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Abstract Background In patients undergoing total joint arthroplasty (TJA), the administration of dexamethasone may contribute to perioperative blood glucose (BG) disturbances, potentially resulting in complications, even in patients without diabetes. This study aimed to demonstrate the impact of different administration regimens of dexamethasone in postoperative BG levels. Methods In this randomized, controlled, double-blind trial, 136 patients without diabetes scheduled for TJA were randomly assigned to three groups: two perioperative saline injections (Group A, placebo); a single preoperative injection of 20 mg dexamethasone and a postoperative saline injection (Group B), and two perioperative injections of 10 mg dexamethasone (Group C). Primary outcomes were the postoperative fasting blood glucose (FBG) levels. Secondary outcome parameters were the postoperative postprandial blood glucose (PBG) levels. Postoperative complications within 90 days were also recorded. Risk factors for FBG ≥ 140 mg/dl and PBG ≥ 180 mg/dl were investigated. Results Compared to Group A, there were transient increases in FBG and PBG on postoperative days (PODs) 0 and 1 in Groups B and C. Statistical differences in FBG and PBG among the three groups were nearly absent from POD 1 onward. Both dexamethasone regimens did not increase the risk for postoperative FBG ≥ 140 mg/dl or PBG ≥ 180 mg/dl. Elevated preoperative HbA1c levels may increase the risk of postoperative FBG ≥ 140 mg/dl or PBG ≥ 180 mg/dl, respectively. Conclusion Perioperative intravenous high-dose dexamethasone to patients without diabetes has transient effects on increasing BG levels after TJA. However, no differences were found between the split-dose and single high-dose regimens. The elevated preoperative HbA1c, but not the dexamethasone regimens were the risk factor for FBG ≥ 140 mg/dl and PBG ≥ 180 mg/dl. Trial registration Chinese Clinical Trail Registry, ChiCTR2300069473. Registered 17 March 2023, https://www.chictr.org.cn/showproj.html?proj=186760 .

Background:. With the accumulation of experience and evidence, guidelines for invasive intervention for acute pancreatitis (AP) have continuously evolved, followed in Peking Union Medical College Hospital (PUMCH). We aimed to review AP case series to help understand the impact of guideline evolution on the management and prognosis of AP in real-world practice. Methods:. This was a single-center study of AP patients who had received invasive interventions from 1988 to 2022. Patient demographics, baseline severity, imaging findings, and the indication, timing, and specific modalities of invasive interventions were collected. The composite primary endpoint was death during hospitalization or major complications after intervention. Other endpoints included pancreatic fistula, incision infection, number of interventions, length of intensive care stay, length of hospitalization, and total medical cost. Results:. A total of 195 patients were included. The most common indication for invasive intervention was suspected infection, followed by persistent symptoms. The step-up and delayed strategies reduced the incidence of major complications or death. Over 35 years, the number of patients requiring surgery has gradually declined, and more patients need only minimally invasive procedures for remission. The incidence of the primary outcome decreased as well as the duration of hospitalization. Conclusions:. The management of AP patients at PUMCH in the last 35 years has undergone therapeutic guideline changes that support the efficacy and safety of the deferred step-up strategy in real-world practice.

This study introduces a brazing technique for joining Al0·3CoCrFeNi high-entropy alloy (HEA) utilizing a FeCoCrNiCu/Ti composite interlayer. The primary objectives of this investigation encompass an examination of the microstructure of the Al0·3CoCrFeNi joint, as well as an evaluation of the shear strength of the brazed joints under diverse brazing parameters. The characteristic microstructure of the Al0·3CoCrFeNi brazing seam is comprised of Ti (s, s), Cu (s, s) from the FeCoCrNiCu interlayer, and face-centered cubic (FCC) phases containing more than five elements. When the brazing temperature increases from 1100 °C to 1180 °C, there is a gradual augmentation in the diffusion of Ti element and subsequent reactions involving Cu, Ti, and Ni. Furthermore, the impact of holding time on the brazing seam is investigated. The findings suggest that prolonging the holding time has a relatively negligible effect on the reaction layer formed by the Ti foil. However, it does lead to a reduction of the Cu (s, s) content within the FeCoCrNiCu interlayer. Notably, the highest shear strength reaches up to 280 MPa when the joint brazed at 1160 °C for 10 min. In addition, the shear strength did not decrease below 800 °C. During the shear test, ductile fractures are observed in the joint, indicating favorable mechanical properties. Overall, this study successfully demonstrates the potential of the proposed brazing technique employing a FeCoCrNiCu/Ti composite interlayer for the effective joining of Al0·3CoCrFeNi HEA. By optimizing the brazing parameters, it is possible to achieve robust joints with exceptional mechanical characteristics.

Narrow gap oscillating laser welding with filler wire is a promising technique for joining high-strength steels in construction machinery. In order to investigate the influence of beam oscillation on the surface morphology and microstructure of weld joints of two types of commonly-used high-strength steels, a comparative study between oscillation welding and non-oscillation welding was conducted. High-speed photography technique was employed to reveal the microstructure and mechanical properties of the dissimilar joints. The results showed that laser beam oscillation produces two effects: one was to enlarge the heated surface of molten pool, while the other was to stir the liquid metal in molten pool periodically. Under the first effect, the fusion between sidewall and weld bead layers was promoted, while under the latter, coarse columnar grains ceased to appear, but finer grains were formed. The weld joints between Q355 and 35 steels exhibited excellent tensile impact bending performance, which was attributed to the grain refinement effect. The results also demonstrated that the oscillation welding with a contact angle less than 50° could avoid lack of fusion in multi-pass wire-filling laser welding. The influence of the oscillation welding process on the quality of welding was further discussed.

Abstract Background Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Phosphofructokinase 1 (PFK-1) is one of member of PFK, which plays an important role in reprogramming cancer metabolism, such as lactylation modification. Zinc finger E-box-binding homeobox 1 (ZEB1) has been demonstrated to be a oncogene in many cancers. Therefore, this study was performed to explore the effects of PFK-1 on the lactylation of ZEB1 in BC development. Methods Cell viability was measured using the CCK-8 kit. The glucose assay kit and lactate assay kit were used to detect glucose utilization and lactate production. The DNA was purified and quantified by qRT-PCR. Results In the present study, we found that ZEB1 expression levels were significantly elevated in bladder cancer cells. Impaired PFK-1 expression inhibits proliferation, migration, and invasion of BC cells and suppresses tumour growth in vivo. We subsequently found that knockdown of PFK-1 decreases glycolysis, including reduced glucose consumption, lactate production and total extracellular acidification rate (ECAR). Mechanistically, PFK-1 inhibits histone lactylation of bladder cancer cells, and thus inhibits the transcription activity of ZEB1. Conclusion Our results suggest that PFK-1 can inhibit the malignant phenotype of bladder cancer cells by mediating the lactylation of ZEB1. These findings suggested PFK-1 to be a new potential target for bladder cancer therapy.

