant concern. In these regions, water is a vital resource that must be protected at all times, given that the inadequate infrastructure of wastewater treatment facilities adds to the decline in South Africa's water quality standards. [ABSTRACT FROM AUTHOR]

Environmental water requirements (EWRs) are set for South Africa's estuaries to ensure that they are maintained in a state that is both achievable and commensurate with their level of conservation and economic importance. However, these EWRs are typically determined on the basis of models and scenario analyses that require extrapolation beyond existing data and experience, especially if climate change is considered. In the case of the Berg Estuary, South Africa, available data on changes in freshwater flow and water quality span a period of at least five decades (1970s--present) during which significant reduction in flows has been observed. Monitoring data also cover an extreme 3-year drought, from 2015-2017, which provided a unique opportunity to study the effects of severe freshwater starvation (zero-flow for an extended period) on this large, permanently open system. Our analyses show that mean annual runoff (MAR) under present-day conditions has been reduced to around 50% of that under reference (natural) conditions and that reduction in runoff during the low-flow season (summer) has been more severe (80-86% reduction) than for the high-flow season (39-42% reduction). The salinity gradient now extends much further upstream than under reference conditions. Hypersaline conditions along with a reverse salinity gradient were recorded in the estuary for the first time ever during the drought of 2015/17. Levels of dissolved inorganic nitrogen (NOx) reaching the estuary from the catchment have increased dramatically (6-7 fold) over the past five decades, dissolved reactive phosphate (PO4) slightly less so (2-3 fold), but ammonia (NH4) hardly at all. Increases in nutrient input from the catchment in the high-flow season are also much more dramatic than in the low-flow season. The estuary is no longer compliant with gazetted EWRs and requires urgent interventions to restore the quantity and quality of freshwater it receives. [ABSTRACT FROM AUTHOR]

The Grootspruit valley bottom wetland in South Africa, due to the impact of acid mine drainage (AMD) from an abandoned coal mine, was severely degraded before ecologically engineered interventions, as a passive treatment process, in 2014. The surface water flow of the wetland was redirected using concrete structures to enlarge the surface area of the wetland by 9.4 ha and to optimize passive treatment. Although the ecologically engineered interventions showed an improvement in water quality after the rewetting of the enlarged wetland areas, the 2016 drought had a devastating effect on the wetland's water quality. Limited natural removal of metals and sulfate concentrations by the wetland occurred during the 2016 drought, when compared with the 2015 pre-drought conditions. This period showed higher concentrations of metals, sulfate (SO42−), and electrical conductivity (EC) associated with the acidic surface water. Of particular interest was an observation of a substantial shift in pollutant-tolerant algae species in the ecologically engineered wetland outflow between the years 2015 and 2016. During the dry spell period of 2016, the diatoms Gyrosigma rautenbachiae (Cholnoky), Craticula buderi (Brebisson), and Klebsormidium acidophilum (Noris) were observed at the outflow. The latter species were not observed during the wetland surveys of 2015, before the dry spell. From late 2017 onwards, after the drought, environmental conditions started improving. In 2018, periphyton indicator species and the surface water quality were comparable to the wetland's recorded status pre-2016. The study revealed not only a regime shift, but also an ecological function loss during the drought period of 2016, followed by recovery after the dry spell. A distinct reduction in SO42−, sodium (Na), magnesium (Mg), EC, manganese (Mn), iron (Fe), silicon (Si), aluminum (Al), and pH, following the 2016 drought, highlights the utilization of water quality variables to not only assess the passive treatment responses of an ecologically engineered wetland, but also the progress relating to ecological recovery. [ABSTRACT FROM AUTHOR]

South African rivers generally receive waste from inadequate wastewater infrastructure, mines, and farming activities, among others. The uMsunduzi River in KwaZulu-Natal, South Africa, is among these recipients with recorded poor to very poor water quality. To identify parts of the uMsunduzi River that are polluted by Cryptosporidium and Escherichia coli (E. coli), this study mapped out pollutants emanating from point and non-point sources using the Soil and Water Assessment Tool (SWAT). Streamflow calibration in the upper and lower reaches of the catchment showed good performance with R² of 0.64 and 0.58, respectively. SWAT water quality output data were combined with a Quantitative Microbial Risk Assessment (QMRA) to understand the microbial health implications for people using river water for drinking, recreational swimming, and non-competitive canoeing. QMRA results for Cryptosporidium and pathogenic E. coli showed that the probability of infection for most users exceeds the acceptable level for drinking and recreation as outlined in the South African water quality guidelines, and by the World Health Organization (WHO). The results of this study can be used as a baseline to assess the economic and health implications of different management plans, resulting in better-informed, cost-effective, and impactful decision-making. [ABSTRACT FROM AUTHOR]

