189 results on '"Purahong, Witoon"'
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2. Active soil microbial composition and proliferation are directly affected by the presence of biocides from building materials
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Reiß, Fabienne, Kiefer, Nadine, Purahong, Witoon, Borken, Werner, Kalkhof, Stefan, and Noll, Matthias
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- 2024
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3. Future climate conditions accelerate wheat straw decomposition alongside altered microbial community composition, assembly patterns, and interaction networks
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Wahdan, Sara Fareed Mohamed, Ji, Li, Schädler, Martin, Wu, Yu-Ting, Sansupa, Chakriya, Tanunchai, Benjawan, Buscot, François, and Purahong, Witoon
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- 2023
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4. FungalTraits vs. FUNGuild: Comparison of Ecological Functional Assignments of Leaf- and Needle-Associated Fungi Across 12 Temperate Tree Species
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Tanunchai, Benjawan, Ji, Li, Schroeter, Simon Andreas, Wahdan, Sara Fareed Mohamed, Hossen, Shakhawat, Delelegn, Yoseph, Buscot, François, Lehnert, Ann-Sophie, Alves, Eliane Gomes, Hilke, Ines, Gleixner, Gerd, Schulze, Ernst-Detlef, Noll, Matthias, and Purahong, Witoon
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- 2023
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5. Fate of a biodegradable plastic in forest soil: Dominant tree species and forest types drive changes in microbial community assembly, influence the composition of plastisphere, and affect poly(butylene succinate-co-adipate) degradation
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Tanunchai, Benjawan, Ji, Li, Schröder, Olaf, Gawol, Susanne Julia, Geissler, Andreas, Wahdan, Sara Fareed Mohamed, Buscot, François, Kalkhof, Stefan, Schulze, Ernst-Detlef, Noll, Matthias, and Purahong, Witoon
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- 2023
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6. Nitrogen addition increases mass loss of gymnosperm but not of angiosperm deadwood without changing microbial communities
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Roy, Friederike, Ibayev, Orkhan, Arnstadt, Tobias, Bässler, Claus, Borken, Werner, Groß, Christina, Hoppe, Björn, Hossen, Shakhawat, Kahl, Tiemo, Moll, Julia, Noll, Matthias, Purahong, Witoon, Schreiber, Jasper, Weisser, Wolfgang W., Hofrichter, Martin, and Kellner, Harald
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- 2023
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7. Interactions Between High Load of a Bio-based and Biodegradable Plastic and Nitrogen Fertilizer Affect Plant Biomass and Health: A Case Study with Fusarium solani and Mung Bean (Vigna radiata L.)
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Scheid, Sarah-Maria, Juncheed, Kantida, Tanunchai, Benjawan, Wahdan, Sara Fareed Mohamed, Buscot, François, Noll, Matthias, and Purahong, Witoon
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- 2022
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8. Fungal community succession on decomposing leaf litter across five phylogenetically related tree species in a subtropical forest
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Tennakoon, Danushka S., Kuo, Chang-Hsin, Purahong, Witoon, Gentekaki, Eleni, Pumas, Chayakorn, Promputtha, Itthayakorn, and Hyde, Kevin D.
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- 2022
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9. Life in the Wheat Litter: Effects of Future Climate on Microbiome and Function During the Early Phase of Decomposition
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Wahdan, Sara Fareed Mohamed, Hossen, Shakhawat, Tanunchai, Benjawan, Sansupa, Chakriya, Schädler, Martin, Noll, Matthias, Dawoud, Turki M., Wu, Yu-Ting, Buscot, François, and Purahong, Witoon
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- 2022
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10. Taxonomical and functional composition of strawberry microbiome is genotype-dependent
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Sangiorgio, Daniela, Cellini, Antonio, Donati, Irene, Ferrari, Erika, Tanunchai, Benjawan, Fareed Mohamed Wahdan, Sara, Sadubsarn, Dolaya, Farneti, Brian, Checcucci, Alice, Buscot, François, Spinelli, Francesco, and Purahong, Witoon
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- 2022
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11. City life of mycorrhizal and wood-inhabiting macrofungi: Importance of urban areas for maintaining fungal biodiversity
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Purahong, Witoon, Günther, Angela, Gminder, Andreas, Tanunchai, Benjawan, Gossner, Martin M., Buscot, François, and Schulze, Ernst-Detlef
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- 2022
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12. Home-Field Advantage in Wood Decomposition Is Mainly Mediated by Fungal Community Shifts at “Home” Versus “Away”
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Purahong, Witoon, Kahl, Tiemo, Krüger, Dirk, Buscot, François, and Hoppe, Björn
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- 2019
13. Community Assembly Processes of Deadwood Mycobiome in a Tropical Forest Revealed by Long-Read Third-Generation Sequencing.
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Purahong, Witoon, Ji, Li, and Wu, Yu-Ting
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TROPICAL forests , *DETERMINISTIC processes , *STOCHASTIC processes , *NUTRIENT cycles , *FUNGAL communities , *WOOD - Abstract
Despite the importance of wood-inhabiting fungi on nutrient cycling and ecosystem functions, their ecology, especially related to their community assembly, is still highly unexplored. In this study, we analyzed the wood-inhabiting fungal richness, community composition, and phylogenetics using PacBio sequencing. Opposite to what has been expected that deterministic processes especially environmental filtering through wood-physicochemical properties controls the community assembly of wood-inhabiting fungal communities, here we showed that both deterministic and stochastic processes can highly contribute to the community assembly processes of wood-inhabiting fungi in this tropical forest. We demonstrated that the dynamics of stochastic and deterministic processes varied with wood decomposition stages. The initial stage was mainly governed by a deterministic process (homogenous selection), whereas the early and later decomposition stages were governed by the stochastic processes (ecological drift). Deterministic processes were highly contributed by wood physicochemical properties (especially macronutrients and hemicellulose) rather than soil physicochemical factors. We elucidated that fine-scale fungal-fungal interactions, especially the network topology, modularity, and keystone taxa of wood-inhabiting fungal communities, strongly differed in an initial and decomposing deadwood. This current study contributes to a better understanding of the ecological processes of wood-inhabiting fungi in tropical regions where the knowledge of wood-inhabiting fungi is highly limited. [ABSTRACT FROM AUTHOR]
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- 2024
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14. DNA of Music: Identifying Relationships among Different Versions of the Composition Sadhukarn from Thailand, Laos, and Cambodia Using Multivariate Statistics.
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Eambangyung, Sumetus, Schwörer-Kohl, Gretel, and Purahong, Witoon
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COUNTRY of origin (Immigrants) ,MULTIDIMENSIONAL scaling ,MUSICAL composition ,SACRED music ,STATISTICS - Abstract
Sadhukarn, a sacred music composition performed ritually to salute and invite divine powers to open a ceremony or feast, is played in Thailand, Cambodia, and Laos. Different countries have unique versions, arranged based on musicians' skills and en vogue styles. This study presents the results of multivariate statistical analyses of 26 different versions of Sadhukarn main melodies using non-metric multidimensional scaling (NMDS) and cluster analysis. The objective was to identify the optimal number of parameters for identifying the origin and relationships among Sadhukarn versions, including rhyme structures, pillar tone, rhythmic and melodic patterns, intervals, pitches, and combinations of these parameters. The data were analyzed using both full and normalized datasets (32 phrases) to avoid biases due to differences in phrases among versions. Overall, the combination of six parameters is the best approach for data analysis in both full and normalized datasets. The analysis of the 'full version' shows the separation of Sadhukarn versions from different countries of origin, while the analysis of the 'normalized version' reveals the rhyme structure, rhythmic structure, and pitch as crucial parameters for identifying Sadhukarn versions. We conclude that multivariate statistics are powerful tools for identifying relationships among different versions of Sadhukarn compositions from Thailand, Laos, and Cambodia and within the same countries of origin. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Analysis of microbial populations in plastic–soil systems after exposure to high poly(butylene succinate-co-adipate) load using high-resolution molecular technique
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Tanunchai, Benjawan, Juncheed, Kantida, Wahdan, Sara Fareed Mohamed, Guliyev, Vusal, Udovenko, Maria, Lehnert, Ann-Sophie, Alves, Eliane Gomes, Glaser, Bruno, Noll, Matthias, Buscot, François, Blagodatskaya, Evgenia, and Purahong, Witoon
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- 2021
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16. Taxonomic and phylogenetic contributions to fungi associated with the invasive weed Chromolaena odorata (Siam weed)
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Mapook, Ausana, Hyde, Kevin D., McKenzie, Eric H. C., Jones, E. B. Gareth, Bhat, D. Jayarama, Jeewon, Rajesh, Stadler, Marc, Samarakoon, Milan C., Malaithong, Maitree, Tanunchai, Benjawan, Buscot, François, Wubet, Tesfaye, and Purahong, Witoon
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- 2020
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17. Tree mycorrhizal type regulates leaf and needle microbial communities, affects microbial assembly and co-occurrence network patterns, and influences litter decomposition rates in temperate forest B.
