27 results on '"Di Gioia, Diana"'
Search Results
2. Beneficial Bacteria and Plant Extracts Promote Honey Bee Health and Reduce Nosema ceranae Infection
- Author
-
Garrido, Paula Melisa, Porrini, Martín Pablo, Alberoni, Daniele, Baffoni, Loredana, Scott, Dara, Mifsud, David, Eguaras, Matín Javier, and Di Gioia, Diana
- Published
- 2024
- Full Text
- View/download PDF
3. Pot experimental trial for assessing the role of different composts on decontamination and reclamation of a polluted soil from an illegal dump site in Southern Italy using Brassica juncea and Sorghum bicolor
- Author
-
Mazzon, Martina, Bozzi Cionci, Nicole, Buscaroli, Enrico, Alberoni, Daniele, Baffoni, Loredana, Di Gioia, Diana, Marzadori, Claudio, Barbanti, Lorenzo, Toscano, Attilio, and Braschi, Ilaria
- Published
- 2024
- Full Text
- View/download PDF
4. From food waste to eco-friendly functionalized polymer composites: Investigation of orange peels as active filler
- Author
-
Pagliarini, Elia, Minichiello, Carmen, Sisti, Laura, Totaro, Grazia, Baffoni, Loredana, Di Gioia, Diana, and Saccani, Andrea
- Published
- 2024
- Full Text
- View/download PDF
5. The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use
- Author
-
Fernandez De Landa, Gregorio, Alberoni, Daniele, Baffoni, Loredana, Fernandez De Landa, Mateo, Revainera, Pablo Damian, Porrini, Leonardo Pablo, Brasesco, Constanza, Quintana, Silvina, Zumpano, Francisco, Eguaras, Martìn Javier, Maggi, Matias Daniel, and Di Gioia, Diana
- Published
- 2023
- Full Text
- View/download PDF
6. Alterations in the Microbiota of Caged Honeybees in the Presence of Nosema ceranae Infection and Related Changes in Functionality
- Author
-
Alberoni, Daniele, Di Gioia, Diana, and Baffoni, Loredana
- Published
- 2023
- Full Text
- View/download PDF
7. The fate of bacteria in urban wastewater-irrigated peach tree: a seasonal evaluation from soil to canopy
- Author
-
Perulli, Giulio Demetrio, Gaggia, Francesca, Manfrini, Luigi, Di Gioia, Diana, Toscano, Attilio, and Morandi, Brunella
- Published
- 2024
- Full Text
- View/download PDF
8. Combined effect of a neonicotinoid insecticide and a fungicide on honeybee gut epithelium and microbiota, adult survival, colony strength and foraging preferences
- Author
-
Favaro, Riccardo, Garrido, Paula Melisa, Bruno, Daniele, Braglia, Chiara, Alberoni, Daniele, Baffoni, Loredana, Tettamanti, Gianluca, Porrini, Martin Pablo, Di Gioia, Diana, and Angeli, Sergio
- Published
- 2023
- Full Text
- View/download PDF
9. Valorization of coffee wastes as plant growth promoter in mulching film production: A contribution to a circular economy
- Author
-
Pagliarini, Elia, Totaro, Grazia, Saccani, Andrea, Gaggìa, Francesca, Lancellotti, Isabella, Di Gioia, Diana, and Sisti, Laura
- Published
- 2023
- Full Text
- View/download PDF
10. The ground beetle Pseudoophonus rufipes gut microbiome is influenced by the farm management system
- Author
-
Magagnoli, Serena, Alberoni, Daniele, Baffoni, Loredana, Martini, Antonio, Marini, Francesca, Di Gioia, Diana, Mazzon, Martina, Marzadori, Claudio, Campanelli, Gabriele, and Burgio, Giovanni
- Published
- 2022
- Full Text
- View/download PDF
11. Study protocol on the safety and feasibility of a normocaloric ketogenic diet in people with amyotrophic lateral sclerosis
- Author
-
De Marchi, Fabiola, Collo, Alessandro, Scognamiglio, Ada, Cavaletto, Maria, Bozzi Cionci, Nicole, Biroli, Giampaolo, Di Gioia, Diana, Riso, Sergio, and Mazzini, Letizia
