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Chilli anthracnose is a major infectious disease of the genus Capsicum. Chemical control is the primary means of controlling this disease; however, the excessive use of chemical pesticides can adversely affect ecological security and human health. Here, our aim was to explore the synergistic effects of chemical and biological pesticides in the control of chilli anthracnose. The bacterial strain LY7, which is antagonistic to the anthracnose-causing fungus Colletotrichum scovillei, inhibited the growth of C. scovillei by 83.52%. Through morphological and genetic analyses, this strain was identified as Bacillus velezensis. Then, the compatibility of LY7 with three common chemical fungicides was determined. The in vitro protective and therapeutic efficacies of the 1 × 109 CFU/mL (colony-forming unit/mL) bacterial solution were 66.38% and 35.18%, respectively, but both were significantly lower than those of prochloraz, the most compatible fungicide. We then conducted field efficacy trials to elucidate the best combination of prochloraz and LY7; the highest control efficiency was achieved with a suspension of 1.0 × 108 CFU/mL of LY7 mixed with 0.75 g/L prochloraz (3:7 ratio). Electron microscopy revealed the inhibitory effects of LY7 and prochloraz on C. scovillei mycelial growth. These results suggest that an LY7-based biofungicide can partially replace prochloraz, serving as an integrated management strategy to control chilli anthracnose. [ABSTRACT FROM AUTHOR]

Almond expeller is an undeveloped reservoir of bioactive peptides. In the current study, a zinc ion ligand Arg-Pro-Pro-Ser-Glu-Asp-Glu-Asp-Gln-Glu (RPPSEDEDQE) offering a noncompetitive inhibitory effect on ACE (IC50: 205.50 μmol·L−1) was identified from almond albumin hydrolysates via papain and thermolysin hydrolysis, subsequent chromatographic separation, and UPLC-Q-TOF-MS/MS analysis. Molecular docking simulated the binding modes of RPPSEDEDQE to ACE and showed the formation of hydrogen bonds between RPPSEDEDQE and seven active residues of ACE. Moreover, RPPSEDEDQE could bind to fifteen active sites of ACE by hydrophobic interactions, and link with the His387 and zinc ions of the zinc tetrahedral coordination. Ultraviolet wavelength scanning and Fourier-transformed infrared spectroscopy analysis revealed that RPPSEDEDQE can provide multiple binding sites for zinc ions. However, RPPSEDEDQE cannot bind with any central pocket of ACE, which was evidenced by an inhibition kinetics experiment. Additionally, the zinc-chelating capacity and inhibiting ability against ACE of RPPSEDEDQE were both not significantly reduced by the hydrolysis of gastrointestinal enzymes. A moderate to high dose of RPPSEDEDQE (100–150 mg·kg bw−1) significantly reduced the systolic and diastolic blood pressure of spontaneous hypertensive rats, but chelation with zinc ions decreased its antihypertensive efficiency. These results indicate that bitter almond albumin peptides may be used for lowering blood pressure. [ABSTRACT FROM AUTHOR]

Composite bipolar plates composed of conductivity fillers and resins are widely employed in stationary proton exchange membrane fuel cell (PEMFC) due to their high corrosion resistance in acidic environments. Phenol-formaldehyde resin is a commonly used adhesive in composite bipolar plates; while numerous benzene rings in phenol-formaldehyde resin result in the poor flexibility of composite bipolar plates. Embedding the flexible segment (–CHOH–CH 2 -O-) in the resin network structure of phenol-formaldehyde resin by copolymerization with epoxy resin is an effective method for improving the defects of phenol-formaldehyde resin. In this contribution, to tailor flexible-segment-rich (–CHOH–CH 2 -O-) resin network structure, the co-polymerization mechanism of phenol-formaldehyde resin and epoxy resin is investigated in detailed by in-situ Fourier Transform Infrared (FTIR), based on which an Alter-T strategy is proposed for flexible-segment-rich (–CHOH–CH 2 -O-) resin network structure during composite bipolar plates fabrication process. The obtained composite bipolar plates consisted of graphite and flexible-segment-rich resin network structure delivers improved flexural strength (86.97 MPa), electrical conductivity (203.22 S cm−1) and areal resistance (3.41 mΩ cm2) in comparison to previously reported composite bipolar plates with same components, which are also over the technical standard of the DOE. It is further demonstrated that enriching the flexible segment for improving the poor flexibility of resin network structure is of great importance to enhance the performance of the composite bipolar plates. A two-step alteration of temperature (Alter-T) strategy was proposed to tailor flexible-segment-rich (–CHOH–CH 2 -O-) in the resin network structure during polymerization, for improving the performance of CBP including mechanical properties and electrical conductivity. This approach provides a new insight of improving CBP performance by regulating resin network structure. [Display omitted] • Epoxy is embedded in the resin network structure of Phenol-Formaldehyde. • The resin network structure flexibility is improved by embedding flexible-segment. • The composite bipolar plates exhibit improved comprehensive performance. [ABSTRACT FROM AUTHOR]