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Tanunchai, Benjawan, Li Ji, Schroeter, Simon Andreas, Mohamed Wahdan, Sara Fareed, Thongsuk, Katikarn, Hilke, Ines, Gleixner, Gerd, Buscot, François, Schulze, Ernst-Detlef, Noll, Matthias, and Purahong, Witoon
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MICROBIAL communities ,TEMPERATE forests ,MICROBIAL diversity ,FOREST litter ,FUNGAL communities ,VESICULAR-arbuscular mycorrhizas ,ECTOMYCORRHIZAL fungi - Abstract
Background: Tree mycorrhizal types (arbuscular mycorrhizal fungi and ectomycorrhizal fungi) alter nutrient use traits and leaf physicochemical properties and, thus, affect leaf litter decomposition. However, little is known about how different tree mycorrhizal species affect the microbial diversity, community composition, function, and community assembly processes that govern leaf litter-dwelling microbes during leaf litter decomposition. Methods: In this study, we investigated the microbial diversity, community dynamics, and community assembly processes of nine temperate tree species using high-resolution molecular technique (Illumina sequencing), including broadleaved arbuscular mycorrhizal, broadleaved ectomycorrhizal, and coniferous ectomycorrhizal tree types, during leaf litter decomposition. Results and discussion: The leaves and needles of different tree mycorrhizal types significantly affected the microbial richness and community composition during leaf litter decomposition. Leaf litter mass loss was related to higher sequence reads of a few bacterial functional groups, particularly N-fixing bacteria. Furthermore, a link between bacterial and fungal community composition and hydrolytic and/or oxidative enzyme activity was found. The microbial communities in the leaf litter of different tree mycorrhizal types were governed by different proportions of determinism and stochasticity, which changed throughout litter decomposition. Specifically, determinism (mainly variable selection) controlling bacterial community composition increased over time. In contrast, stochasticity (mainly ecological drift) increasingly governed fungal community composition. Finally, the co-occurrence network analysis showed greater competition between bacteria and fungi in the early stages of litter decomposition and revealed a contrasting pattern between mycorrhizal types. Conclusion: Overall, we conclude that tree mycorrhizal types influence leaf litter quality, which affects microbial richness and community composition, and thus, leaf litter decomposition. [ABSTRACT FROM AUTHOR]
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- 2023
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18. Can we use environmental DNA as holotypes?
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Hongsanan, Sinang, Jeewon, Rajesh, Purahong, Witoon, Xie, Ning, Liu, Jian-Kui, Jayawardena, Ruvishika S., Ekanayaka, Anusha H., Dissanayake, Asha, Raspé, Olivier, Hyde, Kevin D., Stadler, Marc, and Peršoh, Derek
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- 2018
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19. Life on the wall: the diversity and activity of microbes on 13th - century AD. Lan Na mural painting.
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Sansupa, Chakriya, Suphaphimol, Nattaphon, Nonthijun, Paradha, Ronsuek, Teewararat, Yimklan, Saranphong, Semakul, Natthawat, Khrueraya, Thapakorn, Suwannarach, Nakarin, Purahong, Witoon, and Disayathanoowat, Terd
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MURAL art ,CALCIUM compounds ,FUNGAL communities ,MICROORGANISMS ,BIODEGRADATION ,ARCHAEBACTERIA - Abstract
Diverse microorganisms from the three domains of life (Archaea, Bacteria, and Eukaryota) cause deterioration in mural paintings worldwide; however, few studies have simultaneously targeted these three domains. This study aims to survey the microbiome and its potential for biodeterioration on unpreserved Lan Na mural paintings in Sean Khan temple, Chiang Mai, Thailand. The overview of the archaeal, bacterial, and fungal communities was reported by Illumina sequencing, whereas the potential for deterioration was revealed by culturable techniques and a literature search. The abundant microbes reported in this study were also found in other ancient mural paintings worldwide. Halococcus, a salt-tolerant archaeon, as well as the eubacterial genus Crossiella dominated the prokaryotic community. On the other hand, the main fungal group was the genus Candida (Ascomycota). However, a low number of fungi and bacteria were isolated. Most of the isolates showed the ability to survive in the drought conditions of mural paintings but could not perform discoloration activities. The deterioration activity mainly affected calcium compounds, which are the main components of painting substrates. Aspergillus and several bacterial isolates could dissolve calcium compounds, but only Trichaptum species could induce crystal formation. These results suggest that deterioration of painting substrate should be taken into consideration in addition to deterioration of color in mural paintings. For the Lan Na painting in Sean Khan temple, the plaster is the prime target for biodeterioration, and thus we suggest that the preservation effort should focus on this component of the mural painting. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Considerations and consequences of allowing DNA sequence data as types of fungal taxa
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Zamora, Juan Carlos, Svensson, Måns, Kirschner, Roland, Olariaga, Ibai, Ryman, Svengunnar, Parra, Luis Alberto, Geml, József, Rosling, Anna, Adamčík, Slavomír, Ahti, Teuvo, Aime, M. Catherine, Ainsworth, A. Martyn, Albert, László, Albertó, Edgardo, García, Alberto Altés, Ageev, Dmitry, Agerer, Reinhard, Aguirre-Hudson, Begoña, Ammirati, Joe, Andersson, Harry, Angelini, Claudio, Antonín, Vladimír, Aoki, Takayuki, Aptroot, André, Argaud, Didier, Sosa, Blanca Imelda Arguello, Aronsen, Arne, Arup, Ulf, Asgari, Bita, Assyov, Boris, Atienza, Violeta, Bandini, Ditte, Baptista-Ferreira, João Luís, Baral, Hans-Otto, Baroni, Tim, Barreto, Robert Weingart, Beker, Henry, Bell, Ann, Bellanger, Jean-Michel, Bellù, Francesco, Bemmann, Martin, Bendiksby, Mika, Bendiksen, Egil, Bendiksen, Katriina, Benedek, Lajos, Bérešová-Guttová, Anna, Berger, Franz, Berndt, Reinhard, Bernicchia, Annarosa, Biketova, Alona Yu., Bizio, Enrico, Bjork, Curtis, Boekhout, Teun, Boertmann, David, Böhning, Tanja, Boittin, Florent, Boluda, Carlos G., Boomsluiter, Menno W., Borovička, Jan, Brandrud, Tor Erik, Braun, Uwe, Brodo, Irwin, Bulyonkova, Tatiana, Burdsall, Jr., Harold H., Buyck, Bart, Burgaz, Ana Rosa, Calatayud, Vicent, Callac, Philippe, Campo, Emanuele, Candusso, Massimo, Capoen, Brigitte, Carbó, Joaquim, Carbone, Matteo, Castañeda-Ruiz, Rafael F., Castellano, Michael A., Chen, Jie, Clerc, Philippe, Consiglio, Giovanni, Corriol, Gilles, Courtecuisse, Régis, Crespo, Ana, Cripps, Cathy, Crous, Pedro W., da Silva, Gladstone Alves, da Silva, Meiriele, Dam, Marjo, Dam, Nico, Dämmrich, Frank, Das, Kanad, Davies, Linda, De Crop, Eske, De Kesel, Andre, De Lange, Ruben, De Madrignac Bonzi, Bárbara, dela Cruz, Thomas Edison E., Delgat, Lynn, Demoulin, Vincent, Desjardin, Dennis E., Diederich, Paul, Dima, Bálint, Dios, Maria Martha, Divakar, Pradeep Kumar, Douanla-Meli, Clovis, Douglas, Brian, Drechsler-Santos, Elisandro Ricardo, Dyer, Paul S., Eberhardt, Ursula, Ertz, Damien, Esteve-Raventós, Fernando, Salazar, Javier Angel Etayo, Evenson, Vera, Eyssartier, Guillaume, Farkas, Edit, Favre, Alain, Fedosova, Anna G., Filippa, Mario, Finy, Péter, Flakus, Adam, Fos, Simón, Fournier, Jacques, Fraiture, André, Franchi, Paolo, Molano, Ana