- Published
- 2022
- Full Text
- View/download PDF
12. The Gut Microbiome of Two Wild Bumble Bee Species Native of South America: Bombus pauloensis and Bombus bellicosus.
- Author
-
Fernandez de Landa, Gregorio, Alberoni, Daniele, Braglia, Chiara, Baffoni, Loredana, Fernandez de Landa, Mateo, Revainera, Pablo Damian, Quintana, Silvina, Zumpano, Francisco, Maggi, Matias Daniel, and Di Gioia, Diana
- Subjects
BUMBLEBEES ,GUT microbiome ,NOSEMA ceranae ,AGRICULTURE ,POLLINATORS ,BEE colonies - Abstract
South America is populated by a wide range of bumble bee species that represent an important source of biodiversity, supporting pollination services in natural and agricultural ecosystems. These pollinators provide unique specific microbial niches, populated by a wide number of microorganisms such as symbionts, environmental opportunistic bacteria, and pathogens. Recently, it was demonstrated how microbial populations are shaped by trophic resources and environmental conditions but also by anthropogenic pressure, which strongly affects microbes' functionality. This study is focused on the impact of different land uses (natural reserve, agroecosystem, and suburban) on the gut microbiome composition of two South American bumble bees, Bombus pauloensis and Bombus bellicosus. Gut microbial DNA extracted from collected bumble bees was sequenced on the Illumina MiSeq platform and correlated with land use. Nosema ceranae load was analyzed with qPCR and correlated with microbiome data. Significant differences in gut microbiome composition between the two wild bumble bee species were highlighted, with notable variations in α- and β-diversity across study sites. Bombus bellicosus showed a high abundance of Pseudomonas, a genus that includes environmental saprobes, and was found to be the second major taxa populating the gut microbiome, probably indicating the vulnerability of this host to environmental pollution. Pathogen analysis unveils a high prevalence of N. ceranae, with B. bellicosus showing higher susceptibility. Finally, Gilliamella exhibited a negative correlation with N. ceranae, suggesting a potential protective role of this commensal taxon. Our findings underscore the importance of considering microbial dynamics in pollinator conservation strategies, highlighting potential interactions between gut bacteria and pathogens in shaping bumble bee health. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees?
- Author
-
Braglia, Chiara, Alberoni, Daniele, Garrido, Paula Melisa, Porrini, Martin Pablo, Baffoni, Loredana, Scott, Dara, Eguaras, Martin Javier, Di Gioia, Diana, and Mifsud, David
- Subjects
SERRATIA ,HONEYBEES ,SERRATIA marcescens ,BEES ,VARROA destructor ,GENE targeting ,BIFIDOBACTERIUM - Abstract
The genus Serratia harbors opportunistic pathogenic species, among which Serratia marcescens is pathogenic for honeybees although little studied. Recently, virulent strains of S. marcescens colonizing the Varroa destructor mite's mouth were found vectored into the honeybee body, leading to septicemia and death. Serratia also occurs as an opportunistic pathogen in the honeybee's gut with a low absolute abundance. The Serratia population seems controlled by the host immune system, but its presence may represent a hidden threat, ready to arise when honeybees are weakened by biotic and abiotic stressors. To shed light on the Serratia pathogen, this research aims at studying Serratia's development dynamics in the honeybee body and its interactions with the co-occurring fungal pathogen Vairimorpha ceranae. Firstly, the degree of pathogenicity and the ability to permeate the gut epithelial barrier of three Serratia strains, isolated from honeybees and belonging to different species (S. marcescens, Serratia liquefaciens, and Serratia nematodiphila), were assessed by artificial inoculation of newborn honeybees with different Serratia doses (104, 106, and 108 cells/mL). The absolute abundance of Serratia in the gut and in the hemocoel was assessed in qPCR with primers targeting the luxS gene. Moreover, the absolute abundance of Serratia was assessed in the gut of honeybees infected with V. ceranae at different development stages and supplied with beneficial microorganisms and fumagillin. Our results showed that all tested Serratia strains could pass through the gut epithelial barrier and proliferate in the hemocoel, with S. marcescens being the most pathogenic. Moreover, under cage conditions, Serratia better proliferates when a V. ceranae infection is co-occurring, with a positive and significant correlation. Finally, fumagillin and some of the tested beneficial microorganisms could control both Serratia and Vairimorpha development. Our findings suggest a correlation between the two pathogens under laboratory conditions, a co-occurring infection that should be taken into consideration by researches when testing antimicrobial compounds active against V. ceranae, and the related honeybees survival rate. Moreover, our findings suggest a positive control of Serrata by the environmental microorganism Apilactobacillus kunkeei in a in vivo model, confirming the potential of this specie as beneficial bacteria for honeybees. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. A first approach in the correlation of pathogens load affecting Bombus pauloensis to the land use in Buenos Aires Province.
