22 results on '"Wu, Yi-Ru"'
Search Results
2. MicroRNA‐299‐3p inhibits cell proliferation, motility, invasion and angiogenesis via VEGFA in upper tract urothelial carcinoma.
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Wang, Chien‐Shen, Lee, Yi‐Chen, Jhan, Jhen‐Hao, Li, Wei‐Ming, Chang, Lin‐Li, Huang, A‐Mei, Lin, Hui‐Hui, Wu, Yi‐Ru, Hsu, Wei‐Chi, and Ke, Hung‐Lung
- Abstract
Background: Upper tract urothelial carcinoma (UTUC) is a rare tumor with extraordinarily different features between Eastern and Western countries. Vascular endothelial growth factor‐A (VEGFA) was originally identified as a secreted signaling protein and regulator of vascular development and cancer progression. In this study, we aimed to elucidate the molecular mechanisms underlying the regulation of VEGFA by microRNA in UTUC. Methods: VEGFA expression was evaluated by immunohistochemistry in 140 human UTUC tissue samples. Next, we assessed the regulatory relationship between VEGFA and miR‐299‐3p by real‐time PCR, western blotting, ELISA and dual‐luciferase reporter assays using two UTUC cell lines. The role of miR‐299‐3p/VEGFA in cell proliferation, motility, invasion, and tube formation was analyzed in vitro. Results: High VEGFA expression was significantly associated with tumor stage, grade, distant metastasis and cancer‐related death and correlated with poor progression‐free and cancer‐specific survival. VEGFA knockdown repressed proliferation, migration, invasion and angiogenesis in UTUC cell lines. miR‐299‐3p significantly reduced VEGFA protein expression and miR‐299‐3p overexpression inhibited VEGFA mRNA and protein expression by directly targeting its 3′‐UTR. Functional studies indicated that VEGFA overexpression reversed the miR‐299‐3p‐mediated suppression of tumor cell proliferation, migration, invasion and angiogenesis. In addition, miR‐299‐3p/VEGFA suppressed cellular functions in UTUC by modulating the expression of P18 and cyclin E2. Conclusions: Our findings suggest that miR‐299‐3p possibly suppresses UTUC cell proliferation, motility, invasion and angiogenesis via VEGFA. VEGFA may act as a prognostic predictor, and both VEGFA and miR‐299‐3p could be potential therapeutic targets for UTUC. [ABSTRACT FROM AUTHOR]
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- 2024
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3. MicroRNA‐145‐5p suppresses cell proliferation, migration, and invasion in upper tract urothelial carcinoma by targeting 5‐aminoimidazole‐4‐carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.
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Luo, Hao‐Lun, Lee, Yi‐Chen, Chang, Yin‐Lun, Hsu, Wei‐Chi, Wu, Yen‐Ting, Jhan, Jhen‐Hao, Lin, Hui‐Hui, Wu, Yi‐Ru, Ke, Hung‐Lung, and Liu, Hui‐Ying
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- 2023
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4. Shape Dependence of Silver-Nanoparticle-Mediated Synthesis of Gold Nanoclusters with Small Molecules as Capping Ligands.
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Chang, Cheng-Yeh, Wu, Yi-Ru, Tseng, Tzu-Hsien, Su, Jun-Hao, Wang, Yu-Shan, Jen, Fang-Yi, Chen, Bo-Ru, Huang, Cheng-Liang, and Chen, Jui-Chang
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SMALL capitalization stocks , *SMALL molecules , *SILVER nanoparticles , *LIGANDS (Chemistry) , *GOLD clusters , *TRANSMISSION electron microscopy - Abstract
In this study, differently shaped silver nanoparticles used for the synthesis of gold nanoclusters with small capping ligands were demonstrated. Silver nanoparticles provide a reaction platform that plays dual roles in the formation of Au NCs. One is to reduce gold ions and the other is to attract capping ligands to the surface of nanoparticles. The binding of capping ligands to the AgNP surface creates a restricted space on the surface while gold ions are being reduced by the particles. Four different shapes of AgNPs were prepared and used to examine whether or not this approach is dependent on the morphology of AgNPs. Quasi-spherical AgNPs and silver nanoplates showed excellent results when they were used to synthesize Au NCs. Spherical AgNPs and triangular nanoplates exhibited limited synthesis of Au NCs. TEM images demonstrated that Au NCs were transiently assembled on the surface of silver nanoparticles in the method. The formation of Au NCs was observed on the whole surface of the QS-AgNPs if the synthesis of Au NCs was mediated by QS-AgNPs. In contrast, formation of Au NCs was only observed on the edges and corners of AgNPts if the synthesis of Au NCs was mediated by AgNPts. All of the synthesized Au NCs emitted bright red fluorescence under UV-box irradiation. The synthesized Au NCs displayed similar fluorescent properties, including quantum yields and excitation and emission wavelengths. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. High Transaldolase 1 expression predicts poor survival of patients with upper tract urothelial carcinoma.
