Search

Carica papaya seeds were used in this research for the synthesis of AgNPs. The production of silver nanoparticles was characterized using UV–Vis spectroscopy, FTIR, XRD and SEM-EDX analysis. The antibacterial activity of the prepared AgNPs was evaluated by the Kirby–Bauer disc diffusion method with the antibacterial activity of different compounds against Bacillus aerogenes. UV–Vis spectral peaks are found at 8–18 nm. SEM and TEM analysis revealed several spherical structures between 10 nm and 50 nm. XRD analysis also confirmed its 50 nm size. Experimental data revealed that AgNPs inhibited bacterial growth. This study shows that papaya seed extract contributes to the bioreduction and stabilization of the synthesized silver nanoparticles. [ABSTRACT FROM AUTHOR]

Nonwoven fabrics are the primary source of disposable medical clothing, which cause a huge medical waste. One possible way to reuse this disposable clothing is through antibacterial treatment. Among various metal nanoparticles, silver nanoparticles (AgNPs) are preferable regarding ecology and human health. Green synthesis is an ecological alternative to synthesize AgNPs from silver nitrate (AgNO3) solution. The silver nanoparticles (AgNPs) were successfully synthesized from Calendula arvensis extract and AgNO3 at a certain temperature and incorporated into the fabric by padding and in-situ method. The pretreatments applied to the nonwoven fabric were found very important in terms of providing antibacterial activity. AgNPs applied according to the in situ method could provide 99% antibacterial activity on nonwoven surface fabrics. The samples treated by AgNPs with the in situ method performed better for both particle filtration efficiency (PFE) and bacterial filtration efficiency (BFE) than the samples treated with the padding method. [ABSTRACT FROM AUTHOR]

Electrospinning has garnered much attention for skin renewal, emphasizing skin's pivotal role as a primary protective layer and the susceptibility to loss caused by burns. The research aimed to develop a multipurpose wound dressing that safeguard injuries and facilitates the renewal of dermal tissues. Two-layer nanofibers were prepared using polyvinyl alcohol–chitosan–gelatin/polyacrylonitrile (PVA–CS–Gel/PAN), containing mupirocin (Mu 3% w/w) in underlying layer and silver nanoparticles (AgNps) in the upper layer with varying concentrations. AgNps were synthesized from Capsella bursa-pastoris extract by the green method and characterized using XRD, SEM, FTIR, and UV techniques. Then, PVA–CS–Gel/PAN solutions with AgNps and Mu were electrospun into multilayer dressings. The effectiveness of the nanofibers was evaluated through in vitro and in vivo tests. The study examined the nanofibers containing spherical AgNps with an average diameter of 72.57 nm and a negative surface charge (− 12 mv). They had uniform and smooth surfaces with a diameter range of 476.31 to 926.04 nm. According to swelling and contact angle results, hydrophilicity of samples had a direct relation with water absorption. Controlled drug release within 72 h followed Higuchi or first-order profiles. MTT and antibacterial analyses indicated that optimized nanofibers (Mu/1% AgNps) had suitable biocompatibility and synergistic potential against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. Additionally, an in vivo test on rats with second-degree burns after 28 days demonstrated a 98.97% improvement in revival, outperforming its commercial counterparts. Consequently, the results position the designed composite nanofiber as a promising candidate for wound healing applications, aiding in enhancing skin regeneration. [ABSTRACT FROM AUTHOR]

The present study focused on evaluating the antibacterial properties, radical scavenging, and photocatalytic activities of Centaurea behen-mediated silver nanoparticles (Cb-AgNPs). The formation of Cb-AgNPs was approved by UV–Vis spectrometry, Fourier-transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. The results showed that the obtained AgNPs have a maximum absorbance peak at 450 nm with spherical morphology and an average size of 13.03 ± 5.8 nm. The catalytic activity of the Cb-AgNPs was investigated using Safranin O (SO) solution as a cationic dye model. The Cb-AgNPs performed well in the removal of SO. The coupled physical adsorption/photocatalysis reaction calculated about 68% and 98% degradation of SO dye under solar irradiation. The Cb-AgNPs inhibited the growth of gram-negative or positive bacteria strains and had excellent DPPH radicals scavenging ability (100% in a concentration of 200 µg/ml) as well as a good effect on reducing coagulation time (at concentrations of 200 and 500 µg/mL reduced clotting time up to 3 min). Considering the fact that green synthesized Cb-AgNPs have antioxidant and antibacterial properties and have a good ability to reduce coagulation time, they can be used in wound dressings. As well as these NPs with good photocatalytic activity can be a suitable option for degrading organic pollutants. [ABSTRACT FROM AUTHOR]

The adoption of green chemistry protocols in nanoparticle (NP) synthesis has exhibited substantial potential and is presently a central focus in research for generating versatile NPs applicable across a broad spectrum of applications. In this scientific contribution, we, for the first time, examined the ability of Aconitum Laeve (A. Laeve) crude extract to synthesize silver and gold nanoparticles (AgNPs@AL; AuNP@AL) and explored their potential applications in biological activities and the catalytic degradation of environmental pollutants. The synthesized NPs exhibited a distinctive surface plasmon resonance pattern, a spherical morphology with approximate sizes of 5–10 nm (TEM imaging), a crystalline architecture (XRD analysis), and potential functional groups identified by FTIR spectroscopy. The antibacterial activity was demonstrated by inhibition zones that measured 16 and 14 mm for the AgNPs@AL and AuNP@AL at a concentration of 80 µg/mL against Staphylococcus aureus and 14 and 12 mm against Escherichia coli, respectively. The antioxidant potential of the synthesized NPs was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-Phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-Oxide (PTIO), and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. Our findings suggest that the AuNP@AL effectively countered the tested radicals considerably, displaying IC50 values of 115.9, 103.54, and 180.85 µg/mL against DPPH, PTIO, and ABTS, respectively. In contrast, the AgNPs@AL showed IC50 values of 144.9, 116.36, and 95.39 µg/mL against the respective radicals. In addition, both the NPs presented significant effectiveness in the photocatalytic degradation of methylene blue and rhodamine B. The overall observations indicate that A. Laeve possesses a robust capability to synthesize spherical nanoparticles, exhibiting excellent dispersion and showcasing potential applications in both biological activities and environmental remediation. [ABSTRACT FROM AUTHOR]

A comparative study was applied to investigate the potential of Callistemon citrinus (bottlebrush) flower extract (BBE) and Punica granatum (pomegranate) peel extracts (PPE) for the sustainable synthesis of the silver nanoparticles, Ag-BBE and Ag-PPE, respectively. The synthesis process of Ag NPs using the selected extracts was applied under optimized conditions. Hence, the effect of the selected plant's type on the different characteristics of the synthesized green Ag NPs was investigated. The UV-Vis spectroscopy revealed the presence of the characteristic silver peaks at 419 and 433 nm of the Ag-BBE and Ag-PPE, respectively. The XRD spectra reported the fcc phase formation of Ag NPs. The TEM results highlighted the morphological features of the synthesized Ag NPs. with a size range of 20–70 nm, and with 10–30 nm for Ag-BBE and Ag-PPE, correspondingly. The Raman spectra revealed characteristic silver bands in the Ag-PPE and reflected some bands related to the natural extract in the Ag-BBE sample. The antimicrobial activity and statistical analysis investigation were conducted against four selected oral pathogens (Staphylococcus aureus (SA), Candida albicans (CA), Staphylococcus epidermidis (S. epi), and Enterococcus faecalis (EF)). Both tested extracts, BBE, and PPE, revealed potential effectivity as reducing and capping agents for Ag NP green synthesis. However, the synthesized NPs demonstrated different features, depending on the used extract, reflecting the influence of the plant's biomolecules on the nanoparticles' properties. [ABSTRACT FROM AUTHOR]

Metal nanoparticles (MNPs) have gained significant attention due to their multifunctional properties and broad applications in various fields. In this study, we present a novel and sustainable approach for the synthesis of silver nanoparticles (AgNPs) using waste materials, dried banana pith extract (BPE) and rice water. The synthesised AgNPs exhibit a size of 43 nm and zeta potential of −24.1 mV. A maximum temperature rise of 4°C with an efficiency of 41% is achieved within a short time by the photothermal activity. The qualitative colorimetric detection of Fe3+ and its quantitative spectroscopic detection are achieved in a linear detection range of 20–80 μM and with a limit of detection of 8.5 μM. The sensing mechanism is explained in terms of cooperative binding of Fe3+ ions to BPE-AgNPs. A homogeneous, flexible and stable AgNP-rice starch gel is formed. The work offers environmentally conscious nanomaterials for diverse applications. [ABSTRACT FROM AUTHOR]