- Author
-
Fernandez de Landa, Gregorio, Revainera, Pablo D., Alberoni, Daniele, Nicolli, Anabella R., Fernandez de Landa, Mateo, Zumpano, Francisco, Brasesco, Constanza, Reynaldi, Francisco J., Quintana, Silvina, Petrigh, Romina, Di Gioia, Diana, Galetto, Leonardo, Eguaras, Martín J., and Maggi, Matias D.
- Abstract
Bombus pauloensis is a native bumble bee species widely distributed over South America and a key pollinator for native plants and commercial crops. This species is affected by pathogens such as Nosema ceranae, Crithidia bombi and Apis mellifera Filamentous Virus (AmFV). This work aims to document the presence and intensity of exogenous pathogens on the native bumble bee B. pauloensis in different periods of the year during spring and summer. Bumble bees were sampled in four study areas with contrasting land uses to preliminary evaluate if anthropization levels can be related to the presence and intensity of pathogens. DNA was isolated from twenty individuals per sampling site and N. ceranae, C. bombi and AmFV pathogens load were quantified by quantitative PCR. The results showed a wide and ubiquitous prevalence of N. ceranae and C. bombi pathogens in all the sampled bumble bees throughout the year, with a pathogens load that did not differ significantly among the sampling sites. AmFV was undetected in any of the individuals analyzed in any sampling site. This suggests that human activities could equally impact all habitats populated by B. pauloensis. Honey bees were detected with a relevant abundance in all the sampling sites and could be one of the main anthropogenic drivers of pathogens spread and host switches in the analyzed sampling sites. This study analyzes the general occurrence of the prevalence and intensity of the pathogens in a native Argentinian bumble bee species and attempts the correlation with the land use [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
15. Application of an Exhausted Fermentation Broth Obtained from Biohydrogen Production in an Apple Orchard: Assessment of Fruit Quality.
- Author
-
Galiano, Michele, Perulli, Giulio Demetrio, Morandi, Brunella, and Di Gioia, Diana
- Subjects
FRUIT quality ,APPLE orchards ,ORCHARDS ,FERMENTATION ,SUSTAINABILITY ,FRUIT ripening - Abstract
About 95% of global hydrogen production is made by fossil fuels using different technologies which are all characterized by high energy consumption and high carbon emissions. Alternatively, more sustainable production methods, such as biological fermentation processes, are under study. Dark fermentation, also called acidogenesis, entails the transformation of a great variety of organic substances into a mixture of organic and inorganic products, as well as gases (H
2 and CO2 ). In this study we tested an exhausted fermentation broth, derived after Clostridium fermentation for H2 production, as a biostimulant via foliar application in an intensive apple orchard. Two different doses were applied upon dilution of the broth in water (100 mL L−1 and 10 mL L−1 ), evaluating the main fruit quality parameters (fresh weight, fruit diameter, dry matter, firmness, soluble solid content, color lightness, DA index) in addition to macro- and micro-nutrients and heavy metals concentrations. Chemical characterization of the broth showed a high amount of low-MW polypeptides (Trp-Glu-Lys, Ile-Pro-Ile, Phe-Pro-Lys, His-Pro) and organic acids (formic acid, butyric acid, butanedioic acid); moreover, quantitative analyses of inorganic ions showed no heavy metal detection but high concentrations of nitrogen, phosphorus and potassium, compatible with use in agriculture. The fruit quality parameters showed significantly higher mean fruit weight compared to the untreated trees, as well as higher dry matter. No statistical differences were recorded among the treatments for fruit firmness, diameter and yield. Soluble solids content in both treatments were significantly lower than the controls, whereas the DA index mean values were higher in both treatments compared to the controls, indicating a delay in fruit ripening probably due to the high nitrogen broth concentration. Regarding the chemical analyses of fruits, no particular differences were found among the treatments, except for Fe, which showed a significantly higher amount upon treatment with the lower dose. As concerns leaves, no phytotoxic symptoms were detected in both treatments, making the described exhausted broth a candidate for its use as a plant biostimulant. Additional studies are needed to evaluate the ideal application dose, identify further action targets and implement appropriate strategies to concentrate the biostimulant active compounds. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
16. Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri.