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Wu, Yi‐Ru, Lee, Yi‐Chen, Li, Wei‐Ming, Hsu, Wei‐Chi, Lin, Hui‐Hui, Chang, Lin‐Li, Huang, A‐Mei, Jhan, Jhen‐Hao, Wu, Wen‐Jeng, Li, Ching‐Chia, Lee, Hsiang‐Ying, Yeh, Hsin‐Chih, and Ke, Hung‐Lung
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TRANSITIONAL cell carcinoma , *PENTOSE phosphate pathway , *PROGNOSIS , *PROGRESSION-free survival ,WESTERN countries - Abstract
Upper tract urothelial carcinoma (UTUC) is a rare tumor with an incidence that varies greatly between Eastern and Western countries. Transaldolase 1 (TALDO1) is a rate‐limiting enzyme of the pentose phosphate pathway. In humans, aberrant TALDO1 activity has been implicated in various autoimmune diseases and malignancies; however, the function of TALDO1 in UTUC has not been previously investigated. Here we evaluated the clinical significance of TALDO1 expression in 115 paraffin‐embedded tumor samples from patients with UTUC using immunohistochemistry. Our results demonstrated that there was an association between high TALDO1 expression and advanced stage (P = 0.011), tumor size (P = 0.005), tumor location (P = 0.047), distant metastases (P = 0.023), local recurrence (P = 0.002), and cancer death (P = 0.003). Using univariate and multivariate analyses, we found that chemotherapy was an independent factor for bladder recurrence‐free survival. Late stage (III/IV) and high TALDO1 expression were independent prognostic factors for progression‐free and cancer‐specific survival. In summary, increased TALDO1 expression in UTUC was significantly correlated with late stage, tumor size, tumor location, distant metastases, local recurrence, and cancer death. Therefore, high TALDO1 expression could be a predictor of poor survival in patients with UTUC. Further studies are necessary to investigate the role of TALDO1 in UTUC development. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Laminarin modulates the chloroplast antioxidant system to enhance abiotic stress tolerance partially through the regulation of the defensin-like gene expression.
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Wu, Yi-Ru, Lin, Yi-Chen, and Chuang, Huey-wen
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ARABIDOPSIS , *POLYSACCHARIDES , *EFFECT of salt on plants , *CHLOROPLASTS , *PLANT genetics , *ABIOTIC stress , *GENE expression in plants - Abstract
Algae wall polysaccharide, laminarin (Lam), has an established role on induction of plant disease resistance. In this study, application of Lam increased Arabidopsis fresh weight and enhanced tolerance to salt and heat stress by stabilizing chloroplast under adverse environment. Transcriptome analysis indicated that, in addition to induced a large number of genes associated with the host defense, genes involved in the regulation of abiotic stress tolerance mostly the heat stress response constituted the largest group of the up-regulated genes. Lam induced expression of IRT1 , ZIP8 , and copper transporters involved in transport of Fe, Zn, Cu ions associated with the activity of chloroplast antioxidant system. Lam also up-regulated genes involved in the synthesis of terpenoid, a plastidial-derived secondary metabolite with antioxidant activity. Overexpression of a Lam-induced defensin like 202 ( DEFL202 ) resulted in increased chloroplast stability under salt stress and increased plant growth activity after heat stress. Expression of antioxidant enzymes including SOD and ascorbate peroxidase ( APX ), photosystem PsbA-D1 and ABA-dependent responsive to desiccation 22 ( RD22 ) was induced to higher levels in the transgenic seedlings. In sum, our results suggest that Lam is an potent inducer for induction of chloroplastic antioxidant activity. Lam affect plant abiotic stress tolerance partially through regulation of the DEFL -mediated pathway. [ABSTRACT FROM AUTHOR]
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- 2016
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7. MicroRNA-375-3p Suppresses Upper Tract Urothelial Carcinoma Cell Migration and Invasion via Targeting Derlin-1.