Esperanza Franco, Friebes, Gernot, Frisch, Andreas, Fryday, Alan, Furci, Giuliana, Márquez, Ricardo Galán, Garbelotto, Matteo, García-Martín, Joaquina María, Otálora, Mónica A. García, Sánchez, Dania García, Gardiennet, Alain, Garnica, Sigisfredo, Benavent, Isaac Garrido, Gates, Genevieve, da Gerlach, Alice Cruz Lima, Ghobad-Nejhad, Masoomeh, Gibertoni, Tatiana B., Grebenc, Tine, Greilhuber, Irmgard, Grishkan, Bella, Groenewald, Johannes Z., Grube, Martin, Gruhn, Gérald, Gueidan, Cécile, Gulden, Gro, Gusmão, Luis F. P., Hafellner, Josef, Hairaud, Michel, Halama, Marek, Hallenberg, Nils, Halling, Roy E., Hansen, Karen, Harder, Christoffer Bugge, Heilmann-Clausen, Jacob, Helleman, Stip, Henriot, Alain, Hernandez-Restrepo, Margarita, Herve, Raphaël, Hobart, Caroline, Hoffmeister, Mascha, Høiland, Klaus, Holec, Jan, Holien, Håkon, Hughes, Karen, Hubka, Vit, Huhtinen, Seppo, Ivančević, Boris, Jagers, Marian, Jaklitsch, Walter, Jansen, AnnaElise, Jayawardena, Ruvishika S., Jeppesen, Thomas Stjernegaard, Jeppson, Mikael, Johnston, Peter, Jørgensen, Per Magnus, Kärnefelt, Ingvar, Kalinina, Liudmila B., Kantvilas, Gintaras, Karadelev, Mitko, Kasuya, Taiga, Kautmanová, Ivona, Kerrigan, Richard W., Kirchmair, Martin, Kiyashko, Anna, Knapp, Dániel G., Knudsen, Henning, Knudsen, Kerry, Knutsson, Tommy, Kolařík, Miroslav, Kõljalg, Urmas, Košuthová, Alica, Koszka, Attila, Kotiranta, Heikki, Kotkova, Vera, Koukol, Ondřej, Kout, Jiří, Kovács, Gábor M., Kříž, Martin, Kruys, Åsa, Kučera, Viktor, Kudzma, Linas, Kuhar, Francisco, Kukwa, Martin, Kumar, T. K. Arun, Kunca, Vladimír, Kušan, Ivana, Kuyper, Thomas W., Lado, Carlos, Læssøe, Thomas, Lainé, Patrice, Langer, Ewald, Larsson, Ellen, Larsson, Karl-Henrik, Laursen, Gary, Lechat, Christian, Lee, Serena, Lendemer, James C., Levin, Laura, Lindemann, Uwe, Lindström, Håkan, Liu, Xingzhong, Hernandez, Regulo Carlos Llarena, Llop, Esteve, Locsmándi, Csaba, Lodge, Deborah Jean, Loizides, Michael, Lőkös, László, Luangsa-ard, Jennifer, Lüderitz, Matthias, Lumbsch, Thorsten, Lutz, Matthias, Mahoney, Dan, Malysheva, Ekaterina, Malysheva, Vera, Manimohan, Patinjareveettil, Marin-Felix, Yasmina, Marques, Guilhermina, Martínez-Gil, Rubén, Marson, Guy, Mata, Gerardo, Matheny, P. Brandon, Mathiassen, Geir Harald, Matočec, Neven, Mayrhofer, Helmut, Mehrabi, Mehdi, Melo, Ireneia, Mešić, Armin, Methven, Andrew S., Miettinen, Otto, Romero, Ana M. Millanes, Miller, Andrew N., Mitchell, James K., Moberg, Roland, Moreau, Pierre-Arthur, Moreno, Gabriel, Morozova, Olga, Morte, Asunción, Muggia, Lucia, González, Guillermo Muñoz, Myllys, Leena, Nagy, István, Nagy, László G., Neves, Maria Alice, Niemelä, Tuomo, Nimis, Pier Luigi, Niveiro, Nicolas, Noordeloos, Machiel E., Nordin, Anders, Noumeur, Sara Raouia, Novozhilov, Yuri, Nuytinck, Jorinde, Ohenoja, Esteri, Fiuza, Patricia Oliveira, Orange, Alan, Ordynets, Alexander, Ortiz-Santana, Beatriz, Pacheco, Leticia, Pál-Fám, Ferenc, Palacio, Melissa, Palice, Zdeněk, Papp, Viktor, Pärtel, Kadri, Pawlowska, Julia, Paz, Aurelia, Peintner, Ursula, Pennycook, Shaun, Pereira, Olinto Liparini, Daniëls, Pablo Pérez, Capella, Miquel À. Pérez-De-Gregorio, del Amo, Carlos Manuel Pérez, Gorjón, Sergio Pérez, Pérez-Ortega, Sergio, Pérez-Vargas, Israel, Perry, Brian A., Petersen, Jens H., Petersen, Ronald H., Pfister, Donald H., Phukhamsakda, Chayanard, Piątek, Marcin, Piepenbring, Meike, Pino-Bodas, Raquel, Esquivel, Juan Pablo Pinzón, Pirot, Paul, Popov, Eugene S., Popoff, Orlando, Álvaro, María Prieto, Printzen, Christian, Psurtseva, Nadezhda, Purahong, Witoon, Quijada, Luis, Rambold, Gerhard, Ramírez, Natalia A., Raja, Huzefa, Raspé, Olivier, Raymundo, Tania, Réblová, Martina, Rebriev, Yury A., García, Juan de Dios Reyes, Ripoll, Miguel Ángel Ribes, Richard, Franck, Richardson, Mike J., Rico, Víctor J., Robledo, Gerardo Lucio, Barbosa, Flavia Rodrigues, Rodriguez-Caycedo, Cristina, Rodriguez-Flakus, Pamela, Ronikier, Anna, Casas, Luis Rubio, Rusevska, Katerina, Saar, Günter, Saar, Irja, Salcedo, Isabel, Martínez, Sergio M. Salcedo, Montoya, Carlos A. Salvador, Sánchez-Ramírez, Santiago, Sandoval-Sierra, J. Vladimir, Santamaria, Sergi, Monteiro, Josiane Santana, Schroers, Hans Josef, Schulz, Barbara, Schmidt-Stohn, Geert, Schumacher, Trond, Senn-Irlet, Beatrice, Ševčíková, Hana, Shchepin, Oleg, Shirouzu, Takashi, Shiryaev, Anton, Siepe, Klaus, Sir, Esteban B., Sohrabi, Mohammad, Soop, Karl, Spirin, Viacheslav, Spribille, Toby, Stadler, Marc, Stalpers, Joost, Stenroos, Soili, Suija, Ave, Sunhede, Stellan, Svantesson, Sten, Svensson, Sigvard, Svetasheva, Tatyana Yu., Świerkosz, Krzysztof, Tamm, Heidi, Taskin, Hatira, Taudière, Adrien, Tedebrand, Jan-Olof, Lahoz, Raúl Tena, Temina, Marina, Thell, Arne, Thines, Marco, Thor, Göran, Thüs, Holger, Tibell, Leif, Tibell, Sanja, Timdal, Einar, Tkalčec, Zdenko, Tønsberg, Tor, Trichies, Gérard, Triebel, Dagmar, Tsurykau, Andrei, Tulloss, Rodham E., Tuovinen, Veera, Sosa, Miguel Ulloa, Urcelay, Carlos, Valade, François, Garza, Ricardo Valenzuela, van den Boom, Pieter, Van Vooren, Nicolas, Vasco-Palacios, Aida M., Vauras, Jukka, Santos, Juan Manuel Velasco, Vellinga, Else, Verbeken, Annemieke, Vetlesen, Per, Vizzini, Alfredo, Voglmayr, Hermann, Volobuev, Sergey, von Brackel, Wolfgang, Voronina, Elena, Walther, Grit, Watling, Roy, Weber, Evi, Wedin, Mats, Weholt, Øyvind, Westberg, Martin, Yurchenko, Eugene, Zehnálek, Petr, Zhang, Huang, Zhurbenko, Mikhail P., and Ekman, Stefan
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- 2018
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21. Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine (Vitis vinifera)
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Dissanayake, Asha J., Purahong, Witoon, Wubet, Tesfaye, Hyde, Kevin D., Zhang, Wei, Xu, Haiying, Zhang, Guojun, Fu, Chunyuan, Liu, Mei, Xing, Qikai, Li, Xinghong, and Yan, Jiye
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- 2018
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22. Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches
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Jayawardena, Ruvishika S., Purahong, Witoon, Zhang, Wei, Wubet, Tesfaye, Li, XingHong, Liu, Mei, Zhao, Wensheng, Hyde, Kevin D., Liu, JianHua, and Yan, Jiye
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- 2018
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23. Transition of Ethiopian highland forests to agriculture-dominated landscapes shifts the soil microbial community composition
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Delelegn, Yoseph T., Purahong, Witoon, Sandén, Hans, Yitaferu, Birru, Godbold, Douglas L., and Wubet, Tesfaye
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- 2018
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24. Effects of Forest Management Practices in Temperate Beech Forests on Bacterial and Fungal Communities Involved in Leaf Litter Degradation
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Purahong, Witoon, Kapturska, Danuta, Pecyna, Marek J., Jariyavidyanont, Katalee, Kaunzner, Jennifer, Juncheed, Kantida, Uengwetwanit, Tanaporn, Rudloff, Renate, Schulz, Elke, Hofrichter, Martin, Schloter, Michael, Krüger, Dirk, and Buscot, François
- Published
- 2015
25. Reinoculation elucidates mechanisms of bacterial community assembly in soil and reveals undetected microbes
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Francioli, Davide, Schulz, Elke, Purahong, Witoon, Buscot, François, and Reitz, Thomas
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- 2016
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26. Microbial community profiling and culturing reveal functional groups of bacteria associated with Thai commercial stingless worker bees (Tetragonula pagdeni).
