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Three polysaccharides (SnNG, SnFS and SnFG) were purified from the body wall of Stichopus naso. The physicochemical properties, including monosaccharide composition, molecular weight, sulfate content, and optical rotation, were analyzed, confirming that SnFS and SnFG are sulfated polysaccharides commonly found in sea cucumbers. The highly regular structure {3)-L-Fuc2S-(α1,}n of SnFS was determined via a detailed NMR analysis of its oxidative degradation product. By employing β-elimination depolymerization of SnFG, tri-, penta-, octa-, hendeca-, tetradeca-, and heptadeca-saccharides were obtained from the low-molecular-weight product. Their well-defined structures confirmed that SnFG possessed the backbone of {D-GalNAc4S6S-β(1,4)-D-GlcA}, and each GlcA residue was branched with Fuc2S4S. SnFS and SnFG are both structurally the simplest version of natural fucan sulfate and fucosylated glycosaminoglycan, facilitating the application of low-value sea cucumbers S. naso. Bioactivity assays showed that SnFG and its derived oligosaccharides exhibited potent anticoagulation and intrinsic factor Xase (iXase) inhibition. Moreover, a comparative analysis with the series of oligosaccharides solely branched with Fuc3S4S showed that in oligosaccharides with lower degrees of polymerization, such as octasaccharides, Fuc2S4S led to a greater increase in APTT prolongation and iXase inhibition. As the degree of polymerization increases, the influence from the sulfation pattern diminishes, until it is overshadowed by the effects of molecular weight. [ABSTRACT FROM AUTHOR]

Metal–organic framework (MOF) materials have unique structure and fantastic properties for wide-ranging applications. Pilot studies highlighted the toxicity and potential threats of MOF materials to the environment. In this study, we revealed the phytotoxicity of MOF-74(Co) nanoparticles (NPs) and their inhibitory effects on the photosynthesis of pea seedlings (Pisum sativum L.). MOF-74(Co) NPs have limited influences on the germination of pea seeds, but distinct environmental effects of MOF-74(Co) NPs were found in pea seedlings. The root length of pea seedlings, fresh weight and dry weight decreased by 50.0%, 29.2% and 36.4%, respectively, compared with the control group, when the material concentration was greater than 100 mg L−1. The net photosynthetic rate decreased by 48% and the intercellular CO2 concentration increased by 183% upon exposure to MOF-74(Co) NPs. Mechanistically, MOF-74(Co) exposure led to Co uptake in pea seedlings; the increases were 223% for the root, 267% for the stem and 6562% for the leaves, respectively, when the MOF-74(Co) NP concentration was 10 mg L−1. The released Co ions from MOF-74(Co) NPs caused oxidative damage to leaves and induced damage to the acceptor side of photosynthesis system II. Our results indicated that the environmental toxicity of MOF materials was largely regulated by the metal centers. MOF materials with nontoxic metal elements are desirable for future applications. [ABSTRACT FROM AUTHOR]

Drilling pressure relief is a widely recognized and applied key technique to reduce the risk of rock burst occurrence, and its field-applied effects are also obvious. In this paper, the values of micro parameters required for discrete element method (DEM) simulations were first determined through indoor experiments and parameter calibration. Then, drilling pressure relief numerical simulations were conducted with different drilling diameters, drilling angles, and drilling depths. The results show that as the depth and diameter of the borehole increase, the peak strength of the coal sample first rapidly decreases, and then slowly decreases. The 30 mm depth and 14 mm diameter of the borehole are, respectively, the critical thresholds for the transition of the peak strength decrease rate; as the drilling angle increases, the peak strength of the coal sample first rapidly decreases and then increases, and the drilling angle of 25° is the critical threshold for the peak strength transformation. At the same time, the type of coal sample failure also changes from conjugate shear fracture to single slope shear fracture with the increase of three drilling parameters; shear and tensile cracks are the main types of micro fractures in coal samples. The research results are of great significance for improving the pressure relief plan of coal seam drilling and preventing and controlling rock burst disasters. [ABSTRACT FROM AUTHOR]

Zinc indium sulfide (ZnIn2S4), as a significant visible-light-responsive photocatalyst, has become a research hotspot to tackle energy demand and environmental issues owing to its excellent properties of high stability, easy fabrication, and remarkable catalytic activity. However, its drawbacks, including low utilization of solar light and fast photoinduced charge carriers, limit its applications. Promoting the response for near-infrared (NIR) light (~52% solar light) of ZnIn2S4-based photocatalysts is the primary challenge to overcome. In this review, various modulation strategies of ZnIn2S4 have been described, which include hybrid with narrow optical gap materials, bandgap engineering, up-conversion materials, and surface plasmon materials for enhanced NIR photocatalytic performance in the applications of hydrogen evolution, pollutants purification, and CO2 reduction. In addition, the synthesis methods and mechanisms of NIR light-driven ZnIn2S4-based photocatalysts are summarized. Finally, this review presents perspectives for future development of efficient NIR photon conversion of ZnIn2S4-based photocatalysts. [ABSTRACT FROM AUTHOR]

