1. Single-cell RNA sequencing analysis to evaluate antimony exposure effects on cell-lineage communications within the Drosophila testicular niche.
- Author
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Cui, Hongliang, Huang, Qiuru, Li, Jiaxin, Zhou, Peiyao, Wang, Zihan, Cai, Jiaying, Feng, Chenrui, Deng, Xiaonan, Gu, Han, He, Xuxin, Tang, Juan, Wang, Xiaoke, Zhao, Xinyuan, Yu, Jun, and Chen, Xia
- Subjects
RNA sequencing ,STEM cell niches ,CELL communication ,EPIDERMAL growth factor receptors ,DROSOPHILA ,ANTIMONY - Abstract
The increasing production and prevalence of antimony (Sb)-related products raise concerns regarding its potential hazards to reproductive health. Upon environmental exposure, Sb reportedly induces testicular toxicity during spermatogenesis; moreover, it is known to affect various testicular cell populations, particularly germline stem cell populations. However, the cell-cell communication resulting from Sb exposure within the testicular niche remains poorly understood. To address this gap, herein we analyzed testicular single-cell RNA sequencing data from Sb-exposed Drosophila. Our findings revealed that the epidermal growth factor receptor (EGFR) and WNT signaling pathways were associated with the stem cell niche in Drosophila testes, which may disrupt the homeostasis of the testicular niche in Drosophila. Furthermore, we identified several ligand-receptor pairs, facilitating the elucidation of intercellular crosstalk involved in Sb-mediated reproductive toxicology. We employed scRNA-seq analysis and conducted functional verification to investigate the expression patterns of core downstream factors associated with EGFR and WNT signatures in the testes under the influence of Sb exposure. Altogether, our results shed light on the potential mechanisms of Sb exposure-mediated testicular cell-lineage communications. [Display omitted] • Antimony exposure altered intercellular communication networks in Drosophila. • Number of enriched ligand-receptor pairs was reduced among germline stem cell niche. • EGFR signaling was weakened, affecting soma-soma and soma-germline interactions. • WNT signaling was inhibited, causing germline stem cell niche disruption. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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