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The objective of this study was to quantify and analyze the level of anthropic changes and its relations with land use, land cover, and soil types in the Diamantino river sub-basin, Mato Grosso State. For this purpose, satellite images of Landsat-5 and Landsat-8 were used, which were processed and classified with the Spring software. The thematic classes were quantified using the ARCgis software. The degree of human disturbance was verified by the Anthropic Transformation Index. The soil types in the basin were quantified, considering a cut-out of the Soils Map from Mato Grosso State, also using ARCgis. Among the classes mapped, pasture presented a significant expansion in the last 20 years, to the detriment of suppression from the natural vegetation in all sub-basins. The soil types Red Latosol and Red-Yellow are prominent in the Diamantino river sub-basin. The calculation of the Anthropic Transformation Index indicated that the level of changes at the Diamantino river sub-basin increased. In all sub-basins, this level was degraded between 1993 and 2013. In this study, the main factor that favored the increase of the Anthropic Transformation Index was the expansion of pasture. [ABSTRACT FROM AUTHOR]