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This paper explores how significant are the ecological and climatic variables to influence the stable isotopes of guanacos. Lama guanicoe bone collagen carbon and nitrogen stable isotope ratios are assumed as a macro regional average value in west Argentina, mostly as a baseline to model archaeological human diet. If stable isotopes on mammals reflex ecology and climate, we need to know how those variables influence mammals bone stable isotope ratio. This paper analyses the 13C/12C and 15 N/14N ratio on bone collagen on 122 guanacos from Northwest Patagonia during the Holocene. The results confirm significant variation in both isotopes between Monte and Andean-Patagonian specimens. Guanacos from Monte shows higher δ13C and δ15N than those from Andean-Patagonian. Temporal trends indicate variation through Holocene but this variation is not spatially homogeneous. In this paper we suggest that Medieval Climatic Anomaly had stronger effect in Monte desert than in Patagonia desert, generating driest and/or hottest conditions between 1250 and 600 years BP. Those variations need to be considered to reconstruct human diet at least during the second part of the Holocene. [ABSTRACT FROM AUTHOR]