- Author
-
Gaggìa, Francesca, Jakobsen, Rasmus Riemer, Alberoni, Daniele, Baffoni, Loredana, Cutajar, Simone, Mifsud, David, Nielsen, Dennis Sandris, and Di Gioia, Diana
- Subjects
BEES ,HONEYBEES ,REPRODUCTIVE isolation ,GUT microbiome ,MICROBIAL communities ,BACTERIAL genes ,LACTOBACILLACEAE ,COLD adaptation - Abstract
Introduction: Apis mellifera evolved mainly in African, Asian, and European continents over thousands of years, leading to the selection of a considerable number of honey bees subspecies that have adapted to various environments such as hot semi-desert zones and cold temperate zones. With the evolution of honey bee subspecies, it is possible that environmental conditions, food sources, and microbial communities typical of the colonized areas have shaped the honey bee gut microbiota. Methods: In this study the microbiota of two distinct lineages (mitochondrial haplotypes) of bees Apis mellifera ruttneri (lineage A) and Apis mellifera ligustica and carnica (both lineage C) were compared. Honey bee guts were collected in a dry period in the respective breeding areas (the island of Malta and the regions of Emilia-Romagna and South Tyrol in Italy). Microbial DNA from the honey bee gut was extracted and amplified for the V3-V4 regions of the 16S rRNA gene for bacteria and for ITS2 for fungi. Results: The analyses carried out show that the Maltese lineage A honey bees have a distinctive microbiota when compared to Italian lineage C honey bees, with the most abundant genera being Bartonellaceae and Lactobacillaceae, respectively. Lactobacillaceae in Maltese Lineage A honey bees consist mainly of Apilactobacillus instead of Lactobacillus and Bombilactobacillus in the lineage C. Lineage A honey bee gut microbiota also harbors higher proportions of Arsenophonus, Bombella, Commensalibacter, and Pseudomonas when compared to lineage C. Discussion: The environment seems to be the main driver in the acquisition of these marked differences in the gut microbiota. However, the influence of other factors such as host genetics, seasonality or geography may still play a significant role in the microbiome shaping, in synergy with the environmental aspects. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
17. Yield and Nutraceutical Value of Lettuce and Basil Improved by a Microbial Inoculum in Greenhouse Experiments.
- Author
-
Pagliarini, Elia, Gaggìa, Francesca, Quartieri, Maurizio, Toselli, Moreno, and Di Gioia, Diana
- Subjects
LETTUCE ,GREENHOUSES ,BASIL ,BACILLUS (Bacteria) ,PLANT growth ,COPPER - Abstract
Members of Bacillus spp. have been widely used to enrich the soil/root interface to provide plant growth promoting activities. A new isolate, namely to Bacillus sp. VWC18, has been tested under greenhouse conditions in lettuce (Lactuca sativa L.) pots at different concentrations (10
3 , 105 , 107 , and 109 CFU·mL−1 ) and application time (single inoculum at transplant and multiple inoculum every ten days) to evaluate the best application dose and frequency. Analysis of foliar yield, main nutrients, and minerals evidenced a significant response for all applications. The lowest (103 CFU·mL−1 ) and the highest doses (109 CFU·mL−1 ), applied every ten days until harvest, had the greatest efficacy; the nutrient yield (N, K, P, Na, Ca, Fe, Mg, Mn, Cu, and B) increased more than twice. A new randomized block design with three replicates was then performed in lettuce and basil (Ocinum basilicum L.), with the two best performing concentrations applied every ten days. In addition to previous analysis, root weight, chlorophyll, and carotenoids were also examined. Both experiments confirmed the previous results: inoculation of the substrate with Bacillus sp. VWC18 promoted plant growth, chlorophyll, and mineral uptake in both crop species. Root weight duplicated or triplicated compared to control plants, and chlorophyll concentration reached even higher values. Both parameters had a dose-dependent increase. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
18. Millet Fermented by Different Combinations of Yeasts and Lactobacilli: Effects on Phenolic Composition, Starch, Mineral Content and Prebiotic Activity.
