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This bachelor thesis is focused on the use of alternative sources in a selected region. More specifically, it deals with biogas plants in a selected region. The thesis is divided into theoretical part and practical part. The theoretical part contains general information, such as types of biogas plants, their general usage, their strategies, from which they draw material for energy production and where they invest this energy. The practical part consists of an analysis of the activities of biogas plants in the selected region. This analysis works with specific data on activities, strategies of specific biogas plants, their drawing subsidies or their plans for the future. The analysis includes interviews with representatives of selected biogas plants, from which the results of the survey outcome.

This thesis is focused on the development of an analytical method for the determination of volatile fatty acids in aqueous samples using GC-MS/MS. The theoretical section provides general information on a selected group of acids, their significance in the biogas plants, the means of their derivatization and the analytical techniques used. The practical part mainly deals with optimizition of various parameters in the derivatization reaction to achieve optimal conditions. Finally, the analytical method was successfully validated and applied to the analysis of a real sample.