Herein, a convenient and environment-friendly reduction strategy was employed to attach silver nanoparticles onto ceria nanorods (AgCe). Nanorods were synthesised through a green chemistry approach with varied silver contents and Citrus maxima peel extract as a reducing agent. The resulting AgCe nanocomposites were evaluated for their efficacy in reducing methyl orange (MO) at ambient temperature by employing NaBH4 as a protonating agent. The as-prepared samples were characterised by X-ray diffraction, Fourier transform infrared spectroscopy, X-ray energy-dispersive spectroscopy, Raman spectroscopy, Brunauer–Emmett–Teller method, and high-resolution transmission electron microscopy. The as-prepared sample exhibited outstanding activity on the catalytic reduction of MO, and the synergistic effect between Ag nanoparticles and CeO2 nanorods was proved. Silver nanoparticles were formed and dispersed well on the surface of ceria nanorods. Based on the UV–Vis results, the activity of the samples with silver was superior to that of pure ceria. The 0.2AgCe sample was the most effective when the catalyst concentration was 0.08 g/L, and the molar ratio between MO and NaBH4 was 1/200. The entire catalytic reduction was thought to follow pseudo-first-order kinetics. Hence, Ag nanoparticles decorated on CeO2 efficiently offered excellent catalytic efficiency and stability in MO reduction and that the catalyst was suitable for practical applications, such as wastewater treatment. [ABSTRACT FROM AUTHOR]

Pomegranate waste poses an environmental challenge in Arequipa. Simultaneously, interest in sustainable materials like natural rubber latex (NRL) is growing, with Peruvian communities offering a promising source. This study explores the green synthesis of silver nanoparticles (AgNPs) using pomegranate peel extract and their incorporation into NRL nanofibers for enhanced functionalities. An eco-friendly process utilized silver nitrate and pomegranate peel extract as a reducing and capping agent to synthesize AgNPs. The resulting AgNPs and NRL/AgNPs nanofibers were characterized using imaging and spectroscopic techniques such as UV-vis, TGA, FTIR, XRD, Raman, SEM, and DLS. Green-synthesized AgNPs were spherical and crystalline, with an average diameter of 59 nm. They showed activity against K. pneumoniae, E. coli, B. cereus, and S. aureus (IC50: 51.32, 4.87, 27.72, and 69.72 µg/mL, respectively). NRL and NRL/AgNPs nanofibers (300–373 nm diameter) were successfully fabricated. The composite nanofibers exhibited antibacterial activity against K. pneumoniae and B. cereus. This study presents a sustainable approach by utilizing pomegranate waste for AgNP synthesis and NRL sourced from Peruvian communities. Integrating AgNPs into NRL nanofibers produced composites with antimicrobial properties. This work has potential applications in smart textiles, biomedical textiles, and filtration materials where sustainability and antimicrobial functionality are crucial. [ABSTRACT FROM AUTHOR]

AbstractFor the first time, methanolic leaf extract of the hydrophyte Persicaria senegalensis L. was evaluated for its ability to silver nanoparticles (AgNPs) biosynthesis. The effect of stirring time and plant extract concentrations was studied. X-ray diffraction (XRD) and scanning electron microscope (SEM) were used for characterization of AgNPs. XRD confirmed the AgNPs formation with crystallite size in the range 23–71 nm. The crystallite size increased with increasing both stirring time and plant extract concentration. Antioxidants, including secondary metabolites such as flavonoids and phenols are substances that inhibit oxidative damage to the cell organisms by scavenging the free radicals and thus reducing the hazard of diseases. The scavenging and antioxidant activities of the plant leaf extract and AgNPs were evaluated and exhibited potent antioxidant activity. The total antioxidant capacity (TAC) of plant leaf extract and AgNPs was 52.41 and 10.13 µg AA equivalent/mg DW, respectively. Total phenolics (TPs) and total flavonoids (TFs), revealed high contents equal to 154.83 µg gallic acid equivalent and 100.05 µg quercetin equivalent/mg DW plant extract, respectively. [ABSTRACT FROM AUTHOR]

This work aims to analyze the influence of selected plants on the synthesis of silver nanoparticles (AgNPs). Six plants were chosen for the experiment, from which extracts were prepared: maclura fruit, spruce and ginkgo needles, green algae (Ch. kessleri), and mushrooms, namely Collybia nuda, and Macrolepiota procera. The composition of the extracts and colloids after preparation of the nanoparticles was analyzed using FTIR analysis. The composition of the extracts affected not only the rate of the synthesis but also the shape of the nanoparticles. TEM analysis confirmed the synthesis of mainly spherical nanoparticles (size range: 10–25 nm). However, triangular prisms and polyhedral nanoparticles synthesized by the extracts containing mainly flavonoids, terpenes, and phenols (the main compounds of resins) were also confirmed. EDS analysis was used to analyze the composition of the nanoparticles. It was proven that by choosing the right plant extract and using the appropriate technology with extract treatment, it is possible to prepare nanoparticles of different shapes. [ABSTRACT FROM AUTHOR]

Metal nanoparticle synthesis via environmentally friendly methods is gaining interest for their potential advantages over conventional physico-chemical approaches. Herein, we propose a robust green synthesis route for lignin-modified silver nanoparticles, utilizing the recovery of lignin as a renewable raw material and exploring its application in valuable areas. Through a systematic approach combining UV-Vis spectroscopy with AAS and DLS, we identified repeatable and scalable reaction conditions in an aqueous solution at pH 11 for homogeneous silver nanoparticles with high uniformity. The TEM median sizes ranged from 12 to 15 nm with circularity between 0.985 and 0.993. The silver nanoparticles yield exceeded 0.010 mol L−1, comparable with traditional physico-chemical methods, with a minimal loss of silver precursor ranging between 0.5 and 3.9%. Characterization by XRD and XPS revealed the presence of Ag-O bonding involving lignin functional groups on the pure face-centered cubic structure of metallic silver. Moreover, the lignin-modified silver nanoparticles generated a localized thermal effect upon near-infrared laser irradiation (808 nm), potentially allowing for targeted applications in the biomedical field. Our study showcases the potential of lignin as a renewable reducing and capping agent for silver nanoparticle synthesis, addressing some shortcomings of green synthesis approaches and contributing to the development of suitable nanomaterials. [ABSTRACT FROM AUTHOR]

Hydrotalcite–silver (HT−Ag) nanoparticles have been involved in various daily crucial applications, such as antibacterial, photocatalytic, adsorption, etc. There are many approaches to synthesizing silver nanoparticles (AgNPs) decorated on hydrotalcite (HT) surface and the most used approach is using a strong reducing agent. Thus, affordable but effective “green” reducing agents – Syzygium nervosum leaf extract, are taken into account in this work to solve several issues related to chemical reducing agents. This work aimed to assess the effect of Syzygium nervosum leaf extract as a reducing agent for green synthesis of AgNPs on HT through an optimizing process using response surface methodology (RSM) and the Box‐Benken model. The optimal conditions for the synthesis of AgNPs on HT include a reaction time of 6.15 hours, a reaction temperature of 50 °C, and the ratio of diluted Syzygium nervosum leaf extract to reduce AgNO3 of 50.37 mL/mg. Under the optimal conditions, the yield of the reduction reaction reached 77.54 %, close to the theoretical value of 76.97 %. The optimization model was suitable for the experiment data. Besides, the morphology, density, and characteristics of AgNPs on the surface of HT layers have been determined by using Ultraviolet‐visible spectroscopy, Field emission scanning electron microscopy (FESEM), High‐resolution transmission electron microscopy (HR‐TEM), selected area diffraction, X‐ray diffraction, Dynamic light scattering (DLS), Infrared (IR) spectroscopy, Fluorescence emission spectroscopy (FE), Brunauer‐Emmett‐Teller (BET) methods. The spherical AgNPs were synthesized successfully on the surface of HT with the average particle size of 13.0±1.1 nm. Interestingly, HT−Ag hybrid materials can inhibit strongly the growth of E. coli, S. aureus as well as two antibiotic resistance bacterial strains, P. stutzeri B27, and antibiotic resistance E. coli. Especially, the antibacterial activity quantification and durability of the HT−Ag hybrid materials were also tested. Overall, the HT−Ag hybrid materials are very promising for application in material science and biomedicine fields. [ABSTRACT FROM AUTHOR]