The mining and mineral processing of Cu-Ni-PGM sulfide ores in South Africa occurs in semi-arid regions. The scarcity of water resources in these regions has become one of the biggest challenges faced by mineral concentrators. As a result, concentrators are forced to find ways through which they can manage and control their water usage. The recycling and re-use of process water in mineral concentration plants has therefore become a common practice. This practice is beneficial in that it reduces reliance on municipal water and harnesses compliance to stringent environmental regulations on freshwater usage. This approach also offers a better response to the Sustainable Development Goals (SDGs) for the mining industry, as water and its preservation form part of the SDGs. This practice could, however, be somewhat concerning to a process operator because recirculated water often has higher concentrations of ions compared to fresh or potable water. This is because an unintended change in the process water quality may affect critical aspects of flotation such as the stability of the froth. This issue has led to the need for both the mining industry and researchers in the field to find the ions in process water that have the greatest impact on froth stability. Thus, the authors of this study investigated the effects of various ions common in the process water of a typical Cu-Ni-PGM ore on froth stability using a 3 L bench scale flotation cell. Solids and water recoveries were used as proxies for froth stability. These were further complemented by bubble size, water recoveries, foam height, and dynamic foam stability from two-phase flotation systems. A two-phase foam study resulted in observations that supported findings from a three-phase study. Generally, single salt solutions containing Ca2+ and Mg2+ ions resulted in higher water recoveries both in the two-phase foam and three-phase froth studies, increases in foam heights and dynamic foam stability, and a decrease in bubble size compared to the solutions that contained Na+. SO42− also resulted in increased foam stability compared to Cl− and NO3−. These results showed that the divalent inorganic electrolytes—Ca2+, Mg2+, and SO42−—were more froth- and foam-stabilizing than the monovalent inorganic electrolytes—Cl−, NO3−, and Na+. This finding was in agreement with previous research. The findings of this study are deemed crucial in the development of a process water management protocol in sulfidic Cu-Ni-PGM ore concentrators. However, more comparative three-phase froth stability tests are needed as subjects of future investigative work to further ascertain specific ion effects on froth stability in sulfide ores. [ABSTRACT FROM AUTHOR]

Benthic macroinvertebrates play a vital role in ecosystem functioning, such as nutrient cycling, primary production, decomposition and material translocation. A study was done to determine the functional feeding groups (FFGs) distribution along two streams of the Olifants River System, South Africa. There were longitudinal differences of the functional groups but were more pronounced in the Dwars River than in the Spekboom River. Collector-gatherers and collector-filterers were the most abundant groups recorded in both rivers. The least abundant groups were the shredders and scrapers in both rivers. The highest abundance of shredders in both rivers was at upstream sites (DS1 and SS1), more scrapers were found at midstream sites (DS3 and SS2), collector-gatherers and collector-filterers increased in the downstream (SS4) of the Spekboom River and predators were nearly constant in relative abundance at all sites. The relative abundance and richness of the FFGs did not conform completely to the river continuum concept (RCC). This could be related to the degradation of the catchment resulting from human activities which affected the quality of the water. [ABSTRACT FROM AUTHOR]

The taxonomy-based response pattern of macroinvertebrates to pollution gradient is well established, with tolerant taxa increasing in impacted conditions, while sensitive taxa increase with decreasing deterioration, typical of rural pollution. This study identified rural indicator and sensitive traits of Ephemeroptera, Plecoptera and Trichoptera (EPT) taxa by examining their trait distribution pattern in relation to rural pollution. Physicochemical parameters and EPT were sampled seasonally from August 2016 to April 2017. Eight sites were selected and categorised into three site groups. Site group 1 served as the least impacted site group and Site group 2 as the moderately influenced, whereas Site group 3 was the most impacted. Seven traits were selected and categorised into 27 trait modalities. The response of EPT traits to physicochemical parameters was analysed using the simultaneous analysis of the information contained in three tables: R (environmental characteristics of samples), L (taxa distribution across samples) and Q (species traits) (RLQ) and confirmed with fourth-corner analysis. Three trait attributes, large (10–20 mm), swimming, shredding, streamlined body shape and large body size (≥ 10–20), were considered tolerant signature traits of semi-urban pollution. These trait attributes were associated with the influenced Site group 3 and indicated a significant positive affinity with at least one physicochemical indicator of increasing semi-urban pollution (NH4-N, NO3-N, NO2-N, PO4-P, EC, turbidity, temperature and pH). Conversely, small body size (< 10 mm), operculate gills, spherical body shape and a preference for sediments were correlated with the least influenced Site group 1 and were considered sensitive traits of semi-urban disturbance. Overall, this study provided critical insights into EPT responses to disturbance, revealing that semi-urban activities influenced EPT traits differently in the Tsitsa River. [ABSTRACT FROM AUTHOR]