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Sinpoo, Chainarong, In-on, Ammarin, Noirungsee, Nuttapol, Attasopa, Korrawat, Chantawannakul, Panuwan, Chaimanee, Veeranan, Phokasem, Patcharin, Ling, Tial Cung, Purahong, Witoon, and Disayathanoowat, Terd
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STINGLESS bees ,MICROBIAL communities ,FUNCTIONAL groups ,BACTERIA - Abstract
Stingless bees play a crucial role in the environment and agriculture as they are effective pollinators. Furthermore, they can produce various products that can be exploited economically, such as propolis and honey. Despite their economic value, the knowledge of microbial community of stingless bees, and their roles on the bees' health, especially in Thailand, are in its infancy. This study aimed to investigate the composition and the functions of bacterial community associated with Tetragonula pagdeni stingless bees using culture-independent and culture-dependent approaches with emphasis on lactic acid bacteria. The culture-independent results showed that the dominant bacterial phyla were Firmicutes, Proteobacteria and Actinobacteria. The most abundant families were Lactobacillaceae and Halomonadaceae. Functional prediction indicated that the prevalent functions of bacterial communities were chemoheterotrophy and fermentation. In addition, the bacterial community might be able to biosynthesize amino acid and antimicrobial compounds. Further isolation and characterization resulted in isolates that belonged to the dominant taxa of the community and possessed potentially beneficial metabolic activity. This suggested that they are parts of the nutrient acquisition and host defense bacterial functional groups in Thai commercial stingless bees. [ABSTRACT FROM AUTHOR]
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- 2023
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27. Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests
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Hoppe, Björn, Purahong, Witoon, Wubet, Tesfaye, Kahl, Tiemo, Bauhus, Jürgen, Arnstadt, Tobias, Hofrichter, Martin, Buscot, François, and Krüger, Dirk
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- 2016
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28. Degradation of Bio-Based and Biodegradable Plastic and Its Contribution to Soil Organic Carbon Stock.
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Guliyev, Vusal, Tanunchai, Benjawan, Udovenko, Maria, Menyailo, Oleg, Glaser, Bruno, Purahong, Witoon, Buscot, François, and Blagodatskaya, Evgenia
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BIODEGRADABLE plastics ,CHERNOZEM soils ,CARBON in soils ,ORGANIC compounds ,STABLE isotopes - Abstract
Expanding the use of environmentally friendly materials to protect the environment is one of the key factors in maintaining a sustainable ecological balance. Poly(butylene succinate-co-adipate) (PBSA) is considered among the most promising bio-based and biodegradable plastics for the future with a high number of applications in soil and agriculture. Therefore, the decomposition process of PBSA and its consequences for the carbon stored in soil require careful monitoring. For the first time, the stable isotope technique was applied in the current study to partitioning plastic- and soil-originated C in the CO
2 released during 80 days of PBSA decomposition in a Haplic Chernozem soil as dependent on nitrogen availability. The decomposition of the plastic was accompanied by the C loss from soil organic matter (SOM) through priming, which in turn was dependent on added N. Nitrogen facilitated PBSA decomposition and reduced the priming effect during the first 6 weeks of the experiment. During the 80 days of plastic decomposition, 30% and 49% of the released CO2 were PBSA-derived, while the amount of SOM-derived CO2 exceeded the corresponding controls by 100.2 and 132.3% in PBSA-amended soil without and with N fertilization, respectively. Finally, only 4.1% and 5.4% of the PBSA added into the soil was mineralized to CO2 , in the treatments without and with N amendment, respectively. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
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29. Comparing fungal richness and community composition in coarse woody debris in Central European beech forests under three types of management
- Author
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Purahong, Witoon, Kahl, Tiemo, Schloter, Michael, Bauhus, Jürgen, Buscot, François, and Krüger, Dirk
- Published
- 2014
- Full Text
- View/download PDF
30. Conversion of rainforest to rubber plantations impacts the rhizosphere soil mycobiome and alters soil biological activity.
- Author
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Monkai, Jutamart, Purahong, Witoon, Nawaz, Ali, Wubet, Tesfaye, Hyde, Kevin D., Goldberg, Stefanie D., Mortimer, Peter E., Xu, Jianchu, and Harrison, Rhett D.
- Subjects
RUBBER plantations ,PLANT competition ,RHIZOSPHERE ,FOREST conversion ,RAIN forests ,SOIL respiration - Abstract
In Southeast Asia and Asia, large swathes of rainforest have been converted to rubber plantations, with major consequences for biodiversity and ecosystem services. However, the impact of this land use conversion on rhizosphere soil mycobiome has not yet been addressed. This study aimed to investigate how rhizosphere soil fungal communities and their associated biological activity (soil respiration, soil methane (CH4), and potential soil enzyme production) are impacted by the conversion of rainforest to rubber plantations. Fungal richness and community composition in rhizosphere soils collected from natural rainforests, immature rubber, and mature rubber plantations were analyzed using paired‐end Illumina sequencing. The conversion of natural rainforest to rubber plantations significantly altered fungal community composition of specific functional groups (saprotrophs, pathogens, and mycorrhiza). We observed significant loss of saprotrophic and ectomycorrhizal fungi in natural rainforests, but enrichment of plant pathogenic fungi in immature rubber plantations. The mechanism underlying the effects of forest conversion on changes in fungal communities is related to reductions in soil pH, total nitrogen (N), and ammonium (NH4) in rubber plantations. Conversion to rubber plantation also resulted in decline of soil respiration rates and less potential for cellulase and chitinase productions. The significant negative correlations between fungal richness and soil respiration in mature rubber plantations indicated high competition among fungi and low nutrient availability in this system. We demonstrate the negative consequences of the conversion of rainforest to rubber plantations on soil biological activity and significant changes in fungal community composition that could threaten long‐term ecosystem functions. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
31. Dark side of a bio-based and biodegradable plastic? Assessment of pathogenic microbes associated with poly(butylene succinate-coadipate) under ambient and future climates using next-generation sequencing.
- Author
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Juncheed, Kantida, Tanunchai, Benjawan, Wahdan, Sara Fareed Mohamed, Thongsuk, Katikarn, Schädler, Martin, Noll, Matthias, and Purahong, Witoon
- Subjects
BIODEGRADABLE plastics ,NUCLEOTIDE sequencing ,PHYTOPATHOGENIC microorganisms ,PLASTIC films ,PLASTIC mulching - Abstract
Bio-based and biodegradable plastic mulching films have been proposed to replace the non-biodegradable plastic mulch films to solve plastic pollution problems in agricultural soils. However, the impact of bio-based and biodegradable plastics on plant and human health remains largely unexplored. Here, we aimed to assess the risk under field conditions of a bio-based and biodegradable poly(butylene succinate-co-adipate; PBSA), a widely used mulching film as carrier of potential pathogenic microorganisms (bacteria and fungi) at ambient and future climate conditions. Overall, we affiliated 64 fungal and 11 bacterial operational taxonomic units (OTUs) as pathogens by using Next-Generation Sequencing approach. Our results revealed that PBSA hosted at least 53 plant pathogens, of which 51 were classified as fungi, while the other two were bacteria. Most fungal plant pathogens were able to withstand the anticipated future climate changes. We detected 13 fungal and eight bacterial OTUs, which were classified as opportunistic human pathogens. Only one bacterial OTU (Enterococcus faecium) was assigned to a human pathogen. While future climate conditions only significantly impacted on the presence and frequency of detection of few pathogens, incubation time was found to significantly impacted on nine pathogens. This result demonstrates the temporal dynamics of pathogens associated with PBSA. The threats to plant and human health were discussed. We emphasize that the risks to human health are relatively low because we mainly found opportunistic pathogens associated with PBSA and the amount are comparable to the plant debris. However, the risks to plant health may be considered as moderate because many plant pathogens were discovered and/or enriched in PBSA. Furthermore, in soil environments, the pathogenic risk of plastic is highly depending on the surrounding soil pathobiome where plastic is being decomposed. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
