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Your search keyword '"cellular stress"' showing total 2 results
Start Over Descriptor "cellular stress" Language czech
2 results on '"cellular stress"'

1. Cryopreservation: The secret of modern preservation of brewer’s yeasts – minireview

Author

Katarína Hanzalíková, Petra Kubizniakova, Lucie Kyselová, and Dagmar Matoulková

Subjects
long-term storage of yeast, cryopreservation, cryoprotective substances, saccharomyces, brewer's yeasts, cellular stress, Fermentation industries. Beverages. Alcohol, TP500-660
Abstract

The aim of the long-term preservation of cells, tissues and organs is to maintain their cellular structures and biological functions for as long as possible. Cryopreservation is a process where biological material is stored and preserved at very low temperatures. However, freezing and thawing processes can cause irreversible cell damage, which is related to formation of ice crystals, osmotic stress, accumulation of reactive forms of oxygen, etc. Therefore the cell viability depends mainly on the freezing rate, the composition of the cryoprotective medium as well as on the thawing rate. Using a suitable cryoprotective medium can increase the viability rate of the yeasts after “revitalization“. Appropriate pre-cultivation before freezing also plays an important role. These facts show that cell freezing and thawing processes must be controlled to avoid cell damage.

Published
2021
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2. Analysis of cell signaling mediated by the adapter protein Daxx

Author

Švadlenka, Jan, Anděra, Ladislav, Forstová, Jitka, and Stopka, Tomáš

Subjects
Daxx, apoptóza, transcription, signalizace, cellular stress, interagující proteiny, transkripce, apoptosis, buněčný stres, interacting proteins, signaling
Abstract

2 Abstract Multifunctional adapter protein and histone chaperone Daxx has been described in nu- merous cellular processes, including the regulation of apoptotic and stress signalling, antiviral response and processes connected to chromatin (e. g. transcription). Its influ- ence on chromatin-related processes is mainly carried out by several associated en- zymes, such as DNA-methyltransferase-1, histone deacetylases and chromatin- remodelling ATPase ATRX. In the complex with ATRX Daxx functions as a chaperone of histone-3.3, maintaining the constitutive heterochromatin e. g. at centromeric and telomeric regions. The main aim of this Thesis was a better understanding of the Daxx cellular functions through identification and functional characterization of its novel interacting proteins. Using the yeast two-hybrid screen, several such new Daxx-interacting proteins were identified. These proteins were mainly nuclear, connected to the regulation of chroma- tin-related processes. More detailed analysis focused on the interaction of Daxx with chromatin-remodelling ATPase Brg1. This interaction was confirmed both in vitro and in the cells, where Daxx and Brg1 associated mainly in high molecular weight pro- tein complexes. These likely chromatin-remodelling complexes contain, in addition to Brg1, several...

Published
2016

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