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Silver nanoparticles (AgNP) can help prevent infection of virus and bacteria. The size and morphology of AgNP can be crucial to function, with smaller nanoparticles (< 20 nm) able to penetrate the cell wall. This is significant as oxidative stress and genotoxicity are associated with some sizes and coatings of AgNP, contraindicating the use of AgNP to reduce infection. We present evidence that a microfluidic chip can synthesize larger sizes and distributions of AgNP from the nano-to-micro size range. We show results from a microfluidic mixing chip that can produce a wide range of nano-to-micro size (~ 24–400 nm) AgNP. Synthesis is based on a modified Turkevich method, using a single-step AgNP synthesis on the microfluidic chip using two chemical components, trisodium citrate (NaCit) and AgNO3. To make AgNP more accessible, we describe the microfluidic chip and conditions capable of synthesis. We also describe how modification of flow rate and chemical reagent concentration change particle diameter during production. In our experiments, we found that AgNP production created a visible adsorption line in the microfluidic device, possibly owing to AgNP surface interaction at the polydimethylsiloxane (PDMS) interface. We characterize these particles with dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Based on optical light microscopy, we hypothesize that AgNP formation primarily occurs at the interface between the two chemical reagent streams. We also conclude that AgNP size increases could be due to interaction with the PDMS surface, which is known to be porous. Future work will help to understand how surface interaction may influence the formation of larger particles. [ABSTRACT FROM AUTHOR]

Three types of silver/silver chloride nanoparticles were obtained by green synthesis from three types of microbial biomass. Their biocidal capacity was tested against six microorganisms. Two filamentous fungi were used that had previously demonstrated the ability to synthesise nanoparticles, Penicillium sp. 8L2 and Botryosphaeria rhodina MAMB-05. Also, the synthesis capacity of a yeast, Rhodotorula mucilaginosa 1S1, was evaluated. The original protocols underwent slight modifications. At the same time, the fractional inhibitory concentration was obtained. The interaction between specific antibiotics and the nanoparticles that showed the greatest biocidal capacity came from Penicillium sp.8L2, and it was studied further. All nanoparticles were characterised by UV–vis spectrophotometry and transmission electron microscopy (TEM). Also, their size distribution was analysed, which was in the range of 4 to 34 nm. The biocidal capacity of the nanoparticles for a group of bacteria and fungi was studied, presenting very low values in the range of 2.5–10 µg/mL for bacteria and 4–256 µg/mL for fungi. The interactions between the nanoparticles synthesised by Penicillium sp. 8L2 and a group of specific antibiotics for the tested microorganisms were also studied, proving that there was a synergistic interaction with vancomycin and ciprofloxacin and Staphylococcus epidermidis CECT 4183 and Escherichia coli CECT 101 bacteria, respectively. [ABSTRACT FROM AUTHOR]

Drug resistance is a major concern nowadays, and finding alternatives of the well-known antibiotic is necessary. Green nanoparticles are emerging as a tenable alternative to this with a large spectrum of activity. The present manuscript describes an eco-friendly approach for green synthesis of silver nanoparticles from both in vitro and in vivo leaf extract of Coleus forskohlii. Leaf extracts were used in synthesis of nanoparticles which were further analyzed through UV–Vis, dynamic light scattering, energy-dispersive spectroscopy, and transmission electron microscopy. Antimicrobial activity of silver nanoparticles alone, as well as crude extract of the plant itself, was carried out against eight multidrug-resistant respiratory tract infecting pathogenic strains. Satisfactory antimicrobial activities were found with nanoparticles, in vitro and in vivo leaf extracts. However, gradually higher to lower inhibition potential against pathogenic bacterial strains was found in silver nanoparticles, in vitro and in vivo leaf extracts. Seven bioactive compounds were detected in the crude extract through gas chromatography–mass spectroscopy analysis. Results revealed that nanoparticle formation occurred in a wide range of sizes (10–50 nm) and shapes (trigonal, hexagonal, spherical, rod). The diversity in size and shape of the nanoparticles makes them biologically active. Silver nanoparticle exhibits significantly better antimicrobial activities as compared to the plant extract in case of nearly all pathogens with a maximum zone of inhibition of 15.33 ± 0.94 mm where more than 12 well-known antibiotics failed to respond. Because of this broad-spectrum activity of nanoparticles as well as the leaf extracts against life-threatening microbes, it can be used as future generation drugs. [ABSTRACT FROM AUTHOR]

The silver nanoparticles (Ag-Nps) synthesized by green reduction process using the leaf extract of Acacia leucophloea exhibited the surface plasmon resonance (SPR) at 435 nm. There were several techniques like UV–visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and selected area electron diffraction patterns (SAED) used to examine the growth kinetics, surface morphology, and crystallinity aspects of Ag-Nps in the absence and the presence of cationic surfactant, cetyltrimethylammonium bromide (CTAB). Fourier transform infrared spectrometry (FTIR) was used to confirm the reducing utility of leaf extract. The size of synthesized Ag-Nps varied from 10 to > 100 nm in the absence of CTAB and from 10 to 50 nm in its presence. While irregular or polytope-outlined Ag-NPs were detected in the absence of CTAB, spherical or oval shape prevailed in its prevalence. Williamson-Hall models were employed to deduce the crystallinity aspects of the procured Ag-Nps. Furthermore, the Ag-Nps effected in the presence of CTAB exhibited more antibacterial efficacy. [ABSTRACT FROM AUTHOR]