32. Living Fungi in an Opencast Limestone Mine: Who Are They and What Can They Do?
- Author
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Sansupa, Chakriya, Purahong, Witoon, Nawaz, Ali, Wubet, Tesfaye, Suwannarach, Nakarin, Chantawannakul, Panuwan, Chairuangsri, Sutthathorn, and Disayathanoowat, Terd
- Subjects
- *
FUNGAL communities , *LIMESTONE quarries & quarrying , *LIMESTONE , *FUNGI , *SOIL enzymology , *MODULAR construction - Abstract
Opencast limestone mines or limestone quarries are considered challenging ecosystems for soil fungi as they are highly degraded land with specific conditions, including high temperature, prolonged sunlight exposure, and a lack of organic matter, moisture, and nutrients in soil. In such ecosystems, certain fungi can survive and have a crucial function in maintaining soil ecosystem functions. Unfortunately, we know very little about taxonomic diversity, potential functions, and the ecology of such fungi, especially for a limestone quarry in a tropical region. Here, we characterized and compared the living soil fungal communities in an opencast limestone mine, including mining site and its associated rehabilitation site (9 months post-rehabilitation), with the soil fungal community in a reference forest, using the amplicon sequencing of enrichment culture. Our results showed that living fungal richness in the quarry areas was significantly lower than that in the reference forest, and their community compositions were also significantly different. Living fungi in the mining sites mostly comprised of Ascomycota (Eurotiomycetes and Sordariomycetes) with strongly declined abundance or absence of Basidiomycota and Mucoromycota. After nine months of rehabilitation, certain taxa were introduced, such as Hypoxylon spp. and Phellinus noxius, though this change did not significantly differentiate fungal community composition between the mining and rehabilitation plots. The majority of fungi in these plots are classified as saprotrophs, which potentially produce all fifteen soil enzymes used as soil health indicators. Network analysis, which was analyzed to show insight into complex structures of living fungal community in the limestone quarry, showed a clear modular structure that was significantly impacted by different soil properties. Furthermore, this study suggests potential taxa that could be useful for future rehabilitation. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
33. View from the Top: Insights into the Diversity and Community Assembly of Ectomycorrhizal and Saprotrophic Fungi along an Altitudinal Gradient in Chinese Boreal Larix gmelinii -Dominated Forests.
- Author
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Guo, Yi, Ji, Li, Wang, Mingwei, Shan, Chengfeng, Shen, Fangyuan, Yang, Yuchun, He, Gongxiu, Purahong, Witoon, and Yang, Lixue
- Subjects
ECTOMYCORRHIZAL fungi ,COMMUNITIES ,FUNGAL communities ,LARCHES ,SOIL depth ,SOIL sampling ,MOUNTAIN soils ,FOREST soils - Abstract
The altitudinal patterns of soil fungi have attracted considerable attention; however, few studies have investigated the diversity and community assembly of fungal functional guilds along an altitudinal gradient. Here, we explored ectomycorrhizal (EcM) and saprotrophic (SAP) fungal diversity and community assembly along a 470 m vertical gradient (ranging from 830 to 1300 m) on Oakley Mountain, sampling bulk soils in the 0–10 cm and 10–20 cm soil layers of Larix gmelinii-dominated forests. Illumina MiSeq sequencing of the ITS genes was employed to explore the fungal community composition and diversity. The relative abundance of EcM and SAP fungi showed a divergent pattern along an altitudinal gradient, while we observed a consistent altitudinal tendency for EcM and SAP fungal diversity and community assembly. The diversity of both fungal guilds increased with increasing altitude. Altitude and soil moisture were the key factors affecting the community composition of both fungal guilds. In addition, the plant community composition significantly affected the EcM fungal community composition, whereas the dissolved organic nitrogen and ammonium nitrogen contents were the driving factors of SAP fungal community. Despite the effects of vegetation and soil factors, EcM and SAP fungal communities were mainly governed by stochastic processes (especially drift) at different altitudes and soil depths. These results shed new light on the ecology of different fungal functional guilds along an altitudinal gradient, which will provide a deeper understanding of the biogeography of soil fungi. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
34. More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing.
- Author
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Tanunchai, Benjawan, Schroeter, Simon Andreas, Li Ji, Wahdan, Sara Fareed Mohamed, Hossen, Shakhawat, Lehnert, Ann-Sophie, Grünberg, Hagen, Gleixner, Gerd, Buscot, François, Schulze, Ernst-Detlef, Noll, Matthias, and Purahong, Witoon
- Subjects
NUCLEOTIDE sequencing ,FUNGAL communities ,FOREST biodiversity ,SPECIES ,FRUITING bodies (Fungi) ,NORWAY spruce ,EPIPHYTIC lichens ,BIODIVERSITY - Abstract
Currently, lichen surveys are generally based on the examination of fruiting bodies. Lichens in the mycelial stage, in spores, or awaiting conditions for fruiting body formation are usually overlooked, even though they are important for maintaining biodiversity and ecosystem functions. This study aimed to explore the lichenized fungal community composition and richness associated with leaves and needles of 12 temperate tree species using Illumina MiSeq-based amplicon sequencing of the internal transcribed spacer (ITS) 2 region. Picea abies harbored the highest richness and number of lichenized fungal species. We found that the lichenized fungus Physcia adscendens dominated the leaves and needles of the most temperate tree species. Eleven lichenized fungal species detected in this study were recorded for the first time on leaves and needles. In addition, we identified Athallia cerinella, Fellhanera bouteillei, and Melanohalea exasperata that are on the German national red lists. Lichenized fungal richness was higher in conifer compared to broadleaf trees. Overall, tree species (within coniferous trees) and tree types (broadleaved vs. coniferous trees) harbored significantly different lichenized fungal community compositions pointing out the importance of host species. Diversity and community composition patterns of lichenized fungi were correlated mainly with tree species. Our study demonstrates that the diversity of foliicolous lichens associated with leaves and needles of 12 temperate tree species can be appropriately analyzed and functionally assigned using the ITS-based high-throughput sequencing. We highlighted the importance of conifers for maintaining the biodiversity of foliicolous lichens. Based on the discovery of many red list lichens, our methodological approach and results are important contributions to subsequent actions in the bio-conversation approaches. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
35. Targeting the Active Rhizosphere Microbiome of Trifolium pratense in Grassland Evidences a Stronger-Than-Expected Belowground Biodiversity-Ecosystem Functioning Link
- Author
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Wahdan, Sara Fareed Mohamed, Heintz-Buschart, Anna, Sansupa, Chakriya, Tanunchai, Benjawan, Wu, Yu-Ting, Schädler, Martin, Noll, Matthias, Purahong, Witoon, and Buscot, François
- Subjects
active microbiome, rhizosphere, biodiversity-ecosystem functioning, GCEF, BrdU ,ddc:570 - Abstract
The relationship between biodiversity and ecosystem functioning (BEF) is a central issue in soil and microbial ecology. To date, most belowground BEF studies focus on the diversity of microbes analyzed by barcoding on total DNA, which targets both active and inactive microbes. This approach creates a bias as it mixes the part of the microbiome currently steering processes that provide actual ecosystem functions with the part not directly involved. Using experimental extensive grasslands under current and future climate, we used the bromodeoxyuridine (BrdU) immunocapture technique combined with pair-end Illumina sequencing to characterize both total and active microbiomes (including both bacteria and fungi) in the rhizosphere of Trifolium pratense. Rhizosphere function was assessed by measuring the activity of three microbial extracellular enzymes (β-glucosidase, N-acetyl-glucosaminidase, and acid phosphatase), which play central roles in the C, N, and P acquisition. We showed that the richness of overall and specific functional groups of active microbes in rhizosphere soil significantly correlated with the measured enzyme activities, while total microbial richness did not. Active microbes of the rhizosphere represented 42.8 and 32.1% of the total bacterial and fungal taxa, respectively, and were taxonomically and functionally diverse. Nitrogen fixing bacteria were highly active in this system with 71% of the total operational taxonomic units (OTUs) assigned to this group detected as active. We found the total and active microbiomes to display different responses to variations in soil physicochemical factors in the grassland, but with some degree of resistance to a manipulation mimicking future climate. Our findings provide critical insights into the role of active microbes in defining soil ecosystem functions in a grassland ecosystem. We demonstrate that the relationship between biodiversity-ecosystem functioning in soil may be stronger than previously thought.
- Published
- 2021
36. Links among Microbial Communities, Soil Properties and Functions: Are Fungi the Sole Players in Decomposition of Bio-Based and Biodegradable Plastic?