- Author
-
Balli, Diletta, Cecchi, Lorenzo, Pieraccini, Giuseppe, Venturi, Manuel, Galli, Viola, Reggio, Marta, Di Gioia, Diana, Furlanetto, Sandra, Orlandini, Serena, Innocenti, Marzia, and Mulinacci, Nadia
- Subjects
MILLETS ,STARCH ,PEARL millet ,YEAST ,ENZYMES ,CORNSTARCH ,LACTOBACILLUS ,INULIN - Abstract
Millet is the sixth-highest yielding grain in the world and a staple crop for millions of people. Fermentation was applied in this study to improve the nutritional properties of pearl millet. Three microorganism combinations were tested: Saccharomyces boulardii (FPM1), Saccharomyces cerevisiae plus Campanilactobacillus paralimentarius (FPM2) and Hanseniaspora uvarum plus Fructilactobacillus sanfranciscensis (FPM3). All the fermentation processes led to an increase in minerals. An increase was observed for calcium: 254 ppm in FPM1, 282 ppm in FPM2 and 156 ppm in the unfermented sample. Iron increased in FPM2 and FPM3 (approx. 100 ppm) with respect the unfermented sample (71 ppm). FPM2 and FPM3 resulted in richer total phenols (up to 2.74 mg/g) compared to the unfermented sample (2.24 mg/g). Depending on the microorganisms, it was possible to obtain different oligopeptides with a mass cut off ≤10 kDalton that was not detected in the unfermented sample. FPM2 showed the highest resistant starch content (9.83 g/100 g) and a prebiotic activity on Bifidobacterium breve B632, showing a significant growth at 48 h and 72 h compared to glucose (p < 0.05). Millet fermented with Saccharomyces cerevisiae plus Campanilactobacillus paralimentarius can be proposed as a new food with improved nutritional properties to increase the quality of the diet of people who already use millet as a staple food. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. Characterization of Lactic Acid Bacteria Isolated from Spontaneously Fermented Sausages: Bioprotective, Technological and Functional Properties.
- Author
-
Nikodinoska, Ivana, Tabanelli, Giulia, Baffoni, Loredana, Gardini, Fausto, Gaggìa, Francesca, Barbieri, Federica, and Di Gioia, Diana
- Subjects
LACTIC acid bacteria ,SAUSAGES ,OSMOTIC pressure ,CLOSTRIDIA ,DRUG resistance in bacteria ,LISTERIA monocytogenes - Abstract
Fermentation is one of the most ancient strategies to improve safety and extend shelf-life of the products. Starter cultures are mainly represented by lactic acid bacteria (LAB), which may also be bioprotective agents controlling the fermentation process, the native microbiota and pathogen outgrowth. This work aimed to select new LAB strains from spontaneously fermented sausages produced in different areas of Italy, which can be effective as starter cultures and bioprotective agents in fermented salami. The strains, mainly belonging to the Latilactobacillus sakei species, were characterized for their ability to inhibit major meat pathogens, the presence of antibiotic resistances and amine production. Moreover, technological performances, such as growth and acidification kinetics at increasing NaCl concentrations, were studied. As a result, new autochthonous Lat. sakei strains were obtained, lacking antibiotic resistance, possessing antimicrobial activity against Clostridium sporogenes, Listeria monocytogenes, Salmonella and Escherichia coli and with high growth performance under osmotic pressure. These strains have the potential for future application to improve the safety of fermented meats, even under conditions in which chemical preservatives are reduced or eliminated. Moreover, studies on autochthonous cultures are pivotal for guaranteeing specific characteristics of traditional products that represent an important cultural heritage. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
20. In-process real-time probiotic phenotypic strain identity tracking: The use of Fourier transform infrared spectroscopy.
- Author
-
Deidda, Francesca, Cordovana, Miriam, Cionci, Nicole Bozzi, Graziano, Teresa, Di Gioia, Diana, and Pane, Marco
- Abstract
Probiotic bacteria, capable of conferring benefits to the host, can present challenges in design, development, scale-up, manufacturing, commercialization, and life cycle management. Strain identification is one of the main quality parameters; nevertheless, this task can be challenging since established methodologies can lack resolution at the strain level for some microorganisms and\or are labor-intensive and time-consuming. Fourier transform infrared spectroscopy (FTIRS) has been largely used for the investigation of pathogenic species in the clinical field, whereas only recently has been proposed for the identification of probiotic strains. Within the probiotic industrial production, bacterial strains can be subjected to stressful conditions that may affect genomic and phenotypic characteristics; therefore, real-time monitoring of all the sequential growth steps is requested. Considering the fast, low-cost, and high-throughput features, FTIRS is an innovative and functional technology for typing probiotic strains from benchtop experiments to large-scale industrial production, allowing the monitoring of stability and identity of probiotic strains. In this study, the discriminatory power of FTIRS was assessed for four Lactiplantibacillus plantarum probiotic strains grown under different conditions, including temperatures (30 and 37◦C) and medium (broth and agar), after consecutive sub-culturing steps. A comparison between the generated spectra with pulsed-field gel electrophoresis (PFGE) profiles was also performed. FTIRS was not only able to distinguish the strains of L. plantarum under different growth conditions but also to prove the phenotypic stability of L. plantarum type strain LP-CT after six growing steps. Regardless of the growth conditions, FTIRS spectra related to LP-CT constituted a unique hierarchical cluster, separated from the other L. plantarum strains. These results were confirmed by a PFGE analysis. In addition, based on FTIRS data, broth cultures demonstrated a higher reproducibility and discriminatory power with respect to agar ones. These results support the introduction of FTIRS in the probiotic industry, allowing for the step-by-step monitoring of massive microbial production while also guaranteeing the stability and purity of the probiotic strain. The proposed novel approach can constitute an impressive improvement in the probiotic manufacturing process. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
