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APEX (Airborne Prism EXperiment) is a high spectral and spatial resolution hyperspectral sensor developed by a Swiss-Belgian consortium on behalf of the European Space Agency. Since the acceptance of the instrument in 2010, it has been operated jointly by the Flemish Institute for Technological Research (VITO, Mol, Belgium) and the Remote Sensing Laboratories (RSL, Zurich, Switzerland). During this period, several flight campaigns have been performed across Europe, gathering over 4 Terabytes of raw data. Following radiometric, geometric and atmospheric processing, this data has been provided to a multitude of Belgian and European researchers, institutes and agencies, including the European Space Agency (ESA), the European Facility for Airborne Research (EUFAR) and the Belgian Science Policy Office (BelSPO). The applications of APEX data span a wide range of research topics, e.g. landcover mapping (mountainous, coastal, countryside and urban regions), the assessment of important structural and (bio)physical characteristics of vegetative and non-vegetative species, the tracing of atmospheric gases, and water content analysis (chlorophyll, suspended matter). Recurrent instrument calibration, accurate flight planning and preparation, and experienced pilots and instrument operators are crucial to successful data acquisition campaigns. In this paper, we highlight in detail these practical aspects of a typical APEX data acquisition campaign. [ABSTRACT FROM AUTHOR]