Abstract Chemotherapeutic drugs will affect the process of spermatogenesis. However, most current studies on the effects of chemotherapeutic drugs on spermatogenesis are based on mouse models, with a shortage of human body evidence. In addition, the mechanism of chemotherapeutic drugs causing spermatogenesis disorder is not clear. Therefore, we have collected the testicular tissues of an inguinal-lipoma patient whose testes were resected after chemotherapy and a patient who had normal spermatogenesis disorder and underwent single-nucleus RNA sequencing (snRNA-Seq). After quality control, we obtained a total of 27,957 high-quality cells, including 18,612 normal cells and 9,345 drug-treated cells, which were all used in analyzing the mechanism of chemotherapeutic drugs causing spermatogenesis disorder. This study has provided data resources and references for exploring the mechanism of chemotherapeutic drugs causing spermatogenesis disorder with the insight of protecting the spermatogenic abilities of male tumor patients receiving chemotherapy.

Abstract Implant-associated infections due to the formation of bacterial biofilms pose a serious threat in medical healthcare, which needs effective therapeutic methods. Here, we propose a multifunctional nanoreactor by spatiotemporal ultrasound-driven tandem catalysis to amplify the efficacy of sonodynamic and chemodynamic therapy. By combining piezoelectric barium titanate with polydopamine and copper, the ultrasound-activated piezo-hot carriers transfer easily to copper by polydopamine. It boosts reactive oxygen species production by piezoelectrics, and facilitates the interconversion between Cu2+ and Cu+ to promote hydroxyl radical generation via Cu+ -catalyzed chemodynamic reactions. Finally, the elevated reactive oxygen species cause bacterial membrane structure loosening and DNA damage. Transcriptomics and metabolomics analysis reveal that intracellular copper overload restricts the tricarboxylic acid cycle, promoting bacterial cuproptosis-like death. Therefore, the polyetherketoneketone scaffold engineered with the designed nanoreactor shows excellent antibacterial performance with ultrasound stimulation and promotes angiogenesis and osteogenesis on-demand in vivo.

Objectives The objective of this study was to quantify the morphology, composition, and asymmetry of the paravertebral extensor muscles (PSEMs) in patients with cervical ossification of the posterior longitudinal ligament (OPLL) who had different modified K‐line (mK‐line) and the minimum interval between the mK‐line and OPLL (INTmin) values and to investigate the relationship between PSEMs and symptoms and outcomes following laminoplasty. These original findings elucidated that the atrophy of PSEMs could predict decompression outcomes and provided a theoretical basis for paraspinal muscle rehabilitation. Methods A total of 94 consecutive patients who underwent laminoplasty for OPLL between January 2020 and January 2022 were enrolled in this retrospective study. The relative cross‐sectional areas (rCSA), functional cross‐sectional areas (rFCSA), and FCSA/CSA ratio of the multifidus (MF), semispinalis cervicalis (SSCe), semispinalis capitis (SSCa), and splenius capitis (SpCa) were measured at the C3–C7 segments on cervical magnetic resonance imaging (MRI). This study compared the differences between the mK‐line (+) group and the mK‐line (−) group, as well as between the INTmin

In recent years, new energy vehicles (NEVs) have taken the world by storm. A large number of NEV batteries have been scrapped, and research on NEV battery recycling is important for promoting the sustainable development of NEVs. Battery recycling is an important aspect of the sustainable development of NEVs. In this study, we conducted an in-depth analysis of the current status of research on NEV battery recycling from a new perspective using bibliometric methods and visualization software. This study shows that research targeting the recycling of NEV batteries is growing rapidly, and collaborative networks exist among researchers from different countries, institutions, and fields. The focus of research has shifted from lead-acid batteries to lithium batteries, and the supply chain and circular economy related to NEV battery recycling is an emerging research hotspot. Based on our analysis, we propose that the government should establish policies to improve the recycling networks at the collection stage and provide subsidies to attract consumers. Enterprises should develop low-cobalt and cobalt-free technologies, utilize green solvents, and develop new battery swap modes. The establishment of an information platform is conducive to the further development of collaborative networks.

Reactive air brazing (RAB) has been widely applied in fabricating solid oxide fuel cell stacks, gas separators, sensors, and other fields due to the joint good oxidation resistance. Nevertheless, a methodical analysis of fundamental principles, development process, and application scope has yet to be reported since RAB was proposed in 2000. Although various braze systems have been developed, a comprehensive comparison and classification are still absent, making it difficult to guide the selection of RAB braze. Besides, this technique faces several intractable issues, such as excessive interfacial reactions, large thermal mismatch, etc., seriously restricting the RAB application. Hence this review elucidates the basic information, braze systems, existing issues, and corresponding solutions of RAB. The joining mechanism, development history, and application fields are explicitly introduced. Reported braze systems are analyzed in-depth and divided into three categories according to reactivity. Further, we outline four issues of RAB: excessive interfacial reaction, excessive thermal expansion coefficient of braze, limited aging performance, and high-temperature softening of braze, and discuss the corresponding solutions. Finally, our review proposed several potential improvements and possible future research trends in the RAB technique, aiming to guide the future development of RAB and offer new insights to broaden its application range.

BackgroundIschaemic heart failure with reduced ejection fraction (HFrEF) caused by coronary artery disease accounts for the largest proportion of heart failure cases with the worst prognosis. Coronary artery bypass grafting (CABG) is the most effective treatment for ischaemic HFrEF. On-pump and off-pump are the two surgical methods used for CABG. Whether patients with HFrEF should undergo on- or off-pump CABG is controversial in coronary heart disease surgery. The left ventricular end-systolic volume index (LVSEVI) is the gold standard for evaluating the severity of left ventricular remodelling; however, its effect on the perioperative risk and long-term survival rate of patients with HFrEF undergoing CABG remains unclear.MethodsThis single centre prospective cohort analysis included 118 coronary heart disease patients with symptoms and signs of heart failure and a left ventricular ejection fraction (LVEF) of

In the paper, the authors simply review recent results of inequalities, monotonicity, signs of determinants, determinantal formulas, closed-form expressions, and identities of the Bernoulli numbers and polynomials, establish an identity involving the differences between the Bernoulli polynomials and the Bernoulli numbers, present two identities among the differences between the Bernoulli polynomials and the Bernoulli numbers in terms of a determinant and a partial Bell polynomial, and derive a determinantal formula of the differences between the Bernoulli polynomials and the Bernoulli numbers.

Vacuum brazing of TiAl alloy is achieved using TiZrNiCu fillers and BNi-2 fillers. The oxidation behavior of the two kinds of TiAl alloy joints is investigated in air at 800 °C. The influence of high-temperature oxidation on the microstructure and mechanical properties of the joints are characterized by scanning electron microscopy, X-ray diffraction, transmission electron microscopy, and shear tests. It is found that long-term oxidation does not have a large effect on the microstructure of joints. However, the fillet of the brazed joint achieved via BNi-2 fillers forms longitudinal solidification cracks, which proved that the brazed joint using TiZrNiCu fillers shows more excellent oxidation resistance.

Glass/Glass-ceramic sealing is always accompanied by issues of high porosity and brittle joints. To migrate these problems, an Ag foam was innovatively induced as an interlayer in the process of glass sealing the yttria-stabilized zirconia (YSZ) ceramic to Mn–Co spinel-coated Crofer 22 H stainless steel. Interlayer-added joints showed a significant reduction in porosity, and the shear strength was improved to 52.3 MPa, with an enhancement of 37.8 %. The influence of interlayer on the microstructure of the joint has been investigated and the formation process of the joint is illustrated. The Ag interlayer expels gases from the sealant material through expansion at high temperatures and promotes finer nucleation grains of crystallites integrating with the interlayer, reducing the porosity and alleviating the brittleness as well.