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Jhan, Jhen-Hao, Hsu, Wei-Chi, Lee, Yi-Chen, Li, Wei-Ming, Huang, A-Mei, Lin, Hui-Hui, Wang, Chien-Sheng, Wu, Yi-Ru, Li, Ching-Chia, Wu, Wen-Jeng, and Ke, Hung-Lung
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PROTEIN metabolism ,BLADDER tumors ,PROTEINS ,CANCER invasiveness ,IMMUNOHISTOCHEMISTRY ,MICRORNA ,MOLECULAR biology ,GENE expression ,EPITHELIAL-mesenchymal transition ,TUMOR suppressor genes ,CELL migration inhibition ,CELL proliferation ,TUMOR markers - Abstract
Simple Summary: The Derlin-1 protein, encoded by DERL1, is located in the endoplasmic reticulum membrane and is responsible for the unfolded protein response. Derlin-1 has recently drawn lots of attention with regard to cancer pathogenesis. The purpose of this study is to examine its role and molecular mechanism in upper tract urothelial carcinoma (UTUC). The protein levels of Derlin-1 in human UTUC specimens are observed by immunohistochemistry. Derlin-1 overexpression is evidently associated with cancer stage, distant metastasis, recurrence, and poor prognosis in patients with UTUC. In an in vitro study, the knockdown of Derlin-1 represses, while over-expression of Derlin-1 increases migration and invasion of UTUC cells significantly. We identify the microRNA-375-3p (miR-375-3p) as one of the potential candidate microRNAs and then examine the relationship between Derlin-1 and miR-375-3p. The dual-luciferase reporter assay confirms that miR-375-3p directly targets Derlin-1. Furthermore, we reveal that miR-375-3p modulate Derlin-1 and epithelial–mesenchymal transition (EMT) marker protein levels. However, the above effects are reversed by the restoration of Derlin-1. Our study clarifies that miR-375-3p represses invasion and migration by directly targeting Derlin-1 and regulating the expression of EMT-associated proteins in UTUC cells, suggesting Derlin-1 may act as a useful predictor of prognosis and that both Derlin-1 and miR-375-3p could be potential therapeutic targets in UTUC. Little is known regarding the molecular characterization of upper tract urothelial carcinoma (UTUC). Novel therapeutic targets and prognostic predictors are imminent. In the present study, we aim to examine the oncogenic function and molecular mechanism of Derlin-1 in UTUC. Derlin-1 overexpression is significantly associated with poor prognosis in patients with UTUC. In vitro, knockdown or over-expression of Derlin-1 markedly regulated UTUC cell invasion and migration. We further discovered miR-375-3p suppresses cell invasion and migration by inversely regulating Derlin-1 and blocking EMT in UTUC cells. Taking this together, miR-375-3p functions as a tumor suppressive microRNA by directly targeting Derlin-1 and blocking epithelial–mesenchymal transition (EMT) in UTUC. [ABSTRACT FROM AUTHOR]
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- 2022
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8. Silver Nanoparticle-Mediated Synthesis of Fluorescent Thiolated Gold Nanoclusters.
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Chang, Cheng-Yeh, Tseng, Tzu-Hsien, Chen, Bo-Ru, Wu, Yi-Ru, Huang, Cheng-Liang, and Chen, Jui-Chang
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GOLD nanoparticles ,SILVER nanoparticles ,SILVER ,REDUCING agents ,ELECTRIC fields ,LIGANDS (Chemistry) - Abstract
A new strategy using silver nanoparticles (Ag NPs) to synthesize thiolated Au NCs is demonstrated. The quasi-spherical Ag NPs serve as a platform, functioning as a reducing agent for Au (III) and attracting capping ligands to the surface of the Ag NPs. Glutathione disulfide (GSSG) and dithiothreitol (DTT) were used as capping ligands to synthesize thiolated Au NCs (glutathione-Au NCs and DTT-Au NCs). The glutathione-Au NCs and DTT-Au NCs showed red color luminance with similar emission wavelengths (630 nm) at an excitation wavelength of 354 nm. The quantum yields of the glutathione-Au NCs and DTT-Au NCs were measured to be 7.3% and 7.0%, respectively. An electrophoretic mobility assay showed that the glutathione-Au NCs moved toward the anode, while the DTT-Au NCs were not mobile under the electric field, suggesting that the total net charge of the thiolated Au NCs is determined by the charges on the capping ligands. The detection of the K
SV values, 26 M−1 and 0 M−1 , respectively, revealed that glutathione-Au NCs are much more accessible to an aqueous environment than DTT-Au NCs. [ABSTRACT FROM AUTHOR]- Published
- 2021
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9. Small Molecules Inducing Autophagic Degradation of Expanded Polyglutamine Protein through Interaction with Both Mutant ATXN3 and LC3.