The Oceans and Coastal Information Management System (OCIMS) was launched by the South African Government in 2015 to support the development and governance of the South African ocean economy. The OCIMS has established knowledge tools for marine spatial planning, maritime domain awareness, search and rescue, water quality and harmful algal bloom monitoring. Those tools are used daily by stakeholders across government departments, industry, and civil society. Unlike many other operational oceanographic and coastal systems around the world, the OCIMS was designed from its inception using inputs from stakeholders. Continuous engagements between developers and stakeholders have ensured that the system remains fit for purpose. The OCIMS is both locally relevant and globally cognizant. Developments are undertaken to ensure inter-operability with other systems in the world and promote the exchange and discovery of data. The OCIMS project was able to leverage co-funding and the sharing of data and expertise through partnerships across the public and private sectors. These partnerships have been essential to the success of OCIMS and would not have been possible without continued engagements and the sustained funding provided by the South African national government. The development pathway followed to establish the OCIMS could benefit other countries looking to implement their own operational ocean and coastal system knowledge platform. • The Ocean Information Management System supports Blue growth. • The formalization of stakeholder's engagement ensures co-design and relevance. • Adherence to international best practices promotes global visibility. • Co-funding from partners help leverage cost and human capacity. • Secure funding streams from government are essential to ensure sustainability. [ABSTRACT FROM AUTHOR]

Background: Although the Kruger National Park (KNP) is a protected area, it is not exempted from anthropogenically induced impacts, which often compromise river water flow and quality. Measures of river water quality in South Africa have conventionally been based on water chemistry as well as different ecological indicator groups such as fish and macroinvertebrates. Diatoms have been shown to be useful indicators of changes in water quality resulting from pollution and land-use impacts. Objectives: To determine the applicability of diatoms for hind-casting water quality in the KNP and to compare recent diatom community composition with those from the 1980s. Methods: In this study, pH and electrical conductivity (EC) were used to evaluate temporal changes in water quality of three rivers within the KNP. Additionally, we made use of historic diatom samples from three of the five perennial rivers to evaluate if diatom communities reflected changes in water conditions between 1983 and 2015. Results: Diatom community structure was significantly different between 1983 and 2015. Diatom-based index scores (SPI) indicated an improvement in water quality for the Letaba and Olifants rivers. Conclusion: Diatoms were shown to be useful indicators of current water quality and are also useful for determining temporal changes. [ABSTRACT FROM AUTHOR]

Background: The understanding of the impact of land-use on the dynamics of phytoplankton assemblages during varying climate conditions on rivers is limited. Objective: To determine the impact of land-use types and flow on phytoplankton assemblages in the Sabie River. Methods: The relationships between land-use patterns, water quality and phytoplankton assemblages were analysed using canonical correspondence analyses (CCA). Results: Six main land-use types could be distinguished in the eight identified sub-catchments of the river. The CCA results showed that the land-use had a stronger correlation with phytoplankton classes during the higher flow conditions than during low flow conditions. The forestry land-use type had the strongest correlation with nitrate-nitrite concentrations in the Sabie River. Chlorophyll-a concentrations were higher during 2016, and a slightly lower number of genera were observed for the class Chlorophyceae. During 2017 the number of genera of the class Cyanophyceae decreased together with chlorophyll-a and total cell concentrations. Conclusion: Low flow conditions favoured filamentous genera that are common to mesotrophic conditions while higher flow conditions favoured single-celled small genera more common to oligotrophic waters with higher disturbance. This study showed that flow has the potential to enhance the impact of land-use on phytoplankton community dynamics in a river system and thereby may have further impacts on the health and activities of surrounding communities. [ABSTRACT FROM AUTHOR]