Abstract Background Ovarian cancer (OC) causes the highest rates of mortality among women’s genital tract malignancies. Micro-ribonucleic acid (miRNA), the most abundant long noncoding RNAs transmitted by exosomes, has been revealed to be a potential marker for OC since 2008. In this study, we aimed to determine the possible roles of miRNAs derived from exosomes in the early diagnosis of OC through miRNA microarray, besides, exploring the underlying mechanisms of miRNAs in the OC progression. Methods We isolated exosomes from high invasive OC cell line HO8910PM and its parent cell line HO8910 using transmission electron microscopy and western blot, and performed miRNA microarray to identify the exosome-transmitted miRNA from the two cell lines, respectively. The expression profile was obtained by quantitative analysis, and then the differentially expressed individuals were screened. miRNA-30a-5p, a stable miRNA in both cells of our sequencing data was set for further study. MiR-30a-5p mimics, inhibitor and their corresponding negative controls were applied in OC cells. Then the cell proliferation, migration, and invasion of different groups were analyzed via cell counting-kit 8 (CCK8), wound healing, and Transwell analyses. Besides, ZBE2 and LDH2 expressions were detected by qRT-PCR. Results Combined with the data report of miRNA microarray technology, we set miR-30a-5p as our target miRNA to analyze its molecular function in regulating proliferation, migration, and invasion in OC cells. Our results showed that the miR-30a-5p overexpression could significantly enhance the capability of proliferation, migration, and invasion of HO8910 and HO8910PM cells, whereas the miR-30a-5p inhibition showed the opposite tendency (all P

Laminated composites have been extensively researched due to their excellent mechanical properties, but their application in superwetting fields has been rarely reported. In this work, the surfaces with extreme wettability were prepared based on laminated composites. The Ti6Al4V/304 stainless steel (304 SS) micro-laminated composites were fabricated by diffusion bonding the Ti6Al4V foils and 304 SS foils with the thickness of 10–50 μm in vacuum. The typical interfacial microstructure and the effect of diffusion temperature on the microstructure were investigated. The bonded interface of joints is mainly composed of β-Ti, Ti2Ni, FeTi, Fe2Ti, Fe–Cr from the Ti6Al4V side to the 304 SS. The effect of diffusion temperature on the microstructure was researched. Based on the prepared Ti6Al4V/304 SS micro-laminated composites, an array structure with micro grooves was fabricated through the simple chemical etching. The 304 SS foils were etched to form grooves, while Ti6Al4V foils still remained as bulges. Due to surface modification and rough morphology, the etched surfaces exhibited the excellent hydrophobicity. Increasing the width and depth of grooves and decreasing the width of bulges led to the higher water contact angles and improved hydrophobicity. The highest water contact angles of 148.3° has been achieved under the conditions of grooves with a width of 50 μm, a depth of 60 μm, and the bulges with a width of 10 μm. The surfaces exhibited nearly superhydrophobicity and performed self-cleaning property. The processing method of this work could expand the application of laminated composite in extreme wettability fields.

K418 alloy was prepared by selective laser melting (SLM) at different laser powers (160∼240 W) in this paper. The influence of laser power on the densification behavior, microstructure, microhardness, and tensile properties was studied systematically. The results showed that the densities of the material increased rapidly first and then decreased slowly with the increase of laser power. When the laser power increased from 160 W to 240 W, the surface defects of the specimen decreased, the microstructure changed from columnar dendrites to cellular, and the grains grew in the direction preferentially. When the laser power is 200 W, the grain size is the smallest, the content of small-angle grain boundaries is more, the Schmidt factor is less, and then the best mechanical properties are achieved, at which the microhardness reaches 362.89 ± 5.01 HV, the tensile strength reaches 1244.35 ± 70.1 MPa, the yield strength reaches 863.89 ± 23.1 MPa, and the elongation of the material reaches a maximum of 12.53 ± 0.71 %. In addition, the fracture mode of the material is a mixed tough-brittle fracture mechanism.

Abstract Based on the mining conditions of shallow close coal seams in the Jurassic coalfield of northern Shaanxi, in order to explore the evolution and development height prediction of water-conducting fracture zone (WCFZ), combining with measured statistical analysis, physical simulation, numerical calculation, and theoretical analysis, the evolution of mining-induced fractures and the development characteristics of WCFZ are analyzed, and the development height predicting method of WCFZ in shallow close coal seams mining is proposed. The results show that in shallow single coal seam mining, during the stage of incritical mining, the height of the WCFZ increases in a “jumping” pattern with the main roof periodic caving. After critical mining, it is basically stable. Its development height is positively correlated with the mining height. The ratio of development height to mining height is generally 20–30, and the change is not significant with the mining height, overall, the distribution of the WCFZ is “saddle shaped”. Based on close distance coal seams repeated mining, the primary fractures act as activation development when the interburden broken completely, the development of WCFZ at the mining boundary is significantly intensified, and ratio of the height to mining height in repeated mining is obviously lower than that in single seam mining, which is generally 18–25. The traditional empirical formula cannot be applied to the development prediction in shallow close coal seams mining, with an error of 10–50%. Considering the mining height, interburden thickness, and roof caving characteristics, the development height predicting method of WCFZ in shallow close coal seams mining is proposed. Compared with measured results, the calculation error is basically within 15%, which has good prediction accuracy, The research results can provide a theoretical basis for predicting the height of WCFZ in shallow close coal seams mining in the Jurassic coalfield of northern Shaanxi.

Abstract Patients with inflammatory bowel disease (IBD) always suffer from severe abdominal pain and appear to be at high risk for colorectal cancer. Recently, the co-delivery of targeted drugs and gut microbiota has developed into an attractive strategy. A new strategy using gut microbiota fermentation to overcome the interspace diffuse resistance from the mucus layer to control drug release in inflammatory bowel sites (IBS sites) has not yet been available. Here, we designed an alginate hydrogel microsphere encapsulating bifidobacterium (Bac) and drug-modified nanoscale dietary fibers (NDFs). The hydrogel microsphere is responsible for protecting drugs from acidic and multi-enzymatic environments and delivering drugs to the colorectum. Subsequently, the fermentation of Bac by digesting NDFs and proteins as carbon and nitrogen sources can promote drug release and play a probiotic role in the gut microbiota. In vitro evidence indicated that small-sized NDF (NDF-1) could significantly promote short-chain fatty acid (SCFA) expression. Notably, NDF-1 hydrogel microspheres showed a boost release of 5-ASA in the IBS sites, resulting in the amelioration of gut inflammation and remodeling of gut microbiota in chronic colitis mice. This study developed a controlled release system based on microbial fermentation for the treatment of IBD.

Joining C/C composites to Ni-based single crystal alloy DD3 can significantly broaden their industrial application. The currently used active brazing method (ABM) may cause harmful reactions with Ni-based alloys, generating brittle compounds and deteriorating joint performance. In this work, a two-step method involving chromium carbide (Cr–C) surface modification and brazing using pure Cu filler is first developed to explore avoiding brittle compounds in joints. Surprisingly, the brazed seam of joints is mainly composed of (Cu, Ni) solid solution (s, s) without generating brittle compounds. At the DD3 interface, only (Ni, Cu) (s, s) and (Cu, Ni) (s, s) layers are formed. Besides, (W, Mo) (s, s) particles are precipitated nearby the DD3 substrate. For the C/C interface, the Cu filler is tightly joined to the Cr–C layer without forming new phases. The joint microstructure has no significant change with the variation of the Cr–C layer thickness. As increased the Cr–C layer thickness, the joint strength first increases and then decreases. The maximum shear strength of ∼32 MPa is obtained when the Cr–C layer thickness is ∼4.6 μm. The joint shear strength decreases with increased testing temperatures, which can still be maintained at ∼28 MPa at 600 °C. The surface modification and brazing strategy in this work can be further applied to the joining of other carbon materials and ceramics with poor wettability.