• Green synthesis of agnps from H. tiliaceus L. leaves extract. • Characterization of agnps was done by FE-SEM, HR-TEM, XRD and FT-IR. • Nanoparticles were stable in charge, crystalline in shape, with an average size of 88.10 nm in colloidal form. • AgNPs displayed potential antioxidant activity and broad range of antibacterial activity. • AgNPs have been shown to have potential as a catalyst in the dye degradation process. The present study reports the green synthesis of silver nanoparticles (AgNPs) from aqueous extract of Hibiscus tiliaceus L. leaves and their application in dye degradation, antioxidant, antimicrobial, and anticancer activities. Analysis using Fourier transform infrared (FT-IR) spectroscopy revealed that plant metabolite functional groups had a role in the reduction and stability of AgNPs, and X-ray crystallography (XRD) demonstrated that the AgNPs were in crystal form. The UV–vis spectroscopy, dynamic light scattering (DLS), and zeta potential investigations revealed that the AgNPs were formed with an average size of 88.10 nm in colloidal form and were stable (-49 mV). The field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HR-TEM) confirmed the shape and size of the AgNPs as spherical with a particle size of 30 - 35 nm, respectively. The AgNPs exhibited potential antioxidant activity by total antioxidant, DPPH, and reducing power assays. The biosynthesized AgNPs displayed a wide range of antibacterial activity on Gram-ve and Gram+ve bacteria by the zone of inhibition assay. AgNPs showed good anticancer activity on MCF-7 cells with an IC 50 value of 65.83 µg/mL. Furthermore, AgNPs acted as potential catalysts in combination with the reducing agent sodium borohydride (NaBH 4) for the degradation of methylene blue (MB), methylene orange (MO), and methylene green (MG) dyes. The degradation efficiency of catalyst AgNPs in the attendance of NaBH 4 for 15 min was noted to be 12.8 %, 26.92 %, and 47.56 % for MO, MB, and MG, respectively. The study concluded that green synthesized AgNPs could be highly applicable as an antioxidant, antimicrobial, and anticancer agents in the biomedical field. Furthermore, AgNPs could be helpful in the remediation of dye effluents. [ABSTRACT FROM AUTHOR]

Summary: The biological synthesis of functionalised nanoparticles using plant materials is considered more cost‐effective and eco‐friendly than chemical and physical methods. Hence, this study demonstrated the synthesis of silver nanoparticles (AgNPs) using Houttuynia cordata Thunb rhizome (HCR). The extract, prepared with distilled water, provided the required phytochemical components, such as flavonoids, terpenoids, phenols, proteins and other essential bio‐reducing agents. The AgNPs were exposed to reaction conditions, such as temperatures, pH values and AgNO3 concentrations, to identify the optimum synthesis condition and explore their impacts on particle size and antibacterial action against Gram‐positive (Staphylococcus aureus) and Gram‐negative (Salmonella enterica and Shigella dysenteriae) foodborne pathogens. The formation of AgNPs was indicated by specific surface plasmon resonance (SPR) intensity at 440–450 nm and colour changes under different reaction conditions. The structure and size distribution characterised by SEM and TEM revealed that the AgNPs exhibited a spherical‐shaped structure with irregular contours, regular distribution and aggregation. Although the average particle size ranged from 2 to 100 nm, relatively smaller sizes were detected at 10 mM (54.30), 80 °C (49.50 nm) and pH 6 (60.16 nm) conditions. The FTIR spectrum and EDX spectral signals affirmed the presence of reducing and stabilising agents in HCR extract, following the reduction of AgNPs from Ag+ to Ag0. AgNPs also exhibited excellent antibacterial activity against S. aureus, S. enterica and Sh. dysenteriae, indicating a broad spectrum for various applications in the food and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

Synthesis of silver nanoparticles with antibacterial properties using a one-pot green approach that harnesses the natural reducing and capping properties of cinnamon (Cinnamomum verum) bark extract is presented in this work. Silver nitrate was the sole chemical reagent employed in this process, acting as the precursor salt. Gas Chromatography-Mass Spectroscopy (GC-MS), High-Performance Liquid Chromatography (HPLC) analysis, and some phytochemical tests demonstrated that cinnamaldehyde is the main component in the cinnamon bark extract. The resulting bio-reduced silver nanoparticles underwent comprehensive characterization by Ultraviolet–Vis (UV-Vis) and Fourier Transform InfraRed spectrophotometry (FTIR), Dynamic Light Scattering (DLS), Transmission Electron Microscopy, and Scanning Electron Microscopy suggesting that cinnamaldehyde was chemically oxidated to produce silver nanoparticles. These cinnamon-extract-based silver nanoparticles (AgNPs-cinnamon) displayed diverse morphologies ranging from spherical to prismatic shapes, with sizes spanning between 2.94 and 65.1 nm. Subsequently, the antibacterial efficacy of these nanoparticles was investigated against Klebsiella, E. Coli, Pseudomonas, Staphylococcus aureus, and Acinetobacter strains. The results suggest the promising potential of silver nanoparticles obtained (AgNPs-cinnamon) as antimicrobial agents, offering a new avenue in the fight against bacterial infections. [ABSTRACT FROM AUTHOR]

This study defined a facile, inexpensive, and benign method to fabricate silver nanoparticles (AgNPs) using Tradescantia pallidum var. purpurea aqueous leaves extract for the first time. A UV–vis spectrum showed that the evaluated extract was promising for green synthesis of the SNP, with the highest peak of absorption between 450 and 550 nm. The phytochemicals present in the plant extract acts as capping and stabilization agent were confirmed by FTIR analysis. EDX proved the presence of Ag while SEM confirmed the AgNPs with the spherical shape. TEM revealed the average particle size was 23 nm. Additionally, AgNPs exhibited significant antibacterial activity and turbidimetry analysis showed MIC and MBC was in range of 25–100 µg/mL against both Gram positive and Gram-negative bacteria in Luria Bertani broth cultures. The AgNPs exhibited potent antibacterial activity against gram-positive bacteria, with a ZOI of 12 ± 0.25 mm produced against BacillusB subtilis showed the largest zones of inhibition, which were greater than the positive control (cefotaxime). The MIC values were 50 µg/mL for Pseudomonas aeruginosa, 25 µg/mL for Staphylococcus aureus and Escherichia coli, and 100 µg/mL for B. subtilis, confirming their significant antibacterial action. AgNPs inhibited the DDPH free radical scavenging activity with an IC50 = 91.87 µg/mL. It is believed that these AgNPs play a crucial role in determining the therapeutic potential of T. pallida due to their biological efficacy. [ABSTRACT FROM AUTHOR]

Nanobiotechnology has achieved great advances in the field of scientific research in past years. Nanoparticles (NPs) with diameters ranging from 1 to 100 nm are the focus of nanotechnology. Green synthesis of encapsulated silver nanoparticles (eAgNPs) was carried out by using Solidago virgaurea L. (SvL) and Tussilago farfara L. (TfL) extract as reducing/protecting agent. An UV–vis spectrophotometer was used to determine the optimal conditions to produce eAgNPs from plant extracts, including pH, temperature, plant extract, and mixing times. The optimal conditions for AgNPs synthesis from the SvL plant and TfL plant were (1:1) extract-AgNO3, 24 h mixing time, and a temperature of 25 °C. SEM, TEM, FTIR, and UV–Vis Spectrophotometer analyses were carried out to ascertain the surface characterization and structural properties of eAgNPs and the nanofibrous mats' physicochemical characteristics. The results of the analysis revealed that the eAgNPs are nanosized and contain silver. eAgNPs-doped nanofiber production from polylactide acid (PLA) polymer was realized by electrospin method. The required production parameters for the electrospinning method of producing PLA-based nanofibers were investigated. Nanofiber was produced by combining 0.5 and 1% AgNPs in an 8% PLA solution. The biological effects of the developed PLA@eAgNPs nanofibrous mats were examined in vitro. The antibacterial activity of nanofibers against E. coli was determined using the agar diffusion method. eAgNPs and PLA-based nanostructured materials are suggested as bioactive coatings capable of preventing the development of microbial population on surfaces relevant to healthcare. [ABSTRACT FROM AUTHOR]