21. Selection of Acetic Acid Bacterial Strains and Vinegar Production From Local Maltese Food Sources.
- Author
-
Mizzi, Joseph, Gaggìa, Francesca, Cionci, Nicole Bozzi, Di Gioia, Diana, and Attard, Everaldo
- Subjects
ACETIC acid ,VINEGAR ,LOCAL foods ,ACETOBACTER ,OPUNTIA ,PRODUCE trade - Abstract
This study investigates the isolation, identification, and fermentation performance of autochthonous acetic acid bacteria (AAB) from local niche habitats on the Island of Gozo (Malta) and their further use for vinegar production, employing local raw materials. The bacteria were isolated from grapevine berries and vinegar produced in the cottage industry. Following phenotype and genotype identification, the AAB were ascribed to the genera Acetobacter, Gluconobacter, and Komagataeibacter. A mixture of selected AAB was tested as an inoculum for vinegar production in bench fermenters, under different conditions and substrates, namely, grapes, honey, figs, onions, prickly pear, and tomatoes. The bench fermenters were operated under semi-continuous fermentation where working volumes were maintained by discharging and subsequent recharging accordingly to maintain the acidity in fermenters by adding 30--50 g/l of acetic acid for optimal Acetobacteraceae performance. Finally, the vinegar products obtained from the different substrates were evaluated for their quality, including organoleptic properties, which showed the superior quality of wood-treated vinegar samples with respect to neat vinegar samples. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. Value Propositions for Improving the Competitiveness of Short Food Supply Chains Built on Technological and Non-Technological Innovations.
- Author
-
SEBÖK, ANDRÁS, VARSANYI, KINGA, KUJANI, KATALIN, XHAKOLLARI, VILMA, FRICZ, AGNES SZEGEDYNE, CASTELLINI, ALESSANDRA, DI GIOIA, DIANA, GAGGIA, FRANCESCA, and CANAVARI, MAURIZIO
- Subjects
SUPPLY chains ,FOOD supply ,FOOD chains ,VALUE proposition ,CONSUMPTION (Economics) ,TECHNOLOGICAL innovations - Abstract
There has been growing consumer demand for the products and services of the short food supply chains (SFSCs) in recent times. A procedure was developed to identify the technological and nontechnological innovations that can improve the performance and competitiveness of the SFSCs. The needs of the SFSCs for innovative solutions were collected by interviewing 18 SFSCs from 9 countries. An inventory was prepared to contain 136 technological and non-technological innovations, meeting these needs. The innovations were collected from the good practices of the 18 SFSCs, experiences of the project partners and state of the art. The success factors and bottlenecks of each short food supply chain operation and their current value propositions were identified. From the inventory, those innovations were selected for each short food chain case study which can be applied to eliminate or reduce the bottlenecks or enhance the success factors leading to new, upgraded value propositions with increased added value for the consumers. The new, upgraded value propositions can serve as a starting point for developing a strategy for improving the competitiveness of a short food chain organisation through the application of innovations. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