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Lin, Te-Hsien, Chen, Wan-Ling, Hsu, Shao-Fan, Chen, I-Cheng, Lin, Chih-Hsin, Chang, Kuo-Hsuan, Wu, Yih-Ru, Chen, Yi-Ru, Yao, Ching-Fa, Lin, Wenwei, Lee-Chen, Guey-Jen, and Chen, Chiung-Mei
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TUBULINS ,SMALL molecules ,SPINOCEREBELLAR ataxia ,CHEMICAL derivatives ,REACTIVE oxygen species ,IMMUNOPRECIPITATION - Abstract
Polyglutamine (polyQ)-mediated spinocerebellar ataxia (SCA), including SCA1, 2, 3, 6, 7, and 17, are caused by mutant genes with expanded CAG repeats, leading to the intracellular accumulation of aggregated proteins, the production of reactive oxygen species, and cell death. Among SCA, SCA3 is caused by a mutation in the ATXN3 (ataxin-3) gene. In a circumstance of polyQ aggregation, the autophagic pathway is induced to degrade the aggregated proteins, thereby suppressing downstream deleterious effects and promoting neuronal survival. In this study, we tested the effects of synthetic indole (NC009-1, -2, -3, -6) and coumarin (LM-022, -031) derivatives as chemical chaperones to assist mutant ATXN3-Q
75 folding, as well as autophagy inducers to clear aggregated protein. Among the tested compounds, NC009-1, -2, and -6 and LM-031 interfered with Escherichia coli-derived ATXN3-Q75 aggregation in thioflavin T binding and filter trap assays. In SH-SY5Y cells expressing GFP-fused ATXN3-Q75 , these compounds displayed aggregation-inhibitory and neurite growth-promoting potentials compared to untreated cells. Furthermore, these compounds activated autophagy by increasing the phosphatidylethanolamine-conjugated LC3 (microtubule associated protein 1 light chain 3)-II:cytosolic LC3-I ratio in these cells. A biochemical co-immunoprecipitation assay by using a mixture of HEK 293T cell lysates containing recombinant ATXN3-Q75 -Venus-C-terminus (VC) or Venus-N-terminus (VN)-LC3 protein indicated that NC009-1 and -2 and LM-031 served as an autophagosome-tethering compound (ATTEC) to interact with ATXN3-Q75 and LC3, and the interaction was further confirmed by bimolecular fluorescence complementation analysis in cells co-expressing both ATXN3-Q75 -VC and VN-LC3 proteins. The study results suggest the potential of NC009-1 and -2 and LM-031 as an ATTEC in treating SCA3 and, probably, other polyQ diseases. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. Applying a Watershed and Reservoir Model in an Off-Site Reservoir to Establish an Effective Watershed Management Plan.
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Chen, Chi-Feng, Wu, Yi-Ru, and Lin, Jen-Yang
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WATERSHED management ,WATER pollution ,RESERVOIRS ,WATERSHEDS ,WATER quality ,SEWAGE - Abstract
Off-site reservoirs use water from other watersheds to supplement their water quantity. Water quality is usually better in off-site reservoirs than in onsite reservoirs, because in comparison to onsite reservoirs, watershed areas are smaller and fewer pollutants are collected; moreover, cleaner water is introduced. However, in Taiwan, the water quality of some off-site reservoirs can gradually worsen, and this factor needs to be addressed. In this study, the Liyutan reservoir in central Taiwan was used as an example to demonstrate the process of evaluating pollution in an off-site reservoir. Pollution loads from point sources (PSs) and nonpoint sources (NPSs) were carefully estimated. Domestic sewage and tourist wastewater were considered the major PS loads in this study. The NPS load evaluation was dependent on the results of a verified watershed model, the stormwater management model (SWMM). The observed data in 2015 and 2016 and supplementary total phosphorous (TP) samplings in upstream rivers in 2018 were used to validate the model results. Model calibration and verification were implemented during dry weather and wet weather to ensure the accuracy of the PS and NPS simulations. The results of this study showed that the average total phosphorous (TP) load generated from within the watershed was 9013 kg/y, and that the TP load from outside the watershed was 4545 kg/y. The percentages of TP loads from NPSs and PSs in the watershed were 83% and 17%, respectively. Finally, we used a verified Vollenweider model to convert the TP loads to the TP concentration in the reservoir. The pollution reduction measures and the associated predicted water quality values were assessed using the verified models. [ABSTRACT FROM AUTHOR]
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- 2019
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11. The phosphatase DUSP22 inhibits UBR2-mediated K63-ubiquitination and activation of Lck downstream of TCR signalling.