This paper focuses on the effect of fiber orientation on the resistance of seepage-induced erosion in fiber-reinforced sand. To clarify the discrepancy and mechanism of different-oriented fibers improving the resistance of the sand matrix, a series of DEM-Darcy coupling simulations were conducted. The microscopic parameters of fiber-reinforced sand were confirmed by the rigorous calibration procedure. The fibers perpendicular to the seepage direction were found to increase the difficulty of moving fluid through the specimen and significantly reduce the erosion rate of the specimen. These macroscopic behaviors acquired corresponding explanations at the mesoscopic scale, including the evolution of fiber-sand contact orientation, coordination number, average normal contact force, tensile force, and energy dissipation. According to the simulation results, it is found that the highest proportion of tensile force in perpendicular fibers can reach 80%, while the parallel fibers are only 40%, which indicates that the perpendicular fibers have a significant netting effect. The mesoscopic behaviors reasonably revealed the role of the fibers with different orientations on the sand matrix during the seepage. This study is beneficial for further understanding the mechanical behaviors of fiber-reinforced sand under seepage-induced erosion in safety engineering. [ABSTRACT FROM AUTHOR]

With the widespread application of digital impression techniques in prosthetic dentistry, accurate intraoral scan mounting, and virtual articulator parameters setting as per patients' anatomic structures are essential for treatment planning and restoration fabrication, especially for complex rehabilitation cases; meanwhile, marginal fit checking, occlusal adjustment, and porcelain layering of restorations are also crucial procedures in all cases in which the analog procedure to mount maxillary arches on a mechanical articulator is still required. This technique article presents an indirect digital approach that can first achieve virtual intraoral scan mounting and sagittal condylar inclination value setting of an Artex virtual articulator based on bony structures provided by a single cone beam computed tomography scan. It then facilitates the transfer of virtually mounted intraoral scans from the virtual articulator to the matched Artex mechanical articulator by relating a digitally scanned mounting plate of the Artex mechanical articulator to the virtual articulator, printing the intraoral scan and mounting plate scan assembly, and then mounting the printed casts on the mechanical articulator based on the printed mounting plate. This technique eliminates the conventional facebow transfer and protrusive bite registration procedures and offers a straightforward approach for the seamless integration of virtual environments and analog workflows into clinical practice. It aids in the design of restorations that are harmonious with the mandibular movements and reduces chairside adjustment time. [ABSTRACT FROM AUTHOR]

As emerging pollutants, pharmaceutical and personal care products (PPCPs) have received extensive attention due to their high detection frequency (with concentrations ranging from ng/L to μg/L) and potential risk to aqueous environments and human health. Advanced oxidation processes (AOPs) are effective techniques for the removal of PPCPs from water environments. In AOPs, different types of free radicals (HO·, SO4·−, O2·−, etc.) are generated to decompose PPCPs into non-toxic and small-molecule compounds, finally leading to the decomposition of PPCPs. This review systematically summarizes the features of various AOPs and the removal of PPCPs by different free radicals. The operation conditions and comprehensive performance of different types of free radicals are summarized, and the reaction mechanisms are further revealed. This review will provide a quick understanding of AOPs for later researchers. [ABSTRACT FROM AUTHOR]

Skin is the outmost layer of human and sustains most of the external UVB irradiation, which possibly causes the skin photoaging. As a natural antioxidant, marine natural products have been paid more and more attention to their positive effects on photoaging. 6,6′‐bieckol is a phlorotanin isolated from Ecklonia cava, while its antiphotoaging bioactivity and mechanism have not been clear yet. This study proves that 6,6′‐bieckol enhances cells viability and decreases the level of ROS in UVB‐induced human immortalized keratinocytes (HaCaT) cells. It also resulted in significant downregulation of matrix metalloproteinases (MMPs), p‐c‐Fos, phosphorylated JNK, p38, IκB and p65. In addition, molecular docking also showed that 6,6′‐bieckol could bind to MMP‐1, MMP‐3 and MMP‐9. Finally, it was proved that 6,6′‐bieckol acts on MMPs through the MAPK/AP‐1 and NF‐κB pathways to reduce UVB‐induced oxidative stress damage in HaCaT cells. Therefore, 6,6′‐bieckol is a functional food and skin care ingredient with great potential in preventing photoaging. [ABSTRACT FROM AUTHOR]

To investigate the effects of implementing low-load blood flow restriction exercises (LL-BFRE) instead of high-load exercises (HL-RE) in a contrast training program on strength and power performance of high-level young gymnasts. Fifteen high-level pre-pubescent trampoline gymnasts (national level, Tanner Stage II, intermediate experience in strength training) were divided into two groups to complete the same structure of a ten-week contrast strength training program differing only in the configuration of the first resistance exercise of the contrast sequence. The LL-BFRE group (n = 7, four girls, 13.9 ± 0.4 y) performed the first resistance exercise of the contrast with LL-BFRE (20%–30% 1RM, perceived pressure of 7 on a scale from 0 to 10). The HL-RE group (n = 8, four girls, 13.8 ± 0.5 y) trained the first resistance exercise of the contrast sequence with moderate-to-high load (60%–85% 1RM). Before and after the training period, isometric mid-thigh pull (IMTP), squat jump (SJ), counter movement jump (CMJ), and drop-jump (DJ) were performed to evaluate the effect of the intervention on strength and power capacities as primary outcomes. Changes in participants' anthropometric measures, muscle mass, left and right thigh girth, IMTP relative to bodyweight (IMTP-R), eccentric utilization ratio (EUR), and reactive strength index (RSI) were assessed as secondary outcomes. There was no significant interaction (p > 0.05) between group x time in any power and strength outcome, although SJ and EUR showed a trend to significant interaction (p = 0.06 and p = 0.065, respectively). There was an overall effect of time (p < 0.05) in all power and strength variables (CMJ, SJ, EUR, DJ, RSI, IMTP, and IMTP-R). There was a significant interaction in muscle mass (MM) [β = 0.57 kg, 95% CI = (0.15; 0.98), t13 = 2.67, p = 0.019], revealing that participants in LL-BFRE increased their muscle mass (6.6 ± 3.1%) compared to HL-RE (3.6 ± 2.0%). Anthropometric variables did not present any group or interaction effect. However, there was a time effect (p < 0.05). Implementing LL-BFRE in place of HL-RE as a conditioning activity in a contrast training sequence might be equally effective in improving lower-body strength and power in preadolescent trampoline gymnasts. [ABSTRACT FROM AUTHOR]