23. Durability of biopolymeric composites formulated with fillers from a by‐product of coffee roasting.
- Author
-
Sisti, Laura, Totaro, Grazia, Rosato, Antonella, Bozzi Cionci, Nicole, Di Gioia, Diana, Barbieri, Luisa, and Saccani, Andrea
- Subjects
POLYMERIC composites ,COFFEE beans ,ROASTING (Cooking) ,COFFEE ,ELASTICITY ,ELASTIC modulus ,PLANT polyphenols - Abstract
Particulate composites based on poly(lactic acid) (PLA) and poly(butylene succinate) (PBS) biopolymers have been formulated. Silver skin (SSK), the by‐product derived from the roasting of coffee beans, has been milled to micrometric size and added up to a 30 wt% of loading, without any previous treatment. The dynamic mechanical properties of the composites have been investigated, as well as the stability toward UV and IR radiation. The filler improves some mechanical properties such as the elastic modulus (E′) mostly in PBS. Photoaging experiments demonstrate that SSK, due to its polyphenols content and antioxidant activity, is able to protect both PLA and PBS from UV degradation, highlighting an added value of SSK waste‐based composites. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
24. Antibiotic Exposure, Common Morbidities and Main Intestinal Microbial Groups in Very Preterm Neonates: A Pilot Study.
- Author
-
Bozzi Cionci, Nicole, Lucaccioni, Laura, Pietrella, Elisa, Ficara, Monica, Spada, Caterina, Torelli, Paola, Bedetti, Luca, Lugli, Licia, Di Gioia, Diana, and Berardi, Alberto
- Subjects
NEONATAL sepsis ,ANTIBIOTICS ,PREMATURE infants ,NEWBORN infants ,BACTEROIDES fragilis ,PATENT ductus arteriosus - Abstract
Prematurity exposes newborns to increased risks of infections and it is associated with critical morbidities. Preterm infants often require antibiotic therapies that can affect the correct establishment of gut microbiota. The aim of this study was to investigate targeted intestinal bacteria in preterm neonates with common morbidities and receiving antibiotic treatments of variable duration. Stool samples were collected after birth, at 15, 30 and 90 days of life. qPCR quantification of selected microbial groups (Bifidobacterium spp., Bacteroides fragilis group, Enterobacteriaceae, Clostridium cluster I and total bacteria) was performed and correlation between their levels, the duration of antibiotic treatment and different clinical conditions was studied. An increasing trend over time was observed for all microbial groups, especially for Bifdobacterium spp. Prolonged exposure to antibiotics in the first weeks of life affected Clostridium and B. fragilis levels, but these changes no longer persisted at 90 days of life. Variations of bacterial counts were associated with the length of hospital stay, feeding and mechanical ventilation. Late-onset sepsis and patent ductus arteriosus reduced the counts of Bifidobacterium, whereas B. fragilis was influenced by compromised respiratory conditions. This study can be a start point for the identification of microbial biomarkers associated with some common morbidities and tailored strategies for a healthy microbial development. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
25. Antibiotic Exposure, Common Morbidities and Main Intestinal Microbial Groups in Very Preterm Neonates: A Pilot Study
- Author
-
Nicole Bozzi Cionci, Laura Lucaccioni, Elisa Pietrella, Monica Ficara, Caterina Spada, Paola Torelli, Luca Bedetti, Licia Lugli, Diana Di Gioia, Alberto Berardi, Bozzi Cionci, Nicole, Lucaccioni, Laura, Pietrella, Elisa, Ficara, Monica, Spada, Caterina, Torelli, Paola, Bedetti, Luca, Lugli, Licia, Di Gioia, Diana, and Berardi, Alberto
- Subjects
Microbiology (medical) ,gut microbiota ,Neonates ,Gut microbiota ,Biochemistry ,Microbiology ,Antibiotic exposure ,Infectious Diseases ,antibiotic exposure ,Preterm ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,neonate ,neonates ,preterm - Abstract
Prematurity exposes newborns to increased risks of infections and it is associated with critical morbidities. Preterm infants often require antibiotic therapies that can affect the correct establishment of gut microbiota. The aim of this study was to investigate targeted intestinal bacteria in preterm neonates with common morbidities and receiving antibiotic treatments of variable duration. Stool samples were collected after birth, at 15, 30 and 90 days of life. qPCR quantification of selected microbial groups (Bifidobacterium spp., Bacteroides fragilis group, Enterobacteriaceae, Clostridium cluster I and total bacteria) was performed and correlation between their levels, the duration of antibiotic treatment and different clinical conditions was studied. An increasing trend over time was observed for all microbial groups, especially for Bifdobacterium spp. Prolonged exposure to antibiotics in the first weeks of life affected Clostridium and B. fragilis levels, but these changes no longer persisted at 90 days of life. Variations of bacterial counts were associated with the length of hospital stay, feeding and mechanical ventilation. Late-onset sepsis and patent ductus arteriosus reduced the counts of Bifidobacterium, whereas B. fragilis was influenced by compromised respiratory conditions. This study can be a start point for the identification of microbial biomarkers associated with some common morbidities and tailored strategies for a healthy microbial development.