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Shih, Ying-Chun, Chen, Hsueh-Fen, Wu, Chia-Ying, Ciou, Yi-Ru, Wang, Chia-Wen, Chuang, Huai-Chia, and Tan, Tse-Hua
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REGULATORY T cells ,MITOGEN-activated protein kinase phosphatases ,T cell receptors ,T cells ,GENE expression ,BLOOD cells ,UBIQUITIN ligases - Abstract
DUSP22 is a dual-specificity phosphatase that inhibits T cell activation by inactivating the kinase Lck. Here we show that the E3 ubiquitin ligase UBR2 is a positive upstream regulator of Lck during T-cell activation. DUSP22 dephosphorylates UBR2 at specific Serine residues, leading to ubiquitin-mediated UBR2 degradation. UBR2 is also modified by the SCF E3 ubiquitin ligase complex via Lys48-linked ubiquitination at multiple Lysine residues. Single-cell RNA sequencing analysis and UBR2 loss of function experiments showed that UBR2 is a positive regulator of proinflammatory cytokine expression. Mechanistically, UBR2 induces Lys63-linked ubiquitination of Lck at Lys99 and Lys276 residues, followed by Lck Tyr394 phosphorylation and activation as part of TCR signalling. Inflammatory phenotypes induced by TCR-triggered Lck activation or knocking out DUSP22, are attenuated by genomic deletion of UBR2. UBR2-Lck interaction and Lck Lys63-linked ubiquitination are induced in the peripheral blood T cells of human SLE patients, which demonstrate the relevance of the UBR2-mediated regulation of inflammation to human pathology. In summary, we show here an important regulatory mechanism of T cell activation, which finetunes the balance between T cell response and aggravated inflammation. The T cell receptor signalosome integrates multiple positive and negative regulatory elements to finetune the response and limit harmful inflammation. Here authors show a regulatory cascade of T cell activation, in which DUSP22 negatively regulates UBR2, which is an activator of the kinase Lck via K63 ubiquitination. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Impact of body-mass factors on setup displacement during pelvic irradiation in patients with lower abdominal cancer.
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Wu, Wei-Chieh, Chang, Yi-Ru, Lai, Yo-Liang, Shiau, An-Cheng, Liang, Ji-An, Chien, Chun-Ru, Kuo, Yu-Cheng, and Chen, Shang-Wen
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HIP joint ,ABDOMINAL tumors ,ANTHROPOMETRY ,COMPUTED tomography ,COMPUTERS in medicine ,PATIENT positioning ,PELVIC tumors ,RADIOTHERAPY ,SPACE perception ,LOGISTIC regression analysis ,BODY mass index ,RECEIVER operating characteristic curves ,ANATOMY - Abstract
Background: The aim of the study was investigate the impact of body-mass factors (BMF) on setup displacement during pelvic radiotherapy in patients with lower abdominal cancers. Patients and methods: The clinical data of a training cohort composed of 60 patients with gynecological, rectal, or prostate cancer were analyzed. The daily alignment data from image-guided radiotherapy (IGRT) were retrieved. Setup errors for were assessed by systematic error (SE) and random error (RE) through the superior-inferior (SI), anterior-posterior (AP), and medial-lateral (ML) directions. Several BMFs and patient-related parameters were analyzed with binary logistic regression and receiver-operating characteristic curves. A scoring system was proposed to identify those with greater setup displacement during daily treatment. The results were validated by another cohort. Results: A large hip lateral diameter correlated with a greater SI-SE and AP-SE, whereas a large umbilical AP diameter correlated with a greater ML-SE and ML-RE. A higher SI-RE was associated with a large hip circumference. The positive predictors for setup uncertainty were chosen to dichotomize patients into groups at high risk and low risk for setup displacement. Based on the scoring system, the adequate treatment margins for the SI direction in the high-and low-risk groups were 5.4 mm and 3.8 mm, whereas those for the ML direction were 8.2 mm and 4.2 mm, respectively. The validated cohort showed a similar trend. Conclusions: Large BMFs including hip lateral diameter, hip circumference, and umbilical AP diameter are associated with greater setup uncertainty. Based on the scores, IGRT or required treatment margins can be adapted for patients with high risk features. [ABSTRACT FROM AUTHOR]
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- 2019
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13. Apelin attenuates TGF-β1-induced epithelial to mesenchymal transition via activation of PKC-ε in human renal tubular epithelial cells.
