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Microalgal lipids are promising and sustainable sources for the production of third-generation biofuels, foods, and medicines. A high lipid yield during the extraction process in microalgae could be influenced by the suitable pretreatment and lipid extraction methods. The extraction method itself could be attributed to the economic and environmental impacts on the industry. This review summarizes the pretreatment methods including mechanical and non-mechanical techniques for cell lysis strategy before lipid extraction in microalgae biomass. The multiple strategies to achieve high lipid yields via cell disruption techniques are discussed. These strategies include mechanical (shear forces, pulse electric forces, waves, and temperature shock) and non-mechanical (chemicals, osmotic pressure, and biological) methods. At present, two techniques of the pretreatment method can be combined to increase lipid extraction from microalgae. Therefore, the extraction strategy for a large-scale application could be further strengthened to optimize lipid recovery by microalgae. [ABSTRACT FROM AUTHOR]

This document, titled "Agronomy 12," is a research article that focuses on sustainable waste management in agriculture and livestock industries. It emphasizes the importance of composting as a natural process that converts organic waste into nutrient-rich compost, which can enhance soil health and fertility. The article discusses the benefits of composting, such as reducing the need for chemical fertilizers and mitigating climate change by sequestering carbon in the soil. It also acknowledges the challenges and disadvantages of composting, including the need for proper management to prevent contamination and the presence of potentially harmful elements. Additionally, the article explores alternative methods of waste utilization, such as using waste materials for mushroom cultivation. Overall, this scholarly article provides valuable insights into sustainable agriculture and food systems and can be a helpful resource for library patrons conducting research in this field. [Extracted from the article]

The use of chitosan as a flocculant has become a topic of interest over the years due to its positively charged polymer and biodegradable and non-toxic properties. However, most studies only focus on microalgae and wastewater treatment. This study provides crucial insight into the potential of using chitosan as an organic flocculant to harvest lipids and docosahexaenoic acid (DHA-rich Aurantiochytrium sp. SW1 cells by examining the correlation of flocculation parameters (chitosan concentration, molecular weight, medium pH, culture age, and cell density) toward the flocculation efficiency and zeta potential of the cells. A strong correlation between the pH and harvesting efficiency was observed as the pH increased from 3, with the optimal flocculation efficiency of >95% achieved at a chitosan concentration of 0.5 g/L at pH 6 where the zeta potential was almost zero (3.26 mV). The culture age and chitosan molecular weight have no effect on the flocculation efficiency but increasing the cell density decreases the flocculation efficiency. This is the first study to reveal the potential of chitosan to be used as a harvesting alternative for thraustochytrid cells. [ABSTRACT FROM AUTHOR]

Microalgae represent promising sources of bioactive compounds for pharmaceutical and industrial applications. The emergence of antibiotic resistant bacteria leads to the need to explore new cost-effective, safe, and potent bioactive compounds from the microalgae. This study aimed to investigate the potential of local microalgae for their antimicrobial properties and bioactive compounds. Three local microalgae namely Chlorella sorokiniana (UKM2), Chlorella sp. UKM8, and Scenedesmus sp. UKM9 biomass methanol extracts (ME) were prepared and tested against Gram-positive and Gram-negative bacteria. Chlorella sp. UKM8-ME showed the highest antibacterial activity. UKM8-ME minimum inhibitory concentrations were in the range of 0.312 to 6.25 mg/mL. Cytotoxicity evaluation using MTT assay showed that the microalgae methanolic extracts did not exhibit cytotoxicity against Vero-cells. The UKM8-ME was mainly containing 28 compounds from the Gas Chromatography-Mass Spectrometry (GC–MS) analysis. Major compounds of UKM8-ME included phenol (18.5%), hexadecanoic acid (18.25%), phytol (14.43%), 9,12-octadecadienoic acid (13.69%), and bicyclo[3.1.1]heptane (7.23%), which have been previously described to possess antimicrobial activity. Hence, Chlorella sp. (UKM8) methanol extracts showed promising antibacterial activity. More comprehensive studies are required to purify these antimicrobial compounds and develop our understanding on their mechanism in UKM8-ME to unleash their specific potential. [ABSTRACT FROM AUTHOR]

Microalgae cultivation in open ponds requires a large footprint, while most photobioreactors need improvement in the ratio of surface to volume and energy consumption. In this study, polyethersulfone (PES) and poly(vinylidene fluoride) (PVDF) hollow fiber membranes with a large surface area were rearranged into open-ended and dead-ended configurations to improve the air-liquid interface cultivation of Navicula incerta. N. incerta were successfully grown on the porous membrane surface with the nutrients circulating inside the lumen. Fourier-transform infrared spectra showed the accumulation of polysaccharides, proteins and humic acids. Hydrophilic polysaccharides reduced water contact angles on PES and PVDF membranes to 37.2 ± 2.6° and 55.7 ± 3.3°, respectively. However, the porosity of PES (80.1 ± 1.1%) and PVDF (61.3 ± 4.5%) membranes were not significantly affected even after cultivation and harvesting of N. incerta. Scanning electron images further confirmed that N. incerta , cell debris and extracellular organic matter accumulated on the membrane. With large pores and a hydrophobic surface, PVDF hollow fiber membranes offered a greater improvement in N. incerta cell growth rate compared to PES hollow fiber membranes despite using different configurations. In the dead-ended configuration, they even attained the greatest improvement in N. incerta growth rate, up to 54.0%. However, PES hollow fiber membranes only achieved improvement in harvesting efficiency within the range of 18.7–38.0% due to weak cell adhesion. PVDF hollow fiber membranes significantly promoted the growth of microalgae N. incerta through the air-liquid interface system, leading to potential applications in wastewater treatment. [Display omitted] • PES and PVDF hollow fiber membranes were rearranged for the air-liquid interface cultivation. • Microalgae grew on the membrane surface with the nutrients circulating inside the lumen. • Membrane porosity remained after cultivation and harvesting through air purge. • PVDF membrane with large pores and a hydrophobic surface promoted cell growth rate. • The dead-ended configuration attained an improvement of 54.0% in cell growth rate. [ABSTRACT FROM AUTHOR]