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Introduction: Biofilm-associated infections persist as a therapeutic challenge in contemporary medicine. The efficacy of antibiotic therapies is ineffective in numerous instances, necessitating a heightened focus on exploring novel antibiofilm medical strategies. Among these, iminosugars emerge as a distinctive class of compounds displaying promising biofilm inhibition properties. Methods: This study employs an in vivo wound infection mouse model to evaluate the effectiveness of PDIA in treating biofilm-associated skin wound infections caused by Staphylococcus aureus and Pseudomonas aeruginosa. Dermic wounds in mice were infected with biofilm-forming strains, specifically S. aureus 48 and P. aeruginosa 5, which were isolated from patients with diabetic foot, and are well-known for their strong biofilm formation. The subsequent analysis included clinical, microbiological, and histopathological parameters. Furthermore, an exploration into the susceptibility of the infectious strains to hydrogen peroxide was conducted, acknowledging its potential presence during induced inflammation in mouse dermal wounds within an in vivo model. Results: The findings revealed the efficacy of PDIA iminosugar against the S. aureus strain, evidenced by a reduction in bacterial numbers within the wound and the inflammatory focus. Discussion: This study suggests that PDIA iminosugar emerges as an active and potentially effective antibiofilm agent, positioning it as a viable treatment option for staphylococcal infections. [ABSTRACT FROM AUTHOR]

Due to their lack of driving controllability, overweight vehicles are a big threat to road safety. The proposed method for a moving passenger car load estimation is capable of detecting an overweight vehicle, and thus it finds its application in road safety improvement. The weight of a car's load entering or leaving a considered zone, e.g., industrial facility, a state, etc., is also of concern in many applications, e.g., surveillance. Dedicated vehicle weight-in-motion measurement systems generally use expensive load sensors that also require deep intervention in the road while being installed and also are calibrated only for heavy trucks. In this paper, a vehicle magnetic profile (VMP) is used for defining a load parameter proportional to the passenger vehicle load. The usefulness of the proposed load parameter is experimentally demonstrated in field tests. The sensitivity of the VMP to the load change results from the fact that the higher load decreases the vehicle clearance value which in turn increases the VMP. It is also shown that a slim inductive-loop sensors allows the building of a load estimation system, with a maximum error around 30 kg, which allows approximate determination of the number of passengers in the car. The presented proof of concept extends the functionality of inductive loops, already installed in the road, for acquiring other traffic parameters, e.g., moving vehicle axle-to-axle distance measurement, to road safety and surveillance related applications. [ABSTRACT FROM AUTHOR]