The present work aims to study the possibilities of developing silver nanoparticles using natural extracts of grape pomace wastes originating from the native variety of Fetească Neagră 6 Șt. This study focused on investigating the influence of grape pomace extract obtained by two different extraction methods (classical temperature extraction and microwave-assisted extraction) in the phytosynthesis process of metal nanoparticles. The total phenolic content of the extracts was assessed using the spectrophotometric method with the Folin–Ciocâlteu reagent, while the identification and quantification of specific components were conducted through high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained nanoparticles were characterized by UV–Vis absorption spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM), along with assessing their antioxidant and antimicrobial properties against Gram-positive bacteria. The data collected from the experiments indicated that the nanoparticles were formed in a relatively short period of time (96 h) and, for the experimental variant involving the use of a 1:1 ratio (v/v, grape pomace extract: silver nitrate) for the nanoparticle phytosynthesis, the smallest crystallite sizes (from X-ray diffraction—4.58 nm and 5.14 nm) as well as spherical or semispherical nanoparticles with the lowest average diameters were obtained (19.99–23 nm, from TEM analysis). The phytosynthesis process was shown to enhance the antioxidant properties (determined using the DPPH assay) and the antimicrobial potential (tested against Gram-positive strains) of the nanoparticles, as evidenced by comparing their properties with those of the parent extracts; at the same time, the nanoparticles exhibited a selectivity in action, being active against the Staphylococcus aureus strain while presenting no antimicrobial potential against the Enterococcus faecalis strain. [ABSTRACT FROM AUTHOR]

Nanotechnology is developing into a fast-expanding discipline with applications in science and technology, and nanostructures are a crucial research tool in many fields. Due to their remarkable electrical, optical, magnetic, catalytic, and pharmacological capabilities, metal and metal oxide nanoparticles (NPs) have drawn study interest. Natural elements (plants, microorganisms, fungi, etc.) are utilized in a chemical-free, environmentally benign way to synthesize metals and metal oxides. The optical, electrical, and antimicrobial qualities of silver nanoparticle (AgNP) make them a popular choice. More than 200 active ingredients, including water-soluble, organic-soluble, and volatile chemicals, are found in Ganoderma. The main components are polysaccharides, adenosine, and terpenoids, each of which has exceptional therapeutic properties. This article explains the synthesis of Ag NPs by Ganoderma lucidum and tests the antibacterial effectiveness for use in biological applications. [ABSTRACT FROM AUTHOR]

Aim: The current work intends to examine the antibacterial activity of silver nanoparticles (AgNPs) mediated by Ocimum gratissimum stem extract against wound infections. Materials and Methods: To evaluate the membrane damage brought on by AgNPs, analyses of cytoplasmic leakage and protein leakage assays were performed. Results: The outcomes demonstrated that all of the tested bacterial strains were significantly resistant to the AgNPs' antibacterial activity. AgNPs damaged membranes and caused cellular contents to leak in the target pathogens, according to an examination of protein and cytoplasmic leakage. Conclusion: According to the current investigation, AgNPs mediated by Ocimum gratissimum stem extract may be effective antibacterial agents against microorganisms that cause wounds. [ABSTRACT FROM AUTHOR]

Recently, the eco-friendly production of silver nanoparticles (AgNPs) has become a focal point for researchers. In India, the quest for cost-effective, non-hazardous compounds suitable for reducing and stabilizing AgNPs has been limited. This study explores the synthesis of AgNPs using Elettaria cardamomom seed extract. We evaluated the efficiency of silver ion (Ag+) reduction and AgNP formation from an aqueous AgNO3 solution combined with the cardamom seed extract. Characterization of the AgNPs was carried out through UV-vis spectroscopy, XRD, SEM, EDAX, and FTIR. This research presents a practical and efficient strategy for AgNP production. A formulation using readily available 1 mm aqueous AgNO3, known for its accessibility and therapeutic properties, was created with cardamom seed extract serving as both a capping and reducing agent. Within 30 minutes, the silver ions were reduced to AgNPs, resulting in a change from golden to dark brown-reddish tint. Various analytical techniques, including UV-vis spectroscopy and Fourier-transform infrared spectroscopy, were employed for characterization. This study introduces an affordable, robust, and renewable method using E. cardamomum seeds for AgNP synthesis, which is entirely eco-friendly and devoid of toxic chemicals. AgNPs hold promise for applications in cancer treatment and diagnosis, setting them apart from other noble metals. [ABSTRACT FROM AUTHOR]

Synthetic dyes are persistent organic environmental pollutants that can cause extensive damage to living beings and to the ecosystem as a whole. Cost-effective, sustainable, and efficient strategies to deal with this type of pollution are necessary as it commonly resists conventional water treatment methods. Silver nanoparticles (AgNPs) synthesized using the aqueous extract from the leaves, stem, and fruits of Leucaena leucocephala (Leucena) were produced and characterized through UV–vis, TEM, EDS, SDL, XPS, XRD, and zeta potential, and they proved to be able to promote adsorption to remediate methylene blue and tartrazine pollution in water. The nanoremediation was performed and did not require direct exposure to sunlight or any special lamp or a specific reduction agent. The AgNPs produced using the extract from the leaves exhibited the best performance in nanoremediation and also presented antioxidant activity that surpassed the one from butylated hydroxytoluene (BHT). Consequently, it is an interesting nanotool to use in dye nanoremediation and/or as an antioxidant nanostructure. [ABSTRACT FROM AUTHOR]

The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed E. uniflora. Sterilized seeds of E. uniflora were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H2O2 and O2−. The ML-AgNO3 showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress. [ABSTRACT FROM AUTHOR]

Surface contamination by pathogenic and deteriorating microorganisms is a constant concern in the food industry. Therefore, in this study we evaluated the antimicrobial activity of silver nanoparticles (AgNPs), produced using the essential oil of Lippia origanoides Kunth and ethanolic extract of Hymenaea martiana Hayne, against bacteria used in milk processing. For this, AgNPs were synthesized and their antimicrobial activity was evaluated for minimum inhibitory and bactericidal concentrations, time-kill, interference on the biofilm, and application on the surfaces of different materials. AgNPs showed bacteriostatic and bactericidal activity against Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 33591), Escherichia coli (ATCC 25922), Salmonella enterica subsp. enterica serovar Choleraesuis (ATCC 10708), Listeria monocytogenes, Escherichia coli, Salmonella spp.,andPseudomonas aeruginosa,and interfered with biofilm formation more than with consolidated biofilms. A 1hour period was sufficient to reduce the bacterial cells, whereas a Minimum Duration for Killing (MDK) of 99% was reached after approximately 30 min. AgNPs were effective against bacteria attached to stainless steel and polyethylene, but ineffective on tile surfaces. Thus, owing to the growing microbial resistance and the need to develop new products based on the concepts of green chemistry, these AgNPs are presented as a new possibility for cleaning processes in the food industry [ABSTRACT FROM AUTHOR]

The green synthesized silver nanoparticles (AgNPs) with the aqueous extract of the leaves of household vegetable, Trigonella foenumgraecum were characterized and tested for their anti-oxidant and anti-bacterial efficacy with sixteen human clinical isolates. The Formation of AgNPs was confirmed with the surface plasmon spectra of the dark brown solution centered at 448 nm. The Particle size calculated with XRD data was 41.23 nm and the SEM size ranged between 52.56 nm and 80.51 nm. The presence of -NH and -OH groups that effectively react and reduce Ag+ ions to Ag0 was evident from the FT-IR spectra. The particles are predominantly spherical in shape and varied from 5 to 80 nm in size. As Suggested from the analysis by SEM and TEM, majority of them fall between 5 nm and 20 nm. A composite solution of AgNPs with polyvinyl alcohol (PVA) was electrospun into films and tested on 8 human clinical isolates including methicillin-resistant Staphylococcus aureus (MRSA). The PVA-AgNP films showed enormous activity against the MRSA prompting further study as wound dressings in the Wistar albino rat model. Although the animal study was abruptly terminated owing to the pandemic-induced lock down, the preliminary study results for 9 days suggest the potential clinical applications of these wound dressings. The synthesized AgNPs with and without PVA demonstrated good anti-oxidant activity and highly significant (P = 0.001) zones of inhibition with eighteen clinical isolates were tested. [ABSTRACT FROM AUTHOR]