- Author
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Guliyev, Vusal, Tanunchai, Benjawan, Noll, Matthias, Buscot, François, Purahong, Witoon, and Blagodatskaya, Evgenia
- Subjects
BIODEGRADABLE plastics ,MICROBIAL communities ,ACID phosphatase ,SOILS ,PHYTOPATHOGENIC microorganisms ,SOIL composition - Abstract
The incomplete degradation of bio-based and biodegradable plastics (BBPs) in soils causes multiple threats to soil quality, human health, and food security. Plastic residuals can interact with soil microbial communities. We aimed to link the structure and enzyme-mediated functional traits of a microbial community composition that were present during poly (butylene succinate-co-butylene adipate (PBSA) decomposition in soil with (PSN) and without (PS) the addition of nitrogen fertilizer ((NH
4 )2 SO4 ). We identified bacterial (Achromobacter, Luteimonas, Rhodanobacter, and Lysobacter) and fungal (Fusarium, Chaetomium, Clonostachys, Fusicolla, and Acremonium) taxa that were linked to the activities of ß-glucosidase, chitinase, phosphatase, and lipase in plastic-amended soils. Fungal biomass increased by 1.7 and 4 times in PS and PSN treatment, respectively, as compared to non-plastic amended soil. PBSA significantly changed the relationships between soil properties (C: N ratio, TN, and pH) and microbial community structure; however, the relationships between fungal biomass and soil enzyme activities remained constant. PBSA significantly altered the relationship between fungal biomass and acid phosphatase. We demonstrated that although the soil functions related to nutrient cycling were not negatively affected in PSN treatment, potential negative effects are reasoned by the enrichment of plant pathogens. We concluded that in comparison to fungi, the bacteria demonstrated a broader functional spectrum in the BBP degradation process. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
37. Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay.
- Author
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Purahong, Witoon, Tanunchai, Benjawan, Muszynski, Sarah, Maurer, Florian, Wahdan, Sara Fareed Mohamed, Malter, Jonas, Buscot, François, and Noll, Matthias
- Subjects
- *
BACTERIAL communities , *WOOD-decaying fungi , *WOOD decay , *ISOTOPIC signatures , *FUNGAL communities , *HYDROLASES - Abstract
Microbial community members are the primary microbial colonizers and active decomposers of deadwood. This study placed sterilized standardized beech and spruce sapwood specimens on the forest ground of 8 beech- and 8 spruce-dominated forest sites. After 370 days, specimens were assessed for mass loss, nitrogen (N) content and 15N isotopic signature, hydrolytic and lignin-modifying enzyme activities. Each specimen was incubated with bromodeoxyuridine (BrdU) to label metabolically active fungal and bacterial community members, which were assessed using amplicon sequencing. Fungal saprotrophs colonized the deadwood accompanied by a distinct bacterial community that was capable of cellulose degradation, aromatic depolymerization, and N2 fixation. The latter were governed by the genus Sphingomonas, which was co-present with the majority of saprotrophic fungi regardless of whether beech or spruce specimens were decayed. Moreover, the richness of the diazotrophic Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium group was significantly correlated with mass loss, N content and 15N isotopic signature. By contrast, presence of obligate predator Bdellovibrio spp. shifted bacterial community composition and were linked to decreased beech deadwood decay rates. Our study provides the first account of the composition and function of metabolically active wood-colonizing bacterial and fungal communities, highlighting cross-kingdom interactions during the early and intermediate stages of wood decay. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
38. Biodiversity across trophic levels drives multifunctionality in highly diverse forests
- Author
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Schuldt, Andreas, Assmann, Thorsten, Brezzi, Matteo, Buscot, François, Eichenberg, David, Gutknecht, Jessica, Härdtle, Werner, He, Jin-Sheng, Klein, Alexandra-Maria, Kühn, Peter, Liu, Xiaojuan, Ma, Keping, Niklaus, Pascal A., Pietsch, Katherina A., Purahong, Witoon, Scherer-Lorenzen, Michael, Schmid, Bernhard, Scholten, Thomas, Staab, Michael, Tang, Zhiyao, Trogisch, Stefan, von Oheimb, Goddert, Wirth, Christian, Wubet, Tesfaye, Zhu, Chao-Dong, and Bruelheide, Helge
- Subjects
Ecology ,Science ,Ecosystem services ,lcsh:Q ,Biodiversity ,Forest ecology ,Tropical ecology ,lcsh:Science ,Article ,Environmental planning - Abstract
Human-induced biodiversity change impairs ecosystem functions crucial to human well-being. However, the consequences of this change for ecosystem multifunctionality are poorly understood beyond effects of plant species loss, particularly in regions with high biodiversity across trophic levels. Here we adopt a multitrophic perspective to analyze how biodiversity affects multifunctionality in biodiverse subtropical forests. We consider 22 independent measurements of nine ecosystem functions central to energy and nutrient flow across trophic levels. We find that individual functions and multifunctionality are more strongly affected by the diversity of heterotrophs promoting decomposition and nutrient cycling, and by plant functional-trait diversity and composition, than by tree species richness. Moreover, cascading effects of higher trophic-level diversity on functions originating from lower trophic-level processes highlight that multitrophic biodiversity is key to understanding drivers of multifunctionality. A broader perspective on biodiversity-multifunctionality relationships is crucial for sustainable ecosystem management in light of non-random species loss and intensified biotic disturbances under future environmental change., Biodiversity change can impact ecosystem functioning, though this is primarily studied at lower trophic levels. Here, Schuldt et al. find that biodiversity components other than tree species richness are particularly important, and higher trophic level diversity plays a role in multifunctionality.
- Published
- 2018
39. Nitrogen fixing bacteria facilitate microbial biodegradation of a bio-based and biodegradable plastic in soils under ambient and future climatic conditions.
- Author
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Tanunchai, Benjawan, Kalkhof, Stefan, Guliyev, Vusal, Wahdan, Sara Fareed Mohamed, Krstic, Dennis, Schädler, Martin, Geissler, Andreas, Glaser, Bruno, Buscot, François, Blagodatskaya, Evgenia, Noll, Matthias, and Purahong, Witoon
- Abstract
We discovered a biological mechanism supporting microbial degradation of bio-based poly(butylene succinate-co-adipate) (PBSA) plastic in soils under ambient and future climates. Here, we show that nitrogen-fixing bacteria facilitate the microbial degradation of PBSA by enhancing fungal abundance, accelerating plastic-degrading enzyme activities, and shaping/interacting with plastic-degrading fungal communities. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
40. Organic agricultural practice enhances arbuscular mycorrhizal symbiosis in correspondence to soil warming and altered precipitation patterns.
- Author
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Wahdan, Sara Fareed Mohamed, Reitz, Thomas, Heintz‐Buschart, Anna, Schädler, Martin, Roscher, Christiane, Breitkreuz, Claudia, Schnabel, Beatrix, Purahong, Witoon, and Buscot, François
- Subjects
SOIL heating ,VESICULAR-arbuscular mycorrhizas ,SOIL microbiology ,AGRICULTURAL productivity ,WHEAT ,SOIL composition ,ORGANIC farming - Abstract
Summary: Climate and agricultural practice interact to influence both crop production and soil microbes in agroecosystems. Here, we carried out a unique experiment in Central Germany to simultaneously investigate the effects of climates (ambient climate vs. future climate expected in 50–70 years), agricultural practices (conventional vs. organic farming), and their interaction on arbuscular mycorrhizal fungi (AMF) inside wheat (Triticum aestivum L.) roots. AMF communities were characterized using Illumina sequencing of 18S rRNA gene amplicons. We showed that climatic conditions and agricultural practices significantly altered total AMF community composition. Conventional farming significantly affected the AMF community and caused a decline in AMF richness. Factors shaping AMF community composition and richness at family level differed greatly among Glomeraceae, Gigasporaceae and Diversisporaceae. An interactive impact of climate and agricultural practices was detected in the community composition of Diversisporaceae. Organic farming mitigated the negative effect of future climate and promoted total AMF and Gigasporaceae richness. AMF richness was significantly linked with nutrient content of wheat grains under both agricultural practices. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
41. Characterization of the Castanopsis carlesii Deadwood Mycobiome by Pacbio Sequencing of the Full-Length Fungal Nuclear Ribosomal Internal Transcribed Spacer (ITS)
- Author
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Purahong, Witoon, Mapook, Ausana, Wu, Yu-Ting, and Chen, Chaur-Tzuhn
- Subjects
fungal diversity ,wood-inhabiting fungi ,NGS ,long-read sequencing ,species identification ,Methods ,Taiwan ,fungal community ,Microbiology - Abstract
Short-read next generation sequencing (NGS) platforms can easily and quickly generate thousands to hundreds of thousands of sequences per sample. However, the limited length of these sequences can cause problems during fungal taxonomic identification. Here we validate the use of Pacbio sequencing, a long-read NGS method, for characterizing the fungal community (mycobiome) of Castanopsis carlesii deadwood. We report the successful use of Pacbio sequencing to generate long-read sequences of the full-length (500–780 bp) fungal ITS regions of the C. carlesii mycobiome. Our results show that the studied deadwood mycobiome is taxonomically and functionally diverse, with an average of 85 fungal OTUs representing five functional groups (animal endosymbionts, endophytes, mycoparasites, plant pathogens, and saprotrophs). Based on relative abundance data, Basidiomycota were the most frequently detected phyla (50% of total sequences), followed by unidentified phyla, and Ascomycota. However, based on presence/absence data, the most OTU-rich phyla were Ascomycota (58% of total OTUs, 72 OTUs) followed by Basidiomycota and unidentified phyla. The majority of fungal OTUs were identified as saprotrophs (70% of successfully function-assigned OTUs) followed by plant pathogens. Finally, we used phylogenetic analysis based on the full-length ITS sequences to confirm the species identification of 14/36 OTUs with high bootstrap support (99–100%). Based on the numbers of sequence reads obtained per sample, which ranged from 3,047 to 13,463, we conclude that Pacbio sequencing can be a powerful tool for characterizing moderate- and possibly high-complexity fungal communities.