Customized cast orientations and parameter settings of the virtual articulator according to the patient's condyles are indispensable parts of today's digital workflows in prosthodontics. This article describes a digital technique to align the intraoral scans to a virtual articulator by using a facial scanner to locate the patient's cutaneous landmarks of the arbitrary hinge axis and the reference plane, and to customize the sagittal condylar inclination of the virtual articulator through a digital protrusive interocclusal record and a dental computer‐aided design software program. It enables individual cast orientations and virtual articulator parameter settings without conventional facebow transferring and bite registration procedures and can be easily integrated with most virtual articulator systems on the market to allow clinicians and technicians to work in a complete digital workflow and facilitate customized treatment planning and dental prosthesis fabrication. [ABSTRACT FROM AUTHOR]

• Discrete element method study on sand-tyre chips (STCh) with different tyre chip orientations is presented. • Rigorous calibration procedures for obtaining the microscopic parameters of STCh mixtures are presented. • The discrepancy in the reinforcement mechanism of different oriented tyre chips from a micromechanical viewpoint was studied. • It provides a theoretical reference for the design of scrap tyre chips in geotechnological application. Sand-tyre chips (STCh) mixtures are emerging civil engineering materials that have been widely studied and applied. In the sand-tyre chips system, the mechanical behavior of sand matrix is improved attributed to the excellent property of tyres. However, the effect of tyre chip orientation for STCh mixtures is not fully appreciated, especially from the microscopic scale. In this paper, the microscopic behaviors of STCh mixtures with different orientation tyre chips were investigated employing the Discrete Element Method (DEM). The tyre chips model was constructed with sufficient consideration and the microscopic parameters of STCh model were confirmed by the calibration procedure. Simulation results illustrate that horizontal tyre chips more effectively enhance the mechanical properties of mixtures. The visualization of the specimen shows that the orientation of tyre chips can influence the evolution of system contact network, formation of shear band, and bulging deformation of specimen. The microscopic mechanism including the composition of strong contacts, coordination number, average normal contact force, sliding and rolling, energy storage and dissipation, and the tensile force in tyre chips were studied to evaluate the discrepancies of different orientation tyre chips. The mechanism of the reinforcement for tyre chips can receive further understanding from this study. [ABSTRACT FROM AUTHOR]

Periphyton has important ecological functions. It can even exist in environments with strong ultraviolet radiation. However, knowledge of periphyton under ultraviolet is limited, which limits the understanding and application of periphyton in environments with high ultraviolet radiation. In this study, immature periphyton (IMP) and mature periphyton (MP) under ultraviolet B (UVB) irradiation were investigated and compared in terms of physiological characteristics and bacterial community. Analysis of the physiological characteristics showed that the response patterns of IMP and MP to UVB were similar. IMP and MP could adapt to UVB of 1 W/m2 well. However, high-intensity UVB (2 and 3 W/m2) reduced the periphyton biomass, inhibited photosynthesis and antioxidant enzyme activity and caused severe lipid peroxidation in both IMP and MP. Integrated Biological Response (IBR) analysis and toxicological model fitting showed that the ED 50 values of UVB for IMP and MP were 1.25 and 1.50 W/m2, respectively. 16 S rRNA gene analysis showed that in both IMP and MP, bacterial community composition, assembly and function were affected by UVB. In addition, the response of the bacterial community in IMP to UVB was stronger than that in MP. The diversity of the IMP community was inhibited by UVB, but that of the MP community was not. Proteobacteria and Deinococcus-Thermus are key microorganisms responsible for tolerance to UVB stress. Neutral community model fitting showed that both UVB and the development process caused the determinism of bacterial succession. However, UVB may weaken the deterministic process caused by development. Functional prediction showed that many metabolic functions of periphyton were inhibited by UVB in IMP and MP. However, UVB caused different changes (enhancement or inhibition) of some ecological functions in them. This study provides valuable information for understanding periphyton in environments with UVB radiation, which may be used to improve the application of periphyton in these environments. [Display omitted] • IMP and MP under UVB were studied by physiological indicators and bacterial community. • The physiological responses of IMP and MP to UVB were similar. • UVB affected bacterial community composition, assembly and function. • The response of the bacterial community in IMP to UVB is more sensitive than that in MP. [ABSTRACT FROM AUTHOR]