Silver nanoparticles have shown inhibitory capabilities and its use in battling viral infections are strengthened by its potent antibacterial activity. Notably, since it directly affects antiviral efficacy, the proper choice of synthesis process for making silver nanoparticles is a vital element to be taken into consideration. The synthesis of silver nanoparticles for the treatment of viral and bacterial infections involves creating silver particles at the nanoscale with unique antimicrobial properties. Various methods exist for synthesizing silver nanoparticles, including chemical reduction, green synthesis, and physical methods. Chemical reduction involves using reducing agents to convert silver ions into silver nanoparticles. Green synthesis employs reducing agents in an environmentally friendly manner. Physical methods rely on techniques such as laser ablation or thermal evaporation to produce silver nanoparticles. In order to provide technological guidance for choosing antiviral silver nanoparticle manufacturing methods, this paper introduces and explores alternative synthesis methods for creating silver nanoparticles. [ABSTRACT FROM AUTHOR]

The circular economy, which attempts to decrease agricultural waste while also improving sustainable development through the production of sustainable products from waste and by-products, is currently one of the main objectives of environmental research. Taking this view, this study used a green approach to synthesize two forms of silver nanoparticles: coated silver nanoparticles with olive leaf extract (Ag-olive) and uncoated pure silver nanoparticles (Ag-pure), which were produced by the calcination of Ag-olive at 550 °C. The extract and the fabricated nanoparticles were characterized by a variety of physicochemical techniques, including high-performance liquid chromatography (HPLC), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Adult ticks (Hyalomma dromedarii) (Acari: Ixodidae) were used in this study to evaluate the antiparasitic activity of synthesized nanoparticles and extract. Furthermore, the antifungal activity was evaluated against Aspergillus aculeatus strain N (MW958085), Fuserium oxysporum (MT550034), and Alternaria tenuissiuma (MT550036). In both antiparasitic and antifungal tests, the as-synthesized Ag-olive showed higher inhibition activity than Ag-pure and olive leaf extract. The findings of this research suggest that Ag-olive may be a powerful and eco-friendly antiparasitic and antifungal agent. Ag-pure was also evaluated as a photocatalyst under sunlight for the detoxification of Eri-chrome-black T (EBT), methylene blue (MB), methyl orange (MO), and rhodamine B (RhB). [ABSTRACT FROM AUTHOR]

The synthesis of Cyanobacteria‐mediated nanoparticles has attracted great attention due to its ecofriendly, cost‐effective and biocompatible properties. Silver nanoparticles (AgNPs) are used in different areas such as medicine, household products, disinfectants or food storage. The present study aimed the biosynthesis of AgNPs from Cyanobacterial Chroococcus sp. and their antimicrobial activities on some important pathogen bacterial species. According to the obtained data, optimal conditions for synthesis of AgNPs were extract rate 3 : 1 v/v (cell extract/AgNO3), silver salt concentration 10 mM, pH 7, temperature 60 °C and time 30 minutes. The surface plasmon resonance peak of AgNPs was observed at 420–430 nm, and AgNPs were well‐dispersed spherical with an average size of 11–13 nm in diameter by TEM. SEM‐EDX analysis revealed a strong signal of silver. With FTIR analyzes, shift in the absorption bands after the AgNPs formation was confirmed. X‐ray diffraction analysis exhibited that the AgNPs were cubic crystalline shape. Zeta potential of AgNPs showed ‐ 20 mV. The AgNPs strong showed antibacterial activity on an opportunistic bacterial pathogens of Micococcus luteus, Bacillus subtilis and Pseudomonas aeroginosa. The effective and rapid synthesis of Chroococcus‐mediated AgNPs and their strong antimicrobial effects can create an important potential for their use in biomedical and environmental applications. [ABSTRACT FROM AUTHOR]

Herein, the prospective applications of green fabricated silver nanoparticles (Ag-NPs) within the biomedical field were investigated. The leaf aqueous extract of Portulaca oleracea L., a safe, cheap, and green method, was used to fabricate Ag-NPs. The maximum plasmon resonance of synthesized NPs has appeared at 420 nm. The various biomolecules present in the plant extract to assemble spherical Ag-NPs with sizes of 5–40 nm were analyzed using Fourier transform infrared and transmission electron microscopy. The Ag was the major content of the formed Ag-NPs with an atomic percent of 54.95% and weight percent of 65.86%, as indicated by EDX. The crystallographic structure of synthesized NPs was confirmed by the diffraction of the X-ray. The dynamic light scattering exhibits the homogeneity and mono-dispersity nature with a polydispersity index of 0.37 in the colloidal fluid and a zeta potential value of –36 mV. The synthesized Ag-NPs exhibited promising antimicrobial efficacy toward various prokaryotic and eukaryotic pathogenic microorganisms with low MIC values of 12.5 µg mL−1 and 6.25 µg mL−1, respectively. Additionally, the P. oleracea-formed Ag-NPs showed optimistic antioxidant activity assessed by DPPH and H2O2 assay methods with the highest scavenging percentages of 88.5 ± 2.3% and 76.5 ± 1.7%, respectively, at a concentration of 200 µg mL−1. Finally, the biosynthesized Ag-NPs showed high antiviral properties toward the hepatitis A virus and Cox-B4 with inhibition percentages of 79.16 ± 0.5% and 73.59 ± 0.8%, respectively. Overall, additional research is essential to explore the Ag-NP-based aqueous extract of P. oleracea for human health. In the current investigation the use of synthesized Ag-NPs as antimicrobial, antioxidant, and antiviral agents to protect against pathogenic microbes, degenerative diseases caused by various oxidative stresses, and deadly viruses is recommended. [ABSTRACT FROM AUTHOR]

Silver nanoparticles (AgNPs) are one of the most widely used antimicrobial agents. However, due to the potential problems of environmental pollution and high energy consumption, a green and efficient synthesis strategy of AgNPs is urgently required. Ovalbumin (OVA) is the most abundant protein in egg whites, and its extraction process is simple and productive. This paper reported a new green synthesis method of AgNPs by using OVA as an assistant accompanying with a visible light irradiation. Together with the reduction of silver ions, the uniformly dispersed OVA‐AgNPs nanocomposite could be formed within 30 min under xenon light irradiation by simple mixing AgNO3 and OVA in aqueous solution. The detailed mechanism study showed that tyrosine residue and peptide bonds in OVA played a major role in the reduction and stability of silver ions. In addition, in vitro antibacterial experiments indicated that 10 mg/L of OVA‐AgNPs, the minimum inhibitory concentration, has a good antimicrobial effect on both Gram‐positive and Gram‐negative bacteria, fungi, and some drug‐resistant bacteria species within 4 h of treatment, mainly due to the disruption of the structure of bacterial cell and the balance of reactive oxygen species. This work provides a new way for the green and efficient synthesis of AgNPs and shows good prospects for the applications in the field of biomedical materials and functional nanomaterials. [ABSTRACT FROM AUTHOR]

The increasing incidence of cancer cases and multi-drug-resistant bacteria, which are major threats to humankind, forces the research world to innovate new molecules to deal with them. The main aim of the present work is to prepare silver nanoparticles using macroalgal polysaccharides and to study biological activities. The silver nanoparticles (NPs) were prepared using polysaccharides extracted from the marine macro alga Portieria hornemannii by stirring them with 1 mM silver nitrate after 24 h at 90 °C. The formed silver nanoparticles were characterized using UV-visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR) analysis, Transmission Electron Microscopy (TEM) analysis, selected-area electron diffraction (SAED), and Energy Dispersive X-ray (EDX) analysis. UV-visible spectrum analysis revealed a surface plasmon peak at 380 nm, showing the development of silver nanoparticles. The nanoparticle size varied between 40 and 50 nm and the functional group was analyzed using FT-IR spectrum. The broadband was observed at 3304 cm-1 (hydroxyl and amino group) and the narrow band was observed at 2907 cm-1 (C-H stretching vibration), 1657 cm-1 (stretching of carbonyl groups), and 1001 cm-1 (C-O stretching vibration). The crystalline nature of silver NPs was confirmed by SAED. EDX analysis reveals the purity and the chemical composition of silver NPs. Nanoparticles were highly effective against Proteus mirabilis (24 mm zone of inhibition) and Bacillus substilis (24 mm zone of inhibition). The anticancer activity of the silver nanoparticles tested against colorectal adenocarcinoma cell lines increased at increasing concentrations of nanoparticles. [ABSTRACT FROM AUTHOR]

