1. Identificating 14-3-3 sigma as a lymph node metastasis-related protein in human lung squamous carcinoma
- Author
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Li, Dan-juan, Deng, Gui, Xiao, Zhi-qiang, Yao, Hui-xin, Li, Cui, Peng, Fang, Li, Mao-yu, Zhang, Peng-fei, Chen, Yong-heng, and Chen, Zhu-chu
- Subjects
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LYMPHATIC metastasis , *THERAPEUTIC use of proteins , *LUNG cancer , *LUNG surgery , *MICRODISSECTION , *GENE expression , *WESTERN immunoblotting , *IMMUNOHISTOCHEMISTRY - Abstract
Abstract: In this study, we aim to screen metastasis-related proteins in human lung squamous carcinoma (LSC) using laser capture microdissection and a proteomic approach. Twenty two differential proteins were identified from pooled microdissected primary LSC and matched lymph node (LN) metastatic tissues. Expression of the differential protein 14-3-3 sigma was determined by Western blotting and immunohistochemistry. In cell invasion assay, down-regulated 14-3-3 sigma by siRNA increased in vitro invasive ability of HTB-182 and A549 cells, up-regulation of 14-3-3 sigma by pcDNA3.0/14-3-3 sigma decreased in vitro invasive ability of HTB-182 and A549 cells. The data suggest that 14-3-3 sigma is a potential LN metastasis-related protein in LSC, and its dysregulation might play an important role in the LN metastatic process of LSC. [Copyright &y& Elsevier]
- Published
- 2009
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