Excessive exposure to Ultraviolet (UV) rays can cause premature skin aging. Ishigoside (IGS) is a new glyceroglycolipid compound isolated from brown algal Ishige okamurae , However, whether it can protect the skin from (Ultraviolet-B) UVB damage has not been illuminated. The in vitro anti -photoaging effect of IGS was conducted in UVB-induced HaCaT. The HaCaT cells were divided into the following five groups: (1) cells didn't suffer from UVB irradiation or IGS treatment. (2–5) Cells were treated with various concentrations of IGS (0, 10, 50, and 100 μ M) and irradiated by 40 mJ/cm2 UVB. The Matrix metalloproteinase (MMP) of photoaging process was determined by ELISA kits and the latent interaction between IGS and MMP was further performed by molecular docking. The crucial signaling pathway proteins involved in the collagen synthesis and degradation were subsequently evaluated by Western blotting, immunofluorescence and EMSA. IGS effectively suppresses the high expressions and secretions of matrix metalloproteinases (MMPs) and photo-inflammation by blocking MAPKs, AP-1 and NF-κB. Meanwhile, increasing antioxidant enzyme expression. Molecular docking results suggest that inhibition of IGS on MMPs may be attributed to its hydrogen supply and hydrophobic capacity. In addition, IGS enhanced procollagen production by upregulating the TGF-β/Smad pathways. IGS exhibited anti -photoaging activity in UVB-damage HaCaT. These effects might be a contribution by its suppression of MMPs expression via MAPKs, AP-1 and NF-κB pathway and have anti-oxidative and anti-inflammatory effects. Therefore, IGS has the great potential to become skin-care products or functional foods for preventing skin photoaging. [Display omitted] • Ishigoside is the functional ingredient of edible brown algae Ishige Okamurae. • Photoprotectant ishigoside has the potential to develop into healthy food. • Ishigoside reduced matrix metalloproteinases expression and alleviate inflammation via affecting the MAPK/AP-1 and NF-κB pathways. • Ishigoside can inhibitory binding with MMPs by molecular docking analysis. [ABSTRACT FROM AUTHOR]

Schizophrenia is associated with reduced numbers of spines and dendrites from layer III of the dorsolateral prefrontal cortex (dlPFC), the layer that houses the recurrent excitatory microcircuits that subserve working memory and abstract thought. Why are these synapses so vulnerable, while synapses in deeper or more superficial layers are little affected? This review describes the special molecular properties that govern layer III neurotransmission and neuromodulation in the primate dlPFC and how they may render these circuits particularly vulnerable to genetic and environmental insults. These properties include a reliance on NMDA receptor rather than AMPA receptor neurotransmission; cAMP (cyclic adenosine monophosphate) magnification of calcium signaling near the glutamatergic synapse of dendritic spines; and potassium channels opened by cAMP/PKA (protein kinase A) signaling that dynamically alter network strength, with built-in mechanisms to take dlPFC "offline" during stress. A variety of genetic and/or environmental insults can lead to the same phenotype of weakened layer III connectivity, in which mechanisms that normally strengthen connectivity are impaired and those that normally weaken connectivity are intensified. Inflammatory mechanisms, such as increased kynurenic acid and glutamate carboxypeptidase II expression, are especially detrimental to layer III dlPFC neurotransmission and modulation, mimicking genetic insults. The combination of genetic and inflammatory insults may cross the threshold into pathology. [ABSTRACT FROM AUTHOR]

Objective Materials and Methods Results Conclusion To assess a newly developed intraoral scan protocol in enhancing the accuracy of complete‐arch implant impressions.Four impression approaches were applied to the same maxillary edentulous model with 6 implants: (1) intraoral scan (IOS), (2) intraoral scan with scan aid (IOS‐SA), (3) calibrated intraoral scan protocol (CISP), and (4) conventional splinted open‐tray impression (CONV). Each approach was repeated 10 times, and a direct scan of the model with a desktop scanner was used as a reference model. The alignment of scans and the reference model was conducted by two methods: (a) aligning all scan bodies to evaluate the overall fit, and (b) aligning the first and second scan bodies to simulate the Sheffield fit test for passive fitting of multiple implant‐supported prostheses. Linear deviations from the reference model (trueness) and within each group (precision) were analyzed using Python scripts.When aligned by all scan bodies, the CISP group exhibited comparable mean trueness (38.33 μm) and precision (45.97 μm) to the CONV group (44.30 and 47.92 μm respectively), both of which significantly outperformed the IOS group (86.82 and 83.17 μm, respectively). Furthermore, in the virtual Sheffield fit test, the CISP group achieved the highest levels of mean trueness at the end span (121.7 μm), making a linear deviation reduction of 36.7%, 60%, and 41.4% when compared to the CONV, the IOS, and the IOS‐SA groups, respectively. Moreover, the CISP group (104.3 μm) displayed a remarkable 65, 182, and 86 μm advantage in precision over the CONV, IOS, and IOS‐SA groups, respectively.CISP demonstrated comparable accuracy to the gold standard, the conventional splinted open‐tray impression. Furthermore, it excelled in the virtual passive fitting test. [ABSTRACT FROM AUTHOR]

The unfrozen water content (ω u) is an important parameter affecting the hydrothermal-mechanical characteristics of soil and is of important significance with regard to engineering construction and environmental effects in cold regions. In this study, a widely-distributed special humus soil called turfy soil, with poor engineering geological properties, from seasonally frozen regions of northeastern China was investigated. Soil fundamental properties were determined, and ω u under various temperatures during a freeze–thaw process was measured by the nuclear magnetic resonance (NMR) method. Based on NMR theory and transverse relaxation time (T 2) distribution curves, two thresholds were determined to divide the types of pore water in soil. Soil freezing characteristic curves (SFCC) of the total and pore water were drawn, and changes in the internal microstructure and pore characteristics of the soil during the freeze–thaw process were analyzed. The results showed that a drastic phase change within the soil occurs during −2 °C to −4 °C, and the freezing process can be divided into three stages. The formation of small pores and the connection of large pores make the soil looser after freeze–thaw. The division of pore water and variation in ω u show that freezing starts from large pores, while thawing starts from small pores. The capillary water content significantly decreases after freeze–thaw, and the bulk water content tends to increase. At freezing Stage I, ω u is closely related to the initial properties of the soil, whereas ω u at −3 °C almost determines the value of ω u during subsequent freeze–thaw. Finally, a ω u -power function for turfy soil was proposed, and good fitting results were obtained for both freezing and thawing soil. This work can serve as the basis of studies on soil with high organic matter content as well as soil unfrozen water content during freeze–thaw cycles in cold regions. • Two T 2 cutoffs were determined to identify the pore water types of turfy soil. • Capillary water within turfy soil decreased significantly after freeze–thaw. • Pores formed and larger pores connected due to frost heaving after freeze–thaw. • ω u after drastic phase change affected ω u during the freeze–thaw process. • ω u parameterization considering of turfy soil properties showed good effects. [ABSTRACT FROM AUTHOR]

