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In a group of 22 women with cervical intraepithelial neoplasia and carcinoma of the cervix uteri and in a control group of 37 healthy women, a number of anamnestic and objective data about factors influencing these women and about consequences of such influence were analysed. Statistically significant differences between patients and controls were found in the character of work performed (physical vs. intellectual, p = 0.0312), the presence of stressing factors at the workplace (p = 0.02), a higher number of consumed meals per day in the control group (p = 0.0009), daily consumption of beer in a higher percentage of patients (p = 0.0015), the frequency of sexual intercourse in the last 2-3 months before the diagnosis of disease or examination (higher in controls, p = 0.0164) and subjective rating of sexual life in the fourth decade (better rating in controls, p = 0.0005). No differences were found between patients and controls in the number of sexual partners, the presence of antibodies against HSV-2 and other factors. According to data presented and data in the literature, external environment, some of the characteristics of sexual life and particularly infection with certain types of papillomaviruses have a certain influence on the development of cervical neoplasias.

The objective of the investigation was to assess whether children of mothers who acquired measles antibodies resp. immunity by vaccination are at least for the first six months of their life protected by maternal antibodies. A group of fifty pregnant women mean age 18 years incl. their umbilical blood and blood of their children, mostly 5-6 months after birth, were examined by the haemagglutination inhibition and immunoenzymatic test. The levels of measles antibodies were detected in 11 women immunized against measles in cca 1970 and in 26 revaccinated women mostly after 5 to 11 years. In once vaccinated mothers and their umbilical bloods the mean HI titres were 1:8.5 and 1:14 resp. and the mean EIA titres were 1:3600 and 1:3040 resp. As to the eight newborn children at the age of 5 and 6 months 6 children did not have any protective antibodies. In twice vaccinated women and their umbilical bloods the mean HI titre was 1:10 and 1:16.4 resp. and EIA titres were 1:2937 and 1:3784 resp. Of the 15 newborn infants at the age of 4 to 6 months 11 infants did not have any protective antibodies. In 8 mothers without vaccination records and their umbilical bloods the mean HI titre was 1:7.5 and 1:25.5 and mean EIA titres were 1:8229 and 1:7360 resp. In none of their children at the age of 6 months measles antibodies were found. The finding of the lack of protection in children older than 6 months stimulates further research of the problem.

Cultivation and serological tests for Chlamydia trachomatis were made in a total of 39 male patients (24 married, 11 single, 3 divorced, 1 widower) and their female partners (9 extra-marital, 12 wives) on average 35 years old in both groups. Apart from the clinical diagnosis of non-specific urethritis found in all male patients, 11 patients suffered from other infections (5 from balanitis, 4 from prostatitis, 1 from prepuce infection, 1 from herpetic infection) and subjective disorders. Side by side with clinical diagnosis of cervicitis or in combination with urethritis found in 16 female sexual partners, two of them suffered also from vulvovaginitis, 2 from adnexitis, 1 from candidosis, 1 had dysuric difficulties and 1 female patient was found sterile. 11 women had no subjective problems. The cultivation-tinction technique used in 25 men (14 married, 11 single) and 14 female sexual partners respectively (8 marital and 6 extramarital), positive Chlamydia trachomatics was isolated 21 times (84%) and 9 times (64.3%) respectively. The immunofluorescence technique using monoclonal antibodies applied to 39 men (24 married, 15 single) and 21 female sexual partners respectively, (i. e. 12 marital and 9 extramarital) gave positive results in 29 cases (74.4%) and 16 persons (76.2%) respectively. One patient was homosexual with a positive finding in the urethra and rectum, and, in addition, he was found to have BWR positive with Candida albicans and group B streptococci, however, his serological HIV was negative. The immunoenzymatic test (EIA) showed borderline titres as being positive in men in 28.2% cases and women in 26.3% and high levels (1:128+) in 41.0% in male patients and 21.1 percent in female patients respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

The authors present the results of examination of 121 probands by the rubella test of radial haemolysis (RH), using capillary blood (direct test), as compared with the use of serum from venous blood in correlation with the rubella haemagglutination inhibition test. The diameter of the haemolytic zone, 7 mm, was as compared with the haemagglutination inhibition test in both modifications in 95% and the diameter 8 mm was seropositive in 100%. Haemolytic zones up to 6 mm must be considered seronegative even when some of the bloods or sera have bonder line haemagglutination inhibition titres. Seronegativity was expressed in the direct test of radial haemolysis by larger haemolytic zones. The specificity of the direct test of radial haemolysis, even when serum was used, was 87.5%, the sensitivity 88.2% and 88.4% resp. The reliability of positive diameters of haemolytic zones (7 mm+) is 99%, while that of negative ones (2-6 mm) only 35%. The less laborious direct test of RH proved equivalent to the radial haemolysis test using serum, as far as screening with the purpose of aimed immunization against rubella is concerned.

The authors report on the elimination surveillance programme initiated in 1969 with the introduction of mass vaccination. In 1982 the elimination of measles was achieved. It included serological surveillance or/and annual serological reports, which considerably contributed to the optimum effect of vaccination and re-vaccination strategies. Within the frame work of this programme, from 1971 to 1987, approximately 40,000 probands from all regions of the Czech Socialist Republic were examined. Proof was supplied as regards low vaccination and high post-vaccination seropositivity in children. Since the elimination of measles, seropositivity in children's and adult age has been in the range of approximately 95%. The paper gives an illustration of the vaccination history in children born in the Czech Socialist Republic in 1960-1986. It also analyzes the average levels of HI antibodies in non-vaccinated persons (1:54.9) with measles infection, in vaccinated and non-vaccinated newborns (1:20.4) and in persons after a single-dose vaccination (1:12.0). From 1982, when measles was eliminated, up to 1987, 126 sporadically occurring cases with measles were reported. In some of these cases, the exact diagnosis of measles was made with the aid of serological tests (HI test, CF reaction, EIA, IF IgM tests and SPIT-HI test).