Objectives: Silver nanoparticles (AgNPs) are gaining increasing attention in biomedical applications due to their unique properties. Green synthesis methods are environmentally friendly and have demonstrated potential for AgNP production. This study explores the green synthesis of AgNPs using the methanolic extract of Euphorbia milii, a plant known for its medicinal properties. The primary objectives of this research were to synthesize AgNPs using E. milii extract, characterize the nanoparticles (NPs) using various techniques, and evaluate their antibacterial and enzyme inhibitory activities. Methods: E. milii plant extract was utilized for the green synthesis of AgNPs. The characterization of the NPs was performed through ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy (EDX). Antibacterial activity was assessed against Staphylococcus aureus, while enzyme inhibitory assays were conducted against urease, α-glucosidase, carbonic anhydrase II, and xanthine oxidase. Results: The synthesized AgNPs exhibited significant antibacterial effects, with a remarkable 20-mm zone of inhibition against S. aureus, surpassing the efficacy of the plant extract alone. Furthermore, the AgNPs demonstrated remarkable enzyme inhibition, achieving impressive percentages of 77.98% against α-glucosidase and 88.54% against carbonic anhydrase II. Half-maximal inhibitory concentration values for enzyme inhibition were highly promising, including 78.09 ± 1.98 µM for α-glucosidase, 0.22 ± 0.10 µM for carbonic anhydrase II, and 7.11 ± 0.55 µM for xanthine oxidase. Conclusion: In this study, AgNPs were successfully synthesized using E. milii extract and characterized using various techniques. The AgNPs exhibited significant antibacterial and enzyme-inhibitory activities, showcasing their potential for biomedical applications. [ABSTRACT FROM AUTHOR]

This research work explores the green synthesis of silver nanoparticles using Quassia indica(QI-Ag NPs), a natural plant extract, as a stabilizing and reducing agent. The synthesized QI-Ag NPs were characterized using various analytical techniques, including UV-Visible spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscopy (HR-TEM) and Selected Area Electron Diffraction (SAED). The UV-Visible analysis revealed a characteristic peak at 430 nm, indicating the successful formation of AgNPs. XRD analysis unveiled the crystalline nature of the nanoparticles, with four distinctive peaks corresponding to the silver crystallographic planes. SEM and EDX provided insights into the morphology and chemical composition of the QI-AgNPs. Moreover, TEM and SAED elucidated the structural attributes and crystallinity of the nanoparticles. The Ag NPs exhibited a spherical structure and crystalline nature, as supported by both SAED and XRD findings. The zeta potential of QI-Ag NPs exhibited a value of −24.2 mV. The synthesized QI-Ag NPs were evaluated for their photocatalytic potential, demonstrating a remarkable 97% degradation of Crystal Violet dye. Furthermore, comprehensive studies encompassing antioxidant, antimicrobial and cytotoxicity assessments were conducted, showcasing the multifaceted applications of these nanoparticles. This research underscores the promising potential of Q. indica-mediated silver nanoparticles as environmentally benign and versatile nanomaterials. [ABSTRACT FROM AUTHOR]

Green silver nanoparticles (AgNPs) possess tremendous promise for diverse applications due to their versatile characteristics. Coriander and other plant extracts have become popular for greenly synthesizing AgNPs as an economical, biocompatible, cost-effective, and environmentally beneficial alternative to chemical processes. In this study, we synthesized AgNPs from coriander leaves and evaluated their antibacterial, anti-inflammatory, antioxidant, and wound-healing acceleration properties in comparison to chemically synthesized AgNPs. The zeta potentials of AgNPs extracted from green and chemical processes were −32.4 mV and −23.4 mV, respectively. TEM images showed a cuboidal shape of green and chemical AgNPs with a diameter of approximately 100 nm. The FTIR spectra of green AgNPs showed an extreme absorption peak at 3401 cm−1, which signifies O-H stretching vibrations, typically linked to hydroxyl groups. In vitro results elaborated that AgNPs from coriander exerted a stronger effect on anti-Klebsiella pneumoniae (KP) through interrupting cell integrity, generating ROS, depleting ATP, and exhibiting significant antioxidant activity, compared with AgNPs synthesized chemically. In vivo experiments showed that AgNPs from coriander, as opposed to chemically manufactured AgNPs, greatly accelerated the healing of wounds contaminated with Klebsiella pneumoniae bacteria by effectively eliminating the bacteria on the wounds and stimulating skin regeneration and the deposition of dense collagen. In vivo assays further demonstrated that green AgNPs effectively enhanced Klebsiella pneumoniae-infected wound healing by extenuating local inflammatory responses and up-regulating VEGF and CD31 expression. In conclusion, green AgNPs significantly alleviated the inflammation without significantly harming the organism. [ABSTRACT FROM AUTHOR]

Natural products derived from medicinal plants offer convenience and therapeutic potential and have inspired the development of antimicrobial agents. Thus, it is worth exploring the combination of nanotechnology and natural products. In this study, silver nanoparticles (AgNPs) were synthesized from the leaf extract of Ginkgo biloba (Gb), having abundant flavonoid compounds. The reaction conditions and the colloidal stability were assessed using ultraviolet–visible spectroscopy. X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy (FTIR) were used to characterize the AgNPs. AgNPs exhibited a spherical morphology, uniform dispersion, and diameter ranging from ~8 to 9 nm. The FTIR data indicated that phytoconstituents, such as polyphenols, flavonoids, and terpenoids, could potentially serve as reducing and capping agents. The antibacterial activity of the synthesized AgNPs was assessed using broth dilution and agar well diffusion assays. The results demonstrate antibacterial effects against both Gram-positive and Gram-negative strains at low AgNP concentrations. The cytotoxicity of AgNPs was examined in vitro using the CCK-8 method, which showed that low concentrations of AgNPs are noncytotoxic to normal cells and promote cell growth. In conclusion, an environmentally friendly approach for synthesizing AgNPs from Gb leaves yielded antibacterial AgNPs with minimal toxicity, holding promise for future applications in the field of biomedicine. [ABSTRACT FROM AUTHOR]

Cotton tree (Bombax ceiba L.) flower stamen has been used widely as a northern Thai food ingredient. In this work, microwave extraction was applied to obtain the Bombax ceiba L. (BOMBAX) flower stamen extract for use as a reducing agent in the green synthesis of silver nanoparticles (AgNPs). The concentration of BOMBAX used was in the range of 0.05 to 0.25 wt%. Quasi-spherical and semi-rectangular shapes of AgNPs were obtained. The synthesized AgNPs showed a high mean zeta potential value of more than -30 mV, indicating the long-term stability and superior dispersity of AgNPs due to the repulsion of negative charges. Furthermore, the antibacterial potency of the synthesized AgNPs affords a good inhibition zone for both gram-negative and gram-positive bacteria, especially the gram-negative and antibiotic-resistant bacteria Pseudomonas aeruginosa, showing an inhibition zone diameter of 19.4±0.4 mm compared with that of chlophenicol (13.0±0.0 mm). This result suggests that the synthesized AgNPs present good colloidal stability and show good potential as an antibacterial agent for antibiotic-resistant bacteria, especially P. aeruginosa. [ABSTRACT FROM AUTHOR]

Antimicrobial natural polymer film with silver nanoparticles (AgNPs) biosynthesized using aqueous plant root extracts as reducing capping agents and for film formatting show extensive applicability for pathogenic microorganism problems. The formation of AgNPs was confirmed by transmission electron microscopy (TEM) and scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) techniques. The antimicrobial activity of biofilm with green AgNPs was analysed by inhibiting the growth of Gram-negative and Gram-positive bacteria culture using the Kirby–Bauer disk diffusion susceptibility test. Total phenolic content and antioxidant activity were slightly higher in aqueous extracts of Sym. Radix than in Sym. Radix/AgNPs. The antimicrobial effect of polymer film/AgNPs against selected test bacteria cultures was substantially more robust than with pure film. Pictures of AgNPs obtained by TEM revealed the presence of spherical-shaped nano-objects with an average size 27.45 nm. SEM–EDS studies confirmed the uniform distribution of metal nanoparticles throughout the biopolymeric matrix. Morphological studies of the surface showed that the obtained surface of the films was even, without holes or other relief irregularities. These apparent Symphyti radix polymer film/AgNPs' biological functions could provide a platform for fighting pathogenic bacteria in the era of multi-drug resistance. [ABSTRACT FROM AUTHOR]