Accurately mounting dental casts on an articulator is an essential step in prosthetic treatments. In digital dentistry, virtual articulator mounting procedures rely on virtual facebow records. However, virtual facebow records usually require devices like face scanners or jaw motion tracking systems that are not commonly available in most dental practices. The present technique report describes a straightforward intraoral scanner-based virtual facebow transfer approach. In this technique, a reference facebow joint support was first scanned and aligned with a virtual articulator. Then, a patient's facebow joint support and bite fork assembly were scanned chairside with an intraoral scanner and aligned with the virtual articulator by matching common features with the reference facebow joint support. After aligning the patient's intraoral scans with the patient's bite fork scan that was already superimposed on the virtual articulator, a virtual mounting process was achieved. Once the corresponding reference facebow joint supports have been generated, this technique can be easily implemented with most facebow systems and be seamlessly integrated into daily clinical practice as only an intraoral scanner and a conventional facebow were required., (© 2024 The Author(s). Journal of Prosthodontics published by Wiley Periodicals LLC on behalf of American College of Prosthodontists.)

Cognitive deficits from dorsolateral prefrontal cortex (dlPFC) dysfunction are common in neuroinflammatory disorders, including long-COVID, schizophrenia and Alzheimer's disease, and have been correlated with kynurenine inflammatory signaling. Kynurenine is further metabolized to kynurenic acid (KYNA) in brain, where it blocks NMDA and α7-nicotinic receptors (nic-α7Rs). These receptors are essential for neurotransmission in dlPFC, suggesting that KYNA may cause higher cognitive deficits in these disorders. The current study found that KYNA and its synthetic enzyme, KAT II, have greatly expanded expression in primate dlPFC in both glia and neurons. Local application of KYNA onto dlPFC neurons markedly reduced the delay-related firing needed for working memory via actions at NMDA and nic-α7Rs, while inhibition of KAT II enhanced neuronal firing in aged macaques. Systemic administration of agents that reduce KYNA production similarly improved cognitive performance in aged monkeys, suggesting a therapeutic avenue for the treatment of cognitive deficits in neuroinflammatory disorders.

Precise alignment between digital arch scans and cone beam computed tomography (CBCT) scans is a crucial step in computer-aided implant planning and placement. However, clinicians frequently encounter challenges during this process when imaging patients with existing metal restorations or orthodontic devices, as these can introduce metallic artifacts on CBCT scans that lead to alignment deviations. The presented technique describes a straightforward approach using a radiopaque occlusal registration material as a radiographic marker to facilitate the alignment between digital arch scans and CBCT scans with metallic artifacts. This technique simplifies the clinical workflow by eliminating the need for additional radiographic templates or specialized devices, offering a cost-effective option for clinicians., (Copyright © 2024 Editorial Council for The Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)

Importance: The risk of mental disorders is consistently associated with variants in CACNA1C (L-type calcium channel Cav1.2) but it is not known why these channels are critical to cognition, and whether they affect the layer III pyramidal cells in the dorsolateral prefrontal cortex that are especially vulnerable in cognitive disorders., Objective: To examine the molecular mechanisms expressed in layer III pyramidal cells in primate dorsolateral prefrontal cortices., Design, Setting, and Participants: The design included transcriptomic analyses from human and macaque dorsolateral prefrontal cortex, and connectivity, protein expression, physiology, and cognitive behavior in macaques. The research was performed in academic laboratories at Yale, Harvard, Princeton, and the University of Pittsburgh. As dorsolateral prefrontal cortex only exists in primates, the work evaluated humans and macaques., Main Outcomes and Measures: Outcome measures included transcriptomic signatures of human and macaque pyramidal cells, protein expression and interactions in layer III macaque pyramidal cells using light and electron microscopy, changes in neuronal firing during spatial working memory, and working memory performance following pharmacological treatments., Results: Layer III pyramidal cells in dorsolateral prefrontal cortex coexpress a constellation of calcium-related proteins, delineated by CALB1 (calbindin), and high levels of CACNA1C (Cav1.2), GRIN2B (NMDA receptor GluN2B), and KCNN3 (SK3 potassium channel), concentrated in dendritic spines near the calcium-storing smooth endoplasmic reticulum. L-type calcium channels influenced neuronal firing needed for working memory, where either blockade or increased drive by β1-adrenoceptors, reduced neuronal firing by a mean (SD) 37.3% (5.5%) or 40% (6.3%), respectively, the latter via SK potassium channel opening. An L-type calcium channel blocker or β1-adrenoceptor antagonist protected working memory from stress., Conclusions and Relevance: The layer III pyramidal cells in the dorsolateral prefrontal cortex especially vulnerable in cognitive disorders differentially express calbindin and a constellation of calcium-related proteins including L-type calcium channels Cav1.2 (CACNA1C), GluN2B-NMDA receptors (GRIN2B), and SK3 potassium channels (KCNN3), which influence memory-related neuronal firing. The finding that either inadequate or excessive L-type calcium channel activation reduced neuronal firing explains why either loss- or gain-of-function variants in CACNA1C were associated with increased risk of cognitive disorders. The selective expression of calbindin in these pyramidal cells highlights the importance of regulatory mechanisms in neurons with high calcium signaling, consistent with Alzheimer tau pathology emerging when calbindin is lost with age and/or inflammation.