In recent years, cadmium (Cd) contamination in agricultural soil has emerged as a significant global environmental issue, posing irreversible harm to crops and human health. As a result, efficient soil remediation techniques are urgently needed. For this issue, synergistic remediation by material and plant is an effective approach. In this study, a natural and green adsorption material (starch/montmorillonite composite, SMC) of Cd was prepared, which was further employed in synergistic remediation toward soil Cd contamination with the cadmium hyperaccumulator plant Bidens bipinnata. The results of the pot experiment demonstrated that an available Cd removal rate of 77.92 could be obtained, and the results of the field experiments demonstrate that the concentrations of Cd in contaminated soil could be reduced below the risk-screening values for agricultural land. Further analyses, including a microbial community diversity study, changes in soil BCR fraction components, and a TCLP toxicity leaching experiment, unequivocally elucidated that the synergy of SMC and Bidens bipinnata enhanced the remediation efficiency of Cd in contaminated soil. This study confirmed the application potential of the synergy of SMC and Bidens bipinnata toward Cd-contaminated soil.

Evapotranspiration (ET) accounts for over half of the moisture source of Asian monsoon rainfall, which has been significantly altered by anthropogenic forcings. However, how individual anthropogenic forcing affects the ET over monsoonal Asia is still elusive. In this study, we found a significant decline in ET over the Asian monsoon region during the period of 1950–2014 in Coupled Model Intercomparison Project Phase 6 (CMIP6) models. The attribution analysis suggests that anthropogenic aerosol forcing is the primary cause of the weakening in ET in the historical simulation, while it is only partially compensated by the strengthening effect from GHGs, although GHGs are the dominant forcings for surface temperature increase. The physical mechanisms responsible for ET changes are different between aerosol and GHG forcings. The increase in aerosol emissions enhances the reflection and scattering of the downward solar radiation, which decreases the net surface irradiance for ET. GHGs, on the one hand, increase the moisture capability of the atmosphere and, thus, the ensuing rainfall; on the other hand, they increase the ascending motion over the Indian subcontinent, leading to an increase in rainfall. Both processes are beneficial for an ET increase. The results from this study suggest that future changes in the land–water cycle may mainly rely on the aerosol emission policy rather than the carbon reduction policy.

Seaweed fertilizer, formulated primarily with seaweed extract as its main ingredient, has been extensively studied and found to significantly improve nutrient use efficiency, increase crop yield and quality, and enhance soil properties under field conditions. This growing body of evidence shows that seaweed fertilizer is a suitable option for sustainable agriculture in China. However, a comprehensive and quantitative analysis of the overall effects of seaweed fertilizer application in China is lacking. To address this gap, we conducted a meta-analysis of relevant studies on the effects of seaweed fertilizers under field conditions in China with MetaWin and SPSS software. Our analysis examined the effects of seaweed fertilizers on crop yield, quality, and growth under different preparation methods, application techniques, and regions. Our results showed that the application of seaweed fertilizer led to a significant average increase in crop yield of 15.17% compared with the control treatments. Root & tuber crops exhibited the most pronounced response, with a yield boost of 21.19%. Moreover, seaweed fertilizer application significantly improved crop quality, with elevations in the sugar-acid ratio (38.32%) vitamin C (18.07%), starch (19.65%), and protein (11.45%). In addition, plant growth parameters such as height, stem thickness, root weight, and leaf area showed significant enhancement with seaweed fertilizer use. The yield-increasing effect of seaweed fertilizers varied depending on their preparation and use method, climate, and soil of application location. Our study provides fundamental reference data for the efficient and scientific application of seaweed fertilizers in agricultural practices.

The role of focal packages in packaging ecosystems is crucial for the development of the entire ecosystem, as they are the packages on which other packages depend. However, the evolution of dependency groups in packaging ecosystems has not been systematically investigated. In this study, we examine the downstream dependency package groups (DDGs) in three typical packaging ecosystems—Cargo for Rust, Comprehensive Perl Archive Network for Perl, and RubyGems for Ruby—to identify their features and evolution. We also identify and analyze a special type of DDG, the collaborative downstream dependency package group (CDDG), which requires shared contributors. Our findings show that the overall development of DDGs, particularly CDDGs, is consistent with the status of the whole ecosystem, and the size of DDGs and CDDGs follows a power law distribution. Furthermore, the interaction mechanisms between focal packages and downstream packages differ between ecosystems, but focal packages always play a leading role in the development of DDGs and CDDGs. Finally, we investigate predictive models for the development of CDDGs in the next stage based on their features, and our results show that random forest and Gradient Boosting Regression Tree achieve acceptable prediction accuracy. We provide the raw data and scripts used for our analysis at https://github.com/onion616/DDG.

Testicular germ cell tumors (TGCT) are the most common reproductive system malignancies in men aged 15–44 years, accounting for 95 % of all testicular tumors. Our previous studies have been shown that long non-coding RNAs (lncRNAs), such as LINC00313, TTTY14 and RFPL3S, were associated with development of TGCT. Subgrouping TGCT according to differential expressed lncRNAs and immunological characteristics is helpful to comprehensively describe the characteristics of TGCT and implement precise treatment. In this study, the TGCT transcriptome data in The Cancer Genome Atlas Program (TCGA) database was used to perform consensus clustering analysis to construct a prognostic model for TGCT. TGCT was divided into 3 subtypes C1, C2, and C3 based on the differentially expressed lncRNAs. C1 subtype was sensitive to chemotherapy drugs, while the C2 subtype was not sensitive to chemotherapy drugs, and C3 subtype may benefit from immunotherapy. We defined the C1 subtype as epidermal progression subtype, the C2 subtype as mesenchymal progression subtype, and the C3 subtype as T cell activation subtype. Subgrouping based on differentially expressed genes (DEGs) and immunological characteristics is helpful for the precise treatment of TGCT.

Oil sources and accumulations in complex oil reservoirs of superimposed basins are difficult to identify and recover because of multiple kitchens and multistage charging processes. Constraining the oil sources and characterizing their accumulation patterns is a critical challenge. In this study, 46 crude oils from the Upper Wuerhe Formation, a widely distributed oil reservoir in the Mahu Sag of the Junggar Basin, NW China, are geochemically characterized to assess their sources and spatial distribution. These oils are divided into four groups: group I oils (characterized by high β-carotane and gammacerane concentrations, low C 21 /C 23 and (C 19 + C 20 )/C 23 tricyclic terpane ratios, and low Pr/Ph ratios); group II oils (characterized by low β-carotane and gammacerane concentrations, high C 21 /C 23 and (C 19 + C 20 )/C 23 tricyclic terpane ratios, and moderate Pr/Ph ratios); group III oils (very high Pr/Ph ratio (>3), the lowest β-carotane and gammacerane concentrations, and low tricyclic terpane concentration, but relatively high C 19 and C 20 tricyclic terpane abundance); and group IV oils (refered to here as geochemically hybrid, and inferred to be sourced from mixed P 1 f and C/P 1 j source rocks). The integration of oil-source correlation and geological framework indicates that oils generated from multiple kitchens are more likely to be near-sourced accumulations, providing a plausible example for oil accumulation in a complex oil reservoir of superimposed basin with several sets of kitchens and multistage oil charging history. The results show that multisource and multistage hydrocarbon accumulation is a common characteristic of prolific superimposed basins. The oil accumulation pattern must be established by carefully characterizing sources and charging processes to reduce the risk of exploration activities.