Increasing amounts of tailings and waste rubber tires have caused threats to the environment. In order to reuse these solid wastes, this study proposed a cemented tailings material containing crumb rubber recovered from waste tires. Unconfined compression tests and thermal conductivity measurements were conducted to evaluate the feasibility and performance of the proposed cemented tailings. Results revealed that the stress-strain behavior of the cemented tailings changed by the inclusion of crumb rubber. The unconfined compressive strength of cemented tailings decreased with increasing crumb rubber content. The inclusion of crumb rubber increased the failure strain and nonlinearity index of the cemented tailings. It was found that the addition of 4% crumb rubber provided the greatest improvement in ductility. The increase of the rubber content further increased the thermal insulation capacity of the cemented tailings. The low stiffness and the dimpled surface of the crumb rubber were responsible for the reduced compressive strength and thermal conductivity of the cemented tailings. In conclusion, the crumb rubber had both positive and negative effects on the cemented tailings. In practical application, the rubber admixture amount should be flexibly adjusted for different use case to make the cemented tailings material show the most suitable performance characteristic. [ABSTRACT FROM AUTHOR]

Sugarcane (Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing considerable economic losses. Transgenic technology is an effective breeding approach to improve sugarcane tolerance to drought using drought-inducible promoter(s) to activate drought-resistance gene(s). In this study, six different promoters were cloned from sugarcane bacilliform virus (SCBV) genotypes exhibiting high genetic diversity. In β-glucuronidase (GUS) assays, expression of one of these promoters (PSCBV-YZ2060) is similar to the one driven by the CaMV 35S promoter and >90% higher compared to the other cloned promoters and Ubi1. Three SCBV promoters (PSCBV-YZ2060, PSCBV-TX, and PSCBV-CHN2) function as drought-induced promoters in transgenic Arabidopsis plants. In Arabidopsis, GUS activity driven by promoter PSCBV-YZ2060 is also upregulated by abscisic acid (ABA) and is 2.2–5.5-fold higher when compared to the same activity of two plant native promoters (PScRD29A from sugarcane and PAtRD29A from Arabidopsis). Mutation analysis revealed that a putative promoter region 1 (PPR1) and two ABA response elements (ABREs) are required in promoter PSCBV-YZ2060 to confer drought stress response and ABA induction. Yeast one-hybrid and electrophoretic mobility shift assays uncovered that transcription factors ScbZIP72 from sugarcane and AREB1 from Arabidopsis bind with two ABREs of promoter PSCBV-YZ2060. After ABA treatment or drought stress, the expression levels of endogenous ScbZIP72 and heterologous GUS are significantly increased in PSCBV-YZ2060:GUS transgenic sugarcane plants. Consequently, promoter PSCBV-YZ2060 is a possible alternative promoter for genetic engineering of drought-resistant transgenic crops such as sugarcane. The promoter PSCBV-YZ2060 cloned from the sugarcane bacilliform virus is shown to be induced by drought in sugarcane and Arabidopsis plants and is a potential alternative promoter for genetic engineering of drought-resistant transgenic crops. [ABSTRACT FROM AUTHOR]

Composite bipolar plates (CBP) composed of conductive carbon and flexible but insulated resin are considered promising skeletons in proton exchange membrane fuel cell (PEMFC) with the characteristics of desirable mechanical and electrical conductivity properties, as well as chemical stability. However, the paradox of mechanical and electrical properties caused by insulating resins limits the overall performance of the CBP. Recently, massive studies have been reported to develop high-performance practical CBP; while there is few detailed summarizations yet. This review covers the recent advances in materials and technologies of high-performance CBP comprehensively. First, the core materials supporting the electrical conductivity and mechanical properties are summarized in detail. Furthermore, the interaction between resins and carbon-based materials is analyzed carefully. Subsequently, the fabrication process and novel structure of CBP are reviewed. Finally, the existing challenges are also discussed, and several future research directions are proposed. Overall, this review can provide guidelines for selecting appropriate strategies for improved CBP in PEMFC. • The materials and technologies of composite bipolar plate are reviewed. • The core materials for electrical and mechanical properties are summarized. • The fabrication process and novel structure of CBP are reviewed. • Suggestions for future development of composite bipolar plate are discussed. [ABSTRACT FROM AUTHOR]