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Wang, Li-Yan, Diao, Zong-Li, Zheng, Jun-Fang, Wu, Yi-Ru, Zhang, Qi-Dong, and Liu, Wen-Hu
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APELIN , *TRANSFORMING growth factors , *EPITHELIAL cells , *PROTEIN kinase C , *CELL differentiation - Abstract
Epithelial to mesenchymal transition (EMT), a process whereby fully differentiated epithelial cells transition to a mesenchymal phenotype, has been implicated in the pathogenesis of renal fibrosis. Apelin, a bioactive peptide, has recently been recognized to protect against renal profibrotic activity, but the underlying mechanism has not yet been elucidated. In this study, we investigated the regulation of EMT in the presence of apelin-13 in vitro. Expression of the mesenchymal marker alpha-smooth muscle actin (α-SMA) and the epithelial marker E-cadherin was examined by immunofluorescence and western blotting in transforming growth factor beta 1 (TGF-β1)-stimulated human proximal tubular epithelial cells. Expression of extracellular matrix, fibronectin and collagen-I was examined by quantitative real-time PCR and ELISA. F13A, an antagonist of the apelin receptor APJ, and small interfering RNA targeting protein kinase C epsilon (PKC-ε) were used to explore the relevant signaling pathways. Apelin attenuated TGF-β1-induced EMT, and inhibited the EMT-associated increase in α-SMA, loss of E-cadherin, and secretion of extracellular matrix. Moreover, apelin activated PKC-ε in tubular epithelial cells, which in turn decreased phospho-Smad2/3 levels and increased Smad-7 levels. APJ inhibition or PKC-ε deletion diminished apelin-induced modulation of Smad signaling and suppression of tubular EMT. Our findings identify a novel PKC-ε-dependent mechanism in which apelin suppresses TGF-β1-mediated activation of Smad signaling pathways and thereby inhibits tubular EMT. These results suggest that apelin may be a new agent that can suppress renal fibrosis and retard chronic kidney disease progression. [ABSTRACT FROM AUTHOR]
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- 2017
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14. Lanzhou University Reports Findings in African Swine Fever Virus (Deletion of DP148R, DP71L, and DP96R Attenuates African Swine Fever Virus, and the Mutant Strain Confers Complete Protection against Homologous Challenges in Pigs).
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AFRICAN swine fever virus ,DNA virus diseases ,ACTINOBACILLUS pleuropneumoniae ,AFRICAN swine fever ,SWINE ,ANIMAL disease control - Abstract
Keywords: Lanzhou; People's Republic of China; Asia; African Swine Fever; African Swine Fever Virus; Arbovirus Infections; Asfarviridae; DNA Virus Infections; DNA Viruses; Genetics; Swine Diseases and Conditions; Tick-Borne Diseases and Conditions; Viral RNA; Virology; Viruses EN Lanzhou People's Republic of China Asia African Swine Fever African Swine Fever Virus Arbovirus Infections Asfarviridae DNA Virus Infections DNA Viruses Genetics Swine Diseases and Conditions Tick-Borne Diseases and Conditions Viral RNA Virology Viruses 33 33 1 04/17/23 20230417 NES 230417 2023 APR 17 (NewsRx) -- By a News Reporter-Staff News Editor at Veterinary Week -- New research on DNA Viruses - African Swine Fever Virus is the subject of a report. Lanzhou, People's Republic of China, Asia, African Swine Fever, African Swine Fever Virus, Arbovirus Infections, Asfarviridae, DNA Virus Infections, DNA Viruses, Genetics, Swine Diseases and Conditions, Tick-Borne Diseases and Conditions, Viral RNA, Virology, Viruses These findings indicate that the deletion mutant virus ASFV-GS-D18R/NL/UK is a novel potential live attenuated vaccine candidate and one of the few experimental vaccine strains reported to induce full protection against the highly virulent ASFV-GS virus strain. [Extracted from the article]