With the development of digital dental technologies, a complete digital workflow without using physical casts has become possible. However, for certain clinical and dental laboratory procedures, especially in complex rehabilitation treatments, physically mounted casts in an ideal location in a mechanical articulator are still necessary for treatment planning and restoration fabrication. This technique report describes a digital approach to fabricating a custom transfer plate to cross mount intraoral scan casts from a virtual articulator to the corresponding mechanical articulator. This technique eliminates the need for conventional physical facebow transfer processes and offers a straightforward approach to integrating virtual procedures with analog workflows., (Copyright © 2024 Editorial Council for The Journal of Prosthetic Dentistry. All rights reserved.)

Objectives: This clinical study aimed to assess the trueness of three intraoral scanners for the recor-ding of the maximal intercuspal position (MIP) to provide a reference for clinical practice., Methods: Ten participants with good occlusal relationship and healthy temporomandibular joint were recruited. For the control group, facebow transferring procedures were performed, and bite registrations at the MIP were used to transfer maxillary and mandibular casts to a mechanical articulator, which were then scanned with a laboratory scanner to obtain digital cast data. For the experimental groups, three intraoral scanners (Trios 3, Carestream 3600, and Aoralscan 3) were used to obtain digital casts of the participants at the MIP following the scanning workflows endorsed by the corresponding manufacturers. Subsequently, measurement points were marked on the control group's digital casts at the central incisors, canines, and first molars, and corresponding distances between these points on the maxillary and mandibular casts were measured to calculate the sum of measured distances (D A ). Distances between measurement points in the incisor (D I ), canine (D C ), and first molar (D M ) regions were also calculated. The control group's maxillary and mandibular digital casts with the added measurement points were aligned with the experimental group's casts, and D A , D I , D C , and D M values of the aligned control casts were determined. Statistical analysis was performed on D A , D I , D C , and D M obtained from both the control and experimental groups to evaluate the trueness of the three intraoral scanners for the recording of MIP., Results: In the control group, D A , D I , D C , and D M values were (39.58±6.40), (13.64±3.58), (14.91±2.85), and (11.03±1.56) mm. The Trios 3 group had values of (38.99±6.60), (13.42±3.66), (14.55±2.87), and (11.03±1.69) mm. The Carestream 3600 group showed values of (38.57±6.36), (13.56±3.68), (14.45±2.85), and (10.55±1.41) mm, while the Aoralscan 3 group had values of (38.16±5.69), (13.03±3.54), (14.23±2.59), and (10.90±1.54) mm. Analysis of variance revealed no statistically significant differences between the experimental and control groups for overall deviation D A ( P =0.96), as well as local deviations D I ( P =0.98), D C ( P =0.96), and D M ( P =0.89)., Conclusions: With standardized scanning protocols, the three intraoral scanners demonstrated comparable trueness to traditional methods in recording MIP, fulfilling clinical requirements.

A digital approach to fabricating a custom holder to attach a mandibular sensor of an optical jaw motion tracking system is described. Typically, jaw motion tracking systems come with standard holders. However, additional chairside time is still required to adapt the holder's arm to the individual arch and securely attach the holder to the mandibular teeth. Moreover, the placement of the standard holder is problematic in patients with a deep vertical overlap or with short clinical crowns. This technique offers a digital approach to designing and fabricating a custom holder in situations where standard holders cannot be efficiently attached. The custom holder is designed to accommodate the available space without interfering with the occlusion, thereby minimizing the time needed to attach the holder and optimizing the workflow for clinical jaw motion tracking., (Copyright © 2024 Editorial Council for The Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)

Objectives: This clinical study aimed to evaluate the accuracy of a fully digital technique for measuring sagittal condylar inclination (SCI), as well as validating whether differences existed between the left and right SCI values of the same participant, to provide a reference for clinical practice., Methods: Ten participants with good occlusal relationship and normal temporomandibular joint were recruited. Three methods were used to measure the SCI values of the participants, namely, A (mechanical facebow transferring and mechanical articulator-based measuring method with physical protrusive interocclusal registration), B (face scan-based virtual facebow and virtual articulator-based measuring method with digital protrusive interocclusal registration), and C (jaw motion tracking system-based measuring method). With the group subjected to methods A and C as the control, the SCI values obtained by the three methods were statistically analyzed. The left and right SCI values of the same participant were also compared., Results: The left and right SCI values measured by method A were 41.70°±7.09° and 42.80°±8.62°, those by method B were 35.09°±12.49° and 37.63°±12.10°, and those by method C were 39.43°±8.72° and 38.45°±6.91°. No significant difference existed among the SCI values measured by the three methods ( P >0.05). Meanwhile, no statistical difference existed between the SCI values on the left and right sides of the same participant ( P >0.05)., Conclusions: The accuracy of the virtual facebow and digital protrusive occlusal registration based SCI measuring method was the same as that of mechanical facebow based and jaw motion tracking system-based methods. The SCI values on the left and right sides of the same participant were similar. Clinically, an appropriate SCI measurement and setting strategy can be selected based on the actual situations.

