1. Long-Lasting Silver Nanoparticles Synthesized with Tagetes erecta and Their Antibacterial Activity against Erwinia amylovora, a Serious Rosaceous Pathogen.
- Author
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Zarate-Escobedo, Johana, Zavaleta-Mancera, Hilda Araceli, Soto-Hernández, Ramón Marcos, Pérez-Rodríguez, Paulino, Vilchis-Nestor, Alfredo Rafael, Silva-Rojas, Hilda Victoria, and Trejo-Téllez, Libia Iris
- Subjects
ERWINIA ,ERWINIA amylovora ,SILVER nanoparticles ,ANTIBACTERIAL agents ,TANNINS ,MARIGOLDS ,PHYTOCHEMICALS ,ELECTRON microscope techniques - Abstract
A rapid, eco-friendly, and simple method for the synthesis of long-lasting (2 years) silver nanoparticles (AgNPs) is reported using aqueous leaf and petal extracts of Tagetes erecta L. The particles were characterized using UV-Visible spectrophotometry and the analytical and crystallographic techniques of transmission electron microscopy (TEM). The longevity of the AgNPs was studied using UV-Vis and high-resolution TEM. The antibacterial activity of the particles against Erwinia amylovora was evaluated using the Kirby–Bauer disk diffusion method. The results were analyzed using ANOVA and Tukey's test (p ≤ 0.05). Both the leaf and petal extracts produced AgNPs, but the leaf extract (1 mL) was long-lasting and quasi-spherical (17.64 ± 8.87 nm), with an absorbance of UV-Vis λ
max 433 and a crystalline structure (fcc, 111). Phenols, flavonoids, tannins, and terpenoids which are associated with -OH, C=O, and C=C were identified in the extracts and could act as reducing and stabilizing agents. The best antibacterial activity was obtained with a nanoparticle concentration of 50 mg AgNPs L−1 . The main contribution of the present research is to present a sustainable method for producing nanoparticles which are stable for 2 years and with antibacterial activity against E. amylovora, one of most threatening pathogens to pear and apple productions. [ABSTRACT FROM AUTHOR]- Published
- 2024
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