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- 2023
15. Targeting P53 as a Future Strategy to Overcome Gemcitabine Resistance in Biliary Tract Cancers.
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Wu, Chiao-En, Pan, Yi-Ru, Yeh, Chun-Nan, and Lunec, John
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BILIARY tract cancer ,P53 protein ,CELL cycle proteins ,ATAXIA telangiectasia ,ANTINEOPLASTIC agents ,COMBINATION drug therapy - Abstract
Gemcitabine-based chemotherapy is the current standard treatment for biliary tract cancers (BTCs) and resistance to gemcitabine remains the clinical challenge. TP53 mutation has been shown to be associated with poor clinicopathologic characteristics and survival in patients with BTCs, indicating that p53 plays an important role in the treatment of these cancers. Herein, we comprehensively reviewed previous BTC preclinical research and early clinical trials in terms of p53, as well as novel p53-targeted treatment, alone or in combination with either chemotherapy or other targeted therapies in BTCs. Preclinical studies have demonstrated that p53 mutations in BTCs are associated with enhanced gemcitabine resistance, therefore targeting p53 may be a novel therapeutic strategy for treatment of BTCs. Directly targeting mutant p53 by p53 activators, or indirectly by targeting cell cycle checkpoint proteins (Chk1, ataxia telangiectasia related (ATR), and Wee1) leading to synthetic lethality, may be potential future strategies for gemcitabine-resistant p53 mutated BTCs. In contrast, for wild-type p53 BTCs, activation of p53 by inhibition of its negative regulators (MDM2 and wild-type p53-induced phosphatase 1 (WIP1)) may be alternative options. Combination therapies consisting of standard cytotoxic drugs and novel small molecules targeting p53 and related signaling pathways may be the future key standard approach to beat cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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16. Intravenous Arginine Administration Benefits CD4+ T-Cell Homeostasis and Attenuates Liver Inflammation in Mice with Polymicrobial Sepsis.
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Yeh, Chiu-Li, Tanuseputero, Sharon Angela, Wu, Jin-Ming, Tseng, Yi-Ru, Yang, Po-Jen, Lee, Po-Chu, Yeh, Sung-Ling, and Lin, Ming-Tsan
- Abstract
This study investigated the effects of a single dose of arginine (Arg) administration at the beginning of sepsis on CD4
+ T-cell regulation and liver inflammation in C57BL/6J mice. Mice were divided into normal control (NC), sham (SH), sepsis saline (SS), and sepsis Arg (SA) groups. An inducible nitric oxide (NO) synthase (iNOS) inhibitor was administered to additional sepsis groups to evaluate the role of NO during sepsis. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg (300 mg/kg body weight) via tail vein 1 h after CLP. Mice were euthanized at 12 and 24 h post-CLP. Blood, para-aortic lymph nodes, and liver tissues were collected for further measurement. The findings showed that sepsis resulted in decreases in blood and para-aortic lymph node CD4+ T-cell percentages, whereas percentages of interleukin (IL)-4- and IL-17-expressing CD4+ T cells were upregulated. Compared to the SS group, Arg administration resulted in maintained circulating and para-aortic lymph node CD4+ T cells, an increased Th1/Th2 ratio, and a reduced Th17/Treg ratio post-CLP. In addition, levels of plasma liver injury markers and expression of inflammatory genes in liver decreased. These results suggest that a single dose of Arg administered after CLP increased Arg availability, sustained CD4+ T-cell populations, elicited more-balanced Th1/Th2/Th17/Treg polarization in the circulation and the para-aortic lymph nodes, and attenuated liver inflammation in sepsis. The favorable effects of Arg were abrogated when an iNOS inhibitor was administered, which indicated that NO may be participated in regulating the homeostasis of Th/Treg cells and subsequent liver inflammation during sepsis. [ABSTRACT FROM AUTHOR]- Published
- 2020
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17. A Dictionary of High Frequency Function Words in Literary Chinese
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Mei Ah Tan and Mei Ah Tan
- Subjects
- Chinese language--Function words--Dictionaries, Chinese language--Word frequency--Dictionaries, Chinese language--Study and teaching--English speakers
- Abstract
A Dictionary of High Frequency Function Words in Literary Chinese is the first comprehensive work on the subject that constitutes a new approach to teaching and learning by providing both a reference tool and a reader.This dictionary can serve both as a reference book and as an anthology for teaching and learning literary Chinese (the premodern written language) and both ancient and contemporary Chinese culture. It differs from the traditional design of dictionaries in that it includes detailed explanations, with examples, for different uses of the graphs most often used to represent function words in literary Chinese. To facilitate teaching and learning through association, the early meaning, extended meanings, and borrowed meanings for each graph are provided, along with explanations supported by the various stages of the historical development of the graph and other relevant research. Each word is grouped into the primary word class to which it belongs, based on its lexical or grammatical meanings, its collocations, and its function in a sentence. Modern idioms derived from words and phrases in the sample passages are provided in order to promote cultural knowledge and show the important role literary Chinese continues to play in everyday life. All Chinese words are provided with Putonghua romanization to facilitate learning and recall through sound; special pronunciations are explained. This book can be used as a textbook, as extended reading, or as a reference work for undergraduate or graduate classes on literary/classical Chinese, Chinese manuscript studies, Chinese language and culture, Putonghua, translation studies, or even linguistics. It can also be used as an anthology from which the teacher can select passages for students to study.