- Published
- 2019
42. Deciphering Trifolium pratense L. holobiont reveals a microbiome resilient to future climate changes.
- Author
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Wahdan, Sara Fareed Mohamed, Tanunchai, Benjawan, Wu, Yu‐Ting, Sansupa, Chakriya, Schädler, Martin, Dawoud, Turki M., Buscot, François, and Purahong, Witoon
- Published
- 2021
- Full Text
- View/download PDF
43. Life on the Rocks: First Insights Into the Microbiota of the Threatened Aquatic Rheophyte Hanseniella heterophylla.
- Author
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Purahong, Witoon, Hossen, Shakhawat, Nawaz, Ali, Sadubsarn, Dolaya, Tanunchai, Benjawan, Dommert, Sven, Noll, Matthias, Ampornpan, La-aw, Werukamkul, Petcharat, and Wubet, Tesfaye
- Subjects
HUMAN microbiota ,BIOINDICATORS ,ECTOMYCORRHIZAL fungi ,PLANT indicators ,PLANT communities ,AQUATIC plants ,PATHOGENIC fungi - Abstract
Little is known about microbial communities of aquatic plants despite their crucial ecosystem function in aquatic ecosystems. Here, we analyzed the microbiota of an aquatic rheophyte, Hanseniella heterophylla , growing at three areas differing in their degree of anthropogenic disturbance in Thailand employing a metabarcoding approach. Our results show that diverse taxonomic and functional groups of microbes colonize H. heterophylla. Proteobacteria, Actinobacteria, Dothideomycetes, and Sordariomycetes form the backbone of the microbiota. Surprisingly, the beneficial microbes reported from plant microbiomes in terrestrial habitats, such as N-fixing bacteria and ectomycorrhizal fungi, were also frequently detected. We showed that biofilms for attachment of H. heterophylla plants to rocks may associate with diverse cyanobacteria (distributed in eight families, including Chroococcidiopsaceae, Coleofasciculaceae, Leptolyngbyaceae, Microcystaceae, Nostocaceae, Phormidiaceae, Synechococcaceae, and Xenococcaceae) and other rock biofilm-forming bacteria (mainly Acinetobacter , Pseudomonas , and Flavobacterium). We found distinct community compositions of both bacteria and fungi at high and low anthropogenic disturbance levels regardless of the study areas. In the highly disturbed area, we found strong enrichment of Gammaproteobacteria and Tremellomycetes coupled with significant decline of total bacterial OTU richness. Bacteria involved with sulfamethoxazole (antibiotic) degradation and human pathogenic fungi (Candida , Cryptococcus , Trichosporon , and Rhodotorula) were exclusively detected as indicator microorganisms in H. heterophylla microbiota growing in a highly disturbed area, which can pose a major threat to human health. We conclude that aquatic plant microbiota are sensitive to anthropogenic disturbance. Our results also unravel the potential use of this plant as biological indicators in remediation or treatment of such disturbed ecosystems. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
44. Soil bacterial communities and their associated functions for forest restoration on a limestone mine in northern Thailand.
- Author
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Sansupa, Chakriya, Purahong, Witoon, Wubet, Tesfaye, Tiansawat, Pimonrat, Pathom-Aree, Wasu, Teaumroong, Neung, Chantawannakul, Panuwan, Buscot, François, Elliott, Stephen, and Disayathanoowat, Terd
- Subjects
- *
FOREST restoration , *BACTERIAL communities , *FOREST soils , *LIMESTONE , *SOIL restoration , *SOIL composition , *SUBSOILS , *BACTERIAL diversity - Abstract
Opencast mining removes topsoil and associated bacterial communities that play crucial roles in soil ecosystem functioning. Understanding the community composition and functioning of these organisms may lead to improve mine-rehabilitation practices. We used a culture-dependent method, combined with Illumina sequencing, to compare the taxonomic richness and composition of living bacterial communities in opencast mine substrates and young mine-rehabilitation plots, with those of soil in adjacent remnant forest at a limestone mine in northern Thailand. We further investigated the effects of soil physico-chemical factors and ground-flora cover on the same. Although, loosened subsoil, brought in to initiate rehabilitation, improved water retention and facilitated plant re-establishment, it did not increase the population density of living microbes substantially within 9 months. Planted trees and sparse ground flora in young rehabilitation plots had not ameliorated the micro-habitat enough to change the taxonomic composition of the soil bacteria compared with non-rehabilitated mine sites. Viable microbes were significantly more abundant in forest soil than in mine substrates. The living bacterial community composition differed significantly, between the forest plots and both the mine and rehabilitation plots. Proteobacteria dominated in forest soil, whereas Firmicutes dominated in samples from both mine and rehabilitation plots. Although, several bacterial taxa could survive in the mine substrate, soil ecosystem functions were greatly reduced. Bacteria, capable of chitinolysis, aromatic compound degradation, ammonification and nitrate reduction were all absent or rare in the mine substrate. Functional redundancy of the bacterial communities in both mine substrate and young mine-rehabilitation soil was substantially reduced, compared with that of forest soil. Promoting the recovery of microbial biomass and functional diversity, early during mine rehabilitation, is recommended, to accelerate soil ecosystem restoration and support vegetation recovery. Moreover, if inoculation is included in mine rehabilitation programs, the genera: Bacillus, Streptomyces and Arthrobacter are likely to be of particular interest, since these genera can be cultivated easily and this study showed that they can survive under the extreme conditions that prevail on opencast mines. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
45. Can We Use Functional Annotation of Prokaryotic Taxa (FAPROTAX) to Assign the Ecological Functions of Soil Bacteria?
- Author
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Sansupa, Chakriya, Wahdan, Sara Fareed Mohamed, Hossen, Shakhawat, Disayathanoowat, Terd, Wubet, Tesfaye, and Purahong, Witoon
- Subjects
SOIL microbiology ,FOREST soils ,FORESTS & forestry ,BACTERIA classification ,SOIL sampling - Abstract
FAPROTAX is a promising tool for predicting ecological relevant functions of bacterial and archaeal taxa derived from 16S rRNA amplicon sequencing. The database was initially developed to predict the function of marine species using standard microbiological references. This study, however, has attempted to access the application of FAPROTAX in soil environments. We hypothesized that FAPROTAX was compatible with terrestrial ecosystems. The potential use of FAPROTAX to assign ecological functions of soil bacteria was investigated using meta-analysis and our newly designed experiments. Soil samples from two major terrestrial ecosystems, including agricultural land and forest, were collected. Bacterial taxonomy was analyzed using Illumina sequencing of the 16S rRNA gene and ecological functions of the soil bacteria were assigned by FAPROTAX. The presence of all functionally assigned OTUs (Operation Taxonomic Units) in soil were manually checked using peer-reviewed articles as well as standard microbiology books. Overall, we showed that sample source was not a predominant factor that limited the application of FAPROTAX, but poor taxonomic identification was. The proportion of assigned taxa between aquatic and non-aquatic ecosystems was not significantly different (p > 0.05). There were strong and significant correlations (σ = 0.90–0.95, p < 0.01) between the number of OTUs assigned to genus or order level and the number of functionally assigned OTUs. After manual verification, we found that more than 97% of the FAPROTAX assigned OTUs have previously been detected and potentially performed functions in agricultural and forest soils. We further provided information regarding taxa capable of N-fixation, P and K solubilization, which are three main important elements in soil systems and can be integrated with FAPROTAX to increase the proportion of functionally assigned OTUs. Consequently, we concluded that FAPROTAX can be used for a fast-functional screening or grouping of 16S derived bacterial data from terrestrial ecosystems and its performance could be enhanced through improving the taxonomic and functional reference databases. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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46. Characterization of Unexplored Deadwood Mycobiome in Highly Diverse Subtropical Forests Using Culture-independent Molecular Technique
- Author
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Purahong, Witoon, Pietsch, Katherina A., Lentendu, Guillaume, Schöps, Ricardo, Bruelheide, Helge, Wirth, Christian, Buscot, François, and Wubet, Tesfaye
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fungal diversity ,pyrosequencing ,abiotic factor ,biotic factor ,BEF China ,wood decomposition ,Microbiology ,ecosystem processes ,subtropical forest - Abstract
The deadwood mycobiome, also known as wood-inhabiting fungi (WIF), are among the key players in wood decomposition, having a large impact on nutrient cycling in forest soils. However, our knowledge of WIF richness and distribution patterns in different forest biomes is limited. Here, we used pyrotag sequencing of the fungal internal transcribed spacer (ITS2) region to characterize the deadwood mycobiome of two tree species with greatly different wood characteristics (Schima superba and Pinus massoniana) in a Chinese subtropical forest ecosystem. Specifically, we tested (i) the effects of tree species and wood quality properties on WIF OTU richness and community composition; (ii) the role of biotic and abiotic factors in shaping the WIF communities; and (iii) the relationship between WIF OTU richness, community composition and decomposition rates. Due to different wood chemical properties, we hypothesized that the WIF communities derived from the two tree species would be correlated differently with biotic and abiotic factors. Our results show that deadwood in subtropical forests harbors diverse fungal communities comprising six ecological functional groups. We found interesting colonization patterns for this subtropical biome, where Resinicium spp. were highly detected in both broadleaved and coniferous deadwood. In addition, the members of Xylariales were frequently found in Schima. The two deadwood species differed significantly in WIF OTU richness (Pinus > Schima) and community composition (P < 0.001). Variations in WIF community composition of both tree species were significantly explained by wood pH and ecological factors (biotic: deadwood species, basal area and abiotic: soil pH), but the WIF communities derived from each tree species correlated differently with abiotic factors. Interestingly, we found that deadwood decomposition rate significantly correlated with WIF communities and negatively correlated with WIF OTU richness. We conclude that the pattern of WIF OTU richness and community composition are controlled by multiple interacting biotic and abiotic factors. Overall, our study provides an in-depth picture of the deadwood mycobiome in this subtropical forest. Furthermore, by comparing our results to results from temperate and boreal forests we contribute to a better understanding of patterns of WIF communities across different biomes and geographic locations.