Background: The university years are a developmentally crucial phase and a peak period for the onset of mental disorders. The beliefs about the changeability of negative emotion may play an important role in help-seeking. The brief digital growth mindset intervention is potentially scalable and acceptable to enhance adaptive coping and help-seeking for mental health needs in university students. We adapted the Single-session Intervention on Growth Mindset for adolescents (SIGMA) to be applied in university students (U-SIGMA). This protocol introduces a two-armed waitlist randomized controlled trial study to examine the effectiveness and acceptability of U-SIGMA in promoting help-seeking among university students in the Greater Bay Area. Methods: University students (N = 250, ages 18–25) from universities in the Greater Bay Area will be randomized to either the brief digital growth mindset intervention group or the waitlist control group. Participants will report on the mindsets of negative emotions, perceived control over anxiety, attitude toward help-seeking, physical activity, hopelessness, psychological well-being, depression, anxiety, and perceived stress at baseline and the 2-week and 8-week follow-ups through web-based surveys. A 30-min digital intervention will be implemented in the intervention group, with a pre- and post-intervention survey collecting intervention feedback, while the control group will receive the link for intervention after 8 weeks. Discussion: This protocol introduces the implementation plan of U-SIMGA in multi-cities of the Greater Bay Area. The findings are expected to help provide pioneer evidence for the effectiveness and acceptability of the brief digital intervention for university students in the Chinese context and beyond and contribute to the development of accessible and effective prevention and early intervention for university students' mental health. Trial registration: HKU Clinical Trials Registry: HKUCTR-3012; Registered 14 April 2023. http://www.hkuctr.com/Study/Show/7a3ffbc0e03f4d1eac0525450fc5187e. [ABSTRACT FROM AUTHOR]

Choanephora rot of Chenopodium plants (CRC) was observed at the flowering stages in seven plantations of Shanxi Province, China. CRC had caused leaf, stem, and panicle neck rot of C. quinoa, panicle neck and stem rot of C. formosanum, and stem rot of C. album. Typical symptoms included water-soaked, rapid soft rotting, and abundant sporulation on the whole panicle necks, stems, and leaves. Based on morphological characteristics, phylogenetic analyses, and pathogenicity tests, the pathogens were identified as Choanephoraceae cucurbitarum. Sporangiola and sporangiospore of C. cucurbitarum germinated at 30 °C and were able to germinate by two h post-inoculation (hpi). The germination rates of sporangiola and sporangiospore significantly increased at 3 to 4 hpi, and the germination rates ranged from 91.53 to 97.67%. The temperature had a significant effect on the pathogenicity of C. cucurbitarum the optimum pathogenic temperatures for stems of C. quinoa, C. formosanum and C. album were 30 °C after one day post-inoculation. Choanephoraceae cucurbitarum could infect white and red quinoa panicle necks between 20 and 30 °C, and the average lesion lengths were 0.21 to 3.62 cm. Among the five tested fungicides (boscalid, dimethomorph, isopyrazam, propiconazole, and tebuconazole), isopyrazam showed higher sensitivity to sporangiola germination of C. cucurbitarum, with an EC50 value of 0.6550 μg/mL. Isopyrazam and tebuconazole strongly inhibited the sporangiospore germination of C. cucurbitarum, which showed EC50 values of 0.4406 and 0.3857 μg/mL. To our knowledge, the present study found for the first time that C. cucurbitarum is a pathogen causing panicle neck of C. formosanum and stem rot of C. formosanum and C. album, while CRC first appeared in the quinoa panicle necks, and gradually expanded to stems and leaves. [ABSTRACT FROM AUTHOR]