ABSTRACTThe psychological factors of senior executives have an important impact on the firm’s financial policies. In this paper, we investigate the association between CEO risk-taking entrepreneurial spirit and firm cash holdings, and find that firms with higher CEO risk-taking entrepreneurial spirit have less cash holdings. Further, we find that financing constraints can mitigate the negative association between CEO risk-taking entrepreneurial spirit and firm cash holdings, whereas financing needs enhances the association between them. Mechanism test illustrates that CEO risk-taking entrepreneurial spirit affects firm cash holdings through corporate investment. Finally, the results reveal that CEO risk-taking entrepreneurial spirit not only promotes the value of cash holdings, but also is significantly positively associated with both accounting performance and market performance. Our findings shed lights on the impact of executive psychological factors on financial decisions and have important implications for cash-holding decisions of listed firms.

Session-based recommender systems are increasingly applied to next-item recommendations. However, existing approaches encode the session information of each user independently and do not consider the interrelationship between users. This work is based on the intuition that dynamic groups of like-minded users exist over time. By considering the impact of latent user groups, we can learn a user’s preference in a better way. To this end, we propose a recommendation model based on learning user embeddings by modeling long and short-term dynamic latent user groups. Specifically, we utilize two network units to learn users’ long and short-term sessions, respectively. Meanwhile, we employ two additional units to determine the affiliation of users with specific latent groups, followed by an aggregation of these latent group representations. Finally, user preference representations are shaped comprehensively by considering all these four aspects, based on an attention mechanism. Moreover, to avoid setting the number of groups manually, we further incorporate an adaptive learning unit to assess the necessity for creating a new group and learn the representation of emerging groups automatically. Extensive experiments prove our model outperforms multiple state-of-the-art methods in terms of Recall, mean average precision (mAP), and area under curve (AUC) metrics.

A high-strength and anti-intergranular corrosion (IGC) Al-6.8Zn-2.2 Mg-Sc-Zr alloy (AS alloy) was designed, and the effects of Sc addition and Cu removal on the microstructure, mechanical properties and corrosion behavior were systematically investigated. Based on transmission electron microscopy (TEM) and Kelvin probe force microscopy (KPFM) techniques, the underlying causes of IGC and exfoliation corrosion (EXCO) of the AS alloy were investigated, and the corrosion mechanism of AS alloy was finally obtained. The AS alloy exhibits excellent mechanical properties with a tensile strength of 601 MPa in the peak-ageing condition at 120 °C, which comes from the pinning effect of Al3(Sc, Zr) dispersions. Moreover, AS alloys of T6 and T74 conditions show outstanding IGC and EXCO resistance. On the one hand, the fine, near-ellipsoidal grains in AS alloy alleviate the peeling stress caused by IGC products by blocking the formation of wedge-shaped gaps along GBs, which then bring about improved EXCO resistance. On the other hand, the removal of Cu from the traditional Al-Zn-Mg-Cu alloy avoids the segregation of Cu into GBs and thereafter reduces the fluctuations of the potential and the electron work function (EWF) across the GBs; the addition of Sc induces the high fraction of LAGBs and thus weakens the solutes' segregation at GBs, which can be beneficial for smoothing the fluctuation of corrosion potential (Ecorr) to enhance the IGC resistance.

Hydrogenation and nanocrystallization of Ti6Al4V alloy were achieved by electrolytic hydrogenation and SMAT processing. The hydride δ phase appeared when the hydrogenation time reached 4 h. The grain size of Ti6Al4V alloy after surface nanocrystallization reached below 35 nm when the SMAT processing time was 2 h. Surface morphology and roughness were characterized by AFM. The diffusion bonding between Ti6Al4V alloys at low temperature was achieved under different parameters. It is demonstrated that the porosity at the interface decreased correspondingly when the hydrogenation time or nanocrystallization time was extended. The porosity of the joint reached 0 when the hydrogenation time was 24 h or the nanocrystallization time was 4 h. Then hydrogenation and nanocrystallization treatments were combined. It is found that there are more bonding defects in both hydrogenation-nanocrystallization and nanocrystallization-hydrogenation joints than in joints with single hydrogenation or nanocrystallization treatment. It can be concluded that the porosity of the joints with nanocrystallization first is lower than that with hydrogenation first.

Abstract Objectives The purpose of this study was to analyze mid- to long-term outcomes of total hip arthroplasty (THA) following failed internal fixation of femoral neck fracture. Methods This study retrospectively analyzed 345 patients with femoral neck fracture who underwent THA after failure of internal fixation at our hospital between January, 2003 and December, 2019. Patients older than 55 years (n = 175) and patients no older than 55 years (n = 170) were compared in terms of complications and survival rates during follow-up, which lasted a mean of 6 years. Results The two age groups showed similarly low incidence of complications and similarly long periods of survival without revision surgery. Only three younger patients and two older patients underwent revision surgery during follow-up. The two groups showed similarly high survival rates at the end of follow-up (> 93%). Younger patients showed significantly bettter Harris hip score at last follow-up (90.2 vs. 88.1 points, p

A finite element method (FEM) combined with subroutines was established and used for tracing the evolution of stresses in M50 steel in quenching. The constitutive relation and thermal physical properties of M50 steel were tested and integrated into the modeling. The finite element analysis, taking into account of thermal stresses and martensitic phase transformation, predicts accurately the high-velocity nitrogen (HNQ) induced residual stresses in the M50 steel bulk. The simulation results suggested that the residual stresses in M50 steel is compressive at the surface while tensive at the center, along with very strong residual stresses at edges. The residual stress reaches to the high level of −810.3 MPa and −1436 MPa in the case of HNQ and water quenching, respectively. The evolution of stress is found to be jointly driven by the thermal stress and phase transformation stress. The thermal stress dominates the evolution at the initial stage while the phase transformation stress becomes dominating once the martensite transformation begins.

This paper proposes a fusion algorithm based on state-tracking collision detection and the simulated annealing potential field (SCD-SAPF) to address the challenges of obstacle avoidance for autonomous underwater vehicles (AUVs) in dynamic environments. Navigating AUVs in complex underwater environments requires robust autonomous obstacle avoidance capabilities. The SCD-SAPF algorithm aims to accurately assess collision risks and efficiently plan avoidance trajectories. The algorithm introduces an SCD model for proactive collision risk assessment, predicting collision risks between AUVs and dynamic obstacles. Additionally, it proposes a simulated annealing (SA) algorithm to optimize trajectory planning in a simulated annealing potential field (SAPF), integrating the SCD model with the SAPF algorithm to guide AUVs in obstacle avoidance by generating optimal heading and velocity outputs. Extensive simulation experiments demonstrate the effectiveness and robustness of the algorithm in various dynamic scenarios, enabling the early avoidance of dynamic obstacles and outperforming traditional methods. This research provides an accurate collision risk assessment and efficient obstacle avoidance trajectory planning, offering an innovative approach to the field of underwater robotics and supporting the enhancement of AUV autonomy and reliability in practical applications.