A digital approach is described for fabricating a splinted framework and custom open tray for the impression of multiple implants based on a virtually created implant cast with the digital prediction of the future impression coping positions. In this technique, impression copings to be used in the definitive impression-making process were digitally scanned and aligned to a preliminary cast using installed protective caps of screw-retained abutments as references, which resulted in a virtual cast that offers information both about the impression coping positions and their surrounding tissues and could allow a splinted framework and custom open tray to be digitally designed and fabricated. Creation of a virtual cast through digital technologies eliminates the traditional manual splinted framework and custom tray fabrication procedures. Additionally, only one set of impression copings and analogs is needed in this technique, thereby reducing treatment time and cost. It also offers a splinted framework and custom open tray with consistent quality., (© 2023 by the American College of Prosthodontists.)

Background: The most prevalent cancer and the second-leading cause of cancer-related mortality in women is breast cancer. Growing interest has been shown in recent years in learning more about the processes behind the development of breast cancer. It has been shown that persistent inflammation may play a significant role in the advancement of breast cancer. However, a comprehensive and objective analysis on the state of inflammation in breast cancer research is still lacking. This study was aim to undertake a bibliometric analysis of breast cancer research associated with inflammation between 2013 and 2022 in order to identify the trends, dynamics, and scientific outputs in the field., Methods: From 2013 to 2022, original and review publications on breast cancer and inflammation-associated research were retrieved from the Web of Science Core Collection (WOSCC) database. To examine the position of yearly publications, journals, nations, institutions, and authors, we employed two bibliometric tools (CiteSpace and VOSviewer). After that, by examining keyword visualization and keyword bursts, we determined the hot research fields related to inflammation in breast cancer., Results: we discovered 6902 publications regarding inflammation in breast cancer by using our retrieval approach. In terms of the number of publications, The United States ranked first in the global study, followed by China and Italy. In terms of institutions, the University of Texas System, UT MD Anderson Cancer Center, and University of California System are in the top 3 for the quantity of publications published. The most popular journal for this field research is "CANCERS." Ueno NT, Woodward WA, Cristofanilli M, and others have made significant contributions to the understanding of inflammation in breast cancer. In the end, we conducted a biclustering analysis on keywords and discovered three clusters that represent research hotspots., Conclusion: According to the global trend, the research output of inflammation in breast cancer is increasing. The information provided in this article, including the cooperation network information of authors, nations, journals, and institutions, may help researchers to better understand hotspots and developing patterns in this discipline. At present, the focus of study gradually shifts from "phenotype study" to "therapeutic research". It is recommended to pay attention to the latest hot spots, such as targeted therapy, antimicrobial activity and nanoparticle., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Meng, Xu, Yang, Chen, Wang, Hu, Zheng and Guo.)

Introduction: Gamma-aminobutyric acid (GABA), one of the main active components in Moringa oleifera leaves, can be widely used to treat multiple diseases including inflammation., Methods: In this study, the anti-inflammatory activity and the underlying anti-inflammatory mechanism of the GABA-enriched Moringa oleifera leaves fermentation broth (MLFB) were investigated on lipopolysaccharide (LPS)-induced RAW 264.7 cells model. The key active components changes like total flavonoids, total polyphenols and organic acid in the fermentation broth after fermentation was also analyzed., Results: ELISA, RT-qPCR and Western blot results indicated that MLFB could dose-dependently inhibit the secretions and intracellular expression levels of pro-inflammatory cytokines like 1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α). Furthermore, MLFB also suppressed the expressions of prostaglandin E 2 (PGE 2 ) and inducible nitric oxide synthase (iNOS). Moreover, the mRNA expressions of the key molecules like Toll-like receptor 4 (TLR-4) and nuclear factor (NF)-κB in the NF-κB signaling pathway were also restrained by MLFB in a dose-dependent manner. Besides, the key active components analysis result showed that the GABA, total polyphenols, and most organic acids like pyruvic acid, lactic acid as well as acetic acid were increased obviously after fermentation. The total flavonoids content in MLFB was still remained to be 32 mg/L though a downtrend was presented after fermentation., Discussion: Our results indicated that the MLFB could effectively alleviate LPS-induced inflammatory response by inhibiting the secretions of pro-inflammatory cytokines and its underlying mechanism might be associated with the inhibition of TLR-4/NF-κB inflammatory signaling pathway activation. The anti-inflammatory activity of MLFB might related to the relative high contents of GABA as well as other active constituents such as flavonoids, phenolics and organic acids in MLFB. Our study provides the theoretical basis for applying GABA-enriched Moringa oleifera leaves as a functional food ingredient in the precaution and treatment of chronic inflammatory diseases., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zheng, Lu, Yang, Zou, Zeng, Xiong, Cao and Zhou.)

