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Background: GJB2 mutations are among the most important causes of deafness, and their prevalence varies greatly among different countries and ethnic groups. This study aimed to determine the pathogenic mutation spectrum of GJB2 in patients with nonsyndromic hearing loss (NSHL) in Western Guangdong and to explore the pathogenic characteristics of the c.109G>A locus. Methods: In total, 97 NSHL patients and 212 normal controls (NC) were included in this study. Genetic sequencing analyses were performed on GJB2. Results: In the NSHL group, the main pathogenic mutations in GJB2 were as follows: c.109G>A, c.235delC, and c.299_300delAT with allele frequencies of 9.28%, 4.12%, and 2.06%, respectively. c.109G>A was the most frequently detected pathogenic mutation in this region. In the NC group, the allele frequency of c.109G>A among 30–50 years old subjects was markedly lower than that among 0–30 years old subjects (5.31% vs. 11.11%, p < 0.05). Conclusion: We found the pathogenic mutation spectrum of GJB2 in this region and showed that c.109G>A was the most common GJB2 mutation with unique characteristics, such as clinical phenotypic heterogeneity and delayed onset. Therefore, the c.109G>A mutation should be considered as an essential marker for routine genetic assessment of deafness, which can also be beneficial for preventing deafness. [ABSTRACT FROM AUTHOR]

Petroleum extraction, transportation, and consumption in the marine environment contribute to a large portion of anthropogenic oil spills into the ocean. While previous research focuses more on large oil spill accidents from oil tankers or offshore oil platforms, there are few systematic records on occasional regional oil spills. In this study, optical imagery from Landsat-8 OLI was used to detect oil slicks on the ocean surface through spatial analysis and spectral diagnosis in the northern South China Sea (NSCS). The source of the slicks was identified through datasets from traffic density and platform locations. A total of 632 oil slicks were detected in the NSCS from 2015 to 2019, where 57 were from platforms sources, and 490 were from ships. The average area of the detected slicks was 4.8 km2, and half of the slicks had areas <1.7 km2. Major oil spill hot spots included coastal Guangdong (ship origins), southeast and northeast Dongsha Island (ship origins), middle of south Beibu Gulf (ship and platform origins), and southeast Pearl River Estuary (platform origins). Through this study, we demonstrate the capability of medium-resolution optical imagery in monitoring regional oil spills. Such results and methods may help in near real-time oil spill monitoring and further environmental assessments. [ABSTRACT FROM AUTHOR]