- Published
- 2017
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47. Superimposed Pristine Limestone Aquifers with Marked Hydrochemical Differences Exhibit Distinct Fungal Communities
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Nawaz, Ali, Purahong, Witoon, Lehmann, Robert, Herrmann, Martina, Küsel, Kirsten, Totsche, Kai U., Buscot, François, and Wubet, Tesfaye
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Microbiology (medical) ,ITS gene ,pyrosequencing ,Hainich CZE ,karst ,fungal communities ,Microbiology ,Upper Muschelkalk groundwater - Abstract
Fungi are one important group of eukaryotic microorganisms in a diverse range of ecosystems, but their diversity in groundwater ecosystems is largely unknown. We used DNA-based pyro-tag sequencing of the fungal internal transcribed spacer (ITS) rDNA gene to investigate the presence and community structure of fungi at different sampling sites of two superimposed limestone aquifers ranging from 8.5 to 84 m depth in the newly established Hainich Critical Zone Exploratory (Hainich CZE). We detected a diversity of fungal OTUs in groundwater samples of all sampling sites. The relative percentage abundance of Basidiomycota was higher in the upper aquifer assemblage, whilst Ascomycota dominated the lower one. In parallel to differences in the hydrochemistry we found distinct fungal communities at all sampling sites. Classification into functional groups revealed an overwhelming majority of saprotrophs. Finding taxa common to all analyzed groundwater sites, point to a groundwater specific fungal microbiome. The presence of different functional groups and, in particular plant and cattle pathogens that are not typical of subsurface habitats, suggests links between the surface and subsurface biogeosphere due to rapid transportation across the fracture networks typical of karstic regions during recharge episodes. However, further studies including sampling series extended in both time and space are necessary to confirm this hypothesis.
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- 2016
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48. Molecular microbial diversity of deadwood and leaf litter in forests of different forest management intensity
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Purahong, Witoon, Schloter, Michael (Prof. Dr.), Weisser, Wolfgang (Prof. Dr.), and Buscot, Francois (Prof. Dr.)
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Biowissenschaften, Biologie ,ddc:570 - Abstract
The effects of forest management and conversion of forest types on microbial diversity and ecological functions and services are still unclear. Together, the investigations detailed in this dissertation help unravel the relationships between forest management and the structure, diversity and functions of fungal and/or bacterial communities involved in decomposition of deadwood and leaf litter, a key aspect of element cycling in forest ecosystems. Understanding these relationships provides a basis to evaluate ecosystem services provided by microbial communities and the impact of forest management on such services. Die Auswirkungen der Waldbewirtschaftung und der Umwandlung von Waldtypen auf die mikrobielle Diversität und ökologischen Funktionen und Dienste sind noch unklar. Die in dieser Dissertation beschriebenen Untersuchungen helfen die Beziehung zwischen Waldbewirtschaftung, der Struktur, Vielfalt und Funktionen der Pilz-und/oder Bakteriengemeinschaften in der Zersetzung von Totholz und Laub, einem wichtigen Aspekt der Stoffkreisläufe in Waldökosystemen, aufzuklären. Das Verständnis dieser Zusammenhänge stellt die Grundlage her, Ökosystemdienstleistungen von mikrobiellen Gemeinschaften und die Auswirkungen der Waldbewirtschaftung auf solche Dienstleistungen zu evaluieren.
- Published
- 2015
49. Application of next‐generation sequencing technologies to conservation of wood‐inhabiting fungi.
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Purahong, Witoon, Wubet, Tesfaye, Krüger, Dirk, and Buscot, François
- Subjects
- *
MICROBIAL communities , *FUNGI conservation , *FOREST management , *TEMPERATE forests , *SPECIES diversity - Abstract
Next‐generation sequencing (NGS) has significantly increased knowledge of microbial communities and their distribution. However, it is still not common to apply NGS technology to microbial conservation. We sought to use NGS technologies to evaluate conservation strategies for wood‐inhabiting fungi. Evaluating a deadwood experiment 3 years after it was established, we specifically examined which tree species combinations promoted the highest richness of wood‐inhabiting fungi. Deadwood enrichment was an effective strategy and logs of 6 tree species, either those with the highest wood‐inhabiting fungal α and γ diversity or those with the highest β diversity, maintained >1,000 operational taxonomic units (OTUs) spread over a wide range of taxonomic groups. In comparison, a conservation strategy based only on the results of sporocarp surveys yielded 591 OTUs. This result highlights the need to use NGS approaches to inform microbial conservation strategies. We also determined that 5 tree species with the highest saproxylic beetle γ diversity simultaneously conserved wood‐inhabiting fungi. Apart from deadwood volume, we suggest data on deadwood quality and species also be included as indicators, especially for wood‐inhabiting fungal diversity, and incorporated quickly in forest assessment and monitoring systems in Central Europe. Article impact statement: Enrichment of the species diversity of deadwood is an effective strategy for wood‐inhabiting fungal conservation in temperate forests. [ABSTRACT FROM AUTHOR]
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- 2019
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50. Wood decomposition is more strongly controlled by temperature than by tree species and decomposer diversity in highly species rich subtropical forests.
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Pietsch, Katherina A., Eichenberg, David, Nadrowski, Karin, Bauhus, Jürgen, Buscot, François, Purahong, Witoon, Wipfler, Benjamin, Wubet, Tesfaye, Yu, Mingjian, and Wirth, Christian
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INVERTEBRATE diversity ,TROPICAL plants ,BIODIVERSITY ,BIOGEOCHEMICAL cycles ,WOOD-decaying fungi - Abstract
While the number of studies on the role of biodiversity on ecosystem functioning is steadily increasing, a key component of biogeochemical cycling in forests, dead wood decay, has been largely neglected. It remains widely unknown whether and how dead wood decay is affected by diversity loss in forests. We studied the hierarchical effects of tree species diversity on wood decay rates in a subtropical forest landscape in southeast China via its influence on fungal OTU richness and invertebrate diversity using piecewise structural equation models. The experiment was conducted in natural forest plots that span a wide gradient of tree species diversity embedded in a heterogeneous topography. To account for interactions between macro‐invertebrates and fungi, that potentially modify the influence of tree biodiversity and climate on dead wood decay, we compared a macro‐invertebrate exclusion treatment with a control treatment that allowed access to all types of decomposers. Diversity effects of trees on wood decay rates were mostly negative and mediated by the diversity of macro‐invertebrates. However, the effects of tree species diversity or fungal OTU richness and macro‐invertebrate diversity on wood decay rates were comparatively weak. Temperature affected decay rates positively and had the strongest influence in all treatments. While the exclusion of macro‐invertebrates did not lead to a reduction of wood decay rates, our results suggest that they may however have a mediating role in the process. In the presence of invertebrates the predictability of wood decay rates was higher and we observed a tendency of a stronger temperature control. Our results suggest that there is evidence for diversity effects on wood decomposition, but the temperature control is still more important. Thus, an increase in mean annual temperature will increase carbon and nutrient turnover through wood decomposition in subtropical forest irrespective of biotic composition. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
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