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Your search keyword '"Growth and Development"' showing total 2 results
2 results on '"Growth and Development"'

1. Transcriptome analysis of biological pathways associated with heterosis in Chinese cabbage.

Author

Kong, Xiaoping, Chen, Lin, Wei, Tingzhen, Zhou, Hongwei, Bai, Chengfang, Yan, Xiangping, Miao, Zenjian, Xie, Jianming, and Zhang, Lugang

Subjects
*CHINESE cabbage, *HETEROSIS, *PLANT hormones, *ABSCISIC acid, *GENE expression, *TRANSCRIPTION factors, *AUXIN
Abstract

Chinese cabbage is an important vegetable in Asia, and high-yielding hybrids are needed to cope with the growing demand. A comparative transcriptome profiling was conducted to reveal the differentially expressed genes (DEGs) associated with heterosis in two hybrids relative to their parents. Our data suggests that heterosis is underlined by a significant upregulation of gene expression. High expression of DEGs in glycolysis and photosynthesis pathways in hybrids depicted their relation with growth and hybrid vigor. Besides, DEGs related to auxin, abscisic acid, ethylene and gibberellin were identified, implying that these hormones may boost the mechanisms of growth and developmental processes in the hybrids. Furthermore, transcription factors, including bHLH, ERF, MYB and WRKY were predicted to regulate downstream genes linked to hybrid vigor. Collectively, the present study will be helpful for a better understanding of the regulation mechanisms of heterosis to aid cabbage yield improvement. • Transcriptional mechanisms of heterosis in Chinese cabbage was studied. • Heterosis is underlined by a significant upregulation of gene expression. • Heterosis is mainly associated with non-additive expression. • Glycolysis and photosynthesis pathways were altered in hybrids. • Phytohormones related genes play crucial role in heterosis. [ABSTRACT FROM AUTHOR]

Published
2020
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2. ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties

Author

Sean R. Cutler, Yuming Hu, Dieter Buyst, Ann Cuypers, Kris Morreel, José C. Martins, Dominique Van Der Straeten, Petr Klíma, Dajo Smet, Klára Hoyerová, Thomas Depaepe, Jan Petrášek, Wout Boerjan, and Filip Vandenbussche

Subjects
0106 biological sciences, 0301 basic medicine, Auxin efflux, Physiology, Arabidopsis, Amino Acids, Cyclic, Gene Expression, Plant Science, ETHYLENE BIOSYNTHESIS, triple response, Quinolones, 01 natural sciences, chemistry.chemical_compound, herbicide, ABIOTIC STRESS, Arabidopsis thaliana, Homeostasis, heterocyclic compounds, Abscisic acid, SEEDLINGS, chemistry.chemical_classification, reactive oxygen species, APICAL HOOK DEVELOPMENT, response, food and beverages, ROOT HAIR DEVELOPMENT, ABSCISIC-ACID, Research Papers, Cell biology, quinoline carboxamide, chemical genetics, Biochemistry, auxin homeostasis, ethylene signaling, Growth and Development, CELL ELONGATION, GENE FAMILY, Root hair, Biology, 03 medical and health sciences, Auxin, Reactive oxygen species, SHOOT GRAVITROPISM, Auxin homeostasis, Indoleacetic Acids, Arabidopsis Proteins, Herbicides, triple, fungi, Biology and Life Sciences, Meristem, Ethylenes, biology.organism_classification, 030104 developmental biology, chemistry, PLANT-GROWTH, Seedlings, ARABIDOPSIS-THALIANA, 010606 plant biology & botany
Abstract

ACCERBATIN is an ethylene-mimicking small molecule that affects auxin homeostasis and ROS accumulation in etiolated seedlings. In light-grown plants, it exhibits auxin-like herbicidal properties., The volatile two-carbon hormone ethylene acts in concert with an array of signals to affect etiolated seedling development. From a chemical screen, we isolated a quinoline carboxamide designated ACCERBATIN (AEX) that exacerbates the 1-aminocyclopropane-1-carboxylic acid-induced triple response, typical for ethylene-treated seedlings in darkness. Phenotypic analyses revealed distinct AEX effects including inhibition of root hair development and shortening of the root meristem. Mutant analysis and reporter studies further suggested that AEX most probably acts in parallel to ethylene signaling. We demonstrated that AEX functions at the intersection of auxin metabolism and reactive oxygen species (ROS) homeostasis. AEX inhibited auxin efflux in BY-2 cells and promoted indole-3-acetic acid (IAA) oxidation in the shoot apical meristem and cotyledons of etiolated seedlings. Gene expression studies and superoxide/hydrogen peroxide staining further revealed that the disrupted auxin homeostasis was accompanied by oxidative stress. Interestingly, in light conditions, AEX exhibited properties reminiscent of the quinoline carboxylate-type auxin-like herbicides. We propose that AEX interferes with auxin transport from its major biosynthesis sites, either as a direct consequence of poor basipetal transport from the shoot meristematic region, or indirectly, through excessive IAA oxidation and ROS accumulation. Further investigation of AEX can provide new insights into the mechanisms connecting auxin and ROS homeostasis in plant development and provide useful tools to study auxin-type herbicides.

Published
2017

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