Background: Effluents from agricultural activities, metal and peat mining, urban and industrial areas, informal settlements and wastewater treatment plants all influence tributaries feeding the Mooi River. All these land uses ultimately result in a deterioration of water quality in the Mooi River. Objectives: The main objective was to relate the effects of inflowing tributaries to water quality and phytoplankton assemblages in the Mooi River. Method: Physico-chemical environmental variables were compared with water quality objectives and known limits to assess general water quality in the Mooi River over a one-year period. Water quality and phytoplankton in tributaries were compared to that at sites upstream and downstream from their inflows to determine the extent to which the tributaries affected the Mooi River. Multivariate analysis assisted in the interpretation of phytoplankton and physico-chemical data at the different sites. Results: Seven phytoplankton phyla were identified in the Mooi River. Diatoms and green algae were most dense and diverse. Cyanobacteria were responsible for various problems, especially in the Wasgoedspruit where they dominated and reached high densities. Lowest phytoplankton density and diversity were found in the upstream and downstream sections of the river, while maximum density and diversity were found during summer in the middle reaches. The inflow of tributaries, especially the Wasgoedspruit, had a pronounced effect on water quality downstream. Phytoplankton density and diversity were less affected than chemical variables, especially nutrient concentrations. Conclusion: Various land use activities surrounding the Mooi River's tributaries contributed to a deterioration of water quality in the main stream of the Mooi River. [ABSTRACT FROM AUTHOR]

The Eastern Basin chemical acid mine drainage (AMD) treatment plant is one of the world's largest highdensity sludge (HDS) plants, and came into operation in August 2016. The plant is situated near the inoperative Grootvlei Mine in Springs, South Africa, and upstream of the Blesbokspruit Wetland, a former Ramsar Wetland of International Importance, now on the Montreux Record. Since being in operation it has had a major influence on surface water quality along the wetland area. The plant was constructed to mitigate the anticipated decant of AMD water from the abandoned Grootvlei Mine into the Blesbokspruit Wetland. Making use of the BACI (Before-After-Control-Impact) design, this study compares surface water quality of the Blesbokspruit upstream (control site) and downstream (impact site) of the treatment plant for 3-year periods before and after it came into operation. Quarterly water quality data (aluminium, ammonia, chloride, conductivity, dissolved oxygen, fluoride, iron, magnesium, manganese, nitrate, pH, phosphate, sodium and sulphate) from 2013--2019 were used for 5 historical Rand Water monitoring sites along the Blesbokspruit. The current HDS treatment process has negatively influenced conductivity, chloride, magnesium, sodium and sulphate levels downstream. Since the commissioning of the treatment plant, the levels of these parameters have increased significantly. Notably, conductivity and sulphate have reached the management range defined as 'unacceptable' within the framework set out by the Blesbokspruit Forum (which is less stringent than the national guidelines for aquatic ecosystems), with potential impacts on salinization of the Vaal Barrage downstream. However, the significant reduction of iron, ammonia and phosphate concentrations downstream of the plant may be a combined beneficial effect of dilution by increased discharge from the plant and the wetland removing these contaminants. These results highlight the need for further research into possible secondary treatment and desalinisation mechanisms and the potential ecological and downstream water supply implications of increasing salinity within the area. [ABSTRACT FROM AUTHOR]

Wetlands within the catchments of water supply dams have potential to make important contributions to water-related ecosystem services, particularly water quality enhancement. Wetlands are facing growing threats and continuing degradation. There have been limited attempts at evaluating the contribution of South African wetlands, and their rehabilitation, for water quality enhancement and other water-related ecosystem services. A comprehensive and integrated wetland services economic assessment determined the value of the Vyeboom Wetland in the catchment of the Theewaterskloof Dam. The water of this dam is a significant contributor to the City of Cape Town's water supply. The economic valuation model integrates hydrological, catchment status and economic models, with a long-term (80-year monthly) wetland water balance from a calibrated hydrological model as a driver for the nutrient removal aspect. The economic valuation builds on a water quality enhancement model, based on the minimisation of indirect use replacement cost principle, supplemented by a sediment retention and carbon storage assessment. The capability of the economic valuation model is illustrated by assessing the rehabilitation of a 25-ha area in the Vyeboom Wetland, which is currently intact, but is under threat of being severely degraded by the advancing erosion headcut. It was assumed that the current state of the Vyeboom Wetland is almost pristine (only 1 ha degraded), but that a further 24 ha would degrade over a period of 50 years, following spatial changes over time. By balancing the total investment in offsetting the potential loss in benefits, it is evident that an amount of 2.5 million ZAR could be afforded for rehabilitation. If, instead of a logistic degradation profile, the wetland is assumed to already be in a state of accelerated deterioration, the rehabilitation project budget for Vyeboom Wetland will increase once the other intrinsic benefits described are considered in the analysis. [ABSTRACT FROM AUTHOR]