Background: Colorectal cancer (CRC), a commonly diagnosed cancer often develops slowly from benign polyps called adenoma to carcinoma. Altered gut microbiota is implicated in colorectal carcinogenesis. It is warranted to find non-invasive progressive microbiota biomarkers that can reflect the dynamic changes of the disease. This study aimed to identify and evaluate potential progressive fecal microbiota gene markers for diagnosing advanced adenoma (AA) and CRC. Results: Metagenome-wide association was performed on fecal samples from different cohorts of 871 subjects (247 CRC, 234 AA, and 390 controls). We characterized the gut microbiome, identified microbiota markers, and further constructed a colorectal neoplasms classifier in 99 CRC, 94 AA, and 62 controls, and validated the results in 185 CRC, 140 AA, and 291 controls from 3 independent cohorts. 21 species and 277 gene markers were identified whose abundance was significantly increased or decreased from normal to AA and CRC. The progressive gene markers were distributed in metabolic pathways including amino acid and sulfur metabolism. A diagnosis model consisting of four effect indexes was constructed based on the markers, the sensitivities of the Adenoma Effect Index 1 for AA, Adenoma Effect Index 2 for high-grade dysplasia (HGD) adenoma were 71.3% and 76.5%, the specificities were 90.5% and 90.3%, respectively. CRC Effect Index 1 for all stages of CRC and CRC Effect Index 2 for stage III–IV CRC to predict CRC yielded an area under the curve (AUC) of 0.839 (95% CI 0.804–0.873) and 0.857 (95% CI 0.793–0.921), respectively. Combining with fecal immunochemical test (FIT) significantly improved the sensitivity of CRC Effect Index 1 and CRC Effect Index 2 to 96.7% and 100%. Conclusions: This study reports the successful diagnosis model establishment and cross-region validation for colorectal advanced adenoma and carcinoma based on the progressive gut microbiota gene markers. The results suggested that the novel diagnosis model can significantly improve the diagnostic performance for advanced adenoma. [ABSTRACT FROM AUTHOR]

Background: Fear of cancer recurrence (FCR) has been demonstrated to be one of the most frequently reported unmet psychological needs among cancer survivors. The aim of this study was to explore and describe the potential triggers and coping strategies for FCR in Chinese cancer survivors. Methods: The study process was conducted using an interpretive phenomenological research method, and Chinese cancer survivors were interviewed face-to-face in a semi-structured interview, using purposive sampling combined with a maximum variance sampling strategy, and the interviews were transcribed, organized, and analyzed by applying Giorgi analysis with the help of NVivo11 software. Results: A total of 10 participants, 4 males and 6 females, were interviewed. Three themes emerged in terms of potential triggers for FCR: (1) intrusive thoughts; (2) disease symptoms; and (3) awaiting medical examination. Two themes regarding positive coping and avoidance coping emerged with regard to coping strategies adopted by cancer survivors when experiencing FCR. Under these 2 themes were 5 sub-themes: (1) seeking medical support; (2) self-health management; (3) spiritual coping; (4) unaccompanied toleration; and (5) attention shifting. Conclusion: FCR as the most common psychological problem for cancer survivors, and it should be given more attention. Early identification and precise intervention for potential triggers of FCR may prevent the emergence and development of FCR. The guidance toward and cultivation of positive coping strategies when cancer survivors experience FCR could be an important direction in future nursing education. [ABSTRACT FROM AUTHOR]

Metastasis is an important cause of cancer-related death. Previous studies in our laboratory found that pregnane alkaloids from Pachysandra terminalis had antimetastatic activity against breast cancer cells. In the current study, we demonstrated that treatment with one of the alkaloid derivatives, (Z)-3β-ethylamino-pregn-17(20)-en (1), led to the downregulation of the HIF-1α/VEGF/VEGFR2 pathway, suppressed the phosphorylation of downstream molecules Akt, mTOR, FAK, and inhibited breast cancer metastasis and angiogenesis both in vitro and in vivo. Furthermore, the antimetastasis and antiangiogenesis effects of 1 treatment (40 mg/kg) were more effective than that of Sorafenib (50 mg/kg). Surface plasmon resonance (SPR) analysis was performed and the result suggested that HSP90α was a direct target of 1. Taken together, our results suggested that compound 1 might represent a candidate antitumor agent for metastatic breast cancer. [ABSTRACT FROM AUTHOR]