- Published
- 2022
26. In-process real-time probiotic phenotypic strain identity tracking: The use of Fourier transform infrared spectroscopy
- Author
-
Francesca Deidda, Miriam Cordovana, Nicole Bozzi Cionci, Teresa Graziano, Diana Di Gioia, Marco Pane, Deidda, Francesca, Cordovana, Miriam, Bozzi Cionci, Nicole, Graziano, Teresa, Di Gioia, Diana, and Pane, Marco
- Subjects
Microbiology (medical) ,FTIR spectroscopy ,large-scale production ,Lactiplantibacillus plantarum ,strain typing ,Microbiology ,IR Biotyper® ,probiotic - Abstract
Probiotic bacteria, capable of conferring benefits to the host, can present challenges in design, development, scale-up, manufacturing, commercialization, and life cycle management. Strain identification is one of the main quality parameters; nevertheless, this task can be challenging since established methodologies can lack resolution at the strain level for some microorganisms and\or are labor-intensive and time-consuming. Fourier transform infrared spectroscopy (FTIRS) has been largely used for the investigation of pathogenic species in the clinical field, whereas only recently has been proposed for the identification of probiotic strains. Within the probiotic industrial production, bacterial strains can be subjected to stressful conditions that may affect genomic and phenotypic characteristics; therefore, real-time monitoring of all the sequential growth steps is requested. Considering the fast, low-cost, and high-throughput features, FTIRS is an innovative and functional technology for typing probiotic strains from bench-top experiments to large-scale industrial production, allowing the monitoring of stability and identity of probiotic strains. In this study, the discriminatory power of FTIRS was assessed for four Lactiplantibacillus plantarum probiotic strains grown under different conditions, including temperatures (30 and 37°C) and medium (broth and agar), after consecutive sub-culturing steps. A comparison between the generated spectra with pulsed-field gel electrophoresis (PFGE) profiles was also performed. FTIRS was not only able to distinguish the strains of L. plantarum under different growth conditions but also to prove the phenotypic stability of L. plantarum type strain LP-CT after six growing steps. Regardless of the growth conditions, FTIRS spectra related to LP-CT constituted a unique hierarchical cluster, separated from the other L. plantarum strains. These results were confirmed by a PFGE analysis. In addition, based on FTIRS data, broth cultures demonstrated a higher reproducibility and discriminatory power with respect to agar ones. These results support the introduction of FTIRS in the probiotic industry, allowing for the step-by-step monitoring of massive microbial production while also guaranteeing the stability and purity of the probiotic strain. The proposed novel approach can constitute an impressive improvement in the probiotic manufacturing process.
- Published
- 2022
27. Changes in microbiota and functional properties in anthropogenic soil
- Author
-
Zou, Yukun <1984> and Di Gioia, Diana
- Subjects
AGR/16 Microbiologia agraria ,complex mixtures - Abstract
Since 1993, when Muyzer et al., used denaturing gradient gel electrophoresis (DGGE) to detect environmental soil microbial community for the very first time, it opened a brand-new page for soil microbial ecologist. He offered us an opportunity to explore the unculturable microorganisms which accounted for above 90% lived in soil. More and more scientists concerned this subject about soil health and function of soil microorganisms, not only because the microorganisms played key roles in many soil biochemical elements cycling but also understanding their working mechanisms will help us to build a sustainable soil ecosystem in this full of limited resources planet. This thesis is focused on the differently managed soil that how the soil quality changed and soil microbiota response to the different management. The main background of the Chapter 2 is the total organic carbon loss caused by the conversion of rainforests into agricultural plantations. The main background of Chapter 3 and 4 is returning something back to the soil, such as composts and pyrogenic organic matter. So, in both cases the total organic carbon is increased. In all the cases, we focused on soil properties, bacteria community, fungi community, their interrelationships, and consequences. The results obtained in this thesis, first, it will provide a mechanistic understanding of carbon and nitrogen losses from the conversion of rainforests to agricultural plantations and the roles of microbial communities in the decomposition of soil organic matter and nitrogen cycling. Secondly, we will understand better the relative importance of biotic and abiotic factors who contribute more to soil respiration after different nitrogen sources addition. Thirdly, we could expand our knowledge about long-term large charcoal addition to soil impact on soil environment and soil microbial community and gain a valuable model for biochar ageing effect in soil.
- Published
- 2021
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.