1. Current methods in protein-protein interactions research
- Author
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Křivková, Jana, Hrdý, Ivan, and Kučerová, Jitka
- Subjects
proximitní značení ,affinity chromatography ,proximity labeling ,protein interactions ,cross-linking ,afinitní chromatografie ,biotinylation ,proteinové interakce ,biotinylace - Abstract
Protein-protein interactions (PPI) have a crucial role in all processes in living cells. Understanding the interactions between proteins allows us to describe cell processes in more detail and their study opens new possibilities for drug design. The importance of the question of studying PPI is shown in the recent development of various methods for their identification and description. The aim of this thesis is to give an overview of new and improved experimental methods of identification and characterization of protein-protein interactions. Methods described in this thesis are divided in four chapters - proximity-dependent labelling methods (BioID, BioID2, APEX, TurboID, MiniTurbo, PUP-IT, AirID, SPPLAT, EMARS), cross-linking methods (XS-MS), fluorescence methods for identification and visualization (BiFC, FRET, BRET) and biophysical methods for description of kinetics and thermodynamics parameters of interaction (SPR, ITC, MT).
- Published
- 2022