Deep resource extraction has been affected by the complex geological environment of "three highs and one disturbance" for a long time. The surrounding rocks experience strong unloading stress disturbance during the underground resource extraction. The creep characteristics of the perimeter rocks are of great theoretical and practical value. Firstly, the triaxial pre-peak loading and unloading tests on the prepared samples are conducted on the intact rock samples using the TAW-200 rock mechanics test system. Then the rock samples with different degrees of pre-peak unloading damage were prepared under the condition of pre-peak yield. Finally, the uniaxial creep tests were carried out to study the uniaxial creep mechanical properties. The results show that the wave velocities of the damaged rock samples are reduced to different degrees compared with those of the intact rock samples, the creep of the pre-peak unloading damaged rock has a time-dependent damage effect, and the study results are similar to those of the conventional uniaxial creep test of the white sandstone. The instantaneous strain at all stress levels of the damaged rock samples increased gradually with the stress level, which is consistent with the non-linear functional relationship; Based on the time-dependent damage effect, the two-parameter Weibull distribution function was introduced into the West Plains viscoelasticity model, and a creep damage model for white sandstone was proposed. The improved Nishihara model can simulate the uniaxial creep characteristics of each damaged rock sample at various stress levels. [ABSTRACT FROM AUTHOR]

• The pepper endophyte Bacillus velezensis LY7 has antifungal activity. • Strain LY7 contains a rare cupin domain-containing protein OS with antifungal activity. • Cupin domain-containing protein OS induces the expression of genes in anthracnose. • LY7 increased JA and SA levels to improve the stress resistance of pepper plants. • LY7 treatment to seedlings has potential for pepper biocontrol and growth promotion. Anthracnose of pepper (Capsicum annuum L), caused by Colletotrichum scovillei , is one of the three major diseases of pepper that seriously impact yield and quality. As an alternative to the use of synthetic chemical pesticides, a variety of endophytic bacteria have been reported to be potential biocontrol agents with the ability to improve disease resistance and abiotic stress tolerance in host plants. However, the mechanisms of action by which these different bacterial biocontrol agents inhibit the onset and development of disease, in some cases, remains unclear. In the present study, the potential use of the endophytic bacterial strain Bacillus velezensis LY7, originally isolated from pepper leaves, as a biocontrol agent was evaluated. More specifically, the ability of LY7 to control anthracnose in pepper and promote growth was assessed in pot experiments in a greenhouse and in vitro assays. Results indicated that the use of B. velezensis LY7 enhanced the synthesis of jasmonic acid and other hormones in pepper plants, as well as increased disease resistance and plant growth. A 200 × dilution of LY7 fermentation broth reduced disease incidence up to 90.70 %, while also enhancing plant growth. The mechanism of action of LY7 was also evaluated. Results indicated that LY7 secreted an antibiotic protein possessing a cupin domain OS. RNA-sequence analysis of the transcriptome of C. scovillei at several timepoints after exposure to LY7 (24, 48, and 72 h) revealed the up- and down-regulation of several genes. More specifically, LY7 induced the expression of genes associated with biosynthesis pathways and transcription factors involved in anthracnose pathogenicity. Our results indicate that the LY7 strain of induces hormone synthesis in pepper plants that results in enhanced disease resistance and promotes growth, as well as enhances stress tolerance by enhancing antioxidant and defense-related enzyme activity. These results demonstrate the potential of using LY7 for the biocontrol of anthracnose in pepper plants. [ABSTRACT FROM AUTHOR]