Two coupled climate models that participated in the CMIP6 project (Coupled Model Intercomparison Project Phase 6), the Earth System Model of Chinese Academy of Sciences version 2 (CAS-ESM2-0), and the Nanjing University of Information Science and Technology Earth System Model version 3 (NESM3) were assessed in terms of the impact of four new sea ice parameterization schemes. These four new schemes are related to air–ice heat flux, radiation penetration and absorption, melt ponds, and ice–ocean flux, respectively. To evaluate the effectiveness of these schemes, key sea ice variables with and without these new schemes, such as sea ice concentration (SIC) and sea ice thickness (SIT), were compared against observation and reanalysis products from 1980 to 2014. The simulations followed the design of historical experiments within the CMIP6 framework. The results revealed that both models demonstrated improvements in simulating Arctic SIC and SIT when the new parameterization schemes were implemented. The model bias of SIC in some marginal sea ice zones of the Arctic was reduced, especially during March. The SIT was increased and the transpolar gradient of SIT was reproduced. The changes in spatial patterns of SIC and SIT after adding new schemes bear similarities between the two coupled models. This suggests that the new schemes have the potential for broad application in climate models for simulation and future climate scenario projection, especially for those with underestimated SIT.

This work investigates the inter-model diversity of the Pacific Decadal Oscillation’s (PDO) impact on tropical cyclone frequency (TCF) over the Western North Pacific (WNP) from the historical simulation of twenty-two Coupled Model Intercomparison Project Phase 6 (CMIP6) models. The impact of the PDO is expressed as the TCF difference between the positive and negative PDO phases. The comparison between the models with high PDO skill and low PDO skill shows that the PDO-related sea surface temperature (SST) gradient between the western and central tropical Pacific plays an important role in changing the large-scale atmospheric dynamic fields for TC genesis and, thus, the TCF over the WNP. This SST gradient also significantly contributes to the inter-model spread of PDO’s impact on TCF across the 22 CMIP6 models. We, therefore, stress that the PDO-related eastward SST gradient between the western and central tropical Pacific triggers the lower troposphere westerly and eastward extending of the monsoon trough over the WNP. The moistening of the atmosphere and enhancing ascending motion in the mid-troposphere promote convection, leading to the easterly wind anomaly over the upper troposphere, which reduces the vertical wind shear. Those favorable dynamic conditions consistently promote the TC formation over the southeastern part of the Western North Pacific. Our results highlight that PDO could impact the WNP TCF through its associated tropical SST gradient.

Abstract Background CircRNAs are involved in the pathogenesis of several central nervous system diseases. However, their functions and mechanisms in spinal cord injury (SCI) are still unclear. Therefore, the purpose of this study was to evaluate circRNA and mRNA expression profiles in the pathological setting of SCI and to predict the potential function of circRNA through bioinformatics. Methods A microarray-based approach was used for the simultaneous measurement of circRNAs and mRNAs, together with qPCR, fluorescence in situ hybridization, western immunoblotting, and dual-luciferase reporter assays to investigate the associated regulatory mechanisms in a rat SCI model. Results SCI was found to be associated with the differential expression of 414 and 5337 circRNAs and mRNAs, respectively. Pathway enrichment analyses were used to predict the primary function of these circRNAs and mRNAs. GSEA analysis showed that differentially expressed mRNAs were primarily associated with inflammatory immune response activity. Further screening of these inflammation-associated genes was used to construct and analyze a competing endogenous RNA network. RNO_CIRCpedia_4214 was knocked down in vitro, resulting in reduced expression of Msr1, while the expression of RNO-miR-667-5p and Arg1 was increased. Dual-luciferase assays demonstrated that RNO_CIRCpedia_4214 bound to RNO-miR-667-5p. The RNO_CIRCpedia_4214/RNO-miR-667-5p/Msr1 axis may be a potential ceRNA that promotes macrophage M2-like polarization in SCI. Conclusion Overall, these results highlighted the critical role that circRNAs may play in the pathophysiology of SCI and the discovery of a potential ceRNA mechanism based on novel circRNAs that regulates macrophage polarization, providing new targets for the treatment of SCI.

Staplers have been widely used in the clinical treatment of gastrointestinal reconstruction. However, the current titanium (Ti) staple will remain in the human body permanently, resulting in some adverse effects. In this study, we developed a type of biodegradable staple for colonic anastomosis using 0.3 mm diameter magnesium (Mg) alloy wires. The wire surface was modified by micro-arc oxidation treatment (MAO) and then coated with poly-l-lactic acid (PLLA) to achieve a moderate degradation rate matching the tissue healing process. The results of tensile tests on isolated porcine colon tissue anastomosed by Mg and Ti staples showed that the anastomotic property of Mg staples was almost equal to that of Ti staples. The in vitro degradation tests indicated the dual-layer coating effectively enhanced the corrosion resistance and maintained the tensile force of the coated staple stable after 14-day immersion in the simulated colonic fluid (SCF). Furthermore, 24 beagle dogs were employed to conduct a comparison experiment using Mg-based and clinical Ti staples for 90-day implantation by ent-to-side anastomosis of the colon. The integrated structure of Mg-based staples was observed after 7 days and completely degraded after 90 days. All animals did not have anastomotic leakage and stenosis, and 12 dogs with Mg-based staples fully recovered after 90 days without differences in visceral ion levels and other side effects. The favorable performance makes this Mg-based anastomotic staple an ideal candidate for colon reconstruction.

The diffusion of Sulfate ions in concrete is a complex process and affects the performance of concrete. Experiments on the time-dependent distribution of sulfate ions in concrete under the coupling of pressure load, dry-wet circulation, and sulfate attack, and the diffusion coefficient of sulfate ions with various parameters was tested. The applicability of the cellular automata (CA) theory to simulate the diffusion of sulfate ions was discussed. In this paper, a multiparameter cellular automata (MPCA) model was developed to simulate the impacts of load, immersion ways, and sulfate solution concentration for the diffusion of sulfate ions in concrete. The MPCA model was compared with experimental data, considering compressive stress, sulfate solution concentration, and other parameters. The numerical simulations verify the calculation results based on the MPCA model are in good agreement with the test data. Finally, the applicability of the established MPCA model was also discussed.

Abstract Background Patients with rheumatoid arthritis (RA) undergoing bilateral total hip arthroplasty (THA) and total knee arthroplasty (TKA) are an uncommon population, and their outcomes are also difficult to predict. The purpose of this study was to evaluate whether both bilateral cementless THA and cemented posterior-stabilized TKA (PS-TKA) can provide reliable outcomes for RA patients. Methods We retrospectively reviewed 30 RA patients (60 hips and 60 knees) who underwent both elective bilateral cementless THA and cemented PS-TKA, with a minimum follow-up of 2 years. Clinical, patient-reported, and radiographic data were retrospectively analyzed. Results The mean follow-up was 84 months (range, 24–156). By the last follow-up, the post-operative range of motion, Harris Hip Score, Knee Society Score (KSS) clinical, KSS functional, Western Ontario and McMaster Universities Index of Osteoarthritis (WOMAC) hip, and WOMAC knee scores were significantly improved compared to the preoperative values. All patients achieved the ability to walk. In addition, overall satisfaction scores on a 100-point scale were 92.5 after THA and 89.6 after TKA. Only one patient underwent revision surgery due to knee joint instability, and all replaced hips and knees were radiographically stable by the assessment of the radiolucent line. The proportion of implants that did not suffer loosening or require revision surgery was 99.2% during the 84-month follow-up, based on Kaplan-Meier analysis. Conclusions Our study suggests that bilateral cementless THA and cemented PS-TKA provides reliable mid-long-term clinical, patient-reported, and radiographic outcomes in RA patients, with high survivorship and patient satisfaction.