- Published
- 2023
18. Researchers from Hebei North University Report Findings in Biosensors and Bioelectronics (Multifunctional Fluorescence Probe for Simultaneous Detection of Viscosity, Polarity, and Onoo- and Its Bioimaging In Vitro and Vivo)
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Medical equipment -- Research -- Reports -- Investigations ,Biosensors -- Investigations -- Reports -- Research ,Fluorescence -- Reports -- Research -- Investigations ,Physiological apparatus -- Research -- Reports -- Investigations ,Company legal issue ,Biotechnology industry ,Pharmaceuticals and cosmetics industries - Abstract
2024 JUN 19 (NewsRx) -- By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biotechnology - Biosensors and Bioelectronics have been presented. According to news originating [...]
- Published
- 2024
19. Organic Solutes, Oxidative Stress, and Antioxidant Enzymes Under Abiotic Stressors
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Arafat Abdel Hamed Abdel Latef and Arafat Abdel Hamed Abdel Latef
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- Crops--Effect of stress on, Plants--Effect of stress on, Oxidative stress, Antioxidants
- Abstract
This book presents evidence-based approaches and techniques used to diagnose and manage organic solutes, oxidative stress, and antioxidant enzymes in crop plants under abiotic stressors. It discusses strategies in abiotic stress tolerance including osmoregulation, osmoprotectants, and the regulation of compatible solutes and antioxidant enzymes in plants. With contributions from 49 scholars worldwide, this authoritative guide is educational for scientists working with plants and abiotic stressors. Provides comprehensive coverage of all aspects of abiotic stress, from abiotic stresses'effects on plant growth, development, and defense mechanisms, to functionality of enzymatic and non-enzymatic antioxidant enzymes in crop plants. Outlines the dangers of reactive oxygen species. Discusses using antioxidant enzymes and antioxidant molecules in plant protection mechanisms. Edited by Arafat Abdel Hamed Abdel Latef, Professor of Plant Physiology at South Valley University, Egypt, this book is written for graduate students and scholars researching abiotic plant stressors. “The book represents an excellent strategy to understand the mechanisms and techniques of antioxidant enzymes in the plant cell under stress conditions.” – Professor Mostafa El-sheekh“Provides a thorough and detailed picture of the updated knowledge on the techniques used to manage organic solutes, oxidative stress and stress-related enzymes under abiotic stressors.” – Bhoopander Giri, Ph.D.“Will serve as an imperative source of scientific literature in the plant stress biology field.” – Narendra Singh Yadav, Ph.D.“The book has eighteen chapters written by scholars of international expertise in plant stress management.” – Dr. Sikander PAL, Senior Assistant Professor
- Published
- 2021
20. Annual Report on The Development of China's Special Economic Zones (2017) : Blue Book of China's Special Economic Zones
- Author
-
Yitao Tao, Yiming Yuan, Yitao Tao, and Yiming Yuan
- Subjects
- Asia—Economic conditions, Microeconomics, Development economics
- Abstract
This Report objectively reflects the whole year's progress of politics, economy, society, culture, system, environment, innovation and reform as well as the problems, challenges and countermeasures in traditional special economic zones and new special economic zones. It makes analysis of China's Special Economic Zones, including overall review on the whole year's development state of the reform experimental zone and part of new special economic zones, which focuses on analyzing the transformation of special economic zones, use of resources, the sustainable development, economic and social development, social security and technical innovation from the aspects of present situation of development, the comparative analysis, and policy suggestions and puts forward development suggestions for each specific issue.
- Published
- 2019
21. Engineering Tribology and Materials II
- Author
-
Yunn Lin Hwang and Yunn Lin Hwang
- Subjects
- Tribology--Congresses
- Abstract
3rd International Conference on Engineering Tribology and Applied Technology (ICETAT 2017)Selected, peer reviewed papers from the International Conference on Engineering Tribology and Applied Technology 2017 (ICETAT2017), October 6-7, 2017, Tainan, Taiwan.
- Published
- 2018
22. Recent Research from Guangzhou Medical University Highlight Findings in Neuromuscular Manifestations (The Possible Role of Hypothalamus-pituitary-adrenal Dysfunction In Epileptic Spasms)
- Subjects
Medical research -- Analysis ,Medicine, Experimental -- Analysis ,ACTH -- Research ,Physical fitness -- Research -- Analysis ,Health - Abstract
2020 DEC 26 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators discuss new findings in Musculoskeletal Diseases and Conditions - Neuromuscular Manifestations. [...]
- Published
- 2020
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