Simple Summary: The malignant growth and therapy resistance of isocitrate dehydrogenase (IDH)-wildtype glioblastoma is thought to be driven by a subpopulation of tumor cells with cancer stem-like cell (CSC) properties. Employing a high-throughput in vitro drug screen, we identified LGK974 and berberine as drugs that impair wingless (WNT) signaling and can thereby sensitize glioblastoma stem-like cells (GSCs) to glucose starvation-induced cell death. The main goal of this study was to characterize the role of the WNT pathway in mediating the survival and metabolic plasticity of GSCs under nutrient-restricted growth conditions. Gas chromatography mass spectrometry (GC-MS) was used to determine WNT-specific alterations of intracellular metabolites in GSCs grown under nutrient restriction, i.e., glucose depletion, or under standard conditions. Metabolic fingerprints hold the promise to complement classic biomarkers, thus potentially aiding the prediction of tumor behavior and patient prognosis. Isocitrate dehydrogenase (IDH)-wildtype glioblastoma is the most common primary malignant brain tumor. It is associated with a particularly poor prognosis, as reflected by an overall median survival of only 15 months in patients who undergo a supramarginal surgical reduction of the tumor mass followed by combined chemoradiotherapy. The highly malignant nature of IDH-wildtype glioblastoma is thought to be driven by glioblastoma stem-like cells (GSCs) that harbor the ability of self-renewal, survival, and adaptability to challenging environmental conditions. The wingless (WNT) signaling pathway is a phylogenetically highly conserved stemness pathway, which promotes metabolic plasticity and adaptation to a nutrient-limited tumor microenvironment. To unravel the reciprocal regulation of the WNT pathway and the nutrient-limited microenvironment, glioblastoma cancer stem-like cells were cultured in a medium with either standard or reduced glucose concentrations for various time points (24, 48, and 72 h). Glucose depletion reduced cell viability and facilitated the survival of a small population of starvation-resistant tumor cells. The surviving cells demonstrated increased clonogenic and invasive properties as well as enhanced chemosensitivity to pharmacological inhibitors of the WNT pathway (LGK974, berberine). Glucose depletion partially led to the upregulation of WNT target genes such as CTNNB1, ZEB1, and AXIN2 at the mRNA and corresponding protein levels. LGK974 treatment alone or in combination with glucose depletion also altered the metabolite concentration in intracellular compartments, suggesting WNT-mediated metabolic regulation. Taken together, our findings suggest that WNT-mediated metabolic plasticity modulates the survival of GSCs under nutrient-restricted environmental conditions. [ABSTRACT FROM AUTHOR]

Deep high-stress roadway excavation under unloading disturbance inevitably leads to damage deterioration of the surrounding rock, which poses a serious threat to its stability. To explore the energy characteristics of white sandstone damaged by peak front unloading, uniaxial compression tests were conducted on damaged rock samples. The results show that the peak strength and modulus of elasticity of the rock sample gradually decrease with increasing damage degrees. The external work input energy, releasable elastic strain energy and dissipation energy all decreased with increasing damage. Damage evolution curves and equations of the rock samples were obtained based on the damage instantiation model established by the principle of energy dissipation and release. The effects of unloading damage on the fracture characteristics of the rock samples were analysed from both macro and microscopic viewpoints, and the results showed that a micro fracture in the rock is transformed from brittle–ductile damage, while macroscopic damage occurs in the form of a "shear"-"splitting"-"mixed shear-splitting" damage process. This paper has certain research and reference value for understanding the damage evolution characteristics of rocks with peak front unloading damage. [ABSTRACT FROM AUTHOR]

Progress toward intelligent human–robotic interactions requires monitoring sensors that are mechanically flexible, facile to implement, and able to harness recognition capability under harsh environments. Conventional sensing methods have been divided for human‐side collection or robot‐side feedback and are not designed with these criteria in mind. However, the iontronic polymer is an example of a general method that operates properly on both human skin (commonly known as skin electronics or iontronics) and the machine/robotic surface. Here, a unique iontronic composite (silk protein/glycerol/Ca(II) ion) and supportive molecular mechanism are developed to simultaneously achieve high conductivity (around 6 kΩ at 50 kHz), self‐healing (within minutes), strong stretchability (around 1000%), high strain sensitivity and transparency, and universal adhesiveness across a broad working temperature range (−40–120 °C). Those merits facilitate the development of iontronic sensing and the implementation of damage‐resilient robotic manipulation. Combined with a machine learning algorithm and specified data collection methods, the system is able to classify 1024 types of human and robot hand gestures under challenging scenarios and to offer excellent object recognition with an accuracy of 99.7%. [ABSTRACT FROM AUTHOR]

Objectives: Immunoglobulin A vasculitis (IgAV), previously known as Henoch–Schönlein purpura, is the most common vasculitis that has a classical skin manifestation of palpable purpuric rash. Factors pertinent to IgAV remain inadequately understood. Here, we aimed to examine the gut microbiome shifts associated with IgAV and its recovery. Methods: Stool samples were collected from 10 children with IgAV (6–14 years old) before and after a multi‐drug therapy, along with 9 age‐matched healthy children. The samples were subjected to metagenomic analyses to investigate the taxonomic and functional shifts of the gut microbiome. Results: The analyses revealed that compared with healthy controls, treatment‐naïve patients exhibited substantial taxonomic and functional alterations of gut microbiota, including 104 IgAV‐depleted species and 7 IgAV‐elevated species (FDR < 0.05). After treatment, the IgAV patients displayed a partial restoration of the microbiota shifts, as the relative abundances of some biomarkers (e.g. 9 genera and 22 species) became comparable (FDR > 0.1) between the patients and healthy controls. The treatment‐responsive features included Weissella, Faecalibacterium prausnitzii and Bifidobacterium pseudocatenulatum and three components of a putative glutamine transport system. Importantly, gram‐positive bacteria accounted for over 85% of the numbers and total relative abundance of the species that were associated with IgAV and responsive to the treatment. In addition, of the 122 IgAV‐depleted bacterial genes, 82 were mainly contributed by gram‐positive bacteria and 12 by gram‐negative bacteria. Conclusions: Gram‐positive bacteria are the main drivers underlying the gut microbiome shifts of IgAV, which may assist rational management of the disease. [ABSTRACT FROM AUTHOR]