Aim: In the study, the phenolic compounds of Salvia aethiopis/sclarea will be determined and the antioxidant and anti-inflammatory activities of the extracts of these plants and the synthesized silver nanoparticles (AgNP) will be examined in vitro. Materials and Methods: Extracts obtained from Salvia spp. (SE) collected from the Kyrenia region of Cyprus were used in the study. Methanol extracts were prepared and total phenolic, flavonoid and alkaloid contents were determined. The amount of phenolic compounds was then quantified and identified using HPLC. The antioxidant properties of Salvia aethiopis/sclarea were analyzed using various markers, including lipid peroxidation inhibition activity, 1,1-Diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) scavenging capacity. Additionally, their anti-inflammatory effects were investigated by proteinase inhibitor activity, heat-induced hemolysis and albumin denaturation analyses. Results: S. aethiopis and S. sclarea were contained high levels of the phenolic compounds isoquercetin and caffeic acid or naringenin and caffeic acid, respectively. After green synthesis of silver nanoparticles, the antioxidant and anti-inflammatory properties of plant extracts increased significantly (p<0.05). Conclusion: Our study found that silver nanoparticles synthesized using S. aethiopis/sclarea extract increased the antioxidant and anti-inflammatory effects of plant extracts and suggested that AgNP/SE could be used as a potential therapeutic agent in medicine. [ABSTRACT FROM AUTHOR]

Living organisms, particularly humans, frequently encounter microorganisms such as bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used in biomedical devices because of their antibacterial and antiviral properties. The study evaluates the efficacy of red laser and silver nanoparticles from grape seed extract (AgNPs-GSE) in reducing Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, which cause infections. The sample comprised three groups: a control group without laser irradiation (T0), Escherichia coli samples (A1 and A2) irradiated with a 405-nm diode laser at different times and concentrations of silver nanoparticles, and Staphylococcus aureus samples (A3 and A4) illuminated with a 405-nm diode laser at different times and concentrations. Bacteria in groups A2 and A4 were treated with a photosensitizer (PS) made from grape seed extracts, incubated for 10 min, and then irradiated for 90, 120, 150, and 180 s. The samples were cultured on TSA media, set at 37 °C, counted using a Quebec colony counter, and analyzed using ANOVA and Tukey tests with a significance level of p < 0.05. The study illustrated that the combination of 10 µl of AgNPs-GSE, exposure to a red laser at 405 nm, and an energy density of 3.44 J/cm2 effectively photoinactivated both Escherichia coli and Staphylococcus aureus bacteria. For Escherichia coli bacteria irradiated for 180 s with concentrations of 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, bacterial viability decreased by 64.50%, 70.74%, and 79.53%, respectively. Similarly, Staphylococcus aureus bacteria, subjected to irradiation for 180 s with concentrations of 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, demonstrated reductions in bacterial viability by 70.23%, 73.47%, and 85.04%, respectively. The findings from the present study indicate that at an energy density of 3.44 J/cm2, it was possible to inactivate Escherichia coli by 79.53% and Staphylococcus aureus by 85.04%. [ABSTRACT FROM AUTHOR]

Recently there have been a variety of methods to synthesize silver nanoparticles, among which the biosynthesis method is more noticeable due to features like being eco-friendly, simple, and cost-efficient. The present study aims for the green synthesis of silver nanoparticles from the extract of the three plants A. wilhelmsi, M. chamomilla, and C. longa; moreover, it pledges to measure the antibacterial activity against some variants causing a skin rash. The morphology and size of the synthesized silver nanoparticles were evaluated by UV.vis, XRD, SEM, and FTIR analyses. Then results showed a color alteration from light yellow to dark brown and the formation of silver nanoparticles. The absorption peak with the wavelength of approximately 450 nm resulting from the Spectrophotometry analysis confirmed the synthesis of silver nanoparticles. The presence of strong and wide peaks in FTIR indicated the presence of OH groups. The SEM results showed that most synthesized nanoparticles had a spherical angular structure and their size was about 10 to 20 nm. The highest inhibition power was demonstrated by silver nanoparticles synthesized from the extract combined from all three species against Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis (23 mm) which had a performance far more powerful than the extract. Thus, it can be understood that the nanoparticles synthesized from these three species can act as potential environment-friendly alternatives to inhibit some variations causing skin disorders; an issue that calls for further clinical studies. [ABSTRACT FROM AUTHOR]

The green synthesis of silver nanoparticles (AgNPs) using the cell-free supernatant of a Haematococcus pluvialis culture (CFS) was implemented in the current study, under illumination conditions. The reduction of Ag+ to AgNPs by the CFS could be described by a pseudo-first-order kinetic equation at the temperature range tested. A high reaction rate during synthesis and stable AgNPs were obtained at 45 °C, while an alkaline pH (pH = 11.0) and a AgNO3 aqueous solution to CFS ratio of 90:10 (v/v) proved to be the most effective conditions in AgNPs synthesis. A metal precursor (AgNO3) at the concentration range tested (1–5 mM) was the limited reactant in the synthesis process. The synthesis of AgNPs was accomplished under static and agitated conditions. Continuous stirring enhanced the rate of reaction but induced aggregation at prolonged incubation times. Zeta potential and polydispersity index measurements indicated stable AgNPs and the majority of AgNPs formation occurred in the monodisperse phase. The X-ray diffraction (XRD) pattern revealed the face-centered cubic structure of the formed AgNPs, while TEM analysis revealed that the AgNPs were of a quasi-spherical shape with a size from 30 to 50 nm. The long-term stability of the AgNPs could be achieved in darkness and at 4 °C. In addition, the synthesized nanoparticles showed antibacterial activity against Escherichia coli. [ABSTRACT FROM AUTHOR]

This study explores an eco-friendly approach to synthesizing silver nanoparticles (AgNPs) using soybean leaf extracts, employing a reaction with silver nitrate at 65 °C for 2.5 h. Optimal results were achieved at extract concentrations of 3.12 and 6.25 mg of the leaf mL−1, termed 3.12AgNP and 6.25AgNP, respectively. UV-Vis spectrophotometric analysis between 350 and 550 nm exhibited a peak at 410–430 nm, along with a color transition in the suspensions from pale yellow to brown, indicating successful synthesis. Dynamic light scattering (DLS) further delineated the favorable properties of these AgNPs, including nanometric dimensions (73–104 nm), negative charge, and moderate polydispersity, portraying stable and reproducible synthesis reactions. The bioreduction mechanism, possibly expedited by leaf extract constituents such as amino acids, phenolic acids, and polysaccharides, remains to be fully elucidated. Notably, this study underscored the potent nematicidal effectiveness of biosynthesized AgNPs, especially 6.25AgNP, against Pratylenchus brachyurus, which is a common plant-parasitic nematode in tropical soybean cultivation regions. In vitro tests illustrated significant nematicidal activity at concentrations above 25 µmol L−1, while in vivo experiments displayed a pronounced nematode population diminishment in plant roots, particularly with a 6.25AgNP rhizosphere application at concentrations of 500 µmol L−1 or twice at 250 µmol L−1, attaining a reproduction factor below 1 without any morphological nematode alterations. This research highlights the potential of 6.25AgNPs derived from soybean leaf extracts in forging sustainable nematicidal solutions, marking a significant stride toward eco-friendly phytonematode management in soybean cultivation. This novel methodology signals a promising avenue in harnessing botanical resources for nematode control and propelling a greener agricultural horizon. [ABSTRACT FROM AUTHOR]

In this study, designed as a dye-sensitive solar cell material which, bis-(2-(4-methylthiazol-5-yl) ethoxy) phthalocyaninato silicon (IV) compound (3) was obtained from the chemical reaction of SiPcCl2 and 4-methyl-5-thiazoleethanol. Silver nanoparticles (AgNPs) were synthesized using environmentally friendly and cost-effective green methods using leaf and flower extracts of Corydalis cava(CoV) and Nonea pulla(NP) plants. For the characterization of phthalocyanine compound and silver nanoparticles, Transmission electron microscopy (TEM), ¹H NMR, 13C NMR, X-Ray Diffraction (XRD), UV-visible, FT-IR and Mass spectrum devices were used. The power conversion efficiency of without doping compound 3 is 1.20. After doping this compound with CoVAgNPs and NPAgNPs, the power conversion efficiencies increased to 1.74 and 2.10. From this it is clearly seen that silver nanoparticles are effective. It is concluded that better photovoltaic materials can be created by using phthalocyanine and silver nanoparticles together. [ABSTRACT FROM AUTHOR]

Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium Azospirillum brasilense. The conditions and parameters for synthesis of biogenic nanoparticles using A. brasilense vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry. [ABSTRACT FROM AUTHOR]

Advances in nanotechnology have contributed to many innovative approaches in the forensic sciences, including the development of new techniques and protocols for latent fingermark detection. Among other nanomaterials, metal-based nanoparticles have been explored as suitable developers for fingermarks present on surfaces that challenge traditionally established methods. The present study explored, for the first time in the forensic science literature, the application of greenly synthesized silver nanoparticles (AgNPs) for latent fingermark surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis. A leaf extract of a native plant from the Cerrado biome was used for green synthesis of the AgNPs, and their hydrodynamic diameter, polydispersity index (PdI), and Zeta potential values were evaluated. Latent fingermarks were produced by three distinct donors and treated with α-CHCA matrix or AgNP suspension and were further investigated using commercial matrix assisted laser desorption/ionization (MALDI)-TOF MS equipment in the m/z range of 100–1000. Characterization results of the AgNPs indicated an average hydrodynamic diameter of 25.94 ± 0.30 nm, a PdI of 0.659 ± 0.085, and a Zeta potential of −33.4 ± 2.6 mV. The silver ions detected showed a relative intensity at least 20× higher for greenly synthesized AgNPs than for AgNO3 suspension, which may be advantageous for the detection of molecular species, especially olefins, present in forensic traces. The AgNP-based SALDI MS approach for the analysis of latent fingermarks showed intense ions at m/z 106.9, 215.8, and 322.7, referring to silver cation species that have been reported as important internal calibrants. The detection of components from endogenous and exogenous sources in latent fingermarks was achieved using the present approach. Greenly synthesized AgNPs may offer a new cost-effective, eco-friendly, and easily scaled up method for application in the chemical analysis of fingermarks. [ABSTRACT FROM AUTHOR]

Background: Diabetes mellitus (DM) is a non-communicable, life-threatening syndrome that is present all over the world. The use of eco-friendly, cost-effective, and green-synthesised nanoparticles as a medicinal therapy in the treatment of DM is an attractive option. Objective: In the present study, silver nanoparticles (AI-AgNPs) were biosynthesized through the green synthesis method using Azadirachta indica seed extract to evaluate their anti-diabetic potentials. Methods: These nanoparticles were characterized by using UV-visible spectroscopy, Fourier transform infrared spectrophotometers (FTIR), scanning electron microscopy (SEM), DLS, and X-ray diffraction (XRD). The biosynthesized AI-AgNPs and crude extracts of Azadirachta indica seeds were evaluated for anti-diabetic potentials using glucose adsorption assays, glucose uptake by yeast cells assays, and alpha-amylase inhibitory assays. Results: Al-AgNPs showed the highest activity (75 ± 1.528%), while crude extract showed (63 ± 2.5%) glucose uptake by yeast at 80 µg/mL. In the glucose adsorption assay, the highest activity of Al-AgNPs was 10.65 ± 1.58%, while crude extract showed 8.32 ± 0.258% at 30 mM, whereas in the alpha-amylase assay, Al-AgNPs exhibited the maximum activity of 73.85 ± 1.114% and crude extract 65.85 ± 2.101% at 100 µg/mL. The assay results of AI-AgNPs and crude showed substantial dose-dependent activities. Further, anti-diabetic potentials were also investigated in streptozotocin-induced diabetic mice. Mice were administered with AI-AgNPs (10 to 40 mg/kg b.w) for 30 days. Conclusions: The results showed a considerable drop in blood sugar levels, including pancreatic and liver cell regeneration, demonstrating that AI-AgNPs have strong anti-diabetic potential. [ABSTRACT FROM AUTHOR]

In the present research, Livistona chinensis leaf extracts were utilized as reductants to bio-fabricate silver nanoparticles (LC-AgNPs) and this was followed by the evaluation of their antioxidant, antibacterial, and anticancer potential. Multiple parameters were optimized for the formation and fidelity of LC-AgNPs. The color shift of the reaction mixture from yellow to dark brown confirmed the LC-AgNPs formation. UV/VIS spectroscopy exhibited a surface plasmon resonance (SPR) band at 436 nm. The Fourier transform infrared (FTIR) spectroscopy spectrum depicted phytochemicals in the plant extract acting as bio-reducers for LC-AgNPs synthesis. The XRD pattern confirmed the presence of LC-AgNPs by showing peaks corresponding to 2θ angle at 8.24° (111), 38.16° (200), 44.20° (220), and 64.72° (311). Zetasizer analysis exhibited size distribution by intensity of LC-AgNPs with a mean value of 255.7 d. nm. Moreover, the zeta potential indicated that the AgNPs synthesized were stable. The irregular shape of LC-AgNPs with a mean average of 38.46 ± 0.26 nm was found by scanning electron microscopy. Furthermore, the antioxidant potential of LC-AgNPs was examined using a DPPH assay and was calculated to be higher in LC-AgNPs than in leaf extracts. The calculated IC50 values of the LC-AgNPs and plant extract are 85.01 ± 0.17 and 209.44 ± 0.24, respectively. The antibacterial activity of LC-AgNPs was investigated against Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis as well as Staphylococcus aureus, and maximum potential was observed after 24 h against P. aeruginosa. Moreover, LC-AgNPs exhibited maximum anticancer potential against TPC1 cell lines compared to the plant extract. The findings suggested that LC-AgNPs could be used as antioxidant, antibacterial, and anticancer agents for the cure of free-radical-oriented bacterial and oncogenic diseases. [ABSTRACT FROM AUTHOR]

Silver nanoparticles (AgNPs) were successfully synthesized using an extract derived from Arctium lappa leaves, serving as both a reducing agent and stabilizer. This method proved to be rapid, environmentally friendly, and feasible at room temperature. The synthesized AgNPs were subjected to UV-vis spectrophotometry analysis, revealing a distinct peak at 422 nm, confirming the presence of silver nanoparticles. The spherical morphology of these nanoparticles was determined through atomic force microscopy (AFM) and scanning electron microscopy (SEM), displaying an average diameter of 40 nm. Moreover, the AgNPs obtained from the A. lappa leaf extract showcased notable antifungal capabilities, effectively impeding the growth of Phytophthora infestans and Alternaria. This study successfully demonstrated the potential of A. lappa leaf extract as an efficient and eco-friendly medium for the synthesis of silver nanoparticles. These nanoparticles not only exhibited distinctive characteristics, as revealed by UV-vis spectrophotometry and microscopy techniques, but also displayed significant antifungal activity. [ABSTRACT FROM AUTHOR]

Green synthesis of silver nanoparticles (AgNPs) from aqueous silver nitrate has been achieved using an extract of Ferula communis leaf as a capping, reducing, and stabilizing agent. The formation and stability of the green synthesized silver nanoparticles in the colloidal solution were monitored by absorption measurements. Silver nanoparticles were characterized by different analyses such as X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), and FT-IR spectroscopy. The average particle size of silver nanoparticles was determined by high-resolution transmission electron microscopy (HRTEM) and scanning electron microscopy (SEM) analyses. In this experiment, pregnant female mice were divided into four groups (G); G1 was the control and received phosphate-buffered saline, G2 received orally aqueous extract of F. communis leaf, G3 received orally AgNPs chemically prepared by NaBH4, and G4 received orally AgNPs prepared by aqueous extract of F. communis leaf. The diameter of AgNPs was 20 nm. AgNPs exhibited good catalytic reduction ability toward methyl orange in the presence of sodium borohydride with a rate constant of 2.95 x 10−4 s−1. The results revealed the occurrence of resorbed embryos in G2, G3, and G4 with different percentages. The livers of mothers and embryos at E14.5 in G2, G3, and G4 showed different levels of histopathological alteration and increase in GFAP and CTGF expressions compared with the control group. The study concluded that the oral administration of small-sized AgNPs (20 nm) prepared by Ferula extract had less toxicity than those prepared by the chemical method. [ABSTRACT FROM AUTHOR]