Abstract Analyses of several databases showed that the lncRNA RNF157 Antisense RNA 1 (RNF157-AS1) is overexpressed in epithelial ovarian cancer (EOC) tissues. In our study, suppressing RNF157-AS1 strikingly reduced the proliferation, invasion, and migration of EOC cells compared with control cells, while overexpressing RNF157-AS1 greatly increased these effects. By RNA pulldown assays, RNA binding protein immunoprecipitation (RIP) assays, and mass spectrometry, RNF157-AS1 was further found to be able to bind to the HMGA1 and EZH2 proteins. Chromatin immunoprecipitation (ChIP) assays showed that RNF157-AS1 and HMGA1 bound to the ULK1 promoter and prevented the expression of ULK1. Additionally, RNF157-AS1 interacted with EZH2 to bind to the DIRAS3 promoter and diminish DIRAS3 expression. ULK1 and DIRAS3 were found to be essential for autophagy. Combination autophagy inhibitor and RNF157-AS1 overexpression or knockdown, a change in the LC3 II/I ratio was found using immunofluorescence (IF) staining and western blot (WB) analysis. The autophagy level also was confirmed by autophagy/cytotoxicity dual staining. However, the majority of advanced EOC patients require platinum-based chemotherapy, since autophagy is a cellular catabolic response to cell stress. As a result, RNF157-AS1 increased EOC cell sensitivity to chemotherapy and death under cis-platinum (DDP) treatment by suppressing autophagy, as confirmed by cell count Kit-8 (CCK8) assays, flow cytometry, and autophagy/cytotoxicity dual staining. Therefore, the OS and PPS times were longer in EOC patients with elevated RNF157-AS1 expression. RNF157-AS1-mediated autophagy has potential clinical significance in DDP chemotherapy for EOC patients.

Additive friction stir deposition (AFSD) is a relatively new additive manufacturing technique to fabricate parts in solid state below the melting temperature. Due to the solid-state nature, AFSD benefits from lower residual stresses as well as significantly lower susceptibility to porosity, hot-cracking, and other defects compared to conventional fusion-based metallic additive manufacturing. These unique features make AFSD a promising alternative to conventional forging for fabricating large structures for aerospace, naval, nuclear, and automotive applications. This review comprehensively summarizes the advances in AFSD, as well as the microstructure and properties of the final part. Given the rapidly growing research in AFSD, we focus on fundamental questions and issues, with a particular emphasis on the underlying relationship between AFSD-based processing, microstructure, and mechanical properties. The implications of the experimental and modeling research in AFSD will be discussed in detail. Unlike the columnar structure in fusion-based additive manufacturing, fully dense material with a fine, fully-equiaxed microstructure can be fabricated in AFSD. The as-wrought structure brings the as-printed parts with comparable properties to wrought parts. The fundamental difference between AFSD and fusion-based metallic additive manufacturing will be summarized. Furthermore, the existing challenges and possible future research directions are explored.

The effects of different test parameters on the creep of axially compressed concrete members were investigated under standard test conditions, considering loading ages of 7, 14 and 28 days and mineral powder admixtures of 0, 15%, 25% and 35%, respectively. The test results show that the creep of concrete decreases with the increase of mineral powder content when the loading age was fixed. When the content of mineral powder was unchanged, the creep of concrete would decrease with the increase of loading age. Based on the CEB-FIP MC 1990 concrete creep calculation model, the creep correction coefficient of mineral powder concrete considering the strength of mineral powder concrete, mineral powder content and loading age was proposed. The formula for the concrete creep correction coefficient which considers the loading age and mineral powder content was established by combining the existing creep test data and the test results of mineral powder concrete strength with the different loading ages and mineral powder contents in this paper. The comparison of the CEB-FIP MC 1978 and experiment values shows that the proposed formula in this paper can predict the development of creep of mineral powder concrete better. Finally, using the creep coefficient formula of mineral powder concrete, the creep coefficient values of concrete under different loading ages and mineral powder contents in CEB-FIP MC 1990 code were modified.

Abstract Immunotherapy is now the main choice of systemic therapy for many cancer patients, while current biomarkers for tumor immunotherapy may be limited by the accessibility of patient tumor tissue or tumor neoplastic content. Rare mutation in the 5’ and 3’-untranslated region (UTR) of CD274 gene (Protein name: PD-L1) has been recently reported in hematologic and solid tumors as a potential biomarker for assessing efficacy during immunotherapy. However, multi-omics analysis for CD274 UTR region, especially circulating tumor DNA (ctDNA), have been little explored in the pan-cancer perspective. We developed a cSMART2.0 technology featured with higher capture efficiency and homogeneity to detect this rare structural variant in 2249 Chinese patients’ cohort with multiple cancers. An incidence of 0.36% was detected in this cohort, consistent with TCGA (The Cancer Genome Atlas), while the prevalence of SV in CD274 UTR region in liver and breast cancer were significantly higher than TCGA. The liquid biopsy result from ctDNA was 100% concordance with gDNA result getting from tumor tissue detection, and further validated by immunohistochemistry (IHC) and multiplex immunofluorescence (mIF) experiments. Patients carrying this SV in CD274 UTR region without driver gene mutation responded to immune checkpoint inhibitors (ICIs). This study proves that rare structural variants in CD274 UTR region exist in various cancer in Chinese population for the first time, which can induce immune escape and be used for prediction of response to ICIs. Liquid biopsy based cSMART 2.0 technology could offer more sensitive and accurate detection to navigate potential ICIs patients and to benefit patients with advanced disease when tissue samples are not available.

Abstract Tumor mutational burden (TMB) is an emerging predictive marker of response to immune checkpoint inhibitor therapies. We evaluated the correlation between clinical indicators and high‐throughput sequencing results and TMB in lung adenocarcinoma patients, with the aim of finding simpler and more economical factors as surrogate markers for TMB. The medical records, next‐generation sequencing data, and immunohistochemistry results of 340 lung adenocarcinoma patients who were admitted to the First Affiliated Hospital of Zhengzhou University between 2019 and 2020 were collected. The mutated genes were screened for, and the obtained mutated genes were subjected to functional enrichment analysis using R software. A protein–protein interaction (PPI) network was also constructed, and significant modules in the network were identified. Gene Ontology (GO) analyses were performed for the core genes. Univariate and multivariate correlation analyses were performed to judge the correlation between gene mutations and TMB. Genes with a junction mutation rate >1 were selected to construct PPI network and 13 high‐connection core genes were screened. The results of GO enrichment analysis showed that the biological processes related to mutant core genes mainly included mitotic cell cycle and cell aging. Subsequently, ATM (p = 0.006) and PIK3CA (p = 0.008) mutation positivity were identified by univariate and multivariate correlation analysis, while TP53 (p = 0.003) and EGFR (p = 0.008) mutation negativity were significantly associated with elevated TMB. The results of this study demonstrate that ATM‐ and PIK3CA‐positive and EGFR‐negative mutation status are strongly associated with high levels of TMB and have the potential to be predictive biomarkers of response to immune checkpoint inhibitors in lung adenocarcinoma patients.