Composite bipolar plates (CBPs) are promising candidates in proton exchange membrane fuel cell (PEMFC) for achieving mechanical strength and electrical conductivity as well as chemical stability in acidic environments simultaneously. While widely-explored CBPs composed with Phenol-Formaldehyde (PF) resin as adhesive (donated as PF-CBP) deliver poor flexibility due to the numerous hard π-conjugation (benzene ring) in PF network structure, which is becoming the main bottleneck index and largely hindering the practical applications of CBP. Herein, we propose a co-polymerization method by introducing Epoxy (EP) into PF resin as adhesive for CBP (donated as PF-EP-CBP), by which abundant flexible segments (-CHOH-CH 2 -O-) from EP are demonstrated to be well embedded into the structure of PF resin for enhanced flexibility. The obtained PF-EP-CBP exhibits significantly improved flexural strength of 46.20 MPa, 1.57 times higher than pristine PF-CBP (29.58 MPa), which is also higher than the 2025 DOE targets (40 MPa). Moreover, the detailed areal specific resistance, thermal conductivity, H 2 permeability and corrosion behaviors are conducted systematically to support the single cell of PEMFC. The results provide a novel insight and convenient method on the adhesive tailoring by incorporating flexible linkers into resin network structure, which is of significance to drive the practical development of the CBPs in PEMFC fields. [Display omitted] • Epoxy is embedded in the resin network structure of Phenol-Formaldehyde. • The flexibility of resin network structure could be improved by embedding Epoxy. • The composite bipolar plates exhibit improved flexural strength of 46.20 MPa. [ABSTRACT FROM AUTHOR]

B Silk-Based Iontronics b In article number 2102596, Tiger H. Tao and co-workers report a unique silk-based iontronic composite to simultaneously achieve high conductivity, self-healing, strong stretchability, high strain sensitivity and transparency, and universal adhesiveness across a broad working temperature range. Robotic Manipulation under Harsh Conditions Using Self-Healing Silk-Based Iontronics (Adv. Sci. 2/2022) Those merits facilitate the implementation of damage-resilient intelligent robotic manipulation. [Extracted from the article]

[Display omitted] • Chiral ionone alkaloid ION-31a inhibits breast cancer metastasis and angiogenesis. • Its direct target was identified as HSP90α. • ION-31a downregulates HSP90/HIF-1α/VEGF/VEGFR2 pathway. Breast cancer has become the number one killer of women. In our previous study, an active compound, ION-31a, with potential anti-metastasis activity against breast cancer was identified through the synthesis of ionone alkaloid derivatives. In the present study, we aimed to identify the therapeutic target of ION-31a. We used a fluorescence tag labeled probe, molecular docking simulation, and surface plasmon resonance (SPR) analysis to identify the target of ION-31a. The main target of ION-31a was identified as heat shock protein 90 (HSP90). Thus, ION-31a is a novel HSP90 inhibiter that could suppress the metastasis of breast cancer and angiogenesis significantly in vitro and in vivo. ION-31a acts via inhibiting the HSP90/hypoxia inducible factor 1 alpha (HIF-1α)/vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) pathway and downregulating downstream signal pathways, including protein kinase B (AKT)/mammalian target of rapamycin (mTOR), AKT2/protein kinase C epsilon (PKCζ), extracellular regulated kinase 1/2 (ERK1/2), focal adhesion kinase (FAK), and mitogen-activated protein kinase 14 (p38MAPK) pathways. ION-31a affects multiple effectors implicated in tumor metastasis and has the potential to be developed as an anti-metastatic agent to treat patients with breast cancer. [ABSTRACT FROM AUTHOR]