1. Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum
- Author
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Yangyang Wei, Quanwei Lu, Renhai Peng, Baohong Zhang, Zhen Liu, Xinpeng Wen, Shulin Zhang, Yuling Liu, Kunbo Wang, and Fang Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,Chromosomes, Artificial, Bacterial ,Molecular biology ,Quantitative Trait Loci ,lcsh:Medicine ,Cotton ,Biology ,Quantitative trait locus ,DNA construction ,Molecular biology assays and analysis techniques ,01 natural sciences ,Genome ,03 medical and health sciences ,BAC library screening ,Vector cloning ,Gene mapping ,DNA library construction ,Genomic Library Screening ,Genomic library ,Cloning, Molecular ,lcsh:Science ,Genome size ,Gene ,Flowering Plants ,Gene Library ,Cloning ,Genetics ,Bacterial artificial chromosome ,Gossypium ,Multidisciplinary ,Biology and life sciences ,BAC library construction ,lcsh:R ,Organisms ,Eukaryota ,Chromosome Mapping ,Plants ,Genomic Library Construction ,Research and analysis methods ,BAC cloning ,030104 developmental biology ,Molecular biology techniques ,Library screening ,lcsh:Q ,010606 plant biology & botany ,Research Article - Abstract
A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4) was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.
- Published
- 2018