The Pacific Decadal Oscillation (PDO) and Pacific Meridional Mode (PMM) are prominent climate modes in the North Pacific with well-established impacts on tropical cyclone (TC) genesis in the western North Pacific (WNP) basin. While previous research has primarily focused on the roles of the PDO and PMM in regulating TC genesis through the modification of large-scale environmental factors, this study investigates the evolving influence of the PDO on WNP TC genesis since the 1950s. Remarkably, our analysis reveals a shift in the PDO-TC genesis relationship, transitioning from a significant negative correlation to a significant positive correlation since the 1990s. This shift is attributed to variations in the specific large-scale factors through which the PDO affects TC genesis. Furthermore, this study suggests that these changes appear to be linked to the PMM strengthening on the interdecadal timescale in recent decades. The linkage of the PMM strengthening to the PDO-related atmospheric circulation is further confirmed by the results of a 500 year pre-industrial numerical experiment, suggesting that the PMM strengthening may result from natural internal variability. The results underscore the non-stationary relationship between PDO and WNP TC genesis, with the PMM intensity probably influencing their relationship.

This paper presents an improved motion controller based on the backstepping method to address nonlinear control challenges in unmanned aerial–aquatic vehicles (UAAVs), enabling them to navigate between two different media. The nonlinear control approach is applied to UAAV motion control, incorporating filters to improve stability. The study designs motion controllers for three UAAV phases: underwater, in the air, and transitioning between media. Fluid simulations of the emergence process of the UAAV for future field experiments were conducted. By fine-tuning the simulations, a comprehensive understanding of the vehicle’s performance is obtained, offering crucial insights for the development of subsequent control systems. Simulation results confirm the controller’s ability to achieve target trajectory tracking with control system responses that meet practical requirements. The controller’s performance in attitude control and trajectory tracking is verified in underwater gliding, transmedia transitions, and airborne phases, demonstrating its effectiveness.

This article explores trajectory-following control for an unmanned aerial–aquatic vehicle (UAAV) navigating complex ocean disturbances and the interplay of air–seawater coupling factors. First, leveraging the backstepping technology, an adaptive algorithm is proposed to tackle the attitude and position following. Additionally, a nonlinear observer is crafted to estimate complex ocean disturbances. The UAAV model, characterized by six degrees of freedom (DOF) and nonlinear properties, experiences significant pose changes when emerging from water, underscoring the critical importance of precise pose control. Finally, stability analysis and numerical simulations are demonstrated to verify the feasibility and validity of the proposed control strategies.

The northwestern Junggar Basin in the southwestern Central Asian Orogenic Belt is a typical petroliferous basin. The widely distributed reservoirs in Jurassic–Cretaceous strata indicate that the region records Yanshanian–Himalayan tectonic activity, which affected the accumulation and distribution of petroleum. The mechanism of this effect, however, has not been fully explored. To fill the knowledge gap, we studied the structural geology and geochemistry of the well-exposed Wuerhe bitumen deposit. Our results indicate that deformation and hydrocarbon accumulation in the northwestern Junggar Basin during the Yanshanian–Himalayan geodynamic transformation involved two main stages. During the Yanshanian orogeny, a high-angle extensional fault system formed in Jurassic–Cretaceous strata at intermediate to shallow depths owing to dextral shear deformation in the orogenic belt. This fault system connected at depth with the Permian–Triassic oil–gas system, resulting in oil ascending to form fault-controlled reservoirs (e.g., a veined bitumen deposit). During the Himalayan orogeny, this fault system was deactivated owing to sinistral shear caused by far-field stress related to uplift of the Tibetan Plateau. This and the reservoir densification caused by cementation formed favorable hydrocarbon preservation and accumulation conditions. Therefore, the secondary oil reservoirs that formed during the Yanshanian–Himalayan tectonic transformation and the primary oil reservoirs that formed during Hercynian–Indosinian orogenies form a total and complex petroleum system comprising conventional and unconventional petroleum reservoirs. This might be a common feature of oil–gas accumulation in the Central Asian Orogenic Belt and highlights the potential for petroleum exploration at intermediate–shallow depths.

BackgroundTraumatic lumbosacral spondyloptosis is a very rare spinal disease caused by high-energy trauma. We report a case of traumatic lumbosacral spondyloptosis with locked L5 inferior articular process.Case presentationA 33-year-old man presented with multisite pain for 6 h following waist trauma and was admitted to the hospital. He suffered multiple injuries from severe impact on the waist after driving an out of control forklift truck. Preoperative imaging examinations revealed that the patient was diagnosed with traumatic lumbosacral spondyloptosis and the L5 inferior articular process was locked into the anterior margin of the S1 vertebra. A posterior instrumentation, decompression of the cauda equina, and interbody fusion procedure was performed. The patient received hyperbaric oxygen and rehabilitation treatment 10 days after the surgery. At the 6-month postoperative follow-up, the muscle strength of the lower limbs was improved, the patient had no numbness of both lower limbs, and the urinary retention symptom was significantly improved. The American Spinal Injury Association grade improved from grade C preoperatively to grade D postoperatively. As far as we know, there have been no relevant reports on traumatic lumbosacral spondyloptosis with locked L5 inferior articular process yet.ConclusionWe believe that the hyperflexion and shear forces were the potential causes of this injury. In addition, the preoperative imaging examinations should be evaluated carefully. If the inferior articular process of L5 were locked, we suggest removing the bilateral inferior articular processes first and then perform reduction.

Objectives: Long-term use of evidence-based antiplatelet therapy is recommended for management of stable coronary artery disease (SCAD). However, non-adherence to antiplatelet drugs is common in older patients. This study aimed to evaluate the incidence and impact of antiplatelet therapy cessation on clinical outcomes of older patients with SCAD.Methods: A total of 351 consecutive eligible very older patients (≥80 years) with SCAD from the PLA General Hospital were included. Baseline demographics, clinical characteristics, and clinical outcomes were collected during follow-up. Patients were divided into cessation group and standard group based on whether discontinuing of antiplatelet drugs. The primary outcome was major adverse cardiovascular events (MACE) and secondary outcomes were minor bleeding and all-cause mortality.Results: A total of 351 participants, with a mean age of 91.76 ± 5.01 years old (range 80–106 years) were included in statistical analysis. The antiplatelet drug cessation rate was 60.1%. There were 211 patients in cessation group and 140 patients in standard group. During a median follow-up of 98.6 months, the primary outcome of MACE occurred in 155 patients (73.5%) in the cessation group and 84 patients (60.0%) in the standard group (HR = 1.476, 95% CI:1.124-1.938, p = 0.005). Cessation of antiplatelet drugs increased the rates of angina (HR = 1.724, 95% CI:1.211-2.453, p = 0.002) and non-fatal MI (HR = 1.569, 95% CI:1.093-2.251, p = 0.014). The secondary outcomes of minor bleeding and all-cause mortality were similar between the two groups.Conclusion: Among very older patients with SCAD, antiplatelet therapy cessation significantly increased the risk of MACE, and continuous antiplatelet drug therapy didn’t increase the risk of minor bleeding.