This paper focuses on the effect of fiber orientation on the resistance of seepage-induced erosion in fiber-reinforced sand. To clarify the discrepancy and mechanism of different-oriented fibers improving the resistance of the sand matrix, a series of DEM-Darcy coupling simulations were conducted. The microscopic parameters of fiber-reinforced sand were confirmed by the rigorous calibration procedure. The fibers perpendicular to the seepage direction were found to increase the difficulty of moving fluid through the specimen and significantly reduce the erosion rate of the specimen. These macroscopic behaviors acquired corresponding explanations at the mesoscopic scale, including the evolution of fiber-sand contact orientation, coordination number, average normal contact force, tensile force, and energy dissipation. According to the simulation results, it is found that the highest proportion of tensile force in perpendicular fibers can reach 80%, while the parallel fibers are only 40%, which indicates that the perpendicular fibers have a significant netting effect. The mesoscopic behaviors reasonably revealed the role of the fibers with different orientations on the sand matrix during the seepage. This study is beneficial for further understanding the mechanical behaviors of fiber-reinforced sand under seepage-induced erosion in safety engineering.

Objective: Metastatic colon cancer (mCC) poses a great threat to the survival of patients suffering from it. In the past decade, many clinical trials have been carried out to improve the prognosis of patients with mCC. Numerous treatments have emerged, and satisfactory efficacy has been demonstrated in randomized phase III trials in highly selective patients with mCC. Our present study aims to investigate whether these therapeutic advances can be reflected to the broader mCC patients who performed cytoreductive colectomy. Method: General and prognostic data for patients diagnosed with mCC who underwent cytoreductive colectomy between 2004-2018 were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. Survival was analyzed using the Kaplan-Meier method and Cox proportional hazards model. The hazard ratio (HR) and its 95% confidence interval (CI) were used to evaluate the influence of risk factors on prognosis. Results: A total of 26,301 patients diagnosed with mCC treated with cytoreductive colectomy were included in this study. The median overall survival was 19 months (range, 17-23). The good prognosis was associated with patients diagnosed at the most recent year, younger age, non-black race, female, married, without previous history of malignancy, no second malignancy onset, descending/sigmoid/splenic flexure colon tumor, normal CEA levels at diagnosis, low primary tumor burden, T1/T2 stage, N0 stage, single organ metastasis, underwent surgical resection of synchronous distant metastatic lymph nodes or organs, a high number of lymph-node examinations, low positive lymph-node ratio and received adjuvant chemotherapy. The proportion of patients surviving for ≥24 months increased from 37% in 2004 to 44.2% in 2016 ( p < 0.001), especially in ≤49 years patients [46.8% in 2004 to 57.8% in 2016 ( p < 0.001)]. The percentage of patients who died within 3 months decreased between 2004 and 2018 (from 19.6% to 15.7%; p < 0.001). Conclusion: Over a span of 15 years, the long-term survival has improved in real-world mCC patients who were treated with cytoreductive colectomy, especially among younger patients. However, the median overall survival remains not substantial., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Meng, Yang and Chen.)

Nanomaterials with intrinsic enzyme-mimicking characteristics, refered to as nanozymes, have become a hot research topic owing to their unique advantages of comparative low cost, high stability and large-scale preparation. Among them, Single-atom nanozymes (SAzymes), as novel nanozymes with abundant atomically dispersed active sites, have caused specific attention in the development of nanozymes for their remarkable catalytic activities, maximum atomic utilization and excellent selectivity, the homogeneous catalytic sites and clear catalytic mechanisms. Herein, a novel single-atom nanozyme based on Fe(III)-doped polydiaminopyridine nanofusiforms (Fe-PDAP SAzyme) was successfully proposed via facile oxidation polymerization strategy. With well-defined coordination structure and abundant Fe-Nx active sites similar to natural metalloproteases, the Fe-PDAP SAzyme exhibits superior peroxidase-like activity by efficiently decomposing H₂O₂ for hydroxyl radical (.OH) species formation. Based on their superior peroxidase-like activity, colorimetric biosensing of H₂O₂ and glucose in vitro was performed by using a typical 3,3,5,5-tetramethylbenzidine through a multienzyme biocatalytic cascade platform, exhibiting the superior specificity and sensitivity. This work not only provides a novel promising SAzyme-based biosensor but also paves an avenue for evaluating enzyme activity and broadens the application of other nanozyme-based biosensors in the fields of biomedical diagnosis.

Laurencia undulata , as one of the most biologically active species in the genus Laurencia , is an edible folk herb red algae. Among them, d-isofloridoside (DIF, 940.68 Da) is isolated from Laurencia undulata , which has antioxidant and matrix metalloproteinases (MMP) inhibitory activities. However, its mechanism of action on tumor angiogenesis has not yet been reported. In this study, we have studied the mechanism of DIF on tumor metastasis and angiogenesis in HT1080 cell and human vascular endothelial cell (HUVEC). The results show that DIF can reduce the activity of MMP-2/9, and can inhibit the expression of hypoxia-inducible factor-1α (HIF-1α) by regulating the downstream PI3K/AKT and mitogen-activated protein kinases (MAPK) pathways, thereby down-regulating the production of vascular endothelial growth factor (VEGF) in CoCl 2 -induced HT1080 cell. In addition, DIF can inhibit the activation of VEGF receptor (VEGFR-2), regulate downstream PI3K/AKT, MAPK, nuclear factor-kappa B (NF-κB) signal pathways, activate apoptosis, and thus down-regulate the production of platelet-derived growth factor (PDGF) in VEGF-induced HUVEC. In conclusion, our research shows that DIF has the potential to develop into a tumor-preventing functional food and tumor angiogenesis inhibitor, and it can provide theoretical guidance for the high-value comprehensive utilization of edible red algae Laurencia undulata .

Gelidium crinale , the red algae belonging to Geliaceae Gelidium , is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe 2+ -chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Pei, Yang, Xiao, Zhou, Hong and Qian.)